CN101703554A - Preparation and use of semen cuscutae flavonoids - Google Patents

Preparation and use of semen cuscutae flavonoids Download PDF

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Publication number
CN101703554A
CN101703554A CN200910230096A CN200910230096A CN101703554A CN 101703554 A CN101703554 A CN 101703554A CN 200910230096 A CN200910230096 A CN 200910230096A CN 200910230096 A CN200910230096 A CN 200910230096A CN 101703554 A CN101703554 A CN 101703554A
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semen cuscutae
ethanol
total flavones
preparation
extract
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CN200910230096A
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CN101703554B (en
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段洪东
张屹
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Shandong Institute of Light Industry
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段洪东
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Abstract

The invention belongs to the technical field of medicaments and relates to the preparation and use of semen cuscutae flavonoids. The semen cuscutae flavonoids are prepared by an alcohol reflux extraction method. The semen cuscutae flavonoids have effects of nourishing the liver and the kidney, replenishing vital essence to improve eyesight, calming fetus and the like, are the important Chinese medicaments for nourishing kidney, strengthening Yang and arresting emission and have good development prospects.

Description

A kind of preparation of Semen Cuscutae total flavones and application
Technical field:
The present invention relates to Uigurs medicine composition purification process, relate in particular to its preparation of Semen Cuscutae total flavones extract and application, belong to medical technical field.
Background technology:
Semen Cuscutae is the mature seed of Convolvulaceae annual parasitics MANCAO Semen Cuscutae or cuscuta japonica Choisy.Have invigorating the liver and kidney, replenishing vital essence to improve eyesight, controlling nocturnal emission with astringent drugs reducing urination, the antiabortive effect of antidiarrheal.2000 editions the Pharmacopoeia of the People's Republic of China records it and is the dry mature seed of convolvulus cuscuta plant.Semen Cuscutae is recorded in Shennong's Herbal the earliest as a kind of YANG invigorating medicine commonly used, and is widely distributed in China place of production, main product Shandong, Hebei, Shanxi, Shaanxi, Jiangsu, Heilungkiang, Jilin, Xinjiang etc.What 29 provinces in the whole nation formed commodity is mainly derived from 4: Semen Cuscutae, southern Semen Cuscutae, European Semen Cuscutae, cuscuta japonica Choisy.
The chemical constituent of Semen Cuscutae mainly comprises chemical compounds such as flavonoid, polysaccharide, alkaloids, terpenoid, steroidal class, volatile oil and lignin.Wherein, content of total flavone is about 3.0%.Flavonoid is the main effective ingredient of Semen Cuscutae.Main flavone compound has kaempferol, Quercetin, hyperin, Herba Astragali Melilotoidis (Herba Astragali Sinici) glycosides etc.
The Semen Cuscutae place of production distributes wide, and output is big, is a kind of conventional Chinese medicine, property of medicine neutralization, and sweet hot tepor is gone into the Liver and kidney warp.Nourishing YIN Yiyang, Wen Erxiao is dry, benefit and little stagnate.Be the good medicine of flat invigorating the liver and kidney simply.Because the remarkable effect of Semen Cuscutae aspect kidney invigorating and YANG supporting, immunity, cardiovascular, find that in addition it is also having certain effect aspect antioxidation, the defying age, therefore become focus for the research of Semen Cuscutae in recent years, and to the research of Semen Cuscutae also mainly at aspects such as its process of preparing Chinese medicine, chemical constituent, pharmacology.Separation and purification for the Semen Cuscutae effective ingredient then awaits further research, to disclose its mechanism of action, is the clinical practice developing wide prospect of Semen Cuscutae.
Summary of the invention:
The weak point that exists on the extraction and purification method at total flavones in the existing Semen Cuscutae, the invention provides a kind of extraction and purification method and application thereof of Semen Cuscutae total flavones, the main ethanol reflux extraction that adopts, adopt this method, reduced the application of organic solvent in whole purification process, prevented the influence of organic solvent to end product, the prepared Semen Cuscutae total flavones extract of the inventive method has invigorating the liver and kidney, replenishing vital essence to improve eyesight, effect such as antiabortive, be the key medicine of traditional Chinese medical science the kidney invigorating, tonifying YANG, defecation essence, have bright development prospect.
Semen Cuscutae total flavones preparation method of extract of the present invention, its extraction purification step is:
1) gets dry Semen Cuscutae medical material, grind into powder; With ethanol is solvent, soaked 12 hours, be extractant with 95% ethanol of 6~8 times of amounts of quality of medicinal material, reflux, extract, 2~3 hours is collected extracting solution, decompression recycling ethanol is not to there being the alcohol flavor, concentrated solution;
2) extracting solution that step 1) is obtained is concentrated to every milliliter and contains total flavones 0.3g, filter, filtrate is carried out purification with HPD-100 or HPD-400 or AB-8 or D101 macroporous resin, last sample adsorption time is 0.1~2.5h, and the washing volume is 1BV~3BV, and the washing flow velocity is 1BV/h~5BV/h, eluant is an ethanol, the eluting concentration of alcohol is 30%~60%, and the ethanol elution amount is 2.5BV~4BV, and ethanol elution speed is 1BV/h~3BV/h.
3) ethanol elution collection step 2), vacuum drying must extract product.
Wherein preferred processing condition is in the step (2): HPD-400 or D101 macroporous resin carry out purification, and adsorption time is 0.5~1.0h, and volume fraction 40%~50% ethanol is eluant, and elution amount is 3BV, and elution speed is 2BV/h;
Use the Chinese medicine preparation of this Semen Cuscutae total flavones, it is characterized in that with described Semen Cuscutae total flavones be the medicine active ingredient, add tablet, the capsule made behind the pharmaceutic adjuvant.
Can be used for developing preparation invigorating the liver and kidney, replenishing vital essence to improve eyesight, antiabortive medicine.
The present invention has the following advantages:
1. Semen Cuscutae total flavones preparation method of extract provided by the invention is simple to operate, quick;
2. selected macroporous resin has the function that adsorbance is big, desorption efficiency is big;
3. with ethanol be solvent and eluant, inexpensive, nontoxic.
The specific embodiment:
Understand technical scheme of the present invention for better, below describe in further detail by specific embodiment.
Embodiment 1:
Get dry Semen Cuscutae medical material 100g, grind into powder; With ethanol is solvent, soaked 12 hours, be extractant with 95% ethanol of 6 times of amounts of quality of medicinal material, reflux, extract, 2 hours is collected extracting solution, decompression recycling ethanol is not to there being the alcohol flavor, concentrated solution; The extracting solution that step is obtained is concentrated to every milliliter and contains total flavones 0.3g, filter, filtrate is carried out purification with the HPD-100 macroporous resin, last sample adsorption time is 1.0h, and the washing volume is 3BV, and the washing flow velocity is 3BV/h, eluant is an ethanol, the eluting concentration of alcohol is 60%, and the ethanol elution amount is 4BV, and ethanol elution speed is 3BV/h.The ethanol elution of collecting, 50 ℃ of vacuum dryings get product.Yield is 2.91%.
Embodiment 2:
Get dry Semen Cuscutae medical material 100g, grind into powder; With ethanol is solvent, soaked 12 hours, be extractant with 95% ethanol of 8 times of amounts of quality of medicinal material, reflux, extract, 2 hours is collected extracting solution, decompression recycling ethanol is not to there being the alcohol flavor, concentrated solution; The extracting solution that step is obtained is concentrated to every milliliter and contains total flavones 0.3g, filter, filtrate is carried out purification with the HPD-400 macroporous resin, last sample adsorption time is 2.5h, and the washing volume is 3BV, and the washing flow velocity is 2BV/h, eluant is an ethanol, the eluting concentration of alcohol is 60%, and the ethanol elution amount is 3BV, and ethanol elution speed is 2BV/h.The ethanol elution of collecting, 50 ℃ of vacuum dryings get product.Yield is 3.12%.
Embodiment 3:
Get dry Semen Cuscutae medical material 100g, grind into powder; With ethanol is solvent, soaked 12 hours, be extractant with 95% ethanol of 8 times of amounts of quality of medicinal material, reflux, extract, 2 hours is collected extracting solution, decompression recycling ethanol is not to there being the alcohol flavor, concentrated solution; The extracting solution that step is obtained is concentrated to every milliliter and contains total flavones 0.3g, filter, filtrate is carried out purification with the D101 macroporous resin, last sample adsorption time is 2.5h, and the washing volume is 3BV, and the washing flow velocity is 3BV/h, eluant is an ethanol, the eluting concentration of alcohol is 40%, and the ethanol elution amount is 3BV, and ethanol elution speed is 3BV/h.The ethanol elution of collecting, 50 ℃ of vacuum dryings get product.Yield is 3.18%.
Embodiment 4 Semen Cuscutae total flavones sheets
Group component
Total flavones 1-100g
Sodium Hydroxymethyl Stalcs 25g
6% starch slurry 20ml
Magnesium stearate 3g
Preparation process: Semen Cuscutae total flavones, Sodium Hydroxymethyl Stalcs, magnesium stearate pulverize separately are crossed 80 mesh sieves; Semen Cuscutae total flavones, Sodium Hydroxymethyl Stalcs, 6% starch slurry are mixed, grind evenly, the system soft material; After 20 mesh sieve system granules, drying adds the magnesium stearate mixing again, presses 1000; Pack after the assay was approved.
Embodiment 5 Semen Cuscutae total flavones capsules
Group component
Semen Cuscutae total flavones 10-100g
Starch 50g
70% alcoholic solution 15ml
Preparation process: with starch and Semen Cuscutae total flavones mixing, cross 80 mesh sieves, add 70% alcoholic solution of 15ml, the system soft material, after 20 mesh sieve system granules, drying, granulate, encapsulated, make 1000 altogether, every contains Semen Cuscutae total flavones extract 10-100mg.

Claims (3)

1. Semen Cuscutae total flavones preparation method of extract is characterized in that: extracting purification step is:
1) gets dry Semen Cuscutae medical material, grind into powder; With ethanol is solvent, soaked 12 hours, be extractant with 95% ethanol of 6~8 times of amounts of quality of medicinal material, reflux, extract, 2~3 hours is collected extracting solution, decompression recycling ethanol is not to there being the alcohol flavor, concentrated solution;
2) extracting solution that step 1) is obtained is concentrated to every milliliter and contains total flavones 0.3g, filter, filtrate is carried out purification with HPD-100 or HPD-400 or AB-8 or D101 macroporous resin, last sample adsorption time is 0.1~2.5h, and the washing volume is 1BV~3BV, and the washing flow velocity is 1BV/h~5BV/h, eluant is an ethanol, the eluting concentration of alcohol is 30%~60%, and the ethanol elution amount is 2.5BV~4BV, and ethanol elution speed is 1BV/h~3BV/h.
3) ethanol elution collection step 2), vacuum drying must extract product.
2. Semen Cuscutae total flavones preparation method of extract according to claim 1, it is characterized in that: preferred processing condition is in the described step (2): HPD-400 or D101 macroporous resin carry out purification, adsorption time is 0.5~1.0h, volume fraction 40%~50% ethanol is eluant, elution amount is 3BV, and elution speed is 2BV/h
3. use the Chinese medicine preparation of Semen Cuscutae total flavones as claimed in claim 1, it is characterized in that with described Semen Cuscutae total flavones be the medicine active ingredient, add tablet, the capsule made behind the pharmaceutic adjuvant.
CN200910230096XA 2009-11-13 2009-11-13 Preparation and use of semen cuscutae flavonoids Expired - Fee Related CN101703554B (en)

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CN101703554B CN101703554B (en) 2011-10-05

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102846696A (en) * 2012-10-15 2013-01-02 江苏省中国科学院植物研究所 Traditional Chinese flavones extract for resisting ovarian function decline as well as preparation method and application thereof
CN103816204A (en) * 2013-11-19 2014-05-28 新乡医学院 Preparation method and clinical application of chemical modification of Chinese medicine semen cuscutae extract
CN108029727A (en) * 2018-01-15 2018-05-15 广州聚澜健康产业研究院有限公司 One kind smears tea multi-layer steamed bread and preparation method thereof
CN111358826A (en) * 2020-04-13 2020-07-03 宁夏医科大学 Total flavone extract with antidepressant effect, and preparation method and application thereof

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102846696A (en) * 2012-10-15 2013-01-02 江苏省中国科学院植物研究所 Traditional Chinese flavones extract for resisting ovarian function decline as well as preparation method and application thereof
CN102846696B (en) * 2012-10-15 2013-09-11 江苏省中国科学院植物研究所 Traditional Chinese flavones extract for resisting ovarian function decline as well as preparation method and application thereof
CN103816204A (en) * 2013-11-19 2014-05-28 新乡医学院 Preparation method and clinical application of chemical modification of Chinese medicine semen cuscutae extract
CN108029727A (en) * 2018-01-15 2018-05-15 广州聚澜健康产业研究院有限公司 One kind smears tea multi-layer steamed bread and preparation method thereof
CN111358826A (en) * 2020-04-13 2020-07-03 宁夏医科大学 Total flavone extract with antidepressant effect, and preparation method and application thereof
CN111358826B (en) * 2020-04-13 2022-03-15 宁夏医科大学 Total flavone extract with antidepressant effect, and preparation method and application thereof

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