CN102657691A - Extraction process of carthamin yellow - Google Patents
Extraction process of carthamin yellow Download PDFInfo
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- CN102657691A CN102657691A CN2012101794752A CN201210179475A CN102657691A CN 102657691 A CN102657691 A CN 102657691A CN 2012101794752 A CN2012101794752 A CN 2012101794752A CN 201210179475 A CN201210179475 A CN 201210179475A CN 102657691 A CN102657691 A CN 102657691A
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Abstract
The invention discloses an extraction process of medicinal components of a Chinese herbal medicine safflower and particularly relates to an extraction process of carthamin yellow. The extraction process comprises the following steps: extraction of medicinal materials, adsorption by virtue of a macroporous adsorption resin column, primary elution, adsorption by virtue of a polyamide column, secondary elution, concentration and spray-drying. The extraction process of carthamin yellow can be used for effectively solving the disadvantages existing in the prior art of the background technology, the yield of the extracted carthamin yellow is not less than 2%, hydroxyl safflower flavin A contained in the carthamin yellow is not less than 20%, and general flavone contained in the carthamin yellow is not less than 80%.
Description
Technical field
The invention discloses a kind of extraction process of Chinese crude drug Flos Carthami medicinal ingredient, specifically relate to a kind of Carthamus yellow extraction process.
Background technology
Carthamus yellow, function cures mainly blood circulation promoting and blood stasis dispelling, and coronary circulation-promoting pain-relieving is used for the stable type fatigue property angina pectoris of I that stagnation of heart-blood causes, II, III level, and disease sees that chest pain is uncomfortable in chest, and is nervous, breathes hard etc.
At present,, report comparatively extensive about the extraction process of Carthamus yellow, but problem such as ubiquity extraction process length consuming time, energy consumption are high, yield is low.
Summary of the invention
In order to solve the problems of the technologies described above, the object of the present invention is to provide a kind of Carthamus yellow extraction process, can effectively address the above problem, yield and quality are all higher.
The technical scheme that the present invention adopts is following:
The Carthamus yellow extraction process is characterized in that, comprise the absorption of medicinal material extract, macroporous adsorptive resins, for the first time eluting, polyamide column absorption, for the second time eluting, concentrate and spray drying, wherein,
Medicinal material extract is got flos carthami, adds the water of 8～10 times of medical material weight, and 69～71 ℃ are stirred extraction 2 times, and each 1～1.5 hour, filter, merge extracted twice liquid, be cooled to room temperature;
Macroporous adsorptive resins absorption is 1: 5 ratio according to upper prop sample size and amount of resin, adsorbs in the flow velocity adding macroporous adsorptive resins with 2～2.5bV/h;
Eluting uses 50% ethanol as eluent for the first time, and with 9 bed volumes of flow velocity eluting of 3～3.5bV/h, it is 3.5～4 that eluent uses 0.8% glacial acetic acid to transfer to pH;
Polyamide column absorption is 1: 15 ratio according to upper prop sample size and polyamide amount, is added in the polyamide column and adsorbs;
Eluting for the second time, adopting 50% ethanol successively, using 0.8% glacial acetic acid to regulate pH is that 3.5～4 water, purified water and 70% ethanol carry out eluting, 1.5,1.5,3,2 bed volumes of eluting are collected eluent respectively;
Concentrate, eluent concentrates with the PS membrane ultrafilter, and molecular cut off is 300, carries out spray drying after concentrating, and gets orange-yellow Carthamus yellow.
Preferably, eluent concentrates the back, and to adopt molecular cut off be that 10000 PS membrane ultrafilter carries out ultrafiltration, and ultrafiltrate carries out spray drying again.
Further, said polyamide particle diameter is 90 orders.
Further, in the eluting operation for the second time, it is 3.5～4 that 50% ethanol uses 0.8% glacial acetic acid to regulate pH.
Comparatively perfect is that Carthamus yellow hydroxyl safflor yellow A is no less than 20%, contains total flavones and is no less than 80%.
Carthamus yellow extraction process of the present invention effectively solves the drawback that prior art exists in the background technology, the Carthamus yellow of extraction, and yield is no less than 2%, and Carthamus yellow hydroxyl safflor yellow A is no less than 20%, contains total flavones and is no less than 80%.
The specific embodiment
Below in conjunction with embodiment the present invention is further described, but the present invention is not limited only to these embodiment, is not breaking away under the prerequisite of aim of the present invention, any improvement of being done all drops within protection scope of the present invention.
The Carthamus yellow extraction process, step is following:
1, medicinal material extract is got flos carthami, adds the water of 8～10 times of medical material weight, and 69～71 ℃ are stirred extraction 2 times, and each 1～1.5 hour, filter, merge extracted twice liquid, be cooled to room temperature.
2, macroporous adsorptive resins absorption, according to upper prop sample size and amount of resin be 1: 5 (W: ratio V), adsorb in the flow velocity adding macroporous adsorptive resins with 2～2.5bV/h.
3, eluting for the first time uses 50% (volumn concentration) ethanol as eluent, and with 9 bed volumes of flow velocity eluting of 3～3.5bV/h, it is 3.5～4 that eluent transfers to pH with 0.8% (volumn concentration) glacial acetic acid.
4, polyamide column absorption is that (W: ratio V) was added in polyamide (90 order) post and adsorbs in 1: 15 according to upper prop sample size and polyamide amount.
5, eluting for the second time; Adopting 50% (volumn concentration) ethanol successively, using 0.8% (volumn concentration) glacial acetic acid to regulate pH is that 3.5～4 water, purified water and 70% (volumn concentration) ethanol carry out eluting; 1.5,1.5,3,2 bed volumes of difference eluting; Collect eluent, wherein 50% (volumn concentration) ethanol is 3.5～4 with 0.8% (volumn concentration) glacial acetic acid adjusting pH.
6, concentrate, eluent concentrates with the PS membrane ultrafilter, and molecular cut off is 300.
7, eluent concentrates the back to adopt molecular cut off is that 10000 PS membrane ultrafilter carries out ultrafiltration.
8, ultrafiltrate carries out spray drying again, gets orange-yellow Carthamus yellow, and yield is no less than 2%, and Carthamus yellow hydroxyl safflor yellow A is no less than 20%, contains total flavones and is no less than 80%.
Above content only is to give an example and explanation to what process route of the present invention was done; Under the technical staff in present technique field described specific embodiment is made various modifications or replenish or adopt similar mode to substitute; Only otherwise depart from the design of invention or surmount the defined scope of these claims, all should belong to protection scope of the present invention.
Claims (5)
1. the Carthamus yellow extraction process is characterized in that, comprise the absorption of medicinal material extract, macroporous adsorptive resins, for the first time eluting, polyamide column absorption, for the second time eluting, concentrate and spray drying, wherein,
Medicinal material extract is got flos carthami, adds the water of 8～10 times of medical material weight, and 69～71 ℃ are stirred extraction 2 times, and each 1～1.5 hour, filter, merge extracted twice liquid, be cooled to room temperature;
Macroporous adsorptive resins absorption is 1: 5 ratio according to upper prop sample size and amount of resin, adsorbs in the flow velocity adding macroporous adsorptive resins with 2～2.5bV/h;
Eluting uses 50% ethanol as eluent for the first time, and with 9 bed volumes of flow velocity eluting of 3～3.5bV/h, it is 3.5～4 that eluent uses 0.8% glacial acetic acid to transfer to pH;
Polyamide column absorption is 1: 15 ratio according to upper prop sample size and polyamide amount, is added in the polyamide column and adsorbs;
Eluting for the second time, adopting 50% ethanol successively, using 0.8% glacial acetic acid to regulate pH is that 3.5～4 water, purified water and 70% ethanol carry out eluting, 1.5,1.5,3,2 bed volumes of eluting are collected eluent respectively;
Concentrate, eluent concentrates with the PS membrane ultrafilter, and molecular cut off is 300, carries out spray drying after concentrating, and gets orange-yellow Carthamus yellow.
2. Carthamus yellow extraction process according to claim 1 is characterized in that, eluent concentrates the back, and to adopt molecular cut off be that 10000 PS membrane ultrafilter carries out ultrafiltration, and ultrafiltrate carries out spray drying again.
3. Carthamus yellow extraction process according to claim 1 is characterized in that, said polyamide particle diameter is 90 orders.
4. Carthamus yellow extraction process according to claim 1 is characterized in that, in the eluting operation for the second time, it is 3.5～4 that 50% ethanol uses 0.8% glacial acetic acid to regulate pH.
5. according to each described Carthamus yellow extraction process of claim 1～4, it is characterized in that Carthamus yellow hydroxyl safflor yellow A is no less than 20%, contains total flavones and is no less than 80%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2012101794752A CN102657691A (en) | 2012-06-02 | 2012-06-02 | Extraction process of carthamin yellow |
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| CN2012101794752A CN102657691A (en) | 2012-06-02 | 2012-06-02 | Extraction process of carthamin yellow |
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| CN2012101794752A Pending CN102657691A (en) | 2012-06-02 | 2012-06-02 | Extraction process of carthamin yellow |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106189352A (en) * | 2016-07-19 | 2016-12-07 | 广州中大南沙科技创新产业园有限公司 | A kind of extracting method of Carthamus yellow |
| CN108226073A (en) * | 2016-12-15 | 2018-06-29 | 江苏维赛科技生物发展有限公司 | A kind of method that the content of general flavone in the safflower of Xinjiang is measured using spectrophotometry |
| CN111362896A (en) * | 2018-12-25 | 2020-07-03 | 浙江永宁药业股份有限公司 | Safflower yellow and its application in preparing medicine for treating cardiovascular and cerebrovascular diseases |
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| CN1272500A (en) * | 1998-11-24 | 2000-11-08 | 北京市心肺血管疾病研究所 | Safflower total uranidin, its preparation and application |
| CN1368503A (en) * | 2000-10-30 | 2002-09-11 | 山东绿叶制药股份有限公司 | Safflower lyochrome rich in carthamin A, its preparing process, its preparation and its medical usage |
| CN1785988A (en) * | 2004-12-06 | 2006-06-14 | 山东绿叶天然药物研究开发有限公司 | Saflor yellow pigment rich in saflor yellow pigment B, its preparation method and use |
| JP2008163092A (en) * | 2006-12-27 | 2008-07-17 | Toyo Ink Mfg Co Ltd | Safflower-colored fine particle and preparation |
| CN101322693A (en) * | 2008-07-31 | 2008-12-17 | 浙江永宁药业股份有限公司 | Carthamus tinctorius yellow colour injection and preparation technique thereof |
| CN102382151A (en) * | 2011-09-08 | 2012-03-21 | 成都中医药大学 | Separation and purification method for safflower yellow |
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2012
- 2012-06-02 CN CN2012101794752A patent/CN102657691A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1272500A (en) * | 1998-11-24 | 2000-11-08 | 北京市心肺血管疾病研究所 | Safflower total uranidin, its preparation and application |
| CN1368503A (en) * | 2000-10-30 | 2002-09-11 | 山东绿叶制药股份有限公司 | Safflower lyochrome rich in carthamin A, its preparing process, its preparation and its medical usage |
| CN1785988A (en) * | 2004-12-06 | 2006-06-14 | 山东绿叶天然药物研究开发有限公司 | Saflor yellow pigment rich in saflor yellow pigment B, its preparation method and use |
| JP2008163092A (en) * | 2006-12-27 | 2008-07-17 | Toyo Ink Mfg Co Ltd | Safflower-colored fine particle and preparation |
| CN101322693A (en) * | 2008-07-31 | 2008-12-17 | 浙江永宁药业股份有限公司 | Carthamus tinctorius yellow colour injection and preparation technique thereof |
| CN102382151A (en) * | 2011-09-08 | 2012-03-21 | 成都中医药大学 | Separation and purification method for safflower yellow |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106189352A (en) * | 2016-07-19 | 2016-12-07 | 广州中大南沙科技创新产业园有限公司 | A kind of extracting method of Carthamus yellow |
| CN106189352B (en) * | 2016-07-19 | 2018-06-26 | 广州中大南沙科技创新产业园有限公司 | A kind of extracting method of carthamin yellow |
| CN108226073A (en) * | 2016-12-15 | 2018-06-29 | 江苏维赛科技生物发展有限公司 | A kind of method that the content of general flavone in the safflower of Xinjiang is measured using spectrophotometry |
| CN111362896A (en) * | 2018-12-25 | 2020-07-03 | 浙江永宁药业股份有限公司 | Safflower yellow and its application in preparing medicine for treating cardiovascular and cerebrovascular diseases |
| CN111362896B (en) * | 2018-12-25 | 2022-10-04 | 浙江永宁药业股份有限公司 | Safflower yellow and its application in preparing medicine for treating cardiovascular and cerebrovascular diseases |
| CN115677638A (en) * | 2018-12-25 | 2023-02-03 | 浙江永宁药业股份有限公司 | Safflower yellow and application thereof in preparing medicine for treating cardiovascular and cerebrovascular diseases |
| CN115677638B (en) * | 2018-12-25 | 2023-11-14 | 浙江永宁药业股份有限公司 | Carthamin yellow and application thereof in preparing medicine for treating cardiovascular and cerebrovascular diseases |
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Application publication date: 20120912 |