CN106189352A - A kind of extracting method of Carthamus yellow - Google Patents
A kind of extracting method of Carthamus yellow Download PDFInfo
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- CN106189352A CN106189352A CN201610570659.XA CN201610570659A CN106189352A CN 106189352 A CN106189352 A CN 106189352A CN 201610570659 A CN201610570659 A CN 201610570659A CN 106189352 A CN106189352 A CN 106189352A
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- flos carthami
- eluent
- carthamus yellow
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- dried
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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Abstract
The invention discloses the extracting method of a kind of Carthamus yellow, including: step 1: remove the impurity in Flos Carthami raw material；Step 2: be dried；Step 3: sterilization；Step 4: add the water of 5～16 times of Flos Carthami raw materials quality in Flos Carthami raw material after sterilization, carries out adding in first time solid-liquid separation, then the solid after first time solid-liquid separation the water of 2～5 times of solids, carries out second time solid-liquid separation, obtains Flos Carthami extract；Step 5: add ethanol in Flos Carthami extract and carry out eluting for the first time, obtain the first eluent；Step 6: use glacial acetic acid to carry out pH value regulation the first eluent；Step 7: add polyamide in the first eluent and adsorb；Step 8: add ethanol in the first eluent after absorption and carry out second time eluting, obtain the second eluent；Step 9: the second eluent regulation is concentrated to neutrality, obtains Carthamus yellow.The present invention improves the content of Carthamus yellow in extract.
Description
Technical field
The present invention relates to pharmaceutical technology field, particularly to the extracting method of a kind of Carthamus yellow.
Background technology
Flos Carthami is the Chinese herbal medicine that China is important, for the dry flower of feverfew, includes safflower red pigment Flos Carthami flavochrome
Two kinds of pigments, its warm in nature, acrid in the mouth, there is the effects such as promoting blood circulation to restore menstrual flow, stasis-dispelling and pain-killing, reduction cholesterol, blood pressure lowering, be mainly used in the moon
Through uncomfortable, dysmenorrhea, amenorrhea, traumatic injury, the disease such as coronary heart disease, infectious hepatitis also there is certain curative effect.Carthamus yellow
It is the natural yellow pigment extracted from the petal of Flos Carthami, for Chalcone Compounds.Research finds that Carthamus yellow is not only
A kind of of great value natural food colour, has beautiful in colour, reduction high temperature resistant, high pressure resistant, low temperature resistant, fast light, acidproof, resistance to
With the advantage such as antimicrobial, and also coronary artery dilator, antioxidation, protection cardiac muscle, blood pressure lowering, immunosuppressant and brain protection
Etc. multiple pharmacological function.In prior art, extract from Flos Carthami the technological process of Carthamus yellow generally include extracting and developing,
Purification and preparation, but the content of Carthamus yellow using the method to obtain is relatively low.
Summary of the invention
In view of this, the present invention provides the extracting method of a kind of Carthamus yellow, which raises Flos Carthami yellow in extract
The content of element.
The present invention solves the problems referred to above by techniques below means:
The extracting method of a kind of Carthamus yellow of the present invention, comprises the following steps: step 1: remove in Flos Carthami raw material
Impurity；Step 2: the Flos Carthami raw material after going the removal of impurity is placed in stove and is dried；Step 3: by dried Flos Carthami raw material
It is placed in sterilization container and sterilizes；Step 4: add 5～16 times of Flos Carthami raw materials quality in Flos Carthami raw material after sterilization
Water, carries out adding in first time solid-liquid separation, then the solid after first time solid-liquid separation 2～5 times of solids after stirring 1～2h
Water, carries out second time solid-liquid separation, the liquid finally first time solid-liquid separation obtained and second time solid-liquid and divides after stirring 2～3h
Merge from the liquid obtained, obtain Flos Carthami extract；Step 5: adding concentration in Flos Carthami extract is 60%～80%
Ethanol carry out for the first time eluting, obtain the first eluent；Step 6: by described first eluent use concentration be 0.7～
The glacial acetic acid of 0.9% carries out pH value regulation so that the pH value of the first eluent is 2～5；Step 7: first that pH value is 2～5
Eluent adds the polyamide of preset ratio, adsorbs in polyamide column；Step 8: the first eluent after absorption
Middle addition concentration be 60%～80% ethanol carry out second time eluting, obtain the second eluent；Step 9: by the second eluent
Regulation concentrates to neutrality, obtains Carthamus yellow.
Further, in described step 2 will go the removal of impurity after Flos Carthami raw material be placed in stove to be dried and specifically include:
Flos Carthami raw material after going the removal of impurity is placed in the stove that temperature is 45 DEG C and is dried.
Further, being placed in sterilization container by dried Flos Carthami raw material in described step 3 carries out the concrete bag that sterilizes
Include: dried Flos Carthami raw material is placed in ultraviolet sterilization container and sterilizes.
Further, described, second eluent regulation is concentrated to neutrality, after obtaining Carthamus yellow, also wrap
Include: step 10: the Carthamus yellow obtained is dried, and dried Carthamus yellow is placed in storage tank deposits
Storage.
The extracting method of a kind of Carthamus yellow of the present invention has the advantages that
The invention provides the extracting method of a kind of Carthamus yellow, impurity will be removed successively, be dried and after sterilization
Flos Carthami raw material add after water, carry out solid-liquid separation and second time solid-liquid separation for the first time, obtain after last twice solid-liquid separation
Liquid merge, obtain Flos Carthami extract, in Flos Carthami extract, then add certain density ethanol carry out for the first time
Eluting, obtains the first eluent, and uses certain density glacial acetic acid that the pH value of the first eluent is adjusted to 2～5, then poly-
Amide post adsorbs, the first eluent after absorption finally adds certain density ethanol and carries out second time eluting,
Obtain the second eluent, then the second eluent regulation is concentrated to neutrality, finally give Carthamus yellow, in employing
The extracting method stating Carthamus yellow can get the Carthamus yellow that content is higher, i.e. improves Carthamus yellow in extract
Content.
Detailed description of the invention
The extracting method of a kind of Carthamus yellow of the present invention, mainly comprises the steps that
Step 1: remove the impurity in Flos Carthami raw material.
Step 2: the Flos Carthami raw material after going the removal of impurity is placed in stove and is dried.
Concrete, the Flos Carthami raw material after going the removal of impurity is placed in the stove that temperature is 45 DEG C and is dried.
Step 3: dried Flos Carthami raw material is placed in sterilization container and sterilizes.
Concrete, dried Flos Carthami raw material is placed in ultraviolet sterilization container and sterilizes.
Step 4: add the water of 5～16 times of Flos Carthami raw materials quality in Flos Carthami raw material after sterilization, carry out after stirring 1～2h
Solid-liquid separation for the first time, then the solid after first time solid-liquid separation add the water of 2～5 times of solids, carries out after stirring 2～3h
The liquid that solid-liquid separation for the second time, the liquid finally first time solid-liquid separation obtained and second time solid-liquid separation obtain closes
And, obtain Flos Carthami extract.
Step 5: add the ethanol that concentration is 60%～80% in Flos Carthami extract and carry out eluting for the first time, obtain first
Eluent.
Step 6: described first eluent is used concentration be 0.7～0.9% glacial acetic acid carry out pH value regulation so that the
The pH value of one eluent is 2～5.
Step 7: add the polyamide of preset ratio in the first eluent that pH value is 2～5, carry out in polyamide column
Absorption.
Step 8: add the ethanol that concentration is 60%～80% in the first eluent after absorption and carry out second time eluting,
Obtain the second eluent.
Step 9: the second eluent regulation is concentrated to neutrality, obtains Carthamus yellow.
Step 10: the Carthamus yellow obtained is dried, and dried Carthamus yellow is placed in storage tank
Store.
The invention provides the extracting method of a kind of Carthamus yellow, impurity will be removed successively, be dried and after sterilization
Flos Carthami raw material add after water, carry out solid-liquid separation and second time solid-liquid separation for the first time, obtain after last twice solid-liquid separation
Liquid merge, obtain Flos Carthami extract, in Flos Carthami extract, then add certain density ethanol carry out for the first time
Eluting, obtains the first eluent, and uses certain density glacial acetic acid that the pH value of the first eluent is adjusted to 2～5, then poly-
Amide post adsorbs, the first eluent after absorption finally adds certain density ethanol and carries out second time eluting,
Obtain the second eluent, then the second eluent regulation is concentrated to neutrality, finally give Carthamus yellow, in employing
The extracting method stating Carthamus yellow can get the Carthamus yellow that content is higher, i.e. improves Carthamus yellow in extract
Content.
Embodiment one
Step 1: remove the impurity in Flos Carthami raw material.
Step 2: the Flos Carthami raw material after going the removal of impurity is placed in the stove that temperature is 45 DEG C and is dried.
Step 3: dried Flos Carthami raw material is placed in ultraviolet sterilization container and sterilizes.
Step 4: add the water of 5 times of Flos Carthami raw materials quality in Flos Carthami raw material after sterilization, carry out for the first time after stirring 1h
Solid-liquid separation, then the solid after first time solid-liquid separation add the water of 2 times of solids, carries out second time solid-liquid after stirring 2h and divide
From, the liquid that the liquid finally first time solid-liquid separation obtained and second time solid-liquid separation obtain merges, and obtains Flos Carthami
Extracting solution.
Step 5: add the ethanol that concentration is 60% in Flos Carthami extract and carry out eluting for the first time, obtain the first eluting
Liquid.
Step 6: the glacial acetic acid that described first eluent uses concentration to be 0.7% is carried out pH value regulation so that first washes
The pH value of de-liquid is 2.
Step 7: add the polyamide of preset ratio in the first eluent that pH value is 2, inhale in polyamide column
Attached.
Step 8: add the ethanol that concentration is 60% in the first eluent after absorption and carry out second time eluting, obtain the
Two eluents.
Step 9: the second eluent regulation is concentrated to neutrality, obtains Carthamus yellow.
Step 10: the Carthamus yellow obtained is dried, and dried Carthamus yellow is placed in storage tank
Store.
Detecting the Carthamus yellow obtained, testing result is as follows:
It is 87% by the content of Carthamus yellow in the extract that embodiment one obtains.
Embodiment two
Step 1: remove the impurity in Flos Carthami raw material.
Step 2: the Flos Carthami raw material after going the removal of impurity is placed in the stove that temperature is 45 DEG C and is dried.
Step 3: dried Flos Carthami raw material is placed in ultraviolet sterilization container and sterilizes.
Step 4: add the water of 10 times of Flos Carthami raw materials quality in Flos Carthami raw material after sterilization, carry out first after stirring 1.5h
Secondary solid-liquid separation, then the solid after first time solid-liquid separation add the water of 3 times of solids, carries out second time after stirring 2.5h solid
Liquid separates, and the liquid that the liquid finally first time solid-liquid separation obtained and second time solid-liquid separation obtain merges, and obtains
Flos Carthami extract.
Step 5: add the ethanol that concentration is 70% in Flos Carthami extract and carry out eluting for the first time, obtain the first eluting
Liquid；
Step 6: the glacial acetic acid that described first eluent uses concentration to be 0.8% is carried out pH value regulation so that first washes
The pH value of de-liquid is 4.
Step 7: add the polyamide of preset ratio in the first eluent that pH value is 4, inhale in polyamide column
Attached.
Step 8: add the ethanol that concentration is 70% in the first eluent after absorption and carry out second time eluting, obtain the
Two eluents.
Step 9: the second eluent regulation is concentrated to neutrality, obtains Carthamus yellow.
Step 10: the Carthamus yellow obtained is dried, and dried Carthamus yellow is placed in storage tank
Store.
Detecting the Carthamus yellow obtained, testing result is as follows:
It is 94% by the content of Carthamus yellow in the extract that embodiment two obtains.
Finally illustrating, above example is only in order to illustrate technical scheme and unrestricted, although with reference to relatively
The present invention has been described in detail by good embodiment, it will be understood by those within the art that, can be to the skill of the present invention
Art scheme is modified or equivalent, and without deviating from objective and the scope of technical solution of the present invention, it all should be contained at this
In the middle of the right of invention.
Claims (4)
1. the extracting method of a Carthamus yellow, it is characterised in that: comprise the following steps:
Step 1: remove the impurity in Flos Carthami raw material；
Step 2: the Flos Carthami raw material after going the removal of impurity is placed in stove and is dried；
Step 3: dried Flos Carthami raw material is placed in sterilization container and sterilizes；
Step 4: add the water of 5～16 times of Flos Carthami raw materials quality in Flos Carthami raw material after sterilization, carry out first after stirring 1～2h
Secondary solid-liquid separation, then the solid after first time solid-liquid separation add the water of 2～5 times of solids, after stirring 2～3h, carries out second
The liquid that secondary solid-liquid separation, the liquid finally first time solid-liquid separation obtained and second time solid-liquid separation obtain merges,
Obtain Flos Carthami extract；
Step 5: add the ethanol that concentration is 60%～80% in Flos Carthami extract and carry out eluting for the first time, obtain the first eluting
Liquid；
Step 6: by described first eluent use concentration be 0.7～0.9% glacial acetic acid carry out pH value regulation so that first washes
The pH value of de-liquid is 2～5；
Step 7: add the polyamide of preset ratio in the first eluent that pH value is 2～5, inhale in polyamide column
Attached；
Step 8: add the ethanol that concentration is 60%～80% in the first eluent after absorption and carry out second time eluting, obtain
Second eluent；
Step 9: the second eluent regulation is concentrated to neutrality, obtains Carthamus yellow.
The extracting method of a kind of Carthamus yellow the most according to claim 1, it is characterised in that: the general in described step 2
The Flos Carthami raw material after the removal of impurity is gone to be placed in stove to be dried and specifically include:
Flos Carthami raw material after going the removal of impurity is placed in the stove that temperature is 45 DEG C and is dried.
The extracting method of a kind of Carthamus yellow the most according to claim 2, it is characterised in that: the general in described step 3
Dried Flos Carthami raw material is placed in sterilization container and carries out sterilization and specifically include:
Dried Flos Carthami raw material is placed in ultraviolet sterilization container and sterilizes.
The extracting method of a kind of Carthamus yellow the most according to claim 1, it is characterised in that: described by the second eluting
Liquid regulation concentrates to neutrality, after obtaining Carthamus yellow, also includes:
Step 10: the Carthamus yellow obtained is dried, and dried Carthamus yellow is placed in storage tank carries out
Storage.
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| CN201610570659.XA CN106189352B (en) | 2016-07-19 | 2016-07-19 | A kind of extracting method of carthamin yellow |
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| CN201610570659.XA CN106189352B (en) | 2016-07-19 | 2016-07-19 | A kind of extracting method of carthamin yellow |
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|---|---|---|---|---|
| CN1264708A (en) * | 1998-11-24 | 2000-08-30 | 北京市心肺血管疾病研究所 | Total safflower yellow and its preparing process and application |
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| CN1368503A (en) * | 2000-10-30 | 2002-09-11 | 山东绿叶制药股份有限公司 | Safflower lyochrome rich in carthamin A, its preparing process, its preparation and its medical usage |
| CN1422616A (en) * | 2001-12-06 | 2003-06-11 | 长春三真实业有限公司 | Use of hydroxy saflor yellow pigment A in preparing medicine for treating and preventing cardiovascular and cerebrovascular disease |
| CN1429830A (en) * | 2001-12-29 | 2003-07-16 | 山东绿叶制药股份有限公司 | Water-soluble safflower extract and its oral preparation for treating and preventing angiocardiopathy andcerebrova scular disease |
| CN1475272A (en) * | 2002-08-17 | 2004-02-18 | 李盛学 | Sydroxy carthamin A and its preparation method and use |
| CN1785988A (en) * | 2004-12-06 | 2006-06-14 | 山东绿叶天然药物研究开发有限公司 | Saflor yellow pigment rich in saflor yellow pigment B, its preparation method and use |
| CN102657691A (en) * | 2012-06-02 | 2012-09-12 | 悦康药业集团安徽天然制药有限公司 | Extraction process of carthamin yellow |
| CN103479702A (en) * | 2012-06-14 | 2014-01-01 | 北京星昊医药股份有限公司 | Extraction method and application of safflower yellow pigment |
| CN103665930A (en) * | 2013-12-31 | 2014-03-26 | 黔南民族师范学院 | Method of extracting natural edible pigment |
-
2016
- 2016-07-19 CN CN201610570659.XA patent/CN106189352B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1264708A (en) * | 1998-11-24 | 2000-08-30 | 北京市心肺血管疾病研究所 | Total safflower yellow and its preparing process and application |
| CN1272500A (en) * | 1998-11-24 | 2000-11-08 | 北京市心肺血管疾病研究所 | Safflower total uranidin, its preparation and application |
| CN1368503A (en) * | 2000-10-30 | 2002-09-11 | 山东绿叶制药股份有限公司 | Safflower lyochrome rich in carthamin A, its preparing process, its preparation and its medical usage |
| CN1422616A (en) * | 2001-12-06 | 2003-06-11 | 长春三真实业有限公司 | Use of hydroxy saflor yellow pigment A in preparing medicine for treating and preventing cardiovascular and cerebrovascular disease |
| CN1429830A (en) * | 2001-12-29 | 2003-07-16 | 山东绿叶制药股份有限公司 | Water-soluble safflower extract and its oral preparation for treating and preventing angiocardiopathy andcerebrova scular disease |
| CN1475272A (en) * | 2002-08-17 | 2004-02-18 | 李盛学 | Sydroxy carthamin A and its preparation method and use |
| CN1785988A (en) * | 2004-12-06 | 2006-06-14 | 山东绿叶天然药物研究开发有限公司 | Saflor yellow pigment rich in saflor yellow pigment B, its preparation method and use |
| CN102657691A (en) * | 2012-06-02 | 2012-09-12 | 悦康药业集团安徽天然制药有限公司 | Extraction process of carthamin yellow |
| CN103479702A (en) * | 2012-06-14 | 2014-01-01 | 北京星昊医药股份有限公司 | Extraction method and application of safflower yellow pigment |
| CN103665930A (en) * | 2013-12-31 | 2014-03-26 | 黔南民族师范学院 | Method of extracting natural edible pigment |
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| CN106189352B (en) | 2018-06-26 |
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