CN106349136A - Method for extracting zeaxanthin and derivative of zeaxanthin from wolfberry - Google Patents
Method for extracting zeaxanthin and derivative of zeaxanthin from wolfberry Download PDFInfo
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- CN106349136A CN106349136A CN201610768879.3A CN201610768879A CN106349136A CN 106349136 A CN106349136 A CN 106349136A CN 201610768879 A CN201610768879 A CN 201610768879A CN 106349136 A CN106349136 A CN 106349136A
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- fructus lycii
- cryptoxanthin
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C403/00—Derivatives of cyclohexane or of a cyclohexene or of cyclohexadiene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene or cyclohexadiene rings, e.g. vitamin A, beta-carotene, beta-ionone
- C07C403/24—Derivatives of cyclohexane or of a cyclohexene or of cyclohexadiene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene or cyclohexadiene rings, e.g. vitamin A, beta-carotene, beta-ionone having side-chains substituted by six-membered non-aromatic rings, e.g. beta-carotene
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
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- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
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Abstract
The invention relates to a method for extracting active substances from a wolfberry, in particular to a method for extracting zeaxanthin and a derivative of the zeaxanthin from the wolfberry. The method comprises the steps of performing sugar reduction on the wolfberry, drying 10 kg wolfberry, grinding into 100 meshes, putting the wolfberry in a supercritical extraction kettle for extraction, merging and collecting an extract for 0.6-3 h to obtain the zeaxanthin and the derivative of the zeaxanthin. The method does not use an organic solvent in the whole process, and is safe and efficient, technical parameters are applicable to pilot-plant-scale production, and the purity reaches 80% or above.
Description
Technical field
The present invention relates to a kind of method extracting active substance in Fructus Lycii, especially relate to a kind of Semen Maydiss extracting in Fructus Lycii
Yellow matter and its method for derivant.
Background technology
Cryptoxanthin belongs to oxygen-containing carotenoid, and molecular formula is c40h58o2, it is primarily present in bottle green vegetable, egg yolk, jade
In rice, citrus fruit etc., human body can not be directly synthesized.Cryptoxanthin is widely distributed in the tissue such as human serum, liver, its
Middle macula retinae Midst density highest, they are to stop light from exposing the most effective composition causing retina injury.Cryptoxanthin enters
It is protein mediated by particular combination for entering retina.At present, age-related macular degeneration and cataract are to lead to inpairment of vision very
To blind several frequently seen ocular disease, the research of existing a lot of cryptoxanthin and cataract relation aspect both at home and abroad.Increasingly
Many epidemiology and clinical trial show, in meals or serum, the cryptoxanthin of high level can reduce ocular disease illness wind
Danger；Fructus Lycii is traditional medicine-food two-purpose resource.Medlar health preserving historical origin for a long time, is nowadays more subject to the height weight of experts and scholars
Depending on.The outstanding written Compendium of Material Medica of medical scholar's Li Shizhen (1518-1593 A.D.) of the Ming Dynasty is recorded: " Fructus Lycii, kidney-tonifying sperm-generating, nourishing the liver, improving eyesight, hard essence
Bone, fatigue taking away, easy color, bleach, improving eyesight is calmed the nerves, make us long-lived ".Modern medicine shows, is rich in main improving eyesight effect in Fructus Lycii
Composition carotenoid material, is 4-20 times of carotenoid in common fruit and vegerable, and predominantly cryptoxanthin and its derivant, possesses
Single medicinal material, the condition of functional health care product deep level development.
Cryptoxanthin and its derivant belong to fat-soluble chemical substance, and its extraction process is presently mainly solvent method.Conventional
Solvent have hexane, acetone, petroleum ether and oxolane etc., these solvents are respectively provided with different degrees of toxicity.Therefore, tradition
Solvent extraction method because of factors such as its solvent toxicity residuals, the extraction limiting zeaxanthin separates and its further deep processing
Using；Supercritical co2Abstraction technique extracts the main research that natural carotenoid is supercritical liquid extraction technique and application neck
One of domain, and it is increasingly becoming the focus of domestic and international research and development.But it is subject to Fructus Lycii sugared content, water content and seed oil content relatively at present
High factor impact, technique is rarely seen in the extraction application of Fructus Lycii cryptoxanthin and its derivant.
Content of the invention
The purpose of the present invention is to overcome the deficiencies in the prior art, provides a kind of overall process organic solvent-free, safe efficient,
And this technical parameter be adapted to enlarged experiment produce and the purity cryptoxanthin that reaches in a kind of more than 80% extraction Fructus Lycii and its
The method of derivant.
The present invention is accomplished in that
A kind of extract Fructus Lycii in cryptoxanthin and its derivant method it is characterised in that: the method be first by Fructus Lycii
Blood sugar lowering, then takes 10kg to be crushed to 100 mesh after drying, is placed in supercritical extraction reactor and is extracted, be then combined with collecting 0.6h-
3h extract, is gained cryptoxanthin and its derivant；
Described extraction conditionss are: extraction temperature 35-45 DEG C, extracting pressure are 35-45mpa, co2Flow 300-370l/h,
Extraction time is 3 hours；
The process of described Fructus Lycii blood sugar lowering is that the Fructus Lycii of remove impurity is placed in 50 DEG C of water of 6-10 times of quality, stands 1 hour,
It is repeated 3 times, after dewatering, room temperature is dried or 40-50 DEG C of hot-air seasoning, so that Fructus Lycii raw material total sugar content is reduced to from 41%-45%
25%-29%, water content is reduced to 10%-8% from 11%-12%；
Described extraction quantity is 2-6kg/100kg, analyzes detection through hplc, and gained extract is mainly zeaxanthin two Petiolus Trachycarpi
Acid esters, zeaxanthin monopalmitate, cryptoxanthin, kryptoxanthin and beta-carotene, wherein, zeaxanthin dipalmitate contains
Amount 80%；
Described Fructus Lycii is fresh fructus lycii or dry wolfberry.
The present invention has the effect that
1) method is unique: the method that the present invention provides is that the Fructus Lycii of remove impurity is placed in 50 DEG C of water of 6-10 times of quality,
Standing 1 hour, is repeated 3 times, after dewatering, room temperature is dried or 40-50 DEG C of hot-air seasoning.Make Fructus Lycii raw material total sugar content from 41%-
45% is reduced to 25%-29%, and water content is reduced to 10%-8% from 11%-12%.Fructus Lycii after taking 10kg to dry, is crushed to
100m mesh, is placed in supercritical extraction reactor and is extracted.Extraction conditionss are: extraction temperature 35-45 DEG C, extracting pressure are 35-
45mpa、co2Flow 300-370l/h, extraction time is 3 hours.Wherein, merge and collect 0.6h-3h extract, be gained Semen Maydiss
Yellow matter and its derivant.The method with Fructus Lycii as raw material, using supercritical co2Abstraction technique extraction is obtained purity and is more than 75%
Fructus Lycii cryptoxanthin and its derivant.
2) extraction process is safe efficient: the present invention extract obtained more than 80% is cryptoxanthin and its derivant, mainly
For zeaxanthin dipalmitate, after extracting raw material pre-treatment, extraction and extraction, overall process is all provided without organic solvent, safely,
Efficiently.
3), after the method pre-treatment that the present invention provides, solve enlarged experiment production extraction slag compacting and be difficult to take in extraction kettle
The bottleneck problem going out；
4) product extracting has improving eyesight, alleviates the effects such as asthenopia, prevention cardiovascular and cerebrovascular disease: the side that the present invention provides
Material residue after method extraction is easy to the extraction of lycium barbarum polysaccharide, and material residue low small molecular sugar content reduces 40%-50%.Through function test card
Bright, there is improving eyesight, alleviate the effects such as asthenopia, prevention cardiovascular and cerebrovascular disease.Product can be directly used for healthy food material, processing
Prepare various health foods and medicine.
Specific embodiment
Embodiment one: a kind of method of cryptoxanthin extracting in Fructus Lycii and its derivant, the method is by the Chinese holly of remove impurity
Fructus Lycii is placed in 50 DEG C of water of 6-10 times of quality, stands 1 hour, is repeated 3 times, after dewatering, room temperature is dried or 40 DEG C of hot-air seasonings.
Fructus Lycii raw material total sugar content is made to be reduced to 25%-29% from 41%-45%, water content is reduced to 10%-8% from 11%-12%,
Fructus Lycii after taking 10kg to dry, is crushed to 100m mesh, is placed in supercritical extraction reactor and is extracted.Extraction conditionss are: extraction temperature
35 DEG C, extracting pressure be 35mpa, co2 flow 300l/h, extraction time be 3 hours, wherein, merge collect 0.6h-3h extraction
Thing, is gained cryptoxanthin and its derivant.Extraction quantity is 2-6kg/100kg, analyzes detection through hplc, and gained extract is main
For zeaxanthin dipalmitate, zeaxanthin monopalmitate, cryptoxanthin, kryptoxanthin and beta-carotene.Wherein, Semen Maydiss
Flavin dipalmitate content 80%.In above-mentioned cranberry extract, the weight/mass percentage composition of anthocyanin is 10%.
Embodiment two: a kind of method of cryptoxanthin extracting in Fructus Lycii and its derivant, the method is by the Chinese holly of remove impurity
Fructus Lycii is placed in 50 DEG C of water of 6-10 times of quality, stands 1 hour, is repeated 3 times, after dewatering, room temperature is dried or 50 DEG C of hot-air seasonings.
Fructus Lycii raw material total sugar content is made to be reduced to 25%-29% from 41%-45%, water content is reduced to 10%-8% from 11%-12%.
Fructus Lycii after taking 10kg to dry, is crushed to 100m mesh, is placed in supercritical extraction reactor and is extracted.Extraction conditionss are: extraction temperature
45 DEG C, extracting pressure be 45mpa, co2Flow 370l/h, extraction time is 3 hours, wherein, merges and collects 0.6h-3h extraction
Thing, is gained cryptoxanthin and its derivant.Extraction quantity is 2-6kg/100kg, analyzes detection through hplc, and gained extract is main
For zeaxanthin dipalmitate, zeaxanthin monopalmitate, cryptoxanthin, kryptoxanthin and beta-carotene.Wherein, Semen Maydiss
Flavin dipalmitate content 80%.In above-mentioned cranberry extract, the weight/mass percentage composition of anthocyanin is 10%.
Embodiment three: a kind of method of cryptoxanthin extracting in Fructus Lycii and its derivant, the method is by the Chinese holly of remove impurity
Fructus Lycii is placed in 50 DEG C of water of 6-10 times of quality, stands 1 hour, is repeated 3 times, after dewatering, room temperature is dried or 45 DEG C of hot-air seasonings.
Fructus Lycii raw material total sugar content is made to be reduced to 25%-29% from 41%-45%, water content is reduced to 10%-8% from 11%-12%.
Fructus Lycii after taking 10kg to dry, is crushed to 100m mesh, is placed in supercritical extraction reactor and is extracted.Extraction conditionss are: extraction temperature
40 DEG C, extracting pressure be 40mpa, co2Flow 350l/h, extraction time is 3 hours, wherein, merges and collects 0.6h-3h extraction
Thing, is gained cryptoxanthin and its derivant.Extraction quantity is 2-6kg/100kg, analyzes detection through hplc, and gained extract is main
For zeaxanthin dipalmitate, zeaxanthin monopalmitate, cryptoxanthin, kryptoxanthin and beta-carotene.Wherein, Semen Maydiss
Flavin dipalmitate content 80%.In above-mentioned cranberry extract, the weight/mass percentage composition of anthocyanin is 10%.
Described Fructus Lycii is fresh fructus lycii or dry wolfberry.
Claims (5)
1. a kind of extract Fructus Lycii in cryptoxanthin and its derivant method it is characterised in that: the method be first Fructus Lycii is dropped
Sugar, then takes 10kg to be crushed to 100 mesh after drying, is placed in supercritical extraction reactor and is extracted, be then combined with collecting 0.6h-3h
Extract, is gained cryptoxanthin and its derivant.
2. as claimed in claim 1 a kind of extract Fructus Lycii in cryptoxanthin and its derivant method it is characterised in that: institute
Stating extraction conditionss is: extraction temperature 35-45 DEG C, extracting pressure are 35-45mpa, co2Flow 300-370l/h, extraction time is 3
Hour.
3. as claimed in claim 1 a kind of extract Fructus Lycii in cryptoxanthin and its derivant method it is characterised in that: institute
The process stating Fructus Lycii blood sugar lowering is that the Fructus Lycii of remove impurity is placed in 50 DEG C of water of 6-10 times of quality, stands 1 hour, is repeated 3 times, drip
After water, room temperature is dried or 40-50 DEG C of hot-air seasoning, makes Fructus Lycii raw material total sugar content be reduced to 25%-29%, water content from 41%-45%
It is reduced to 10%-8% from 11%-12%.
4. as claimed in claim 1 a kind of extract Fructus Lycii in cryptoxanthin and its derivant method it is characterised in that: institute
Stating extraction quantity is 2-6kg/100kg, analyzes detection through hplc, gained extract is mainly zeaxanthin dipalmitate, Semen Maydiss
Flavin monopalmitate, cryptoxanthin, kryptoxanthin and beta-carotene, wherein, zeaxanthin dipalmitate content 80%.
5. as claimed in claim 1 a kind of extract Fructus Lycii in cryptoxanthin and its derivant method it is characterised in that: institute
Stating Fructus Lycii is fresh fructus lycii or dry wolfberry.
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| CN201610768879.3A CN106349136B (en) | 2016-08-30 | 2016-08-30 | A method of zeaxanthin and its derivative in extraction matrimony vine |
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| CN201610768879.3A CN106349136B (en) | 2016-08-30 | 2016-08-30 | A method of zeaxanthin and its derivative in extraction matrimony vine |
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Cited By (4)
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|---|---|---|---|---|
| CN108047110A (en) * | 2017-12-11 | 2018-05-18 | 宁夏农林科学院枸杞工程技术研究所 | A kind of method that luteole acid dipalmitate is prepared using matrimony vine as raw material |
| CN111772091A (en) * | 2020-07-29 | 2020-10-16 | 毛忠英 | Method for extracting lycium barbarum pigment by using supercritical carbon dioxide and raw material pretreatment method |
| CN112075524A (en) * | 2020-09-27 | 2020-12-15 | 宁夏农林科学院枸杞工程技术研究所 | Wolfberry glycoprotein and zeaxanthin ester composite candy tablet |
| CN112255132A (en) * | 2020-11-25 | 2021-01-22 | 宁夏农林科学院枸杞工程技术研究所 | Method for determining hardness of medlar fruits based on texture analyzer |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108047110A (en) * | 2017-12-11 | 2018-05-18 | 宁夏农林科学院枸杞工程技术研究所 | A kind of method that luteole acid dipalmitate is prepared using matrimony vine as raw material |
| CN111772091A (en) * | 2020-07-29 | 2020-10-16 | 毛忠英 | Method for extracting lycium barbarum pigment by using supercritical carbon dioxide and raw material pretreatment method |
| CN112075524A (en) * | 2020-09-27 | 2020-12-15 | 宁夏农林科学院枸杞工程技术研究所 | Wolfberry glycoprotein and zeaxanthin ester composite candy tablet |
| CN112255132A (en) * | 2020-11-25 | 2021-01-22 | 宁夏农林科学院枸杞工程技术研究所 | Method for determining hardness of medlar fruits based on texture analyzer |
| CN112255132B (en) * | 2020-11-25 | 2023-07-28 | 宁夏农林科学院枸杞工程技术研究所 | Method for measuring hardness of wolfberry fruits based on texture analyzer |
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