CN1517343A - Extraction method of huckleberry anthocyanidin - Google Patents
Extraction method of huckleberry anthocyanidin Download PDFInfo
- Publication number
- CN1517343A CN1517343A CNA031108709A CN03110870A CN1517343A CN 1517343 A CN1517343 A CN 1517343A CN A031108709 A CNA031108709 A CN A031108709A CN 03110870 A CN03110870 A CN 03110870A CN 1517343 A CN1517343 A CN 1517343A
- Authority
- CN
- China
- Prior art keywords
- resin
- extracting method
- wash
- stripping liquid
- bilberry
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
A process for extracting the anthocyanidin from cowberry blueberry includes such steps as mixing the filtrate, evaporation concentrating to remove alcohol, macroreticular resin adsorbing, flushing the column with-clean water, eduting the resin with 5-55% alcohol solution, concentrating the desorptive liquid for removing alcohol, diluting with water, and ionic resin adsorption. Its advantages are simple process, high purity up to 35% and low cost.
Description
Technical field: the present invention relates to the improvement of bilberry anthocyanosides extracting method.
Background technology: the extraction of anthocyanidin is the in the majority of raw material with Vaccinium myrtillus L., Lonicera edulis Turcz. etc. at present, the extraction process of anthocyanidin uses conventional methods mostly---solvent extration, the shortcoming of this method is that extract purity is low, solvent cost is but very high, recently also useful Choice of Resin absorption method is extracted anthocyanidin, but the anthocyanidin effective component content that obtains has only 15%, also differs certain distance apart from international standard 25%.
Summary of the invention; For solving the problem that prior art exists, the invention provides a kind of extracting method of bilberry anthocyanosides, the high characteristics of anthocyanidin purity that it should have, and technology is simple, cost is low, extract.The present invention is achieved in that and gets the broken squeezing of bilberry centrifugation, and fruit juice is stand-by after filtering, and broken pericarp fruit seed leaches immersion liquid with 85% alcohol immersion 4 hours, use the same method the again immersion and filtering twice of last broken pericarp fruit seed.Filtrate is mixed into thickner steams ethanol, mix with the fruit juice after filtering, last macroporous resin adsorption post is adsorbed to saturated, colourless with water purification flushing adsorption column to anacidity, ethanol elution resin with 5%～55% concentration gets stripping liquid again, stripping liquid is concentrated steaming fall ethanol, adsorb through ion exchange resin again behind the dilute with water, adsorb saturated back and wash the ion exchange resin adsorption column with water purification, ethanol elution resin with 5%～55% concentration gets stripping liquid one time again, repeat the absorption of macroporous resin and ion exchange resin in the same way, wash-out 3～5 times concentrates stripping liquid at last, and is spray-dried that dry powder is bilberry anthocyanosides.The temperature of stripping liquid is controlled at 30～40 ℃ in present method, and spray-dired temperature is controlled at 180 ℃ and 80 ℃.The present invention adopts the resourceful Vaccinium uliginosum fruit in the big Xiaoxinanlin Mountains, by the macroporous resin adsorption post bilberry anthocyanosides is absorbed earlier, adsorb to fall non-bilberry anthocyanosides composition through ion exchange resin again, the anthocyanidin purity of extracting can reach 35%, surpass 10 percentage points of international standards, it is simple to have technology, and cost is low, the high advantage of anthocyanidin purity of extraction.
Embodiment one: present embodiment is achieved in that gets the broken squeezing of bilberry centrifugation, fruit juice is stand-by after filtering, broken pericarp fruit seed leaches immersion liquid with 85% alcohol immersion 4 hours, use the same method the again immersion and filtering twice of last broken pericarp fruit seed.Filtrate is mixed into thickner steams ethanol, mix with the fruit juice after filtering, last macroporous resin adsorption post is adsorbed to saturated, colourless with water purification flushing adsorption column to anacidity, ethanol elution resin with 5%～55% concentration gets stripping liquid again, stripping liquid is concentrated steaming fall ethanol, adsorb through ion exchange resin again behind the dilute with water, adsorb saturated back and wash the ion exchange resin adsorption column with water purification, ethanol elution resin with 5%～55% concentration gets stripping liquid one time, repeat the absorption of macroporous resin and ion exchange resin in the same way, wash-out 3～5 times concentrates stripping liquid at last, and is spray-dried that dry powder is bilberry anthocyanosides.The temperature of stripping liquid is controlled at 30～40 ℃ in the present embodiment, and spray-dired temperature is controlled at 180 ℃ and 80 ℃.The model of macroporous resin is HPD100 in the present embodiment, and the model of ion exchange resin is S
3
Embodiment two: the alcohol concn of wash-out resin is 5%～15% in the present embodiment.
Embodiment three: the alcohol concn of wash-out resin is 16%～25% in the present embodiment.
Embodiment four: the alcohol concn of wash-out resin is 26%～35% in the present embodiment.
Embodiment five: the alcohol concn of wash-out resin is 36%～45% in the present embodiment.
Embodiment six: the alcohol concn of wash-out resin is 46%～55% in the present embodiment.
Embodiment seven: the temperature of stripping liquid is controlled at 30～35 ℃ in the present embodiment.
Embodiment eight: the temperature of stripping liquid is controlled at 36～40 ℃ in the present embodiment.
Embodiment nine: the optimum temps of stripping liquid is controlled at 35 ℃ in the present embodiment.
Claims (9)
1, the extracting method of bilberry anthocyanosides, it is characterized in that getting the broken squeezing of bilberry centrifugation, fruit juice is stand-by after filtering, and broken pericarp fruit seed was with 85% alcohol immersion 4 hours, leach immersion liquid, last broken pericarp fruit seed uses the same method and soaks and filter twice.Filtrate is mixed into thickner steams ethanol, mix with the fruit juice after filtering, last macroporous resin adsorption post is adsorbed to saturated, colourless with water purification flushing adsorption column to anacidity, ethanol elution resin with 5%～55% concentration gets stripping liquid again, stripping liquid is concentrated steaming fall ethanol, adsorb through ion exchange resin again behind the dilute with water, adsorb saturated back and wash the ion exchange resin adsorption column with water purification, ethanol elution resin with 5%～55% concentration gets stripping liquid one time, repeat the absorption of macroporous resin and ion exchange resin in the same way, wash-out 3～5 times concentrates stripping liquid at last, and is spray-dried that dry powder is bilberry anthocyanosides, the temperature of stripping liquid is controlled at 30～40 ℃, spray-dired temperature is controlled at 180 ℃ and 80 ℃, and the model of macroporous resin is HPD100, and the model of ion exchange resin is S
3
2, the extracting method of bilberry anthocyanosides according to claim 1, the alcohol concn that it is characterized in that the wash-out resin is 5%～15%.
3, the extracting method of bilberry anthocyanosides according to claim 1, the alcohol concn that it is characterized in that the wash-out resin is 16%～25%.
4, the extracting method of bilberry anthocyanosides according to claim 1, the alcohol concn that it is characterized in that the wash-out resin is 26%～35%.
5, the extracting method of bilberry anthocyanosides according to claim 1, the alcohol concn that it is characterized in that the wash-out resin is 36%～45%.
6, the extracting method of bilberry anthocyanosides according to claim 1, the alcohol concn that it is characterized in that the wash-out resin is 46%～55%.
7, the extracting method of bilberry anthocyanosides according to claim 1 is characterized in that the temperature of stripping liquid is controlled at 30～35 ℃.
8, the extracting method of bilberry anthocyanosides according to claim 1 is characterized in that the temperature of stripping liquid is controlled at 36～40 ℃.
9, the extracting method of bilberry anthocyanosides according to claim 1 is characterized in that the optimum temps of stripping liquid is controlled at 35 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA031108709A CN1517343A (en) | 2003-01-13 | 2003-01-13 | Extraction method of huckleberry anthocyanidin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA031108709A CN1517343A (en) | 2003-01-13 | 2003-01-13 | Extraction method of huckleberry anthocyanidin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1517343A true CN1517343A (en) | 2004-08-04 |
Family
ID=34283284
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA031108709A Pending CN1517343A (en) | 2003-01-13 | 2003-01-13 | Extraction method of huckleberry anthocyanidin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1517343A (en) |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101215424B (en) * | 2008-01-16 | 2010-06-02 | 华东师范大学 | Method for extracting and refining bilberry schlempe anthocyanidin |
| CN101265252B (en) * | 2008-04-28 | 2011-03-23 | 吉林市新科奇保健食品有限公司 | Vaccinium uliginosum cyanidin and separation and purification method thereof |
| CN101525327B (en) * | 2008-03-06 | 2011-12-14 | 陕西嘉禾植物化工有限责任公司 | Method for extracting anthocyanin from cowberry |
| CN101531652B (en) * | 2009-04-17 | 2012-05-16 | 大连理工大学 | Novel method for extracting anthocyanin in bilberry by double water phases |
| CN102911511A (en) * | 2011-08-02 | 2013-02-06 | 苏州宝泽堂医药科技有限公司 | Method for extracting purple pigment and total flavonoids from fresh gynura bicolor |
| CN103130762A (en) * | 2013-01-31 | 2013-06-05 | 宁波中药制药有限公司 | Method for extracting anthocyanin from indigo indicum |
| CN104431998A (en) * | 2013-09-16 | 2015-03-25 | 中国科学院城市环境研究所 | An anthocyanin health product for antioxidation |
| CN105061530A (en) * | 2015-08-31 | 2015-11-18 | 桂林茗兴生物科技有限公司 | Technology for extracting waxberry anthocyanin |
| CN105153253A (en) * | 2015-08-31 | 2015-12-16 | 桂林茗兴生物科技有限公司 | Purple sweet potato anthocyanin extraction process |
| CN105175463A (en) * | 2015-08-31 | 2015-12-23 | 桂林茗兴生物科技有限公司 | Extraction process for blueberry anthocyanin |
| CN105418572A (en) * | 2015-11-06 | 2016-03-23 | 大兴安岭林格贝寒带生物科技股份有限公司 | Method for extracting whortleberry anthocyanidin in microwave countercurrent mode and adsorbing and purifying whortleberry anthocyanidin through macroporous adsorbent resin |
| CN106214731A (en) * | 2016-08-29 | 2016-12-14 | 盛林蓝莓集团股份有限公司 | Blueberry raw anthocyanidin biological extraction process |
| CN107281252A (en) * | 2017-06-30 | 2017-10-24 | 杨凌萃健生物工程技术有限公司 | A kind of preparation method of whortle extract |
| CN107440117A (en) * | 2017-08-04 | 2017-12-08 | 浙江绍兴东灵保健食品有限公司 | A kind of Bilberry fruit P.E production technology |
| US9969707B2 (en) | 2011-12-16 | 2018-05-15 | CENTRE DE RECHERCHE INDUSTRIELLE DU QUéBEC | Method for extracting anthocyanin derivatives from a plant source |
| CN113350227A (en) * | 2021-06-07 | 2021-09-07 | 上海其然生物科技有限公司 | Cleansing oil containing oil-soluble and water-soluble vaccinium myrtillus active ingredients and preparation method thereof |
-
2003
- 2003-01-13 CN CNA031108709A patent/CN1517343A/en active Pending
Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101215424B (en) * | 2008-01-16 | 2010-06-02 | 华东师范大学 | Method for extracting and refining bilberry schlempe anthocyanidin |
| CN101525327B (en) * | 2008-03-06 | 2011-12-14 | 陕西嘉禾植物化工有限责任公司 | Method for extracting anthocyanin from cowberry |
| CN101265252B (en) * | 2008-04-28 | 2011-03-23 | 吉林市新科奇保健食品有限公司 | Vaccinium uliginosum cyanidin and separation and purification method thereof |
| CN101531652B (en) * | 2009-04-17 | 2012-05-16 | 大连理工大学 | Novel method for extracting anthocyanin in bilberry by double water phases |
| CN102911511A (en) * | 2011-08-02 | 2013-02-06 | 苏州宝泽堂医药科技有限公司 | Method for extracting purple pigment and total flavonoids from fresh gynura bicolor |
| US9969707B2 (en) | 2011-12-16 | 2018-05-15 | CENTRE DE RECHERCHE INDUSTRIELLE DU QUéBEC | Method for extracting anthocyanin derivatives from a plant source |
| CN103130762A (en) * | 2013-01-31 | 2013-06-05 | 宁波中药制药有限公司 | Method for extracting anthocyanin from indigo indicum |
| CN103130762B (en) * | 2013-01-31 | 2016-08-03 | 宁波中药制药股份有限公司 | A kind of method extracting anthocyanidin from indigo fruit |
| CN104431998A (en) * | 2013-09-16 | 2015-03-25 | 中国科学院城市环境研究所 | An anthocyanin health product for antioxidation |
| CN105153253A (en) * | 2015-08-31 | 2015-12-16 | 桂林茗兴生物科技有限公司 | Purple sweet potato anthocyanin extraction process |
| CN105175463A (en) * | 2015-08-31 | 2015-12-23 | 桂林茗兴生物科技有限公司 | Extraction process for blueberry anthocyanin |
| CN105061530A (en) * | 2015-08-31 | 2015-11-18 | 桂林茗兴生物科技有限公司 | Technology for extracting waxberry anthocyanin |
| CN105153253B (en) * | 2015-08-31 | 2018-07-20 | 桂林茗兴生物科技有限公司 | The extraction process of anthocyanins pigment from purple sweet potato |
| CN105061530B (en) * | 2015-08-31 | 2018-07-20 | 桂林茗兴生物科技有限公司 | The extraction process of red bayberry anthocyanin |
| CN105175463B (en) * | 2015-08-31 | 2018-07-17 | 桂林茗兴生物科技有限公司 | The extraction process of Anthocyanin from Blueberry |
| CN105418572A (en) * | 2015-11-06 | 2016-03-23 | 大兴安岭林格贝寒带生物科技股份有限公司 | Method for extracting whortleberry anthocyanidin in microwave countercurrent mode and adsorbing and purifying whortleberry anthocyanidin through macroporous adsorbent resin |
| CN105418572B (en) * | 2015-11-06 | 2019-02-12 | 大兴安岭林格贝寒带生物科技股份有限公司 | It is extracted using microwave counter current, the method for macroporous absorbent resin adsorption and purification whortle anthocyanidin |
| CN106214731A (en) * | 2016-08-29 | 2016-12-14 | 盛林蓝莓集团股份有限公司 | Blueberry raw anthocyanidin biological extraction process |
| CN107281252A (en) * | 2017-06-30 | 2017-10-24 | 杨凌萃健生物工程技术有限公司 | A kind of preparation method of whortle extract |
| CN107281252B (en) * | 2017-06-30 | 2020-05-22 | 杨凌萃健生物工程技术有限公司 | Preparation method of vaccinium myrtillus extract |
| CN107440117A (en) * | 2017-08-04 | 2017-12-08 | 浙江绍兴东灵保健食品有限公司 | A kind of Bilberry fruit P.E production technology |
| CN113350227A (en) * | 2021-06-07 | 2021-09-07 | 上海其然生物科技有限公司 | Cleansing oil containing oil-soluble and water-soluble vaccinium myrtillus active ingredients and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1517343A (en) | Extraction method of huckleberry anthocyanidin | |
| CN105193863B (en) | A kind of preparation method of high-purity seeweed polyphenol | |
| CN103467540B (en) | A kind of method extracting rhodioside from Root of Kirilow Rhodiola | |
| CN109293712B (en) | Industrialized utilization method of stevia rebaudiana and stevioside and chlorogenic acid thereof | |
| CN102614834B (en) | Method for adsorbing and recovering heavy metals of lead and copper by persimmon tannin-containing metal adsorbent | |
| CN103497106A (en) | Method for extracting chlorogenic acid from green coffee beans | |
| CN102432582A (en) | Preparation method of proanthocyanidin | |
| CN102285953A (en) | Method for separating and purifying blueberry anthocyanidin by using HP2MGL macroporous resin | |
| CN103494862B (en) | Method for extracting olive polyphenol from olive processing waste liquor | |
| CN103012544B (en) | A kind of method extracting saponin and polysaccharide from tea seed grouts | |
| CN105053952B (en) | A kind of processing technology of the dried orange peel extracts of no bitter taste | |
| CN106565795A (en) | Method for extracting alkaloid from bitter gourds | |
| CN104119229A (en) | Technology for producing pure chlorogenic acid | |
| CN109021042B (en) | Method for extracting high-purity oleuropein from olive leaves | |
| CN102516805A (en) | Method for extracting anthocyanin from red rice red | |
| CN101987856B (en) | Preparation method of anthocyanin monomer cornflower-3-glucoside | |
| CN104945450A (en) | Method for extracting stibene glucoside from vines of multiflower knotweeds | |
| CN102293791A (en) | Production method for extracting effective components of gingko leaf | |
| CN106397529B (en) | The method of extraction separation crataegolic acid from olive pomace | |
| CN1008821B (en) | Making method of natural blue total saponin | |
| CN100362011C (en) | Preparation method of ginkgo leaf extract | |
| CN104230871A (en) | Method for separating polymethoxylated flavones, hesperidin and synephrine from immature bitter orange | |
| CN1281600C (en) | Method for extracting high purity seabuckthorn flavone aglycone | |
| CN106336440A (en) | Method for extracting and separating out oleanolic acid from olive leaves | |
| CN1147455C (en) | Green extraction process of high-purity tea polyphenol |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |