CN106190859A - A kind of Grifola frondosa culture medium based on oils and fats waste residue and Grifola frondosa liquid spawn preparation method - Google Patents

Abstract

The invention discloses a kind of Grifola frondosa culture medium based on oils and fats waste residue, including following parts by weight of component: oils and fats waste residue, Calx, alginic acid, beer yeast powder, nano-scaled electric stone powder, Nano bacteria cellulose 5 etc..The present invention uses major ingredient oils and fats waste residue, Calx, beer yeast powder etc. to prepare Grifola frondosa liquid spawn as culture medium, material source is extensive, achieve oils and fats waste residue and make use of popularization in Grifola frondosa strain cultivation, deficiency for oils and fats waste residue dissolubility difference, by Calx and alginic acid, oils and fats waste residue surface is carried out graft modification, add the stability of fluid medium；Simultaneously, culture medium adds nanometer bacteria cellulose, while providing nutrient raw material for Grifola frondosa, also improve the stability, uniformity of fluid medium, growth promoter for Grifola frondosa liquid spawn mycelium pellet provides good space, the mycelium pellet of Grifola frondosa liquid spawn prepared by the present invention is evenly distributed, and size is stable, and quality is good.

Description

Prepared by a kind of Grifola frondosa culture medium based on oils and fats waste residue and Grifola frondosa liquid spawn Method

Technical field

The present invention relates to liquid spawn technical field, particularly relate to a kind of Grifola frondosa culture medium based on oils and fats waste residue and Grifola frondosa liquid spawn preparation method.

Background technology

In Grifola frondosa production process, liquid spawn has the advantage that solid spawn is incomparable, and liquid spawn has life The product cycle is short, and cell age is short and consistent, and purity is high, energetic, inoculates simple and efficient, easily carries out batch production, scale, standard metaplasia The advantages such as product.Now, increasing people is research and use liquid spawn, and the popularization of liquid spawn and use are also real Existing edible fungus industrial, scale, standardized inexorable trend.But liquid spawn there is also defect and the deficiency of self: 1, the many fast growths of liquid spawn germination point, but penetration power is not strong, and compost is crossed thick mycelia and is difficult to have thorough grasp compost, mycelia Will be along compost surface fast-growth, the internal hyphae length of compost is little, the problem having had a strong impact on strain yield and quality； Although 2, conventional liquid spawn solves some problems that solid spawn exists, can substantially shorten cultivation period, strengthen strain Vigor and resistance, reduction strain pollution rate, strain quality regularity is improved, but still suffer from the liquid obtained that such as ferments The deficiencies such as in strain the physical characteristic such as the density of fungus ball, size, the uniformity is less desirable, if the method sheared with stirring will Fungus ball is smashed, and the strain uniformity obtained can be more preferable, but, high-speed stirred is pulverized and strain is injured pole by the shearing force of making beating Greatly, the quality impact on strain is overall is the most serious.Exploitation is evenly distributed and has the high-quality liquid of smaller particle mycelium pellet Body strain is significant.Publication number CN105309194A " edible fungi particle liquid strain production new technology " provides one Mycelium pellet particle diameter 0.5 edible fungi particle liquid strain, the increase edible fungi liquid strain culture fluid ventilation of employing, make Ventilation is suitable at 1:0.6 0.9V/V min, and in air blow tank, device agitator makes culture fluid eddy motion simultaneously Extending the technology operation method of the moving line of bubble, the method for this production particle liquid strain is to change from production technology Entering, liquid spawn culture medium aspect does not improves, and implements technical difficulty and requires height；Publication number CN105130516A " molecular level biological medium manufacture method " refer to molecular level biological medium nutritional labeling and can the most comprehensively be absorbed, Promote the growth of liquid spawn, but do not mention the molecular level biological medium shadow to liquid spawn mycelium pellet diameter Ring.Development is a kind of improves edible fungus species product based on liquid spawn culture medium composition of raw material improvement liquid spawn mycelium pellet diameter Amount and the technology of quality, have great importance.

Nano bacteria cellulose is one of abundant renewable product of nature, and Nano bacteria cellulose is cellulose Physics minimum structural unit, refers to the fiber between diameter 1-100nm, Nano bacteria cellulose light weight, and good biocompatibility can Degraded, renewable, reactivity is high, and it is high to have Young's modulus, and the degree of polymerization is high, and cleanliness factor is high, and intensity is high, and specific surface area is big Advantage.Nano bacteria cellulose is made as edible fungus liquid culture growth medium raw material, the mycelia to edible fungi liquid strain Ball distribution and diameter tool have a certain impact, and then play the effect improving edible fungus species quality.

Summary of the invention

The object of the invention is contemplated to make up the defect of prior art, it is provided that a kind of Grifola frondosa based on oils and fats waste residue is cultivated Base and Grifola frondosa liquid spawn preparation method.

The present invention is achieved by the following technical solutions:

A kind of Grifola frondosa culture medium based on oils and fats waste residue, including following parts by weight of component: oils and fats waste residue 23-25, Calx 3-4, Alginic acid 5-6, beer yeast powder 19-20, peptone 9-10, glucose 11-12, citrate of magnesia 2-2.2, Sodium Pyruvate 1.2- 1.3, potassium selenite 1.1-1.2, Triammonium citrate 1.3-1.4, vitaminB10 .3-0.4, heteroauxing 0.2-0.3, casein 0.3-0.4, fumaric acid 0.3-0.4, Magnesium sulfate heptahydrate 1.1-1.2, Gypsum Fibrosum 0.7-0.8, potassium dihydrogen phosphate 0.6-0.7, mistake Calcium phosphate 0.4-0.5, nano-scaled electric stone powder 5.8-6, Nano bacteria cellulose 6.4-6.5, appropriate water.

A kind of method using above-mentioned culture medium to prepare Grifola frondosa liquid spawn, comprises the following steps:

(1), half nano-scaled electric stone powder and water being mixed according to 0.1g/L, energising excites that to prepare sterilized water standby；By seven water Magnesium sulfate, Gypsum Fibrosum, potassium dihydrogen phosphate, the total amount of calcium superphosphate and sterilized water are blended in 30 DEG C of stirring and dissolving by 1g/L and obtain solution One；By peptone, glucose, citrate of magnesia, Sodium Pyruvate, potassium selenite, Triammonium citrate, vitamin B1, heteroauxing, do Casein, fumaric total amount and sterilized water dissolve to obtain solution two by 20g/L mix and blend；Oils and fats waste residue is put into superfine powder Pulverization process 30min in broken machine, the water little fire literary composition putting into addition gross weight 4 times in pot afterwards together with Calx, alginic acid boils 3h, Cool down afterwards, be placed again into micronizing 1h in super micron mill, obtain solvable superfine powder, by this solvable superfine powder, beer yeast The total amount of powder and sterilized water press 300g/L mix and blend, and carry out pasteurization enzyme denaturing 10min carrying out 98 DEG C, obtain solution three； By second half nano-tourmaline powder and the water mixing and stirring of Nano bacteria cellulose and gross weight 3-4 times and use high temperature Steam sterilization, obtains solution four standby；

(2), by solution three keep 20 DEG C, solution two keep 25 DEG C, solution one keep 30 DEG C, first solution one is joined solution two Middle mixing and stirring obtains complex liquid, obtains nutritional solution this complex liquid joins mixing and stirring in solution three, and adjusts PH, hereafter carries out energising and excites process 30min to obtain fluid medium standby this nutritional solution；

(3), by Grifola frondosa mother planting first to be inoculated in gnotobasis on the fluid medium of half and carry out liquid fermentation, ferment bar Part is pH5.4-5.5, inoculum concentration 14%, temperature 27-28 DEG C, shaking speed 190r/min, cultivate 24-25h, obtain Grifola frondosa liquid Strain one；

(4), by the residue fluid medium of half, the aseptic mix homogeneously of solution four, modification medicinal residues puffing powder Radix Ophiopogonis is added afterwards And the sterilized water of gross weight 2 times, after high pressure homogenize, energising intensifies cooperation high temperature sterilizing again, and is placed into aseptic culture In Ping, obtain complex medium, the Grifola frondosa liquid spawn one that step (3) obtains is connect by the inoculum concentration of 58% in gnotobasis Planting in this culture bottle, temperature 25-26 DEG C, shaking speed 190r/min also blasts aseptic oxygen, cultivates 39-40h and get final product.

The invention have the advantage that

The present invention uses major ingredient oils and fats waste residue, Calx, beer yeast powder, glucose, peptone to coordinate citrate of magnesia, acetone acid The nutrient substance such as sodium, potassium tellurite, Triammonium citrate, vitamin B1, heteroauxing, casein, fumaric acid are as cultivation Grifola frondosa liquid spawn prepared by base, and material source is extensive, it is achieved that oils and fats waste residue make use of Grifola frondosa strain cultivation Promote, for the deficiency of oils and fats waste residue dissolubility difference, by Calx and alginic acid, oils and fats waste residue surface has been carried out grafting and changed Property, add the stability of fluid medium；Meanwhile, culture medium adds nanometer bacteria cellulose, provide battalion for Grifola frondosa While supporting raw material, also improving the stability, uniformity of fluid medium, the growth for Grifola frondosa liquid spawn mycelium pellet is sent out Educate and provide good space, be difficult to have thorough grasp compost, only in training when overcoming traditional liquid-liquid strain mycelium pellet growth The epontic defect of nutriment and deficiency, the mycelium pellet of Grifola frondosa liquid spawn prepared by the present invention is evenly distributed, and size is stable, Quality is good.

Detailed description of the invention

A kind of Grifola frondosa culture medium based on oils and fats waste residue, including following parts by weight of component: oils and fats waste residue 23, Calx 3, sea Alginic acid 5, beer yeast powder 19, peptone 9, glucose 11, citrate of magnesia 2, Sodium Pyruvate 1.2, potassium selenite 1.1, citric acid Three ammoniums 1.3, vitaminB10 .3, heteroauxing 0.2, casein 0.3, fumaric acid 0.3, Magnesium sulfate heptahydrate 1.1, Gypsum Fibrosum 0.7, potassium dihydrogen phosphate 0.6, calcium superphosphate 0.4, nano-scaled electric stone powder 5.8, Nano bacteria cellulose 6.4, appropriate water.

A kind of method using above-mentioned culture medium to prepare Grifola frondosa liquid spawn, comprises the following steps:

(1), half nano-scaled electric stone powder and water being mixed according to 0.1g/L, energising excites that to prepare sterilized water standby；By seven water Magnesium sulfate, Gypsum Fibrosum, potassium dihydrogen phosphate, the total amount of calcium superphosphate and sterilized water are blended in 30 DEG C of stirring and dissolving by 1g/L and obtain solution One；By peptone, glucose, citrate of magnesia, Sodium Pyruvate, potassium selenite, Triammonium citrate, vitamin B1, heteroauxing, do Casein, fumaric total amount and sterilized water dissolve to obtain solution two by 20g/L mix and blend；Oils and fats waste residue is put into superfine powder Pulverization process 30min in broken machine, the water little fire literary composition putting into addition gross weight 4 times in pot afterwards together with Calx, alginic acid boils 3h, Cool down afterwards, be placed again into micronizing 1h in super micron mill, obtain solvable superfine powder, by this solvable superfine powder, beer yeast The total amount of powder and sterilized water press 300g/L mix and blend, and carry out pasteurization enzyme denaturing 10min carrying out 98 DEG C, obtain solution three； By second half nano-tourmaline powder and Nano bacteria cellulose and the water mixing and stirring of gross weight 3 times and use high temperature to steam Vapour sterilizing, obtains solution four standby；

(2), by solution three keep 20 DEG C, solution two keep 25 DEG C, solution one keep 30 DEG C, first solution one is joined solution two Middle mixing and stirring obtains complex liquid, obtains nutritional solution this complex liquid joins mixing and stirring in solution three, and adjusts PH, hereafter carries out energising and excites process 30min to obtain fluid medium standby this nutritional solution；

(3), by Grifola frondosa mother planting first to be inoculated in gnotobasis on the fluid medium of half and carry out liquid fermentation, ferment bar Part is pH5.4, inoculum concentration 14%, temperature 27 DEG C, shaking speed 190r/min, cultivate 24h, obtain Grifola frondosa liquid spawn one；

(4), by the residue fluid medium of half, the aseptic mix homogeneously of solution four, modification medicinal residues puffing powder Radix Ophiopogonis is added afterwards And the sterilized water of gross weight 2 times, after high pressure homogenize, energising intensifies cooperation high temperature sterilizing again, and is placed into aseptic culture In Ping, obtain complex medium, the Grifola frondosa liquid spawn one that step (3) obtains is connect by the inoculum concentration of 58% in gnotobasis Planting in this culture bottle, temperature 25 DEG C, shaking speed 190r/min also blasts aseptic oxygen, cultivates 39h and get final product.

Claims (2)

1. a Grifola frondosa culture medium based on oils and fats waste residue, it is characterised in that include following parts by weight of component: oils and fats waste residue 23-25, Calx 3-4, alginic acid 5-6, beer yeast powder 19-20, peptone 9-10, glucose 11-12, citrate of magnesia 2-2.2, Sodium Pyruvate 1.2-1.3, potassium selenite 1.1-1.2, Triammonium citrate 1.3-1.4, vitaminB10 .3-0.4, heteroauxing 0.2-0.3, casein 0.3-0.4, fumaric acid 0.3-0.4, Magnesium sulfate heptahydrate 1.1-1.2, Gypsum Fibrosum 0.7-0.8, di(2-ethylhexyl)phosphate Hydrogen potassium 0.6-0.7, calcium superphosphate 0.4-0.5, nano-scaled electric stone powder 5.8-6, Nano bacteria cellulose 6.4-6.5, appropriate Water.

2. one kind uses the method that the culture medium described in claim 1 prepares Grifola frondosa liquid spawn, it is characterised in that include with Lower step:

(1), half nano-scaled electric stone powder and water being mixed according to 0.1g/L, energising excites that to prepare sterilized water standby；By seven water Magnesium sulfate, Gypsum Fibrosum, potassium dihydrogen phosphate, the total amount of calcium superphosphate and sterilized water are blended in 30 DEG C of stirring and dissolving by 1g/L and obtain solution One；By peptone, glucose, citrate of magnesia, Sodium Pyruvate, potassium selenite, Triammonium citrate, vitamin B1, heteroauxing, do Casein, fumaric total amount and sterilized water dissolve to obtain solution two by 20g/L mix and blend；Oils and fats waste residue is put into superfine powder Pulverization process 30min in broken machine, the water little fire literary composition putting into addition gross weight 4 times in pot afterwards together with Calx, alginic acid boils 3h, Cool down afterwards, be placed again into micronizing 1h in super micron mill, obtain solvable superfine powder, by this solvable superfine powder, beer yeast The total amount of powder and sterilized water press 300g/L mix and blend, and carry out pasteurization enzyme denaturing 10min carrying out 98 DEG C, obtain solution three； By second half nano-tourmaline powder and the water mixing and stirring of Nano bacteria cellulose and gross weight 3-4 times and use high temperature Steam sterilization, obtains solution four standby；

(2), by solution three keep 20 DEG C, solution two keep 25 DEG C, solution one keep 30 DEG C, first solution one is joined solution two Middle mixing and stirring obtains complex liquid, obtains nutritional solution this complex liquid joins mixing and stirring in solution three, and adjusts PH, hereafter carries out energising and excites process 30min to obtain fluid medium standby this nutritional solution；

(3), by Grifola frondosa mother planting first to be inoculated in gnotobasis on the fluid medium of half and carry out liquid fermentation, ferment bar Part is pH5.4-5.5, inoculum concentration 14%, temperature 27-28 DEG C, shaking speed 190r/min, cultivate 24-25h, obtain Grifola frondosa liquid Strain one；

(4), by the residue fluid medium of half, the aseptic mix homogeneously of solution four, modification medicinal residues puffing powder Radix Ophiopogonis is added afterwards And the sterilized water of gross weight 2 times, after high pressure homogenize, energising intensifies cooperation high temperature sterilizing again, and is placed into aseptic culture In Ping, obtain complex medium, the Grifola frondosa liquid spawn one that step (3) obtains is connect by the inoculum concentration of 58% in gnotobasis Planting in this culture bottle, temperature 25-26 DEG C, shaking speed 190r/min also blasts aseptic oxygen, cultivates 39-40h and get final product.