CN106359659A - Composite nutrient blend oil with ulcer restoration effect, and preparation method and application thereof - Google Patents

Abstract

The invention discloses a compound nutrient blending oil with ulcer repairing effect and its preparation method and application, which is composed of okra seed oil, hemp seed oil and auxiliary oil. The compound nutrient blending oil with ulcer repairing effect provided by the present invention is prepared through a large number of tests and screenings. It is prepared from natural traditional medicinal and edible vegetable oil with multiple health effects. It has no toxic and side effects, is healthy and safe, and the experimental results show that the compound Nutrition blending oil has good mucosal repairing effect and nutritional value, and can significantly repair various ulcers including oral mucosal ulcers, skin surface ulcers, and gastric ulcers. The preparation method provided by the invention has the advantages of design and design of the whole preparation process, high degree of automation, high extraction efficiency, good retention of active ingredients in raw materials, less impurities, and no toxic and side effects.

Description

A compound nutrient blending oil with ulcer repairing effect and its preparation method and application

technical field

The invention relates to a natural vegetable oil composition, in particular to a compound nutrition blending oil with ulcer repairing effect and a preparation method thereof.

Background technique

As the first line of defense of the human immune system, the skin and mucous membranes are an important barrier for the body to defend against the invasion of foreign substances. When the skin and mucous membranes are ulcerated due to trauma, microbial infection, tumor, circulatory disorder, nerve dysfunction, immune function abnormality or congenital skin defects, etc., localized defect and ulceration of the skin or mucous membrane surface tissue, often accompanied by chronic infection, may Prolonged healing, such as oral ulcers, chronic calf ulcers, gastric ulcers, duodenal ulcers, etc. Skin and mucous membrane ulcers caused by various reasons are common and frequently-occurring diseases. Conventional drug treatment can control the symptoms to a certain extent, but usually due to poor effect, slow effect, long course of treatment, and troublesome medication, it is difficult for patients to persist; at the same time In the process of taking the medicine, it may further aggravate the burden on the gastrointestinal tract, and may cause toxic side effects on the liver and kidneys. Therefore, it is a relatively ideal way to find natural pure natural traditional Chinese medicine with ulcer repairing effect. Many traditional Chinese medicine seed oils usually have good health care effects, not only can provide people's daily diet, but also have the function of correcting deviation. prevent disease".

In addition, with the continuous improvement of people's quality of life requirements, people pay more and more attention to nutritional balance, hoping to adjust the intake of various nutrients through diet or supplementary health products, maintain health, enhance resistance and prevent diseases. Various health care products are a lot on the market at present, but do not see the report of the compound nutrient blending oil that has ulcer repairing effect.

Contents of the invention

Purpose of the invention: The purpose of the present invention is to provide a compound nutritional blending oil with ulcer repairing effect, which has nutrition and mucosal repair functions, and can have good repair of various ulcers including oral ulcers, skin ulcers and gastric ulcers Effect; Another object of the present invention is to provide the preparation method and application of the above-mentioned compound nutrition blending oil.

Technical scheme: in order to realize above object, the technical scheme that the present invention takes is:

A compound nutritional blend oil with ulcer repairing effect, which is composed of okra seed oil, hemp seed oil and auxiliary oil.

As a preferred version, the above-mentioned complex nutrition blending oil with ulcer repairing effect, the auxiliary oil is peanut oil, sesame oil, soybean oil, corn oil, rapeseed oil, olive oil, sunflower oil, linseed oil one or more.

As a preferred version, the above-mentioned complex nutrition blending oil with ulcer repairing effect, the auxiliary oil is soybean oil.

As a preferred option, the above-mentioned compound nutrition blending oil with ulcer repairing effect, the total percentage of okra seed oil and hemp seed oil is 40-95%, and the percentage of auxiliary oil is 5-60%.

As a more preferred solution, the above-mentioned compound nutrition blending oil with ulcer repairing effect, the total percentage of okra seed oil and hemp seed oil is 70-90%, and the percentage of auxiliary oil is 10-30%.

As a preferred solution, the weight ratio of the above-mentioned complex nutrient blending oil with ulcer repairing effect, okra seed oil and hemp seed oil is 1:9-9:1.

As a more preferred solution, the weight ratio of the above-mentioned complex nutrient blending oil with ulcer repairing effect, okra seed oil and hemp seed oil is 1:1-4:1.

As a particularly preferred scheme, the above-mentioned compound nutrition blending oil with ulcer repairing effect, the total percentage content of okra oil is 48%, the total percentage content of hemp seed oil is 32%, and the total percentage content of auxiliary oil soybean oil is 20%.

The preparation method of the compound nutrition blending oil with ulcer repairing effect of the present invention, it comprises the following steps:

(1) Get okra dried seeds, pulverize, then adopt cold pressing, cold soaking method, solvent extraction or _CO Supercritical fluid extraction, obtain okra seed oil, for subsequent use;

(2) Get shelled hemp seeds, vacuum dry, pulverize, sieve, then adopt cold pressing method, cold soaking method, solvent extraction or _CO Supercritical fluid extraction, prepare hemp oil, standby;

(3) Mix the okra seed oil prepared in step (1) and the hemp oil and auxiliary oil prepared in step (2) evenly, let stand, filter, fill and pack.

As a preferred version, the preparation method of the above-mentioned compound nutrition blending oil with ulcer repairing effect, the auxiliary oil is peanut oil, sesame oil, soybean oil, corn oil, rapeseed oil, olive oil, sunflower oil, linseed oil One or more of the oils.

As a preferred version, the preparation method of the above-mentioned compound nutrient blending oil with ulcer repairing effect, the _CO supercritical fluid extraction conditions of okra seed oil are: pressure is 25MPa, temperature is 35-40 ° C, extraction time is 90- In 180 minutes, the flow rate of carbon dioxide gas through the extraction tank is 150-200kg/h.

As a preferred option, in the preparation method of the above-mentioned compound nutrient blending oil with ulcer repairing effect, the _CO2 supercritical fluid extraction conditions of hemp oil are: _CO2 flow rate 18-23L/h, extraction temperature 20-40 ° C, before The extraction pressure within 0.5-1.0h is 15-20MPa, then the extraction pressure is increased to 20-35MPa, and the extraction is continued for 1.0-2.0h.

The application of the complex nutrient blending oil with ulcer repairing effect of the present invention in repairing various ulcers including oral ulcers, skin ulcers and gastric ulcers.

The characteristics and functions that the main raw material of the present invention has are:

Okra oil: Okra is rich in nutrition, and the young fruit contains a lot of sticky juice, which has a special fragrance. Regular consumption has the effect of strengthening the stomach and intestines and nourishing yin and yang. The unsaturated fatty acid of okra oil has a high rate of digestion and absorption by the human body, which can reduce gastric acid, prevent gastritis and duodenal ulcer and other diseases, and long-term consumption can effectively relieve constipation.

Hemp Seed Oil: Hemp Seed is sweet in taste and flat in nature, and belongs to the spleen, stomach, and large intestine meridian. Hemp seed is one of the homologous varieties of medicine and food approved by the Ministry of Health, and has a long history of folk use. "Compendium of Materia Medica" records that hemp seed has the effect of invigorating the middle and replenishing qi.

The present invention analyzes the ingredients contained in each raw material in the compound nutrition blending oil:

Hemp seeds and okra are rich in palmitic acid, and the ratio of saturated fatty acid: monounsaturated fatty acid: polyunsaturated fatty acid is close to the fatty acid standard of human edible oil recommended by WHO (saturated fatty acid: monounsaturated fatty acid: polyunsaturated fatty acid substance The amount ratio is 1:1:1), which can be used as edible oil. In addition, hemp seeds contain high linoleic acid and linolenic acid, and the ratio of linoleic acid to linolenic acid content is 2.6:1, which is in line with the high intake ratio of linolenic acid advocated by modern nutrition, that is, linoleic acid : Linolenic acid*100%≤25%; Okra seed oil is rich in palmitic acid.

The chemical composition of various single seed oils that make up the blend oil is as follows:

Beneficial effects: the compound nutritional blending oil with ulcer repairing effect provided by the present invention is obtained through a large number of experimental screenings based on traditional Chinese medicine health preservation, health care theory and modern pharmacological research results. Made with okra seed oil and hemp seed oil as main raw materials. Experimental results show that the compound nutrition blending oil provided by the invention can treat multiple ulcers such as oral ulcers, skin ulcers, acute and chronic gastric ulcers, etc., and can significantly reduce ulcer bleeding area and ulcer score. In the application of treating acute gastric ulcer, the compound nutrition blending oil provided by the present invention can reduce the serum pro-inflammatory factors TNF-α and IL-6 of animals with alcoholic gastric ulcer, and increase the content of anti-inflammatory factor IL-10, thereby having significant Anti-inflammatory activity; can significantly reduce the content of serum tumor necrosis factor TNF-α in animals with aspirin-induced gastric ulcer; can increase the content of NO and SOD in gastric tissue of animals with gastric ulcer, and reduce the content of MPO, so it has significant vasodilation and antioxidant activity; each group Compound nutrition blending oil can significantly reduce the content of myeloperoxidase MPO in the gastric tissue of animals with aspirin-induced gastric ulcer. In addition, each group of compound nutrient blending oils can reduce the contents of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (AKP) in the liver tissue of animals with gastric ulcer, thus having hepatoprotective activity.

The preparation method provided by the invention has the advantages of design and design of the whole preparation process, high degree of automation, high extraction efficiency, good retention of active ingredients in raw materials, less impurities, and no toxic and side effects.

detailed description

The present invention is further illustrated below in conjunction with specific embodiments, should be understood that these embodiments are only used to illustrate the present invention and are not intended to limit the scope of the present invention, after having read the present invention, those skilled in the art will understand the various equivalent forms of the present invention All modifications fall within the scope defined by the appended claims of this application.

Example 1

1, a kind of compound nutrition blending oil with ulcer repairing effect, it is made up of the edible oil of following percentage by weight: 4% of okra oil, 36% of hemp seed oil, 60% of soybean oil. Mix the above several raw material oils with a mixer, stir at room temperature for 25-35 minutes, filter, seal, and store in a refrigerator at 4°C. For oral administration, shake well when used.

2. The preparation method of the compound nutrient blending oil with ulcer repairing effect, which comprises the following steps:

(1) Take dried okra seeds, pulverize them, and then use _CO2 supercritical fluid extraction to obtain okra seed oil for future use. CO ₂ supercritical fluid extraction conditions are: pressure 25MPa, temperature 35-40°C, extraction time 90-180 minutes, flow of carbon dioxide gas through the extraction tank 150-200kg/h.

(2) Take the shelled hemp seeds, vacuum dry, pulverize, sieve, and then use _CO Supercritical fluid extraction to prepare hemp oil for future use. The flow rate of CO ₂ is 18-23L/h, the extraction temperature is 20-40°C, the extraction pressure is 15-20MPa in the first 0.5-1.0h, and then the extraction pressure is increased to 20-35MPa, and the extraction is continued for 1.0-2.0h.

(3) The okra seed oil prepared in the step (1) and the hemp oil and the soybean oil prepared in the step (2) are uniformly mixed, left standing, filtered, filled and packaged.

Example 2

1, a kind of compound nutrition blending oil with ulcer repairing effect, it is made up of the edible oil of following percentage by weight:

Okra oil 10%, hemp seed oil 40%, soybean oil 50%. Mix the above several raw material oils with a mixer, stir at room temperature for 25-35 minutes, filter, seal, and store in a refrigerator at 4°C. For oral administration, shake well when used.

2. The preparation method of the compound nutrient blending oil with ulcer repairing effect, which comprises the following steps:

(1) Get the dried okra seeds, pulverize, then adopt the cold pressing method to obtain the okra seed oil, and set aside;

(2) Get shelled hemp seeds, vacuum-dry, pulverize, sieve, adopt cold pressing method then, prepare hemp oil, set aside;

(3) The okra seed oil prepared in the step (1) and the hemp oil and the soybean oil prepared in the step (2) are uniformly mixed, left standing, filtered, filled and packaged.

Example 3

1, a kind of compound nutrition blending oil with ulcer repairing effect, it is made up of the edible oil of following percentage by weight:

Okra oil 24%, hemp seed oil 36%, soybean oil 40%. Mix the above several raw material oils with a mixer, stir at room temperature for 25-35 minutes, filter, seal, and store in a refrigerator at 4°C. For oral administration, shake well when used.

2. The preparation method of the compound nutrient blending oil with ulcer repairing effect, which comprises the following steps:

(1) Get dried okra seeds, pulverize, then extract by cold soaking to obtain okra seed oil, set aside;

(2) Get shelled hemp seeds, vacuum dry, pulverize, sieve, adopt cold soaking method to extract then, prepare hemp oil, set aside;

(3) The okra seed oil prepared in the step (1) and the hemp oil and the soybean oil prepared in the step (2) are uniformly mixed, left standing, filtered, filled and packaged.

Example 4

1, a kind of compound nutrition blending oil with ulcer repairing effect, it is made up of the edible oil of following percentage by weight:

Okra oil 35%, hemp seed oil 35%, soybean oil 30%. Mix the above several raw material oils with a mixer, stir at room temperature for 25-35 minutes, filter, seal, and store in a refrigerator at 4°C. For oral administration, shake well when used.

2. The preparation method of the compound nutrient blending oil with ulcer repairing effect, which comprises the following steps:

(1) Get dried okra seeds, pulverize, then extract by solvent extraction to obtain okra seed oil, set aside;

(2) Get shelled hemp seeds, vacuum dry, pulverize, sieve, then use solvent extraction to prepare hemp oil, set aside;

(3) The okra seed oil prepared in the step (1) and the hemp oil and the soybean oil prepared in the step (2) are uniformly mixed, left standing, filtered, filled and packaged.

Example 5

1, a kind of compound nutrition blending oil with ulcer repairing effect, it is made up of the edible oil of following percentage by weight:

Okra oil 48%, hemp seed oil 32%, soybean oil 20%. Mix the above several raw material oils with a mixer, stir at room temperature for 25-35 minutes, filter, seal, and store in a refrigerator at 4°C. For oral administration, shake well when used.

The preparation method of okra oil and hemp seed oil is with embodiment 1.

Example 6

1, a kind of compound nutrition blending oil with ulcer repairing effect, it is made up of the edible oil of following percentage by weight:

Okra oil 72%, hemp seed oil 18%, soybean oil 10%. Mix the above-mentioned raw material oils with a mixer.

The preparation method of okra oil and hemp seed oil is with embodiment 1.

Example 7

1, a kind of compound nutrition blending oil with ulcer repairing effect, it is made up of the edible oil of following percentage by weight:

Okra oil 85.5%, hemp seed oil 9.5%, soybean oil 5%. Mix the above several raw material oils with a mixer, stir at room temperature for 25-35 minutes, filter, seal, and store in a refrigerator at 4°C. For oral administration, shake well when used.

The preparation method of okra oil and hemp seed oil is with embodiment 1.

Example 8 Drug efficacy experiment for oral ulcer

The compound nutrient blending oil in the above-mentioned examples 1-7 is configured according to the weight ratio, applied to the affected area for external use directly, or mixed with a certain matrix to make a film, aerosol, etc., sprayed on the ulcer surface, 4 times a day, the symptoms In severe cases, it can be sprayed several times.

In order to show the therapeutic effect of the compound nutrient blending oil of the present invention on oral ulcers, the present invention selects 50 patients with oral ulcers, sprays the compound nutrient blending oil prepared in Example 5 of the present invention for clinical observation, and finds that it can obviously shorten the course of the disease, shrink Ulcer surface, relieve pain of patients, no obvious side effects were found. Its curative effect statistic result is as follows: patient's age is 20 years old-50 years old, each 25 people of male and female, and 36 people (72%) fully recovered after using compound nutrition blending oil of the present invention 3 days, 13 people (26%) fully recovered in 5 days, 5 Days failed to recover 1 person (2%). The total effective rate is 98%.

Example 9 Drug efficacy experiment on skin ulcer

The compound nutrient blending oil in the above-mentioned embodiments 1-7 is configured by weight ratio for external use, or made into film, ointment and the like. The compound nutrient blending oil of the present invention is mild in nature, can be used for nutrition supply and wound surface repair after skin ulcers caused by various reasons such as trauma, burns, scalds, acne, etc., without toxic and side effects. When applying, first clean the ulcer surface with hydrogen peroxide or normal saline, apply an appropriate amount of the compound nutrient blending oil on the wound surface, and spread an appropriate amount on the normal skin around the wound surface, and fix it with sterile gauze and adhesive tape, every day or the next day Change the dressing once. It was found that the local pain was relieved in 5-7 days, the exudate was reduced, the lesion was stable and shrunk, the bleeding part of the ulcer surface was dry and hardened, and gradually fell off, and the peeled epidermis had no scars. During the observation period, the pain and (or) pruritus of the test patients were significantly relieved, and there was no abnormality in mental state, appetite, skin color, etc.

Example 10 Drug efficacy experiment for acute gastric ulcer

1. Positive control drug

Omeprazole enteric-coated capsules: Beijing Yuekang Pharmaceutical Group Co., Ltd. Specifications: 20mg/capsule, 14 capsules/bottle. The clinical administration method is oral administration, one capsule at a time, 1-2 times a day. It is clinically used in gastric ulcer, duodenal ulcer, stress ulcer, etc. Product approval number: Guoyao Zhunzi H20056577, shelf life: 24 months. Preparation method: pull out the capsule, pour out the contents, prepare with 0.5% CMC-Na solution, ultrasonically aid in dissolution, and shake to mix evenly before use. Seal and store in refrigerator at 4°C. For intragastric administration, shake well when using, and discard the remaining medicinal solution after each administration.

Ranitidine Hydrochloride Capsules: Shanghai Hengshan Pharmaceutical Co., Ltd. Specification: 150mg/capsule, 30 capsules/bottle. The clinical administration method is oral administration, one capsule at a time, 1-2 times a day. It is clinically used in gastric pain, heartburn, acid reflux, etc. caused by hyperacidity. Product approval number: Guoyao Zhunzi H31020834, shelf life: 36 months. Preparation method: pull out the capsule, pour out the contents, prepare with 0.5% CMC-Na solution, ultrasonically aid in dissolution, and shake to mix evenly before use. Seal and store in refrigerator at 4°C. For intragastric administration, shake well when using, and discard the remaining medicinal solution after each administration.

2. Reagents

Various kits including malondialdehyde used in the experiment are products of Nanjing Jiancheng Institute of Bioengineering, and the reagents are stored in a refrigerator at 4°C for the determination of serum, stomach and liver samples.

3. Experimental animals

Breed: ICR mice, SPF grade; sex, number: male, 170; body weight, week age range: initial weight 18.0±1.0g, 6w, body weight at the time of administration 20.0±1.1g, 7w. Source: Purchased from the Comparative Medicine Center of Yangzhou University, grade: SPF, experimental animal certificate number: NO.201603672, animal production license number: SCXK (Su) 2012-0004; feeding environment conditions: barrier environment system: applied to SPF Breeding of high-grade mice; temperature and humidity: temperature 20-25°C, relative humidity 40-70%; ventilation frequency: 10-20 times/hour; lighting time: 12h/12h alternate lighting per day; cleaning and disinfection: every day during the test Daily disinfection of the ground, countertops and walls, the disinfectant is Xinjiede, Lysol, and ethanol are used alternately.

4. Test method

4.1 Animal grouping and identification

Male icr mice were randomly divided into blank group, model group (alcohol model and 2 models of aspirin) and each administration group (alcohol model and 2 models of aspirin) after being fed for one week. The blank control group and the model group are the blank control group and the model group respectively; BO1-BO7 are the compound nutritional blending oil treatment groups prepared in Examples 1 to 7 of the present invention; AO omeprazole is the positive drug control group of the ethanol model; LE Ranitidine is the positive drug control group of the aspirin model. The marking method is picric acid staining method, combined with group number, cage number and individual number, to number all animals.

4.2 Administration route and dosage design

All were administered by intragastric administration. The blank control group and the model group were given the same volume of distilled water at the same time, and each administration group was intragastrically administered (BO1-BO7) at a dose of 10ml/Kg. The oral dose of omeprazole in mice was about 5.7mg/kg, and ranitidine The oral dose of mice is about 42.8mg/kg, once a day, and the body weight is weighed before gavage.

4.3 Operation steps

Preventive administration was carried out according to the above-mentioned grouping situation and administration method, and intragastric administration was administered once a day for one week continuously. Ethanol-induced acute gastric ulcer model: this model uses ranitidine as the positive control drug, and each group is administered intragastrically for one week, fasting 24 hours before the end of the experiment, and all groups except the blank group are intragastrically administered absolute ethanol (10ml/Kg) to create an acute gastric ulcer model. After 1 hour of modeling, the eyeballs were removed for blood collection and sacrificed by dislocation of the cervical vertebrae. The blood was allowed to stand still for 0.5 hour, centrifuged at 3000 rpm for 10 min (4°C) to take the supernatant, and stored in a -80°C refrigerator for later use. The stomach, small intestine, liver, and kidney were taken and washed with iced saline. The stomach was cut open along the greater curvature of the stomach to remove chyme, rinsed with normal saline, and the length and area of gastric bleeding wounds were measured with a vernier caliper and scored. Parts of gastric tissue were fixed with formaldehyde solution, embedded in paraffin and cut into sections, and HE stained for histopathological evaluation.

Aspirin-induced acute gastric ulcer model: this model uses omeprazole as the positive control drug, and each group is administered intragastrically for one week, and fasts 24 hours before the end of the experiment. Na solution (20mg/Kg) caused acute gastric ulcer model. After 4 hours of modeling, the eyeballs were removed for blood collection, and the animals were killed by dislocation of the cervical vertebrae. The blood was allowed to stand still for 0.5 hours, centrifuged at 3000 rpm for 10 min (4°C) to take the supernatant, and stored in a -80°C refrigerator for later use. The stomach, small intestine, liver, and kidney were taken and washed with iced saline. The stomach was cut open along the greater curvature of the stomach to remove chyme, rinsed with normal saline, and the length and area of gastric bleeding wounds were measured with a vernier caliper and scored. Parts of gastric tissue were fixed with formaldehyde solution, embedded in paraffin and cut into sections, and HE stained for histopathological evaluation.

Measuring indicators: mouse serum tumor necrosis factor TNF-α, interleukin IL-6, IL-10, total bile acid TBA, bilirubin TBil; stomach tissue homogenate to measure nitric oxide NO, myeloperoxidase MPO, super Oxide gasification enzyme SOD level; liver tissue homogenate to measure the levels of alanine aminotransferase ALT, aspartate aminotransferase AST and alkaline phosphatase AKP. All indicators are determined with kits, after measuring the absorbance OD value according to the instructions, and then calculate the indicators according to the instructions.

4.4 Data statistical processing method

The data of each drug administration group was calculated, and SPSS was used for statistical analysis, and compared with the blank control group and the model group, the t test was carried out to determine the significant difference.

5. Test results

5.1 Animal behavior and anatomical observation

The animals in the alcohol model group had unsteady gait, lethargy, and listlessness, and the animals in each treatment group (BO1-BO7) were more awake than the animals in the model group. After anatomy, the mucosal structure of the inner gastric wall in each group showed a huge difference that could be discerned by naked eyes. It can be seen that obvious linear hemorrhagic ulcers appeared in the inner gastric wall mucosa of the animals in the model group, the bleeding area was large, and the ulcer score was high (Table 1); in addition, there were obvious submucosal edema, mucosal erosion, ulcers, and necrosis. The degree of ulcer bleeding in each group of compound nutrient blending oil preventive treatment groups (BO1-BO7) was improved to varying degrees, especially the effect of compound nutrient blending oil in Examples 4-6 on improving ulcers was the most significant.

Table 1. Ulcer area and ulcer score in mice with alcoholic acute gastric ulcer

Compared with blank control group, ^* p<0.05, ^** p<0.01, ^*** p<0.001. Compared with the model group, ^# p<0.05, ^## p<0.01, ^### p<0.001.

5.2 Serum biochemical indicators

Alcohol-induced acute gastric ulcer model: the contents of serum total bilirubin (Tbil) and related inflammatory factors (TNF-α, IL-6, IL-10) of animals in each group are shown in Table 2. It can be seen from Table 2 that compared with the blank control group, the content of serum inflammatory factors TNF-α and IL-6 in the alcoholic gastric ulcer model group mice increased significantly (p<0.05, p<0.01), and the content of anti-inflammatory factor IL-10 significantly increased. decreased (p<0.05), but there was no significant change in the total bilirubin Tbil content. And under the same test conditions, the positive drug ranitidine can significantly reduce the pro-inflammatory factors TNF-α, IL-6, increase the content of anti-inflammatory factor IL-10, so it has significant anti-inflammatory activity; The treatment groups had different degrees of relieving effects on the above-mentioned inflammatory factor indicators, especially the blended oil group of Example 5 had the most significant effect, and could regulate each inflammatory factor to restore to be close to that of the blank control group. In this model, the total bilirubin Tbil content of the compound nutrition blending oil administration group in each group decreased to varying degrees.

Table 2 Analysis of serum biochemical data of alcoholic gastric ulcer model

Compared with blank control group, ^* p<0.05, ^** p<0.01. Compared with the model group, ^# p<0.05, ^## p<0.01, ^### p<0.001.

Aspirin-induced acute gastric ulcer model: the contents of serum total bilirubin (Tbil) and related inflammatory factors (TNF-α, IL-6, IL-10) of animals in each group are shown in Table 3. It can be seen from Table 3 that compared with the blank control group, the serum total bilirubin Tbil content of mice in the aspirin gastric ulcer model group decreased slightly, but there was no statistical difference; the content of inflammatory factors TNF-α and IL-6 increased significantly ( p<0.01), the content of anti-inflammatory factor IL-10 decreased significantly (p<0.01). And under the same test conditions, each group of compound nutritional blending oil treatment groups has different degrees of relief on the above indicators, especially the blending oil of Example 5 can also significantly reduce the serum TNF-α and IL-6 levels of acute gastric ulcer caused by aspirin , while significantly increasing the content of serum anti-inflammatory factor IL-10; the positive drug omeprazole can significantly reduce pro-inflammatory factors TNF-α, IL-6, increase the content of anti-inflammatory factor IL-10, thus has significant anti-inflammatory active. It is worth noting that the anti-inflammatory activity of the blended oil in Example 5 is almost equivalent to that of the positive control drug omeprazole.

Table 3 Analysis of serum biochemical data of aspirin-induced gastric ulcer model

Compared with blank control group, ^* p<0.05, ^** p<0.01. Compared with the model group, ^# p<0.05, ^## p<0.01, ^### p<0.001.

5.3 Biochemical indicators of gastric tissue homogenate

Stomach tissues of each group of animals were taken from the -80°C refrigerator, and about 100 mg of gastric tissues were weighed, cut into pieces with ophthalmological scissors, and pre-cooled physiological saline was added in an amount of 5 ml/g, and the tissues were homogenized in an ice bath. After homogenization, centrifuge at 3000rpm for 10min (4°C) to take the supernatant, detect according to the operation instructions of each experiment box, and at the same time use the BCA protein quantification kit to measure the protein content in each tissue homogenate.

Alcohol-induced acute gastric ulcer model: the contents of nitric oxide (NO) and oxidative stress indicators myeloperoxidase (MPO) and superoxide gasase (SOD) in the gastric tissue homogenates of animals in each group are shown in Table 4. It can be seen from Table 4 that compared with the blank control group, the contents of NO and SOD in the gastric tissue of mice in the alcoholic gastric ulcer model group decreased significantly (p<0.05), and the content of MPO decreased significantly (p<0.01). Under the same test conditions, the positive drug omeprazole can significantly increase the content of NO and SOD, and reduce the content of MPO, so it has significant vasodilation and antioxidant activity. Embodiment reconciliation 5 and embodiment reconciliation 5 can significantly reduce the MPO content of gastric tissue, and the effect is better than that of the positive control drug, showing that the reconciliation oil embodiment 5 and embodiment 5 can significantly improve the gastric tissue peroxidation index MPO; at the same time, each group compound The nutritional blending oil treatment group can increase the SOD content of the damaged gastric tissue to varying degrees and improve the antioxidant capacity of the gastric tissue. In particular, the blending oil of Example 5 can significantly increase the SOD of the gastric tissue, and the effect is equivalent to that of the positive control drug. However, the blended oil treatment group had almost no effect on the NO index, indicating that the blended oil had no vasodilation effect on the gastric tissue of alcoholic gastric ulcer model mice.

Table 4 Biochemical data analysis of gastric tissue homogenate in alcoholic gastric ulcer model

Compared with blank control group, ^* p<0.05, ^** p<0.01. Compared with the model group, ^# p<0.05, ^## p<0.01, ^### p<0.001.

Aspirin-induced acute gastric ulcer model: the contents of nitric oxide (NO) and oxidative stress indicators myeloperoxidase (MPO) and superoxide gasase (SOD) in gastric tissue homogenates of animals in each group are shown in Table 5. It can be seen from Table 5 that, compared with the blank control group, the contents of NO and SOD in the gastric tissue of mice in the aspirin-induced gastric ulcer model group decreased significantly (p<0.05), and the content of MPO decreased significantly (p<0.001). Under the same test conditions, the positive drug omeprazole can significantly increase the content of NO and SOD, and reduce the content of MPO, so it has significant vasodilation and antioxidant activity. Blended oil 1 and blended oil 2 of Example can significantly increase the NO content of gastric tissue, indicating that the blended oil mainly composed of okra has a significant vasodilator effect. At the same time, each group of compound nutritional blending oil treatment groups can reduce the MPO content of gastric tissue to varying degrees, increase the SOD content of damaged gastric tissue, and improve the ability of gastric tissue to resist oxidation-the imbalance of the anti-oxidation system, especially the blended oil of Example 5 The activity of reducing lipid peroxidation MPO and improving tissue antioxidant activity is the most significant.

Table 5 Analysis of biochemical data of gastric tissue homogenate in aspirin-induced gastric ulcer model

Compared with blank control group, ^* p<0.05, ^** p<0.01, ^*** p<0.001. Compared with the model group, ^# p<0.05, ^## p<0.01, ^### p<0.001.

5.4 Biochemical indicators of liver tissue homogenate

Take the livers of animals in each group from the -80°C refrigerator, weigh about 100 mg of the livers, cut them into pieces with ophthalmic scissors, add pre-cooled saline at an amount of 5 ml/g, and perform tissue homogenization in an ice bath. After homogenization, centrifuge at 3000rpm for 10min (4°C) to take the supernatant, detect according to the operation instructions of each experiment box, and at the same time use the BCA protein quantification kit to measure the protein content in each tissue homogenate.

Alcohol-induced acute gastric ulcer model: the contents of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (AKP) in the liver tissues of animals in each group are shown in Table 6. It can be seen from Table 6 that compared with the blank control group, ALT, AST and AKP in the liver tissue of the mice in the alcoholic gastric ulcer model group were significantly increased (p<0.05, p<0.01, p<0.05), while under the same test conditions, the positive The drug omeprazole can significantly reduce the above indicators, thus having significant liver protection activity. The blended oil treatment group can improve the above indicators to varying degrees, and the effect of the blended oil in Examples 4 to 6 is significantly better than that of the positive control drug, indicating that the blended oil has a significant effect on improving alcohol-induced liver damage, and at the same time, almost no drug source is produced during the administration process. permanent liver damage.

Table 6 Analysis of biochemical data of liver tissue homogenate in alcoholic gastric ulcer model

Compared with blank control group, ^* p<0.05, ^** p<0.01. Compared with the model group, ^# p<0.05, ^## p<0.01, ^### p<0.001.

Aspirin-induced acute gastric ulcer model: the contents of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (AKP) in the liver tissues of animals in each group are shown in Table 7. It can be seen from Table 7 that compared with the blank control group, the contents of ALT and AKP in the liver tissue of mice in the aspirin-induced gastric ulcer model group increased slightly, and AST increased significantly. Under the same test conditions, the positive drug omeprazole can reduce the content of the above indicators. The blended oil treatment group can reduce the above three indicators, especially the blended oils of Examples 4-6 have the most obvious effect on improving AST.

Table 7 Analysis of biochemical data of aspirin-induced gastric ulcer model liver tissue homogenate

Compared with blank control group, ^* p<0.05, ^** p<0.01. Compared with the model group, ^# p<0.05, ^## p<0.01, ^### p<0.001.

The above experimental results show that the compound nutrient blending oil provided by the present invention can protect acute and chronic gastric ulcers caused by excessive gastric acid secretion caused by various reasons, and can effectively relieve various gastric ulcers through daily dietary supplementation prevention, especially alcohol-induced gastric ulcers. And aspirin-induced gastric ulcer: can significantly reduce the bleeding area and ulcer score of alcohol-induced gastric ulcer; can significantly reduce the content of pro-inflammatory factors in the serum of animals with gastric ulcer, that is, tumor necrosis factor TNF-α and interleukin IL-6, and increase the anti-inflammatory factor IL The content of -10, thus has significant anti-inflammatory activity; Can increase gastric ulcer animal gastric tissue SOD content simultaneously, reduce MPO content, thereby have significant antioxidant activity; And can reduce alanine aminotransferase (ALT), in gastric ulcer animal liver tissue, Aspartate aminotransferase (AST) and alkaline phosphatase (AKP) content, so it has hepatoprotective activity. It is a green and safe health care product without adverse reactions. By comparing the anti-ulcer activity of blended oils of different proportions, it can be seen that the anti-ulcer activity of the blended oil in Example 5 is the strongest, that is, the compound blended oil of 48% okra oil, 32% hemp seed oil, and 20% soybean oil has anti-acute alcohol resistance. And aspirin gastric ulcer effect is the best.

The above is only a preferred embodiment of the present invention, it should be pointed out that, for those of ordinary skill in the art, without departing from the principle of the present invention, some improvements and modifications can also be made, and these improvements and modifications can also be made. It should be regarded as the protection scope of the present invention.

Claims (11)

1. there is the compound nutritional mixed oil of ulcer repair it is characterised in that it by Abelmoschus esculentus seed oil, Fructus Cannabis oil and Auxiliary line of oils becomes.

2. the compound nutritional mixed oil with ulcer repair according to claim 1 is it is characterised in that described is auxiliary Oil is one of Oleum Arachidis hypogaeae semen, Oleum sesami, soybean oil, Semen Maydis oil, Oleum Brassicae campestriss, olive oil, Oleum Helianthi, Semen Lini oil or many Kind.

3. the compound nutritional mixed oil with ulcer repair according to claim 1 is it is characterised in that described is auxiliary Oil is soybean oil.

4. the compound nutritional mixed oil with ulcer repair according to any one of claims 1 to 3, its feature exists In total percentage composition of Abelmoschus esculentus seed oil and Fructus Cannabis oil is 40～95%, and the percentage composition of auxiliary oil is 5～60%.

5. the compound nutritional mixed oil with ulcer repair according to claim 4 is it is characterised in that Abelmoschus esculentus kind The weight of seed oil and Fructus Cannabis oil is than for 1:9～9:1.

6. the compound nutritional mixed oil with ulcer repair according to claim 1 is it is characterised in that it is by percentage Content is 48% Abelmoschus esculentus oil, and percentage composition is 32% Fructus Cannabis oil, and percentage composition is 20% soybean oil composition.

7. the preparation method of the compound nutritional mixed oil with ulcer repair described in any one of claim 1～6, it is special Levy and be, comprise the following steps:

(1) take Abelmoschus esculentus dry seed, pulverize, then adopt cold-press, cold-maceration, solvent extraction or co₂Supercritical fluid extracts Take, obtain Abelmoschus esculentus seed oil, standby；

(2) take band shell Urtica cannabina L. seed, vacuum drying, pulverize, sieve, then adopt cold-press, cold-maceration, solvent extraction or co₂Surpass and face Boundary's fluid extraction, prepares hemp seed oil, standby；

(3) hemp seed oil that the Abelmoschus esculentus seed oil preparing step (1) and step (2) prepare is mixed all with auxiliary oil Even, standing, filtration, fill, packaging.

8. the preparation method of the compound nutritional mixed oil with ulcer repair described in claim 7 is it is characterised in that institute The auxiliary oil stated is one of Oleum Arachidis hypogaeae semen, Oleum sesami, soybean oil, Semen Maydis oil, Oleum Brassicae campestriss, olive oil, Oleum Helianthi, Semen Lini oil Or it is multiple.

9. the preparation method of the compound nutritional mixed oil with preventing and treating acute gastric ulcer effect described in claim 7, its feature It is, the co of step (1) Abelmoschus esculentus seed oil₂Supercritical fluid extraction condition is: extracting pressure be 30mpa, temperature be 45～ 50 DEG C, extraction time be 90～180 minutes, carbon dioxide is 200～250kg/h by the flow of extraction kettle；Hemp seed oil Co₂Supercritical fluid extraction condition is: co₂Flow velocity 15-25l/h, 25～45 DEG C of extraction temperature, the extraction in front 0.5-1.0h Pressure is 15～25mpa, and then extracting pressure is increased to 25～35mpa, continues extraction 1.0～2.0h.

10. the compound nutritional mixed oil with ulcer repair described in any one of claim 1 to 6 prevents and treats skin in preparation With the application in the medicine of mucosa ulcer and health food.

The compound nutritional mixed oil of 11. present invention also can be combined with pharmaceutically acceptable carrier, according to conventional pharmaceutical methods Make soft capsule, membrane or ointment.