CN106281968A - Sptting plate, reaction platform and nucleic acid molecular cross instrument - Google Patents
Sptting plate, reaction platform and nucleic acid molecular cross instrument Download PDFInfo
- Publication number
- CN106281968A CN106281968A CN201610843652.0A CN201610843652A CN106281968A CN 106281968 A CN106281968 A CN 106281968A CN 201610843652 A CN201610843652 A CN 201610843652A CN 106281968 A CN106281968 A CN 106281968A
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- China
- Prior art keywords
- reactive tank
- liquid
- positive displacement
- control circuit
- controlled valve
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
Abstract
The present invention relates to technical field of medical instruments, disclose a kind of Sptting plate, including: plate body and the some reactive tanks being formed on plate body, also include: positive displacement pump and control circuit, it is provided with liquid-leaking nozzle in each described reactive tank, each liquid-leaking nozzle connects leakage pipe, on each leakage pipe, electrically-controlled valve is installed, described leakage pipe connected drainage pump, described electrically-controlled valve and described positive displacement pump connect described control circuit, described control circuit is used for opening electrically-controlled valve, and controls the liquid in positive displacement pump sucking-off institute reactive tank.Also disclose a kind of reaction platform and nucleic acid molecular cross instrument.The Sptting plate of the present invention uses the mode of leakage to discharge the residual liquid in reactive tank so that the waste liquid in reactive tank is quickly discharged and do not remains, and does not interferes with reaction precision next time.
Description
Technical field
The present invention relates to technical field of medical instruments, particularly to a kind of Sptting plate, reaction platform and making nucleic acid molecular hybridization
Instrument.
Background technology
It is widely used in industry, chemical laboratory and molecular biosciences laboratory, to participation at present at the full-automatic liquid platform that moves
Employing syringe principle pin or the tip head mostly of the liquid of reaction are to reactive tank fluid injection, or siphon away the waste liquid in reactive tank.
If fluid injection and imbibition are same pin, want detergent line during imbibition, when i.e. increasing operational simultaneously
Between expense.And owing to needle point is little with the contact area at the bottom of reactive tank, be not easy to blot in clean reactive tank when with pin imbibition
Liquid, easily under the influence of the precision once tested.
Summary of the invention
The invention provides a kind of Sptting plate and nucleic acid molecular cross instrument, solve traditional syringe needle imbibition and cannot blot clean
Problem.
A kind of Sptting plate of the present invention, including: plate body and the some reactive tanks being formed on plate body, also include: positive displacement pump
And control circuit, it being provided with liquid-leaking nozzle in each described reactive tank, each liquid-leaking nozzle connects leakage pipe, and each leakage pipe is installed
Electrically-controlled valve, described leakage pipe connected drainage pump, described electrically-controlled valve and described positive displacement pump is had to connect described control circuit, described control
Circuit is used for opening electrically-controlled valve, and controls the liquid in positive displacement pump sucking-off institute reactive tank.
Wherein, the number of described positive displacement pump is identical with described reactive tank number, and each leakage pipe connects different positive displacement pumps.
Wherein, each leakage pipe is connected to same positive displacement pump.
Wherein, described control circuit for once opening an electrically-controlled valve by scheduled timing, and control positive displacement pump sucking-off with
Liquid in the reactive tank that this electrically-controlled valve is corresponding.
Wherein, described liquid-leaking nozzle is positioned at described reaction tank bottom or sidewall.
Wherein, described plate body is the most removably provided with the cover plate for covering described reactive tank.
Wherein, described plate body uses PC, PP, PE material to make.
Present invention also offers a kind of reaction platform, be connected including the Sptting plate described in any of the above-described item and with described plate body
For making the agitating device that described plate body waves.
Wherein, described agitating device includes: gripper shoe, bearing, rocking arm, shaft coupling, position sensor and motor, described plate
Body is positioned in gripper shoe, and one end of gripper shoe is rotatably coupled bearing, and the other end is rotatably coupled described rocking arm, and rocking arm leads to
Cross described shaft coupling and connect described motor, described motor and the described position sensor described control circuit of connection.
Present invention also offers a kind of nucleic acid molecular cross instrument, including the reaction platform described in any of the above-described item.
The Sptting plate of the present invention uses the mode of leakage to discharge the residual liquid in reactive tank so that the waste liquid in reactive tank
Quickly discharging and do not remain, reaction next time is more accurate.
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
In having technology to describe, the required accompanying drawing used is briefly described, it should be apparent that, the accompanying drawing in describing below is only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, also may be used
To obtain other accompanying drawing according to these accompanying drawings.
Fig. 1 is the structural representation of a kind of Sptting plate of the present invention；
Fig. 2 is the structural representation of the another kind of Sptting plate of the present invention；
Fig. 3 is to add covering plate structure schematic diagram on the Sptting plate of Fig. 1；
Fig. 4 is a kind of structural representation reacting platform of the present invention.
Fig. 5 is the nucleic acid molecular cross instrument structural representation of the reaction platform including Fig. 3.
Description of reference numerals is as follows:
1, plate body；2, reactive tank；3, small opening；4, leakage pipe；5, electrically-controlled valve；6, positive displacement pump；7, cover plate；8, gripper shoe；9、
Bearing；10, rocking arm；11, shaft coupling；12, motor；13, position sensor；14, refrigerating plant；15, casing；16, topping-up pump；
17, the electrically-controlled valve between topping-up pump and injection needle；18, three-dimensional moving mechanism；19, injection needle；20, controller；21, fluid reservoir；
22, waste liquid pool；23, the electrically-controlled valve between topping-up pump and fluid reservoir.
Detailed description of the invention
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Describe, it is clear that described embodiment is only a part of embodiment of the present invention rather than whole embodiments wholely.Based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under not making creative work premise
Embodiment, broadly falls into the scope of protection of the invention.
The Sptting plate of the present embodiment as shown in Figures 1 to 3, including plate body 1 and the some reactive tanks 2 being formed on plate body 1,
Also including: positive displacement pump 6 and control circuit (not shown), being provided with liquid-leaking nozzle 3 in each reactive tank 2, each liquid-leaking nozzle 3 connects
Having leakage pipe 4, each leakage pipe 4 is provided with electrically-controlled valve 5, leakage pipe 4 connected drainage pump 6, electrically-controlled valve 5 and positive displacement pump 6 connect
Control circuit, control circuit is used for opening electrically-controlled valve 5, thus connects reactive tank 2 and positive displacement pump 6, and controls positive displacement pump 6 sucking-off institute
Liquid in reactive tank 2.Wherein, reactive tank 2 can also is that test tube shape, leakage pipe 4 are flexible pipe.
After reactant liquor in the Sptting plate of the present embodiment has reacted, start positive displacement pump 6 by control circuit, open simultaneously
Electrically-controlled valve 5, the positive displacement pump 6 residual liquid from liquid-leaking nozzle 3 sucking-off reactive tank 2 so that the waste liquid in reactive tank quickly discharge and
Do not remain, do not interfere with reaction precision next time.
The connection of positive displacement pump and leakage pipe has a following two ways:
Mode one: the number of positive displacement pump 6 is identical with reactive tank 2 number, each leakage pipe 4 connects different positive displacement pumps 6, often
Individual positive displacement pump 6 is all connected with control circuit, and control circuit can open all of electrically-controlled valve 5 simultaneously, and it is the most right to control positive displacement pump 6
Each reactive tank 2 carries out discharge opeing, and drain age velocity is faster.
Mode two: as illustrated in fig. 1 and 2, each leakage pipe 4 is connected to same positive displacement pump 6, i.e. only one of which positive displacement pump 6.
Control circuit can also open all of electrically-controlled valve 5 in theory, and this positive displacement pump 6 carries out discharge opeing to each reactive tank 2 simultaneously, but
After all electrically-controlled valve 5 are opened, the liquid-leaking nozzle of each reactive tank 2 is connected, due to the relation of air pressure, in not being each reactive tank 2
Waste liquid can get rid of.It is therefore preferred that control circuit for once being opened an electrically-controlled valve 5 by scheduled timing, and control row
Waste liquid in the reactive tank 2 that liquid pump 6 sucking-off is corresponding with this electrically-controlled valve 5.This mode draining mode is relative to mode one cost more
Low.
In the present embodiment, liquid-leaking nozzle 3 may be located at bottom reactive tank 2 (as shown in Figure 2) or sidewall (as shown in Figure 1),
During sidewall, liquid-leaking nozzle, near the position of bottom land, is more beneficial for fluid removal.
In the present embodiment, plate body 1 is the most removably provided with the cover plate 7 for covering reactive tank 2.Cover plate 7 can not be complete
Entirely cover reactive tank 2, leave small gap, in order to fluid injection in reactive tank 2, and reaction time avoid as far as possible dust or
Other impurity fall into reactive tank 2 and cause reaction precision to be forbidden, and can reduce again reactant liquor (volatile reaction as far as possible simultaneously
Liquid) the cross-contamination of differential responses groove 2 that brings of volatilization.Wherein, cover plate 7 can be a monoblock or groove one lid.
Plate body 1 uses PP, PC, PE material to make.On nucleic acid molecular cross instrument, PP, PC, PE is used to replace metal material
Sptting plate, it is to avoid metal itself and reactant generation chemical reaction, this type of material easily makes medical disposable material simultaneously, reduces
The pollution that Reusability brings.
As shown in Figure 4, present invention also offers a kind of reaction platform, including above-mentioned Sptting plate and the use that is connected with plate body 1
In the agitating device making plate 1 body wave, when reaction, plate body 1 is shaken so that reactant liquor mixes evenly, reacts more thorough
The end, more rapid.
This agitating device specifically includes: gripper shoe 8, bearing 9, rocking arm 10, shaft coupling 11, motor 12 and position sensor
13.Plate body 1 is positioned in gripper shoe 8, and one end of gripper shoe 8 is rotatably coupled bearing 9, and the other end is rotatably coupled rocking arm
10, rocking arm 10 connects motor 12, motor 12 and position sensor 13 by shaft coupling 11 and connects control circuit.Control circuit is passed through
The rotating speed of control motor 12 and rotation direction are to control sway velocity and amplitude, this agitating device simple in construction low cost.Row
During liquid, control circuit controls motor 12 and drives plate body 1 to rotate, after position sensor 13 detects the angle that plate body 1 forwards to preset,
To control circuit generation halt instruction, control circuit stops driving motor 12 after receiving halt instruction.This predetermined angular makes Lou
Fluid apertures 3 is in low level, is so more beneficial for waste liquid and discharges more quickly, does not remains, and reduces experiment risk, adds experiment accurately
Degree.This angle can be according to practical situation (such as: be positioned at bottom land according to liquid-leaking nozzle 3 or Sidewall angles is different) at position sensor
Set in 13.
Wherein, gripper shoe 8 can be the gripper shoe (arranging heating wire in Heat Conduction Material) with heating function, with control electricity
Road connects, and when some reaction needs to carry out under higher than ambient temperature, can control gripper shoe 8 by control circuit and generate heat.This is anti-
Answering and can also include refrigerating plant 14 in platform, connect control circuit, refrigerating plant 14 connects gripper shoe 8.Some reacts needs
When carrying out at temperatures below ambient, refrigerating plant 14 can be controlled by control circuit and freeze, owing to gripper shoe 8 is Heat Conduction Material
Make, according to heat transfer principle, the temperature of reactant liquor in reactive tank 2 can be reduced.Coordinate temperature sensor can realize instead
Answering the accurate control of liquid temp, specifically, temperature sensor connects control circuit, is sent extremely by the reacting liquid temperature monitored in real time
Control circuit, control circuit judges that when reacting liquid temperature reaches predetermined value, stopping gripper shoe 8 heats or refrigerating plant 14 freezes.
Reaction platform also includes waste liquid pool 22, and waste liquid pool 22 connected drainage pump 6, for containing the waste liquid that reactive tank 2 is got rid of.
Present invention also offers a kind of nucleic acid molecular cross instrument as it is shown in figure 5, include above-mentioned reaction platform, also include:
Topping-up pump 16, electrically-controlled valve 17, three-dimensional moving mechanism 18 (have only indicated the structure of the X-axis of transverse shifting, Y-axis and Z axis not in figure
Repeat again), injection needle 19, controller 20 and fluid reservoir 21.Fluid reservoir 21 connects injection needle 19, topping-up pump 16 by topping-up pump 16
With the pipeline between injection needle 19 is provided with electrically-controlled valve 17, the pipeline of topping-up pump 16 and fluid reservoir 21 is provided with electrically-controlled valve 23.Note
Liquid pin 19 is fixed on the slip-on head of three-dimensional moving mechanism 18, and this reaction platform is positioned at the casing 15 of nucleic acid molecular cross instrument,
And be positioned at below injection needle 19.Controller 20 connects electrically-controlled valve 17, topping-up pump 16 and three-dimensional moving mechanism 18, for by preset value
Controlling reservoir quantity and control the position of slip-on head, during fluid injection, controller 20 is by topping-up pump 16, electrically-controlled valve 17 and electrically-controlled valve 23
Control, make liquid in fluid reservoir 21 enter injection needle 19 from infusion, control injection needle 19 simultaneously and be directed at reactive tank 2.Sptting plate
And reaction platform is when nucleic acid molecular cross instrument, its control circuit is desirably integrated in controller 20, it is achieved centralized Control.
Nucleic acid molecular cross instrument discharge opeing including this reaction platform is more thorough, and cost is lower.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all spirit in the present invention and
Within principle, any modification, equivalent substitution and improvement etc. made, should be included within the scope of the present invention.
Claims (10)
1. a Sptting plate, including: plate body and the some reactive tanks being formed on plate body, it is characterised in that also include: positive displacement pump
And control circuit, it being provided with liquid-leaking nozzle in each described reactive tank, each liquid-leaking nozzle connects leakage pipe, and each leakage pipe is installed
Electrically-controlled valve, described leakage pipe connected drainage pump, described electrically-controlled valve and described positive displacement pump is had to connect described control circuit, described control
Circuit is used for opening electrically-controlled valve, and controls the liquid in positive displacement pump sucking-off institute reactive tank.
2. Sptting plate as claimed in claim 1, it is characterised in that the number of described positive displacement pump and described reactive tank number phase
With, each leakage pipe connects different positive displacement pumps.
3. Sptting plate as claimed in claim 1, it is characterised in that each leakage pipe is connected to same positive displacement pump.
4. Sptting plate as claimed in claim 3, it is characterised in that described control circuit for once being opened by scheduled timing
Individual electrically-controlled valve, and control the liquid in the reactive tank that positive displacement pump sucking-off is corresponding with this electrically-controlled valve.
5. the Sptting plate as according to any one of Claims 1 to 4, it is characterised in that described liquid-leaking nozzle is positioned at described reactive tank
Bottom or sidewall.
6. the Sptting plate as according to any one of Claims 1 to 4, it is characterised in that be the most removably provided with on described plate body
For covering the cover plate of described reactive tank.
7. the Sptting plate as according to any one of Claims 1 to 4, it is characterised in that described plate body uses PC, PP, PE material
Make.
8. a reaction platform, it is characterised in that include the Sptting plate according to any one of claim 1～7 and with as described in
The agitating device being used for making described plate body wave that plate body connects.
React platform the most as claimed in claim 8, it is characterised in that described agitating device includes: gripper shoe, bearing, rocking arm,
Shaft coupling, position sensor and motor, described plate body is positioned in gripper shoe, and one end of gripper shoe is rotatably coupled bearing, separately
One end is rotatably coupled described rocking arm, and rocking arm connects described motor, described motor and described position by described shaft coupling and passes
Sensor connects described control circuit.
10. a nucleic acid molecular cross instrument, it is characterised in that include reacting as claimed in claim 8 or 9 platform.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610843652.0A CN106281968B (en) | 2016-09-22 | 2016-09-22 | Reaction plate, reaction platform and nucleic acid molecular cross instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610843652.0A CN106281968B (en) | 2016-09-22 | 2016-09-22 | Reaction plate, reaction platform and nucleic acid molecular cross instrument |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106281968A true CN106281968A (en) | 2017-01-04 |
| CN106281968B CN106281968B (en) | 2019-06-14 |
Family
ID=57712986
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610843652.0A Active CN106281968B (en) | 2016-09-22 | 2016-09-22 | Reaction plate, reaction platform and nucleic acid molecular cross instrument |
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| Country | Link |
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| CN (1) | CN106281968B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110283709A (en) * | 2019-05-09 | 2019-09-27 | 广州和实生物技术有限公司 | Full-automatic nucleic acid molecular cross instrument |
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| JP2001208759A (en) * | 2000-01-24 | 2001-08-03 | Olympus Optical Co Ltd | Automatic analyzer |
| CN101833007A (en) * | 2010-02-25 | 2010-09-15 | 上海迅达医疗仪器有限公司 | Protein blot detection device |
| CN103926135A (en) * | 2014-05-09 | 2014-07-16 | 欧蒙医学诊断（中国）有限公司 | Automatic dilution device and method |
| CN103954785A (en) * | 2014-03-20 | 2014-07-30 | 欧蒙医学诊断（中国）有限公司 | Liquid system of western-blot analyzer |
| CN104007270A (en) * | 2014-03-20 | 2014-08-27 | 欧蒙医学诊断（中国）有限公司 | Biological detection analysis apparatus |
| CN206109376U (en) * | 2016-09-22 | 2017-04-19 | 周江 | Reaction plate, reaction platform and nucleic acid molecules hybridization appearance |
-
2016
- 2016-09-22 CN CN201610843652.0A patent/CN106281968B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001208759A (en) * | 2000-01-24 | 2001-08-03 | Olympus Optical Co Ltd | Automatic analyzer |
| CN101833007A (en) * | 2010-02-25 | 2010-09-15 | 上海迅达医疗仪器有限公司 | Protein blot detection device |
| CN103954785A (en) * | 2014-03-20 | 2014-07-30 | 欧蒙医学诊断（中国）有限公司 | Liquid system of western-blot analyzer |
| CN104007270A (en) * | 2014-03-20 | 2014-08-27 | 欧蒙医学诊断（中国）有限公司 | Biological detection analysis apparatus |
| CN103926135A (en) * | 2014-05-09 | 2014-07-16 | 欧蒙医学诊断（中国）有限公司 | Automatic dilution device and method |
| CN206109376U (en) * | 2016-09-22 | 2017-04-19 | 周江 | Reaction plate, reaction platform and nucleic acid molecules hybridization appearance |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110283709A (en) * | 2019-05-09 | 2019-09-27 | 广州和实生物技术有限公司 | Full-automatic nucleic acid molecular cross instrument |
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| Publication number | Publication date |
|---|---|
| CN106281968B (en) | 2019-06-14 |
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