A kind of treat the impaired compound Chinese medicinal preparation of type 2 diabetes mellitus islet function and its preparation
Method
Technical field
The present invention relates to a kind of treat impaired compound Chinese medicinal preparation of type 2 diabetes mellitus islet function and preparation method thereof, belong to
In TCM treatment of diabetes technical field.
Background technology
Diabetes are one group due to being characterized with chronic hyperglycemia caused by insulin secretion and (or) effect defect
Metabolic disease.IDF (idf) announces sixth version " state in the United Nations's diabetes day on November 14th, 2013
Border diabetes alliance diabetes map " display, the whole world there are about 3.82 hundred million adults with diabetes within 2013, and about 5,100,000 people are dead
Die, just have 1 people to die from diabetes within averagely every 6 seconds, and expect 2035, this patient's number can rise to 5.92 hundred million, become
For endangering the principal disease of human health, wherein, type 2 diabetes mellitus (t2dm) account for the 90%~95% of diabetes total number of persons.Under the pressure of tight
High popular situation, countries in the world and international organization all give to pay high attention to the research of t2dm.
T2dm pathogenesis are extremely complex, and Western medicine is directed to different ill mechanism, and its action target spot is clearer, and effect is relatively
By force.But, type 2 diabetes mellitus patient is the entirety of organic connections, and many metabolism links of diabeticss body are deposited simultaneously
In imbalance, act on merely a certain target drug and be difficult to play optimum therapeuticing effect, bring side effect also relatively obvious, constitutional
Inefficacy, secondary failure problem are also more serious.Apply if we are in type 2 diabetes mellitus early stage and there are integrally-regulated and Mutiple Targets
Medicine certainly will improve therapeutic effect, symptom management development and the generation reducing complication.Therefore, research is integrally-regulated, many
Target treatment and the low Chinese medicine of side effect have become a direction of type 2 diabetes mellitus drug research.
The medicine for the treatment of diabetes is various in the market, and through retrieval, Chinese patent discloses following treatment diabetes to be had
The report of the Chinese medicine preparation closing, now takes passages as follows:
L, Chinese patent<application number>00108421<denomination of invention>a kind of treat medicine of diabetes and preparation method thereof<application
People>the bright Liu Ping of Korea Spro ten thousand, branch of First People's Hospital, the trend in Yangquan City Li Jia village<digest>is the invention discloses a kind of new controlling
Treat the medicine of diabetes, it is with Radix Astragali, the Radix Rehmanniae, Radix Salviae Miltiorrhizae, Rhizoma Dioscoreae, Semen Litchi, Radix Scrophulariae, Rhizoma Atractylodis, Fructus Lycii, Fructus Mume, jade
Rice palpus, Fructus Schisandrae Chinensis, Radix Trichosanthis, Pedicellus Melo, Cortex Cinnamomi, Pupa bombycises, Ramulus Euonymi, Concha Meretricis seu Cyclinae, Endothelium Corneum Gigeriae Galli, Radix Puerariae, Folium Mori, Rhizoma Polygonati are raw material,
According to the different characteristic of every taste Chinese medicine respectively through lyophilizing, dry, calcine, dedusting, pulverizing, prepare in proportion, then through ripening, go out
Powder is made after bacterium.
2nd, Chinese patent<application number>99120424<denomination of invention>a kind of treat medicine of diabetes and preparation method thereof<
Applicant>Shao Yujun<digest>the invention discloses a kind of treat Chinese patent medicine of diabetes and preparation method thereof, this Chinese patent medicine
By Pollen Pini, Endothelium Corneum Gigeriae Galli, the Radix Astragali, Radix Pseudostellariae, Cortex Phellodendri, Stigma Maydis, the Rhizoma Anemarrhenae, Radix Rehmanniae, Rhizoma Polygonati, Cortex Mori, Rhizoma Dioscoreae, Semen Litchi, pellet
Spice in ginseng, Fructus Corni, Radix Et Rhizoma Rhei and Fructus Schisandrae Chinensis 16 taste, is divided into four groups according to its effective ingredient and physicochemical property, is respectively adopted water
The technique decocted, be directly used as medicine after water decoction-alcohol sedimentation, ethanol extraction and pulverizing, is prepared into capsule, treats diabetes.
The problem that prior art exists is least height, and medicine forms numerous and diverse, high cost, and composition is wayward, secondary work
With many deficiencies such as larger.
Content of the invention
It is an object of the invention to provide a kind of treat the impaired compound Chinese medicinal preparation of type 2 diabetes mellitus islet function and its preparation
Method, medicament composing prescription rationally, using safe, evident in efficacy, Small side effects, moderate cost, easy to make, medicine source is enriched, take
Convenient, have and reduce the blood glucose of type 2 diabetes mellitus patient, blood fat, the effect of blood viscosity, solve that background technology exists above-mentioned asking
Topic.
The object of the invention is achieved through the following technical solutions:
A kind of treat the impaired compound Chinese medicinal preparation of type 2 diabetes mellitus islet function, this compound Chinese medicinal preparation is by following weight parts
Material medicine make:
10~40 parts of Rhizoma Curcumae Longae, 10~40 parts of Radix Et Rhizoma Rhei, 5~30 parts of Rhizoma Alismatis, 5~30 parts of Fructus Ligustri Lucidi, 5~30 parts of Semen Vitis viniferae, Semen Lini 5
~30 parts.
A kind of preparation method treating the impaired compound Chinese medicinal preparation of type 2 diabetes mellitus islet function, the method is water extraction,
Comprise the steps:
A. weigh material medicine: 10~40 parts of Rhizoma Curcumae Longae according to weight portion, 10~40 parts of Radix Et Rhizoma Rhei, 5~30 parts of Rhizoma Alismatis, Fructus Ligustri Lucidi 5~
30 parts, 5~30 parts of Semen Vitis viniferae, 5~30 parts of Semen Lini;Add water to cook twice, by weight, the addition decocting water every time is
8 times of material medicine total weight parts, decoct 2 hours every time, filter, merging filtrate, are concentrated into extractum;
B. dry, pulverize into fine powder as active component;
C. the active component described in step b is made various dosage forms together with the carrier pharmaceutically accepting or excipient.
A kind of preparation method treating the impaired compound Chinese medicinal preparation of type 2 diabetes mellitus islet function, the method is water extraction,
Comprise the steps:
A. weigh material medicine: 10~40 parts of Rhizoma Curcumae Longae according to weight portion, 10~40 parts of Radix Et Rhizoma Rhei, 5~30 parts of Rhizoma Alismatis, Fructus Ligustri Lucidi 5~
30 parts, 5~30 parts of Semen Vitis viniferae, 5~30 parts of Semen Lini;Add water to cook twice, by weight, the addition decocting water every time is
8 times of material medicine total weight parts, decoct 2 hours every time, filter, merging filtrate, are concentrated into extractum, as active component;
B. the active component described in step a is made various dosage forms together with the carrier pharmaceutically accepting or excipient.
We find in t2dm research for many years, how this disease is in middle aged sequela, and overweight people is in the majority, fertile with surfeit
It is inducement that sweet, sitting is moved etc. less, and meet " vigour be unequal to its people of essence derived from food is fertile and not longevity " viewpoint and delicious food savoury must not activating QI and
Raw phlegm-damp fat is turbid to stop up long-pending internal basic characteristics.Based on this it is considered that the turbid heap soil or fertilizer over and around the roots of fat is long-pending, strongly fragrant longization is hot is the main of type 2 diabetes mellitus
Pathomechanism, therefore draft with heat clearing away, let out the blood sugar-reduction formula as primary efficacy for the turbid, blood stasis dispelling, side is by Rhizoma Curcumae Longae, Radix Et Rhizoma Rhei, Rhizoma Alismatis, Fructus Ligustri Lucidi entirely
Son, Semen Lini, Semen Vitis viniferae composition, all medicines share makes the hot and suffocating of lung be discongested, can lead to the turbid pathogen dropping clear key, in clinical practice again
Obtain preferable hypoglycemic effect.
In medical research, from Chinese medical theory, through infrastest and with reference to clinical experience it is believed that diabetes are normal
See that pathogenesis is: fat is turbid to stop up long-pending, strongly fragrant longization heat, and disease has blood stasis (blood stasis) long concurrently.It is polydipsia, many according to diabeticss clinical manifestation
Food, polyuria, become thin, the symptom such as spiritlessness and weakness, founded heat clearing away, let out turbid, blood stasis dispelling therapeutic rules, in the guidance of this therapeutic rules
Under prescription in addition.
Every effect of material medicine and its effect in prescription simply of the present invention are described below:
1st, Rhizoma Curcumae Longae: [source] this product is the dry rhizome of zingiberaceous plant Rhizoma Curcumae Longae curcuma longa l..[nature and flavor] are pungent, bitter, temperature.
[return through] returns spleen, Liver Channel.[chemical composition] Rhizoma Curcumae Longae contains volatile oil 4.5%, 6%.Turmerone 58%, zingerene is contained in volatile oil
25%th, phellandrene 1%, 1, 8-Cineole 1%, sabinene 0.5%, Borneolum Syntheticum 0.5%, dehydroturmerone etc..Also contain curcumin
0.3%th, 1.1%, 4.8% and arabinose 1.1%, Fructose 12%, glucose 28%, fatty oil, starch, oxalates etc..[work(
Can cure mainly] blood-breaking, inducing menstruation to relieve menalgia.Twinge for chest side of body, amenorrhea, abdominal mass, rheumatism takes on arm pain, tumbling and swelling.
2nd, Radix Et Rhizoma Rhei: [source] this product is polygonum rheum palmatum rheum palmatum l.Rheum tanguticum rheum
tanguticum maxim. ex balf.Or Rheum officinale rheum officinale baill.Root and rhizome is dried.[property
Taste] bitter, tremble with fear.[return through] returns spleen, stomach, large intestine, liver, pericardium channel.The root shape of [chemical composition] sorrel, Radix Et Rhizoma Rhei and rheum tanguticum Tschircs
Contain anthraquinone analog compound about 3% in stem and root, include free chrysophanol (chrysophanol) with bonding state, chrysophanic acid
(rhein), aloe-emodin (aloe-emodin), rheum emodin (emodin), parietin (parietin, c22h22o10),
Physcione (emodinmonomethyl ether), under its main rushing down, composition is associativity chrysophanic acid anthrone-Sennosides
A. b. c(sennoside a. b. c) wherein Sennosides a(sennoside a) it is principle active component.Additionally, still containing tan
Matter about 5% and free gallic acid, cinnamic acid and its lipid etc..Leaf contains quercitrin, and only the leaf of sorrel is with hyperin
(hyperoside) content is most.[indication] purging heat and dredging bowels, removing pathogenic heat from blood and toxic substance from the body, eliminating blood stasis and inducing menstruation.For excess-heat constipation, stagnant abdomen
Bitterly, dysentery is not well, jaundice due to damp-heat, blood-head tells nosebleed, conjunctival congestion, pharyngeal swelling, abdominalgia with intestinal abscess, carbuncle swells furunculosis, blood stasis closed, traumatic injury,
External treatment burn due to hot liquid or fire;Upper gastrointestinal hemorrhage.Radix et Rhizoma Rhei (stir-fried with wine) is apt to clear part of the body cavity above the diaphragm housing the heart and lungs noxious heat in the blood system.For conjunctival congestion pharyngeal swelling, gingivitises.Radix Et Rhizoma Rhei
Rush down and exert oneself to delay, eliminating fire and detoxication.For pathogenic fire,toxin and furuncles.Charred Radix Et Rhizoma Rhei cooling blood and removing stasis stop blooding.There is stasis of blood hemorrhage for heat in blood.
3rd, Rhizoma Alismatis: [source] this product is the drying of Notes On Alism At Aceae Rhizoma Alismatis alisma orientalis (sam.) juzep.
Tuber.[nature and flavor] are sweet, tremble with fear.[return through] returns kidney, bladder warp.Five kinds of triterpeness are separated in [chemical composition] [chemical composition] tuber
Compound: alisol a, alisol b, acetic acid alisol a ester, acetic acid alisol b ester and table alisol a;Separately (include bran containing volatile oil
Aldehyde), a small amount of alkaloid, asparagine, plant sterols, a kind of phytosterolin, fatty acid (Palmic acid, stearic acid, oil
Acid, linoleic acid);Also resinous, protein and volume starch (23%).[indication] diuresis, clearing away damp-heat.For urine not
Profit, oedema turgor, oliguria of having loose bowels, dizziness due to fluid-retention, puckery pain of heat gonorrhea;Hyperlipidemia.
4th, Fructus Ligustri Lucidi: [source] this product is being dried to of Oleaceae plants Fructus Ligustri Lucidi ligustrum lucidum ait.
Ripe fruit.[nature and flavor] sweet, bitter, cold.[return through] returns liver and kidney channel.[chemical analysis] contain nuzhenide (nuzhenide), foreign olive
Olive hardship glycoside (oleuropein), oleanolic acid (oleanolic acid), 4- hydroxyl-b- phenethyl-b-d- glycoside, birch
Alcohol (betulin) etc..[indication] nourishing the liver and kidney, improving eyesight black hair.For vertigo and tinnitus, soreness of the waist and knees, early whitening of beard and hair, mesh
Secretly fail to understand.
5th, Semen Vitis viniferae: [source] this product is the seed of vitaceae Fructus Vitis viniferae vitis vinifera l..[nature and flavor] sweet in the mouth
Micro- acid, mild-natured.[return through] enter liver, kidney, lung meridian.[chemical analysis] Polyphenols: contain polyphenols (gps) in Semen Vitis viniferae, main
Catechin to be had and procyanidin class.Catechin compounds include catechin, epicatechin and its epicatechol gallate, are Portugals
Main single aggressiveness in grape seed, is also procyanidin oligomer and polymeric component unit.Oils: contain rich in Semen Vitis viniferae
Rich oils and fatss, account for the 12%~15% of its weight, contain substantial amounts of unsaturated fatty acid in oil, and wherein linoleic content exists
Between 58%~78%.Volatile ingredient: also contain a small amount of volatile ingredient in Semen Vitis viniferae, these materials belong to alcohol, phenol, terpene mostly
Class material, all has higher biological activity.Other compositions: in Semen Vitis viniferae in addition to containing above-mentioned many kinds of substance, also contain thick
Albumen, aminoacid and vitamin a, e, d, k, p and various trace elements, such as calcium, zinc, ferrum, magnesium, copper, potassium, sodium, manganese, cobalt etc..[work(
Can cure mainly] there is the effect of invigorating the liver and kidney, replenishing QI and blood, nourishing the stomach to promote the production of body fluid, diuresis.
6th, Semen Lini: [source] is the root of Semen Lini section plant Semen Lini linum usitatissimum l., stem, leaf.
[nature and flavor] acrid in the mouth;Sweet;Mild-natured.[return through] liver;Stomach;Large intestine channel.[chemical composition] leaf, stem contain flavonoid glycoside: orientin
(orientin), isorientin (isoorientin), vitexin (vitexin), isovitexin (isovitexin), light Herba Viticis Cannabifoliae
Plain (lucenin) i, 6-c- xylosyl -8-c- glucosyl group apigenin (vicenin-), 6,8- bis--c- glucosyl group celerys
Dish element (vicenin).[indication] suppressing the hyperactive liver;Promoting blood circulation.Main liver wind headache;Traumatic injury;Carbuncle swells furunculosis.
The combinations thereof medicine of the present invention can be used as the formula of Chinese traditional treatment diabetes (diabetes), and its side solves and function
Cure mainly: we in Rhizoma Curcumae Longae be monarch drug, abnormal smells from the patient work hard, Great Cold is nontoxic, circulation of qi promoting dissipate strongly fragrant, can enter the heart spleen two ward off epidemic disease, promoting blood circulationization through making contributions
The stasis of blood, circulation of qi promoting is soothing the liver, inducing menstruation to relieve menalgia, has antiinflammatory, antioxidative activity;Radix Et Rhizoma Rhei is ministerial drug, bitter in the mouth, and Great Cold is nontoxic, passes through up and down,
Cover high sun very, non-this do not press down, bitter can pathogenic fire purging, bitter can qi-restoratives, at one stroke and two it.Rhizoma Curcumae Longae, Radix Et Rhizoma Rhei, the turbid yin in fall the moon.One
Rise a fall, inside and outside logical and, and pernicious influences of miscellaneous QI is paused and is disappeared.Fructus Ligustri Lucidi replenishing kidney-YIN, nourishing the liver to improve visual acuity, settling five organs, support in spirit solution
The quenching one's thirst as adjuvant drug of heat.Rhizoma Alismatis, Semen Lini, Fructus Vitis viniferae strengthening the spleen and stomach, QI invigorating, the strong will of times power, moisturize, dredging water passages, make turbid damp phlegm retention
Go out from urine, for making medicine.All medicines share makes the hot and suffocating of lung be discongested, and can lead to the turbid pathogen dropping clear key again.
The Chinese medicine preparation of the present invention is taken for type 2 diabetes mellitus patient's decoct soup, is more suitable for modern pharmaceutical enterprise and extracts effectively to become
Point, make any dosage form on the pharmaceuticss such as granule, capsule, oral liquid, because flavonoid glycoside is soluble in water, have very strong
Hypoglycemic activity, alkaloid is soluble in water to have notable hypoglycemic activity;Polysaccharide is soluble in water, has stronger blood sugar lowering and makees
With glycoside is typically dissolved in water, and saponin is soluble in hot water, can promote insulin releasing, adjusts metabolism of blood glucose;Polypeptide is typically solvable
Yu Shui.
The present invention has a characteristic that 1. the present invention is under guidance of traditional Chinese medicine theory, after animal experiments prove that, preferably goes up
State Chinese medicine, in addition scientific compatibility, making, so that each medicine is cooperated, learn from other's strong points to offset one's weaknesses, synergism, all medicines share, specimen and
Control, heighten the effect of a treatment.2. the raw materials used small toxicity of the present invention, overcomes the toxic and side effects of other hypoglycemic medicines particularly Western medicine, and
The shortcoming of the infringement to Liver and kidney after its life-time service Western medicine.The use of product of the present invention is safe by daily dose and the course for the treatment of.This product
Product are compared with metformin, and its blood sugar reducing function is suitable with metformin effect, do not have toxic and side effects compared with Western medicine.3. warp
Zoopery and clinical verification product of the present invention can make type 2 diabetes mellitus patient's fasting glucose and 2 hours blood glucoses reduction after the meal, and
Hold time longer, the person's cholesterol that simultaneously also makes hyperlipidemia and triglyceride reduce, evident in efficacy.4. scientific formula, raw material is easy
, generation technique is advanced, simple and easy to do, workable, moderate product price, suitable preparation of industrialization.5. in preparation process
According to the active ingredient of each Chinese medicine, property and formulation requirements, using water extraction, physical sterilization method, thus product effective ingredient
Content is high, stable, is unlikely to deteriorate, is conducive to preserving for a long time.
The invention has the advantages that: medicament composing prescription is reasonable, using safe, evident in efficacy, Small side effects, moderate cost, making
Convenient, medicine source is enriched, taking convenience, and raw material is easy to get, and generation technique is advanced, workable.
Specific embodiment
With reference to embodiment, the invention will be further described.
A kind of treat the impaired compound Chinese medicinal preparation of type 2 diabetes mellitus islet function, this compound Chinese medicinal preparation is by following heavy
The material medicine of amount part is made:
10~40 parts of Rhizoma Curcumae Longae, 10~40 parts of Radix Et Rhizoma Rhei, 5~30 parts of Rhizoma Alismatis, 5~30 parts of Fructus Ligustri Lucidi, 5~30 parts of Semen Vitis viniferae, Semen Lini 5
~30 parts.
A kind of preparation method treating the impaired compound Chinese medicinal preparation of type 2 diabetes mellitus islet function, the method is water extraction,
There are two kinds of preparation methoies:
The first preparation method comprises the steps:
A. weigh material medicine: 10~40 parts of Rhizoma Curcumae Longae according to weight portion, 10~40 parts of Radix Et Rhizoma Rhei, 5~30 parts of Rhizoma Alismatis, Fructus Ligustri Lucidi 5~
30 parts, 5~30 parts of Semen Vitis viniferae, 5~30 parts of Semen Lini;Add water to cook twice, by weight, the addition decocting water every time is
8 times of material medicine total weight parts, decoct 2 hours every time, filter, merging filtrate, are concentrated into extractum;
B. dry, pulverize into fine powder as active component;
C. the active component described in step b is made various dosage forms together with the carrier pharmaceutically accepting or excipient.
Second preparation method comprises the steps:
A. weigh material medicine: 10~40 parts of Rhizoma Curcumae Longae according to weight portion, 10~40 parts of Radix Et Rhizoma Rhei, 5~30 parts of Rhizoma Alismatis, Fructus Ligustri Lucidi 5~
30 parts, 5~30 parts of Semen Vitis viniferae, 5~30 parts of Semen Lini;Add water to cook twice, by weight, the addition decocting water every time is
8 times of material medicine total weight parts, decoct 2 hours every time, filter, merging filtrate, are concentrated into extractum;
B. the active component described in step a is made various dosage forms together with the carrier pharmaceutically accepting or excipient.
Specific embodiment:
Embodiment l: the preparation of granule
Weigh material medicine: 20 parts of Rhizoma Curcumae Longae, 20 parts of Radix Et Rhizoma Rhei, 15 parts of Rhizoma Alismatis, 15 parts of Fructus Ligustri Lucidi, Semen Vitis viniferae 15 according to following weight portion
Part, 15 parts of Semen Lini.
Above-mentioned raw materials medicament mixed is added water to cook twice, the addition of water is 8 times of material medicine total weight parts, every time
Decoct 2 hours, decoction liquor filters, filtrate merges, stand 12 hours, take supernatant concentration to relative density to be 1. 20-1.30
(50-70 DEG C), is mixed as extract powder with above-mentioned 200 mesh impalpable powders, takes extract powder: dextrin: starch: Microcrystalline Cellulose one 5:1:2:
2, mix, pelletize, be dried, make granule, obtaining final product name of product is hypoglycemic granule.
Embodiment 2: the preparation of granule
Weigh material medicine: 20 parts of Rhizoma Curcumae Longae, 20 parts of Radix Et Rhizoma Rhei, 15 parts of Rhizoma Alismatis, 15 parts of Fructus Ligustri Lucidi, Semen Vitis viniferae 15 according to following weight portion
Part, 15 parts of Semen Lini.
Above-mentioned raw materials medicament mixed is added water to cook twice, the addition of water is 8 times of material medicine total weight parts, every time
Decoct 2 hours, decoction liquor is filtered. merging filtrate, it is concentrated into extractum, dry, pulverize into 200 mesh fine powders;Add appropriate auxiliary
Material starch, mixes, and prepared name of product is hypoglycemic granule.
Embodiment 3: the preparation of capsule
Weigh material medicine: 20 parts of Rhizoma Curcumae Longae, 20 parts of Radix Et Rhizoma Rhei, 15 parts of Rhizoma Alismatis, 15 parts of Fructus Ligustri Lucidi, Semen Vitis viniferae 15 according to following weight portion
Part, 15 parts of Semen Lini.
Above-mentioned raw materials medicament mixed is added water to cook twice, the addition of water is 8 times of material medicine total weight parts, every time
Decoct 2 hours, filter, filtrate merges, stand 12 hours, take supernatant concentration to being dried in right amount, load capsule, obtain final product product
Entitled Jiangtang capsule.
Embodiment 4: the preparation of oral liquid
Weigh material medicine: 20 kilograms of Rhizoma Curcumae Longae according to weight meter (every weight portion be l kilogram), 20 kilograms of Radix Et Rhizoma Rhei, 15 kilograms of Rhizoma Alismatis,
15 kilograms of Fructus Ligustri Lucidi, 15 kilograms of Semen Vitis viniferae, 15 kilograms of Semen Lini.
Material medicine mixing and water adding is decocted twice, adds 8 times amount water every time, decocting the weight adding water every time is material medicine
8 times of gross weight, decoct 2 hours every time, filter, merging filtrate, stand 12 hours, take supernatant concentration to appropriate, and upper
State impalpable powder mixing, add flavoring agent, Preservative qs, mix, making name of product is oral liquid for decreasing blood sugar.
Embodiment 5: the preparation of pill
Weigh material medicine: 20 kilograms of Rhizoma Curcumae Longae according to weight meter (every weight portion be l kilogram), 20 kilograms of Radix Et Rhizoma Rhei, 15 kilograms of Rhizoma Alismatis,
15 kilograms of Fructus Ligustri Lucidi, 15 kilograms of Semen Vitis viniferae, 15 kilograms of Semen Lini.
Material medicine mixing and water adding is decocted twice, decocts 8 times that the weight adding water is material medicine gross weight every time.Often
Secondary decoction 2 hours, filters, merging filtrate, is concentrated into extractum, dry, pulverize into fine powder;Add appropriate all enter starch etc. auxiliary
Material, mixes, and water is general to be used Pulvis Fumi Carbonisatuss coating, hit light for ball, is dried, and obtaining final product name of product is Jiangtangwan pill.
Excipient in preparation method of the present invention, the addition of adjuvant, according to the concrete condition in preparation process, flexibly slap
Hold, that is, " appropriate " described in literary composition.
Instructions of taking: granule, three times a day, and one bag every time, 10 grams every bag;Capsule, three times a day, 3 every time, every
0. 35 grams;Oral liquid, three times a day, each l0ml;Pill: three times a day, 30 every time, 0.1 gram every.Before meals or taking medicine after meal
With.Note: child or hypoglycemia person without or use with caution.
The present invention proves through North China central laboratory of the college of traditional Chinese medicine of Polytechnics acute toxicity testing, this product acute oral poison
Property experiment display no overt toxicity.
Pharmacological evaluation of the present invention is as follows:
The impact to type 2 diabetes mellitus rat blood sugar for the hypoglycemic granule
(1) materials and methods
(1) positive control drug double benefit hypoglycemic granule: provided by Lunan Pharmaceutical Co., Ltd..(authentication code: traditional Chinese medicines ≯ Cui word
210950075)
(2) animal wistar rat, is purchased from China Concord Medical Science University of Chinese Academy of Medical Sciences institute of lab animals Bei Jingkang
Blue Bioisystech Co., Ltd, spf level, animal quality certification number: scxk<capital>2009-0004, body weight 120 ± 209.It is hero
Property.
(3) reagent streptozotocin is provided by Beijing universal love port commerce trading center.
(4) method takes 70 wistar rats, is randomly divided into 7 groups, i.e. blank control group (saline), model group, Western medicine pair
According to group, Chinese medicine matched group, this medicine high dose group, middle dose group, low dose group.Weigh weekly 1 time, and accordingly adjustment dispensing
Amount, is used in conjunction 4 weeks, and activities in rats situation observed to saline and at any time by matched group list, in administration 4 weeks, takes a blood sample every group of mensure from tail point
Blood glucose of rat etc., put to death administration group matched group all animals in 4 weeks, and takes main organs, sent pathological tissue room to check.
(2) result
(1) the impact administration group rat to animal ordinary circumstance is compared with blank control group, ordinary circumstance Non Apparent Abnormality.
(2) blood glucose inspection result medicine of the present invention post-prandial glycemia and fasting glucose compared with model group significantly reduce (p <
0.05), compared with melbine group, double benefit hypoglycemic granule group post-prandial glycemia and fasting glucose there was no significant difference (p > 0.05)
(table 1).
The test impact of each material medicine in the present invention:
1st, the impact to type 2 diabetes mellitus rat kidney and pathology of livers for the curcumin
Method: it is blank control group that 100 Male Wistar Rats are randomly divided into two groups: 10 rats, and 90 rats are
T2dm modeling group, using low dose of streptozotocin (30mg/kg bw) joint high glucose and high fat feedstuff induction t2dm model group.Mould
After type induction success, meet the rat 39 of t2dm standard, be randomly divided into 4 groups: t2dm model control group, curcumin low dose group
(50 mg/kg bw), curcumin middle dose group (150 mg/kg bw) and curcumin high dose group (250 mg/kg bw), give
Intervene.After 45 days, randomly select 4 from each group rat and carry out pathology detection, observe the pathological changes of t2dm rat kidney and liver
Progress.Result compared with matched group, the rat blood sugar of t2dm model group, blood fat all significantly raised (p < 0.05).With t2dm
Model control group compares, and the pathological change of curcumin intervention groups t2dm rat kidney and liver all has different degrees of improvement.
Conclusion curcumin can improve the pathological change of t2dm rats'liver, nephridial tissue.
2nd, the impact to spinal cord of diabetic rats dorsal horn tnf- alpha expression for the curcumin
Method: 30 sd male rats are randomly divided into matched group (con), diabetic groups (dm) and curcumin treatment group (dm+
), cur every group 10;Prepare rat diabetes model using lumbar injection streptozotocin (stz), dm+cur group is diabetes
Model gives Rhizoma Curcumae Longae extract for treating after being successfully prepared, con group and dm group give the normal saline of equivalent simultaneously.Using elisa method
The content of detection each group Rat Spinal Dorsal tnf- alpha molecule, compares each group Rat Spinal Dorsal tnf- α using rt-pcr method
The expression of mrna.Result: successfully prepare patients with type Ⅰ DM (tidm) rat model, this model has hyperglycemia, quality mitigates, many
The features such as drink, polyphagia, polyuria, elisa testing result display con group, dm group and dm+cur group Rat Spinal Dorsal tnf- alpha molecule
Content is respectively as follows: 21 ± 6 pg/ml, 145 ± 11 pg/ml and 80 ± 7pg/ml, dm group tnf- alpha molecule content apparently higher than
Con group (p < 0.05), after giving Rhizoma Curcumae Longae extract for treating, dm+cur group tnf- α is remarkably decreased, but remains above con group (p < 0.05).
Rt-pcr testing result display dm group Rat Spinal Dorsal tnf- α mrna is higher than con group, after giving Rhizoma Curcumae Longae extract for treating, dm+cur
Group tnf- α mrna decreases compared with dm group, and this trend is consistent with elisa result.The tidm rat ridge of conclusion: stz induction
Marrow dorsal horn tnf- alpha expression increases, and Turmeric element can reduce the expression of tidm rat marrow dorsal horn tnf- α.
3rd, the experimentation to diabetes rat vascular lesion protection mechanism for the Radix Et Rhizoma Rhei
Method: prepare diabetes rat model, and give Radix Et Rhizoma Rhei intervention, after 8 weeks, take hematometry nitric oxide (no) and endothelium
Plain -1 (et-1).Take the acetylcholine (ach) that thoracic aortic ring observes different cumulative concentrations that norepinephrine (ne) is caused
Vasoconstrictive suppression ratio.Separately leave and take one section of thoracic aorta and prepare pathological section, sp method immunohistochemical staining, between observation of cell
Adhesion molecule -1 (icam-1) and the positive expression of vascular intercellular adhesion molecule-1 (vcam-1).Result: Radix Et Rhizoma Rhei group and model
Group compares the blood plasma et-1 (p < 0.05) that can substantially suppress to raise;Raise the level (p < 0.05) of no;But all do not reach normal group
Level (p < 0.05).Model group vascular ring is significantly stronger than Normal group and Radix Et Rhizoma Rhei group (p < 0.05) to the contractile response of ne,
And Radix Et Rhizoma Rhei group is also better than Normal group (p < 0.05) to the contractile response of ne.Under identical ach concentration, model group and Radix Et Rhizoma Rhei group
Thoracic aortic ring is markedly less than normal group (p < 0.05) to the diastole effect of ach, and Radix Et Rhizoma Rhei group is better than model group (p < 0.05).Greatly
Huang Zuneng substantially suppresses diabetes rat thoracic aorta icam-1 and vcam-1 expression (p < 0.05).Conclusion: Radix Et Rhizoma Rhei has reduction
Rat et-1 and the effect raising no, can protect the arterial dilation that diabetes rat endothelium relies on, and can suppress
The expression of icam-1 and vcam-1, has anti arteriosclerosis effect.
4th, the impact to bdnf, inos and oxidative stress in diabetes mellitus encephalopathy rat hippocampus for the chrysophanol
Method: the application water maze laboratory detection impact to diabetes rat model learning and memory for the chrysophanol;Application RNA isolation kit
Acetylcholine esterase (ache) in detection different experiments group Hippocampus, CAT (chat), Brain Derived Neurotrophic Factor
(bdnf), the activity of nitric oxide synthase type (inos) and oxidative stress index of correlation (cat, sod, gsh).Result Radix Et Rhizoma Rhei
Phenol improves the ability of learning and memory of diabetes rat model, enhances ache and bdnf in diabetes rat model group Hippocampus
Activity, inhibits the activity of chat, inos, cat, sod and gsh in model group Hippocampus.Conclusion chrysophanol may be by strengthening
The activity of bdnf, the function of downward inos and antioxidation approach are thus produce protective effect to diabetes mellitus encephalopathy, it is likely to become
The newtype drug of clinical treatment diabetes mellitus encephalopathy.
5th, the impact to type 2 diabetes mellitus mouse blood sugar, insulin level for the rheum emodin
Method: blood glucose all >=the spf level kkay mice 40 of 13.9 mmol/l, be randomly divided into model group (gavaging sterilized water),
Basic, normal, high dosage Effects of Emodin group [giving 12.5,25,50 mg/ (kg.d) rheum emodin gavages respectively] and Mellitus Model With Pioglitazone
Group [gives 1.95 mg/ (kg.d) gavage], successive administration 8 weeks.Each group mice fasting glucose (fpg), empty stomach islets of langerhans is measured after 8 weeks
Plain (fins), and calculate insulin sensitivity index (isi);Measure pi3-k in mice skeletal and fatty tissue with rt-pcr method
And the mrna of glut4.Result is compared with model group, basic, normal, high dosage Effects of Emodin group and Mellitus Model With Pioglitazone group mice
Fpg, fins, isi all substantially reduce (p all < 0.05), and are in dose dependent.High dose Effects of Emodin group group fins, isi
It is above Mellitus Model With Pioglitazone group (p all < 0.05).Compared with model group, basic, normal, high dosage Effects of Emodin group and pyrrole lattice arrange
The mrna of ketone treatment group mice pi3-k, glut4 substantially increases (p all < 0.05).Conclusion rheum emodin has good blood sugar lowering, fall
Low insulin level and raising insulin sensitivity effect, its mechanism may be relevant with pi3-k, glut4 gene.
6th, Rhizoma Alismatis extract is to the treatment of streptozotocin hyperglycemia mice and protective effect
Method: 1. cause diabetes animal model to mouse peritoneal injection stz 15 0mg kg-1.igrae 1.5、3.0g•kg-
1,1,2 and 5h mensure blood glucose before administration and after administration;Blood glucose and blood fat is surveyed after continuous treatment 15d.2. continuous
Igrae 1.5,3.0g kg-13d, then ipstz 10 5mg kg-1, and continual cure 3d, survey serum islet with putting method of exempting from
Element, takes pancreas to change in light Microscopic observation islet tissue.Result rae therapeutic administratp can obviously reduce stz diabetic mice
Blood glucose and triglyceride.Preventing and treating administration can substantially resist the blood glucose rise of stz induction and islet tissue changes, and can raise
Serum insulin levels.Conclusion rae has obvious treatment and protective effect to stz diabetic mice.
7th, the impact of Extract from Rhizoma Alismatis on Normal and Alloxan Model in Mice
Method: to mouse tail vein injection alloxan 90mg kg- 1, cause diabetes model.Mensure blood glucose (glu),
Triglyceride (trig), cholesterol (chol), pancreatic amylase (amy) and serum insulin (ins).Light Microscopic observation pancreas
Histological change with islets of langerhans.Result: rae can make normal mouse blood glucose substantially reduce.Treatment can make alloxan mice in 7 days
Glu and trig reduces.Alloxan mice after light Microscopic observation rae treatment, islets of langerhans keeps normal histology's form.Rae is also
Can increasing serum ins level and the amy reduction resisting model induced by alloxan.
8th, Fructus Ligustri Lucidi hypoglycemic activity
With Fructus Ligustri Lucidi water decoction 15,30g/kg to mouse stomach 10 days, the blood glucose of normal mouse can be reduced, alloxan is drawn
The diabetes mice rising has prevention and therapeutical effect, and the blood glucose rise that antiadrenergic drug or glucose can be caused.
Oleanolic Acid in Herbal Ligustrum lucidum anti-diabetic effect
Fructus Ligustri Lucidi is the traditional Chinese medicine of China, is commonly used to the diseases such as treating cancer, hepatitis, though having been reported that in terms of blood sugar lowering,
But lack system in-depth study.
8th, anti-nonenzyme glycosylation effect of Grape Seed Proanthocyanidin Extract in Diabetic Rats experimentation
Observe the impact to non-enzymatic glycation in diabetes rat body for the grape seed polyphenols (gspe), evaluate it slow to diabetes
The intervention effect of property complication (dcc).Method wistar rats by intraperitoneal injection streptozotocin (stz) 60mg/kg causes glycosuria
Disease model, is randomly divided into blank control group, aminoguanidine (150mg kg-1 d-1) treatment group, basic, normal, high 3 dosage of gspe
(50,150,450mg kg-1 d-1) treatment group, every group 12, another 12 are set to Normal group.Routine feeding took after 12 weeks
Rat blood serum and internal organs, compare each group kidney weight/body weight ratio, compare each group renal cortex nonenzymatic glycosylation end-product with fluorescence method
(ages), full automatic biochemical apparatus detection blood glucose, T-CHOL (tc) and triglyceride (tg).Result is compared with blank control group,
Gspe can significantly reduce diabetes rat kidney weight/body weight ratio (p < 001), reduces renal cortex ages (p < 001), in gspe,
High dose group and aminoguanidine treatment group act on similar;Gspe does not make significant difference to blood glucose, tg, only gspe middle dose group tc with normal
Matched group comparing difference has significance (p < 001).Conclusion gspe can suppress internal non-enzymatic glycation, is that it prevents and treats dcc
One of main mechanism.
9th, the antioxidation to diabetic mice for the Grape Seed Extract Procyanidin
Method: diabetes model is prepared with alloxan mice, by the mice after modeling success, be divided into model group, gsep low,
Middle and high 3 dosage (50,100,150 mg/kg) group, separately sets Normal group.After successive administration 28 d, on an empty stomach after 12 h, take
Whole blood and liver, measure sudismase (sod) and glutathion peroxidase (gsh-px) vigor in whole blood and hepatic tissue
And malonaldehyde (mda) content.After result orally administration mice gsep 28 d, sod in high dose group Mouse whole blood and hepatic tissue
With gsh-px activity obviously higher than model group (p < 0.05), malonaldehyde (mda) in middle and high dosage group Mouse whole blood and hepatic tissue
Content is all significantly lower than model group (equal p < 0.05).Conclusion gsep has obvious anti-oxidation protection effect to diabetic mice.
10. the research on high fat obesity-induced mice blood glucose and Glucagons like peptide-1 secretion impact for the Semen Lini seed oil
Method: 30 male c57bl/6j mices are divided into chow diet (nd), high fat diet (hfd) and Semen Lini seed oil
(fod) group, every group 10, after feeding 8 weeks, row lumbar injection glucose tolerance test (ipgtt), insulin tolerance tests
(itt) test with glp-1 secretion.Result fod group fpg, insulin level are less than hfd group (p < 0.01).Ipgtt result shows,
Nd, fod group 120min Area under the curve of blood glucose (auc) are less than hfd group (p < 0.01).Itt result shows, hfd, fod group
120min insulin auc comparing difference is not statistically significant.After waiting dosage Semen Lini seed oil to feed 30min, hfd, fod group blood
Slurry glp-1 level raises, and fod group blood plasma glp-1 level is compared with hfd group height (p < 0.01).Conclusion is on the basis of high fat diet
Supplement Semen Lini seed oil for a long time and can improve mouse blood sugar level and ir, its mechanism may be by promoting intestinal secretion glp-1 real
Existing.
The research to diabetes rat auxiliary hyperglycemic function for the 11. Semen Lini seed oil
Method: high lipid food is fed sd male rat and set up obese model;Obese model rat disposable celiac is injected 40
Mg/kg streptozotocin sets up diabetes model;Diabetic model rats are randomly divided into model control group and Semen Lini seed oil
Basic, normal, high dosage group, every group 8, rating model rat fasting blood-glucose and carbohydrate tolerance after continuous gavage 30 d.[result] Caulis et Folium Lini
Seed seed oil high dose group can reduce fasting glucose and the carbohydrate tolerance of diabetes rat.[conclusion] Semen Lini seed oil has auxiliary fall
The function of low blood glucose in diabetic rats.