CN106305621A - High-yield incubation method for Yellow-margined Box Turtle - Google Patents
High-yield incubation method for Yellow-margined Box Turtle Download PDFInfo
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- CN106305621A CN106305621A CN201610756454.0A CN201610756454A CN106305621A CN 106305621 A CN106305621 A CN 106305621A CN 201610756454 A CN201610756454 A CN 201610756454A CN 106305621 A CN106305621 A CN 106305621A
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- 238000011534 incubation Methods 0.000 title claims abstract description 41
- 241001194819 Cuora flavomarginata Species 0.000 title claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 36
- 238000002156 mixing Methods 0.000 claims abstract description 12
- 230000001954 sterilising effect Effects 0.000 claims abstract description 6
- 230000012447 hatching Effects 0.000 claims description 126
- 210000004602 germ cell Anatomy 0.000 claims description 18
- 239000000284 extract Substances 0.000 claims description 15
- 239000003814 drug Substances 0.000 claims description 12
- 229940070527 tourmaline Drugs 0.000 claims description 12
- 229910052613 tourmaline Inorganic materials 0.000 claims description 12
- 239000011032 tourmaline Substances 0.000 claims description 12
- 241000143060 Americamysis bahia Species 0.000 claims description 8
- 240000007124 Brassica oleracea Species 0.000 claims description 8
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 claims description 8
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 claims description 8
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- -1 compound vitamin Chemical class 0.000 claims description 7
- 229940088594 vitamin Drugs 0.000 claims description 7
- 229930003231 vitamin Natural products 0.000 claims description 7
- 235000013343 vitamin Nutrition 0.000 claims description 7
- 239000011782 vitamin Substances 0.000 claims description 7
- 238000010792 warming Methods 0.000 claims description 7
- 239000000706 filtrate Substances 0.000 claims description 4
- 239000012467 final product Substances 0.000 claims description 4
- 239000008188 pellet Substances 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 238000002791 soaking Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 6
- 239000011159 matrix material Substances 0.000 abstract 10
- 241000208689 Eucommia ulmoides Species 0.000 abstract 1
- 229910052902 vermiculite Inorganic materials 0.000 abstract 1
- 235000019354 vermiculite Nutrition 0.000 abstract 1
- 239000010455 vermiculite Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 3
- 238000011533 pre-incubation Methods 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 241000270708 Testudinidae Species 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 241001609368 Acamptopappus Species 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 241000270693 Chinemys Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 230000008175 fetal development Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a high-yield incubation method for a Yellow-margined Box Turtle. The method comprises the steps of 1, blending vermiculite, nanometer ceramsite and eucommia ulmoides according to a mass ratio of 10:8:1, sterilizing, obtaining an incubation matrix, paving a first layer of incubation matrix with a thickness of 7-8 cm on the undersurface of an incubator, placing the oosperm on the surface of the first layer of incubation matrix, then paving the second layer of incubation matrix with the thickness of 3 cm on the oosperm; 2, during 1-8 days of the incubation, keeping water content of the first layer of incubation matrix at 10% and keeping water content of the second layer of incubation matrix at 9%; during 9-28 days of the incubation, keeping water content of the first layer of incubation matrix at 15% and keeping water content of the second layer of incubation matrix at at 15%; during the 29th day and afterwards, keeping water content of the first layer of incubation matrix at 18% and keeping water content of the second layer of incubation matrix at 20%. The high-yield incubation method for the Yellow-margined Box Turtle has the advantages of high hatchability and short incubation period.
Description
Technical field
The present invention relates to aquaculture field.It is more particularly related to the high yield side of hatching of a kind of Cistoclemmys flavomarginata
Method.
Background technology
Cistoclemmys flavomarginata, popular name, also known as food Serpentis Testudinis, China box Testudinis, golden head tortoise, is belonging to fresh water Testudinidae, box Chinemys, existing south
China seed, Taiwan kind, the big population of Ryukyu Islands kind three.Cistoclemmys flavomarginata belongs to subaquatic (the most terrestrial property) and in nature, dwells
Cease among the border in Hills, weeds, shrub, under tree root, crack of stone etc. the most local.Diel movement rule
Different with season.Belong to mountain Testudinis, it is impossible to live in deepwater field.There is problem below in Cistoclemmys flavomarginata population at present: range of activity
Little, inbred is bred, and variety deterioration is serious, laying rate, rate of fertilization, incubation rate Testudinis mortality rate low, young is high, and the speed of growth is delayed
Slowly, disease resistance is weak.
Summary of the invention
It is an object of the invention to solve at least the above, and the advantage that at least will be described later is provided.
It is a still further object of the present invention to provide the high yield incubation method of a kind of Cistoclemmys flavomarginata, its incubation rate is high, the incubation period
Short.
In order to realize according to object of the present invention and further advantage, it is provided that the high yield side of hatching of a kind of Cistoclemmys flavomarginata
Method, including:
1) 10:8:1 in mass ratio is by Vermiculitum, nanometer haydite, Cortex Eucommiae mixing, sterilizing, obtains hatching medium, uses hatching medium
Spreading ground floor hatching medium bottom incubator, thickness is 7-8cm, and germ cell is put in described ground floor hatching medium table
Face, then covers second layer hatching medium with hatching medium on germ cell, and thickness is 3cm;
2) hatching the 1-8 days, the water content controlling ground floor hatching medium is maintained at 10%, containing of second layer hatching medium
Water quantity holding is 9%;
Hatch the 9-28 days, control ground floor hatching medium water content and be maintained at 15%, second layer hatching medium aqueous
Amount is maintained at 15%;
After hatching the 29th day, control ground floor hatching medium water content and be maintained at 18%, second layer hatching medium aqueous
Amount is maintained at 20%;
Whole incubation cycle, if the female Testudinis that need to hatch accounts for larger proportion, then at the 1-8 days of hatching, controls incubator
Temperature be 33 DEG C;Hatching the 9-28 days, the temperature controlling incubator is cooled to 30 DEG C with the speed of 1 DEG C/day;Hatch the 29th day
After, control incubator temperature and be directly warming up to 32 DEG C;
If the male Testudinis that need to hatch accounts for larger proportion, then at the 1-8 days of hatching, the temperature controlling incubator is 27 DEG C;
Hatching the 9-28 days, the temperature controlling incubator is cooled to 25 DEG C with the speed of 1 DEG C/day;After hatching the 29th day, control incubator
Temperature is directly warming up to 27 DEG C.
Preferably, in described hatching medium, the length of Vermiculitum, nanometer haydite and Cortex Eucommiae controls at 0.5-1mm.
Preferably, hatching the 10th day-25 days, on the second layer hatching medium in incubator, uniformly placement is several electrically
Stone, and tourmaline do not contacts with germ cell.
Preferably, the submerge degree of depth of second layer hatching medium of described tourmaline is 0.3-0.5cm.
Preferably, special feed is used in hatching 1 month after children Testudinis: include that mass ratio is 30:8:1:15:3's
Dried small shrimps, Concha Ostreae powder, Chinese medicine extract, purple cabbage and compound vitamin;After dried small shrimps, Chinese medicine extract and purple cabbage are mixed, stir
Broken, add compound vitamin and Concha Ostreae powder mixing, add water and be kneaded into the pellet of a diameter of 2-3mm;
Described Chinese medicine extract preparation method is: take Folium Phyllanthi, adds the water soaking 30min of its weight 10 times amount, returns
Stream extracts 3h, takes filtrate and is concentrated in vacuo to 0.6g/ml, to obtain final product.
Preferably, during whole hatching, the air humidity of incubator is maintained at 86%.
The present invention at least includes following beneficial effect:
In hatching medium, add the nanometer haydite of special ratios and Cortex Eucommiae both can guarantee that the effect of constant temperature and humidity, additionally
It also has the problem that suppression germ cell is mouldy.
Hatching the 10th day-25 days, place tourmaline at second layer hatching medium and combine the hatching medium of the present invention, have
Promote the effect of fetal development, and it is the most healthy and the strongest to shorten the sub-Testudinis after brooding time, within the 29th day, directly heat up in addition combined with hatching
To assigned temperature, rather than progressively heating up, its incubation rate is higher than the incubation rate after gradually heating up.
The sub-Testudinis 1 month employing special feed after hatching, has health invigorating, the infringement of prevention bacterial disease, improves
The survival rate of sub-Testudinis.
Part is embodied by the further advantage of the present invention, target and feature by description below, and part also will be by this
Invention research and practice and be understood by the person skilled in the art.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, to make those skilled in the art with reference to description
Word can be implemented according to this.
It should be noted that experimental technique described in following embodiment, if no special instructions, it is conventional method, institute
State reagent and material, if no special instructions, the most commercially obtain.
Embodiment 1
A kind of high yield incubation method of Cistoclemmys flavomarginata, including:
1) 10:8:1 in mass ratio is by Vermiculitum, nanometer haydite, Cortex Eucommiae mixing, sterilizing, obtains hatching medium, uses hatching medium
Spreading ground floor hatching medium bottom incubator, thickness is 7cm, and germ cell is put in described ground floor hatching medium surface,
Then covering second layer hatching medium on germ cell with hatching medium, thickness is 3cm;
2) hatching the 1-8 days, the water content controlling ground floor hatching medium is maintained at 10%, containing of second layer hatching medium
Water quantity holding is 9%;
Hatch the 9-28 days, control ground floor hatching medium water content and be maintained at 15%, second layer hatching medium aqueous
Amount is maintained at 15%;
After hatching the 29th day, control ground floor hatching medium water content and be maintained at 18%, second layer hatching medium aqueous
Amount is maintained at 20%;
The female Testudinis that preincubation in the present embodiment goes out accounts for larger proportion, at the 1-8 days of hatching, controls the temperature of incubator
It it is 33 DEG C;Hatching the 9-28 days, the temperature controlling incubator is cooled to 30 DEG C with the speed of 1 DEG C/day;After hatching the 29th day, control
Incubator temperature processed is directly warming up to 32 DEG C;
Utilizing method 50 pieces of germ cell of hatching of this embodiment, incubation rate is 98%, and the sub-Testudinis of hatching 100% is female,
The average percentage hatch phase was at 68 days.
Embodiment 2
A kind of high yield incubation method of Cistoclemmys flavomarginata, including:
1) 10:8:1 in mass ratio is by Vermiculitum, nanometer haydite, Cortex Eucommiae mixing, sterilizing, obtains hatching medium, described hatching base
In matter, the length of Vermiculitum, nanometer haydite and Cortex Eucommiae controls at 1mm.Bottom incubator, ground floor hatching is spread with hatching medium
Substrate, thickness is 8cm, germ cell is put in described ground floor hatching medium surface, then with hatching medium in germ cell overlying
Lid second layer hatching medium, thickness is 3cm;
2) hatching the 1-8 days, the water content controlling ground floor hatching medium is maintained at 10%, containing of second layer hatching medium
Water quantity holding is 9%;
Hatch the 9-28 days, control ground floor hatching medium water content and be maintained at 15%, second layer hatching medium aqueous
Amount is maintained at 15%;
After hatching the 29th day, control ground floor hatching medium water content and be maintained at 18%, second layer hatching medium aqueous
Amount is maintained at 20%;
The male Testudinis that preincubation in the present embodiment goes out accounts for larger proportion, at the 1-8 days of hatching, controls the temperature of incubator
It it is 27 DEG C;Hatching the 9-28 days, the temperature controlling incubator is cooled to 25 DEG C with the speed of 1 DEG C/day;After hatching the 29th day, control
Incubator temperature processed is directly warming up to 27 DEG C, and during whole hatching, the air humidity of incubator is maintained at 86%.
In the high yield incubation method of Cistoclemmys flavomarginata, hatch the 10th day-25 days, the second layer hatching medium in incubator
The most uniformly place several tourmaline, and tourmaline does not contacts with germ cell.Described tourmaline submerges the deep of second layer hatching medium
Degree is 0.5cm.
In the high yield incubation method of Cistoclemmys flavomarginata, use special feed in hatching 1 month after children Testudinis: include quality
Than dried small shrimps, Concha Ostreae powder, Chinese medicine extract, purple cabbage and compound vitamin for 30:8:1:15:3;By dried small shrimps, Chinese medicine extract
After mixing with purple cabbage, blend, add compound vitamin and Concha Ostreae powder mixing, add water and be kneaded into the pellet of a diameter of 3mm.
Described Chinese medicine extract preparation method is: take Folium Phyllanthi, adds the water soaking 30min of its weight 10 times amount, returns
Stream extracts 3h, takes filtrate and is concentrated in vacuo to 0.6g/ml, to obtain final product.
Utilizing method 100 pieces of germ cell of hatching of this embodiment, incubation rate is 97%, and the sub-Testudinis of hatching 96% is male,
The average percentage hatch phase, after one month, sub-Testudinis survival rate was 99% at 72 days.
Comparative example
A kind of high yield incubation method of Cistoclemmys flavomarginata, including:
1) 10:8:1 in mass ratio is by Vermiculitum, nanometer haydite, Cortex Eucommiae mixing, sterilizing, obtains hatching medium, described hatching base
In matter, the length of Vermiculitum, nanometer haydite and Cortex Eucommiae controls at 1mm.Bottom incubator, ground floor hatching is spread with hatching medium
Substrate, thickness is 8cm, germ cell is put in described ground floor hatching medium surface, then with hatching medium in germ cell overlying
Lid second layer hatching medium, thickness is 3cm;
2) hatching the 1-8 days, the water content controlling ground floor hatching medium is maintained at 10%, containing of second layer hatching medium
Water quantity holding is 9%;
Hatch the 9-28 days, control ground floor hatching medium water content and be maintained at 15%, second layer hatching medium aqueous
Amount is maintained at 15%;
After hatching the 29th day, control ground floor hatching medium water content and be maintained at 18%, second layer hatching medium aqueous
Amount is maintained at 20%;
The male Testudinis that preincubation in the present embodiment goes out accounts for larger proportion, at the 1-8 days of hatching, controls the temperature of incubator
It it is 27 DEG C;Hatching the 9-28 days, the temperature controlling incubator is cooled to 25 DEG C with the speed of 1 DEG C/day;After hatching the 29th day, control
Incubator temperature processed is warming up to 27 DEG C with the speed of 0.5 DEG C/day, and during whole hatching, the air humidity of incubator is maintained at
86%.
In the high yield incubation method of Cistoclemmys flavomarginata, hatch the 10th day-25 days, the second layer hatching medium in incubator
The most uniformly place several tourmaline, and tourmaline does not contacts with germ cell.Described tourmaline submerges the deep of second layer hatching medium
Degree is 0.5cm.
In the high yield incubation method of Cistoclemmys flavomarginata, use special feed in hatching 1 month after children Testudinis: include quality
Than dried small shrimps, Concha Ostreae powder, Chinese medicine extract, purple cabbage and compound vitamin for 30:8:1:15:3;By dried small shrimps, Chinese medicine extract
After mixing with purple cabbage, blend, add compound vitamin and Concha Ostreae powder mixing, add water and be kneaded into the pellet of a diameter of 2-3mm;
Described Chinese medicine extract preparation method is: take Folium Phyllanthi, adds the water soaking 30min of its weight 10 times amount, returns
Stream extracts 3h, takes filtrate and is concentrated in vacuo to 0.6g/ml, to obtain final product.
Utilizing method 100 pieces of germ cell of hatching of this comparative example, incubation rate is 90%, and the sub-Testudinis of hatching 95% is male,
The average percentage hatch phase, after one month, the survival rate of sub-Testudinis was 97% at 72 days.
Although embodiment of the present invention are disclosed as above, but it is not restricted in description and embodiment listed
Using, it can be applied to various applicable the field of the invention completely, for those skilled in the art, and can be easily
Realizing other amendment, therefore under the general concept limited without departing substantially from claim and equivalency range, the present invention does not limit
In specific details with shown here as the embodiment with description.
Claims (6)
1. the high yield incubation method of a Cistoclemmys flavomarginata, it is characterised in that including:
1) 10:8:1 in mass ratio is by Vermiculitum, nanometer haydite, Cortex Eucommiae mixing, sterilizing, obtains hatching medium, is incubating with hatching medium
Changing bottom portion and spread ground floor hatching medium, thickness is 7-8cm, germ cell is put in described ground floor hatching medium surface, connects
And cover second layer hatching medium on germ cell with hatching medium, thickness is 3cm;
2) hatching the 1-8 days, the water content controlling ground floor hatching medium is maintained at 10%, the water content of second layer hatching medium
It is maintained at 9%;
Hatching the 9-28 days, control ground floor hatching medium water content and be maintained at 15%, the water content of second layer hatching medium is protected
Hold 15%;
After hatching the 29th day, controlling ground floor hatching medium water content and be maintained at 18%, the water content of second layer hatching medium is protected
Hold 20%;
Whole incubation cycle, if the female Testudinis that need to hatch accounts for larger proportion, then at the 1-8 days of hatching, controls the temperature of incubator
Degree is 33 DEG C;Hatching the 9-28 days, the temperature controlling incubator is cooled to 30 DEG C with the speed of 1 DEG C/day;After hatching the 29th day,
Control incubator temperature and be directly warming up to 32 DEG C;
If the male Testudinis that need to hatch accounts for larger proportion, then at the 1-8 days of hatching, the temperature controlling incubator is 27 DEG C;Hatching
The 9-28 days, the temperature controlling incubator was cooled to 25 DEG C with the speed of 1 DEG C/day;After hatching the 29th day, control incubator temperature
Directly it is warming up to 27 DEG C.
2. the high yield incubation method of Cistoclemmys flavomarginata as claimed in claim 1, it is characterised in that Vermiculitum in described hatching medium,
The length of nanometer haydite and Cortex Eucommiae controls at 0.5-1mm.
3. the high yield incubation method of Cistoclemmys flavomarginata as claimed in claim 1, it is characterised in that hatch the 10th day-25 days, incubating
Change and on the second layer hatching medium in case, uniformly place several tourmaline, and tourmaline does not contacts with germ cell.
4. the high yield incubation method of Cistoclemmys flavomarginata as claimed in claim 3, it is characterised in that described tourmaline submerges the second layer
The degree of depth of hatching medium is 0.3-0.5cm.
5. the high yield incubation method of Cistoclemmys flavomarginata as claimed in claim 1, it is characterised in that hatch 1 month after children Testudinis
Interior employing special feed: include that mass ratio is the dried small shrimps of 30:8:1:15:3, Concha Ostreae powder, Chinese medicine extract, purple cabbage and compound dimension
Raw element;After dried small shrimps, Chinese medicine extract and purple cabbage are mixed, blend, add compound vitamin and Concha Ostreae powder mixing, add water and be kneaded into
The pellet of a diameter of 2-3mm;
Described Chinese medicine extract preparation method is: take Folium Phyllanthi, adds the water soaking 30min of its weight 10 times amount, and backflow carries
Take 3h, take filtrate and be concentrated in vacuo to 0.6g/ml, to obtain final product.
6. the high yield incubation method of Cistoclemmys flavomarginata as claimed in claim 1, it is characterised in that during whole hatching, hatching
The air humidity of case is maintained at 86%.
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CN105794722A (en) * | 2016-04-06 | 2016-07-27 | 王齐东 | Industrialized cuora flavomarginata larvae breeding method |
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2016
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US4893585A (en) * | 1986-11-06 | 1990-01-16 | Ethyl Corporation | Zeolites in poultry hatching |
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Application publication date: 20170111 |