CN206109410U - Nucleic acid molecule hybridizing apparatus - Google Patents
Nucleic acid molecule hybridizing apparatus Download PDFInfo
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- CN206109410U CN206109410U CN201621073749.XU CN201621073749U CN206109410U CN 206109410 U CN206109410 U CN 206109410U CN 201621073749 U CN201621073749 U CN 201621073749U CN 206109410 U CN206109410 U CN 206109410U
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- liquid
- controller
- nucleic acid
- acid molecular
- pump
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Abstract
The utility model relates to the technical field of medical equipment, a nucleic acid molecule hybridizing apparatus is disclosed, include: casing, reaction platform, notes liquid needle, infusion pump, liquid storage pot, temperature sensor, attemperator and controller, but the reaction platform with annotate liquid needle relative movement ground and set up in the casing, infusion pump connects and annotates liquid needle and liquid storage pot, infusion pump with annotate the pipeline that the liquid needle is connected on be equipped with first automatically controlled valve, be equipped with the automatically controlled valve of second on the pipeline between infusion pump and the liquid storage pot, temperature sensor is located the liquid storage pot, attemperator is used for heating or cooling down the liquid storage pot, controller connection attemperator, temperature sensor and infusion pump. The utility model discloses a liquid of nucleic acid molecule hybridizing apparatus's controller control attemperator in to the liquid storage pot carries out temperature regulation to receive the liquid temperature of temperature sensor determine to the liquid storage pot in, when the liquid temperature reaches the default, control infusion pump annotates the transport of liquid needle to reacting the platform with the fluid passage of liquid storage pot, thereby has realized the process of full -automatic preliminary treatment.
Description
Technical field
This utility model is related to technical field of medical instruments, more particularly to a kind of nucleic acid molecular cross instrument.
Background technology
As development of modern industry is to robot and the growing demand of automatic business processing, all trades and professions specilization
Learn, biological industry requires more and more higher to experiment automatized.
Hybridization is：Refer to two single nucleic acid strands (DNA or RNA) with certain homologous sequence, certain
Under the conditions of press base pair complementarity principle through annealing, formed heteroduplex process.Current existing making nucleic acid molecular hybridization
Instrument mostly is the semi-automatic hybridization instrument that manual operations coordinates apparatus control, operates cumbersome.Some Full-automatic hybridization appliances, there is also with
Lower problem：
The measurement and pretreatment (being heated or cooled to the temperature of liquid for needing) of temperature, liquid level before liquid injection reactive tank
The function such as high-low position alarm and temperature control.Make one of this original link for full-automatic flow process is changed into manual, makes full-automatic
Flow process is being semi-automatic to a certain degree, increased the cost of manpower.
Utility model content
The utility model proposes a kind of nucleic acid molecular cross instrument, the Full-automatic hybridization appliance for solving prior art cannot be realized
The problem of the automatic pretreatment of liquid.
A kind of nucleic acid molecular cross instrument of the present utility model, including：Casing, reaction platform, injection needle, topping-up pump, liquid storage
Tank, temperature sensor, register and controller；The reaction platform and injection needle can relatively movably be arranged on the casing
Interior, the topping-up pump connects injection needle and fluid reservoir, and the pipeline that the topping-up pump is connected with injection needle is provided with the first electrically-controlled valve,
Pipeline between the topping-up pump and fluid reservoir is provided with the second electrically-controlled valve, and the temperature sensor is located in the fluid reservoir,
The register connects the fluid reservoir, and the controller connects the register, temperature sensor and topping-up pump.
Wherein, also include：Liquid level sensor, the liquid level sensor are located in the fluid reservoir, and connect the control
Device.
Wherein, the reaction platform is fixed on bottom of shell, and injection needle is arranged on the reaction by three-dimensional moving mechanism
Above platform, the three-dimensional moving mechanism connects the controller.
Wherein, the reaction platform is arranged on the bottom of shell, the moveable platform connection by moveable platform
The controller, the injection needle are fixed on above reaction platform.
Wherein, photographic head is additionally provided with the casing, the photographic head connects the controller.
Wherein, the wireless communications chips for wireless connection host computer are also included in the controller.
Wherein, the reaction platform includes：Sptting plate and the agitating device for waving the Sptting plate, the reaction
Plate includes：Positive displacement pump, plate body and some reactive tanks being formed on plate body, are provided with liquid-leaking nozzle in each described reactive tank, each
Liquid-leaking nozzle is connected with leakage pipe, is provided with the 3rd electrically-controlled valve on each leakage pipe, the leakage pipe connected drainage pump, and the described 3rd
Electrically-controlled valve and the positive displacement pump connect the controller, and the agitating device connects the plate body.
Wherein, the agitating device includes：Gripper shoe, bearing, rocking arm, shaft coupling, position sensor and motor, the plate
Body is located in gripper shoe, and one end of gripper shoe is rotatably coupled bearing, and the other end is rotatably coupled the rocking arm, and rocking arm leads to
Cross the shaft coupling and connect the motor, the motor and the position sensor connect the controller.
Wherein, the liquid-leaking nozzle is located at the reaction tank bottom or side wall.
Wherein, also removably it is provided with the plate body for covering the cover plate of the reactive tank.
The controller control register of nucleic acid molecular cross instrument of the present utility model carries out temperature to the liquid in fluid reservoir
Degree is adjusted, and receives the temperature of liquid that temperature sensor is detected in fluid reservoir, when temperature of liquid reaches preset value, control storage
Flow container is turned on to the pipeline between injection needle, and the liquid of fluid reservoir is delivered to reaction platform by injection needle by topping-up pump, so as to
Realize the process of full-automatic pretreatment.
Description of the drawings
In order to be illustrated more clearly that this utility model embodiment or technical scheme of the prior art, below will be to embodiment
Or accompanying drawing to be used is briefly described needed for description of the prior art, it should be apparent that, drawings in the following description are only
It is some embodiments of the present utility model, for those of ordinary skill in the art, before creative labor is not paid
Put, can be with according to these other accompanying drawings of accompanying drawings acquisition.
Fig. 1 is a kind of nucleic acid molecular cross instrument structural representation of the present utility model；
Fig. 2 is a kind of structural representation of Sptting plate in the nucleic acid molecular cross instrument of Fig. 1；
Structural representations of the Fig. 3 for another kind of Sptting plate in the nucleic acid molecular cross instrument of Fig. 1.
Description of reference numerals is as follows：
1st, plate body；2nd, reactive tank；3rd, small opening；4th, leakage pipe；5th, the 3rd electrically-controlled valve；6th, positive displacement pump；7th, cover plate；8th, support
Plate；9th, bearing；10th, rocking arm；11st, shaft coupling；12nd, motor；13rd, position sensor；14th, refrigerating plant；15th, casing；16th, fluid injection
Pump；17th, the first electrically-controlled valve；18th, three-dimensional moving mechanism；19th, injection needle；20th, controller；21st, waste liquid pool；22nd, register；
23rd, fluid reservoir；24th, the second electrically-controlled valve；25th, photographic head；26 liquid level sensors；27th, temperature sensor；28th, base.
Specific embodiment
Below in conjunction with the accompanying drawing in this utility model embodiment, the technical scheme in this utility model embodiment is carried out
Clearly and completely describe, it is clear that described embodiment is only this utility model a part of embodiment, rather than whole
Embodiment.Based on the embodiment in this utility model, those of ordinary skill in the art are not under the premise of creative work is made
The every other embodiment for being obtained, belongs to the scope of this utility model protection.
As shown in figure 1, the nucleic acid molecular cross instrument of the present embodiment includes：Casing 15, reaction platform, injection needle 19, fluid injection
Pump 16, fluid reservoir 23, temperature sensor 27, register 22 (similar to the equipment of air-conditioning) and controller 20.Reaction platform and
Injection needle 19 relatively movably can be arranged in casing 15, the connection injection needle 19 and fluid reservoir 23 of topping-up pump 16, topping-up pump 16 with
The pipeline of the connection of injection needle 19 is provided with the first electrically-controlled valve 17, and it is electric that the pipeline between topping-up pump 16 and fluid reservoir 23 is provided with second
Control valve 24.Temperature sensor 27 is located in fluid reservoir 23, the connection fluid reservoir 23 of register 22, for heating to fluid reservoir 23 or
Cooling, to adjust the temperature of liquid in fluid reservoir 23, the connection register 22 of controller 20, temperature sensor 27 and topping-up pump
16。
The control register 22 of controller 20 of the nucleic acid molecular cross instrument of the present embodiment is carried out to the liquid of fluid reservoir 23
Temperature adjustment, and the temperature of liquid that temperature sensor 27 is detected in fluid reservoir is received, when temperature of liquid reaches preset value, control
Fluid reservoir processed 23 is turned on to the pipeline between injection needle 19, and the liquid of fluid reservoir 23 is delivered to by topping-up pump 16 by injection needle 19
Reaction platform, it is achieved thereby that the process of full-automatic pretreatment.Fluid reservoir 23 can be set according to the different of the liquid for participating in reaction
Put multiple, correspondingly, the number of topping-up pump 16 and injection needle 19 is identical with the quantity of fluid reservoir 23, the second electrically-controlled valve 24 can be with
It is the valve combination with 23 number identical single valve of fluid reservoir, or multiselect one.Each fluid reservoir 23 has temperature sensing
Device 27, controller 20 can pass through different input interfaces and connect different temperature sensors 27, be received by different input interfaces
Whether temperature value reaches predetermined value come the temperature for judging which fluid reservoir 23.
The nucleic acid molecular cross instrument of the present embodiment also includes：Liquid level sensor 26, the liquid level sensor 26 are located at fluid reservoir
In 23, and connect controller 20, when the liquid level that liquid is demarcated less than liquid level sensor 26, liquid level sensor 26 gives a warning,
To point out experimenter to note, while warning message is transmitted to controller 20, the control topping-up pump 16 of controller 20 stops fluid injection.
Reaction platform and injection needle 19 can relative movement include following two modes：
Mode one：Reaction platform is fixed on the bottom of casing 15, and injection needle 19 (is schemed in Fig. 1 by three-dimensional moving mechanism 18
In only indicated transverse shifting X-axis structure, Y-axis and Z axis are repeated no more) above reaction platform, specifically, note
Liquid pin 19 is arranged on the slip-on head of three-dimensional moving mechanism 18, the connection controller 20 of three-dimensional moving mechanism 18, can be in controller
The position of fluid injection is accurately positioned under 20 control.
Mode two：Reaction platform is arranged on the bottom of casing 15, moveable platform connection controller by moveable platform
20, injection needle 19 is fixed on above reaction platform.Specifically, moveable platform can be by cross track, long rails and step
Stepper motor is constituted, and motor connection controller 20, the base 28 for reacting platform are arranged on moveable platform by slide block, step
Stepper motor drives slide block under the control of controller 20 so that entirely reacting platform cross shifting so that the reaction on reaction platform
Groove is accurately positioned with injection needle 19.
In the present embodiment, photographic head 25 in casing 15, is additionally provided with, the reaction of collection is regarded by the connection controller 20 of photographic head 25
Frequency real-time Transmission is stored to controller 20, and the approval for subsequent experimental result provides foundation.
In the present embodiment, also include the wireless communications chips for wireless connection host computer in controller 20, such as：Bluetooth,
Wifi, 3G or 4G etc..Controller 20 is communicated with host computer by wireless communications chips, and host computer can send to controller 20 and control
The control instruction of the parts such as topping-up pump 16, the first electrically-controlled valve 17, the second electrically-controlled valve 24 and register 22.In addition, controller 20
Photographic head 25 is gathered into realtime video transmission to host computer, can entirely be tested by host computer remote control, while obtaining real
Result is tested, hence for there is virus, and the laboratory such as toxic and harmful and the experiment in place need to be isolated is provided and is solved
Scheme.
In the present embodiment, reaction platform includes Sptting plate and the agitating device for waving the Sptting plate.Such as Fig. 2 and
Shown in 3, Sptting plate includes：Positive displacement pump 6, plate body 1 and some reactive tanks 2 being formed on plate body 1, are provided with each reactive tank 2
Liquid-leaking nozzle 3, each liquid-leaking nozzle 3 are connected with leakage pipe 4, and the 3rd electrically-controlled valve 5, the connection row of leakage pipe 4 are provided with each leakage pipe 4
Liquid pump 6, the 3rd electrically-controlled valve 5 and the connection controller 20 of positive displacement pump 6, after the completion of experiment, it is automatically controlled that the control of controller 20 opens the 3rd
Valve 5, and control the liquid in the sucking-off of positive displacement pump 6 institute reactive tank 2.Agitating device connects plate body 1, plate body 1 is carried out in reaction
Shake so that reactant liquor mixes evenly, reaction is more thoroughly, more rapidly.
Specifically, agitating device includes：Gripper shoe 8, bearing 9, rocking arm 10, shaft coupling 11, position sensor 13 and motor
12, plate body 1 is located in gripper shoe 8, and one end of gripper shoe 8 is rotatably coupled bearing 9, and the other end is rotatably coupled rocking arm
10, rocking arm 10 connects motor 12, motor 12 and the connection controller 20 of position sensor 13 by shaft coupling 11.Controller 20 passes through
The rotating speed and rotation direction of controlled motor 12 to control sway velocity and amplitude, the agitating device simple structure low cost.In row
During liquid, 20 controlled motor of controller 12 drives plate body 1 to rotate, and position sensor 13 is detected after plate body 1 goes to default angle,
There is halt instruction to controller 20, controller 20 stops motor 12 after receiving halt instruction.The predetermined angular is caused Lou
Fluid apertures 3 is in low level, is so more beneficial for waste liquid and discharges more quickly, does not remain, and reduces experiment risk, increased experiment accurate
Degree.The angle can be according to practical situation (such as：Bottom land is located at according to liquid-leaking nozzle 3 or Sidewall angles is different) in position sensor
Set in 13.
Wherein, gripper shoe 8 can be the gripper shoe with heating function (heating wire is arranged in Heat Conduction Material), with controller
20 connections, when some reactions need to carry out under higher than ambient temperature, while temperature constant temperature after the pre-treatment is made, can
Gripper shoe 8 is controlled by controller 20 to generate heat, makes reactant liquor constant in higher temperature.Refrigeration can also be included in the reaction platform
Device 14, is connected with controller 20, the connection gripper shoe 8 of refrigerating plant 14.Some reactions need to carry out at temperatures below ambient
When, and temperature constant temperature after the pre-treatment is made, refrigerating plant 14 can be controlled by controller 20 and be freezed, due to gripper shoe
8 are made up of Heat Conduction Material, and according to heat transfer principle, in can making reactive tank 2, reactant liquor keeps constant low temperature.Can be in plate body 1
On a temperature sensor is installed again, to realize precise control to reacting liquid temperature, specifically, temperature sensor connection control
Device 20, the reacting liquid temperature of real-time monitoring is sent to controller 20, controller 20 according to the reacting liquid temperature of real-time monitoring come
Control gripper shoe 8 is heated or refrigerating plant 14 freezes, so that reactant liquor keeps constant temperature.
Reaction platform also includes waste liquid pool 21,21 connected drainage pump 6 of waste liquid pool, for containing the waste liquid of the exclusion of reactive tank 2.
In the present embodiment, liquid-leaking nozzle 3 may be located at 2 bottom of reactive tank (as shown in Figure 3) or side wall (as shown in Figure 2),
During the wall of side, liquid-leaking nozzle is more beneficial for fluid removal near the position of bottom land.
In the present embodiment, also removably it is provided with plate body 1 for covering the cover plate 7 of reactive tank 2.Cover plate 7 can not be complete
Cover full reactive tank 2, leave small gap, in order to fluid injection in reactive tank 2, and avoided in reaction as far as possible dust or
Other impurities fall into reactive tank 2 causes reaction precision to be forbidden, while and can reduce reactant liquor (volatile reaction as far as possible
Liquid) the cross-contamination of differential responses groove 2 that brings of volatilization.Wherein, cover plate 7 can be one lid of a monoblock or a groove.
Plate body 1 is made using PP, PC, PE material.On nucleic acid molecular cross instrument, metal material is replaced using PP, PC, PE
Sptting plate, it is to avoid there is chemical reaction in metal itself and reactant, while such material is easy to do into medical disposable material, reduce
The pollution that Reusability brings.
Preferred embodiment of the present utility model is the foregoing is only, it is not to limit this utility model, all in this practicality
Within new spirit and principle, any modification, equivalent substitution and improvements made etc. should be included in guarantor of the present utility model
Within the scope of shield.
Claims (10)
1. a kind of nucleic acid molecular cross instrument, it is characterised in that include：Casing, reaction platform, injection needle, topping-up pump, fluid reservoir,
Temperature sensor, register and controller；The reaction platform and injection needle relatively movably can be arranged in the casing,
The topping-up pump connection injection needle and fluid reservoir, the pipeline that the topping-up pump is connected with injection needle are provided with the first electrically-controlled valve, institute
State the pipeline between topping-up pump and fluid reservoir and be provided with the second electrically-controlled valve, the temperature sensor is located in the fluid reservoir, institute
State register and connect the fluid reservoir, the controller connects the register, temperature sensor and topping-up pump.
2. nucleic acid molecular cross instrument as claimed in claim 1, it is characterised in that also include：Liquid level sensor, the liquid level are passed
Sensor is located in the fluid reservoir, and connects the controller.
3. nucleic acid molecular cross instrument as claimed in claim 1, it is characterised in that the reaction platform is fixed on bottom of shell,
Injection needle is arranged on above the reaction platform by three-dimensional moving mechanism, and the three-dimensional moving mechanism connects the controller.
4. nucleic acid molecular cross instrument as claimed in claim 1, it is characterised in that the reaction platform is pacified by moveable platform
The bottom of shell is mounted in, the moveable platform connects the controller, and the injection needle is fixed on above reaction platform.
5. nucleic acid molecular cross instrument as claimed in claim 1, it is characterised in that photographic head is additionally provided with the casing, it is described
Photographic head connects the controller.
6. nucleic acid molecular cross instrument as claimed in claim 1, it is characterised in that also include in the controller for wirelessly connecting
Connect the wireless communications chips of host computer.
7. the nucleic acid molecular cross instrument as any one of claim 1～6, it is characterised in that the reaction platform includes：
Sptting plate and the agitating device for waving the Sptting plate, the Sptting plate include：Positive displacement pump, plate body and it is formed in plate body
On some reactive tanks, be provided with liquid-leaking nozzle in each described reactive tank, each liquid-leaking nozzle is connected with leakage pipe, on each leakage pipe
3rd electrically-controlled valve, the leakage pipe connected drainage pump, the 3rd electrically-controlled valve and the positive displacement pump connection control are installed
Device, the agitating device connect the plate body.
8. nucleic acid molecular cross instrument as claimed in claim 7, it is characterised in that the agitating device includes：Gripper shoe,
Seat, rocking arm, shaft coupling, position sensor and motor, the plate body are located in gripper shoe, and one end of gripper shoe is rotatably coupled
Bearing, the other end are rotatably coupled the rocking arm, and rocking arm connects the motor by the shaft coupling, the motor and described
Position sensor connects the controller.
9. nucleic acid molecular cross instrument as claimed in claim 7, it is characterised in that the liquid-leaking nozzle is located at the reaction tank bottom
Or side wall.
10. nucleic acid molecular cross instrument as claimed in claim 7, it is characterised in that use is also removably provided with the plate body
In the cover plate for covering the reactive tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621073749.XU CN206109410U (en) | 2016-09-22 | 2016-09-22 | Nucleic acid molecule hybridizing apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621073749.XU CN206109410U (en) | 2016-09-22 | 2016-09-22 | Nucleic acid molecule hybridizing apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206109410U true CN206109410U (en) | 2017-04-19 |
Family
ID=58520655
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621073749.XU Expired - Fee Related CN206109410U (en) | 2016-09-22 | 2016-09-22 | Nucleic acid molecule hybridizing apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206109410U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106282007A (en) * | 2016-09-22 | 2017-01-04 | 周江 | Nucleic acid molecular cross instrument |
-
2016
- 2016-09-22 CN CN201621073749.XU patent/CN206109410U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106282007A (en) * | 2016-09-22 | 2017-01-04 | 周江 | Nucleic acid molecular cross instrument |
| CN106282007B (en) * | 2016-09-22 | 2019-06-14 | 周江 | Nucleic acid molecular cross instrument |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170419 Termination date: 20200922 |
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| CF01 | Termination of patent right due to non-payment of annual fee |