CN106047725A - Lepista nuda culture medium based on yellow wine lees and preparation method of lepista nuda liquid strain - Google Patents
Lepista nuda culture medium based on yellow wine lees and preparation method of lepista nuda liquid strain Download PDFInfo
- Publication number
- CN106047725A CN106047725A CN201610552378.1A CN201610552378A CN106047725A CN 106047725 A CN106047725 A CN 106047725A CN 201610552378 A CN201610552378 A CN 201610552378A CN 106047725 A CN106047725 A CN 106047725A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- solution
- yellow wine
- wine lees
- lepista
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The invention discloses a lepista nuda culture medium based on yellow wine lees. The lepista nuda culture medium is prepared from, by weight, the yellow wine lees, mint leaves, onion extract, enzymatically-hydrolyzed fish scale powder, yeast extract, soy isoflavone, zinc lactate, adenine, nanoscale tourmaline powder, bacterial nano cellulose and the like. The culture medium is prepared from the yellow wine lees, the mint leaves, the enzymatically-hydrolyzed fish scale powder, molasses and the like and used for preparing a lepista nuda liquid strain, the adopted culture medium material is low in price cost, certain fragrance is provided for the culture medium through treatment of the raw materials, and a certain effect is achieved for improving the fragrance of lepista nuda; meanwhile, nutrient substances of the bacterial nano cellulose with the infectious microbe restraining effect are added into the culture medium, the nutrient substances provide good growth and development space for lepista nuda liquid strain mycelium pellets, along with degradation and absorption use of the bacterial nano cellulose, the onion extract with the infectious microbe restraining effect exerts effects, and a good environment is provided for lepista nuda fruiting bodies.
Description
Technical field
The present invention relates to liquid spawn technical field, particularly relate to a kind of Lepista mucla (Bull.:Fr.) Cooke culture medium based on yellow wine lees and
Lepista mucla (Bull.:Fr.) Cooke liquid spawn preparation method.
Background technology
In Lepista mucla (Bull.:Fr.) Cooke production process, liquid spawn has the advantage that solid spawn is incomparable, and liquid spawn has
With short production cycle, cell age is short and consistent, and purity is high, energetic, inoculates simple and efficient, easily carries out batch production, scale, standardization
The advantages such as production.Now, increasing people is research and use liquid spawn, and the popularization of liquid spawn and use are also
Realize edible fungus industrial, scale, standardized inexorable trend.But liquid spawn there is also the defect and not of self
Foot: 1, the many fast growths of liquid spawn germination point, but penetration power is not strong, and compost is crossed thick mycelia and is difficult to have thorough grasp compost,
Mycelia will be along compost surface fast-growth, and the internal hyphae length of compost is little, has had a strong impact on strain yield and quality
Problem；Although 2, conventional liquid spawn solves some problems that solid spawn exists, can substantially shorten cultivation period, enhancing
Spawn activity and resistance, reduction strain pollution rate, strain quality regularity is improved, but still suffer from such as fermenting and obtain
The deficiencies such as in liquid spawn the physical characteristic such as the density of fungus ball, size, the uniformity is less desirable, if the side sheared with stirring
Fungus ball is smashed by method, and the strain uniformity obtained can be more preferable, but, high-speed stirred is pulverized and strain is hindered by the shearing force of making beating
Greatly, the quality impact overall on strain is the most serious for evil.Exploitation is evenly distributed and has the high-quality of smaller particle mycelium pellet
Liquid spawn significant.Publication number CN105309194A " edible fungi particle liquid strain production new technology " provides
A kind of mycelium pellet particle diameter 0.5 edible fungi particle liquid strain, employing increase edible fungi liquid strain culture fluid ventilation
Amount, making ventilation is suitable at 1:0.6 0.9V/V min, and in air blow tank, device agitator makes culture fluid eddy current simultaneously
Motion extends the technology operation method of the moving line of bubble, and the method for this production particle liquid strain is enterprising from production technology
Row improves, and liquid spawn culture medium aspect does not improves, and implements technical difficulty and requires height；Publication number
Refer to molecular level biological medium nutritional labeling can be quick for CN105130516A " molecular level biological medium manufacture method "
Comprehensively absorbed, promote the growth of liquid spawn, but do not mention molecular level biological medium to liquid spawn mycelia
The impact of bulb diameter size.Development is a kind of to be changed based on liquid spawn culture medium composition of raw material improvement liquid spawn mycelium pellet diameter
Kind edible fungus species yield and the technology of quality, have great importance.
Nano bacteria cellulose is one of abundant renewable product of nature, and Nano bacteria cellulose is cellulose
Physics minimum structural unit, refers to the fiber between diameter 1-100nm, Nano bacteria cellulose light weight, and good biocompatibility can
Degraded, renewable, reactivity is high, and it is high to have Young's modulus, and the degree of polymerization is high, and cleanliness factor is high, and intensity is high, and specific surface area is big
Advantage.Nano bacteria cellulose is made as edible fungus liquid culture growth medium raw material, the mycelia to edible fungi liquid strain
Ball distribution and diameter tool have a certain impact, and then play the effect improving edible fungus species quality.
Summary of the invention
The object of the invention is contemplated to make up the defect of prior art, it is provided that a kind of Lepista mucla (Bull.:Fr.) Cooke based on yellow wine lees is cultivated
Base and Lepista mucla (Bull.:Fr.) Cooke liquid spawn preparation method.
The present invention is achieved by the following technical solutions:
A kind of Lepista mucla (Bull.:Fr.) Cooke culture medium based on yellow wine lees, including following parts by weight of component: yellow wine lees 140-145, mentha leave 40-
45, Bulbus Allii Cepae extract 3-4, enzymolysis pearl white 120-130, molasses 9-10, Nafusaku 1-1.2, yeast extract 4-5, Semen sojae atricolor
Isoflavone 1.5-2, zinc lactate 0.9-1, adenine 0.8-1, anti-crawl agentfoam oil 1-1.1, magnesium sulfate 1.2-1.3, Gypsum Fibrosum 0.6-0.7, phosphorus
Acid dihydride potassium 0.5-0.6, calcium superphosphate 0.7-0.8, nano-scaled electric stone powder 5.6-6, Nano bacteria cellulose 6.2-6.5, fibre
Dimension element enzyme 3-4, saccharifying enzyme 2.8-3, appropriate water.
A kind of method using above-mentioned culture medium to prepare Lepista mucla (Bull.:Fr.) Cooke liquid spawn, comprises the following steps:
(1), nano-scaled electric stone powder and water being mixed according to 0.1g/L, energising excites that to prepare sterilized water standby；By anti-crawl agentfoam oil, stone
Cream, magnesium sulfate, potassium dihydrogen phosphate, the total amount of calcium superphosphate and sterilized water dissolve to obtain solution one by 1g/L mix and blend；General, sugar
Honey, Nafusaku, yeast extract, soybean isoflavone, zinc lactate, the total amount of adenine and sterilized water are blended in 30 by 100g/L
DEG C stirring and dissolving obtains solution two；Pulverization process 30min in super micron mill, the least fire are put in yellow wine lees, mentha leave mixing
Bake 1h to carry out detoxification flavouring and process to obtain flavouring yellow wine lees micropowder, flavouring yellow wine lees micropowder will add cellulase, saccharifying enzyme
And the water of gross weight 4 times, stir enzymolysis processing 1h at 40 DEG C, filter afterwards, obtain filtrate and filtering residue, by filtrate, enzymolysis fish scale
The total amount of powder and sterilized water press 350g/L mixing and stirring, and carry out pasteurization enzyme denaturing 10min carrying out 98 DEG C, obtain solution
Three is standby；Filtering residue is put into ultra micro nano grinder carries out pulverization process 20min, use high temperature puffing machine to process afterwards
It is standby that 15min obtains ultra micro composite powder；
(2), by solution three keep 20 DEG C, solution two keep 25 DEG C, solution one keep 30 DEG C, first solution one is joined solution two
Middle mixing and stirring obtains complex liquid, obtains nutritional solution this complex liquid joins mixing and stirring in solution three, and adjusts
PH, hereafter carries out energising and excites process 30min to obtain fluid medium standby this nutritional solution；
(3), by Lepista mucla (Bull.:Fr.) Cooke mother plant first to be inoculated in gnotobasis on the fluid medium of half and carry out liquid fermentation, fermentation
Condition is pH6-6.2, inoculum concentration 11%, temperature 29-30 DEG C, shaking speed 190r/min, cultivate 72-74h, obtains Lepista mucla (Bull.:Fr.) Cooke female
Liquid spawn one；
(4), by after the sterilized water mixing and stirring of Nano bacteria cellulose, Bulbus Allii Cepae extract and gross weight 1 times, use
Spray drying technology carries out embedding treatment, and the Nano bacteria cellulose must with suppression miscellaneous bacteria effect is standby；By residue half
Fluid medium, there is the Nano bacteria cellulose mix homogeneously of suppression miscellaneous bacteria effect, add ultra micro composite powder and total afterwards
The sterilized water that weight is 2 times, after high pressure homogenize, energising intensifies cooperation high temperature sterilizing again, and is placed in sterile culture flask,
Complex medium, the Lepista mucla (Bull.:Fr.) Cooke mother liquid spawn one step (3) obtained is inoculated in gnotobasis by the inoculum concentration of 45%
In this culture bottle, temperature 27-28 DEG C, shaking speed 200r/min also blasts aseptic oxygen, cultivates 50-55h and get final product.
The invention have the advantage that
The present invention use yellow wine lees, mentha leave, enzymolysis pearl white, molasses coordinate nutrient substance yeast extract, soybean isoflavone,
Zinc lactate, adenine, magnesium sulfate, potassium dihydrogen phosphate etc. prepare Lepista mucla (Bull.:Fr.) Cooke liquid spawn for culture medium, the cultivation base material of employing
Material Costco Wholesale is low, by raw-material process, giving the fragrance that culture medium is certain, to improving the fragrance improving Lepista mucla (Bull.:Fr.) Cooke
Tool has certain effect；Meanwhile, culture medium adds the nutrient substance of the Nano bacteria cellulose with suppression miscellaneous bacteria effect,
This nutrient substance is not only Lepista mucla (Bull.:Fr.) Cooke liquid spawn mycelium pellet and provides good growth promoter space, along with bacteria nano is fine
The degraded and absorbed of dimension element utilizes, and the Bulbus Allii Cepae extract with suppression miscellaneous bacteria effect plays effect, provides for Lepista mucla (Bull.:Fr.) Cooke sporophore
Good environment, liquid spawn mycelium pellet prepared by the present invention is evenly distributed, have Herba Menthae and aroma, simultaneously size to fit,
Pulverize without external force and pull an oar and just can obtain the liquid spawn of homogenizing.
Detailed description of the invention
A kind of Lepista mucla (Bull.:Fr.) Cooke culture medium based on yellow wine lees, including following parts by weight of component: yellow wine lees 140, mentha leave 40,
Bulbus Allii Cepae extract 3, enzymolysis pearl white 120, molasses 9, Nafusaku 1, yeast extract 4, soybean isoflavone 1.5, zinc lactate
0.9, adenine 0.8, anti-crawl agentfoam oil 1, magnesium sulfate 1.2, Gypsum Fibrosum 0.6, potassium dihydrogen phosphate 0.5, calcium superphosphate 0.7, nano-scaled electric
Stone powder 5.6, Nano bacteria cellulose 6.2, cellulase 3, saccharifying enzyme 2.8, appropriate water.
A kind of method using above-mentioned culture medium to prepare Lepista mucla (Bull.:Fr.) Cooke liquid spawn, comprises the following steps:
(1), nano-scaled electric stone powder and water being mixed according to 0.1g/L, energising excites that to prepare sterilized water standby；By anti-crawl agentfoam oil, stone
Cream, magnesium sulfate, potassium dihydrogen phosphate, the total amount of calcium superphosphate and sterilized water dissolve to obtain solution one by 1g/L mix and blend；General, sugar
Honey, Nafusaku, yeast extract, soybean isoflavone, zinc lactate, the total amount of adenine and sterilized water are blended in 30 by 100g/L
DEG C stirring and dissolving obtains solution two；Pulverization process 30min in super micron mill, the least fire are put in yellow wine lees, mentha leave mixing
Bake 1h to carry out detoxification flavouring and process to obtain flavouring yellow wine lees micropowder, flavouring yellow wine lees micropowder will add cellulase, saccharifying enzyme
And the water of gross weight 4 times, stir enzymolysis processing 1h at 40 DEG C, filter afterwards, obtain filtrate and filtering residue, by filtrate, enzymolysis fish scale
The total amount of powder and sterilized water press 350g/L mixing and stirring, and carry out pasteurization enzyme denaturing 10min carrying out 98 DEG C, obtain solution
Three is standby；Filtering residue is put into ultra micro nano grinder carries out pulverization process 20min, use high temperature puffing machine to process afterwards
It is standby that 15min obtains ultra micro composite powder；
(2), by solution three keep 20 DEG C, solution two keep 25 DEG C, solution one keep 30 DEG C, first solution one is joined solution two
Middle mixing and stirring obtains complex liquid, obtains nutritional solution this complex liquid joins mixing and stirring in solution three, and adjusts
PH, hereafter carries out energising and excites process 30min to obtain fluid medium standby this nutritional solution；
(3), by Lepista mucla (Bull.:Fr.) Cooke mother plant first to be inoculated in gnotobasis on the fluid medium of half and carry out liquid fermentation, fermentation
Condition is pH6, inoculum concentration 11%, temperature 29 DEG C, shaking speed 190r/min, cultivate 72h, obtain Lepista mucla (Bull.:Fr.) Cooke mother liquid spawn one；
(4), by after the sterilized water mixing and stirring of Nano bacteria cellulose, Bulbus Allii Cepae extract and gross weight 1 times, use
Spray drying technology carries out embedding treatment, and the Nano bacteria cellulose must with suppression miscellaneous bacteria effect is standby；By residue half
Fluid medium, there is the Nano bacteria cellulose mix homogeneously of suppression miscellaneous bacteria effect, add ultra micro composite powder and total afterwards
The sterilized water that weight is 2 times, after high pressure homogenize, energising intensifies cooperation high temperature sterilizing again, and is placed in sterile culture flask,
Complex medium, the Lepista mucla (Bull.:Fr.) Cooke mother liquid spawn one step (3) obtained is inoculated in gnotobasis by the inoculum concentration of 45%
In this culture bottle, temperature 27 DEG C, shaking speed 200r/min also blasts aseptic oxygen, cultivates 50h and get final product.
Claims (2)
1. a Lepista mucla (Bull.:Fr.) Cooke culture medium based on yellow wine lees, it is characterised in that include following parts by weight of component: yellow wine lees 140-
145, mentha leave 40-45, Bulbus Allii Cepae extract 3-4, enzymolysis pearl white 120-130, molasses 9-10, Nafusaku 1-1.2, yeast are taken out
Extract 4-5, soybean isoflavone 1.5-2, zinc lactate 0.9-1, adenine 0.8-1, anti-crawl agentfoam oil 1-1.1, magnesium sulfate 1.2-1.3, stone
Cream 0.6-0.7, potassium dihydrogen phosphate 0.5-0.6, calcium superphosphate 0.7-0.8, nano-scaled electric stone powder 5.6-6, Nano bacteria
Element 6.2-6.5, cellulase 3-4, saccharifying enzyme 2.8-3, appropriate water.
2. one kind uses the method that the culture medium described in claim 1 prepares Lepista mucla (Bull.:Fr.) Cooke liquid spawn, it is characterised in that include
Following steps:
(1), nano-scaled electric stone powder and water being mixed according to 0.1g/L, energising excites that to prepare sterilized water standby；By anti-crawl agentfoam oil, stone
Cream, magnesium sulfate, potassium dihydrogen phosphate, the total amount of calcium superphosphate and sterilized water dissolve to obtain solution one by 1g/L mix and blend；General, sugar
Honey, Nafusaku, yeast extract, soybean isoflavone, zinc lactate, the total amount of adenine and sterilized water are blended in 30 by 100g/L
DEG C stirring and dissolving obtains solution two；Pulverization process 30min in super micron mill, the least fire are put in yellow wine lees, mentha leave mixing
Bake 1h to carry out detoxification flavouring and process to obtain flavouring yellow wine lees micropowder, flavouring yellow wine lees micropowder will add cellulase, saccharifying enzyme
And the water of gross weight 4 times, stir enzymolysis processing 1h at 40 DEG C, filter afterwards, obtain filtrate and filtering residue, by filtrate, enzymolysis fish scale
The total amount of powder and sterilized water press 350g/L mixing and stirring, and carry out pasteurization enzyme denaturing 10min carrying out 98 DEG C, obtain solution
Three is standby；Filtering residue is put into ultra micro nano grinder carries out pulverization process 20min, use high temperature puffing machine to process afterwards
It is standby that 15min obtains ultra micro composite powder；
(2), by solution three keep 20 DEG C, solution two keep 25 DEG C, solution one keep 30 DEG C, first solution one is joined solution two
Middle mixing and stirring obtains complex liquid, obtains nutritional solution this complex liquid joins mixing and stirring in solution three, and adjusts
PH, hereafter carries out energising and excites process 30min to obtain fluid medium standby this nutritional solution；
(3), by Lepista mucla (Bull.:Fr.) Cooke mother plant first to be inoculated in gnotobasis on the fluid medium of half and carry out liquid fermentation, fermentation
Condition is pH6-6.2, inoculum concentration 11%, temperature 29-30 DEG C, shaking speed 190r/min, cultivate 72-74h, obtains Lepista mucla (Bull.:Fr.) Cooke female
Liquid spawn one；
(4), by after the sterilized water mixing and stirring of Nano bacteria cellulose, Bulbus Allii Cepae extract and gross weight 1 times, use
Spray drying technology carries out embedding treatment, and the Nano bacteria cellulose must with suppression miscellaneous bacteria effect is standby；By residue half
Fluid medium, there is the Nano bacteria cellulose mix homogeneously of suppression miscellaneous bacteria effect, add ultra micro composite powder and total afterwards
The sterilized water that weight is 2 times, after high pressure homogenize, energising intensifies cooperation high temperature sterilizing again, and is placed in sterile culture flask,
Complex medium, the Lepista mucla (Bull.:Fr.) Cooke mother liquid spawn one step (3) obtained is inoculated in gnotobasis by the inoculum concentration of 45%
In this culture bottle, temperature 27-28 DEG C, shaking speed 200r/min also blasts aseptic oxygen, cultivates 50-55h and get final product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610552378.1A CN106047725A (en) | 2016-07-14 | 2016-07-14 | Lepista nuda culture medium based on yellow wine lees and preparation method of lepista nuda liquid strain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610552378.1A CN106047725A (en) | 2016-07-14 | 2016-07-14 | Lepista nuda culture medium based on yellow wine lees and preparation method of lepista nuda liquid strain |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106047725A true CN106047725A (en) | 2016-10-26 |
Family
ID=57186454
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610552378.1A Pending CN106047725A (en) | 2016-07-14 | 2016-07-14 | Lepista nuda culture medium based on yellow wine lees and preparation method of lepista nuda liquid strain |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106047725A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108174744A (en) * | 2018-02-24 | 2018-06-19 | 刘峰 | Wild Lepista mucla (Bull.:Fr.) Cooke domesticating cultivation method |
| CN108770593A (en) * | 2018-05-16 | 2018-11-09 | 中国科学院微生物研究所 | A kind of Lepista mucla (Bull.:Fr.) Cooke bacterial strain and its sporocarp culture method |
| CN110679389A (en) * | 2019-11-18 | 2020-01-14 | 仁怀酱香白酒科研所 | Lepista nuda planting method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007135565A (en) * | 2005-11-22 | 2007-06-07 | Saitama Prefecture | New strain belonging to lepista sordida and artificial culture method |
| CN101699969A (en) * | 2009-11-05 | 2010-05-05 | 张纪明 | Submerged fermentation culture method of straw mushroom liquid strain and culture medium thereof |
| CN103416222A (en) * | 2013-07-31 | 2013-12-04 | 常州大学 | Lepista nuda spore cultivation and ripening method through hypha shake flasks and liquid submerged fermentation process |
| CN104620852A (en) * | 2015-01-20 | 2015-05-20 | 浙江大学 | Mushroom liquefied strain cultivation method |
| CN105112299A (en) * | 2015-08-27 | 2015-12-02 | 马鞍山市安康菌业有限公司 | Anion powder and shaggy cap high-biological-value conversion culture medium and preparation method thereof |
-
2016
- 2016-07-14 CN CN201610552378.1A patent/CN106047725A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007135565A (en) * | 2005-11-22 | 2007-06-07 | Saitama Prefecture | New strain belonging to lepista sordida and artificial culture method |
| CN101699969A (en) * | 2009-11-05 | 2010-05-05 | 张纪明 | Submerged fermentation culture method of straw mushroom liquid strain and culture medium thereof |
| CN103416222A (en) * | 2013-07-31 | 2013-12-04 | 常州大学 | Lepista nuda spore cultivation and ripening method through hypha shake flasks and liquid submerged fermentation process |
| CN104620852A (en) * | 2015-01-20 | 2015-05-20 | 浙江大学 | Mushroom liquefied strain cultivation method |
| CN105112299A (en) * | 2015-08-27 | 2015-12-02 | 马鞍山市安康菌业有限公司 | Anion powder and shaggy cap high-biological-value conversion culture medium and preparation method thereof |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108174744A (en) * | 2018-02-24 | 2018-06-19 | 刘峰 | Wild Lepista mucla (Bull.:Fr.) Cooke domesticating cultivation method |
| CN108770593A (en) * | 2018-05-16 | 2018-11-09 | 中国科学院微生物研究所 | A kind of Lepista mucla (Bull.:Fr.) Cooke bacterial strain and its sporocarp culture method |
| CN108770593B (en) * | 2018-05-16 | 2020-04-10 | 中国科学院微生物研究所 | Lepista nuda strain and fruiting body cultivation method thereof |
| CN110679389A (en) * | 2019-11-18 | 2020-01-14 | 仁怀酱香白酒科研所 | Lepista nuda planting method |
| CN110679389B (en) * | 2019-11-18 | 2021-10-26 | 仁怀酱香白酒科研所 | Lepista nuda planting method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105967919A (en) | Potato organic fertilizer | |
| CN103332971B (en) | A kind of Lemon enzyme liquid state fertilizer and preparation method thereof | |
| CN102850095A (en) | Preparation method by using Chinese medicine residue as cultivation material of edible mushroom pleurotus ostreatus | |
| CN106085876A (en) | A kind of straw mushroom liquid strain culture medium based on starch saccharification liquid and straw mushroom liquid strain preparation method | |
| CN106581083A (en) | Extraction method for ganoderma lucidum components, biological feed and preparation method thereof | |
| CN110342991A (en) | From the organic fertilizer of fermentation cordyceps sinensis | |
| CN103082145A (en) | Method for producing grape skin residue pig feed by utilizing lentinula edodes and yeast for symbiotic fermentation | |
| CN103460994B (en) | Cordyceps militaris nanometer selenium and preparation method thereof | |
| CN103570388A (en) | Production method of biological organic fertilizer | |
| CN107022493A (en) | A kind of aspergillus oryzae strain of high yield complex enzyme for feed and its application | |
| CN106047725A (en) | Lepista nuda culture medium based on yellow wine lees and preparation method of lepista nuda liquid strain | |
| CN107586200A (en) | The preparation method of one plant nutrient liquor | |
| CN106064982A (en) | Improve dedicated fertilizer of Citrus sinensis Osbeck diseases and insect pests resistance and yield and preparation method thereof | |
| CN106222093A (en) | Prepare the new method of flammulina velutipes liquid strains | |
| CN105660169A (en) | Method for cultivating Auricularia fuscosuccinea through Lyohyllum waste | |
| CN106085882A (en) | A kind of Armillaria mellea culture medium based on oil tea branch powder and halimasch liquid bacterial strains preparation method | |
| CN105085023A (en) | Foliar fertilizer prepared through Chinese medicine residue fermentation and preparation method thereof | |
| CN106366359A (en) | Pollution-free polymer preparing method based on bio-enzyme | |
| CN107058119A (en) | A kind of method for improving Cordyceps militaris liquid state fermentation production cordycepin and thermostable protein production of enzyme | |
| CN106011044A (en) | Method for preparing pleurotus citrinopileatus liquid strain through pleurotus citrinopileatus culture medium based on eupatorium adenophorum | |
| CN106047718A (en) | Cockroach-powder-based Ramaria botrytoides liquid strain culture medium and Ramaria botrytoides liquid strain preparation method | |
| CN106119123A (en) | A kind of white beech mushroom liquid spawn culture medium based on bean curd yellow pulp water and white beech mushroom liquid spawn preparation method | |
| CN105110899A (en) | Humic acid foliar fertilizer and preparation method therefor | |
| CN108383652A (en) | Agricultural wastes cultivate rich selenium germanium green organic element nutrition fertilizer | |
| CN107129390A (en) | Using Chinese medicine slag as organic fertilizer of raw material and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161026 |
|
| RJ01 | Rejection of invention patent application after publication |