CN106359659A - Composite nutrient blend oil with ulcer restoration effect, and preparation method and application thereof - Google Patents
Composite nutrient blend oil with ulcer restoration effect, and preparation method and application thereof Download PDFInfo
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- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
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Abstract
The invention discloses composite nutrient blend oil with an ulcer restoration effect, and a preparation method and application thereof. The composite nutrient blend oil is prepared from okra seed oil, fructus cannabis oil and auxiliary oil. The composite nutrient blend oil with the ulcer restoration effect provided by the invention is prepared through sieving by a plurality of tests; the composite nutrient blend oil is prepared from natural traditional medical and edible dual purpose vegetable oil with multiple health care efficacy, so that no toxic or side effects exist; health and safety are realized; experimental results show that the composite nutrient blend oil has good mucosal restoration effect and nutritive values; various ulcers such as oral mucosal ulcer, skin surface ulcer and gastric ulcer can be obviously restored. The preparation method provided by the invention has the advantages that a whole preparation process design is used; the automation degree is high; the extraction efficiency is high; the effective ingredients in raw materials can be well preserved; the impurities are few; no toxic or side effects exist.
Description
Technical field
The present invention relates to a kind of natural plants fluid composition, more particularly to a kind of compound battalion with ulcer repair
Foster mixed oil and preparation method thereof.
Background technology
Skin and mucosa, as the first line of defence of human immune system, are the important screens of body defenses alien material invasion
Barrier.When skin and mucosa due to wound, microorganism infection, tumor, circulatory disturbance and delayed ischemic neurological deficits, immunologic dysfunction or
When the reasons such as congenital skin injury occur ulcer, there is limit office property defect, fester in skin or mucous membrane surface tissue, often merge chronic
Infection, may not recover for a long time, such as oral ulcer, Chronic leg ulcer, gastric ulcer, duodenal ulcer etc..A variety of causes leads to
Skin and mucosa ulcer belong to commonly encountered diseases, frequently-occurring disease, conventional Drug therapy can control symptom to a certain extent, but logical
Often due to reasons such as effect is poor, slowly effect, course for the treatment of length, medication troubles, patient is difficult to adhere to；May enter in drug administration process simultaneously
One step aggravation gastrointestinal tract burden, and the toxic and side effects of liver, kidney may be caused.Therefore, what searching was natural has ulcer reparation
The natural Chinese medicines of the medicine-food two-purpose of effect are ideal approach, and many traditional Chinese medicinal seeds oil generally have good guarantor
Strong effect, can not only provide people's diet, and have deviation-correcting function, have " preventive treatment of disease " effect.
Additionally, continuous improvement quality of life being required with people, people increasingly focus on balanced in nutrition it is desirable to pass through
The health product of meals or auxiliary, to adjust the absorption of all kinds of nutrient substance, are kept fit, are built up resistance and prevention disease.
Various health product are a lot of in the market, but have no the report of the compound nutritional mixed oil with ulcer repair.
Content of the invention
Goal of the invention: it is an object of the invention to provide a kind of compound nutritional mixed oil with ulcer repair, its
There is nutrition and Mucous rehabilitation function, the various ulcer including oral ulcer, skin ulcer, gastric ulcer can be had good
Good repair；It is a further object of the present invention to provide the preparation method and application of above-mentioned compound nutritional mixed oil.
Technical scheme: in order to realize object above, the technical scheme that the present invention takes is:
A kind of compound nutritional mixed oil with ulcer repair, it is by Abelmoschus esculentus seed oil, Fructus Cannabis oil and auxiliary oil
Composition.
Preferably, the above-described compound nutritional mixed oil with ulcer repair, described auxiliary oil is
One or more of Oleum Arachidis hypogaeae semen, Oleum sesami, soybean oil, Semen Maydis oil, Oleum Brassicae campestriss, olive oil, Oleum Helianthi, Semen Lini oil.
Preferably, the above-described compound nutritional mixed oil with ulcer repair, described auxiliary oil is
Soybean oil.
Preferably, the above-described compound nutritional mixed oil with ulcer repair, Abelmoschus esculentus seed oil
Total percentage composition with Fructus Cannabis oil is 40～95%, and the percentage composition of auxiliary oil is 5～60%.
As more preferred scheme, the above-described compound nutritional mixed oil with ulcer repair, Abelmoschus esculentus kind
Total percentage composition of seed oil and Fructus Cannabis oil is 70～90%, and the percentage composition of auxiliary oil is 10～30%.
Preferably, the above-described compound nutritional mixed oil with ulcer repair, Abelmoschus esculentus seed oil
With the weight of Fructus Cannabis oil than for 1:9～9:1.
As more preferred scheme, the above-described compound nutritional mixed oil with ulcer repair, Abelmoschus esculentus kind
The weight of seed oil and Fructus Cannabis oil is than for 1:1～4:1.
As particularly preferred scheme, the above-described compound nutritional mixed oil with ulcer repair, Abelmoschus esculentus oil
Total percentage composition is 48%, and the total percentage composition of Fructus Cannabis oil is 32%, and the auxiliary oil total percentage composition of soybean oil is 20%.
The preparation method of the compound nutritional mixed oil with ulcer repair of the present invention, it includes following step
Rapid:
(1) take Abelmoschus esculentus dry seed, pulverize, then adopt cold pressing, cold-maceration, solvent extraction or co2Supercritical fluid
Extraction, obtains Abelmoschus esculentus seed oil, standby；
(2) take band shell Urtica cannabina L. seed, vacuum drying, pulverize, sieve, then adopt cold-press, cold-maceration, solvent extraction or
co2Supercritical fluid extraction, prepares hemp seed oil, standby；
(3) hemp seed oil that the Abelmoschus esculentus seed oil preparing step (1) and step (2) prepare is mixed with auxiliary oil
Uniformly, standing, filtration, fill, packaging.
Preferably, the preparation method of the above-described compound nutritional mixed oil with ulcer repair, institute
The auxiliary oil stated is one of Oleum Arachidis hypogaeae semen, Oleum sesami, soybean oil, Semen Maydis oil, Oleum Brassicae campestriss, olive oil, Oleum Helianthi, Semen Lini oil
Or it is multiple.
Preferably, the preparation method of the above-described compound nutritional mixed oil with ulcer repair, yellow
The co of Flos abelmoschi manihot seed oil2Supercritical fluid extraction condition is: pressure is 25mpa, temperature is 35-40 DEG C, extraction time is 90-
180 minutes, carbon dioxide was 150-200kg/h by the flow of extraction kettle.
Preferably, the preparation method of the above-described compound nutritional mixed oil with ulcer repair, fire
The co of Oleum Sesami2Supercritical fluid extraction condition is: co2Flow velocity 18-23l/h, 20～40 DEG C of extraction temperature, in front 0.5-1.0h
Extracting pressure is 15～20mpa, and then extracting pressure is increased to 20～35mpa, continues extraction 1.0～2.0h.
The compound nutritional mixed oil with ulcer repair of the present invention include oral ulcer, skin ulcer and
Application in interior various ulcer are repaired for the gastric ulcer.
The characteristic that the primary raw material of the present invention has and function are:
Abelmoschus esculentus oil: Abelmoschus esculentus are nutritious, contains substantial amounts of stick-slip juice in young fruit, has special fragrance.Often
Edible have the effect of stomach invigorating intestinal, nourishing negative and positive.Unsaturated fatty acid human consumption's absorbance of Flos abelmoschi manihot oil is very high, can reduce stomach
Acid, stop the function that the disease such as gastritis and duodenal ulcer occurs, being eaten for a long time can effective constipation relieving.
Fructus Cannabis oil: Fructus Cannabiss sweet in the mouth, mild-natured, returns spleen, stomach, large intestine channel, effect loosening bowel to relieve constipation, moisturize the function of parasite killing.
Fructus Cannabiss are one of integration of edible and medicinal herbs kinds of Ministry of Public Health approval, and usage history among the people is long.Compendium of Material Medica is recorded, and Fructus Cannabiss have
There is invigorating the spleen and replenishing QI.
The present invention is analyzed to raw material ingredient each in compound nutritional mixed oil:
Fructus Cannabiss and Abelmoschus esculentus relatively contain abundant Palmic acid, its satisfied fatty acid: monounsaturated fatty acid: how unsaturated
The human consumption fatty acid oil standard (satisfied fatty acid: monounsaturated fatty acid: how not that fatty acid proportion is recommended close to who
The ratio of the amount of saturated fat acid substance is 1:1:1), can be used as edible oil.Additionally, Fructus Cannabiss have containing higher linoleic acid and Asia
Fiber crops acid, and the ratio of linoleic acid plus linolenic acid content is 2.6:1, meets the absorption ratio of the high linolenic of modern nutriology promotion
Example, i.e. linoleic acid: linolenic acid * 100%≤25%；Containing abundant Palmic acid in okra seed oil.
The chemical composition of the various single seed oil of composition mixed oil is as follows:
Beneficial effect: the compound nutritional mixed oil with ulcer repair that the present invention provides, support according to Chinese medicine
Life, health care theory and modern pharmacological research achievement, are obtained by many experiments screening.With Abelmoschus esculentus seed oil and Fructus Cannabis oil it is
Primary raw material is made.Test result indicate that, the compound nutritional mixed oil that the present invention provides can treat oral ulcer, skin is burst
Multiple ulcer such as infectionss and acute and chronic gastric ulcer, can substantially reduce ulcerative hemorrhage area and ulcer score.Burst treating acute stomach
In the application of infectionss, the compound nutritional mixed oil that the present invention provides can reduce ethanol gastric ulcer animal serum proinflammatory factor tnf-
α, il-6, increase the content of anti-inflammatory factors il-10, thus having significant anti-inflammatory activity；Aspirin can be substantially reduced
Gastric ulcer animal serum tumor necrosis factor tnf- alpha content；Gastric ulcer animal gastric tissue no and sod content can be increased, reduce mpo
Content, thus have significant vasodilator and antioxidant activity；Each group compound nutritional mixed oil all can substantially reduce Ah Si
Myeloperoxidase (MPO) mpo content in woods gastric ulcer animal gastric tissue.Additionally, each group compound nutritional mixed oil all can reduce stomach bursting
The content of glutamate pyruvate transaminase (alt), glutamic oxaloacetic transaminase, GOT (ast) and alkali phosphatase (akp) in infectionss animal liverss tissue, thus have
There is liver-protecting activity.
The preparation method that the present invention provides, whole preparation technology design and, high degree of automation, extraction efficiency is high, can be very
Retain well the effective ingredient in raw material, impurity is few, has no toxic side effect.
Specific embodiment
Be further elucidated with the present invention with reference to specific embodiment it should be understood that these embodiments be merely to illustrate the present invention and
It is not used in restriction the scope of the present invention, after having read the present invention, the various equivalence shapes to the present invention for the those skilled in the art
The modification of formula all falls within the application claims limited range.
Embodiment 1
1st, a kind of compound nutritional mixed oil with ulcer repair, it is by the edible line of oils of following percentage by weight
Become: Abelmoschus esculentus oil 4%, Fructus Cannabis oil 36%, soybean oil 60%.Above-mentioned several raw oils are mixed with agitator, stirs under room temperature
Mix 25-35 minute, filter, sealing, 4 DEG C of refrigerator storages.For gastric infusion, the used time fully shakes up.
2nd, there is the preparation method of the compound nutritional mixed oil of ulcer repair, it comprises the following steps:
(1) take Abelmoschus esculentus dry seed, pulverize, then adopt co2Supercritical fluid extraction, obtains Abelmoschus esculentus seed oil,
Standby.co2Supercritical fluid extraction condition is: pressure is 25mpa, temperature is 35-40 DEG C, extraction time is 90-180 minute,
Carbon dioxide is 150-200kg/h by the flow of extraction kettle.
(2) take band shell Urtica cannabina L. seed, vacuum drying, pulverize, sieve, then adopt co2Supercritical fluid extraction, prepares
Hemp seed oil, standby.co2Flow velocity 18-23l/h, 20～40 DEG C of extraction temperature, the extracting pressure in front 0.5-1.0h be 15～
20mpa, then extracting pressure be increased to 20～35mpa, continue extraction 1.0～2.0h.
(3) hemp seed oil that the Abelmoschus esculentus seed oil preparing step (1) and step (2) prepare is mixed with soybean oil
Close uniformly, standing, filtration, fill, packaging.
Embodiment 2
1st, a kind of compound nutritional mixed oil with ulcer repair, it is by the edible line of oils of following percentage by weight
Become:
Abelmoschus esculentus oil 10%, Fructus Cannabis oil 40%, soybean oil 50%.Above-mentioned several raw oils are mixed with agitator, room
Temperature is lower to stir 25-35 minute, filters, sealing, 4 DEG C of refrigerator storages.For gastric infusion, the used time fully shakes up.
2nd, there is the preparation method of the compound nutritional mixed oil of ulcer repair, it comprises the following steps:
(1) take Abelmoschus esculentus dry seed, pulverize, then adopt cold-press, obtain Abelmoschus esculentus seed oil, standby；
(2) take band shell Urtica cannabina L. seed, vacuum drying, pulverize, sieve, then adopt cold-press, prepare hemp seed oil, standby
With；
(3) hemp seed oil that the Abelmoschus esculentus seed oil preparing step (1) and step (2) prepare is mixed with soybean oil
Close uniformly, standing, filtration, fill, packaging.
Embodiment 3
1st, a kind of compound nutritional mixed oil with ulcer repair, it is by the edible line of oils of following percentage by weight
Become:
Abelmoschus esculentus oil 24%, Fructus Cannabis oil 36%, soybean oil 40%.Above-mentioned several raw oils are mixed with agitator, room
Temperature is lower to stir 25-35 minute, filters, sealing, 4 DEG C of refrigerator storages.For gastric infusion, the used time fully shakes up.
2nd, there is the preparation method of the compound nutritional mixed oil of ulcer repair, it comprises the following steps:
(1) take Abelmoschus esculentus dry seed, pulverize, then adopt cold-maceration to extract, obtain Abelmoschus esculentus seed oil, standby；
(2) take band shell Urtica cannabina L. seed, vacuum drying, pulverize, sieve, then adopt cold-maceration to extract, prepare hemp seed oil,
Standby；
(3) hemp seed oil that the Abelmoschus esculentus seed oil preparing step (1) and step (2) prepare is mixed with soybean oil
Close uniformly, standing, filtration, fill, packaging.
Embodiment 4
1st, a kind of compound nutritional mixed oil with ulcer repair, it is by the edible line of oils of following percentage by weight
Become:
Abelmoschus esculentus oil 35%, Fructus Cannabis oil 35%, soybean oil 30%.Above-mentioned several raw oils are mixed with agitator, room
Temperature is lower to stir 25-35 minute, filters, sealing, 4 DEG C of refrigerator storages.For gastric infusion, the used time fully shakes up.
2nd, there is the preparation method of the compound nutritional mixed oil of ulcer repair, it comprises the following steps:
(1) take Abelmoschus esculentus dry seed, pulverize, then adopt solvent extraction to extract, obtain Abelmoschus esculentus seed oil, standby
With；
(2) take band shell Urtica cannabina L. seed, vacuum drying, pulverize, sieve, then adopt solvent extraction to extract, prepare Urtica cannabina L.
Oil, standby；
(3) hemp seed oil that the Abelmoschus esculentus seed oil preparing step (1) and step (2) prepare is mixed with soybean oil
Close uniformly, standing, filtration, fill, packaging.
Embodiment 5
1st, a kind of compound nutritional mixed oil with ulcer repair, it is by the edible line of oils of following percentage by weight
Become:
Abelmoschus esculentus oil 48%, Fructus Cannabis oil 32%, soybean oil 20%.Above-mentioned several raw oils are mixed with agitator, room
Temperature is lower to stir 25-35 minute, filters, sealing, 4 DEG C of refrigerator storages.For gastric infusion, the used time fully shakes up.
The preparation method of Abelmoschus esculentus oil and Fructus Cannabis oil is with embodiment 1.
Embodiment 6
1st, a kind of compound nutritional mixed oil with ulcer repair, it is by the edible line of oils of following percentage by weight
Become:
Abelmoschus esculentus oil 72%, Fructus Cannabis oil 18%, soybean oil 10%.Above-mentioned several raw oil agitators are mixed.
The preparation method of Abelmoschus esculentus oil and Fructus Cannabis oil is with embodiment 1.
Embodiment 7
1st, a kind of compound nutritional mixed oil with ulcer repair, it is by the edible line of oils of following percentage by weight
Become:
Abelmoschus esculentus oil 85.5%, Fructus Cannabis oil 9.5%, soybean oil 5%.Above-mentioned several raw oils are mixed with agitator,
Stir 25-35 minute under room temperature, filter, sealing, 4 DEG C of refrigerator storages.For gastric infusion, the used time fully shakes up.
The preparation method of Abelmoschus esculentus oil and Fructus Cannabis oil is with embodiment 1.
Embodiment 8 oral ulcer effect experiment
By the compound nutritional mixed oil in above-described embodiment 1-7 by weight configuring, it is applied in the direct external in affected part, or with
Certain substrate is mixed and made into membrane, aerosol etc. and is sprayed at ulcer surface, and 4 times a day, during serious symptom sprayable for several times.
For showing the therapeutic effect to oral ulcer for the compound nutritional mixed oil of the present invention, the present invention chooses 50 oral cavities and bursts
Infectionss patient, the compound nutritional mixed oil that the spraying embodiment of the present invention 5 prepares carries out clinical observation, it is found that it can be obvious
Shorten the course of disease, reduce ulcer surface, mitigate patient pain, do not find obvious toxic-side effects.Its curative effect statistical result is as follows: Huan Zhenian
20 years old -50 years old age, male, each 25 people of women, the compound nutritional mixed oil of the application present invention is fully recovered after 3 days 36 people (72%), 5 days
Interior recovery from illness 13 people (26%), fails 1 people (2%) that fully recovers for 5 days.Total effective rate is 98%.
Embodiment 9 skin ulcer effect experiment
By the compound nutritional mixed oil in above-described embodiment 1-7 by weight configuration in case external, or make membrane, cream
Agent etc..Compound nutritional in the present invention is in harmonious proportion oil nature gently, can be used for a variety of causes such as wound, burn, scald, acne and draws
Nutrient after the skin ulcer rising and wound repair, have no toxic side effect.First carried out with hydrogen peroxide or normal saline during application
The cleaning of ulcer surface, takes this compound nutritional mixed oil to apply in wound surface in right amount, and is coated with right amount to wound surface periphery normal skin
On, with sterile gauze and immobilization with adhesive tape, daily or dressing every other day is once.It is found that 5-7 days local pain relief, exudate subtracts
Few, focus is stablized and is reduced, and the oozing of blood position of ulcer surface is dry and hard, and gradually comes off, the no cicatrix of the epidermis after coming off.In the observation period
Interior, the sensation of tested patients' pain and (or) prurituss substantially mitigates, no any exception such as the mental status, appetite, colour of skin.
Embodiment 10 acute gastric ulcer effect experiment
1st, positive control drug
Omeprazole Enteric-coated Capsules: Beijing Yuekang Pharmaceutical Group Co., Ltd..Specification: 20mg/ grain, 14/bottle.Clinic is given
Prescription formula is oral, once a grain, daily 1-2 time.Clinical practice is in gastric ulcer, duodenal ulcer, stress ulcer etc..Produce
Product authentication code: Chinese medicines quasi-word h20056577, shelf-life: 24 months.Compound method: unplug capsule, pour out content, 0.5%
Cmc-na solution prepare, ultrasonic dissolution assisting, before use shaking make mixing.Sealing, 4 DEG C of refrigerator storages.For gastric infusion, used time
Fully shake up, be administered remaining medicinal liquid every time and discard.
Ranitidine hydrochloride capsuless: Shanghai Heng Shan pharmaceutcal corporation, Ltd.Specification: 150mg/ grain, 30/bottle.Clinical administration
Mode is oral, once a grain, daily 1-2 time.Clinical practice is had a stomachache caused by hyperchlorhydria, heartburn sense, acid regurgitation etc..Product
Authentication code: Chinese medicines quasi-word h31020834, shelf-life: 36 months.Compound method: unplug capsule, pour out content, 0.5%
Cmc-na solution is prepared, ultrasonic dissolution assisting, and shaking before use makes mixing.Sealing, 4 DEG C of refrigerator storages.For gastric infusion, the used time fills
Divide and shake up, be administered remaining medicinal liquid every time and discard.
2nd, reagent
In experiment, the used various reagents box including malonaldehyde is Nanjing and builds up Bioengineering Research Institute's product
Product, 4 DEG C of refrigerator storages of reagent, for measuring serum, stomach and liver specimens.
3rd, laboratory animal
Germline: icr mice, spf level；Sex, quantity: male, 170；Body weight, week old scope: starting weight 18.0 ±
1.0g, 6w, body weight 20.0 ± 1.1g, 7w during administration.Source: be purchased from Yangzhou University's comparative medicine center, grade: spf level, experiment
The animal quality certification is numbered: no.201603672, animal productiong licensing number: scxk (Soviet Union) 2012-0004；Feeding environment condition:
Barrier environment system: be applied to spf level Mouse feeder；Humiture: 20～25 DEG C of temperature, relative humidity 40～70%；Ventilation time
Number: 10～20 times/hour；Lighting hours: daily 12h/12h replaces illumination；Clean and sterilize: daily sterilization ground during test
Face, table top and metope, disinfectant is benzalkonium bromide, lysol, ethanol are used alternatingly.
4th, test method
4.1 animal packets and identification
Male icr mice, adapt to raise one week after, random packet, respectively blank group, model group (ethanol model and Ah
Department's 2 models of a woods), each administration group (ethanol model and 2 models of aspirin).Blank control group and model group are respectively sky
White matched group and model group；The compound nutritional mixed oil treatment group that bo1-bo7 prepares for the embodiment of the present invention 1～7；Ao is difficult to understand
The positive drug control group that azoles is ethanol model is drawn by U.S.A；Le ranitidine is the positive drug control group of aspirin model.Labelling side
Method is picric acid staining, in conjunction with group numbering cage tool numbering and individual numbering, all animals is numbered.
4.2 route of administration and dose design
All take gastric infusion mode.Blank control group and model group give same volume distilled water simultaneously, and each administration group is pressed
10ml/kg dosage gastric infusion (bo1-bo7), omeprazole Mouse oral dosage is about 5.7mg/kg, ranitidine mice mouth
Take dosage and be about 42.8mg/kg, once a day, weigh in before gavage.
4.3 operating procedure
Carry out prevention administration according to above-mentioned packet situation and medication, daily gastric infusion once, continuous one week.Ethanol
Cause acute gastric ulcer model: it is positive control drug that this model adopts ranitidine, and each group continuous gavage is administered one week, in experiment knot
Restraint fasting in first 24 hours, other each groups group gavage dehydrated alcohol (10ml/kg) cause acute gastric ulcer model in addition to blank group.Make
After mould 1 hour, pluck eyeball and take blood de- cervical vertebra execution.Blood stands 0.5 hour, and 3000rpm centrifugation 10min (4 DEG C) takes
Clearly, -80 DEG C of Refrigerator stores are standby.Take stomach, small intestinal, liver, kidney, ice brine.Stomach is cut off along greater gastric curvature, removes food
Gruel, normal saline flushing is clean, vernier caliper measurement gastrorrhagia wound surface length and area, and is scored.With formalin pair
Part gastric tissue is fixed, paraffin embedding stripping and slicing, and he dyeing carries out Histopathology assessment.
Aspirin causes acute gastric ulcer model: it is positive control drug that this model adopts omeprazole, each group continuous gavage
Administration one week, terminates fasting in first 24 hours in experiment, other each group group gavage aspirin cmc-na solution in addition to blank group
(20mg/kg) cause acute gastric ulcer model.After modeling 4 hours, pluck eyeball and take blood de- cervical vertebra execution.Blood standing 0.5 is little
When, 3000rpm centrifugation 10min (4 DEG C) takes supernatant, and -80 DEG C of Refrigerator stores are standby.Take stomach, small intestinal, liver, kidney, ice physiology
Salt water washing.Stomach is cut off along greater gastric curvature, removes chyme, and normal saline flushing is clean, vernier caliper measurement gastrorrhagia wound surface length
And area, and scored.With formalin, part gastric tissue is fixed, paraffin embedding stripping and slicing, he dyeing is organized
Pathological evaluation.
Testing index: mice serum tumor necrosis factor tnf- α, interleukin il-6, il-10, TOTAL BILE ACID TBA tba, gallbladder are red
Plain tbil；Nitric oxide no, myeloperoxidase (MPO) mpo, superoxides gasification enzyme sod level are surveyed in gastric tissue homogenate；Hepatic tissue is even
Slurry surveys glutamate pyruvate transaminase alt, glutamic oxaloacetic transaminase, GOT ast, alkali phosphatase akp level.Each index all with kit measurement, illustratively
After absorbance od value surveyed by book, to calculate each index according still further to description.
4.4 data statistics processing methods
Each administration group data is calculated, statistical analysiss is carried out using spss, compares with blank control group and model group respectively
Carry out t inspection, judge significant difference.
5. result of the test
5.1 Animal Behavior Science and Anatomical Observation
Ethanol model group animal gait difficulty, drowsiness, lethargy is depressed, and each treatment group (bo1-bo7) is moved compared with model group
Thing is regained consciousness.Inside coat of the stomach after each group zootomy, mucosa structure assumes the distinguishable greatest differences of naked eyes.It can be seen that model group animal stomach
Inside wall, significantly linear hemorrhagic ulcer in mucosa, and hemorrhagic areas area is big, ulcer score height (table 1)；Additionally, occurring bright
Aobvious submucosa showed edema, mucosal erosion, ulcer, necrosis.Each group compound nutritional mixed oil prophylactic treatment group (bo1-bo7) ulcer
Extent of hemorrhage all obtains different degrees of improvement, especially improves ulcer effect with embodiment 4～6 compound nutritional mixed oil the most aobvious
Write.
Table 1. Alcoholic acute gastric ulcer murine lesion area and ulcer score
Compare with blank control group,*P < 0.05,**P < 0.01,***p<0.001.Compare with model group,#P < 0.05,##p<
0.01,###p<0.001.
5.2 Biochemical Indices In Serum
Ethanol causes acute gastric ulcer model: each group animal serum total bilirubin (tbil) and relevant inflammatory factors (tnf-
α, il-6, il-10) content is shown in Table 2.As known from Table 2, compare with blank control group, Alcoholic gastric ulcer model group mice serum
Inflammatory factor tnf- α, il-6 content substantially increases (p < 0.05, p < 0.01), anti-inflammatory factors il-10 content be remarkably decreased (p <
0.05), total bilirubin tbil content no significant changes.And under same experimental condition, positive drug ranitidine can substantially reduce
Proinflammatory factor tnf- α, il-6, increase the content of anti-inflammatory factors il-10, thus having significant anti-inflammatory activity；Each group is combined battalion
There is different degrees of mitigation in foster mixed oil treatment group to above-mentioned inflammatory factor index, especially with embodiment 5 mediation line of oils effect
Fruit is the most notable, can adjust each inflammatory factor and recover to close to blank control group.In the model, each group compound nutritional is in harmonious proportion
Oily administration group total bilirubin tbil content all has different degrees of decline.
Table 2 Alcoholic gastric ulcer model serum biochemistry data analysiss
Compare with blank control group,*P < 0.05,**p<0.01.Compare with model group,#P < 0.05,##P < 0.01,###p<
0.001.
Aspirin causes acute gastric ulcer model: each group animal serum total bilirubin (tbil) and relevant inflammatory factors
(tnf- α, il-6, il-10) content is shown in Table 3.As known from Table 3, compare with blank control group, aspirin gastric ulcer model group
Mice serum total bilirubin tbil content is declined slightly, but and no difference of science of statistics；Inflammatory factor tnf- α, il-6 content is obvious
Increase (p < 0.01), anti-inflammatory factors il-10 content is remarkably decreased (p < 0.01).And under same experimental condition, each group compound nutritional
There is different degrees of mitigation in mixed oil treatment group to These parameters, and especially embodiment 5 mixed oil equally can significantly reduce
Serum tnf- α, il-6 content of aspirin induced Acute gastric ulcer, dramatically increases containing of serum anti-inflammatory factors il-10 simultaneously
Amount；Positive drug omeprazole can substantially reduce proinflammatory factor tnf- α, il-6, increases the content of anti-inflammatory factors il-10, thus
There is significant anti-inflammatory activity.It should be noted that the anti-inflammatory activity of embodiment 5 mixed oil is almost drawn with positive control drug Aomei
Azoles is suitable.
Table 3 aspirin gastric ulcer model serum biochemistry data analysiss
Compare with blank control group,*P < 0.05,**p<0.01.Compare with model group,#P < 0.05,##P < 0.01,###p<
0.001.
5.3 gastric tissue homogenate biochemical indicators
Take each group animal gastric tissue from -80 DEG C of refrigerators, weigh the gastric tissue of about 100mg, eye scissorss shred, by 5ml/g
Amount add pre-cooling normal saline, carry out tissue homogenate in ice bath.After homogenate, 3000rpm centrifugation 10min (4 DEG C) takes supernatant,
Detected by each experiment box operating instruction, simultaneously albumen in the application each tissue homogenate of bca protein quantification kit measurement
Matter content.
Ethanol causes acute gastric ulcer model: nitric oxide (no) and oxidative stress index marrow in the homogenate of each group animal gastric tissue
Peroxidase (mpo) and superoxides gasification enzyme (sod) content are shown in Table 4.As known from Table 4, compare with blank control group, ethanol
In gastric ulcer model group mice gastric tissue, no and sod content is decreased obviously (p < 0.05), mpo content be remarkably decreased (p <
0.01).And under same experimental condition, positive drug omeprazole can dramatically increase no and sod content, reduces mpo content, thus
There is significant vasodilator and antioxidant activity.Embodiment mediation 5, embodiment mediation 5 can significantly reduce gastric tissue mpo and contain
Amount, and effect is better than positive control drug, shows that mixed oil embodiment 5, embodiment 5 can significantly improve gastric tissue peroxidating and refer to
Mark mpo；Meanwhile, the increase impaired gastric tissue sod content that each group compound nutritional mixed oil treatment group can be different degrees of, improves stomach
Tissue oxidation resistance, especially embodiment mediation 5 can significantly improve gastric tissue sod, and effect and positive control drug are suitable.But adjust
With oily treatment group to no index almost without effect, show that mixed oil relaxes to the gastric tissue no blood vessel of Alcoholic gastric ulcer model mice
Zhang Zuoyong.
The homogenate physicochemical data analysis of table 4 Alcoholic gastric ulcer model gastric tissue
Compare with blank control group,*P < 0.05,**p<0.01.Compare with model group,#P < 0.05,##P < 0.01,###p<
0.001.
Aspirin causes acute gastric ulcer model: in the homogenate of each group animal gastric tissue, nitric oxide (no) and oxidative stress refer to
Mark myeloperoxidase (MPO) (mpo) and superoxides gasification enzyme (sod) content are shown in Table 5.As known from Table 5, compare with blank control group,
In aspirin gastric ulcer model group mice gastric tissue, no and sod content is decreased obviously (p < 0.05), and mpo content is remarkably decreased
(p<0.001).And under same experimental condition, positive drug omeprazole can dramatically increase no and sod content, reduces mpo content,
Thus having significant vasodilator and antioxidant activity.Embodiment mixed oil 1, embodiment mixed oil 2 can dramatically increase stomach group
Knit no content, show that the mixed oil based on Abelmoschus esculentus has significant vasodilative effect.Meanwhile, each group compound nutritional mixed oil
Treatment group can reduce gastric tissue mpo content to some extent, and increases impaired gastric tissue sod content, improve gastric tissue and resist
Oxidation -- the ability that antioxidant system is unbalance, especially reduces lipid peroxidation mpo with embodiment 5 mixed oil and improves tissue antioxygen
The activity changed is the most notable.
The homogenate physicochemical data analysis of table 5 aspirin gastric ulcer model gastric tissue
Compare with blank control group,*P < 0.05,**P < 0.01,***p<0.001.Compare with model group,#P < 0.05,##p<
0.01,###p<0.001.
5.4 liver tissue homogenate's biochemical indicators
Take each group animal liverss from -80 DEG C of refrigerators, weigh the liver of about 100mg, eye scissorss shred, by the amount of 5ml/g
Add the normal saline of pre-cooling, in ice bath, carry out tissue homogenate.After homogenate, 3000rpm centrifugation 10min (4 DEG C) takes supernatant, by each
Individual experiment box operating instruction is detected, in the application each tissue homogenate of bca protein quantification kit measurement, protein contains simultaneously
Amount.
Ethanol causes acute gastric ulcer model: glutamate pyruvate transaminase (alt), glutamic oxaloacetic transaminase, GOT in each group animal liverss tissue
And alkali phosphatase (akp) content is shown in Table 6 (ast).As known from Table 6, compare with blank control group, Alcoholic gastric ulcer model group
In mouse liver tissue, alt, ast and akp substantially increase (p < 0.05, p < 0.01, p < 0.05), and under same experimental condition, sun
Property medicine omeprazole can significantly reduce These parameters, thus having significant liver protecting activities.Mixed oil treatment group can
Improve These parameters to some extent, and embodiment 4～6 mixed oil effect is substantially better than positive control drug, show that mixed oil has
It is significantly improved alcoholic liver damaging action, process of simultaneously taking produces and is nearly free from drug induccd hepatic injury.
The physicochemical data analysis of table 6 Alcoholic gastric ulcer model liver tissue homogenates
Compare with blank control group,*P < 0.05,**p<0.01.Compare with model group,#P < 0.05,##P < 0.01,###p<
0.001.
Aspirin causes acute gastric ulcer model: glutamate pyruvate transaminase (alt), glutamic oxaloacetic transaminase, GOT in each group animal liverss tissue
And alkali phosphatase (akp) content is shown in Table 7 (ast).As known from Table 7, compare with blank control group, aspirin gastric ulcer mould
In type group mouse liver tissue, alt and akp content is slightly increased, and ast dramatically increases.And under same experimental condition, positive drug is difficult to understand
U.S.A draws azoles can reduce These parameters content.Mixed oil treatment group can reduce above-mentioned 3 indexs, is especially adjusted with embodiment 4～6
The most obvious to ast improvement result with oil.
The physicochemical data analysis of table 7 aspirin gastric ulcer model liver tissue homogenates
Compare with blank control group,*P < 0.05,**p<0.01.Compare with model group,#P < 0.05,##P < 0.01,###p<
0.001.
Above test result indicate that, the compound nutritional mixed oil that the present invention provides can protect the gastric acid that many reasons lead to
Acute and chronic gastric ulcer caused by supersecretion, adds prevention by diet and can effectively alleviate multiple gastric ulcers, especially
Alcoholic and aspirin gastric ulcer: bleeding area and the ulcer score of ethanol gastric ulcer can be substantially reduced；Can be notable
Reduce gastric ulcer animal serum proinflammatory factor content, i.e. tumor necrosis factor tnf- α and interleukin il-6, increase anti-inflammatory factors
The content of il-10, thus have significant anti-inflammatory activity；Gastric ulcer animal gastric tissue sod content can be increased simultaneously, reduce
Mpo content, thus have notable antioxidant activity；And can reduce gastric ulcer animal liverss tissue in glutamate pyruvate transaminase (alt),
Glutamic oxaloacetic transaminase, GOT (ast) and the content of alkali phosphatase (akp), thus have liver-protecting activity.It is a kind of health care of green safety
Product, have no adverse reaction.Compared by the mixed oil antiulcer activity of different ratio, the antiulcer of embodiment 5 mixed oil is lived
The strongest, the i.e. Abelmoschus esculentus oil 48% of property, Fructus Cannabis oil 32%, the anti-acute alcoholic of compound mixed oil of soybean oil 20% and A Si
Woods gastric ulcer best results.
The above is only the preferred embodiment of the present invention it is noted that ordinary skill people for the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (11)
1. there is the compound nutritional mixed oil of ulcer repair it is characterised in that it by Abelmoschus esculentus seed oil, Fructus Cannabis oil and
Auxiliary line of oils becomes.
2. the compound nutritional mixed oil with ulcer repair according to claim 1 is it is characterised in that described is auxiliary
Oil is one of Oleum Arachidis hypogaeae semen, Oleum sesami, soybean oil, Semen Maydis oil, Oleum Brassicae campestriss, olive oil, Oleum Helianthi, Semen Lini oil or many
Kind.
3. the compound nutritional mixed oil with ulcer repair according to claim 1 is it is characterised in that described is auxiliary
Oil is soybean oil.
4. the compound nutritional mixed oil with ulcer repair according to any one of claims 1 to 3, its feature exists
In total percentage composition of Abelmoschus esculentus seed oil and Fructus Cannabis oil is 40～95%, and the percentage composition of auxiliary oil is 5～60%.
5. the compound nutritional mixed oil with ulcer repair according to claim 4 is it is characterised in that Abelmoschus esculentus kind
The weight of seed oil and Fructus Cannabis oil is than for 1:9～9:1.
6. the compound nutritional mixed oil with ulcer repair according to claim 1 is it is characterised in that it is by percentage
Content is 48% Abelmoschus esculentus oil, and percentage composition is 32% Fructus Cannabis oil, and percentage composition is 20% soybean oil composition.
7. the preparation method of the compound nutritional mixed oil with ulcer repair described in any one of claim 1～6, it is special
Levy and be, comprise the following steps:
(1) take Abelmoschus esculentus dry seed, pulverize, then adopt cold-press, cold-maceration, solvent extraction or co2Supercritical fluid extracts
Take, obtain Abelmoschus esculentus seed oil, standby；
(2) take band shell Urtica cannabina L. seed, vacuum drying, pulverize, sieve, then adopt cold-press, cold-maceration, solvent extraction or co2Surpass and face
Boundary's fluid extraction, prepares hemp seed oil, standby；
(3) hemp seed oil that the Abelmoschus esculentus seed oil preparing step (1) and step (2) prepare is mixed all with auxiliary oil
Even, standing, filtration, fill, packaging.
8. the preparation method of the compound nutritional mixed oil with ulcer repair described in claim 7 is it is characterised in that institute
The auxiliary oil stated is one of Oleum Arachidis hypogaeae semen, Oleum sesami, soybean oil, Semen Maydis oil, Oleum Brassicae campestriss, olive oil, Oleum Helianthi, Semen Lini oil
Or it is multiple.
9. the preparation method of the compound nutritional mixed oil with preventing and treating acute gastric ulcer effect described in claim 7, its feature
It is, the co of step (1) Abelmoschus esculentus seed oil2Supercritical fluid extraction condition is: extracting pressure be 30mpa, temperature be 45～
50 DEG C, extraction time be 90～180 minutes, carbon dioxide is 200～250kg/h by the flow of extraction kettle；Hemp seed oil
Co2Supercritical fluid extraction condition is: co2Flow velocity 15-25l/h, 25～45 DEG C of extraction temperature, the extraction in front 0.5-1.0h
Pressure is 15～25mpa, and then extracting pressure is increased to 25～35mpa, continues extraction 1.0～2.0h.
10. the compound nutritional mixed oil with ulcer repair described in any one of claim 1 to 6 prevents and treats skin in preparation
With the application in the medicine of mucosa ulcer and health food.
The compound nutritional mixed oil of 11. present invention also can be combined with pharmaceutically acceptable carrier, according to conventional pharmaceutical methods
Make soft capsule, membrane or ointment.
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Cited By (1)
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|---|---|---|---|---|
| CN107736621A (en) * | 2017-09-22 | 2018-02-27 | 华侨大学 | A kind of setose abelmoschus seed fat capsule and preparation method thereof |
Citations (2)
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|---|---|---|---|---|
| CN105341185A (en) * | 2015-11-11 | 2016-02-24 | 成都圣弘源生物科技有限公司 | Composite nutritional blend oil and preparation method thereof |
| CN105660884A (en) * | 2016-01-20 | 2016-06-15 | 恩施州地壹嘉知识产权服务有限公司 | Selenium-rich Abelmoschus esculentus and Camellia oil |
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2016
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105341185A (en) * | 2015-11-11 | 2016-02-24 | 成都圣弘源生物科技有限公司 | Composite nutritional blend oil and preparation method thereof |
| CN105660884A (en) * | 2016-01-20 | 2016-06-15 | 恩施州地壹嘉知识产权服务有限公司 | Selenium-rich Abelmoschus esculentus and Camellia oil |
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| CN107736621A (en) * | 2017-09-22 | 2018-02-27 | 华侨大学 | A kind of setose abelmoschus seed fat capsule and preparation method thereof |
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