CN106349315B - A kind of Momordica grosvenori extracting method - Google Patents
A kind of Momordica grosvenori extracting method Download PDFInfo
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- CN106349315B CN106349315B CN201510411312.6A CN201510411312A CN106349315B CN 106349315 B CN106349315 B CN 106349315B CN 201510411312 A CN201510411312 A CN 201510411312A CN 106349315 B CN106349315 B CN 106349315B
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- momordica grosvenori
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- 241001409321 Siraitia grosvenorii Species 0.000 title claims abstract description 25
- 235000011171 Thladiantha grosvenorii Nutrition 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 11
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 26
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000007788 liquid Substances 0.000 claims abstract description 20
- 235000015203 fruit juice Nutrition 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 108010059892 Cellulase Proteins 0.000 claims abstract description 10
- 229940106157 cellulase Drugs 0.000 claims abstract description 10
- 239000006228 supernatant Substances 0.000 claims abstract description 10
- 239000004382 Amylase Substances 0.000 claims abstract description 9
- 102000013142 Amylases Human genes 0.000 claims abstract description 9
- 108010065511 Amylases Proteins 0.000 claims abstract description 9
- 235000019418 amylase Nutrition 0.000 claims abstract description 9
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 8
- 238000001556 precipitation Methods 0.000 claims abstract description 8
- 239000000284 extract Substances 0.000 claims abstract description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 5
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 5
- 238000001816 cooling Methods 0.000 claims abstract description 5
- 238000009928 pasteurization Methods 0.000 claims abstract description 5
- -1 pectase 3ml Proteins 0.000 claims abstract description 5
- 230000008961 swelling Effects 0.000 claims abstract description 5
- 239000000843 powder Substances 0.000 claims abstract description 4
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 229940088598 enzyme Drugs 0.000 claims description 6
- 238000009413 insulation Methods 0.000 claims 1
- 239000013049 sediment Substances 0.000 claims 1
- 229930182470 glycoside Natural products 0.000 abstract description 14
- 150000002338 glycosides Chemical class 0.000 abstract description 14
- 238000000605 extraction Methods 0.000 abstract description 11
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 3
- 229930195725 Mannitol Natural products 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 239000000594 mannitol Substances 0.000 description 3
- 235000010355 mannitol Nutrition 0.000 description 3
- 239000001814 pectin Substances 0.000 description 3
- 235000010987 pectin Nutrition 0.000 description 3
- 229920001277 pectin Polymers 0.000 description 3
- 206010011224 Cough Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
Landscapes
- Seasonings (AREA)
- Preparation Of Fruits And Vegetables (AREA)
- Non-Alcoholic Beverages (AREA)
- Medicines Containing Plant Substances (AREA)
- Jellies, Jams, And Syrups (AREA)
Abstract
The present invention proposes a kind of Momordica grosvenori extracting method, it is characterised in that comprises the following steps：(1) fresh fructus momordicae or dry fruit swelling are screened, screw press goes out fruit juice, and carries out pasteurization, then adds pectase, amylase and cellulase, pectase 3ml, amylase 6ml, cellulase 8ml are added in every liter of fruit juice liquid, being incubated 2 hours at 30 40 DEG C is digested；(2) fruit juice that step (1) obtains is concentrated into specific density as 1.2 in vacuum concentrator；(3) adding 90% 95% ethanol makes alcohol content be 65%, staticly settles, extracts supernatant, be concentrated into no alcohol taste, specific density 1.2；(4) liquid for obtaining step (3) adds pure water, and adds proper amount of active carbon, boils 20min, and cooling down simultaneously filters, and obtains water supernatant liquid, is concentrated into specific density as 1.2；(5) acetone is added in the liquid that step (4) obtains, acetone concentration (V/V) is reached 50 75%, separate out precipitation；(6) precipitation for obtaining step (5) adds pure water, and carries out high vacuum concentration to remove acetone, obtains stiff paste, is finally dried to obtain powder.The extraction of momordica grosvenori glycoside V is carried out using this method, the momordica grosvenori glycoside V high income of extraction, purity is high, quality is good.
Description
Technical field
The present invention relates to a kind of Momordica grosvenori extracting method.
Background technology
Utilization to Momordica grosvenori at present is mainly used in extracting momordica grosvenori glycoside V, and momordica grosvenori glycoside V is used for sweetener, and is used to stop
The mannitol of cough effect is not utilized well.Momordica grosvenori glycoside V content highest in Lo Han Guo fruit, and Momordica grosvenori
Glycosides V and mannitol also have certain content in fruit, and convenient material drawing is widely distributed, cheap.Thus, Momordica grosvenori is carried out
Comprehensive deep processing means a great, and explores from Lo Han Guo fruit while extracts momordica grosvenori glycoside V and mannitol, its meaning is quite remote
Greatly.
But the extraction process of current momordica grosvenori glycoside V is complicated, the purity of resulting momordica grosvenori glycoside V is not high.
The content of the invention
The present invention proposes a kind of Momordica grosvenori extracting method, and the extracting method technique is simple, and extraction momordica grosvenori glycoside V purity is high,
More than 70% can be reached, and quality is good.
The technical proposal of the invention is realized in this way：A kind of Momordica grosvenori extraction process, comprises the following steps：
(1) fresh fructus momordicae or dry fruit swelling are screened, screw press goes out fruit juice, and carries out pasteurization, then adds pectin
Enzyme, amylase and cellulase, pectase 3ml, amylase 6ml, cellulase 8ml are added in every liter of fruit juice liquid, in 30-
2 hours are incubated at 40 DEG C to be digested, the activity of wherein enzyme is 20u/mg；
(2) fruit juice that step (1) obtains is concentrated into specific density as 1.2 in vacuum concentrator；
(3) adding 90%-95% ethanol makes alcohol content be 65%, staticly settles, extracts supernatant, be concentrated into no alcohol taste,
Specific density is 1.2；
(4) liquid for obtaining step (3) adds pure water, and adds proper amount of active carbon, boils 20min, cooling down is simultaneously
Filtering, obtains water supernatant liquid, is concentrated into specific density as 1.2.
(5) acetone is added in the liquid that step (4) obtains, acetone concentration (V/V) is reached 50-75%, separate out precipitation；
(6) precipitation for obtaining step (5) adds pure water, and carries out high vacuum concentration to remove acetone, obtains stiff paste, most
After carry out being dried to obtain powder.
The beneficial effects of the invention are as follows：The extraction of momordica grosvenori glycoside V, the momordica grosvenori glycoside V purity of extraction are carried out using this method
Height, quality are good.
Embodiment
The technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described implementation
Example only part of the embodiment of the present invention, rather than whole embodiments.It is common based on the embodiment in the present invention, this area
The every other embodiment that technical staff is obtained under the premise of creative work is not made, belong to the model that the present invention protects
Enclose.
Embodiment 1：
A kind of Momordica grosvenori extraction process, comprises the following steps：
(1) fresh fructus momordicae or dry fruit swelling are screened, screw press goes out fruit juice, and carries out pasteurization, then adds pectin
Enzyme, amylase and cellulase, pectase 3ml, amylase 6ml, cellulase 8ml are added in every liter of fruit juice liquid, in 30-
2 hours are incubated at 40 DEG C to be digested, the activity of wherein enzyme is 20u/mg；
(2) fruit juice for obtaining step 1 is concentrated into specific density as 1.2 in vacuum concentrator；
(3) adding 90% ethanol makes alcohol content be 65%, staticly settles, extracts supernatant, be concentrated into no alcohol taste, specific density
For 1.2；
(4) liquid for obtaining step (3) adds pure water, and adds proper amount of active carbon, boils 20min, cooling down is simultaneously
Filtering, obtains water supernatant liquid, is concentrated into specific density as 1.2
(5) acetone is added in the liquid that step (4) obtains, acetone concentration (V/V) is reached 75%, separate out precipitation.
Embodiment 1：
A kind of Momordica grosvenori extraction process, comprises the following steps：
(1) fresh fructus momordicae or dry fruit swelling are screened, screw press goes out fruit juice, and carries out pasteurization, then adds pectin
Enzyme, amylase and cellulase, pectase 3ml, amylase 6ml, cellulase 8ml are added in every liter of fruit juice liquid, in 30-
2 hours are incubated at 40 DEG C to be digested；
(2) fruit juice for obtaining step 1 is concentrated into specific density as 1.2 in vacuum concentrator；
(3) adding 90% ethanol makes alcohol content be 65%, staticly settles, extracts supernatant, be concentrated into no alcohol taste, specific density
For 1.2；
(4) liquid for obtaining step (3) adds pure water, and adds proper amount of active carbon, boils 20min, cooling down is simultaneously
Filtering, obtains water supernatant liquid, is concentrated into specific density as 1.2
(5) acetone is added in the liquid that step (4) obtains, acetone concentration (V/V) is reached 65%, separate out precipitation.
The extraction of momordica grosvenori glycoside V is carried out using this method, the momordica grosvenori glycoside V purity of extraction is high, and quality is good.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
God any modification, equivalent substitution and improvements made etc., should be included in the scope of the protection with principle.
Claims (1)
1. a kind of Momordica grosvenori extracting method, it is characterised in that comprise the following steps：
(1) fresh fructus momordicae or dry fruit swelling are screened, screw press goes out fruit juice, and carries out pasteurization, then adds pectase, forms sediment
Powder enzyme and cellulase, pectase 3ml, amylase 6ml, cellulase 8ml are added in every liter of fruit juice liquid, at 30-40 DEG C
Insulation is digested for 2 hours；
(2) fruit juice that step (1) obtains is concentrated into specific density as 1.2 in vacuum concentrator；
(3) adding 90%-95% ethanol makes alcohol content be 65%, staticly settles, extracts supernatant, no alcohol taste is concentrated into, than close
Spend for 1.15；
(4) liquid for obtaining step (3) adds pure water, and adds proper amount of active carbon, boils 20min, and cooling down simultaneously filters,
Water supernatant liquid is obtained, is concentrated into specific density as 1.2
(5) acetone is added in the liquid that step (4) obtains, acetone concentration (V/V) is reached 50-75%, separate out precipitation；
(6) precipitation for obtaining step (5) adds pure water, and carries out high vacuum concentration to remove acetone, obtains stiff paste, most laggard
Row is dried to obtain powder.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510411312.6A CN106349315B (en) | 2015-07-14 | 2015-07-14 | A kind of Momordica grosvenori extracting method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510411312.6A CN106349315B (en) | 2015-07-14 | 2015-07-14 | A kind of Momordica grosvenori extracting method |
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| Publication Number | Publication Date |
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| CN106349315A CN106349315A (en) | 2017-01-25 |
| CN106349315B true CN106349315B (en) | 2017-12-01 |
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| CN108433054A (en) * | 2018-03-09 | 2018-08-24 | 合浦穗香食品有限公司 | A kind of pastry food additive and application |
| CN108635442A (en) * | 2018-05-18 | 2018-10-12 | 双柏燚天商贸有限公司 | A kind of finger citron sliced formula and processing technology |
| CN108651662A (en) * | 2018-05-18 | 2018-10-16 | 双柏燚天商贸有限公司 | A kind of fingered citron instant tea formula and processing technology |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101200753A (en) * | 2007-08-09 | 2008-06-18 | 桂林惠通生物科技有限公司 | Fructus momordicae extract with mogroside V content being more than or equal to 40% and preparation method thereof |
| CN102675398A (en) * | 2012-05-27 | 2012-09-19 | 唐忠海 | Method for extracting momordica grosvenori glycoside V and farnesol from grosvenor momordica fruits |
| CN102742906A (en) * | 2012-07-18 | 2012-10-24 | 何伟平 | Momordica grosvenori drink with low glycemic index and anti-fatigue action |
-
2015
- 2015-07-14 CN CN201510411312.6A patent/CN106349315B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101200753A (en) * | 2007-08-09 | 2008-06-18 | 桂林惠通生物科技有限公司 | Fructus momordicae extract with mogroside V content being more than or equal to 40% and preparation method thereof |
| CN102675398A (en) * | 2012-05-27 | 2012-09-19 | 唐忠海 | Method for extracting momordica grosvenori glycoside V and farnesol from grosvenor momordica fruits |
| CN102742906A (en) * | 2012-07-18 | 2012-10-24 | 何伟平 | Momordica grosvenori drink with low glycemic index and anti-fatigue action |
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| CN106349315A (en) | 2017-01-25 |
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Denomination of invention: An extraction method of Siraitia grosvenorii Effective date of registration: 20201207 Granted publication date: 20171201 Pledgee: Xiushan Industrial Development Investment Co.,Ltd. Pledgor: CHONGQING WANWU CHUNSHENG PHARMACEUTICAL Co.,Ltd. Registration number: Y2020500000022 |
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Date of cancellation: 20220222 Granted publication date: 20171201 Pledgee: Xiushan Industrial Development Investment Co.,Ltd. Pledgor: CHONGQING WANWU CHUNSHENG PHARMACEUTICAL Co.,Ltd. Registration number: Y2020500000022 |
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Effective date of registration: 20220309 Address after: 409900 No. 6, Wuyang Avenue, Wuyang street, Qianjiang District, Chongqing Patentee after: CHONGQING GUOTAI KANGNING PHARMACEUTICAL Co.,Ltd. Address before: 409900 LU1, park, Wuyang street, Xiushan Tujia and Miao Autonomous County, Chongqing Patentee before: CHONGQING WANWU CHUNSHENG PHARMACEUTICAL Co.,Ltd. |
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Address after: 409900 No. 6, Wuyang Avenue, Wuyang street, Xiushan County, Chongqing Patentee after: CHONGQING GUOTAI KANGNING PHARMACEUTICAL Co.,Ltd. Address before: 409900 No. 6, Wuyang Avenue, Wuyang street, Qianjiang District, Chongqing Patentee before: CHONGQING GUOTAI KANGNING PHARMACEUTICAL Co.,Ltd. |
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