CN106370876A - Indirect immunofluorescence automatic treatment instrument - Google Patents

Indirect immunofluorescence automatic treatment instrument Download PDF

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Publication number
CN106370876A
CN106370876A CN201610971307.5A CN201610971307A CN106370876A CN 106370876 A CN106370876 A CN 106370876A CN 201610971307 A CN201610971307 A CN 201610971307A CN 106370876 A CN106370876 A CN 106370876A
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fixedly connected
end surface
top end
sample
sucker
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CN201610971307.5A
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CN106370876B (en
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许兴路
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/025Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

Provided is an indirect immunofluorescence automatic treatment instrument which belongs to the technical field of medical equipment. The instrument includes a fixed base and a fixed bracket. The instrument is characterized in that: the center of the front edge of the fixed base upper surface is placed with a sample acceptance rail, the fixed base lower surface is connected with the fixed bracket, the right side of the fixed bracket front surface is connected with a vacuum suction device. A stroma lamella storage, sealing and cleaning device is located under the vacuum suction device which is also connected with the fixed base. The left side of the fixed bracket is connected with a sample feeding needle device. The center of the fixed base rear surface is connected with an incubation disk device. The inside of the incubation disk device is connected with a sample dilution device. The left side of the incubation disk device on the fixed base is a sample feeding plate cleaning device. The center and right side in front of the fixed base upper surface are all connected with barcode scanning devices respectively. This configuration has the advantages of enhancing the completeness and correctness of immunofluorescence test, reducing the differences between batches, and alleviating the work intensity of testers.

Description

A kind of indirect immunofluorescence automatic business processing instrument
Technical field:
The invention belongs to technical field of medical instruments is and in particular to a kind of indirect immunofluorescence automatic business processing instrument.
Background technology:
In long-term clinical practice, during long-term clinical experiment, it is glimmering that docimaster does immunity using manual method Light is tested, and manual experiment has artifical influence factor greatly, and large sample amount tests response time irregular one, cleaning cylinder cross-contamination etc. Shortcoming, needs automatization to realize the instrument of immunofluorescence experiment, and current tool is all more or less changed former with the instrument of this function Promising manual experiment and the immunofluorescence experiment method that designs, will necessarily affect experiment effect, and all solve very well from The problem of dynamicization mounting.
Content of the invention:
The purpose of the present invention is: in order to help Clinical Laboratory teacher to complete immunofluorescence experiment automation mechanized operation, is ensureing handss On the premise of work experiment effect, improve the safety of fluorescence immunoassay experiment, accuracy, reliability, reduce differences between batches, liberation Docimaster labour force, improves work efficiency, and its technical scheme is as follows:
A kind of indirect immunofluorescence automatic business processing instrument, it includes firm banking 1, sample introduction track 2, fixed support 3, sucker Device 4, substrate tablet storage mounting cleaning device 5, sample-adding needle device 6, incubation disk device 7, Sample Dilution device 8, sample-adding plate are clear Cleaning device 9, bar code scanner 10 it is characterised in that: on described firm banking 1, top end surface front edge middle is fixing even It is connected to sample introduction track 2, on described firm banking 1, top end surface is fixedly connected with fixed support 3, the front top of described fixed support 3 On the right side of surface, place is fixedly connected with Acetabula device 4, and described 4 times top tables of Acetabula device are provided with substrate tablet storage mounting cleaning device 5 and be fixedly connected with firm banking 1, the left top end surface of described fixed support 3 is fixedly connected with sample sample-adding needle device 6, described solid Determine top end surface rear middle on base 1 and be fixedly connected with incubation disk device 7, top end surface circle on described incubation disk device 7 It is fixedly connected with Sample Dilution device 8 inside at the heart, described firm banking 1 is fixedly connected with sample-adding at top end surface left hand edge Plate cleaning device 9, is respectively fixedly connected with bar code scanner at middle and right side in front of top end surface on described firm banking 1 10, described Acetabula device 4 is fixedly connected on institute by sucker fixed plate 4-1, sucker slide block 4-2, sucker 4-3, sucker fixed plate 4-1 State fixed support 3 front surface, before described sucker fixed plate 4-1, top end surface is fixedly connected with sucker slide block 4-2, described sucker is slided Under block 4-2, top end surface is symmetrical is respectively fixedly connected with sucker (4-3), described sucker fixed plate 4-1 and described fixed support 3 It is connected with each other by slideway with described sucker fixed plate 4-1 with described sucker slide block 4-2, and described slideway is connected with motor, two Under described sucker 4-3, not at grade, described sample-adding needle device 6 is made up of top end surface portable plate and sampling probe, described Portable plate is connected with each other by slideway with described portable plate with described fixed support 3 and described sampling probe, and described slideway is connected with Motor, described incubation disk device 7 is made up of ring gear 7-1, support 7-2, annular incubation disk 7-3, sample-adding plate 7-4, described solid Determine top end surface on base 1 and be connected with ring gear 7-1, top end surface edge average mark on described ring gear 7-1 Cloth is fixedly connected with several supports 7-2, and on described support 7-2, top end surface is fixedly connected with annular incubation disk 7-3, described ring Under shape incubation disk 7-3, top end surface edge is evenly distributed and is fixedly connected with heating film, and described annular incubation disk 7-3 pushes up End surfaces edge is evenly distributed and is fixedly connected with several and is loaded plate 7-4, described sample-adding plate 7-4 about outside top end surface Respectively it is fixedly connected with fixed block, under described fixed block, top end surface is fixedly connected on described annular incubation disk 7-3 in the middle of edge Surface, on the right side of described fixed block, top end surface outer ledge is fixedly connected with gear, and the top end surface center of circle inside described gear Place is fixedly connected with rotating shaft, and described rotating shaft is penetrated described fixed block and is fixedly connected with described sample-adding plate 7-4, sample-adding plate cleaning dress Put 9 to be stepped shape, and in described sample-adding plate cleaning device 9, top end surface front edge is provided with several inside middle Cleaning head, described sample-adding plate cleaning device 9 right side top end surface is fixedly connected with slide rail, and described slide rail outer tip end surface is fixed It is connected with tooth bar, at described gear lower surface, is provided with eccentric, described eccentric and described slide rail are respectively fixedly connected with motor, institute State and be respectively fitted with sample loading and unloading device inside top end surface left and right edges on sample introduction track 2, and described sample introduction track 2 upper surface is provided with Under propeller, and described propeller, top end surface is fixedly connected with slide rail, and described slide rail lower surface is fixedly connected with motor, and Described slide rail is fixedly connected with described firm banking 1 lower surface, if being placed with inside described substrate tablet storage mounting cleaning device 5 Dry individual substrate tablet, coverslip and mounting seal, and described substrate tablet number is relative with described coverslip quantity and size with size Should, it is placed with rinse bath in the described substrate tablet storage one of lattice of mounting cleaning device 5, and described substrate tablet storage mounting is clear It is placed with mounting liquid, described substrate tablet storage 5 times top end surfaces of mounting cleaning device are fixedly connected in the one of lattice of cleaning device 5 There is microscope video collector, on described firm banking 1, top end surface is fixedly connected with Sample Dilution device 8, and described sample On air mix facilities 8, the symmetrical each fixation of top end surface circle centre position is fitted with several dilution traps, and described dilution groove edge is put down All it is distributed with several fluorescent liquid test tube jacks, top end surface and described annular incubation disk 7-3 on described Sample Dilution device 8 At grade, and described Sample Dilution device 8 and described annular incubation disk 7-3 are concentric circular to upper top end surface, and described Sample Dilution device 8 is mutually permanently connected by motor with described firm banking 1.
The invention has the benefit that completing immunofluorescence experiment to solve Clinical Laboratory teacher, improve fluorescence immunoassay real The completeness tested, accuracy, reliability, reduces differences between batches, liberates docimaster labour force, improve work efficiency.
Brief description:
Fig. 1 is the overall structure diagram of the present invention;
Fig. 2 is the enlarged diagram of Acetabula device 4 of the present invention;
Fig. 3 is the enlarged diagram of incubation disk device 7 of the present invention.
Specific embodiment:
With reference to a kind of indirect immunofluorescence automatic business processing instrument described in Fig. 1 Fig. 2 Fig. 3, it includes firm banking 1, sample introduction rail Road 2, fixed support 3, Acetabula device 4, substrate tablet storage mounting cleaning device 5, sample-adding needle device 6, incubation disk device 7, sample Air mix facilities 8, sample-adding plate cleaning device 9, bar code scanner 10 it is characterised in that: before top end surface on described firm banking 1 Method, edge middle is fixedly connected with sample introduction track 2, and on described firm banking 1, top end surface is fixedly connected with fixed support 3, institute On the right side of top end surface before stating fixed support 3, place is fixedly connected with Acetabula device 4, and described 4 times top tables of Acetabula device are provided with substrate Piece storage mounting cleaning device 5 is simultaneously fixedly connected with firm banking 1, and the left top end surface of described fixed support 3 is fixedly connected with sample Sample-adding needle device 6, on described firm banking 1, top end surface rear middle is fixedly connected with incubation disk device 7, described incubation disk It is fixedly connected with Sample Dilution device 8, the top end surface left side on described firm banking 1 inside top end surface circle centre position on device 7 It is fixedly connected with sample-adding plate cleaning device 9, each fixation at middle and right side in front of top end surface on described firm banking 1 at edge It is connected with bar code scanner 10, by sucker fixed plate 4-1, sucker slide block 4-2, sucker 4-3, sucker is solid for described Acetabula device 4 Fixed board 4-1 is fixedly connected on described fixed support 3 front surface, and before described sucker fixed plate 4-1, top end surface is fixedly connected with suction Disk slide block 4-2, under described sucker slide block 4-2, top end surface is symmetrical is respectively fixedly connected with sucker (4-3), and described sucker is fixed Plate 4-1 is connected with each other by slideway with described sucker fixed plate 4-1 with described fixed support 3 and described sucker slide block 4-2, and described Slideway is connected with motor, and under two described sucker 4-3, not at grade, described sample-adding needle device 6 is by activity for top end surface Plate and sampling probe composition, described portable plate is interconnected by slideway phase with described portable plate with described fixed support 3 and described sampling probe Connect, and described slideway be connected with motor, described incubation disk device 7 by ring gear 7-1, support 7-2, annular incubation disk 7-3, plus Model 7-4 forms, and on described firm banking 1, top end surface is connected with ring gear 7-1, top table on described ring gear 7-1 Face edge is evenly distributed and is fixedly connected with several supports 7-2, and on described support 7-2, top end surface is fixedly connected with ring Shape incubation disk 7-3, under described annular incubation disk 7-3, top end surface edge is evenly distributed and is fixedly connected with heating film, institute State top end surface edge on annular incubation disk 7-3 be evenly distributed be fixedly connected with several be loaded plate 7-4, described sample-adding plate 7-4 about be respectively fixedly connected with fixed block in the middle of top end surface outer ledge, under described fixed block, top end surface is fixedly connected on Described annular incubation disk 7-3 upper surface, on the right side of described fixed block, top end surface outer ledge is fixedly connected with gear, and described tooth Wheel inner side top end surface circle centre position is fixedly connected with rotating shaft, and described rotating shaft penetrates described fixed block with described sample-adding plate 7-4 admittedly Fixed connect, sample-adding plate cleaning device 9 be stepped in shape, and described sample-adding plate cleaning device 9 top end surface front edge from It is provided with several cleaning heads, described sample-adding plate cleaning device 9 right side top end surface is fixedly connected with slide rail, described inside middle Slide rail outer tip end surface is fixedly connected with tooth bar, is provided with eccentric at described gear lower surface, described eccentric and described cunning Rail is respectively fixedly connected with motor, and described sample introduction track 2 is respectively fitted with sample loading and unloading device inside top end surface left and right edges, and institute State sample introduction track 2 upper surface and be provided with propeller, and under described propeller, top end surface is fixedly connected with slide rail, and under described slide rail Surface is fixedly connected with motor, and described slide rail is fixedly connected with described firm banking 1 lower surface, and described substrate tablet stores mounting Be placed with several substrate tablets, coverslip and mounting seal inside cleaning device 5, and described substrate tablet number and size with described Coverslip quantity is corresponding with size, is placed with rinse bath in the described substrate tablet storage one of lattice of mounting cleaning device 5, and It is placed with mounting liquid, described substrate tablet storage mounting cleaning dress in the described substrate tablet storage one of lattice of mounting cleaning device 5 Put 5 times top end surfaces and be fixedly connected with microscope video collector, on described firm banking 1, top end surface is fixedly connected with sample On air mix facilities 8, and described Sample Dilution device 8, the symmetrical each fixation of top end surface circle centre position is fitted with several dilutions Groove, and described dilution groove edge is evenly distributed and is provided with several fluorescent liquid test tube jacks, top table on described Sample Dilution device 8 With the upper top end surface of described annular incubation disk 7-3 at grade, and described Sample Dilution device 8 and described annular are incubated in face Disk 7-3 is concentric circular, and described Sample Dilution device 8 is mutually permanently connected by motor with described firm banking 1.Firm banking 1, Sample introduction track 2, fixed support 3, Acetabula device 4, substrate tablet storage mounting cleaning device 5, sample-adding needle device 6, incubation disk device 7th, Sample Dilution device 8, sample-adding plate cleaning device 9, bar code scanner 10
During use, first several are placed in the loading and unloading device on sample introduction track 2 equipped with the test tube case of serum, then will Loading and unloading device is placed in the sample introduction track 2 on firm banking 1, then using propeller, test tube case is pushed in the middle of sample introduction track 2 Place, then the bar code on each test tube on test tube case is carried out barcode scanning by Mobile barcode scanning means 10, then promotes again Test tube case makes that test tube case is identical with fixed support 3 position in front to carry out barcode scanning by the bar code on test tube case again, then left and right Mobile sample-adding needle device 6 will be loaded needle device 6 and move to above test tube case, then move up and down the sampling on sample-adding needle device 6 Pin is then placed within serum imbibition in the dilution trap on Sample Dilution device 8, makes serum anti-with the diluent in dilution trap Should, then using sampling probe, the serum after diluting is placed in the sample-adding plate 7-4 on incubation disk device 7, then rotates annular Gear 7-1 simultaneously drives annular incubation disk 7-3 mobile to Acetabula device 4 by support 7-2, then moves left and right sucker fixed plate 4- Above this air mix facilities of 1 to setting-out 8, then moving up and down sucker slide block 4-2 makes sucker 4-3 contact with substrate tablet and adsorb, so Afterwards substrate tablet is placed on sample-adding plate 7-4 and is reacted, after reaction, again substrate tablet is moved to substrate tablet using Acetabula device 4 Rinse bath in storage mounting cleaning device 5 is interior and cleans, and then rotates incubation disk device 7 and will be placed with the sample-adding plate of serum 7-4 goes at sample-adding plate cleaning device 9, and tooth bar is moved to above gear, so by the slide rail then moving in sample-adding plate cleaning device 9 Rotating the eccentric below tooth bar afterwards makes rack and pinion contact, and then shifting sledge makes sample-adding plate 7-4 rotate and will add again Model 7-4 is placed on above the cleaning head being loaded in plate cleaning device 9 and is carried out, and after cleaning finishes, will be loaded plate 7-4 and turn To above annular incubation disk 7-3, then using sample-adding needle device 6, the fluorescent liquid in Sample Dilution device 8 is dropped in sample-adding plate 7-4 Interior, then go at dress substrate tablet storage mounting cleaning device 5 by rotating annular incubation disk 7-3, then again by the base after cleaning Matter piece covers and reflects again on sample-adding plate 7-4, is picked up mounting seal using sucker 4-3 simultaneously and mounting seal is inserted envelope Then the mounting seal speckling with mounting liquid is contacted by piece liquid with coverslip, then after the completion of reaction, with Acetabula device 4 by base Matter piece picks up and is placed on to drip to be had on the coverslip of mounting liquid, finally takes serum on substrate tablet using microscope video collector Turn over sampled images, and be transferred to and may be viewed by display screen.
Certainly, described above is not limitation of the present invention, and the present invention is also not limited to the example above, and this technology is led Change, remodeling, interpolation or replacement that the technical staff in domain is made in the essential scope of the present invention, also should belong to the present invention's Protection domain.

Claims (8)

1. a kind of indirect immunofluorescence automatic business processing instrument, including firm banking (1), sample introduction track (2), fixed support (3), inhales Disk device (4), substrate tablet storage mounting cleaning device (5), sample-adding needle device (6), incubation disk device (7), Sample Dilution device (8), sample-adding plate cleaning device 9, bar code scanner (10) it is characterised in that: in front of the upper top end surface of described firm banking (1) Edge middle is fixedly connected with sample introduction track (2), and the upper top end surface of described firm banking (1) is fixedly connected with fixed support (3), on the right side of top end surface before described fixed support (3), place is fixedly connected with Acetabula device (4), pushes up under described Acetabula device (4) End table is provided with substrate tablet storage mounting cleaning device (5) be fixedly connected with firm banking (1), the left top of described fixed support (3) End surfaces are fixedly connected with sample sample-adding needle device (6), and the upper top end surface rear middle of described firm banking (1) is fixedly connected with Incubation disk device (7), is fixedly connected with Sample Dilution device (8), on described firm banking (1) inside described incubation disk device 7 It is fixedly connected with sample-adding plate cleaning device (9), middle in front of top end surface on described firm banking 1 at top end surface left hand edge Be respectively fixedly connected with bar code scanner (10) at right side.
2. according to claim 1 a kind of indirect immunofluorescence automatic business processing instrument it is characterised in that: described Acetabula device (4) by sucker fixed plate (4-1), sucker slide block (4-2), sucker (4-3), sucker fixed plate (4-1) is fixedly connected on described solid Fixed rack (3) front surface, before described sucker fixed plate (4-1), top end surface is fixedly connected with sucker slide block (4-2), described sucker Under slide block (4-2), top end surface is symmetrical is respectively fixedly connected with sucker (4-3), and described sucker fixed plate (4-1) is solid with described Fixed rack (3) and described sucker slide block (4-2) are connected with each other by slideway with described sucker fixed plate (4-1), and described slideway is even It is connected to motor, under two described suckers (4-3) top end surface is not at grade.
3. according to claim 1 a kind of indirect immunofluorescence automatic business processing instrument it is characterised in that: described sample-adding needle device (6) be made up of portable plate and sampling probe, described portable plate and described fixed support (3) and described sampling probe and described portable plate by Slideway is connected with each other, and described slideway is connected with motor.
4. according to claim 1 a kind of indirect immunofluorescence automatic business processing instrument it is characterised in that: described incubation disk device (7) it is made up of ring gear (7-1), support (7-2), annular incubation disk (7-3), sample-adding plate (7-4), described firm banking (1) Upper top end surface is connected with ring gear (7-1), and the upper top end surface edge of described ring gear (7-1) is evenly distributed solid Surely it is connected with several supports (7-2), the upper top end surface of described support (7-2) is fixedly connected with annular incubation disk (7-3), described Under annular incubation disk (7-3), top end surface edge is evenly distributed and is fixedly connected with heating film, described annular incubation disk (7- 3) upper top end surface edge is evenly distributed and is fixedly connected with several sample-addings plate (7-4), pushes up about described sample-adding plate (7-4) Respectively it is fixedly connected with fixed block, under described fixed block, top end surface is fixedly connected in described activity in the middle of end surfaces outer ledge Ring (7-3) upper surface, on the right side of described fixed block, top end surface outer ledge is fixedly connected with gear, and top inside described gear Surface circle centre position is fixedly connected with rotating shaft, and described rotating shaft is penetrated described fixed block and is fixedly connected with described sample-adding plate (7-4).
5. according to claim 1 a kind of indirect immunofluorescence automatic business processing instrument it is characterised in that: sample-adding plate cleaning device (9) be stepped shape, and the upper top end surface front edge of described sample-adding plate cleaning device (9) be provided with inside middle some Individual cleaning head, on the right side of described sample-adding plate cleaning device (9), top end surface is fixedly connected with slide rail, described slide rail outer tip end surface It is fixedly connected with tooth bar, at described gear lower surface, is provided with eccentric, described eccentric and described slide rail are respectively fixedly connected with electricity Machine.
6. according to claim 1 a kind of indirect immunofluorescence automatic business processing instrument it is characterised in that: described sample introduction track (2) respectively it is fitted with sample loading and unloading device inside upper top end surface left and right edges, and described sample introduction track (2) upper surface is provided with propulsion Under device, and described propeller, top end surface is fixedly connected with slide rail, and described slide rail lower surface is fixedly connected with motor, and described Slide rail is fixedly connected with described firm banking (1) lower surface.
7. according to claim 1 a kind of indirect immunofluorescence automatic business processing instrument it is characterised in that: the storage of described substrate tablet It is placed with several substrate tablets, coverslip and mounting seal inside mounting cleaning device (5), and described substrate tablet number and size Corresponding with described coverslip quantity and size, described substrate tablet stores in the one of lattice of mounting cleaning device (5) and is placed with It is placed with mounting liquid, described substrate tablet storage in rinse bath, and the one of lattice of described substrate tablet storage mounting cleaning device (5) Under mounting cleaning device (5), top end surface is fixedly connected with microscope video collector.
8. according to claim 4 a kind of indirect immunofluorescence automatic business processing instrument it is characterised in that: described firm banking 1 Upper top end surface is fixedly connected with Sample Dilution device (8), and about the upper top end surface circle centre position of described Sample Dilution device (8) Symmetrically each fixation is fitted with several dilution traps, and described dilution groove edge be evenly distributed be provided with several fluorescent liquid test tubes insert Hole, on described Sample Dilution device (8), top end surface and described annular incubation disk (7-3) go up top end surface at grade, And described Sample Dilution device (8) and described annular incubation disk (7-3) are concentric circular, and described Sample Dilution device (8) and institute State firm banking (1) to be mutually permanently connected by motor.
CN201610971307.5A 2016-10-28 2016-10-28 A kind of indirect immunofluorescence automatic business processing instrument Active CN106370876B (en)

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CN109917056A (en) * 2019-04-04 2019-06-21 广东出入境检验检疫局检验检疫技术中心 A kind of liquid phase autosampler and cleaning method
CN112747990A (en) * 2020-12-23 2021-05-04 山东骏腾医疗科技有限公司 Automatic piece sealing box for tissue slice

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CN104535782A (en) * 2014-12-29 2015-04-22 基蛋生物科技股份有限公司 Full-automatic immuno-fluorescence quantitative analysis device and implementation method
CN105572407A (en) * 2016-01-27 2016-05-11 广州万孚生物技术股份有限公司 Full-automatic fluorescent quantitative immunity analyzer and detection method

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US20030032191A1 (en) * 2001-07-30 2003-02-13 Hilson Richard O. Sample processing apparatus and methods
WO2011109580A1 (en) * 2010-03-03 2011-09-09 Battelle Memorial Institute Sample preparation for spectroscopy analysis
CN104049095A (en) * 2014-06-26 2014-09-17 深圳市同盛医疗设备有限公司 Excrement detector and excrement detection method
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109917056A (en) * 2019-04-04 2019-06-21 广东出入境检验检疫局检验检疫技术中心 A kind of liquid phase autosampler and cleaning method
CN112747990A (en) * 2020-12-23 2021-05-04 山东骏腾医疗科技有限公司 Automatic piece sealing box for tissue slice

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