CN110283709A - Full-automatic nucleic acid molecular cross instrument - Google Patents
Full-automatic nucleic acid molecular cross instrument Download PDFInfo
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- CN110283709A CN110283709A CN201910384674.9A CN201910384674A CN110283709A CN 110283709 A CN110283709 A CN 110283709A CN 201910384674 A CN201910384674 A CN 201910384674A CN 110283709 A CN110283709 A CN 110283709A
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- Prior art keywords
- nucleic acid
- full
- acid molecular
- automatic nucleic
- molecular cross
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
Abstract
The invention discloses a kind of full-automatic nucleic acid molecular cross instruments, belong to the field of medical instrument technology, to solve the problems, such as that existing hybridization instrument is easy to appear cross-infection and designs.The full-automatic nucleic acid molecular cross instrument of the present invention includes main case, hybridization processing platform, mechanical movement platform and sample adding system are provided in main case, full-automatic nucleic acid molecular cross instrument further includes the Mead-Bauer recovery system being isolated with sample adding system, and Mead-Bauer recovery system is connected to the reactive tank of hybridization processing platform.The full-automatic nucleic acid molecular cross instrument of the present invention enables sample-adding process and removal waste fluid process be completely separated out, and avoids the sample or test solution in the waste liquid cross-infection follow-up test of previous test, helps to reduce the cross-infection problem occurred during the test;The setting for reducing artificial parameter, simplifies, and saves manpower and time cost.
Description
Technical field
The present invention relates to the field of medical instrument technology more particularly to a kind of full-automatic nucleic acid molecular cross instruments.
Background technique
Hybridization Oven be it is a kind of using hybridization detection testing gene group in whether contain known
The equipment of sequence and a kind of in-vitro diagnosis equipment.It is generally necessary to add one or more reagents into multiple reactive tanks, adding
Heat does not react under heated condition, and the waste liquid in reactive tank is excluded after measurement to be tested.
It is mostly to be inserted drain pipe from the opening of reactive tank when existing automatic hybridization device exclusion waste liquid, utilizes pump
Waste liquid is extracted out from reactive tank by drain pipe.Wherein, drain pipe used when drain is also used as liquid-feeding tube in the product of sample-adding
It uses.
The defect of the structure is: being difficult all to extract whole waste liquids out, variable amount of raffinate (raffinate can be left after drawing liquid
It is likely located in reactive tank and/or on the inside and outside wall of drain pipe), raffinate may will affect another reaction when causing use next time
Liquid, to the problem of cross-infection occur.
Summary of the invention
It is an object of the invention to propose in a kind of waste liquid cross-infection follow-up test for being avoided that previous test sample or
The full-automatic nucleic acid molecular cross instrument of test solution.
To achieve this purpose, the present invention adopts the following technical scheme:
A kind of full-automatic nucleic acid molecular cross instrument includes main case, and hybridization processing platform, machine are provided in the main case
Tool motion platform and sample adding system, the full-automatic nucleic acid molecular cross instrument further include the waste liquid being isolated with the sample adding system
Recovery system, the Mead-Bauer recovery system are connected to the reactive tank of the hybridization processing platform.
In particular, openable and closable leakage fluid dram is offered on the bottom surface of the reactive tank, the Mead-Bauer recovery system connection
To the leakage fluid dram.
In particular, being provided with multiple groove structures on the reactive tank, offered on the bottom surface of each groove structure
One openable and closable leakage fluid dram.
In particular, the Mead-Bauer recovery system is located at the lower section of the full-automatic nucleic acid molecular cross instrument, including it is provided with
The devil liquor recovery slot of waste liquid outlet, the plug for blocking the leakage fluid dram, the waste liquid being connected on the waste liquid outlet
Pipe and motor for driving the devil liquor recovery slot to move back and forth.
In particular, the bottom surface of the devil liquor recovery slot is funnel-shaped, the waste liquid outlet is located at the funnel-shaped structure
Lowest part.
In particular, being formed with lubricant coating on the inner surface of the reactive tank.
In particular, the plug is made of plastic material.
In particular, the full-automatic nucleic acid molecular cross instrument further includes the splashproof cover board being covered on the reactive tank.
In particular, being hingedly provided with transparent window on the main case, the transparent window can be overturn along articulated section.
In particular, the hybridization processing platform further includes that heating sheet below the reactive tank is arranged in and is located at institute
State the thermal insulation board on the outside of reactive tank and the heating sheet.
In particular, the sample adding system includes liquid switching system, the liquid switching system can be controlled by solenoid valve
Make being turned on and off for different waterway pipes.
The sample adding system of the full-automatic nucleic acid molecular cross instrument of the present invention is isolated with Mead-Bauer recovery system, guarantees sample-adding process
It is completely separated out with removal waste fluid process, avoids the sample or test solution in the waste liquid cross-infection follow-up test of previous test, help
In the cross-infection problem that reduction occurs during the test;The setting for reducing artificial parameter, simplifies, save manpower and
Time cost.
Detailed description of the invention
Fig. 1 is the structural schematic diagram for the full-automatic nucleic acid molecular cross instrument that the specific embodiment of the invention provides;
Fig. 2 is the full-automatic nucleic acid molecular cross instrument after the dismantling operation transparent window that the specific embodiment of the invention provides
Structural schematic diagram;
Fig. 3 is the explosive view for the full-automatic nucleic acid molecular cross instrument that the specific embodiment of the invention provides;
Fig. 4 is matching for hybridization processing platform, Mead-Bauer recovery system and the splashproof cover board that the specific embodiment of the invention provides
Close structural schematic diagram;
Fig. 5 is the structural schematic diagram for the Mead-Bauer recovery system that the specific embodiment of the invention provides;
Fig. 6 is the explosive view for the hybridization processing platform that the specific embodiment of the invention provides;
Fig. 7 is the structural schematic diagram for the sample-adding platform that the specific embodiment of the invention provides.
In figure:
1, main case;2, hybridize processing platform;3, mechanical movement platform;4, it is loaded platform;5, Mead-Bauer recovery system;6, it touches
Touch screen operating platform;7, splashproof cover board;8, power supply;9, bottom plate;11, touch screen;12, power interface;13, operation transparent window
Mouthful;14, liquid switching system;21, reactive tank;22, heating sheet;23, thermal insulation board;41, sample needle;42, support plate;51, waste liquid
Accumulator tank;52, plug;53, sewer pipe;54, motor;211, leakage fluid dram;212, groove structure;511, waste liquid outlet.
Specific embodiment
To further illustrate the technical scheme of the present invention below with reference to the accompanying drawings and specific embodiments.
Present embodiment discloses a kind of full-automatic nucleic acid molecular cross instrument.As shown in Figures 1 to 7, full-automatic nucleic acid point
Sub- hybridization instrument includes main case 1, and hybridization processing platform 2, mechanical movement platform 3, sample adding system and waste liquid are provided in main case 1
Recovery system 5.Wherein, Mead-Bauer recovery system 5 is connected to the reactive tank 21 of hybridization processing platform 2, and sample adding system and waste liquid return
Receipts system 5 is isolated.
Sample adding system above reactive tank 21 is used to add hybond membrane item or various hybridization solutions, tool into reactive tank 21
Body, sample adding system includes solenoid valve and water pump, can be extracted different test reaction solutions from different bottles out, then by liquid feeding
Pipeline liquid feeding carries out test reaction into reactive tank 21;Mead-Bauer recovery system 5 positioned at 21 lower section of reactive tank is used for cleaning reaction
Sample adding system and Mead-Bauer recovery system 5 are designed to mutually independent structure, do not generate friendship by the waste liquid of end of reaction in slot 21
Fork, at least partly structure for solving sample adding system in existing apparatus are used for extracting waste liquid, cause to be easy in follow-up test
The problem of showing cross-infection and then leading to test failure.
As shown in figure 5,5 preferred structure of Mead-Bauer recovery system include the devil liquor recovery slot 51 for being provided with waste liquid outlet 511,
Plug 52, the sewer pipe 53 being connected on waste liquid outlet 511 for blocking leakage fluid dram 211, for driving devil liquor recovery slot
51 motors 54 and motor control module moved back and forth.
Wherein, as shown in fig. 6, being provided with multiple groove structures 212 on reactive tank 21, the bottom surface of each groove structure 212
On offer an openable and closable leakage fluid dram 211, Mead-Bauer recovery system 5 is connected to leakage fluid dram 211.In present embodiment, each
24 four rows, every row six totals groove structures 212 are preferably provided on reactive tank 21, for storing hybond membrane item and each
The groove structure 212 of kind of hybridization solution is mutually indepedent, and whole groove structures 212 can work independently in hybrid process, can also be with
It works at the same time.Reagent cross-infection in hybrid process can be prevented independently of each other between each groove structure 212, avoid influencing to try
Test result.Splashproof cover board 7 can be set on reactive tank 21, splashproof cover board 7 is covered on the opening of groove structure 212, into one
Step ground independently opens each groove structure 212, reduces each sample and leads in an experiment because rocking and slightly volatilizing because of heating
The problem of appearance cross-infection of cause.
In hybrid process, devil liquor recovery slot 51 is mutually drawn close with reactive tank 21, and plug 52 is inserted in leakage fluid dram 211, drain
Mouth 211 is blocked, and will not be leaked inside groove structure 212, can be normally carried out reaction;Every time after the completion of hybridization, motor
54 driving devil liquor recovery slots 51 are disengaged with 21 phase of reactive tank, and plug 52 is extracted out from leakage fluid dram 211, useless in groove structure 212
Liquid toward lower is flowed in devil liquor recovery slot 51 through leakage fluid dram 211 from eminence, converges to the waste liquid at waste liquid outlet 511 through useless
Liquid pipe 53 is discharged.During entire drain, waste liquid can be thoroughly discharged from groove structure 212 by gravity, be avoided
Remaining waste in groove structure 212.
The bottom surface of devil liquor recovery slot 51 is preferably in the little funnel-form of gradient, and waste liquid outlet 511 is located at funnel-shaped structure
Lowest part.Waste liquid is under gravity from highly flowing downwards when drain, guarantees smooth thoroughly to converge to waste liquid
At outlet 511, residual will not be formed in devil liquor recovery slot 51, avoids influencing subsequent test.
In order to further increase the speed and percentage of waste liquid discharge, can be formed on the inner surface of reactive tank 21 by iron
Lubricant coating made of fluorine dragon (PTFE).The funnel-shaped structure of 21 bottom surface of reactive tank, the lubricant coating of 21 inner surface of reactive tank, again
It is rocked with reactive tank 21 is closed under motor control, waste liquid can thoroughly be enabled to flow from position relatively high in reactive tank 21 very much
It is discharged to relatively low position, again from the leakage fluid dram 211 for being located at lowest part, into devil liquor recovery slot 51.
In order to avoid reaction solution leaks during cross experiment, the modeling that deformability is good and recovery capability is strong is used
Plug 52, such as silica gel is made in property material.A degree of deformation can be generated after plug 52 is inserted into leakage fluid dram 211, enables plug
52 periphery wall is tightly abutted with the inner wall of leakage fluid dram 211, and reaction solution is avoided to leak out between plug 52 and leakage fluid dram 211.
When groove structures 212 whole in hybrid process work independently, added test solution in each groove structure 212
May be different, or the sequence of addition is different.In order to control the addition content in each groove structure 212 personalizedly, it is loaded
System includes liquid switching system 14, and liquid switching system 14 can be controlled by solenoid valve (preferably four, six or eight)
Make being turned on and off for different waterway pipes.It is switched on or off specifically, controlling the two two of solenoid valve in software program, thus
Switch different waterway channels, so that different test solutions (reaction liquid) be enabled to carry out liquid feeding by the formula of setting.
On the basis of above structure, hybridization processing platform 2 further include be arranged in heating sheet 22 below reactive tank 21, with
And the thermal insulation board 23 positioned at 22 outside of reactive tank 21 and heating sheet.Heating sheet 22 can enable reagent be maintained at best work in hybrid process
Make in temperature.Thermal insulation board 23 is used to play insulation effect when heating sheet 22 heats, and enables reagent during the work time not by outside
The influence of temperature;It can also play the role of preventing inside and outside heat from exchanging simultaneously, avoiding can not because inside and outside heat exchanges
It works normally, and operator can be prevented to be burned.
On the basis of above structure, as shown in Figure 2 and Figure 7, mechanical movement platform 3 includes X-axis track, Y-axis track, Z
Axis track and motor control module, the sample-adding platform 4 in sample adding system are mounted on Z axis track.Wherein, X-axis track can pass through
Motor mechanism drives Y-axis track to move to the upper center for needing the hybridization slot of work from origin, passes through the more of Z axis orbits controlling
Channel sample-adding platform 4 moves down at the groove structure 212 for needing reagent adding, and the sample needle 41 being mounted in support plate 42 is logical
It crosses splashproof cover board 7 and is inserted into the inner reagent adding of groove structure 212;It is loaded platform 4 after reagent adding up to move, is detached from splashproof cover board
7, it prevents reagent during reagent adding to be splashed in other groove structures 212, prevents Reagent crossing.Y-axis track is fixed on hybridization
On processing platform 2, relative to hybridization 2 swing of processing platform.On the basis of above structure, it is additionally provided in main case 1
Touch screen operation platform 6, power supply 8, bottom plate 9 and circuit system control module, are separately fixed at accordingly with hexagon socket head cap screw
Screw hole on, cross experiment can be automatically performed by the automatic test pattern of equipment, reduce human cost, reduce the time at
This.
The outside of main case 1 is provided with touch screen 11, power interface 12 and operation transparent window 13, wherein touch screen
11 stick into from the front end of bottom plate 9, and the port between the line of internal circuit and line is facilitated to connect;Power interface 12 is located at host
The left side of shell 1, various routes are all connected on the left of instrument, and the right side of instrument is rather spacious, spacious, are reduced to most of habits
Make the influence of right-handed operator;Transparent window 13 for observing operations in hybrid process is hinged on main case 1,
Transparent window 13 can be overturn along articulated section, be preferably made of transparent acrylic.Operator, which can according to need, when work comes
The opening degree of transparent window 13 is adjusted, the unlatching limit of transparent window 13 can make after opening wide preferably between 0 ° to 180 °
Transparent window 13 is controlled with hydraulic mechanism to be parked at required angle.
For providing man machine operation interface, operator can be arranged by touch screen operating platform 6 to be hybridized touch screen 11
Parameter required for process by software through process control hybrid process and obtains results of hybridization;Power supply 8 is used for into complete machine
Each electric appliance provides required burning voltage;Bottom plate 9 mainly provides fixed position and support construction to accessory each in complete machine.
The full-automatic nucleic acid molecular cross instrument is integrated embedded control system, and operator can be by equipment
Touch screen operation platform 6 carries out information exchange, different control instructions can be sent by different interface buttons, to control
Corresponding module operation, realizes the functions such as molecule hybridization, washing, display.
In use, step 1. connects the reagent got ready by the pipeline of instrument, the switch of host power supply 8 is opened, rotation is saturating
Bright window 13, removes splashproof cover board 7, and the hybond membrane item of required experiment is placed in groove structure 212, splashproof cover board 7 is covered.
Step 2. closes transparent window 13, operates on touch screen operation platform 6, controls mould by software through circuit system
Block controls mechanical movement platform.Parameter required for selection operating mode and input (when can be but being not limited to hybridize
Required time etc. when temperature when amount of reagent, hybridization, hybridization).
After step 3. operator confirms operating mode, " determination " button is pressed, system can be according to setting under state without exception
The parameter set hybridizes automatically, and process is not necessarily to manual intervention.If be abnormal in the course of work, prompt tone sounds, and stops
Current to operate, " elimination " button on operator's point touching screen operating platform 6 can eliminate prompt tone;Operator is according to touching
It touches the exception (such as reagent water level is inadequate, parameter setting is incorrect etc.) shown on screen operating platform 6 to solve the problems, such as, then point again
Determination is hit, instrument can connect secondary start-up operation when stopping, without from the beginning.
System can issue prompt tone after the completion of step 4. cross experiment.Transparent window 13 can be opened at this time, remove Splashproof cover
Plate 7 takes out hybond membrane item, checks the result after hybridizing.
Step 5. closes transparent window 13, presses cancel key, and system enters automatic cleaning model, starts to groove structure
212 are cleaned, and prevent test next time from having cross-infection.After the completion of cleaning, system automatically turns on ultraviolet lamp and carries out disinfection mould
Formula.System enters standby mode after the completion of disinfection, and power supply is turned off manually in operator, completes operation.
Note that the technical principle that above are only presently preferred embodiments of the present invention and used.Those skilled in the art can manage
Solution, the invention is not limited to the specific embodiments described herein, is able to carry out various apparent changes for a person skilled in the art
Change, readjust and substitutes without departing from protection scope of the present invention.Therefore, although by above embodiments to the present invention into
It has gone and has been described in further detail, but the present invention is not limited to the above embodiments only, without departing from the inventive concept,
It can also include more other equivalent embodiments, and the scope of the invention is determined by the scope of the appended claims.
Claims (10)
1. a kind of full-automatic nucleic acid molecular cross instrument includes main case (1), it is flat that hybridization processing is provided in the main case (1)
Platform (2), mechanical movement platform (3) and sample adding system, which is characterized in that the full-automatic nucleic acid molecular cross instrument further includes and institute
The Mead-Bauer recovery system (5) that sample adding system is isolated is stated, the Mead-Bauer recovery system (5) is connected to the hybridization processing platform
(2) reactive tank (21).
2. full-automatic nucleic acid molecular cross instrument according to claim 1, which is characterized in that at the bottom of the reactive tank (21)
It is offered on face openable and closable leakage fluid dram (211), the Mead-Bauer recovery system (5) is connected to the leakage fluid dram (211).
3. full-automatic nucleic acid molecular cross instrument according to claim 2, which is characterized in that be arranged on the reactive tank (21)
Have multiple groove structures (212), offers an openable and closable leakage fluid dram on the bottom surface of each groove structure (212)
(211)。
4. full-automatic nucleic acid molecular cross instrument according to claim 2, which is characterized in that the Mead-Bauer recovery system (5)
Positioned at the lower section of the full-automatic nucleic acid molecular cross instrument, including be provided with waste liquid outlet (511) devil liquor recovery slot (51),
For block the plug (52) of the leakage fluid dram (211), the sewer pipe (53) being connected on the waste liquid outlet (511), with
And the motor (54) for driving the devil liquor recovery slot (51) to move back and forth;
Especially, the plug (52) is made of plastic material.
5. full-automatic nucleic acid molecular cross instrument according to claim 4, which is characterized in that the devil liquor recovery slot (51)
Bottom surface is funnel-shaped, and the waste liquid outlet (511) is located at the lowest part of the funnel-shaped structure.
6. full-automatic nucleic acid molecular cross instrument according to any one of claim 1 to 5, which is characterized in that described anti-
It answers and is formed with lubricant coating on the inner surface of slot (21).
7. full-automatic nucleic acid molecular cross instrument according to any one of claim 1 to 5, which is characterized in that it is described it is complete from
Dynamic nucleic acid molecular cross instrument further includes the splashproof cover board (7) being covered on the reactive tank (21).
8. full-automatic nucleic acid molecular cross instrument according to any one of claim 1 to 5, which is characterized in that in the master
It is hingedly provided on casing (1) transparent window (13), the transparent window (13) can overturn along articulated section.
9. full-automatic nucleic acid molecular cross instrument according to any one of claim 1 to 5, which is characterized in that the hybridization
Processing platform (2) further includes the heating sheet (22) being arranged below the reactive tank (21) and is located at the reactive tank (21)
With the thermal insulation board (23) on the outside of the heating sheet (22).
10. full-automatic nucleic acid molecular cross instrument according to any one of claim 1 to 5, which is characterized in that the sample-adding
System includes liquid switching system (14), and the liquid switching system (14) can control different waterway pipes by solenoid valve
It is turned on and off.
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CN201910384674.9A CN110283709A (en) | 2019-05-09 | 2019-05-09 | Full-automatic nucleic acid molecular cross instrument |
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CN201910384674.9A CN110283709A (en) | 2019-05-09 | 2019-05-09 | Full-automatic nucleic acid molecular cross instrument |
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Cited By (1)
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Inventor after: Chen Huayun Inventor after: Du Yikong Inventor after: Li Binjian Inventor after: Liu Shuyuan Inventor before: Chen Yunhua Inventor before: Du Yikong Inventor before: Li Binjian Inventor before: Liu Shuyuan |