CN102911511A - Method for extracting purple pigment and total flavonoids from fresh gynura bicolor - Google Patents
Method for extracting purple pigment and total flavonoids from fresh gynura bicolor Download PDFInfo
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- CN102911511A CN102911511A CN2011102186853A CN201110218685A CN102911511A CN 102911511 A CN102911511 A CN 102911511A CN 2011102186853 A CN2011102186853 A CN 2011102186853A CN 201110218685 A CN201110218685 A CN 201110218685A CN 102911511 A CN102911511 A CN 102911511A
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- purpurin
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- total flavones
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- phoenix dish
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Abstract
The invention belongs to the technical field of biology, and discloses a method for extracting a purple pigment and total flavonoids from fresh gynura bicolor. The method is characterized in that: (1) fresh gynura bicolor is crushed; 2-4 time amounts of 70-90% of ethanol is added for warm-soaking extraction 2-3 times; the extraction liquid is filtered and is subjected to ultrafiltration in an ultrafiltration membrane system; a permeate is concentrated in a nano-filtration membrane system; a concentrate is subjected to reduced-pressure concentration until no alcohol is left; the concentrate is absorbed by using ion exchange resin; water is used for washing until no sugar color is left; 3-5 BV of a 50-70% ethanol solution is used for eluting; an elution liquid is collected and is subjected to reduced-pressure concentration; and a concentrate is subjected to vacuum drying, such that the purple pigment is obtained; (2) off-column liquid of the ion column and the washing liquid are absorbed by using macroporous resin; 4-6 BV of a 40-60% ethanol solution is used for eluting; an elution liquid is subjected to reduced-pressure concentration; the concentrate is subjected to standing crystallization; and the product is filtered and dried, such that total flavonoids are obtained. The method provided by the invention has the advantages of simple process, easy operation, low energy consumption during the production process, low pollution, high raw material utilization rate, and suitability for industrialized productions.
Description
Technical field
The invention belongs to biological technical field, especially relate to a kind of method of from new scarlet phoenix dish, extracting purpurin and total flavones.
Background technology
Red phoenix dish is feverfew, contains abundant mineral substance, vitamins C and amino acid etc., is the traditional hill vegetables of China, and its herb can be adopted the whole year, using fresh herb or dry edible all can, a kind of advantageous green food and dietary supplements are favored by the human consumer deeply.Its flavor is hot, sweet, cool in nature, has clearing heat and cooling blood, invigorates blood circulation, hemostasis, detoxify and promote the subsdence of swelling effect.Being usually used in treating hemoptysis, uterine bleeding, traumatic hemorrhage, dysmenorrhoea, dysentery, sore swollen toxin, wound, ulcer does not restrain for a long time yet.
Modern study shows, contains a large amount of purpurins in the red phoenix dish, has anti-oxidant, effect of scavenging radical, reduces low-density lipoprotein (LDL), and anti-mutation, antitumor improves the eyesight effect.Contain in addition Flavonoid substances.
The method of extracting purpurin and flavones from red phoenix dish has:
Patent (application number CN200910192491) " a kind of extracting method of gynura bicolor purpurin ", the method for this patent disclosure be with water as extraction agent, and in conjunction with purification with macroreticular resin, from red phoenix dish, extract and obtain natural purpurin.
Patent (application number CN201010611189) " a kind of preparation method of red phoenix dish extractive of general flavone ", the method for this patent disclosure is: (1) is with the dry pulverizing of red phoenix dried vegetable; (2) with ethanol or methanol extraction, united extraction liquid, concentrating under reduced pressure becomes medicinal extract; (3) medicinal extract that step (2) is obtained adds water and places cold sinking, the centrifugal precipitation of going, and supernatant liquor is for subsequent use; (4) macroporous adsorbent resin on the supernatant liquor that step (3) is obtained is used ethanol gradient elution; (5) collect step (4) ethanol eluate concentrating under reduced pressure, go up again macroporous resin, use ethanol gradient elution; (6) step (5) ethanol eluate concentrate drying gets red phoenix dish extractive of general flavone.
And from red phoenix dish, extract simultaneously simultaneously the method for these two kinds of compositions, have no through retrieval.Usedly provide a kind of and extract two kinds of compositions, methods from red phoenix dish and be necessary.
Summary of the invention
A kind of method of from new scarlet phoenix dish, extracting purpurin and total flavones of purpose of the present invention.
The objective of the invention is to be achieved through the following technical solutions:
A kind of method of extracting purpurin and total flavones from new scarlet phoenix dish is characterized in that:
1) new scarlet phoenix dish is pulverized, adding the lixiviate of 2-4 times of 70-90% ethanol temperature gets 2-3 time, extracting solution filters and adds ultrafiltration in the ultrafiltration membrane system, seeing through liquid adds in the nanofiltration membrane system concentrated again, concentrated solution is evaporated to without adsorbing in the alcohol adding ion exchange resin, and washing is got 3-5BV50-70% ethanolic soln wash-out without fried sugar, collect the elutriant concentrating under reduced pressure, concentrated solution vacuum-drying namely gets purpurin;
2) adsorb in above-mentioned ion column lower column liquid and the water lotion adding macroporous resin adsorption, get 4-6BV40-60% ethanolic soln wash-out, the elutriant concentrating under reduced pressure, concentrated solution is placed crystallization, leaches and is drying to obtain total flavones.
Step 1) ultra-filtration membrane is the hollow cellulose ultra-filtration membrane of the 3000-10000 of molecular weight cut-off in.
Step 1) nanofiltration membrane is that molecular weight cut-off is the hollow cellulose nanofiltration membrane of 200-300 in.
Step 1) the optional D311 of intermediate ion exchange resin model, 330 and D380 in a kind of.
Step 2) a kind of among the optional HZ816 of macroporous resin model, HPD400 and the AB-8.
Technical superiority of the present invention is: method is extracted two kinds of compositions simultaneously from red phoenix dish, not only improved raw material availability, but also it is high to improve products obtained therefrom purity; Method adopts membrane filtration to belong to remove impurity make use of physics methods, and not only energy consumption is low, pollution is little, can also reduce the Yin Gaowen pigment and decompose, and improves yield.
Further specify the present invention below in conjunction with embodiment, but the scope of protection of present invention is not limited to the following example.
Embodiment
Embodiment 1:
New scarlet phoenix dish is pulverized, take by weighing 20kg add 4 times of 70% ethanol be heated to 50 ℃ the insulation 1 hour, extract 2 times, united extraction liquid filter to add ultrafiltration in the hollow cellulose film system of molecular weight cut-off 3000, sees through liquid and adds nanofiltration in 300 the hollow cellulose film system again, and concentrated solution is evaporated to add in the 2LD311 ion exchange resin without alcohol again and adsorbs, washing is without fried sugar, get 5BV50% ethanolic soln wash-out, collect the elutriant concentrating under reduced pressure, concentrated solution vacuum-drying namely gets purpurin 23g.
Adsorbing in ion column lower column liquid and the water lotion merging adding 1LHZ816 macroporous resin, get 4BV60% ethanolic soln wash-out, the elutriant concentrating under reduced pressure, concentrated solution is placed crystallization, leaches and is drying to obtain total flavones 35g.
Embodiment 2:
New scarlet phoenix dish is pulverized, take by weighing 20kg add 3 times of 90% ethanol be heated to 45 ℃ the insulation 1 hour, extract 3 times, united extraction liquid filter to add ultrafiltration in the hollow cellulose film system of molecular weight cut-off 10000, sees through liquid and adds nanofiltration in 200 the hollow cellulose film system again, and concentrated solution is evaporated to add in the 2.5L330 ion exchange resin without alcohol again and adsorbs, washing is without fried sugar, get 3BV70% ethanolic soln wash-out, collect the elutriant concentrating under reduced pressure, concentrated solution vacuum-drying namely gets purpurin 28g.
Adsorbing in ion column lower column liquid and the water lotion merging adding 1.5LHPD400 macroporous resin, get 5BV50% ethanolic soln wash-out, the elutriant concentrating under reduced pressure, concentrated solution is placed crystallization, leaches and is drying to obtain total flavones 42g.
Embodiment 3:
New scarlet phoenix dish is pulverized, take by weighing 20kg add 2 times of 80% ethanol be heated to 55 ℃ the insulation 1 hour, extract 3 times, united extraction liquid filter to add ultrafiltration in the hollow cellulose film system of molecular weight cut-off 6000, sees through liquid and adds nanofiltration in 300 the hollow cellulose film system again, and concentrated solution is evaporated to add in the 2LD380 ion exchange resin without alcohol again and adsorbs, wash colourless, get 4BV60% ethanolic soln wash-out, collect the elutriant concentrating under reduced pressure, concentrated solution vacuum-drying namely gets purpurin 35g.
Adsorbing in ion column lower column liquid and the water lotion merging adding 2LAB-8 macroporous resin, get 5BV40% ethanolic soln wash-out, the elutriant concentrating under reduced pressure, concentrated solution is placed crystallization, leaches and is drying to obtain total flavones 38g.
Claims (5)
1. method of extracting purpurin and total flavones from new scarlet phoenix dish is characterized in that:
1) new scarlet phoenix dish is pulverized, adding the lixiviate of 2-4 times of 70-90% ethanol temperature gets 2-3 time, extracting solution filters and adds ultrafiltration in the ultrafiltration membrane system, seeing through liquid adds in the nanofiltration membrane system concentrated again, concentrated solution is evaporated to without adsorbing in the alcohol adding ion exchange resin, and washing is got 3-5BV50-70% ethanolic soln wash-out without fried sugar, collect wash-out fluidity concentrating under reduced pressure, concentrated solution vacuum-drying namely gets purpurin;
2) adsorb in above-mentioned ion column lower column liquid and the water lotion adding macroporous resin, get 4-6BV40-60% ethanolic soln wash-out, the elutriant concentrating under reduced pressure, concentrated solution is placed crystallization, leaches and is drying to obtain total flavones.
2. from new scarlet phoenix dish, extract as claimed in claim 1 the method for purpurin and total flavones, it is characterized in that described step 1) in ultra-filtration membrane be the hollow cellulose ultra-filtration membrane of the 3000-10000 of molecular weight cut-off.
3. from new scarlet phoenix dish, extract as claimed in claim 1 the method for purpurin and total flavones, it is characterized in that described step 1) in nanofiltration membrane be that molecular weight cut-off is the hollow cellulose nanofiltration membrane of 200-300.
4. from new scarlet phoenix dish, extract as claimed in claim 1 the method for purpurin and total flavones, it is characterized in that described step 1) the optional D311 of intermediate ion exchange resin model, 330 and D380 in a kind of.
5. from new scarlet phoenix dish, extract as claimed in claim 1 the method for purpurin and total flavones, it is characterized in that described step 2) in a kind of among the optional HZ816 of macroporous resin model, HPD400 and the AB-8.
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| CN2011102186853A CN102911511A (en) | 2011-08-02 | 2011-08-02 | Method for extracting purple pigment and total flavonoids from fresh gynura bicolor |
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| CN2011102186853A CN102911511A (en) | 2011-08-02 | 2011-08-02 | Method for extracting purple pigment and total flavonoids from fresh gynura bicolor |
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| CN102911511A true CN102911511A (en) | 2013-02-06 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106189349A (en) * | 2016-07-15 | 2016-12-07 | 广西顺帆投资有限公司 | A kind of method extracting dyestuff based on Radix seu Folium Alchorneae trewioidis |
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| CN1517343A (en) * | 2003-01-13 | 2004-08-04 | 大兴安岭华野生物工程有限公司 | Extraction method of huckleberry anthocyanidin |
| JP2005194449A (en) * | 2004-01-09 | 2005-07-21 | Ishikawa Pref Gov | Method for producing dried kinji herb, and method for producing pigment using the same |
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| CN101045741A (en) * | 2007-04-27 | 2007-10-03 | 浙江工商大学 | Method for separating preparing anthocyan monomer from mulberry |
| JP2007289057A (en) * | 2006-04-25 | 2007-11-08 | Ishikawa Pref Gov | Method for processing kinji-sou, extract comprising kinji-sou active ingredients, and product containing the extract |
| CN101659793A (en) * | 2009-09-18 | 2010-03-03 | 华南师范大学 | Method for extracting gynura bicolor purpurin |
| CN102078357A (en) * | 2010-12-29 | 2011-06-01 | 江苏省中国科学院植物研究所 | Preparation method of Gynura bicolor total flavone extract |
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2011
- 2011-08-02 CN CN2011102186853A patent/CN102911511A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1517343A (en) * | 2003-01-13 | 2004-08-04 | 大兴安岭华野生物工程有限公司 | Extraction method of huckleberry anthocyanidin |
| JP2005194449A (en) * | 2004-01-09 | 2005-07-21 | Ishikawa Pref Gov | Method for producing dried kinji herb, and method for producing pigment using the same |
| CN1896146A (en) * | 2005-07-15 | 2007-01-17 | 唐肖洪 | Extraction of natural edible amaranth pigment |
| JP2007289057A (en) * | 2006-04-25 | 2007-11-08 | Ishikawa Pref Gov | Method for processing kinji-sou, extract comprising kinji-sou active ingredients, and product containing the extract |
| CN101045741A (en) * | 2007-04-27 | 2007-10-03 | 浙江工商大学 | Method for separating preparing anthocyan monomer from mulberry |
| CN101659793A (en) * | 2009-09-18 | 2010-03-03 | 华南师范大学 | Method for extracting gynura bicolor purpurin |
| CN102078357A (en) * | 2010-12-29 | 2011-06-01 | 江苏省中国科学院植物研究所 | Preparation method of Gynura bicolor total flavone extract |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106189349A (en) * | 2016-07-15 | 2016-12-07 | 广西顺帆投资有限公司 | A kind of method extracting dyestuff based on Radix seu Folium Alchorneae trewioidis |
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Application publication date: 20130206 |