CN106305794A - Method for preparing residue-free plant insecticide by using undaria pinnatifida - Google Patents
Method for preparing residue-free plant insecticide by using undaria pinnatifida Download PDFInfo
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- CN106305794A CN106305794A CN201610721860.3A CN201610721860A CN106305794A CN 106305794 A CN106305794 A CN 106305794A CN 201610721860 A CN201610721860 A CN 201610721860A CN 106305794 A CN106305794 A CN 106305794A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/03—Algae
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Abstract
The invention discloses a method for preparing a residue-free plant insecticide by using undaria pinnatifida and belongs to the technical field of insecticide preparation. Undaria pinnatifida pieces, ethyl alcohol and tea polyphenol are smashed into slurry, then suction filtration is performed, the collected filtrate is respectively subjected to rotary evaporation, pH is regulated, a prepared acidized extracting solution is stirred with diethyl ether, rotary evaporation is performed after extraction treatment, a phytohormone is obtained, then a collected filter cake is washed and dried and then is stirred and heated with a cellulose water solution, rotary evaporation treatment is conducted on the filtrate after filtration, the hydrolyzed undaria pinnatifida gel liquid prepared later and a sodium hydroxide solution are stirred and subjected to ultrasonic oscillation, and finally ultrasonic dispersing treatment is conducted on the obtained alkalized undaria pinnatifida gel liquid, the prepared phytohormone, celastrus angulatus and the like to obtain the residue-free plant insecticide. The prepared residue-free plant insecticide is quick in insecticide effect release and good in deinsectization effect, is easily decomposed after being sprayed on crops and is an ideal plant insecticide, residual toxicity is avoided, and no harm is caused to the environment and health of human and livestock.
Description
Technical field
The invention discloses a kind of method that Thallus Laminariae prepares noresidue vegetable insecticide, belong to insecticide technology of preparing neck
Territory.
Background technology
Insecticide, is mainly used in preventing and treating agricultural pests and the medicine of urban health insect.Use history is long-range, consumption big,
Wide in variety.At twentieth century, developing rapidly of agricultural, insecticide makes farm output rise higher.But, nearly all insecticide all can
Seriously changing ecosystem, major part is harmful, and other meeting is concentrated in food chain.We must send out in agricultural
Exhibition and environment and health obtain balance.
Use botanical pesticide prevention and elimination of disease and pests, in China's history of existing 1500, Compendium of Material Medica has just had use
The record of medical herbs parasite killing.Study according to investigations, have 200 various plants can be processed into natural plant medicament, be used for preventing and treating agricultural fruit and vegerable
Crop disease and insect.How the world today alleviates chemical pesticide is polluted, it is ensured that food safety, produces Organic food, nuisanceless green
Food, the most increasingly gets more and more people's extensive concerning.It can be said that appliable plant pesticide, in terms of ecological protection, it is special to have
The most replaceable advantage.
Along with the living standard of people improves constantly, while health problem is paid close attention to by people, also pursuing protoplasm original flavor
Vegetable and fruit, pursue environment protection.Therefore, the green non-pollution herbal insecticidal agent that a kind of energy is long-term, efficient, practical
Synthesis has realistic meaning.
Slowly, deinsectization effect is undesirable for the release of its drug effect of the vegetable insecticide of existing use, can be residual on plant after using
Stay trace toxin, crop growth environment and people, animal health are caused harmful effect in various degree.
Summary of the invention
The technical problem that present invention mainly solves: there is drug effect release slowly for currently used vegetable insecticide, go out
Worm effect is undesirable, after using on plant can residual minim toxin, the problem that environment and people, animal health can be worked the mischief,
Thering is provided a kind of method that Thallus Laminariae prepares noresidue vegetable insecticide, Thallus Laminariae fragment is pounded by the method with ethanol, tea polyphenols
Sucking filtration after serosity, regulates pH after the filtrate rotation collected being steamed respectively, and prepared acidizing extracting liquid is agitated with ether, extraction processes
Rear rotation is steamed, and obtains auximone, then by dried for the Cake Wash of collection and cellulase aqueous solution agitating heating, after filtering
Filtrate being made rotation steaming process, the hydrolysis Thallus Laminariae coagulant liquid prepared subsequently and sodium hydroxide solution stir sonic oscillation, finally will
The alkalization Thallus Laminariae coagulant liquid obtained carries out ultrasonic disperse process with the auximone of above-mentioned preparation, Celastrus angulatus etc.,
Obtaining a kind of noresidue vegetable insecticide, the vegetable insecticide drug effect release that the present invention prepares is fast, and good bug eliminating effect is sprayed at crop
Upper easily decomposition, it is to avoid retain residual hazard, materials safety environmental protection, will not work the mischief environment and people, animal health, is a kind of preferable
Vegetable insecticide.
In order to solve above-mentioned technical problem, the technical solution adopted in the present invention is:
(1) choose Thallus Laminariae and clean with clear water, dried and shred, being prepared into Thallus Laminariae fragment, the most by weight
Meter, weighs 45～50 parts of mass fraction 80% ethanol, 3～5 parts of tea polyphenols and 20～25 parts of Thallus Laminariae fragments respectively and is placed in tissue and smashes
In broken machine, smash process 2～3min to pieces；
(2) after having smashed to pieces, collect Thallus Laminariae fragment serosity, to its sucking filtration and collect filtrate and filter cake, filtrate is placed in 30
～rotation is evaporated to the 1/5 of original volume at 40 DEG C, collects and concentrate Thallus Laminariae extracting solution, regulate pH to 3.0 with glacial acetic acid, be prepared into
Acidizing extracting liquid, then 1:5 by volume, mix the acidizing extracting liquid of above-mentioned preparation with ether stirring, and extraction processes 3～5 times
After, merge ether phase, rotary evaporated to dryness at 30～40 DEG C, collect to obtain auximone, standby；
(3) filter cake of above-mentioned collection is washed with deionized to cleaning mixture pH to 7.0, at 65～70 DEG C, is dried 6～8h, receives
Collection dried undaria pinnatifida fiber, in mass ratio 5:1, stir mass fraction 10% cellulase aqueous solution with dried undaria pinnatifida fiber
Mixing, at 25～30 DEG C, heating in water bath 45～60min, filters subsequently and collects filtrate, rotating and be evaporated at 45～60 DEG C
The 1/5 of original volume, is prepared into hydrolysis Thallus Laminariae coagulant liquid；
(4) 1:3 in mass ratio, by the hydrolysis Thallus Laminariae coagulant liquid stirring of mass fraction 10% sodium hydroxide solution with above-mentioned preparation
Mixing, under 200～300W, sonic oscillation processes 10～15min, collects alkalization Thallus Laminariae coagulant liquid, the most by weight
Meter, weighs plant prepared by 45～50 parts of alkalization Thallus Laminariae coagulant liquids, 35～40 parts of deionized waters, 5～10 parts of steps (2) respectively
Auxin, 10～15 parts of Celastrus angulatus and 10～15 parts of phenethyl phenol polyethenoxy ethers are placed in conical flask, 200～300W
Lower ultrasonic disperse 10～15min, stands 6～8h, can be prepared into a kind of noresidue vegetable insecticide.
The application process of the present invention is: be parasite killing in the ratio dilute of insecticide and water proportion 1:800～1:1000
Medicinal liquid, is sprayed directly on parasite killing medicinal liquid on the plants such as fruit tree, crops and vegetable, and spray survival dose is with every mu 30～35kg parasite killing
Medicinal liquid.After testing, the insect pest of the noresidue vegetable insecticide energy effectively preventing plant that the present invention prepares, and materials safety
Environmental protection, after dispenser, drug effect release is fast, is easily absorbed by plants decomposition, does not retains residual hazard on plant, and after using 1～3 day, parasite killing is imitated
Fruit reach more than 92%, the vegetable insecticide of the present invention after administration 35～40 days time, still keep higher preventive effect, holding of insecticide
The effect phase is long.
The invention has the beneficial effects as follows:
(1) the noresidue vegetable insecticide feedstock capture low cost of the present invention, makes simple and convenient, to plant and crops etc. all
There is good insecticidal effect, and environmentally safe, people, animal health will not be worked the mischief, meet the requirement of green production；
(2) the noresidue vegetable insecticide of the present invention is a kind of high efficacy pesticide for plant, has the effect of broad spectrum disinfestation, is sprayed at
Easily decompose on crop, it is to avoid retaining residual hazard, and drug effect release is fast, insecticidal effect is obvious.
Detailed description of the invention
First choose Thallus Laminariae and clean with clear water, dried and shred, being prepared into Thallus Laminariae fragment, the most by weight
Number meter, weighs 45～50 parts of mass fraction 80% ethanol, 3～5 parts of tea polyphenols and 20～25 parts of Thallus Laminariae fragments respectively and is placed in group
Knit in bruisher, smash process 2～3min to pieces；After having smashed to pieces, collect Thallus Laminariae fragment serosity, to its sucking filtration and collect filtrate
With filter cake, filtrate is placed at 30～40 DEG C rotation and is evaporated to the 1/5 of original volume, collect and concentrate Thallus Laminariae extracting solution, use ice vinegar
Acid for adjusting pH, to 3.0, is prepared into acidizing extracting liquid, then 1:5 by volume, is stirred with ether by the acidizing extracting liquid of above-mentioned preparation
Mixing, after extraction processes 3～5 times, merges ether phase, rotary evaporated to dryness at 30～40 DEG C, collects to obtain auximone, standby
With；The filter cake of above-mentioned collection is washed with deionized to cleaning mixture pH to 7.0 subsequently, at 65～70 DEG C, is dried 6～8h, receives
Collection dried undaria pinnatifida fiber, in mass ratio 5:1, stir mass fraction 10% cellulase aqueous solution with dried undaria pinnatifida fiber
Mixing, at 25～30 DEG C, heating in water bath 45～60min, filters subsequently and collects filtrate, rotating and be evaporated at 45～60 DEG C
The 1/5 of original volume, is prepared into hydrolysis Thallus Laminariae coagulant liquid；1:3 the most in mass ratio, by mass fraction 10% sodium hydroxide solution with
The hydrolysis Thallus Laminariae coagulant liquid stirring mixing of above-mentioned preparation, under 200～300W, sonic oscillation processes 10～15min, collects alkali
Change Thallus Laminariae coagulant liquid, count the most by weight, respectively weigh 45～50 parts alkalization Thallus Laminariae coagulant liquids, 35～40 parts go from
Auximone, 10～15 parts of Celastrus angulatus and 10～15 parts of phenethyl phenol polyoxy second prepared by sub-water, 5～10 parts of above-mentioned steps
Alkene ether is placed in conical flask, ultrasonic disperse 10～15min under 200～300W, stands 6～8h, can be prepared into a kind of nothing
Residual plant insecticide.
Example 1
First choose Thallus Laminariae and clean with clear water, dried and shred, being prepared into Thallus Laminariae fragment, the most by weight
Meter, weighs 45 parts of mass fraction 80% ethanol, 3 parts of tea polyphenols and 20 parts of Thallus Laminariae fragments respectively and is placed in tissue mashing machine, smash to pieces
Process 2min；After having smashed to pieces, collect Thallus Laminariae fragment serosity, to its sucking filtration and collect filtrate and filter cake, filtrate is placed in
Rotate at 30 DEG C and be evaporated to the 1/5 of original volume, collect and concentrate Thallus Laminariae extracting solution, regulate pH to 3.0 with glacial acetic acid, be prepared into acid
Change extracting solution, then 1:5 by volume, the acidizing extracting liquid of above-mentioned preparation is mixed with ether stirring, after extraction processes 3 times, close
And ether phase, rotary evaporated to dryness at 30 DEG C, collect to obtain auximone, standby；Subsequently the filter cake of above-mentioned collection is spent
Ionized water washs to cleaning mixture pH to 7.0, is dried 6h, collects dried undaria pinnatifida fiber, in mass ratio 5:1, by matter at 65 DEG C
Amount mark 10% cellulase aqueous solution mixes with the stirring of dried undaria pinnatifida fiber, at 25 DEG C, and heating in water bath 45min, subsequently mistake
Filter and collect filtrate, rotating at 45 DEG C and be evaporated to the 1/5 of original volume, being prepared into hydrolysis Thallus Laminariae coagulant liquid；The most in mass ratio
1:3, stirs mass fraction 10% sodium hydroxide solution with the hydrolysis Thallus Laminariae coagulant liquid of above-mentioned preparation and mixes, super under 200W
Sound oscillation processes 10min, collects alkalization Thallus Laminariae coagulant liquid, counts the most by weight, weighs 45 parts of alkalization Thallus Laminariae respectively
Auximone, 10 parts of Celastrus angulatus and 10 parts of phenethyl phenol prepared by coagulant liquid, 35 parts of deionized waters, 5 parts of above-mentioned steps gather
Oxygen vinyl Ether is placed in conical flask, ultrasonic disperse 10min under 200W, stands 6h, can be prepared into a kind of noresidue plant
Insecticide.
This example operation is easy, during use, is parasite killing medicinal liquid in the ratio dilute of insecticide and water proportion 1:800,
Being sprayed directly on by parasite killing medicinal liquid on the plants such as fruit tree, crops and vegetable, spray survival dose is with every mu of 30kg parasite killing medicinal liquid.
After testing, the insect pest of the noresidue vegetable insecticide energy effectively preventing plant that the present invention prepares, and materials safety environmental protection, dispenser
The release of rear drug effect is fast, easily decomposes, and does not retains residual hazard on plant, and after using 1 day, insecticidal effect reaches 93%, and the plant of the present invention kills
Worm agent after administration 35 days time, still keep higher preventive effect, the lasting period of insecticide is long.
Example 2
First choose Thallus Laminariae and clean with clear water, dried and shred, being prepared into Thallus Laminariae fragment, the most by weight
Meter, weighs 48 parts of mass fraction 80% ethanol, 4 parts of tea polyphenols and 23 parts of Thallus Laminariae fragments respectively and is placed in tissue mashing machine, smash to pieces
Process 2.5min；After having smashed to pieces, collect Thallus Laminariae fragment serosity, to its sucking filtration and collect filtrate and filter cake, filtrate is put
Rotate at 35 DEG C and be evaporated to the 1/5 of original volume, collect and concentrate Thallus Laminariae extracting solution, regulate pH to 3.0 with glacial acetic acid, be prepared into
Acidizing extracting liquid, then 1:5 by volume, mix the acidizing extracting liquid of above-mentioned preparation with ether stirring, after extraction processes 4 times,
Merge ether phase, rotary evaporated to dryness at 35 DEG C, collect to obtain auximone, standby；Subsequently the filter cake of above-mentioned collection is used
Deionized water wash, to cleaning mixture pH to 7.0, is dried 7h at 68 DEG C, collects dried undaria pinnatifida fiber, in mass ratio 5:1, will
Mass fraction 10% cellulase aqueous solution mixes with the stirring of dried undaria pinnatifida fiber, at 28 DEG C, and heating in water bath 53min, subsequently
Filter and collect filtrate, rotating at 53 DEG C and be evaporated to the 1/5 of original volume, being prepared into hydrolysis Thallus Laminariae coagulant liquid；Again by quality
Ratio 1:3, stirs mass fraction 10% sodium hydroxide solution with the hydrolysis Thallus Laminariae coagulant liquid of above-mentioned preparation and mixes, under 250W
Sonic oscillation processes 13min, collects alkalization Thallus Laminariae coagulant liquid, counts the most by weight, weighs 48 parts of alkalization opotisms respectively
Auximone, 13 parts of Celastrus angulatus and 13 parts of phenethyl phenol prepared by dish coagulant liquid, 38 parts of deionized waters, 8 parts of above-mentioned steps
Polyoxyethylene ether is placed in conical flask, ultrasonic disperse 13min under 250W, stands 7h, can be prepared into a kind of noresidue and plant
Thing insecticide.
This example operation is easy, during use, is parasite killing medicinal liquid in the ratio dilute of insecticide and water proportion 1:900,
Being sprayed directly on by parasite killing medicinal liquid on the plants such as fruit tree, crops and vegetable, spray survival dose is with every mu of 33kg parasite killing medicinal liquid.
After testing, the insect pest of the noresidue vegetable insecticide energy effectively preventing plant that the present invention prepares, and materials safety environmental protection, dispenser
The release of rear drug effect is fast, easily decomposes, and does not retains residual hazard on plant, and after using 2 days, insecticidal effect reaches 94%, and the plant of the present invention kills
Worm agent after administration 38 days time, still keep higher preventive effect, the lasting period of insecticide is long.
Example 3
First choose Thallus Laminariae and clean with clear water, dried and shred, being prepared into Thallus Laminariae fragment, the most by weight
Meter, weighs 50 parts of mass fraction 80% ethanol, 5 parts of tea polyphenols and 25 parts of Thallus Laminariae fragments respectively and is placed in tissue mashing machine, smash to pieces
Process 3min；After having smashed to pieces, collect Thallus Laminariae fragment serosity, to its sucking filtration and collect filtrate and filter cake, filtrate is placed in
Rotate at 40 DEG C and be evaporated to the 1/5 of original volume, collect and concentrate Thallus Laminariae extracting solution, regulate pH to 3.0 with glacial acetic acid, be prepared into acid
Change extracting solution, then 1:5 by volume, the acidizing extracting liquid of above-mentioned preparation is mixed with ether stirring, after extraction processes 5 times, close
And ether phase, rotary evaporated to dryness at 40 DEG C, collect to obtain auximone, standby；Subsequently the filter cake of above-mentioned collection is spent
Ionized water washs to cleaning mixture pH to 7.0, is dried 8h, collects dried undaria pinnatifida fiber, in mass ratio 5:1, by matter at 70 DEG C
Amount mark 10% cellulase aqueous solution mixes with the stirring of dried undaria pinnatifida fiber, at 30 DEG C, and heating in water bath 60min, subsequently mistake
Filter and collect filtrate, rotating at 60 DEG C and be evaporated to the 1/5 of original volume, being prepared into hydrolysis Thallus Laminariae coagulant liquid；The most in mass ratio
1:3, stirs mass fraction 10% sodium hydroxide solution with the hydrolysis Thallus Laminariae coagulant liquid of above-mentioned preparation and mixes, super under 300W
Sound oscillation processes 15min, collects alkalization Thallus Laminariae coagulant liquid, counts the most by weight, weighs 50 parts of alkalization Thallus Laminariae respectively
Auximone, 15 parts of Celastrus angulatus and 15 parts of phenethyl phenol prepared by coagulant liquid, 40 parts of deionized waters, 10 parts of above-mentioned steps gather
Oxygen vinyl Ether is placed in conical flask, ultrasonic disperse 15min under 300W, stands 8h, can be prepared into a kind of noresidue plant
Insecticide.
This example operation is easy, during use, is parasite killing medicinal liquid in the ratio dilute of insecticide and water proportion 1:1000,
Being sprayed directly on by parasite killing medicinal liquid on the plants such as fruit tree, crops and vegetable, spray survival dose is with every mu of 35kg parasite killing medicinal liquid.
After testing, the insect pest of the noresidue vegetable insecticide energy effectively preventing plant that the present invention prepares, and materials safety environmental protection, dispenser
The release of rear drug effect is fast, easily decomposes, and does not retains residual hazard on plant, and after using 3 days, insecticidal effect reaches 95%, and the plant of the present invention kills
Worm agent after administration 40 days time, still keep higher preventive effect, the lasting period of insecticide is long.
Claims (1)
1. the method that a Thallus Laminariae prepares noresidue vegetable insecticide, it is characterised in that concrete preparation process is:
(1) choose Thallus Laminariae and clean with clear water, dried and shred, being prepared into Thallus Laminariae fragment, the most by weight
Meter, weighs 45～50 parts of mass fraction 80% ethanol, 3～5 parts of tea polyphenols and 20～25 parts of Thallus Laminariae fragments respectively and is placed in tissue and smashes
In broken machine, smash process 2～3min to pieces；
(2) after having smashed to pieces, collect Thallus Laminariae fragment serosity, to its sucking filtration and collect filtrate and filter cake, filtrate is placed in 30
～rotation is evaporated to the 1/5 of original volume at 40 DEG C, collects and concentrate Thallus Laminariae extracting solution, regulate pH to 3.0 with glacial acetic acid, be prepared into
Acidizing extracting liquid, then 1:5 by volume, mix the acidizing extracting liquid of above-mentioned preparation with ether stirring, and extraction processes 3～5 times
After, merge ether phase, rotary evaporated to dryness at 30～40 DEG C, collect to obtain auximone, standby；
(3) filter cake of above-mentioned collection is washed with deionized to cleaning mixture pH to 7.0, at 65～70 DEG C, is dried 6～8h, receives
Collection dried undaria pinnatifida fiber, in mass ratio 5:1, stir mass fraction 10% cellulase aqueous solution with dried undaria pinnatifida fiber
Mixing, at 25～30 DEG C, heating in water bath 45～60min, filters subsequently and collects filtrate, rotating and be evaporated at 45～60 DEG C
The 1/5 of original volume, is prepared into hydrolysis Thallus Laminariae coagulant liquid；
(4) 1:3 in mass ratio, by the hydrolysis Thallus Laminariae coagulant liquid stirring of mass fraction 10% sodium hydroxide solution with above-mentioned preparation
Mixing, under 200～300W, sonic oscillation processes 10～15min, collects alkalization Thallus Laminariae coagulant liquid, the most by weight
Meter, weighs plant prepared by 45～50 parts of alkalization Thallus Laminariae coagulant liquids, 35～40 parts of deionized waters, 5～10 parts of steps (2) respectively
Auxin, 10～15 parts of Celastrus angulatus and 10～15 parts of phenethyl phenol polyethenoxy ethers are placed in conical flask, 200～300W
Lower ultrasonic disperse 10～15min, stands 6～8h, can be prepared into a kind of noresidue vegetable insecticide.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018174239A1 (en) * | 2017-03-24 | 2018-09-27 | 中越パルプ工業株式会社 | Agent for plant growth |
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| CN1473798A (en) * | 2003-06-26 | 2004-02-11 | 中国海洋大学 | Process for producing seaweed liquid fertilizer |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2018174239A1 (en) * | 2017-03-24 | 2018-09-27 | 中越パルプ工業株式会社 | Agent for plant growth |
| JPWO2018174239A1 (en) * | 2017-03-24 | 2019-11-21 | 中越パルプ工業株式会社 | Plant cultivation agent |
| US11490616B2 (en) | 2017-03-24 | 2022-11-08 | Chuetsu Pulp & Paper Co., Ltd | Plant growth promoting agent |
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