CN106324121B - 一种虫草类药材样品中两种微量核苷的检测方法 - Google Patents
一种虫草类药材样品中两种微量核苷的检测方法 Download PDFInfo
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- CN106324121B CN106324121B CN201610648359.9A CN201610648359A CN106324121B CN 106324121 B CN106324121 B CN 106324121B CN 201610648359 A CN201610648359 A CN 201610648359A CN 106324121 B CN106324121 B CN 106324121B
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- OLXZPDWKRNYJJZ-UHFFFAOYSA-N 5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-ol Chemical compound C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(CO)O1 OLXZPDWKRNYJJZ-UHFFFAOYSA-N 0.000 claims abstract description 44
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- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 9
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- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims description 6
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- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 3
- 235000019253 formic acid Nutrition 0.000 claims description 3
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- SVWLIIFHXFGESG-UHFFFAOYSA-N formic acid;methanol Chemical compound OC.OC=O SVWLIIFHXFGESG-UHFFFAOYSA-N 0.000 abstract description 2
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N30/08—Preparation using an enricher
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/062—Preparation extracting sample from raw material
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
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CN109115931A (zh) * | 2017-06-26 | 2019-01-01 | 苏州旭辉检测有限公司 | 一种血液样本中虫草素的检测方法 |
CN107796895B (zh) * | 2017-09-21 | 2020-12-18 | 东莞市东阳光冬虫夏草研发有限公司 | 鲜冬虫夏草在线提取高效液相特征图谱的构建方法及应用 |
CN111610268B (zh) * | 2020-06-02 | 2021-09-14 | 东莞市东阳光冬虫夏草研发有限公司 | 分离待测样品中甘露醇、腺苷和麦角甾醇的方法及应用 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104330512A (zh) * | 2014-10-28 | 2015-02-04 | 中国农业科学院农业质量标准与检测技术研究所 | 基于在线SPE的快速测定尿液中轭合态β-受体激动剂的方法 |
CN105467019A (zh) * | 2014-08-18 | 2016-04-06 | 中粮营养健康研究院有限公司 | 维生素a、e的测定方法和相应的在线固相萃取分析系统 |
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2016
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105467019A (zh) * | 2014-08-18 | 2016-04-06 | 中粮营养健康研究院有限公司 | 维生素a、e的测定方法和相应的在线固相萃取分析系统 |
CN104330512A (zh) * | 2014-10-28 | 2015-02-04 | 中国农业科学院农业质量标准与检测技术研究所 | 基于在线SPE的快速测定尿液中轭合态β-受体激动剂的方法 |
Non-Patent Citations (4)
Title |
---|
九州虫草中核苷类成分的HPLC法测定;罗珊珊等;《中草药》;20051031;第36卷(第10期);第1-2部分 |
冬虫夏草不同部位核苷类成分比较分析;钱正明等;《世界科学技术-中医药现代化》;20141231;第16卷(第11期);第2390-2394页 |
固相萃取-高效液相色谱法测定蛹虫草中虫草素的含量;孔英戈等;《南华大学学报》;20070630;第21卷(第2期);第2.2、3.3部分 |
高效液相色谱定量分析比较4种虫草药材的核苷类成分;钱正明等;《中国中药杂志》;20160731;第41卷(第13期);第2493-2499页 |
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Denomination of invention: Two micro-nucleosides testing method for medicine samples of insect and herbage class Effective date of registration: 20190903 Granted publication date: 20190301 Pledgee: Yichang Branch of Bohai Bank Co., Ltd.|Three Gorges Branch of Bank of China Co., Ltd.|Nanhu Branch of Hubei Bank Co., Ltd.|Yichang Branch of Hankou Bank Co., Ltd. Pledgor: Guangdong Dongguang Sunshine Pharmaceutical Co., Ltd.|Yichang Shancheng Shuidu Cordyceps sinensis Co., Ltd. Registration number: Y2019420000010 |
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Denomination of invention: A method for the determination of two trace nucleosides in Cordyceps Effective date of registration: 20210630 Granted publication date: 20190301 Pledgee: Bank of Bohai Limited by Share Ltd. Yichang branch|Bank of China Limited by Share Ltd. Three Gorges Branch|Bank of Hubei Limited by Share Ltd. Yichang Nanhu sub branch|Bank of Hankou Limited by Share Ltd. Yichang branch Pledgor: YICHANG SHANCHENGSHUIDU CORDYCEPS Co.,Ltd. Registration number: Y2021420000062 |