CN106189352B - A kind of extracting method of carthamin yellow - Google Patents
A kind of extracting method of carthamin yellow Download PDFInfo
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- CN106189352B CN106189352B CN201610570659.XA CN201610570659A CN106189352B CN 106189352 B CN106189352 B CN 106189352B CN 201610570659 A CN201610570659 A CN 201610570659A CN 106189352 B CN106189352 B CN 106189352B
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- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
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Abstract
The invention discloses a kind of extracting method of carthamin yellow, including：Step 1：Remove the impurity in safflower raw material；Step 2：It is dry；Step 3：Sterilization；Step 4：The water of 5~16 times of safflower material qualities is added in safflower raw material after sterilization, added in first time separation of solid and liquid, then solid after first time separation of solid and liquid the water of 2~5 times of solids, carries out second of separation of solid and liquid, obtain Flos Carthami extract；Step 5：Ethyl alcohol is added in Flos Carthami extract and carries out first time elution, obtains the first eluent；Step 6：First eluent is subjected to pH value adjustment using glacial acetic acid；Step 7：Polyamide is added in the first eluent to be adsorbed；Step 8：Ethyl alcohol is added in the first eluent after absorption and carries out second of elution, obtains the second eluent；Step 9：It is concentrated after second eluent is adjusted to neutrality, obtains carthamin yellow.The present invention improves the content of carthamin yellow in extract.
Description
Technical field
The present invention relates to pharmaceutical technology field, more particularly to a kind of extracting method of carthamin yellow.
Background technology
Safflower is the important Chinese herbal medicine of China, is the dry flower of feverfew, includes safflower red pigment and carthamin yellow
Two kinds of pigments, warm-natured, acrid flavour have invigorate blood circulation, stasis-dispelling and pain-killing, reduce cholesterol, blood pressure lowering and other effects, be mainly used for the moon
Through uncomfortable, dysmenorrhoea, Amenorrhea, traumatic injury, also there is the effect of certain to diseases such as coronary heart disease, catarrhal jaundices.Carthamin yellow
It is the natural yellow pigment extracted from the petal of safflower, is Chalcone Compounds.Research finds that carthamin yellow is not only
A kind of of great value natural food colour has beautiful in colour, high temperature resistant, high pressure resistant, low temperature resistant, fast light, acidproof, resistance to reduction
And the advantages that antimicrobial, and also coronary artery dilator, anti-oxidant, protection cardiac muscle, blood pressure lowering, immunosupress and brain protection
Etc. a variety of pharmacological functions.In the prior art, from safflower extract carthamin yellow technological process generally include extracting and developing,
Purifying and preparation, but it is relatively low using the content of carthamin yellow that the method obtains.
Invention content
In view of this, the present invention provides a kind of extracting method of carthamin yellow, and which raises safflower yellow in extract
The content of element.
The present invention is solved the above problems by following technological means：
A kind of extracting method of carthamin yellow of the present invention, includes the following steps：Step 1：It removes in safflower raw material
Impurity；Step 2：Safflower raw material after removal impurity is placed in stove and is dried；Step 3：By dried safflower raw material
It is placed in sterilization container and is sterilized；Step 4：5~16 times of safflower material qualities of addition in safflower raw material after sterilization
Water stirs and carries out adding in 2~5 times of solids after 1~2h in first time separation of solid and liquid, then solid after first time separation of solid and liquid
Water carries out second of separation of solid and liquid after stirring 2~3h, the liquid and second of solid-liquid point for finally obtaining first time separation of solid and liquid
It is merged from obtained liquid, obtains Flos Carthami extract；Step 5：A concentration of 60%~80% is added in Flos Carthami extract
Ethyl alcohol carry out first time elution, obtain the first eluent；Step 6：By first eluent using a concentration of 0.7~
0.9% glacial acetic acid carries out pH value adjustment so that the pH value of the first eluent is 2~5；Step 7：In first that pH value is 2~5
The polyamide of preset ratio is added in eluent, is adsorbed in polyamide column；Step 8：The first eluent after absorption
The ethyl alcohol of middle addition a concentration of 60%~80% carries out second and elutes, and obtains the second eluent；Step 9：By the second eluent
It is concentrated after being adjusted to neutrality, obtains carthamin yellow.
Further, the safflower raw material after impurity that will remove in the step 2 is placed on to be dried in stove and specifically includes：
Safflower raw material after removal impurity is placed in the stove that temperature is 45 DEG C and is dried.
Further, dried safflower raw material is placed in sterilization container in the step 3 carries out sterilizing specific packet
It includes：Dried safflower raw material is placed in ultraviolet sterilization container and is sterilized.
Further, it is described second eluent is adjusted to neutrality after concentrate, after obtaining carthamin yellow, also wrap
It includes：Step 10：Obtained carthamin yellow is dried, and dried carthamin yellow is placed in storage tank and is deposited
Storage.
A kind of extracting method of carthamin yellow of the present invention has the advantages that：
The present invention provides a kind of extracting method of carthamin yellow, after impurity, drying and sterilization being removed successively
Safflower raw material add in water after, carry out first time separation of solid and liquid and second of separation of solid and liquid, finally obtained after separation of solid and liquid twice
Liquid merge, obtain Flos Carthami extract, certain density ethyl alcohol then added in Flos Carthami extract and is carried out for the first time
Elution, obtains the first eluent, and use certain density glacial acetic acid by the pH value adjustment of the first eluent for 2~5, then is gathering
It is adsorbed in amide column, certain density ethyl alcohol is finally added in the first eluent after absorption and carries out second of elution,
The second eluent is obtained, is concentrated after then the second eluent is adjusted to neutrality, finally obtains carthamin yellow, in use
The extracting method for stating carthamin yellow can obtain the higher carthamin yellow of content, that is, improve carthamin yellow in extract
Content.
Specific embodiment
A kind of extracting method of carthamin yellow of the present invention, mainly includes the following steps that：
Step 1：Remove the impurity in safflower raw material.
Step 2：Safflower raw material after removal impurity is placed in stove and is dried.
It is dried specifically, the safflower raw material after removal impurity is placed in the stove that temperature is 45 DEG C.
Step 3：Dried safflower raw material is placed in sterilization container and is sterilized.
It is sterilized specifically, dried safflower raw material is placed in ultraviolet sterilization container.
Step 4：The water of 5~16 times of safflower material qualities is added in safflower raw material after sterilization, is carried out after stirring 1~2h
The water of 2~5 times of solids is added in first time separation of solid and liquid, then solid after first time separation of solid and liquid, is carried out after stirring 2~3h
The liquid that second of separation of solid and liquid, the liquid for finally obtaining first time separation of solid and liquid and second of separation of solid and liquid obtain closes
And obtain Flos Carthami extract.
Step 5：The ethyl alcohol that a concentration of 60%~80% is added in Flos Carthami extract carries out first time elution, obtains first
Eluent.
Step 6：First eluent is subjected to pH value adjustment using a concentration of 0.7~0.9% glacial acetic acid so that the
The pH value of one eluent is 2~5.
Step 7：The polyamide of preset ratio is added in the first eluent for being 2~5 in pH value, is carried out in polyamide column
Absorption.
Step 8：The ethyl alcohol that a concentration of 60%~80% is added in the first eluent after absorption carries out second of elution,
Obtain the second eluent.
Step 9：It is concentrated after second eluent is adjusted to neutrality, obtains carthamin yellow.
Step 10：Obtained carthamin yellow is dried, and dried carthamin yellow is placed in storage tank
It is stored.
The present invention provides a kind of extracting method of carthamin yellow, after impurity, drying and sterilization being removed successively
Safflower raw material add in water after, carry out first time separation of solid and liquid and second of separation of solid and liquid, finally obtained after separation of solid and liquid twice
Liquid merge, obtain Flos Carthami extract, certain density ethyl alcohol then added in Flos Carthami extract and is carried out for the first time
Elution, obtains the first eluent, and use certain density glacial acetic acid by the pH value adjustment of the first eluent for 2~5, then is gathering
It is adsorbed in amide column, certain density ethyl alcohol is finally added in the first eluent after absorption and carries out second of elution,
The second eluent is obtained, is concentrated after then the second eluent is adjusted to neutrality, finally obtains carthamin yellow, in use
The extracting method for stating carthamin yellow can obtain the higher carthamin yellow of content, that is, improve carthamin yellow in extract
Content.
Embodiment one
Step 1：Remove the impurity in safflower raw material.
Step 2：Safflower raw material after removal impurity is placed in the stove that temperature is 45 DEG C and is dried.
Step 3：Dried safflower raw material is placed in ultraviolet sterilization container and is sterilized.
Step 4：The water of 5 times of safflower material qualities is added in safflower raw material after sterilization, is carried out for the first time after stirring 1h
The water of 2 times of solids is added in separation of solid and liquid, then solid after first time separation of solid and liquid, second of solid-liquid point is carried out after stirring 2h
From the liquid that the liquid and second of separation of solid and liquid for finally obtaining first time separation of solid and liquid obtain merges, and obtains safflower
Extracting solution.
Step 5：The ethyl alcohol that a concentration of 60% is added in Flos Carthami extract carries out first time elution, obtains the first elution
Liquid.
Step 6：First eluent is subjected to pH value adjustment using a concentration of 0.7% glacial acetic acid so that first washes
The pH value of de- liquid is 2.
Step 7：The polyamide of preset ratio is added in the first eluent for being 2 in pH value, is inhaled in polyamide column
It is attached.
Step 8：A concentration of 60% ethyl alcohol is added in the first eluent after absorption to carry out second and elute, and obtains the
Two eluents.
Step 9：It is concentrated after second eluent is adjusted to neutrality, obtains carthamin yellow.
Step 10：Obtained carthamin yellow is dried, and dried carthamin yellow is placed in storage tank
It is stored.
Obtained carthamin yellow is detected, testing result is as follows:
The content of carthamin yellow is 87% in the extract obtained by embodiment one.
Embodiment two
Step 1：Remove the impurity in safflower raw material.
Step 2：Safflower raw material after removal impurity is placed in the stove that temperature is 45 DEG C and is dried.
Step 3：Dried safflower raw material is placed in ultraviolet sterilization container and is sterilized.
Step 4：The water of 10 times of safflower material qualities is added in safflower raw material after sterilization, first is carried out after stirring 1.5h
The water of 3 times of solids is added in secondary separation of solid and liquid, then solid after first time separation of solid and liquid, stir carry out after 2.5h second it is solid
Liquid detaches, and the liquid that the liquid and second of separation of solid and liquid for finally obtaining first time separation of solid and liquid obtain is merged, obtained
Flos Carthami extract.
Step 5：The ethyl alcohol that a concentration of 70% is added in Flos Carthami extract carries out first time elution, obtains the first elution
Liquid；
Step 6：First eluent is subjected to pH value adjustment using a concentration of 0.8% glacial acetic acid so that first washes
The pH value of de- liquid is 4.
Step 7：The polyamide of preset ratio is added in the first eluent for being 4 in pH value, is inhaled in polyamide column
It is attached.
Step 8：A concentration of 70% ethyl alcohol is added in the first eluent after absorption to carry out second and elute, and obtains the
Two eluents.
Step 9：It is concentrated after second eluent is adjusted to neutrality, obtains carthamin yellow.
Step 10：Obtained carthamin yellow is dried, and dried carthamin yellow is placed in storage tank
It is stored.
Obtained carthamin yellow is detected, testing result is as follows:
The content of carthamin yellow is 94% in the extract obtained by embodiment two.
Finally illustrate, the above embodiments are merely illustrative of the technical solutions of the present invention and it is unrestricted, although with reference to compared with
The present invention is described in detail in good embodiment, it will be understood by those of ordinary skill in the art that, it can be to the skill of the present invention
Art scheme is modified or replaced equivalently, and without departing from the objective and range of technical solution of the present invention, should all be covered at this
In the right of invention.
Claims (4)
1. a kind of extracting method of carthamin yellow, it is characterised in that：Include the following steps：
Step 1：Remove the impurity in safflower raw material；
Step 2：Safflower raw material after removal impurity is placed in stove and is dried；
Step 3：Dried safflower raw material is placed in sterilization container and is sterilized；
Step 4：The water of 5~16 times of safflower material qualities is added in safflower raw material after sterilization, first is carried out after stirring 1~2h
The water of 2~5 times of solids is added in secondary separation of solid and liquid, then solid after first time separation of solid and liquid, second is carried out after stirring 2~3h
The liquid that secondary separation of solid and liquid, the liquid for finally obtaining first time separation of solid and liquid and second of separation of solid and liquid obtain merges,
Obtain Flos Carthami extract；
Step 5：The ethyl alcohol that a concentration of 60%~80% is added in Flos Carthami extract carries out first time elution, obtains the first elution
Liquid；
Step 6：First eluent is carried out p H values using a concentration of 0.7~0.9% glacial acetic acid to adjust so that first
The pH values of eluent are 2~5；
Step 7：The polyamide of preset ratio is added in the first eluent for being 2~5 in p H values, is inhaled in polyamide column
It is attached；
Step 8：The ethyl alcohol that a concentration of 60%~80% is added in the first eluent after absorption carries out second of elution, obtains
Second eluent；
Step 9：It is concentrated after second eluent is adjusted to neutrality, obtains carthamin yellow.
2. a kind of extracting method of carthamin yellow according to claim 1, it is characterised in that：General in the step 2
Safflower raw material after removal impurity, which is placed on to be dried in stove, to be specifically included：
Safflower raw material after removal impurity is placed in the stove that temperature is 45 DEG C and is dried.
3. a kind of extracting method of carthamin yellow according to claim 2, it is characterised in that：General in the step 3
Dried safflower raw material is placed in sterilization container sterilize and specifically include：
Dried safflower raw material is placed in ultraviolet sterilization container and is sterilized.
4. a kind of extracting method of carthamin yellow according to claim 1, it is characterised in that：Described by the second elution
Liquid is concentrated after being adjusted to neutrality, and after obtaining carthamin yellow, is further included：
Step 10：Obtained carthamin yellow is dried, and dried carthamin yellow is placed in storage tank and is carried out
Storage.
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1264708A (en) * | 1998-11-24 | 2000-08-30 | 北京市心肺血管疾病研究所 | Total safflower yellow and its preparing process and application |
| CN1272500A (en) * | 1998-11-24 | 2000-11-08 | 北京市心肺血管疾病研究所 | Safflower total uranidin, its preparation and application |
| CN1368503A (en) * | 2000-10-30 | 2002-09-11 | 山东绿叶制药股份有限公司 | Safflower lyochrome rich in carthamin A, its preparing process, its preparation and its medical usage |
| CN1422616A (en) * | 2001-12-06 | 2003-06-11 | 长春三真实业有限公司 | Use of hydroxy saflor yellow pigment A in preparing medicine for treating and preventing cardiovascular and cerebrovascular disease |
| CN1429830A (en) * | 2001-12-29 | 2003-07-16 | 山东绿叶制药股份有限公司 | Water-soluble safflower extract and its oral preparation for treating and preventing angiocardiopathy andcerebrova scular disease |
| CN1475272A (en) * | 2002-08-17 | 2004-02-18 | 李盛学 | Sydroxy carthamin A and its preparation method and use |
| CN1785988A (en) * | 2004-12-06 | 2006-06-14 | 山东绿叶天然药物研究开发有限公司 | Saflor yellow pigment rich in saflor yellow pigment B, its preparation method and use |
| CN102657691A (en) * | 2012-06-02 | 2012-09-12 | 悦康药业集团安徽天然制药有限公司 | Extraction process of carthamin yellow |
| CN103479702A (en) * | 2012-06-14 | 2014-01-01 | 北京星昊医药股份有限公司 | Extraction method and application of safflower yellow pigment |
| CN103665930A (en) * | 2013-12-31 | 2014-03-26 | 黔南民族师范学院 | Method of extracting natural edible pigment |
-
2016
- 2016-07-19 CN CN201610570659.XA patent/CN106189352B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1264708A (en) * | 1998-11-24 | 2000-08-30 | 北京市心肺血管疾病研究所 | Total safflower yellow and its preparing process and application |
| CN1272500A (en) * | 1998-11-24 | 2000-11-08 | 北京市心肺血管疾病研究所 | Safflower total uranidin, its preparation and application |
| CN1368503A (en) * | 2000-10-30 | 2002-09-11 | 山东绿叶制药股份有限公司 | Safflower lyochrome rich in carthamin A, its preparing process, its preparation and its medical usage |
| CN1422616A (en) * | 2001-12-06 | 2003-06-11 | 长春三真实业有限公司 | Use of hydroxy saflor yellow pigment A in preparing medicine for treating and preventing cardiovascular and cerebrovascular disease |
| CN1429830A (en) * | 2001-12-29 | 2003-07-16 | 山东绿叶制药股份有限公司 | Water-soluble safflower extract and its oral preparation for treating and preventing angiocardiopathy andcerebrova scular disease |
| CN1475272A (en) * | 2002-08-17 | 2004-02-18 | 李盛学 | Sydroxy carthamin A and its preparation method and use |
| CN1785988A (en) * | 2004-12-06 | 2006-06-14 | 山东绿叶天然药物研究开发有限公司 | Saflor yellow pigment rich in saflor yellow pigment B, its preparation method and use |
| CN102657691A (en) * | 2012-06-02 | 2012-09-12 | 悦康药业集团安徽天然制药有限公司 | Extraction process of carthamin yellow |
| CN103479702A (en) * | 2012-06-14 | 2014-01-01 | 北京星昊医药股份有限公司 | Extraction method and application of safflower yellow pigment |
| CN103665930A (en) * | 2013-12-31 | 2014-03-26 | 黔南民族师范学院 | Method of extracting natural edible pigment |
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