CN109517408A - A kind of acquisition methods of haematochrome - Google Patents
A kind of acquisition methods of haematochrome Download PDFInfo
- Publication number
- CN109517408A CN109517408A CN201710839072.9A CN201710839072A CN109517408A CN 109517408 A CN109517408 A CN 109517408A CN 201710839072 A CN201710839072 A CN 201710839072A CN 109517408 A CN109517408 A CN 109517408A
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- China
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- eluent
- solid
- raw material
- haematochrome
- safflower
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0096—Purification; Precipitation; Filtration
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of acquisition methods of haematochrome, comprising: the impurity in removal safflower raw material；Safflower raw material is dried in an oven；Dry safflower raw material is placed in sterilization container and is sterilized；3~10 times of water is added in safflower raw material after sterilization, first time separation of solid and liquid is carried out after stirring 0.5~3h, 3~6 times of water is added in the solid after first time is separated by solid-liquid separation, second of separation of solid and liquid is carried out after stirring 1.5~3.5h, it will be separated by solid-liquid separation obtained liquid for the first time and be separated by solid-liquid separation obtained liquid for the second time and merge, obtain Flos Carthami extract；The ethyl alcohol that concentration is 45%~70% is added in Flos Carthami extract to be eluted, eluent is obtained；Concentration is used to carry out pH value adjustment for 0.6~0.95% glacial acetic acid eluent, so that the pH value of eluent is 2~5；It is concentrated after eluent is adjusted to neutrality, obtains haematochrome.The present invention improves the content of haematochrome in extract.
Description
Technical field
The present invention relates to technical field of plant extraction, in particular to a kind of acquisition methods of haematochrome.
Background technique
Safflower is the important Chinese herbal medicine of China, is the dry flower of compositae plant, includes safflower red pigment and two kinds of haematochrome
Pigment, warm-natured, acrid flavour, there is blood circulation, stasis-dispelling and pain-killing, reduce cholesterol, blood pressure lowering and other effects, it is mainly used for menstruation not
Tune, dysmenorrhea, menostasis, traumatic injury also have certain curative effect to diseases such as coronary heart disease, catarrhal jaundices.Safflower carthamin (Chinese medicine
It is II class, freeze-dried) it is a kind of water-soluble flavone class compound extracted from safflower, pharmacodynamics documents and materials report: safflower has
Coronary resistance is reduced, the effect of coronary flow and myocardial nutrition is increased.In the prior art, safflower red pigment is extracted from safflower
Process flow generally includes extracting and developing, purifying and preparation, but the content for the safflower red pigment for using the method to obtain is lower.
Summary of the invention
In view of this, the present invention provides a kind of acquisition methods of haematochrome, which raises the contents of haematochrome in extract.
The present invention is solved the above problems by following technological means:
A kind of acquisition methods of haematochrome of the invention, comprising the following steps:, removal safflower raw material in impurity；, will go
It is dried except the safflower raw material after impurity is placed in furnace；, the safflower raw material after drying be placed in sterilization container carry out
Sterilization；3~10 times of water is added in safflower raw material after sterilization, progress first time separation of solid and liquid after 0.5~3h of stirring, then
The water of 3~6 times of solids is added in solid after being separated by solid-liquid separation for the first time, carries out second after 1.5~3.5h of stirring and is separated by solid-liquid separation,
It will finally be separated by solid-liquid separation obtained liquid for the first time and be separated by solid-liquid separation obtained liquid for the second time and merge, obtain safflower extraction
Liquid；The ethyl alcohol that concentration is 45%~70% is added in Flos Carthami extract to be eluted, eluent is obtained；The eluent is adopted
PH value adjustment is carried out with the glacial acetic acid that concentration is 0.6~0.95%, so that the pH value of eluent is 2~5；After adjusting pH value
Eluent is concentrated after being adjusted to neutrality, and obtains haematochrome.
Further, the safflower raw material after the impurity by removal, which is placed on to be dried in furnace, specifically includes:
Safflower raw material after removal impurity is placed in the furnace that temperature is 60 DEG C and is dried.
Further, the safflower raw material by after drying is placed in sterilization container sterilize and specifically include:
Safflower raw material after drying is placed in ozone sterilization container and is sterilized.
Further, it is described by the eluent use concentration for 0.6~0.95% glacial acetic acid carry out pH value adjustment it
Before, further includes:
The eluent is stood 3~5 hours at normal temperature.
Further, it is described by the eluent use concentration for 0.6~0.95% glacial acetic acid carry out pH value adjustment include:
It is concentrated after eluent after standing at normal temperature is adjusted to neutrality, obtains haematochrome.
A kind of acquisition methods of haematochrome of the invention have the advantages that
It is red after impurity, drying and sterilization by being successively removed the present invention provides a kind of acquisition methods of haematochrome
After water is added in flower raw material, be separated by solid-liquid separation for the first time and second is separated by solid-liquid separation, the liquid obtained after being finally separated by solid-liquid separation twice
Body merges, and obtains Flos Carthami extract, and certain density ethyl alcohol is then added in Flos Carthami extract and is eluted, is washed
De- liquid, and use glacial acetic acid by the pH value adjustment of eluent for 2~5, then after the eluent after adjusting pH value is adjusted to neutrality
It is concentrated, obtains haematochrome, improve the content of haematochrome in extract.
Detailed description of the invention
The invention will be further described with reference to the accompanying drawings and examples.
Fig. 1 is a kind of flow diagram of the acquisition methods of haematochrome of the invention.
Specific embodiment
The acquisition methods of a kind of haematochrome of the invention, as shown in Figure 1, mainly comprising the steps that
101, the impurity in safflower raw material is removed.
102, the safflower raw material after removal impurity is placed in furnace and is dried.
It is dried specifically, the safflower raw material after removal impurity is placed in the furnace that temperature is 60 DEG C.
103, the safflower raw material after drying is placed in sterilization container and is sterilized.
It is sterilized specifically, the safflower raw material after drying is placed in ozone sterilization container.
104,3~10 times of water is added in safflower raw material after sterilization, carries out first time solid-liquid point after stirring 0.5~3h
From then the water of 3~6 times of solids being added in the solid after first time is separated by solid-liquid separation, stirs that carry out second after 1.5~3.5h solid
Liquid separation, will finally be separated by solid-liquid separation obtained liquid for the first time and is separated by solid-liquid separation obtained liquid for the second time and merges, and obtain
Flos Carthami extract.
105, the ethyl alcohol that concentration is 45%~70% is added in Flos Carthami extract to be eluted, eluent is obtained.
106, concentration is used to carry out pH value adjustment for 0.6~0.95% glacial acetic acid the eluent, so that eluent
PH value be 2~5.
107, it is concentrated after being adjusted to neutrality the eluent after adjusting pH value, obtains haematochrome.
It should be noted that before step 5 and step 6 further include:
The eluent is stood 3~5 hours at normal temperature.
It is described by the eluent use concentration for 0.6~0.95% glacial acetic acid carry out pH value adjustment include:
It is concentrated after eluent after standing at normal temperature is adjusted to neutrality, obtains haematochrome.
It is red after impurity, drying and sterilization by being successively removed the present invention provides a kind of acquisition methods of haematochrome
After water is added in flower raw material, be separated by solid-liquid separation for the first time and second is separated by solid-liquid separation, the liquid obtained after being finally separated by solid-liquid separation twice
Body merges, and obtains Flos Carthami extract, and certain density ethyl alcohol is then added in Flos Carthami extract and is eluted, is washed
De- liquid, and use glacial acetic acid by the pH value adjustment of eluent for 2~5, then after the eluent after adjusting pH value is adjusted to neutrality
It is concentrated, obtains haematochrome, improve the content of haematochrome in extract.
Embodiment one
Step 1: the impurity in removal safflower raw material.
Step 2: the safflower raw material after removal impurity being placed in the furnace that temperature is 60 DEG C and is dried.
Step 3: the safflower raw material after drying being placed in ozone sterilization container and is sterilized.
Step 4: 4 times of water is added in safflower raw material after sterilization, carries out first time separation of solid and liquid after stirring 0.5h, then
The water of 3.5 times of solids is added in the solid after first time is separated by solid-liquid separation, carries out second after stirring 1.5h and is separated by solid-liquid separation, finally
It will be separated by solid-liquid separation obtained liquid for the first time and be separated by solid-liquid separation obtained liquid for the second time and merge, obtain Flos Carthami extract.
Step 5: the ethyl alcohol that concentration is 45%~70% being added in Flos Carthami extract and is eluted, eluent is obtained.
Step 6: using concentration to carry out pH value adjustment for 0.75% glacial acetic acid the eluent, so that the PH of eluent
Value is 2.
Step 7: being concentrated after the eluent after adjusting pH value is adjusted to neutrality, obtain haematochrome.
Obtained haematochrome is detected, testing result is as follows:
The content of haematochrome is 76% in the extract obtained by embodiment one.
Embodiment two
Step 1: the impurity in removal safflower raw material.
Step 2: the safflower raw material after removal impurity being placed in the furnace that temperature is 60 DEG C and is dried.
Step 3: the safflower raw material after drying being placed in ozone sterilization container and is sterilized.
Step 4: 5 times of water is added in safflower raw material after sterilization, carries out first time separation of solid and liquid after stirring 2.5h, then
The water of 3 times of solids is added in the solid after first time is separated by solid-liquid separation, stirs and carries out second after 3h and be separated by solid-liquid separation, finally by the
It is once separated by solid-liquid separation obtained liquid and is separated by solid-liquid separation obtained liquid for the second time and merge, obtain Flos Carthami extract.
Step 5: the ethyl alcohol that concentration is 70% being added in Flos Carthami extract and is eluted, eluent is obtained.
Step 6: using concentration to carry out pH value adjustment for 0.9% glacial acetic acid the eluent, so that the PH of eluent
Value is 5.
Step 7: being concentrated after the eluent after adjusting pH value is adjusted to neutrality, obtain haematochrome.
Obtained haematochrome is detected, testing result is as follows:
The content of haematochrome is 92% in the extract obtained by embodiment two.
Finally, it is stated that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although referring to compared with
Good embodiment describes the invention in detail, those skilled in the art should understand that, it can be to skill of the invention
Art scheme is modified or replaced equivalently, and without departing from the objective and range of technical solution of the present invention, should all be covered at this
In the scope of the claims of invention.
Claims (5)
1. a kind of acquisition methods of haematochrome, it is characterised in that: the following steps are included:
Remove the impurity in safflower raw material；
Safflower raw material after removal impurity is placed in furnace and is dried；
Safflower raw material after drying is placed in sterilization container and is sterilized；
3~10 times of water is added in safflower raw material after sterilization, progress first time separation of solid and liquid after 0.5~3h of stirring, then
The water of 3~6 times of solids is added in solid after being separated by solid-liquid separation for the first time, carries out second after 1.5~3.5h of stirring and is separated by solid-liquid separation,
It will finally be separated by solid-liquid separation obtained liquid for the first time and be separated by solid-liquid separation obtained liquid for the second time and merge, obtain safflower extraction
Liquid；
The ethyl alcohol that concentration is 45%~70% is added in Flos Carthami extract to be eluted, eluent is obtained；
Concentration is used to carry out pH value adjustment for 0.6~0.95% glacial acetic acid the eluent, so that the pH value of eluent is 2
~5；
It is concentrated after eluent after adjusting pH value is adjusted to neutrality, obtains haematochrome.
2. a kind of acquisition methods of haematochrome according to claim 1, it is characterised in that: red after the impurity by removal
Flower raw material, which is placed on to be dried in furnace, to be specifically included:
Safflower raw material after removal impurity is placed in the furnace that temperature is 60 DEG C and is dried.
3. a kind of acquisition methods of haematochrome according to claim 2, it is characterised in that: described that safflower after drying is former
Material is placed in sterilization container sterilize and specifically include:
Safflower raw material after drying is placed in ozone sterilization container and is sterilized.
4. a kind of acquisition methods of haematochrome according to claim 1, it is characterised in that: adopt the eluent described
Before carrying out pH value adjustment with the glacial acetic acid that concentration is 0.6~0.95%, further includes:
The eluent is stood 3~5 hours at normal temperature.
5. a kind of acquisition methods of haematochrome according to claim 4, it is characterised in that: described to use the eluent
The glacial acetic acid that concentration is 0.6~0.95% carries out pH value adjustment
It is concentrated after eluent after standing at normal temperature is adjusted to neutrality, obtains haematochrome.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710839072.9A CN109517408A (en) | 2017-09-16 | 2017-09-16 | A kind of acquisition methods of haematochrome |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710839072.9A CN109517408A (en) | 2017-09-16 | 2017-09-16 | A kind of acquisition methods of haematochrome |
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| Publication Number | Publication Date |
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| CN109517408A true CN109517408A (en) | 2019-03-26 |
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ID=65769557
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| CN201710839072.9A Pending CN109517408A (en) | 2017-09-16 | 2017-09-16 | A kind of acquisition methods of haematochrome |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115044227A (en) * | 2022-06-22 | 2022-09-13 | 温州大学 | Method for preparing traditional plant dye safflower cake by using natural acid agent |
-
2017
- 2017-09-16 CN CN201710839072.9A patent/CN109517408A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115044227A (en) * | 2022-06-22 | 2022-09-13 | 温州大学 | Method for preparing traditional plant dye safflower cake by using natural acid agent |
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Application publication date: 20190326 |