CN106342671A - Method for cultivating healthy sugarcane plants - Google Patents
Method for cultivating healthy sugarcane plants Download PDFInfo
- Publication number
- CN106342671A CN106342671A CN201610767067.7A CN201610767067A CN106342671A CN 106342671 A CN106342671 A CN 106342671A CN 201610767067 A CN201610767067 A CN 201610767067A CN 106342671 A CN106342671 A CN 106342671A
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- pesticide
- seedling
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- healthy
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
- A01G13/10—Devices for affording protection against animals, birds or other pests
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The invention provides a method for cultivating healthy sugarcane plants. The method includes arranging healthy sugarcane seed stems or seedlings in healthy sugarcane plant cultivating devices; arranging the healthy sugarcane plant cultivating devices in seedling growing greenhouses with insect-proof nets; cultivating plants in a soilless manner; spraying nutrient liquid at regular time; spraying pesticide mixtures at fixed periods and cultivating the plants to obtain seed stems which can be used as donors for breeding healthy tissue cultivation seedlings. The method has the advantages that the bred sugarcane plants are the healthy plants; the seed stems can be used as the donors for breeding the healthy tissue cultivation seedlings, accordingly, healthy tissue cultivation seedling production programs can be simplified, the detection quantities of tissue cultivation seedling pathogens can be reduced, and the detection cost can be lowered; the stem tip induction differentiation survival rate can be increased, the tissue cultivation seedling breeding base numbers can be increased, and accordingly the seedling growing efficiency can be greatly improved.
Description
Technical field
The invention belongs to arable farming and prevention and control of plant diseases, pest control field, it is related to a kind of method cultivating sugarcane health plant.
Background technology
Caulis Sacchari sinensis are the main sugar crops of China, and cultivated area accounts for China's sugar crop gross area 90% about, and sucrose produces
Liang Zhan China sugar yield more than 90%.
Cane seedling produce traditionally carried out using asexual propagation, breeding coefficient is low, for new varieties Rapid Popularization not
Profit;Additionally, Caulis Sacchari sinensis are in process of production, repeatedly infected by multiple source of disease thing, the more disease of stem accumulation is planted in plantation for many years afterwards
Source bacterium, part source of disease bacterium applied chemistry medicine cannot remove, and suppresses sugarcane production, cause sugarcane yield and quality significantly under
Fall.
Sugarcane health seedling technology is to breed the seedling of acquisition by shoot apical meristem binding molecule detection technique, can go
Except the source of disease bacterium of accumulation on kind of stem, recover the good strains of seeds of breeding, improve sugarcane yield and sugar;Application health seedling technology is numerous
Educate improved Varieties, its breeding coefficient can improve more than 1000 times than tradition, its popularization and application speed can be increased.
Sugarcane health seedling technology breeds the Molecular Detection that one of its key technology of health seedling is source of disease thing.In order to determine
The seedling health no pathogen being obtained, need to detect to each stem apex strain Seedling, in addition for reducing exogenous hormone to tissue cultured seedling
Produce the effect of variation, general culture algebraically is less than 8-10 generation.Accordingly, it would be desirable to substantial amounts of kind of stem donor, in current detection skill
Under conditions of art is more complicated, detection workload is big, time length, high cost, and the popularization to health seedling technology is very unfavorable.
If Caulis Sacchari sinensis plant is anosis source thing, the sugarcane tissue-culture bottle seedling that the shoot apical meristem of its kind of stem lateral bud is bred is applied then may be used
It is defined as no source of disease thing, be health seedling.
Content of the invention
In order to solve above technical problem, it is an object of the invention to provide a kind of method cultivating sugarcane health plant.
The method cultivating sugarcane health plant of the present invention, step is: sugarcane health kind stem or seedling is placed in sweet
In sugarcane healthy plant breeding apparatus, and described healthy plant breeding apparatus are placed in fly net seedling raising greenhouse, plant is carried out
Soilless culture, timing spray nutritious liquor, regular spraying pesticide mixture, cultivating the kind stem obtaining can be used as breeding healthy tissue-cultured Seedling
Donor.
Cultivate the kind stem obtaining and produce healthy tissue-cultured Seedling, before Caulis Sacchari sinensis cut receipts inoculation, to each bud system plant detection one
Secondary pathogen is it is ensured that health seedling donor health is anosis;The health seedling bred is produced by stem apex Virus-free seedcane program, stem
Sharp separate living tissue can be carried out the normal proliferative stage after obtaining tissue cultured seedling, is not required to by detecting the presence of pathogen.
Preferably, described sugarcane health kind stem or seedling, is the Caulis Sacchari sinensis kind not carrying any pathogen through pcr Molecular Detection
Stem or seedling.
Preferably, every liter of described nutritional solution includes potassium nitrate 900~1000mg, calcium ammonium nitrate 750~850mg, magnesium sulfate
350~400mg, potassium dihydrogen phosphate 170~200mg, ethylenediaminetetraacetic acid 35~40mg, ferrous sulfate 53~56mg, boric acid 8~
8.5mg, manganese sulfate 20~25mg, zinc sulfate 1.5~1.8mg, copper sulfate 0.03~0.06mg, sodium molybdate 0.01~0.02mg.
Preferably, described pesticide combination includes insecticide pesticide and sterilization pesticide, and described insecticide pesticide includes pesticide a, agriculture
Medicine b and pesticide c, described sterilization pesticide includes pesticide d and pesticide e, and its concentration is:
The gamma cyhalothrin aqueous emulsion that described pesticide a is 5% for mass concentration, concentration is 20~30ml/l;
The Rynaxypyr suspending agent that described pesticide b is 20% for mass concentration, concentration is 1~2ml/l;
Described pesticide c is 25% Diacloden water dispersible granules for mass concentration, and concentration is 1~2 gram/l;
The benzene first propiconazole suspending agent that described pesticide d is 30% for mass concentration, concentration is 0.4~1.0ml/l;
The S-Ethyl ethylthio sulfonate cream that described pesticide e is 80% for mass concentration, concentration is 0.12~0.20ml/l.
Preferably, described sugarcane health plant cultivating device includes cultivating bucket, cultivates bucket two side perpendicular to the ground
Spud pile, parallel to ground and the transverse support bar on spud pile and feed liquid conveyer device, be provided with described cultivation bucket bucket
Determine root groove, described determine root groove cultivation bottom of the barrel is supported in by carrier;Described medicinal liquid conveyer device is included located at cultivation bucket top
Shower nozzle, time switch, air compressor machine and storage tank, described shower nozzle is connected with storage tank by feeder sleeve.
Preferably, described cultivation bucket is Plastic Drum, and it pushes up as the bucket top with crimping, bucket top internal diameter 45cm, drum head internal diameter
35cm, the high 55cm of bucket, are provided with 2 osculums at drum head 10cm.
Preferably, spray nutritious liquor is supplied with mist state form, and the mode of spraying is: before seedling stage Caulis Sacchari sinensis root system cultivates drum head, often
Spray once within 10 minutes, 5 seconds every time;After Caulis Sacchari sinensis root system reaches cultivation drum head, daytime, 7:00-19:00, sprayed once, often for every 30 minutes
Secondary 10 seconds;Evening 19:00 to the next morning 7:00, sprays once per hour, 5 seconds every time.
Preferably, spraying pesticide mixture sprayed once for every 30 days, 10 seconds every time.
Preferably, the insect protected reticular density of described fly net seedling raising greenhouse is 120 mesh, canopy high 5-6 rice.
Advantages of the present invention is:
1st, space is cultivated for Caulis Sacchari sinensis with fly net booth, application is cultivated bucket and carried out soilless culture, pass through anti-in cultivation
Worm net, chemical pesticide etc. prevent pathogen to healthy sugarcane strain harm and re-infection, thus carrying for breeding health cane tissue cultured seedling
For healthy plant;
2nd, can carry out the normal proliferative stage in acquisition Shoot-tip Culture after seedling, pathogen need not be detected the presence of;Healthy tissue-cultured
Seedling production routine simplifies, and decreases the detection limit of tissue cultured seedling pathogen, reduces testing cost;
3rd, Caulis Sacchari sinensis are healthy plant, stem apex no pathogen, the peelable larger shoot apical meristem when stem apex strips, and stem apex lures
Lead differentiation and survive raising 50%-60%;
4th, pathogen need not be detected the presence of using the Shoot-tip Culture Seedling that this method obtains, make stem apex break up the initial Seedling of gained
It is completely used for breeding, tissue cultured seedling breeds radix increases 0.5-1.0 times, and breeding efficiency greatly improves.
Brief description
Fig. 1 cultivates the structural representation of Caulis Sacchari sinensis plant device for the present invention;
The drawing reference numeral of in figure is: 1- spud pile, and 2- cultivates bucket, 201- osculum, 202- crimping, and 3- determines root groove, and 4- holds
Carrier, 5- shower nozzle, 6- feeder sleeve, 7- first storage tank, 8- second storage tank, 9- the 3rd storage tank, 10- air compressor machine, 11- time switch,
12- location hook, 13- lanyard, 14- transverse support bar.
Specific embodiment
Below the preferably embodiment of the present invention is described in further detail:
Cultivate the method that sugarcane health plant breeds health seedling, step is as follows:
(1) choose sugarcane health kind stem, do not carry any pathogen through pcr Molecular Detection, take simple bud section.
(2) sand culture accelerating germination is bred as the seedling with 2-3 leaf Seedling.
(3) as shown in figure 1, cultivating bucket 2 to select the Plastic Drum with crimping 202, its mouthful of diameter 45cm, bottom diameter 35cm, height
55cm, sets 2 little osculums 201 away from drum head 10cm.Step (2) gained seedling is placed in cultivation bucket 2 and carries out soilless culture,
It is provided with one in cultivation bucket 2 and determines root groove 3, determine root groove 3 and cultivation bucket 5 bottom is supported in by carrier 4, away from drum head 30-40cm;Training
Educate bucket 2 top and dispose three shower nozzles 5, three shower nozzles 5 pass through three feeder sleeves 6 respectively with the first storage tank 7, the second storage tank the 8, the 3rd
Storage tank 9 connects, and three storage tanks store nutritional solution, insecticide pesticide admixing medical solutions and sterilization pesticide admixing medical solutions respectively;By shower nozzle 5,
Time switch 11, air compressor machine 10 and three tank batterys become medicinal liquid conveyer device;Cultivate also to stand around bucket 2 and have high 4.0-4.5 rice
Spud pile 1, it is anti-down to be used for fixing Caulis Sacchari sinensis plant, and spud pile 1 is additionally provided with some location hooks 12, and available lanyard 13 is around cultivating bucket
2 bead portion is fixed on cultivating bucket on spud pile 1.When Caulis Sacchari sinensis plant grows into certain altitude, plant is fixed on laterally
On support bar 14, prevent Caulis Sacchari sinensis plant from lodging occurring.With the growth of Caulis Sacchari sinensis plant, adjust transverse support bar 14 and be placed in different height
On the location hook 12 of degree, fix Caulis Sacchari sinensis plant further.Meanwhile, feeder sleeve 6 selects rubber hose, can be with the life of Caulis Sacchari sinensis plant
Long, flexible pipe is suspended on the location hook 12 of differing heights, realizes the regulation of shower nozzle 5 suspension height.Described sugarcane health plant
Breeding apparatus are placed in the seedling raising greenhouse comprising fly net and use, and described insect protected reticular density is 120 mesh, canopy high 5-6 rice.
(4) cultivating nutrient solution mist state form timing supplies, before 1. seedling stage Caulis Sacchari sinensis root system cultivates drum head, spray one in every 10 minutes
Secondary, 5 seconds every time;2., after Caulis Sacchari sinensis root system reaches Plastic Drum bottom, daytime, 7:00-19:00, sprayed once, 10 seconds every time for every 30 minutes;Evening
Upper 19:00 to the next morning 7:00, sprays once, 5 seconds every time for every 1 hour.
(5) cultivating nutrient solution, in every liter of nutritional solution contain potassium nitrate 950mg, calcium ammonium nitrate 800mg, magnesium sulfate 375mg,
Potassium dihydrogen phosphate 185mg, ethylenediaminetetraacetic acid 37.5mg, ferrous sulfate 54.4mg, boric acid 8.3mg, manganese sulfate 22.4mg, sulphuric acid
Zinc 1.62mg, copper sulfate 0.05mg, sodium molybdate 0.02mg, ph5.8-6.8.
(6) pesticide preparation: 1. prepare insecticide pesticide admixing medical solutions 1l, its compound method is as follows: first takes clean tap water 700
~800ml, then takes 5% gamma cyhalothrin aqueous emulsion 20~30ml to pour in above-mentioned water and is sufficiently mixed, then take 20%
Rynaxypyr suspending agent 1~2ml pour in above-mentioned water and be sufficiently mixed, take 25% Diacloden water dispersible granules to take 1~2 gram
Add in above-mentioned water and be sufficiently mixed, be eventually adding clean tap water and be settled to 1l;2. prepare sterilization pesticide admixing medical solutions 1l,
Its compound method is as follows: first take clean tap water 700~800ml, then take 30% benzene first propiconazole suspending agent 0.4~1ml,
Take 80% S-Ethyl ethylthio sulfonate cream 0.12~0.2ml again, add in above-mentioned water and be sufficiently mixed, be eventually adding the fixed molten 1l of clean tap water
Sterilization pesticide admixing medical solutions can be joined to obtain.
(7) pesticide periodically sprays, and the pesticide of preparation is carried out root system or foliage-spray to Caulis Sacchari sinensis Seedling, every 30 days once, often
Secondary 10 seconds.
(8) cultivate the kind stem obtaining and produce healthy tissue-cultured Seedling, before Caulis Sacchari sinensis cut receipts inoculation, to each bud system plant detection
Pathogen is it is ensured that health seedling donor health is anosis;The health seedling bred is produced by stem apex Virus-free seedcane program,
Shoot apical meristem can be carried out the normal proliferative stage after obtaining tissue cultured seedling, is not required to by detecting the presence of pathogen.
The kind stem taking this method gained strips stem apex, is used for breeding healthy tissue-cultured Seedling.
Conventional plant cultivating method is:
(1) plantation place is the land for growing field crops not isolating condition;
(2) the kind stem (Seedling) bred is conventional production kind;
(3) measure of sugarcane production process field management technology is identical with conventional Caulis Sacchari sinensis field planting;
(4) take conventional plant to carry out health seedling and breed implementation steps reference: Li Song, Yu Kunxing, Liu Limin, etc. Caulis Sacchari sinensis
Stem apex embryoid detoxic seedling fast breeding technique studies [j]. Chinese sugar material, 2010 (04): 1-5.
Using conventional plant as comparison (ck), Caulis Sacchari sinensis stem apex is survived with situation and sugarcane bud donor is bred 120 days and obtained tissue culture
Growth of cereal crop seedlings condition is counted, and result is respectively as shown in table 1 and table 2:
Table 1 stem apex survival rate information slip
2 200 sugarcane bud donors of table breed 120 days obtained tissue cultured seedling number information slips
Note: 1 stem apex obtains 1000 plants of meters of tissue cultured seedling number after breeding 120 days.
As can be seen here, the healthy plant cultivated using the present invention, stem apex no pathogen, the peelable larger stem when stem apex strips
Sharp separate living tissue, Stem tip induction differentiation survival rate improves 60%, and breeding efficiency greatly improves.
Above content is to further describe it is impossible to assert with reference to specific preferred implementation is made for the present invention
Being embodied as of the present invention is confined to these explanations.For general technical staff of the technical field of the invention,
On the premise of present inventive concept, some simple deduction or replace can also be made, all should be considered as belonging to the present invention's
Protection domain.
Claims (9)
1. a kind of method cultivating sugarcane health plant is it is characterised in that be placed in sugarcane health by sugarcane health kind stem or seedling
In plant cultivating device, and described healthy plant breeding apparatus are placed in fly net seedling raising greenhouse, plant are carried out with no soil and plants
Training, timing spray nutritious liquor, regular spraying pesticide mixture, cultivating the kind stem obtaining can be used as the confession breeding healthy tissue-cultured Seedling
Body.
2. the method for claim 1, it is characterised in that described sugarcane health kind stem or seedling, is through pcr Molecular Detection
Do not carry Caulis Sacchari sinensis kind stem or the seedling of any pathogen.
3. the method for claim 1 is it is characterised in that every liter of described nutritional solution includes potassium nitrate 900~1000mg, nitre
Sour ammonium calcium 750~850mg, magnesium sulfate 350~400mg, potassium dihydrogen phosphate 170~200mg, ethylenediaminetetraacetic acid 35~40mg,
Ferrous sulfate 53~56mg, boric acid 8~8.5mg, manganese sulfate 20~25mg, zinc sulfate 1.5~1.8mg, copper sulfate 0.03~
0.06mg, sodium molybdate 0.01~0.02mg.
4. the method for claim 1 is it is characterised in that described pesticide combination includes insecticide pesticide and sterilization pesticide,
Described insecticide pesticide includes pesticide a, pesticide b and pesticide c, and described sterilization pesticide includes pesticide d and pesticide e, and its concentration is:
The gamma cyhalothrin aqueous emulsion that described pesticide a is 5% for mass concentration, concentration is 20~30ml/l;
The Rynaxypyr suspending agent that described pesticide b is 20% for mass concentration, concentration is 1~2ml/l;
Described pesticide c is 25% Diacloden water dispersible granules for mass concentration, and concentration is 1~2 gram/l;
The benzene first propiconazole suspending agent that described pesticide d is 30% for mass concentration, concentration is 0.4~1.0ml/l;
The S-Ethyl ethylthio sulfonate cream that described pesticide e is 80% for mass concentration, concentration is 0.12~0.20ml/l.
5. the method for claim 1 is it is characterised in that described sugarcane health plant cultivating device includes cultivating bucket, training
Educate bucket two side spud pile perpendicular to the ground, parallel to ground and the transverse support bar on spud pile and feed liquid conveying
Device, is provided with described cultivation bucket bucket and determines root groove, described determine root groove and is supported in cultivation bottom of the barrel by carrier;Described medicinal liquid conveying
Device includes located at the shower nozzle cultivating bucket top, time switch, air compressor machine and storage tank, and described shower nozzle passes through feeder sleeve with storage tank even
Connect.
6. it is characterised in that described cultivation bucket is Plastic Drum, it pushes up as the bucket top with crimping method as claimed in claim 4,
Bucket top internal diameter 45cm, drum head internal diameter 35cm, the high 55cm of bucket, are provided with 2 osculums at drum head 10cm.
7. it is characterised in that spray nutritious liquor is supplied with mist state form, the mode of spraying is method as claimed in claim 4: Seedling
Before phase Caulis Sacchari sinensis root system cultivates drum head, spray once within every 10 minutes, 5 seconds every time;Caulis Sacchari sinensis root system reach cultivation drum head after, daytime 7:00-
19:00, sprays once, 10 seconds every time for every 30 minutes;Evening 19:00 to the next morning 7:00, spray in every 1 hour once, every time 5
Second.
8. method as claimed in claim 4 is it is characterised in that spraying pesticide mixture sprayed once for every 30 days, and every time 10
Second.
9. method as claimed in claim 4 it is characterised in that described fly net seedling raising greenhouse insect protected reticular density be 120 mesh,
Canopy is high 5~6 meters.
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Cited By (3)
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CN107853119A (en) * | 2017-12-13 | 2018-03-30 | 佛山市高明区生产力促进中心 | A kind of method efficiently to grow cane |
CN109220748A (en) * | 2018-11-02 | 2019-01-18 | 广西壮族自治区农业科学院 | A kind of sugarcane ciltivating process |
CN114051882A (en) * | 2021-10-22 | 2022-02-18 | 湖北省农业科学院果树茶叶研究所 | Production method of kiwi fruit seedlings without canker pathogenic bacteria |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107853119A (en) * | 2017-12-13 | 2018-03-30 | 佛山市高明区生产力促进中心 | A kind of method efficiently to grow cane |
CN109220748A (en) * | 2018-11-02 | 2019-01-18 | 广西壮族自治区农业科学院 | A kind of sugarcane ciltivating process |
CN109220748B (en) * | 2018-11-02 | 2023-12-19 | 广西壮族自治区农业科学院 | Sugarcane water planting method |
CN114051882A (en) * | 2021-10-22 | 2022-02-18 | 湖北省农业科学院果树茶叶研究所 | Production method of kiwi fruit seedlings without canker pathogenic bacteria |
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