CN106342434A - Method for utilizing earthworm fungicide for improving soil nitrogen nutrients to achieve growth promotion of fast growing forest - Google Patents

Method for utilizing earthworm fungicide for improving soil nitrogen nutrients to achieve growth promotion of fast growing forest Download PDF

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CN106342434A
CN106342434A CN201610704756.3A CN201610704756A CN106342434A CN 106342434 A CN106342434 A CN 106342434A CN 201610704756 A CN201610704756 A CN 201610704756A CN 106342434 A CN106342434 A CN 106342434A
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nitrogen
soil
lumbricuss
microbial inoculum
content
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CN106342434B (en
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李永涛
张玉龙
程雄
张海春
陈晓阳
徐会娟
王利英
蔡燕飞
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South China Agricultural University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01BSOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
    • A01B79/00Methods for working soil
    • A01B79/02Methods for working soil combined with other agricultural processing, e.g. fertilising, planting

Abstract

The invention discloses a method for utilizing earthworm fungicide for improving soil nitrogen nutrients to achieve growth promotion of fast growing forest, and belongs to the technical field of forestry ecology. The method has the advantages that the effect is good, wherein the content of total nitrogen and effective nitrogen in soil is remarkably increased within one year; the content of nitrogen is greatly improved, wherein accumulation of total nitrogen and effective nitrogen in soil has the remarkable synergistic interaction effect; multiple purposes are achieved at the same time, wherein due to application of pig manure, biogas slurry combined with earthworms and fungicide, the content of effective nitrogen in soil can be rapidly increased, the nitrogen fertility stability can be rapidly improved, growth of fast growing forest can be promoted, the branch diameter, the plant height and the crown breadth are improved by 1.7 times, 1.3 times and 5.1 times compared with CK, and livestock excrement pollution is digested and saved. A research shows that due to application of earthworms and fungicide, the decomposition rate of pig manure in soil can be increased, so that the soil nitrogen content is increased; some nitrogen in air which cannot be utilized by plants can be converted into available nitrogen elements; earthworms and fungicide contain carbon and nitrogen nutrients, and due to the combined application, the content of nitrogen in soil can be increased.

Description

A kind of method that utilization Lumbricuss microbial inoculum improves soil nitrogen nutrient growth-promoting fast-growing forest
Technical field
The invention belongs to Forestry Ecological technical field, improve soil nitrogen nutrient rush particularly to a kind of using Lumbricuss microbial inoculum The method of raw fast-growing forest.
Background technology
The development of aquaculture in recent years brings substantial amounts of feces of livestock and poultry, the animal dung of China's large-scale cultivation industry in 2015 Just up to 32.6 hundred million tons of yield.The dietary element such as substantial amounts of n, p is contained, as Agricultural organic fertilizer in feces of livestock and poultry Both can fertilizing soil, for plant growing provide needed for nutrient, improve crop yield, and can dispose a large amount of feces of livestock and poultry, solution ring Border pollution problem.
Nitrogen is one of indispensable element of plant growing, is to constitute one of of paramount importance element of life entity, is reflection soil Loamy texture amount, the important indicator of the farmland ecosystem productivity.China's soil supply nitrogen is less, leads to agricultural land soil generally to lack Nitrogen.Applied nitrogen is the important channel improving soil nitrogen nutrient, but China's utilization rate of nitrogen fertilizer ratio is relatively low, only 30~40%. Apply for a long time the holding capabilities of a large amount of inorganic nitrogeneous fertilizers easily absorbability beyond crop and soil, lead to soil nitrogen to drench Lose, and after organic fertilizer applies, wherein Nitrogen releasing is slow, easily accumulates in soil.Therefore, reasonably utilize organic fertilizer, Accelerate fertilizer Nitrogen releasing, release nitrogen nutrition of simultaneously quickly dissolving, minimizing nutrient loss, this is to increasing soil fertility, real Existing environmental conservation and agricultural sustainable development etc. are significant.
Cn 103058720 a has invented a kind of method that protease promotes straw degradative to strengthen soil fertility.In temperature Under the conditions of 20~29 DEG C of degree, it is 0.1~8cm segment that straw is cut smashing, liquid of protease is sprayed on crushing straw and is earthed Earth.Because agricultural crop straw contains macronutrient and the trace element needed for crop, also field can be inhaled as fertilizer The most of Nutrient eiement return received is to soil.This invention addresses the nitrogen content that the straw-returning initial stage occurs and decline problem, can To improve Soil Nitrogen Content, soil available nitrogen content improves more than 45%, can improve soil physical property, strengthens soil fertile Power, improves crop growth environment, turns waste into wealth.
Cn 105218266 a has invented a kind of ardealite soil conditioner improving Soil Nitrogen Content, described modifying agent It is prepared from the following raw materials in parts by weight: plant ash 23~27, discard green grass or young crops lemon skin 4~5, furfural dregs 15~17, carbamide 12~15, sulphuric acid Nickel 1~2, calcium lactate 3~5, ardealite 90~110, red mud 18~22, carbon method filter mud 9~11, en microbial inoculum 3~4, appropriate amount of water.System Multiple acid ingredients in ardealite during standby can be complementary with red mud strong basicity, adjusts the ph of improved soil, blue or green lemon micromicro with Increase the content of agron.The present invention applies during Rice Cropping, and the alkaline hydrolysis nitrogen content of paddy soil can be made to improve 20.6%, soil quality can be strengthened, delay the degeneration of soil, improve soil and can be increase soil fertility using nitrogen content, improvement Soil nutrient elements are constituted.
Cn 101519321 a discloses North China's moisture soil wheat and corn feces of livestock and poultry Organicchemical fertilizers method. With the ratio according to 1200/7.25 (weight ratio) for the feces of livestock and poultry fertilizer and carbamide being formed after feces of livestock and poultry pile fermentation Apply, it is long that the method both can give full play to feces of livestock and poultry fertilizer fertilizer efficiency, improved soil structure and fertility, speed can be played again Effect inorganic nitrogeneous fertilizer can be rapidly provided the biological growth wilderness demand fertile to n, make two kinds of fertilizer give full play to respective feature, increase Product quality, improves crop yield, improved soil, promotes the development in rural area.
Cn 104529571 a has invented the fertilizer processing technology of a kind of microorganism fungus kind and Lumbricuss Duplex treatment, passes through Plant fiber, poultry manure, microorganism fungus kind and Lumbricuss are made, the fertilizer of production can chesson, significantly improve Soil fertility.
At present, technical research both domestic and external focuses mostly on and prepares (Lumbricuss, microbial inoculum, enzyme etc.), fertilizer and chemical fertilizer in fertilizer Join and the method such as apply and improve nitrogen in farmland soil content, strengthen soil fertility, and be directed to barren arid hillside field, soil in-situ is joined and applied natural pond Liquid, Lumbricuss, microbial inoculum, accelerate the method that pig manure nitrogen (organic nitrogen) converts almost not have.And prior art generally existing slowly effect, The shortcomings of preparation process is loaded down with trivial details, formula is complicated.
Content of the invention
In order to overcome shortcoming and the deficiency of prior art, it is an object of the invention to provide a kind of improved using Lumbricuss microbial inoculum The method of soil nitrogen nutrient growth-promoting fast-growing forest.The method is by two kinds of Lumbricuss, composite functional bacterial agent, collaborative pig manure, natural pond The original position of liquid is applied, and both improved arid hillside field soil available nitrogen content rapidly, and is quickly absorbed by fast-growing forest, meets growth need Ask, reduce Nitrogen Leaching, be finally reached increase soil fertility, growth-promoting fast-growing forest, pig manure garbage of dissolving, reduce pollution mesh 's.
The purpose of the present invention is achieved through the following technical solutions:
A kind of method that utilization Lumbricuss microbial inoculum improves soil nitrogen nutrient growth-promoting fast-growing forest, specifically utilizes Lumbricuss, bacterium Pig manure is worked in coordination with agent, biogas slurry increases arid hillside field soil nitrogen nutrient, and release nitrogen nutrient is rapidly absorbed, thus promoting hillside fields fast-growing Woods growth, a kind of method of minimizing pollution of area source;(mytilaria arranged always by Anthocephalus chinensis (neolamarckia cadamba), rice Laosensis) fast-growing woods ground is located in pig farm, about 40 degree of the gradient, and every plant of forest spacing 5~6m, in the upper half way up the mountain of every plant of forest Dig annular ditch, for the applying of fertilizer and microbial inoculum, Lumbricuss, set up and be used for irrigating biogas slurry from gravity hillside fields drip irrigation system, point season Section applies pig manure, biogas slurry, composite bacteria agent capable, mixing Lumbricuss, comprises the steps:
(1) fall and winter applying pig manure 2.5~20kg//time, winter sowing amount is the half in autumn, applying mode It is to dig a depth in 20~30cm annular ditch in the upward slope direction of tree, pig manure is equably sprinkled into wherein, earthing is mixed thoroughly, landfill;
(2) biogas slurry will monthly be applied, sowing amount is 2.5~20l//time, the dry season frequency is higher than rainy season;
(3) applied composite bacteria agent capable once every three months, applied amount is 0.25~1l//time, and each amount of application reaches and has Effect viable count≤1011Cfu, fertilization mode is that pouring applies;
(4) every three months apply mixing Lumbricuss once, each sowing amount be 80~320// time.Nitrogen in earthworm excrement Content is about 1.2~1.7 times of soil, on the one hand can improve the available nitrogen content in soil;One side earthworm movement The speed of Nitrogen Cycling in soil and muck can be improved.
Pig manure described in step (1) is the pig manure slightly becoming thoroughly decomposed;In the pig manure becoming thoroughly decomposed, itself contains substantial amounts of nitrogen nutrition Element.
The fermentation raw material of the biogas slurry described in step (2) is Pig dung and urine and punching circle waste water, adopts from gravitational drip irrigation system, Pvc pipeline and dropper technology are irrigated, and accomplish breeding wastewater zero-emission;
Composite bacteria agent capable described in step (3) includes bacillus subtilises (bacillus subtilis) hl-1, wax-like bud In spore bacillus (bacillus cereus) nc-1 and bacillus cereuss (bacillus cereus) b19 at least 2 kinds.Above-mentioned Bacterial strain heavy metal has good toleration, will not because in pig manure heavy metal accumulate in soil and inactivate or dead, and can With by n in air2It is reduced to ammonia, increase Soil Nitrogen Content.
Described bacillus subtilises (bacillus subtilis) hl-1 patent " 201110282657.8, withered Careless bacillus cereuss hl-1 and its in the application in terms of soil phosphorus decomposing " disclosed in, its preservation information is: protects within 24th in August in 2011 It is hidden in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number cgmcc no.5175.
Described Bacillus cercuses (bacillus cereus) nc-1 is in " 201210573809.4, one plant of patent Disclosed in the Bacillus cercuses of activating soil nutrient and its application ", this bacterial strain is preserved in Chinese micro- life on May 21st, 2012 Thing culture presevation administration committee common micro-organisms center (cgmcc), deposit number cgmcc no.6127;
Described bacillus cereuss (bacillus cereus) b19 has tolerance and heavy metal-passivated function, its guarantor Tibetan information is: was preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on 04 27th, 2016, protects Hide address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, deposit number cgmcc no.12405.
Each amount of application described in step (3) reaches living bacteria count and is preferably 1010~1011cfu;
Mixing Lumbricuss described in step (4) include two kinds, be top layer kind Eisenia foetida (eisenia foetida) and The grand Pheretimatschiliensis of internal layer kind (amynthas robustus), the described grand Pheretimatschiliensis of internal layer kind and top layer kind Eisenia foetida Mixing quantitative proportion be preferably 1:3~1:7.
The present invention, with respect to prior art, has such advantages as and effect:
(1) effect is good.Show in this test, total nitrogen of soil and effective nitrogenous component significantly raised in 1 year.Fast-growing The height of crop, hat width are significantly higher than matched group.
(2) nitrogen content lifting amplitude is big.After applying fertilizer 1 year in the present invention, apply pig manure, biogas slurry, Lumbricuss, microbial inoculum simultaneously Total nitrogen content of soil relatively not fertilizer treatment (ck) improve 77.2%, relatively apply pig manure and the treatment group of biogas slurry improve 57.3%, from the nitrogen component of accumulation, in soil, more than 90% nitrogen is that existed with organic compound.This method is applied Alkaline hydrolysis available nitrogen improves 63.3% relative to ck afterwards, and acidolysis total nitrogen content improves 55.2% with respect to ck, illustrates that a term applies Plus pig manure, biogas slurry, microbial inoculum and the full nitrogen of Soil of Earthworms, the accumulation of available nitrogen have significant synergistic function.
(3) achieve many things at one stroke.Pig manure, biogas slurry joint Lumbricuss, the administration of microbial inoculum not only can quickly improve soil available nitrogen and contain Amount and the stability of Soil fertility, and the growth of fast-growing wood can be promoted, base footpath, plant height, hat width carry respectively with respect to ck High 1.7 times, 1.3 times, 5.1 times, Animal manure loss of dissolving.
(4) present invention adopts the method that pig manure worked in coordination with by Lumbricuss, microbial inoculum, biogas slurry improves Soil Nitrogen Content first.Research table Bright, 1. Lumbricuss, the applying of microbial inoculum can improve the decomposition rate of pig manure in soil Soil Nitrogen Content is raised;2. in the air Some can not by plant using nitrogen be converted into available nitrogen;3. Lumbricuss, microbial inoculum itself contain carbon, nitrogen nutrient, its conjunction Apply and can improve Soil Nitrogen Content.
Brief description
Fig. 1 is the changes of contents figure of different disposal total soil nitrogen (tn).
Fig. 2 is the changes of contents figure of different disposal soil alkali-hydrolyzable nitrogen (an)
Fig. 3 is the changes of contents figure of different disposal acid digestion total nitrogen (hn).
Specific embodiment
With reference to embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention do not limit In this.
With reference to specific test, the present invention is further illustrated.
Experimental field it is located at Yunfu City Xinxing County east Cheng Zhenluo old Hua Nongwenshi pig farm.Testing site soil is crimson Earth.Testing site soil 0~20cm topsoil nutrient base value: organic carbon content 10.44g kg-1, total nitrogen content 0.88g·kg-1, available phosphorus contents 21.95g kg-1, full potassium content 1.71g kg-1, quick-acting potassium content 33.67g kg-1.
Embodiment 1
A kind of method that utilization Lumbricuss microbial inoculum improves soil nitrogen nutrient growth-promoting fast-growing forest, using pig manure, biogas slurry, earthworm The method that earthworm, microbial inoculum improve Soil Nitrogen Content, in Anthocephalus chinensis fast-growing forest land around every plant of forest 1m2In the range of, soil 0~ In the depth bounds of 20cm, point season applies pig manure, biogas slurry, composite bacteria agent capable and mixing Lumbricuss, comprises the steps of: 2013 August and in November, 2013 apply pig manure (becoming thoroughly decomposed) 10kg//time, 5kg//time respectively;(2013 from the beginning of test arrangement August) arrange 1 year (in August, 2014) to test, 4~September applies biogas slurry amount for rainy season and is 15l//time, totally 4 times, 10~March Apply biogas slurry amount for dry season and be 15l//time, totally 10 times;Apply composite bacteria agent capable bacillus subtilises every three months (bacillus subtilis) hl-1 and Bacillus cercuses (bacillus cereus) nc-1 mix bacterium agent 0.5l//time, Viable count reaches 1010Cfu, the quantitative proportions of two kinds of mixing Lumbricuss are 1:5, apply the grand Pheretimatschiliensis of 30 internal layer kinds and 150 Top layer kind Eisenia foetida// time.
Embodiment 2
A kind of method that utilization Lumbricuss microbial inoculum improves soil nitrogen nutrient growth-promoting fast-growing forest, is improved using Lumbricuss, microbial inoculum The method of Soil Nitrogen Content, arranges fast-growing forest land around every plant of forest 1m always in rice2In the range of, the depth of soil 0~20cm In the range of, point season applies pig manure, biogas slurry, composite bacteria agent capable and mixing Lumbricuss, comprises the steps of: in Augusts, 2013 and 2013 November in year applies pig manure (becoming thoroughly decomposed) 20kg//time, 10kg//time respectively;From the beginning of test arrangement, (in August, 2013) is to examination Perching is put 1 year (in August, 2014), and 4~September applies biogas slurry amount for rainy season and is 10l//time, totally 4 times, and 10~March applied for dry season Plus biogas slurry amount is 20l//time, totally 10 times, applied composite bacteria agent capable bacillus subtilises (bacillus every three months Subtilis) hl-1 and Bacillus cercuses (bacillus cereus) nc-1 mix bacterium agent 1l//time, viable count reaches 1011Cfu, the quantitative proportion of two kinds of mixing Lumbricuss is 1:7, applies 40 grand Pheretimatschiliensis of internal layer kind and 280 top layer kind compatriots Love victory earthworm// time.
Embodiment 3
A kind of method that utilization Lumbricuss microbial inoculum improves soil nitrogen nutrient growth-promoting fast-growing forest, is improved using Lumbricuss, microbial inoculum The method of Soil Nitrogen Content, in Anthocephalus chinensis fast-growing forest land around every plant of forest 1m2In the range of, the depth of soil 0~20cm In the range of, point season applies pig manure, biogas slurry, composite bacteria agent capable and mixing Lumbricuss, comprises the steps of: in Augusts, 2013 and 2013 November in year applies pig manure (becoming thoroughly decomposed) 5kg//time, 2.5kg//time respectively;From the beginning of test arrangement, (in August, 2013) is to examination Perching is put 1 year (in August, 2014), and 4~September applies biogas slurry amount for rainy season and is 2.5l//time, totally 4 times, and 10~March is dry season Apply biogas slurry amount and be 5l//time, totally 10 times, apply composite bacteria agent capable bacillus subtilises (bacillus every three months Subtilis) hl-1, Bacillus cercuses (bacillus cereus) nc-1 and bacillus cereuss (bacillus Cereus) b19 mix bacterium agent 0.25l//time, viable count reaches 1010Cfu, the quantitative proportion of two kinds of mixing Lumbricuss is 1:3, applies Plus 20 grand Pheretimatschiliensis of internal layer kind and 60 internal layer kind Eisenia foetida// time.
Effect example 1
From the point of view of, apply pig manure, biogas slurry, mixing Lumbricuss, the process of composite bacteria agent capable taking the implementation result of embodiment 1 as a example simultaneously T (e+b), applies the process (tb) of pig manure, biogas slurry and composite bacteria agent capable, applies the process (te) of pig manure, biogas slurry and mixing Lumbricuss, with Do not apply fertilizer and only apply a process (ck) for irrigation water, apply the process (t or pm+s) of pig manure and biogas slurry as comparison simultaneously.In 2013 Year August, November and 2014 2 months, May, before August fertilising collection 0~20cm topsoil pedotheque, measure soil after treatment Total nitrogen content (tn), alkali-hydrolyzable nitrogen (an) content, nitrate nitrogen (no3 -- n) content, ammonium nitrogen (nh4+- n) content and acidolysis total nitrogen (hn) (organic nitrogen) content.
Table 1 soil tn (g.kg-1)、an(mg.kg-1)、no3 --n(mg.kg-1)、nh4 +-n(mg.kg-1)、hn(mg.kg-1) Content
As shown in table 1 and Fig. 1, total nitrogen content of soil assumes the trend gradually rising to result on the whole, before test arrangement, cloth Put three months, respectively process total soil nitrogen (tn) content and significant difference, 6 months each process total soil nitrogen of test arrangement do not occur Significant difference in content table, and the soil tn content of t (e+b) is significantly raised with respect to other process, is significantly higher than ck and t Process, ck process relatively improves 57.1%, t process relatively improves 32.5%, te and tb processing difference relatively is not notable;Examination Perching is put 9 months, and the soil tn content of t (e+b) is significantly higher than other process (p < 0.05), and ck process relatively improves 100%, t process relatively improves 58%, and 12 months each soil tn contents that process of test arrangement still show significant difference (p < 0.05), the soil tn content of t (e+b) improves 77.2% relative to ck process, and t process relatively improves 57.3% soil. Result shows, the strong and weak order that long-term fertilization improves total nitrogen content of soil ability is: t (e+b) > te > tb > t > ck.To sum up Described, the process of the present invention can make total nitrogen content of soil assume the trend being gradually increased in 1 year, improves inorganic nitrogen in soil Content.
As shown in table 1 and Fig. 2, before test arrangement, each soil alkaline hydrolysis nitrogen content that processes does not show significant difference to result (p < 0.05), 3 months each soil alkali-hydrolyzable nitrogen contents that process of test arrangement show significant difference (p < 0.05), and at fertilising Reason all shows significant difference higher than the alkaline hydrolysis nitrogen content of not fertilizer treatment ck, afterwards each batch soil alkaline hydrolysis nitrogen content, and Test arrangement apply within 1 year simultaneously pig manure, biogas slurry, Lumbricuss, microbial inoculum process t (e+b) be significantly higher than other process.Soil ammonium With nitrate nitrogen content compared with before test arrangement, entirety rises the effect that do not increase significantly to state nitrogen.Soil alkaline hydrolysis nitrogen content is described Increase be not due to, caused by the increase of content of inorganic nitrogen in soil, lead to it because of the increase of SOIL ORGANIC NITROGEN content The increase of content.
Result, as shown in table 1 and Fig. 3, each before test arrangement process acid digestion total nitrogen content not show significance poor Different (p < 0.05), three months each acid digestion total nitrogen contents that process of test arrangement show significant difference (p < 0.05), t (e + b) acid digestion total nitrogen content process significantly raised with respect to other, each batch respectively processes acid digestion total nitrogen content afterwards Significant difference (p < 0.05) all occurs, and the acid digestion total nitrogen in test arrangement 1 year t (e+b) improves with respect to ck 50.9%, improve 23.3% with respect to t, each acid digestion total nitrogen content sequence that processes is t (e+b) > te > tb > t > ck.In sum, the process of the present invention can improve the content of SOIL ORGANIC NITROGEN.
The analysis of result: pig manure, biogas slurry are joined and applied process and make SOIL ORGANIC NITROGEN and content of inorganic nitrogen in all increased.Lumbricuss It is applied in pig manure agricultural soil and can significantly improve Soil Nitrogen Content, and microbial inoculum is not bright to the raising effect of soil nitrogen Aobvious, but when with Lumbricuss, microbial inoculum be applied to simultaneously pig manure, in biogas slurry agricultural soil to the lifting effect of organic and inorganic nitrogen content For obvious, reason is probably pig manure, biogas slurry, join apply Lumbricuss, the applying of microbial inoculum can significantly improve soil carbon content, the applying of Lumbricuss Plus accelerate the conversion of soil carbon, the organic characteristic of soil can be improved, thus increasing the active organic nitrogen pool of soil, separately On the one hand it is possibly due to contain nitrogen-fixing microorganism in bacteria agent, can be by n in air2It is reduced to ammonia, therefore, it is possible to increase Nitrogen content in soil.
Yellow fine strain of millet wood growth indexes, are shown in Table 2.
The yellow fine strain of millet wood growth indexes of table 2
Result as shown in table 2, apply on the whole simultaneously pig manure, biogas slurry, mixing Lumbricuss, composite bacteria agent capable process can be notable Promote the growth of yellow fine strain of millet wood.Test arrangement three months, this application process begins to play a role, and base footpath, plant height, hat width are respectively 3.4 times, 3.7 times, 6.9 times of ck process.After 1 year, in t (e+b) process, the base footpath of yellow fine strain of millet wood, plant height, hat width are arrangement respectively 2.7 times, 2.3 times, 6.1 times of ck process.
Above example all can improve Soil Nitrogen Content, the digestion rate of pig manure, soil fertility, and three sample results Similar.
In sum, the present invention is integrated with pig manure fermentation, the irrigation and drainage system of biogas slurry, the exploitation of Lumbricuss, the money of bacterial manure The technology such as the cultivation of sourceization utilization and Anthocephalus chinensis, define " pig manure biogas slurry Lumbricuss bacterial manure crop " ecological use mould Formula, decreases pig manure to the pollution of environment it is achieved that recycling, can be for setting up good fertilizing system and soil changes The data supporting of good offer science.
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention are not subject to above-described embodiment Limit, other any spirit without departing from the present invention and the change made under principle, modification, replacement, combine, simplify, All should be equivalent substitute mode, be included within protection scope of the present invention.

Claims (6)

1. a kind of utilization Lumbricuss microbial inoculum improve soil nitrogen nutrient growth-promoting fast-growing forest method it is characterised in that: specifically profit Work in coordination with pig manure, biogas slurry increase arid hillside field soil nitrogen nutrient with Lumbricuss, microbial inoculum, release nitrogen nutrient is rapidly absorbed, thus promoting Enter hillside fields fast-growing woods growth, a kind of method of minimizing pollution of area source;Dig annular ditch in the upper half way up the mountain of every plant of forest, for fertilizer With the applying of microbial inoculum, Lumbricuss, set up and be used for irrigating biogas slurry from gravity hillside fields drip irrigation system, point season applies pig manure, biogas slurry, is combined Microbial inoculum, mixing Lumbricuss, comprise the steps:
(1) fall and winter applies pig manure 2.5~20kg//time, and winter sowing amount is the half in autumn, applying mode be A depth is dug in 20~30cm annular ditch in the upward slope direction of tree, pig manure is equably sprinkled into wherein, earthing is mixed thoroughly, landfill;
(2) biogas slurry will monthly be applied, sowing amount is 2.5~20l//time, the dry season frequency is higher than rainy season;
(3) applied composite bacteria agent capable once every three months, applied amount is 0.25~1l//time, and each amount of application reaches effectively alive Bacterium number≤1011Cfu, fertilization mode is that pouring applies;
(4) every three months apply mixing Lumbricuss once, each sowing amount be 80~320// time.
2. the method that utilization Lumbricuss microbial inoculum according to claim 1 improves soil nitrogen nutrient growth-promoting fast-growing forest, it is special Levy and be:
Described fast-growing forest is Anthocephalus chinensis (neolamarckia cadamba), rice arranges (mytilaria laosensis) always.
3. the method that utilization Lumbricuss microbial inoculum according to claim 1 improves soil nitrogen nutrient growth-promoting fast-growing forest, it is special Levy and be:
Composite bacteria agent capable described in step (3) includes bacillus subtilises (bacillus subtilis) hl-1, wax-like spore bar In bacterium (bacillus cereus) nc-1 and bacillus cereuss (bacillus cereus) b19 at least 2 kinds.
4. the method that utilization Lumbricuss microbial inoculum according to claim 1 improves soil nitrogen nutrient growth-promoting fast-growing forest, it is special Levy and be:
It is 10 that each amount of application described in step (3) reaches living bacteria count10~1011cfu.
5. the method that utilization Lumbricuss microbial inoculum according to claim 1 improves soil nitrogen nutrient growth-promoting fast-growing forest, it is special Levy and be:
Mixing Lumbricuss described in step (4) include two kinds, are top layer kind Eisenia foetida (eisenia foetida) and internal layer Plant grand Pheretimatschiliensis (amynthas robustus).
6. the method that utilization Lumbricuss microbial inoculum according to claim 5 improves soil nitrogen nutrient growth-promoting fast-growing forest, it is special Levy and be: the described grand Pheretimatschiliensis of internal layer kind are 1:3~1:7 with the mixing quantitative proportion of top layer kind Eisenia foetida.
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CN110747151A (en) * 2019-12-06 2020-02-04 华南农业大学 Culture medium composition for promoting natural growth and transformation of bacillus subtilis and transformation method thereof
CN113973667A (en) * 2021-10-21 2022-01-28 江南大学 Nano biological regulation and control method for improving growth, yield and quality of corn

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