CN106385872B - Method for evaluating salt tolerance of seeds of herbaceous local cover plant - Google Patents
Method for evaluating salt tolerance of seeds of herbaceous local cover plant Download PDFInfo
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- CN106385872B CN106385872B CN201610807363.5A CN201610807363A CN106385872B CN 106385872 B CN106385872 B CN 106385872B CN 201610807363 A CN201610807363 A CN 201610807363A CN 106385872 B CN106385872 B CN 106385872B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
- A01C1/025—Testing seeds for determining their viability or germination capacity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06Q—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES; SYSTEMS OR METHODS SPECIALLY ADAPTED FOR ADMINISTRATIVE, COMMERCIAL, FINANCIAL, MANAGERIAL OR SUPERVISORY PURPOSES, NOT OTHERWISE PROVIDED FOR
- G06Q50/00—Systems or methods specially adapted for specific business sectors, e.g. utilities or tourism
- G06Q50/02—Agriculture; Fishing; Mining
Abstract
The invention belongs to the field of plant salt tolerance screening, and provides a method for evaluating the salt tolerance of seeds of a herbaceous local cover plant. The method comprises the steps of utilizing a tubular container with an opening at the upper part as a culture container, carrying out salt stress treatment on seeds sowed in the culture container by preparing single salt solutions with different concentrations in a greenhouse, counting the germination number of the seeds in a salt stress treatment period, and then carrying out seed germination evaluation and salt tolerance evaluation. The invention overcomes the defects of complex screening experiment procedure, time consumption, more manpower consumption and higher cost in the prior art. The method can be widely used for evaluating the salt tolerance of the plant seeds in the germination stage and the seedling stage.
Description
Technical Field
The invention relates to the field of plant salt tolerance screening, in particular to a method for evaluating the salt tolerance of seeds of a herbaceous tunic plant.
Background
At present, because the ecological environment is continuously deteriorated and unreasonable development and utilization are carried out by people, the soil salinization of arid and semi-arid regions is increasingly aggravated, and the agricultural development is severely restricted. According to statistics, 1 hundred million acres of saline soil and more than 5 hundred million acres of saline-alkali wasteland are mainly distributed in dozens of provinces (regions) in the north, and only the shoal area occupies 17.35 percent of the total area of the coast; with the continuous use of chemical fertilizers and the development of irrigation agriculture, the area of secondary saline-alkali soil is continuously enlarged.
The herbaceous vegetation has super strong adaptability and is a green manure crop, so the herbaceous vegetation is also a crop which is very suitable for being popularized and planted in saline-alkali soil, is more applied to low-yield fields and saline-alkali soil planting in northern reconstruction, improves soil fertility, reduces soil salt content and achieves remarkable effect. In recent years, many herbaceous vegetation plant varieties are introduced from abroad, but the comparative study on the saline-alkali resistance of the introduced varieties is not deep enough, and the study on the theoretical basis for improving the saline-alkali soil is lacked.
Due to the fact that the saline-alkali degree of the middle and low-yield field and the saline-alkali soil is high, screening out herbaceous vegetation plant varieties with better salt tolerance is very meaningful for popularization and planting of herbaceous vegetation plants and further saline-alkali soil reformation. The germination of the seeds of herbaceous plants is the most sensitive period to salt stress in the growth and development process of the plants, and the salt stress can reduce the germination rate of the seeds and delay the germination time or reduce the germination potential, so that the research on the salt stress of the seeds of herbaceous plants in the germination period is necessary. However, the current method for evaluating the salt tolerance of the germinated plants is to culture the seeds in a light incubator with a petri dish. However, these methods are complicated, time-consuming, labor-intensive, and costly. If the prior art needs to perform seed disinfection and culture dish disinfection, especially when testing the salt tolerance of a plurality of varieties, such as the test of testing 6 concentration gradients of 3 salt solutions of 10 varieties, each treatment is repeated for 4 times, 720 product culture dishes need to be prepared, in addition, the seed placement, the daily observation and the solution supplement need to be performed one by one culture dish, and only the seed placement is predicted to be completed by 4 persons for 2 days, so that the problem of inconsistent seed germination periods can be caused.
Therefore, the development of a method capable of rapidly screening salt-tolerant herbaceous vegetation is an urgent technical problem.
In view of the above, the present invention is particularly proposed.
Disclosure of Invention
The invention aims to provide a method for evaluating the salt tolerance of herbaceous vegetation seeds, in the method, a tubular container is used as a culture container, a sand culture method is adopted for germination test, seed disinfection and seed placement are not needed, and salt solution is sprayed by a sprayer, so that a set of method for evaluating the salt tolerance of herbaceous vegetation seeds is formed, the technical problem that the screening method in the prior art needs more materials and consumes long time at the same time is solved, and the limitations of conditions such as culture dishes and culture boxes and complicated processes are eliminated. The method has the advantages of simple and convenient cultivation method, scientific screening method, high efficiency, accurate result and the like.
In order to achieve the above purpose of the present invention, the following technical solutions are adopted:
a method for evaluating salt tolerance of seeds of a herbaceous vegetation, comprising the following steps:
(1) preparation of culture vessels and culture media: the culture container is a tubular container which is transversely arranged and has an opening at the upper part, and two ends are sealed; the culture medium is dry fine river sand;
(2) preparing a salt solution: preparing single salt solutions with different concentration gradients by using a mass concentration method;
(3) sowing and salt stress treatment: filling the fine river sand in the step (1) into the culture container in the step (1), wherein the filling depth of the fine river sand is at least 10mm from an opening at the upper part of the tubular container, broadcasting herbaceous vegetation seeds in the culture container, covering the culture container with sand of 2-5mm, spraying a salt solution by using a spraying pot every day to perform salt stress treatment to serve as an experimental group, spraying water by using the spraying pot to serve as a control group, and simultaneously, culturing in a greenhouse;
(4) and (3) germination counting: continuously observing in the salt stress treatment period, and respectively counting the germination numbers in the control group and the experimental group;
(5) germination evaluation and salt tolerance evaluation: and (4) calculating the germination rate and the germination potential of the seeds according to the germination number counted in the step (4), further calculating the relative germination rate, the relative salt damage rate and the salt damage index, and finishing the germination and salt tolerance evaluation of the seeds.
In the present invention, salt tolerance evaluation is performed on herbaceous vegetation seeds, because seed germination is the most sensitive period to salt stress during plant growth and development, and salt stress reduces seed germination rate and delays germination time or reduces germination vigor. The technical scheme adopted by the invention is that different salt environments of coastal saline-alkali soil and inland saline-alkali soil are simulated manually, and the influence degree of the germination rate and the germination potential of the material to be tested under the salt stress condition relative to the non-stress condition is measured, wherein the more serious the reduction of the indexes is, the larger the salt damage degree is, the worse the salt tolerance is. And calculating salt damage indexes under different salt contents and salt stress with different concentrations by integrating the germination rate and the total germination speed of the material, and finally comparing and determining the salt tolerance of the variety.
In the invention, the seed germination evaluation in the step (5) is specifically as follows: judged by the germination rate and the germination potential of the seeds,
the germination rate of the seeds is equal to the number of all normal germination seeds/the number of seeds for detection multiplied by 100 percent in the salt stress treatment period.
The germination potential is equal to the number of all normal germination seeds/the number of seeds for detection multiplied by 100% in the salt stress treatment period.
The more serious the reduction of the germination rate and germination potential indexes of the seeds, the larger the salt damage degree of the seeds, and the worse the salt tolerance of the seeds.
In the present invention, the evaluation of the salt tolerance of the seeds in the step (5) is specifically: calculating relative germination rate, relative salt damage rate and salt damage index according to the results of the germination rate and germination vigor of the seeds, judging the salt tolerance of the seeds,
relative germination rate (%) -. normal germination seed number in salt stress treatment period/normal germination seed number under normal condition × 100%
Relative salt damage rate: RSji＝100×(X’ji-Xji)/X’ji
Salt damage index:
in the formula: RSjiIs the relative salt damage rate of the j character of the i material,
Xjiis the average value of the measured values of the j trait of the i material under the salt stress condition,
X’jithe average value of the measured values of the properties of the i material j under normal culture conditions,
Yithe salt damage index of the i material.
In the present invention, the normal culture conditions are differences from the above-mentioned salt stress culture conditions, i.e., herbaceous plants are cultured with water or a culture solution, and the control group is provided in the present invention in order to obtain X 'under the normal culture conditions'jiThe value is obtained.
In the present invention, i is a herb seed, and j is the germination rate or germination vigor of the herb seed.
When i is the herbaceous vegetation seed and j is the germination rate of the herbaceous vegetation seed:
RSjirelative salt damage rate of germination rate of herbaceous plant seeds;
Xjian average of measurements of germination rates of herbaceous plant seeds under salt stress conditions;
X’jithe average value of the measured value of the germination rate of the herbaceous vegetation seeds under the normal culture condition;
Yiis the salt damage index of herbaceous plant seeds.
When i is the herbaceous vegetation seed and j is the germination potential of the herbaceous vegetation seed:
RSjithe relative salt damage rate of the germination potential of the herbaceous plant seeds;
Xjiaverage of measurements of the germination potential of herbaceous plant seeds under salt stress conditions;
X’jithe average value of the measured value of the germination potential of the herbaceous vegetation seeds under the normal culture condition;
Yiis the salt damage index of herbaceous plant seeds.
And (4) judging a result: y isiThe larger the value, the more the material is damaged by salt damage, conversely, YiA smaller value indicates a stronger salt resistance of the material. And (4) dividing the salt tolerance grade according to the salt damage index and the following standard, wherein the numerical value is accurate to 1 bit after the decimal point.
The salt damage index is more than or equal to 0 and is more than or equal to YiIf the salt tolerance is less than 20 percent, the salt tolerance grade is grade 1, and the salt tolerance is high;
the salt damage index is more than or equal to 20 and YiIf the salt tolerance is less than 40%, the salt tolerance grade is grade 2;
the salt damage index is more than or equal to 40 and YiIf the salt tolerance is less than 60 percent, the salt tolerance is rated as grade 3, and the salt tolerance is medium;
the salt damage index is more than or equal to 60 and is YiIf the salt tolerance is less than 80%, the salt tolerance grade is grade 4, and the salt tolerance grade is sensitive to salt;
the salt damage index is more than or equal to 80 and YiThe salt tolerance grade is 5 grade when the content is less than or equal to 100 percent, and the salt is extremely sensitive.
In the present invention, the larger the above-mentioned salt damage index value is, the more serious the salt damage degree is, and the worse the salt tolerance is. And calculating salt damage indexes under different salt contents and salt stress with different concentrations by integrating the germination rate and the total germination speed of the material, and finally comparing and determining the salt tolerance of the variety.
In the invention, the method for evaluating the salt tolerance is carried out in a greenhouse, the illumination condition and the day-night temperature difference change are controllable, and the method basically accords with the culture condition of an illumination incubator. Therefore, it can also be used to measure the salt tolerance of the seeds at the seedling stage or at the continued observation of the seedling stage.
In the invention, the tubular container in the step (1) is a tubular container with two ends of a pipe orifice sealed by pipe hoops and an upper opening of a pipe body transversely placed in parallel. The openings may be perforated or slotted.
In the invention, the fine river sand in the step (1) is dried fine river sand which is repeatedly washed by clean water before use and is dried for later use.
In the present invention, the mass concentration method in the step (2) is a method of preparing a solution by using a mass concentration calculation formula of a substance, and specifically, the mass concentration of the solute is equal to the mass concentration of the solute x the volume of the solution.
In the present invention, the salt stress treatment in the step (3) is carried out by spraying the seeds in the culture vessel where the seeds are sown with a watering can on a daily basis. Compared with the prior art which uses a dropper method for culturing, the method for culturing the salt stress by spraying the salt solution by using the watering can has the advantages that the spraying is more uniform, the salt solution is directly sprayed on the leaves, the absorption efficiency is higher, the experimental time is indirectly saved, the method is more suitable for large-scale culture, and the labor cost is also saved.
In the present invention, the spraying amount of the monosalt solution is sprayed daily by a spraying pot in the step (3) to keep the culture substrate moist and free from water accumulation.
In the invention, the greenhouse in the step (3) manually regulates and controls the illumination condition and the day and night temperature difference change in the greenhouse so as to achieve the culture condition of the illumination incubator in the prior art, thereby overcoming the limitation of the illumination incubator in the prior art.
The culture method used in the invention is a sand culture method, and is a method for culturing the flower plants by taking sand, perlite, plastic or other inorganic substances with the diameter less than 3mm as a matrix and adding nutrient solution. The sand culture method has the advantages that the plants are easy to root and have no risk of root rot.
Further, the culture container is a tubular PVC container which is transversely placed, sealed at two ends and provided with an opening at the upper part.
In the invention, the tubular PVC container is a PVC pipe. PVC is a vinyl polymer material which is a non-crystalline material having non-flammability, high strength, weathering resistance and excellent geometric stability, and is very resistant to oxidizing agents, reducing agents and strong acids. The invention can use waste PVC pipe to save cost, change waste into valuable and recycle PVC material.
According to the invention, the length of the transversely placed tubular PVC container with the upper opening can be adjusted according to the requirements of a culture experiment, when salt-tolerant screening of herbaceous plant seed materials of more than 8 varieties is carried out, the tubular PVC container with the upper opening and the length of 800-1200 mm can be selected, then, the tubular PVC container with the upper opening is averagely divided into 8-10 parts, each division area is 10-15 mm, each variety is randomly arranged and sown on one division area, and the edge of each variety is marked with a label.
Furthermore, the diameter of the tubular PVC container with the upper opening and two sealed ends transversely arranged is 140-180 mm, the width of the upper opening is 100-150 mm, the depth is 120-150 mm, and the length is 800-1200 mm.
Further, the transversely placed tubular PVC container with both sealed ends and an upper opening has the diameter of 160mm, the width of the upper opening of 110mm, the depth of 130mm and the length of 1100 mm.
In the invention, if the culture container is used for measuring the salt tolerance of herbaceous vegetation germination period, the culture container can select a PVC pipe with the diameter of 110mm, divide the PVC pipe into two parts, cut the required length, and install pipe hoops at two ends for sealing, and the division of the PVC pipe with the diameter of 110mm into two parts means that the PVC pipe is cut and flattened by taking the transverse central axis of the PVC pipe as a reference to form two groove-shaped culture containers, so that the use of the PVC pipe can be saved, and the experiment cost is saved.
Further, the monosalt in the step (2) is NaCl or Na2CO3Or NaHCO3。
Further, the single salt is prepared into salt solutions with different concentration gradients.
In the invention, salt solutions with different concentration gradients are prepared to simulate saline-alkali land in different areas of China and the corresponding crop growth environment, wherein NaCl is neutral salt, and NaHCO is used as the salt solution3、Na2CO3Is a baseAnd (4) neutral salt.
Preferably, the concentration gradient of the single salt in the step (2) is 5, and the concentration gradient is 0.3%, 0.6%, 0.9%, 1.2% and 1.5%.
Further, the culture vessel in the above step (3) is divided into at least two parts.
In the invention, the culture container is divided into a plurality of parts of at least two parts, because the culture container is mainly used for screening the salt resistance of the seeds, the problem that the seeds of a plurality of varieties are simultaneously and synchronously cultured under the same condition is solved, the culture container is divided into a plurality of parts of at least two parts, the seeds of different types are scattered into different divided parts for culture, the conditions such as the growth environment and the like of the seeds of different types are the same, and the consistency of screening experiment conditions is favorably kept.
Further, the salt stress treatment was performed under the condition of alternating periods of illumination of 12/12h in a greenhouse.
In the invention, the condition of the illumination alternation period 12/12h in the greenhouse simulates the illumination alternation period environment of placing the seed culture dish in the illumination incubator in the prior art, thereby overcoming the limitation of the illumination incubator in the prior art.
Further, the salt stress treatment is carried out at an ambient temperature of 15-25 ℃ and an ambient humidity of 40-60%.
In the invention, the conditions that the environmental temperature is 15-25 ℃ and the environmental humidity is 40-60% are that the environmental temperature and the humidity of the seed culture dish placed in the illumination incubator in the prior art are simulated, so that the limitation of the illumination incubator in the prior art is overcome.
Further, the herbaceous vegetation is alfalfa.
The alfalfa is a leguminous herbaceous plant, is a high-quality and high-yield crop with a large artificial planting area in China, has the advantages of strong adaptability, multiple utilization modes and high economic value, and has great significance in improving saline-alkali soil and developing saline-alkali soil animal husbandry.
Further, the method for evaluating the salt tolerance of the herbaceous plant seeds comprises the following steps:
(1) preparation of culture vessel and culture Medium
Selecting a tubular PVC container with the diameter of 140-180 mm, the width of an upper opening of 100-150 mm and the length of 800-1200 mm, transversely placing the container, and sealing two ends of the container by using pipe hoops; selecting culture medium, repeatedly washing with clear water, and drying to obtain fine river sand;
(2) formulating a salt solution
Preparing single salt solutions with concentration gradients of 0.3%, 0.6%, 0.9%, 1.2% and 1.5% by mass concentration method, wherein the single salt is NaCl and Na2CO3Or NaHCO3；
(3) Seeding and salt stress treatment
Filling the fine river sand into the tubular PVC container which is transversely placed and provided with an opening at the upper part, wherein the filling depth of the fine river sand is 10mm away from the upper opening of the container, evenly dividing the container into 8-12 parts, each divided area is 10-15 mm, broadcasting different types of seeds in the divided areas, marking labels at the edges of the divided areas, covering 2-5mm of sand, spraying a single salt solution by using a spraying pot every day to perform salt stress treatment to serve as an experimental group, spraying water by using the spraying pot to serve as a control group, and simultaneously culturing in a greenhouse;
the environment temperature for cultivation in the greenhouse is 15-25 ℃, the environment humidity is 40-60%, and the illumination condition is an illumination alternating period of 12/12 hours;
(4) germination counting
Continuously observing in the salt stress treatment period, and respectively counting the germination numbers in the control group and the experimental group;
(5) germination evaluation and salt tolerance evaluation
And calculating the germination rate and the germination potential of the seeds according to the counted germination number, further calculating the relative germination rate, the relative salt damage rate and the salt damage index, and finishing the germination and salt tolerance evaluation of the seeds.
Then, the germination evaluation is carried out on the seeds through the germination rate and the germination vigor of the seeds, and specifically, the germination evaluation is carried out on the seeds through the following steps:
the germination rate of the seeds is equal to the number of all normal germination seeds/the number of seeds for detection multiplied by 100 percent in the salt stress treatment period.
The germination potential is equal to the number of all normal germination seeds/the number of seeds for detection multiplied by 100% in the salt stress treatment period.
The more serious the reduction of the germination rate and germination potential indexes of the seeds, the larger the salt damage degree of the seeds, and the worse the salt tolerance of the seeds.
Calculating the relative germination rate, the relative salt damage rate and the salt damage index according to the results of the germination rate and the germination vigor of the seeds, and judging the salt tolerance of the seeds, which specifically comprises the following steps:
relative germination rate (%) -. normal germination seed number in salt stress treatment period/normal germination seed number under normal condition × 100%
Relative salt damage rate: RSji＝100×(X’ji-Xji)/X’ji
Salt damage index:
in the formula: RSjiIs the relative salt damage rate of the j character of the i material,
Xjiis the average value of the measured values of the j trait of the i material under the salt stress condition,
X’jithe average value of the measured values of the properties of the i material j under normal culture conditions,
Yithe salt damage index of the i material.
In the present invention, i is a herb seed, and j is the germination rate or germination vigor of the herb seed.
When i is the herbaceous vegetation seed and j is the germination rate of the herbaceous vegetation seed:
RSjirelative salt damage rate of germination rate of herbaceous plant seeds;
Xjian average of measurements of germination rates of herbaceous plant seeds under salt stress conditions;
X’jithe average value of the measured value of the germination rate of the herbaceous vegetation seeds under normal conditions;
Yiis a herbaceous ground quiltSalt damage index of plant seeds.
When i is the herbaceous vegetation seed and j is the germination potential of the herbaceous vegetation seed:
RSjithe relative salt damage rate of the germination potential of the herbaceous plant seeds;
Xjiaverage of measurements of the germination potential of herbaceous plant seeds under salt stress conditions;
X’jithe average value of the measured value of the germination potential of the herbaceous vegetation seeds under the normal culture condition;
Yiis the salt damage index of herbaceous plant seeds.
And (4) judging a result: y isiThe larger the value, the more the material is damaged by salt damage, conversely, YiA smaller value indicates a stronger salt resistance of the material. And (4) dividing the salt tolerance grade according to the salt damage index and the following standard, wherein the numerical value is accurate to 1 bit after the decimal point.
The salt damage index is more than or equal to 0 and is more than or equal to YiIf the salt tolerance is less than 20 percent, the salt tolerance is rated as 1 grade, and the salt tolerance is high;
the salt damage index is more than or equal to 20 and YiIf the salt tolerance is less than 40%, the salt tolerance grade is grade 2;
the salt damage index is more than or equal to 40 and YiIf the salt tolerance is less than 60 percent, the salt tolerance is rated as grade 3, and the salt tolerance is medium;
the salt damage index is more than or equal to 60 and is YiIf the salt tolerance is less than 80%, the salt tolerance grade is grade 4, and the salt tolerance grade is sensitive to salt;
the salt damage index is more than or equal to 80 and YiThe salt tolerance grade is 5 grade when the content is less than or equal to 100 percent, and the salt is extremely sensitive.
In the present invention, the larger the above-mentioned salt damage index value is, the more serious the salt damage degree is, and the worse the salt tolerance is. And calculating salt damage indexes under different salt contents and salt stress with different concentrations by integrating the germination rate and the total germination speed of the material, and finally comparing and determining the salt tolerance of the variety.
Compared with the prior art, the invention has the beneficial effects that:
(1) the salt tolerance screening method disclosed by the invention is used for screening the herbaceous vegetation in the tubular container by using a sand culture method, and the defects of complex screening experiment procedure, more time consumption, more manpower consumption and higher cost in the prior art are overcome. In the prior art, a screening experiment needs to perform seed disinfection and culture dish disinfection, particularly, when salt tolerance of herbaceous ground cover plants of multiple varieties is tested, for example, a test of 6 concentration gradients of 3 salt solutions of 10 varieties is tested, each treatment is repeated for 4 times, 720 culture dishes need to be prepared, in addition, seed placement, daily observation and solution supplement need to be performed one by one in culture dish, only seed placement is predicted to be completed by 4 persons for 2 days, and thus the problem of inconsistent seed germination periods can be caused. According to the method, the sand culture method is used for culturing the herbaceous vegetation seeds, so that the work of seed disinfection and liquid supplementation of a culture dish is omitted, the tubular container is used for culturing the herbaceous vegetation seeds, the work of disinfecting the culture dish and placing the seeds is also omitted, 3-5 workers can be directly saved, and the time is saved by 5-10 days.
(2) When herbaceous vegetation is cultivated, the salt stress treatment is carried out by spraying the salt solution by using the watering pot, compared with the salt stress treatment carried out by using the dropper in the prior art, the salt stress treatment method has the advantages of uniform spraying, time saving and labor saving, reduces the manual investment compared with the traditional dropper method, and shortens the identification period.
(3) The process of screening the salt tolerance of herbaceous vegetation is carried out in a greenhouse, the temperature and humidity conditions in the greenhouse are controllable, and the illumination conditions and the day-night temperature difference change basically accord with the conditions of an illumination incubator, so that the condition constraint of the incubator is eliminated.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
Example 1
A method for screening salt-tolerant herbaceous vegetation, taking alfalfa as an example, comprises the following steps:
1. customized culture vessels and culture matrices
Selecting a PVC (polyvinyl chloride) tube container with the diameter of 160mm, wherein the opening on the container is 110mm wide, 130mm deep and 1100mm long, the two ends of the container are sealed by tube hoops, and the test needs 72 containers in total; selecting fine river sand as a culture medium, repeatedly washing with clear water before use, and airing for later use.
2. Pretreatment of materials
Preparing a NaCl salt solution: preparing a NaCl salt solution by using a mass concentration method, and setting 5 concentration gradients: 0.3%, 0.6%, 0.9%, 1.2% and 1.5%, with distilled water as a control treatment (0%).
Seed selection: selecting 10 alfalfa varieties, which specifically comprise: alfalfa seeds of 10 alfalfa varieties including chinese alfalfa seeds, alai rouge, Aohan, predecessor, royal, WL, debao, Sandeli, surprise and Sedi are respectively recorded as 1-10 groups, and the details are shown in Table 1.
The selected seeds are required to be bright and full in color, every 100 seeds of each variety are put into a small plastic package bag for storage, corresponding parts are counted according to test requirements, and labels are marked for later use. Each variety in the test is 72 parts, and the total amount is 720 parts.
Table 1:
3. seeding and salt stress treatment
And (3) filling the cleaned fine river sand into the PVC container, paving and flattening the sand, wherein the depth of the sand is 120mm, namely, a distance of 10mm is reserved from an upper opening, each NaCl salt treatment is repeated for 4 times, namely 4 containers are used, and the variety to be tested is cultured in each container.
Each container is divided into 10 parts on average, each divided area is 10mm, each variety is randomly arranged and sowed on one area, the edge is marked with a label, the container is thoroughly watered with clear water before sowing, when no water is accumulated on the surface, 100 selected seeds are uniformly sowed in the divided areas, fine river sand with the thickness of 2-5mm is covered, then salt solutions with various concentrations are sprayed by a small spray can, and the container is kept moist without water accumulation.
And (3) maintaining the environment temperature at 20 ℃ and the environment humidity at 50% in a greenhouse, and performing germination culture under the condition of an illumination alternation period of 12/12 h.
4. Germination determination and evaluation
Spraying saline solution every day after sowing, recording the seedling emergence condition every day, pulling out the seedlings by using tweezers after seedling emergence, continuously growing seedlings on the 2 nd to 6 th days, and observing for 10 days continuously until no seedling emergence exists.
And then, carrying out germination evaluation on the seeds stressed by the sodium chloride (NaCl) salt solution, and calculating the germination rate and the germination potential of the seeds to calculate the relative germination rate.
The germination rate of the seeds was defined as the number of all normal germinated seeds/test seeds at the final germination stage (day 10) × 100% (4 replicates in total, and the final germination rate of the seeds was averaged).
The germination potential is the number of all normal germination grains/test seeds within a specified date multiplied by 100% (4 replicates in total, and the test calculates the germination condition in the first 7 days after germination).
Relative germination (%) - (number of germinated seeds/number of germinated seeds in control group) × 100%
The effect of sodium chloride (NaCl) salt solution on the relative germination (%) of the different alfalfa varieties in this example is shown in Table 2:
table 2:
as can be seen from Table 2, the relative germination rates of 10 alfalfa varieties are significantly inhibited with increasing NaCl concentration, and NaCl salt stress reduces the germination rates of all varieties.
At concentrations of 0.3% and 0.6% treatment compared to the control, no significant reduction in other species occurred, except for the 0.6% concentration at which alharan and Aohan had been significantly reduced; the significantly reduced concentration of alungau and Aohan was 0.6%; the obvious reduced concentration of Sandeli, WL, queen and Saidi is 0.9%, and the obvious reduced concentration of the Chinese alfalfa I, the antecedent, the surprise and the Debao is 1.2%; 1.2% and above concentration make the relative germination percentage of all varieties all show and reduce, wherein the decline range of chinese alfalfa one is minimum, and relative germination percentage still is close to 50% when 1.5%.
Through comparative analysis of different varieties under the same concentration, only the alfalfa I and WL are slightly higher when the concentration is 0.3%, and the difference among the varieties is not obvious; when the concentration reaches 0.6%, the difference among varieties is obvious (p is less than 0.05), wherein the other varieties of Alaskin, Aohan and Sedi; when the concentration reaches 0.9%, the difference among the varieties also reaches a remarkable level (p is less than 0.01), and the alfalfa I, Debao, surprise, predecessor, queen and WL are remarkably higher than other alfalfa varieties, which indicates that the NaCl salt resistance of the varieties to seeds is higher than that of other varieties under the treatment of 0.9%; when the NaCl concentration is increased to 1.2%, the relative germination rates of the alfalfa I, Debao and predecessors are the highest, and the surprise, the queen and the WL are slightly lower than those of the former varieties, but the difference is not obvious; the relative germination rates of the Alaska, the Sanderli and the Aohan are gradually and obviously reduced when the alfalfa is obviously higher than other alfalfa varieties and Sedi; when the NaCl concentration is increased to 1.5%, the difference between the varieties is obvious (p <0.001), and the relative germination rates of the varieties are in turn from big to small, namely alfalfa I, Debao, Prime, Sedidy, Huanghou, Algang, WL, Sandeli, and Aohan.
Comprehensive analysis can show that the alfalfa I has the best NaCl salt tolerance, the Debao, surprise and predecessor have the better salt tolerance and the Aohan has the worst NaCl salt tolerance in the seed germination period.
Then, the seeds were evaluated for salt tolerance, and the relative salt damage rate and the salt damage index were calculated from the germination rate, germination potential and relative germination rate as described above.
And calculating the salt damage index of each material by using a formula, and determining the salt tolerance of the material.
Relative salt damage rate: RSji＝100×(X’ji-Xji)/X’ji
Salt damage index:
in the formula: RSjiIs the relative salt damage rate of the j character of the i material,
Xjiis the average value of the measured values of the j trait of the i material under the salt stress condition,
X’jithe average value of the measured values of the j properties of the i material under normal conditions,
Yithe salt damage index of the i material.
In the present invention, i is a herb seed, and j is the germination rate or germination vigor of the herb seed.
When i is the herbaceous vegetation seed and j is the germination rate of the herbaceous vegetation seed:
RSjirelative salt damage rate of germination rate of herbaceous plant seeds;
Xjian average of measurements of germination rates of herbaceous plant seeds under salt stress conditions;
X’jithe average value of the measured value of the germination rate of the herbaceous vegetation seeds under normal conditions;
Yiis the salt damage index of herbaceous plant seeds.
When i is the herbaceous vegetation seed and j is the germination potential of the herbaceous vegetation seed:
RSjithe relative salt damage rate of the germination potential of the herbaceous plant seeds;
Xjiaverage of measurements of the germination potential of herbaceous plant seeds under salt stress conditions;
X’jithe average value of the measured value of the germination potential of the herbaceous vegetation seeds under normal conditions;
Yiis the salt damage index of herbaceous plant seeds.
And (4) judging a result: y isiThe larger the value, the more the material is damaged by salt damage, conversely, YiA smaller value indicates a stronger salt resistance of the material. According to the salt damage index, the salt tolerance grade and number are divided according to the following standardsThe value is accurate to 1 bit after the decimal point.
The relative salt damage rates of the sodium chloride (NaCl) salt solution in this example to the germination rates of the different alfalfa varieties are shown in Table 3:
table 3:
the relative salt damage rates to the germination vigour of the different alfalfa varieties under stress of sodium chloride (NaCl) salt solution in this example are shown in table 4:
table 4:
in this example, the salt damage index of sodium chloride (NaCl) salt solution under stress to the above different alfalfa varieties is shown in table 5:
table 5:
as shown in the above tables 3 and 4, the relative salt damage germination rates and germination potentials of different alfalfa varieties under NaCl salt solution stress are obtained according to the relative salt damage rate formula, and the relative salt damage rates are obviously enhanced along with the increase of the salt concentration.
As shown in Table 5, the salt tolerance of different varieties is different under different NaCl salt solution stress concentrations,
all varieties have the salt tolerance level of 1 under the stress of 0.3 percent NaCl salt solution, and the salt tolerance is high to the concentration;
under the stress of 0.6% NaCl salt solution, the grades of the alfalfa I, the predecessor, the queen, the WL, the Debao, the Sandeli and the surprise salt tolerance are 1 grade, which shows high tolerance, while the grades of the Aohan, the Aohang and the Sedi salt tolerance are 2, which shows salt tolerance, but are lower than other varieties;
the grades of alfalfa I, Debao and surprise salt tolerance are 1 grade under the stress of 0.9 percent NaCl salt solution, and the alfalfa salt still has high tolerance to the concentration; the salt tolerance level of the predecessor, the queen, the WL, the Sandeli and the Sedi is 2 grade, the salt tolerance is shown, the salt tolerance level of the Algang gold is 3 grade, and the salt tolerance level is middle grade; aohan salt tolerance grade is 4 grades, and the Aohan salt tolerance grade shows that the Aohan salt tolerance grade is sensitive to salt at the concentration;
the grades of the alfalfa I, WL, predecessors, Debao, surprise and Sedi salt tolerance are 3 grades under the stress of 1.2 percent NaCl salt solution, and the medium tolerance is still expressed to the concentration; the salt tolerance grades of Sandeli, queen and Alaskin are 4 grades and are sensitive to salt; aohan salt tolerance grade is 5 grades, and the Aohan salt tolerance grade is extremely sensitive to salt at the concentration;
the salt tolerance grades of alfalfa I and Debao are 4 grades under the stress of 1.5 percent NaCl salt solution, and the alfalfa I and the Debao are sensitive to the salt solution with the concentration; the salt tolerance level of Alaskin, predecessor, queen, WL, Sandeli, surprise, Sedi and Aohan is 5 grade, and the salt tolerance level shows that the salt tolerance level is extremely sensitive.
Example 2
A method for screening salt-tolerant herbaceous vegetation, taking alfalfa as an example, comprises the following steps:
1. customized culture vessels and culture matrices
Selecting a PVC (polyvinyl chloride) tube container with the diameter of 160mm, wherein the opening on the container is 110mm wide, 130mm deep and 1100mm long, the two ends of the container are sealed by tube hoops, and the test needs 72 containers in total; selecting fine river sand as a culture medium, repeatedly washing with clear water before use, and airing for later use.
2. Pretreatment of materials
Preparation of sodium bicarbonate (NaHCO)3) Salt solution: preparation of NaHCO by mass concentration method3Saline solution, divided into 5 concentration gradients: 0.3%, 0.6%, 0.9%, 1.2% and 1.5%, with distilled water as a control treatment (0%).
Seed selection: selecting 10 alfalfa varieties, which specifically comprise: alfalfa seeds of 10 alfalfa varieties of Zhonghui, Aergang, Aohan, predecessor, Huanghou, WL, Debao, Sandeli, Jingxi and Sedi are respectively recorded as 1-10 groups, which is specifically shown in Table 1 in the above examples.
The selected seeds are required to be bright and full in color, every 100 seeds of each variety are put into a small plastic package bag for storage, corresponding parts are counted according to test requirements, and labels are marked for later use. Each variety in the test is 72 parts, and the total amount is 720 parts.
3. Seeding and salt stress treatment
And (3) filling the cleaned fine river sand into the PVC container, wherein the sand needs to be paved and leveled, and the depth is 120mm, namely, the distance of 10mm is reserved from the upper opening. Each NaHCO3The salt treatment was repeated 4 times, i.e.4 vessels were used, each vessel being incubated with the species to be tested.
Each container is divided into 10 parts on average, each divided area is 10mm, each variety is randomly arranged and sowed on one area, the edge is marked with a label, the container is thoroughly watered with clear water before sowing, when no water is accumulated on the surface, 100 selected seeds are uniformly sowed in the divided areas, fine river sand with the thickness of 2-5mm is covered, then salt solutions with various concentrations are sprayed by a spray can, and the container is kept moist without water accumulation.
And (3) maintaining the environment temperature at 20 ℃ and the environment humidity at 50% in a greenhouse, and performing germination culture under the condition of an illumination alternation period of 12/12 h.
5. Germination determination and evaluation
Spraying saline solution every day after sowing, recording the seedling emergence condition every day, pulling out the seedlings by using tweezers after seedling emergence, continuously growing seedlings on the 2 nd to 6 th days, and observing for 10 days continuously until no seedling emergence exists.
Then, for NaHCO3And (4) carrying out germination evaluation on the seeds stressed by the salt solution, and calculating the germination rate and the germination potential of the seeds to calculate the relative germination rate.
The calculation methods of the germination rate, germination potential and relative germination rate of the seeds are the same as those of example 1.
NaHCO in this example3The effect of salt solution on the relative germination (%) of the different alfalfa varieties as described above is shown in Table 6：
Table 6:
as can be seen from Table 6, the relative germination rates of 10 alfalfa varieties follow NaHCO3The concentration increases and decreases significantly. Comparison at the same concentration with NaHCO3At a concentration of 0.3%, the alkali resistance of 10 varieties was: the predecessor, queen, debao and surprise are not obviously reduced compared with the contrast, other varieties are obviously reduced compared with the contrast, and the relative germination rate of the Aohan alfalfa is the lowest.
In NaHCO3When the concentration is 0.6%, the relative germination rates of all varieties are obviously lower than the corresponding indexes of a control and 0.3%; the difference between the varieties is also obvious, the Sandeli and the Debao are relatively high, the alfalfa I, the predecessor, the queen, the WL and the surprise are less, the Sidi and the Algang gold are lower, and the Aohan is the lowest.
When the concentration reaches 0.9% or more, the relative germination rates of the other varieties are almost not germinated except that the relative germination rate of the debao reaches 20%, the relative germination rates are all lower than 7%, the difference among the varieties is not obvious, and the difference among the concentrations is also not obvious.
Then, the seeds were evaluated for salt tolerance, and the relative salt damage rate and the salt damage index were calculated from the germination rate, germination potential and relative germination rate as described above.
The calculation methods of the relative salt damage ratio and the salt damage index are the same as those of example 1.
NaHCO in this example3The relative salt damage rates under salt solution stress to the germination rates of the different alfalfa varieties described above are shown in table 7:
table 7:
NaHCO in this example3The relative salt damage rates under salt solution stress to the germination vigour of the different alfalfa varieties described above are shown in table 8:
table 8:
NaHCO in this example3Salt damage indices to the different alfalfa varieties described above under salt solution stress are shown in table 9:
table 9:
as can be seen from tables 7 and 8, NaHCO was obtained according to the relative salt damage ratio equation3The stress is applied to the germination rate and the germination table of the relative salt damage rate of different alfalfa varieties, the relative salt damage rate is obviously enhanced along with the increase of the salt concentration, and particularly, the salt damage rate is mostly over 50 percent when the concentration is over 0.6 percent.
As can be seen from Table 9, the various NaHCO groups3Salt tolerance of different species at stress concentration was different, 0.3% NaHCO3The salt tolerance grades of alfalfa I, antecedent, WL, queen, Debao, Sandeli, surprise and Sedi are 1 grade under stress, and the high salt tolerance is shown; the salt tolerance level of the Aergang gold and the Aohan is 2 grade, and the concentration is expressed as salt tolerance; 0.6% NaHCO3Under stress, the grades of the medium alfalfa I, the antecedent, the Debao, the Sandeli and the surprise salt tolerance are 4 grades, and the medium salt tolerance is shown; the salt tolerance grades of queen, WL, Alaskin and Sedi are 4 grades and are sensitive to salt; aohan salt tolerance grade is 5 grade, and the Aohan salt tolerance grade is extremely sensitive to salt; NaHCO at a concentration of 0.9% and above3The salt tolerance grades of all 10 varieties are 5 grades under stress, and the salt tolerance grades are extremely sensitive to salt.
Example 3
A method for screening salt-tolerant herbaceous vegetation, taking alfalfa as an example, comprises the following steps:
1. customized culture vessels and culture matrices
Selecting a PVC (polyvinyl chloride) tube container with the diameter of 160mm, wherein the opening on the container is 110mm wide, 130mm deep and 1100mm long, the two ends of the container are sealed by tube hoops, and the test needs 72 containers in total; selecting fine river sand as a culture medium, repeatedly washing with clear water before use, and airing for later use.
2. Pretreatment of materials
Preparing sodium carbonate (Na)2CO3) Salt solution: preparing Na by applying mass concentration method2CO3Saline solution, divided into 5 concentration gradients: 0.3%, 0.6%, 0.9%, 1.2% and 1.5%, with distilled water as a control treatment (0%).
Seed selection: selecting 10 alfalfa varieties, which specifically comprise: alfalfa seeds of 10 alfalfa varieties of Zhonghui, Aergang, Aohan, predecessor, Huanghou, WL, Debao, Sandeli, Jingxi and Sedi are respectively recorded as 1-10 groups, which is specifically shown in Table 1 in the above examples.
The selected seeds are required to be bright and full in color, every 100 seeds of each variety are put into a small plastic package bag for storage, corresponding parts are counted according to test requirements, and labels are marked for later use. Each variety in the test is 72 parts, and the total amount is 720 parts.
3. Seeding and salt stress treatment
And (3) filling the cleaned fine river sand into the PVC container, wherein the sand needs to be paved and leveled, and the depth is 120mm, namely, the distance of 10mm is reserved from the upper opening. Each Na2CO3The salt treatment was repeated 4 times, i.e.4 vessels were used, each vessel being incubated with the species to be tested.
Each container is divided into 10 parts on average, each divided area is 10mm, each variety is randomly arranged and sowed on one area, the edge is marked with a label, the container is thoroughly watered with clear water before sowing, when no water is accumulated on the surface, 100 selected seeds are uniformly sowed in the divided areas, fine river sand with the thickness of 2-5mm is covered, then salt solutions with various concentrations are sprayed by a spray can, and the container is kept moist without water accumulation.
And (3) maintaining the environment temperature at 20 ℃ and the environment humidity at 50% in a greenhouse, and performing germination culture under the condition of an illumination alternation period of 12/12 h.
6. Germination determination and evaluation
Spraying saline solution every day after sowing, recording the seedling emergence condition every day, pulling out the seedlings by using tweezers after seedling emergence, continuously growing seedlings on the 2 nd to 6 th days, and observing for 10 days continuously until no seedling emergence exists.
Then, for Na2CO3And (4) carrying out germination evaluation on the seeds stressed by the salt solution, and calculating the germination rate and the germination potential of the seeds to calculate the relative germination rate.
The calculation methods of the germination rate, germination potential and relative germination rate of the seeds are the same as those of example 1.
In this example Na2CO3The effect of salt solution on the relative germination (%) of the different alfalfa varieties described above is shown in table 10:
table 10:
as can be seen from Table 10, Na2CO3Salt stress significantly reduced the relative germination of all varieties. In Na2CO3The relative germination rates of 10 alfalfa varieties under stress are along with Na2CO3The increase in concentration is significantly suppressed. In addition to surprises and predecessors, the 0.3% concentration treatment has significantly reduced the relative germination rate of other varieties; when the concentration is 0.6%, the relative germination rate of all varieties is lower than 10%; when the concentration is 0.9%, only the four varieties of alfalfa I, Al gang jin, Xiu xing and Ji xi can germinate, but the germination rate is less than 1%. When the concentration is increased to 1.2%, only the predecessors and alfalfa I can germinate, and the germination rate is also less than 1%.
At the same concentration, in comparison with Na2CO3Relative germination of 10 varieties at 0.3%The rates are in the order of surprise, Debao, predecessor, Chinese alfalfa I, Huanghou, WL, Sandeli, Sedi, Alrouge and Aohan. When the concentration is 0.6%, the relative germination rate of Aohan is only 0, which is obviously lower than that of other varieties. There was no significant difference between all varieties when the concentrations were 0.9% and 1.2%. This indicates low Na2CO3The Na-resistance of Debao2CO3Is relatively good. While Na-resistant herbs of higher concentration, those of the first alfalfa, antecedent and surprise2CO3The salt property is better.
Then, the seeds were evaluated for salt tolerance, and the relative salt damage rate and the salt damage index were calculated from the germination rate, germination potential and relative germination rate as described above.
The calculation methods of the relative salt damage ratio and the salt damage index are the same as those of example 1.
In this example Na2CO3The relative salt damage rates under salt solution stress to the germination rates of the different alfalfa varieties described above are shown in table 11:
table 11:
in this example Na2CO3The relative salt damage rates under salt solution stress to the germination vigour of the different alfalfa varieties described above are shown in table 12:
table 12:
in this example Na2CO3Stress the salt solution to the above-mentioned different alfalfaSalt damage index of alfalfa varieties, as shown in table 13:
table 13:
obtaining Na according to a relative salt damage rate formula2CO3Stress to the germination rate and germination time table (table 11 and table 12) of the relative salt damage rate of different alfalfa varieties, the relative salt damage rate is remarkably enhanced along with the increase of the salt concentration, and particularly, the salt damage rate is mostly over 90 percent at the concentration of 0.6 percent or above.
Table 13 shows that Na2CO3The salt damage of different varieties is high due to stress, and only 0.3 percent of Na2CO3Partial varieties are stressed to show better salt tolerance, such as queen, debao, Sandeli and surprise salt tolerance grade of 1, and show high salt tolerance; alfalfa No. one, Algang jin, predecessors, WL and Sedi salt tolerance grade 2, which shows salt tolerance; aohan salt tolerance grade of 4, which has been shown to be sensitive to this concentration; na concentration of 0.6% or more2CO3The salt tolerance grades of all 10 varieties are 5 grades under stress, and the salt tolerance grades are extremely sensitive to salt.
Results example 1
The salt damage indexes of three salt stresses are integrated, and the alfalfa I and Debao in 10 tested varieties have the strongest comprehensive salt tolerance, the alfalfa I has the strongest neutral salt tolerance, the Debao has the strongest alkali salt tolerance, WL, queen, predecessor, Sandeli and surprise salt tolerance are analyzed, and the Aerngin and the Sedi have the salt tolerance and the Aohan salt tolerance is the worst; the influence of neutral salt (NaCl) on the germination period of alfalfa varieties is less than that of alkaline salt (NaHCO)3、Na2CO3) The injury of (1).
While particular embodiments of the present invention have been illustrated and described, it would be obvious that various other changes and modifications can be made without departing from the spirit and scope of the invention. It is therefore intended to cover in the appended claims all such changes and modifications that are within the scope of this invention.
Claims (1)
1. A method for evaluating salt tolerance of alfalfa seeds, comprising the following steps:
(1) preparation of culture vessel and culture Medium
Selecting a tubular PVC container with the diameter of 140-180 mm, the width of an upper opening of 100-150 mm and the length of 800-1200 mm, transversely placing the container, and sealing two ends of the container by using pipe hoops; selecting culture medium, repeatedly washing with clear water, and drying to obtain fine river sand;
(2) formulating a salt solution
Preparing single salt solutions with concentration gradients of 0.3%, 0.6%, 0.9%, 1.2% and 1.5% by using a mass concentration method; the monosalt is NaCl and Na2CO3Or NaHCO3；
(3) Seeding and salt stress treatment
Filling the fine river sand in the step (1) into the transversely placed tubular PVC container with the upper opening, wherein the filling depth of the fine river sand is 10mm away from the upper opening of the tubular PVC container with the upper opening, evenly dividing the tubular PVC container into 8-12 parts, each divided area is 10-15 mm, sowing different seeds in the divided areas, marking labels at the edges of the divided areas, covering 2-5mm of sand, spraying a single salt solution by a spraying pot every day to perform salt stress treatment to serve as an experimental group, spraying water by the spraying pot to serve as a control group, and simultaneously culturing in a greenhouse;
the environment temperature for cultivation in the greenhouse is 15-25 ℃, the environment humidity is 40-60%, and the illumination condition is an illumination alternating period of 12/12 hours;
(4) germination counting
Continuously observing in the salt stress treatment period, and respectively counting the germination numbers in the control group and the experimental group;
(5) germination evaluation and salt tolerance evaluation
Calculating the germination rate and the germination vigor of the seeds according to the germination number counted in the step (4), further calculating the relative germination rate, the relative salt damage rate and the salt damage index, and finishing the germination and salt tolerance evaluation of the seeds;
the germination rate of the seeds is equal to the number of all normal germination seeds/the number of seeds to be detected multiplied by 100 percent in the salt stress treatment period;
the germination potential is equal to the number of all normal germination grains/the number of seeds for detection multiplied by 100 percent in the salt stress treatment period;
the more serious the reduction of the germination rate and the germination potential indexes of the seeds, the larger the salt damage degree of the seeds, and the worse the salt tolerance of the seeds;
the relative germination rate (%) -100% of all normal germination seeds in the salt stress treatment period/100% of all normal germination seeds under normal conditions;
relative salt damage rate: RSji ═ 100 × (X 'ji-Xji)/X' ji;
salt damage index:
in the formula: RSji is the relative salt damage rate of i material j character,
xji is the average value of the measured values of i material j trait under salt stress condition,
x' ji is the average value of the measured values of the properties of the i material j under normal culture conditions,
yi is the salt damage index of the i material;
wherein, i is alfalfa seeds, and j is the germination percentage or germination vigor of the alfalfa seeds;
when i is alfalfa seed, j is alfalfa seed's germination percentage:
RSji is the relative salt damage rate of the germination rate of alfalfa seeds;
xji average value of the measured value of germination rate of alfalfa seeds under salt stress condition;
x' ji is the average value of the measured value of the germination rate of the alfalfa seeds under the normal culture condition;
yi is the salt damage index of the alfalfa seeds;
when i is alfalfa seed, j is alfalfa seed's germinating potential:
RSji is the relative salt damage rate of the germination potential of the alfalfa seeds;
xji average value of the measurement value of germination potential of alfalfa seeds under salt stress conditions;
x' ji is the average value of the measured values of the germination vigor of the alfalfa seeds under the normal culture condition;
yi is the salt damage index of the alfalfa seeds;
and (4) judging a result: the larger the Yi value is, the more the material is damaged by salt, whereas the smaller the Yi value is, the stronger the salt resistance of the material is.
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