CN106189349A - A kind of method extracting dyestuff based on Radix seu Folium Alchorneae trewioidis - Google Patents
A kind of method extracting dyestuff based on Radix seu Folium Alchorneae trewioidis Download PDFInfo
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- CN106189349A CN106189349A CN201610559048.5A CN201610559048A CN106189349A CN 106189349 A CN106189349 A CN 106189349A CN 201610559048 A CN201610559048 A CN 201610559048A CN 106189349 A CN106189349 A CN 106189349A
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- Prior art keywords
- radix seu
- seu folium
- alchorneae trewioidis
- folium alchorneae
- dyestuff
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of method extracting dyestuff based on Radix seu Folium Alchorneae trewioidis, with Radix seu Folium Alchorneae trewioidis as raw material, use water, alcohol additional high pressure pulsed electric field methods extracts the dyestuff in Radix seu Folium Alchorneae trewioidis, first the method is to be dried by Radix seu Folium Alchorneae trewioidis, pulverize, obtain Radix seu Folium Alchorneae trewioidis powder, slightly carry through high-pressure pulse electric, cellulase essence is extracted, purification by macroporous resin, lyophilization step extracts the dyestuff in Radix seu Folium Alchorneae trewioidis, this invention improves the efficiency extracting procyanidin from Radix seu Folium Alchorneae trewioidis, the dyestuff utilizing the method to be extracted can be widely applied to skin care item industry, the field such as medicines and health protection and functional food, there is important economic worth.
Description
Technical field
The invention belongs to plant extract technical field of dye, be specifically related to a kind of method extracting dyestuff based on Radix seu Folium Alchorneae trewioidis.
Background technology
Radix seu Folium Alchorneae trewioidis, has another name called Begonia fimbristipula Hance, Radix Angelicae Sinensis dish, GynurabicolorDC, Herba Gynurae bicoloris, Radix seu Folium Alchorneae trewioidis, gynura bicolor.Feverfew.Like raw
Long in dark moist place.The bottom surface of leaf is green, and the back side is aubergine, is referred to as " Radix seu Folium Alchorneae trewioidis ".It contributes to blood fat reducing,
Aid digestion, change intestinal gas.It is the wild herbs can tasted throughout the year.Radix seu Folium Alchorneae trewioidis, whole plant for medical use.Acrid in the mouth, sweet, cool in nature.There is clearing away heat and cooling blood,
The effect of removing toxic substances and promoting subsidence of swelling.Curing mainly hemoptysis, metrorrhagia, traumatic hemorrhage, dysmenorrhea, dysentery, sore swollen toxin, traumatic injury, ulcer is not received
Hold back.In Sichuan " blood skin dish ", there is nourishing blood function.Plant blade face is green, purplish, back side aubergine, belongs to perennial root evergreen
Herbaceous plant, it is to integrate Lay, medicinal and half cultigen viewed and admired, and mouthfeel is tender fine and smooth, has special fragrance, taste
Road is good to eat.Containing abundant pigment in Radix seu Folium Alchorneae trewioidis, can extract as natural dye, but at present dyestuff be extracted for Radix seu Folium Alchorneae trewioidis
Also there is no a kind of concrete method；And the method for plant extract natural dye mainly has following several at present: tradition is organic molten
Agent extraction, extraction with aqueous solution method, supercritical fluid extraction, microwave-assisted extraction etc., conventional organic solvents extraction and
Extraction with aqueous solution method extraction ratio is relatively low, and supercritical fluid extraction cost is high, and microwave-assisted extraction noise is big.
Summary of the invention
In place of it is an object of the invention to overcome above-mentioned the deficiencies in the prior art, it is provided that a kind of based on Radix seu Folium Alchorneae trewioidis extraction dyestuff
Method.The dyestuff utilizing the method to be extracted can be widely applied to the fields such as skin care item industry, medicines and health protection and functional food.
The present invention is a kind of method utilizing solvent additional high pressure pulsed electric field methods to extract the dyestuff in Radix seu Folium Alchorneae trewioidis, passes through
High-pressure pulse electric extracts the extraction efficiency significantly improving natural dye, improves pigment content and purity in dyestuff.
The technical scheme that the present invention provides is:
A kind of method extracting dyestuff based on Radix seu Folium Alchorneae trewioidis, described method comprises the following steps:
1) by Radix seu Folium Alchorneae trewioidis clean, be dried, pulverize after, add the 4-8 times of distilled water weighed, be positioned in high-pressure pulse electric and carry
Take, be filtrated to get the first crude extract and filtering residue；
2) filtering residue adding the 3-5 times of ethanol solution weighed, be positioned in high-pressure pulse electric extraction, it is molten that ethanol is removed in decompression
Liquid, is filtrated to get the second crude extract；
3) the first crude extract and the second crude extract being mixed addition 100-200U/mL cellulase, temperature is 30-40 DEG C,
Stand 0.5-1 hour, obtain extracting solution；
4) being separated through macroporous adsorbent resin by extracting solution, and successively use distilled water, ethanol solution carries out eluting；
5) ethanol solution being removed in eluent decompression, then be placed in 40-50 DEG C of vacuum and concentrate, lyophilization is contaminated
Material, cryodesiccated temperature is generally subzero 30 to subzero 50 DEG C.
Preferably, step 1) in Radix seu Folium Alchorneae trewioidis through pulverizing after cross 100 mesh sieves, it is impossible to the pulverizing again sieved.
Preferably, step 1), 2) described in high-pressure pulse electric electric field intensity be 30-40kV/cm, umber of pulse is 8-
10, step 1) 0.5-1 hour extraction time in high-pressure pulse electric, step 2) extraction time in high-pressure pulse electric
For 1-1.5 hour.
Preferably, step 2) in the ethanol water that ethanol solution is mass fraction 80-90%.
Preferably, step 4) in the weight ratio of distilled water and extracting solution be 1: 10, the weight of ethanol solution and extracting solution
Ratio 1: 5.
Preferably, step 4) described in ethanol solution be mass fraction 50-60% ethanol water.
Preferably, step 4) described in macroporous adsorbent resin be the one in AB-8, S-B, NKA, X-5, D296R.
The present invention at least includes following beneficial effect:
The present invention uses water, ethanol additional high pressure impulse electric field to extract and separates, high-pressure pulse electric during extracting
Process and do not result in the rising of material temperature, can effectively protect the physiologically active of dyestuff, it is ensured that the dyestuff of extraction steady
Qualitative, the short easy operation of present invention time, effectively lower the making composition of dyestuff, and use the security dye of water, ethanol extraction
Can be edible.
Part is embodied by the further advantage of the present invention, target and feature by description below, and part also will be by this
Invention research and practice and be understood by the person skilled in the art.
Detailed description of the invention
In conjunction with the following example, the present invention is described in further detail, to make those skilled in the art with reference to description
Word can be implemented according to this.
Embodiment 1
A kind of method based on Radix seu Folium Alchorneae trewioidis extraction dyestuff comprises the following steps:
1) by Radix seu Folium Alchorneae trewioidis clean, be dried, pulverize after, add 4 times of heavy distilled water, be positioned in high-pressure pulse electric and carry
Take, be filtrated to get the first crude extract and filtering residue；
2) filtering residue being added 3 times of heavy ethanol solution, be positioned in high-pressure pulse electric extraction, it is molten that ethanol is removed in decompression
Liquid, is filtrated to get the second crude extract；
3) the first crude extract and the second crude extract being mixed addition 100U/mL cellulase, temperature is 30 DEG C, stands 0.5
Hour, obtain extracting solution；
4) being separated through macroporous adsorbent resin by extracting solution, and successively use distilled water, ethanol solution carries out eluting；
5) ethanol solution being removed in eluent decompression, then be placed in 40 DEG C of vacuum and concentrate, lyophilization obtains dyestuff.
Embodiment 2
A kind of method based on Radix seu Folium Alchorneae trewioidis extraction dyestuff comprises the following steps:
1) by Radix seu Folium Alchorneae trewioidis clean, be dried, pulverize after Radix seu Folium Alchorneae trewioidis through pulverizing after cross 100 mesh sieves, it is impossible to sieve again
Pulverizing, Radix seu Folium Alchorneae trewioidis powder after sieving adds 8 times of heavy distilled water, be positioned over electric field intensity be 30kV/cm, umber of pulse be 8
High-pressure pulse electric extracts 0.5 hour, is filtrated to get the first crude extract and filtering residue；
2) filtering residue adds mass fraction 80% ethanol water of 5 times of weights, and being positioned over electric field intensity is 30kV/cm, arteries and veins
Strokes per minute be 8 high-pressure pulse electric in extract 1 hour, decompression remove ethanol solution, be filtrated to get the second crude extract；
3) the first crude extract and the second crude extract being mixed addition 200U/mL cellulase, temperature is 40 DEG C, stands 1 little
Time, lyophilization obtains extracting solution；
4) being separated through macroporous adsorbent resin by extracting solution, macroporous adsorbent resin is in AB-8, S-B, NKA, X-5, D296R
One, and successively use distilled water, mass fraction 50% ethanol water solution carries out eluting, the weight of distilled water and extracting solution
Amount ratio is 1: 10, ethanol solution and the weight ratio 1: 5 of extracting solution；
5) ethanol solution being removed in eluent decompression, then be placed in 50 DEG C of vacuum and concentrate, lyophilization obtains dyestuff.
Embodiment 3
A kind of method based on Radix seu Folium Alchorneae trewioidis extraction dyestuff comprises the following steps:
1) by Radix seu Folium Alchorneae trewioidis clean, be dried, pulverize after Radix seu Folium Alchorneae trewioidis through pulverizing after cross 100 mesh sieves, it is impossible to sieve again
Pulverizing, Radix seu Folium Alchorneae trewioidis powder after sieving adds 6 times of heavy distilled water, be positioned over electric field intensity be 40kV/cm, umber of pulse be 10
High-pressure pulse electric extracts 1 hour, is filtrated to get the first crude extract and filtering residue；
2) filtering residue adds mass fraction 90% ethanol water of 4 times of weights, and being positioned over electric field intensity is 40kV/cm, arteries and veins
Strokes per minute be 10 high-pressure pulse electric in extract 1.5 hours, decompression remove ethanol solution, be filtrated to get the second crude extract；
3) the first crude extract and the second crude extract being mixed addition 150U/mL cellulase, temperature is 35 DEG C, stands 0.5
Hour, lyophilization obtains extracting solution；
4) being separated through macroporous adsorbent resin by extracting solution, macroporous adsorbent resin is in AB-8, S-B, NKA, X-5, D296R
One, and successively use distilled water, mass fraction 60% ethanol water solution carries out eluting, the weight of distilled water and extracting solution
Amount ratio is 1: 10, ethanol solution and the weight ratio 1: 5 of extracting solution；
5) ethanol solution being removed in eluent decompression, then be placed in 45 DEG C of vacuum and concentrate, lyophilization obtains dyestuff.
Although embodiment of the present invention are disclosed as above, but it is not restricted in description and embodiment listed
Using, it can be applied to various applicable the field of the invention completely, for those skilled in the art, and can be easily
Realizing other amendment, therefore under the general concept limited without departing substantially from claim and equivalency range, the present invention does not limit
In specific details with shown here as the embodiment with description.
Claims (7)
1. the method extracting dyestuff based on Radix seu Folium Alchorneae trewioidis, it is characterised in that described method comprises the following steps:
1) by Radix seu Folium Alchorneae trewioidis clean, be dried, pulverize after, add the 4-8 times of distilled water weighed, be positioned in high-pressure pulse electric extraction,
It is filtrated to get the first crude extract and filtering residue；
2) filtering residue adding the 3-5 times of ethanol solution weighed, be positioned in high-pressure pulse electric extraction, ethanol solution is removed in decompression,
It is filtrated to get the second crude extract；
3) the first crude extract and the second crude extract being mixed addition 100-200U/mL cellulase, temperature is 30-40 DEG C, stands
0.5-1 hour, obtain extracting solution；
4) being separated through macroporous adsorbent resin by extracting solution, successively use distilled water, ethanol solution carries out eluting；
5) ethanol solution being removed in eluent decompression, then be placed in 40-50 DEG C of vacuum and concentrate, lyophilization obtains dyestuff.
The most according to claim 1 based on Radix seu Folium Alchorneae trewioidis extract dyestuff method, it is characterized in that, step 1) in Radix seu Folium Alchorneae trewioidis pass through
100 mesh sieves are crossed, it is impossible to the pulverizing again sieved after pulverizing.
The most according to claim 1 based on Radix seu Folium Alchorneae trewioidis extract dyestuff method, it is characterised in that step 1), 2) described in
High-pressure pulse electric electric field intensity is 30-40kV/cm, and umber of pulse is 8-10, step 1) extraction in high-pressure pulse electric time
Between 0.5-1 hour, step 2) extraction time in high-pressure pulse electric is 1-1.5 hour.
The most according to claim 1 based on Radix seu Folium Alchorneae trewioidis extract dyestuff method, it is characterised in that step 2) in ethanol molten
Liquid is the ethanol water of mass fraction 80-90%.
The most according to claim 1 based on Radix seu Folium Alchorneae trewioidis extract dyestuff method, it is characterised in that step 4) in distilled water with
The weight ratio of extracting solution is 1: 10, ethanol solution and the weight ratio 1: 5 of extracting solution.
The most according to claim 1 based on Radix seu Folium Alchorneae trewioidis extract dyestuff method, it is characterised in that step 4) described in ethanol
Solution is mass fraction 50-60% ethanol water.
The most according to claim 1 based on Radix seu Folium Alchorneae trewioidis extract dyestuff method, it is characterised in that step 4) described in big
Macroporous adsorbent resin is the one in AB-8, S-B, NKA, X-5, D296R.
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| CN201610559048.5A CN106189349A (en) | 2016-07-15 | 2016-07-15 | A kind of method extracting dyestuff based on Radix seu Folium Alchorneae trewioidis |
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| CN201610559048.5A CN106189349A (en) | 2016-07-15 | 2016-07-15 | A kind of method extracting dyestuff based on Radix seu Folium Alchorneae trewioidis |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108112866A (en) * | 2017-12-27 | 2018-06-05 | 福建省农业科学院亚热带农业研究所 | The acquisition methods of anthocyanidin sterling in a kind of gynura bicolor blade |
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| CN1896146A (en) * | 2005-07-15 | 2007-01-17 | 唐肖洪 | Extraction of natural edible amaranth pigment |
| CN101659793A (en) * | 2009-09-18 | 2010-03-03 | 华南师范大学 | Method for extracting gynura bicolor purpurin |
| CN101828764A (en) * | 2010-05-12 | 2010-09-15 | 广州秘理普植物技术开发有限公司 | Gynura bicolor extract and usage thereof in removing free radical in cigarette smoke |
| CN102911511A (en) * | 2011-08-02 | 2013-02-06 | 苏州宝泽堂医药科技有限公司 | Method for extracting purple pigment and total flavonoids from fresh gynura bicolor |
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|---|---|---|---|---|
| CN1896146A (en) * | 2005-07-15 | 2007-01-17 | 唐肖洪 | Extraction of natural edible amaranth pigment |
| CN101659793A (en) * | 2009-09-18 | 2010-03-03 | 华南师范大学 | Method for extracting gynura bicolor purpurin |
| CN101828764A (en) * | 2010-05-12 | 2010-09-15 | 广州秘理普植物技术开发有限公司 | Gynura bicolor extract and usage thereof in removing free radical in cigarette smoke |
| CN102911511A (en) * | 2011-08-02 | 2013-02-06 | 苏州宝泽堂医药科技有限公司 | Method for extracting purple pigment and total flavonoids from fresh gynura bicolor |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108112866A (en) * | 2017-12-27 | 2018-06-05 | 福建省农业科学院亚热带农业研究所 | The acquisition methods of anthocyanidin sterling in a kind of gynura bicolor blade |
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