CN106176882B - preparation method of radix pseudostellariae extract - Google Patents
preparation method of radix pseudostellariae extract Download PDFInfo
- Publication number
- CN106176882B CN106176882B CN201610759126.6A CN201610759126A CN106176882B CN 106176882 B CN106176882 B CN 106176882B CN 201610759126 A CN201610759126 A CN 201610759126A CN 106176882 B CN106176882 B CN 106176882B
- Authority
- CN
- China
- Prior art keywords
- filter
- radix pseudostellariae
- stage
- extraction
- check valve
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 238000000605 extraction Methods 0.000 claims abstract description 60
- 239000007788 liquid Substances 0.000 claims abstract description 51
- 238000004880 explosion Methods 0.000 claims abstract description 40
- 238000001914 filtration Methods 0.000 claims abstract description 33
- 238000000034 method Methods 0.000 claims abstract description 29
- 238000003860 storage Methods 0.000 claims abstract description 20
- 230000008569 process Effects 0.000 claims abstract description 14
- 238000003809 water extraction Methods 0.000 claims abstract description 12
- 238000001784 detoxification Methods 0.000 claims abstract description 7
- 230000000694 effects Effects 0.000 claims abstract description 6
- 238000005422 blasting Methods 0.000 claims description 46
- 239000012510 hollow fiber Substances 0.000 claims description 39
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 32
- 239000011229 interlayer Substances 0.000 claims description 30
- 230000005540 biological transmission Effects 0.000 claims description 29
- 239000011159 matrix material Substances 0.000 claims description 18
- 238000005057 refrigeration Methods 0.000 claims description 13
- 238000005119 centrifugation Methods 0.000 claims description 12
- 239000000706 filtrate Substances 0.000 claims description 12
- 238000004140 cleaning Methods 0.000 claims description 11
- 239000011347 resin Substances 0.000 claims description 10
- 229920005989 resin Polymers 0.000 claims description 10
- 229910010271 silicon carbide Inorganic materials 0.000 claims description 9
- 238000005520 cutting process Methods 0.000 claims description 7
- 101000623895 Bos taurus Mucin-15 Proteins 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 239000012535 impurity Substances 0.000 claims description 5
- 229910001220 stainless steel Inorganic materials 0.000 claims description 5
- 239000010935 stainless steel Substances 0.000 claims description 5
- 238000000926 separation method Methods 0.000 claims description 4
- 238000011001 backwashing Methods 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- 239000011148 porous material Substances 0.000 claims description 3
- 238000007781 pre-processing Methods 0.000 claims description 3
- 239000008213 purified water Substances 0.000 claims description 3
- 238000005516 engineering process Methods 0.000 abstract description 8
- 239000002994 raw material Substances 0.000 abstract description 8
- 102000004190 Enzymes Human genes 0.000 abstract description 7
- 108090000790 Enzymes Proteins 0.000 abstract description 7
- 238000001035 drying Methods 0.000 abstract description 7
- 231100000614 poison Toxicity 0.000 abstract description 5
- 239000003440 toxic substance Substances 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 244000005700 microbiome Species 0.000 abstract description 3
- 230000000415 inactivating effect Effects 0.000 abstract description 2
- 241000208340 Araliaceae Species 0.000 abstract 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 abstract 1
- 235000003140 Panax quinquefolius Nutrition 0.000 abstract 1
- 235000008434 ginseng Nutrition 0.000 abstract 1
- 241001038562 Pseudostellaria heterophylla Species 0.000 description 7
- 239000011575 calcium Substances 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 6
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 4
- 230000004888 barrier function Effects 0.000 description 4
- 230000009849 deactivation Effects 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 210000002421 cell wall Anatomy 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 241000411851 herbal medicine Species 0.000 description 3
- 229920005610 lignin Polymers 0.000 description 3
- 210000004072 lung Anatomy 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 210000000952 spleen Anatomy 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 2
- 102000001189 Cyclic Peptides Human genes 0.000 description 2
- 108010069514 Cyclic Peptides Proteins 0.000 description 2
- 229920002488 Hemicellulose Polymers 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 229910000019 calcium carbonate Inorganic materials 0.000 description 2
- 235000010216 calcium carbonate Nutrition 0.000 description 2
- 229910001424 calcium ion Inorganic materials 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- HYBBIBNJHNGZAN-UHFFFAOYSA-N furfural Chemical compound O=CC1=CC=CO1 HYBBIBNJHNGZAN-UHFFFAOYSA-N 0.000 description 2
- 229930182470 glycoside Natural products 0.000 description 2
- 150000002338 glycosides Chemical class 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 239000001814 pectin Substances 0.000 description 2
- 229920001277 pectin Polymers 0.000 description 2
- 235000010987 pectin Nutrition 0.000 description 2
- 230000000144 pharmacologic effect Effects 0.000 description 2
- 239000013049 sediment Substances 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- 206010002660 Anoxia Diseases 0.000 description 1
- 241000976983 Anoxia Species 0.000 description 1
- 241000219321 Caryophyllaceae Species 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 241001038563 Pseudostellaria Species 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 208000022531 anorexia Diseases 0.000 description 1
- 230000007953 anoxia Effects 0.000 description 1
- 206010003549 asthenia Diseases 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 206010061428 decreased appetite Diseases 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000000212 effect on lymphocytes Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000007087 memory ability Effects 0.000 description 1
- 230000002107 myocardial effect Effects 0.000 description 1
- 235000014593 oils and fats Nutrition 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/36—Caryophyllaceae (Pink family), e.g. babysbreath or soapwort
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Abstract
the invention belongs to the technical field of radix pseudostellariae extraction, and particularly discloses a preparation method of a radix pseudostellariae extract. Comprises six steps of radix pseudostellariae fresh ginseng pretreatment, two-stage steam explosion treatment, hot water extraction, extraction liquid CO2 treatment, three-stage filtration and detoxification. The two-stage steam explosion technology not only achieves the effect of extracting and pretreating the radix pseudostellariae, but also achieves the purpose of inactivating enzyme of fresh radix pseudostellariae, and meanwhile, the technology can reduce and even remove toxic substances generated in steam explosion; because the fresh radix pseudostellariae without fibrous roots is adopted as the raw material for extraction, the process of drying and processing the radix pseudostellariae can be omitted, the cost is obviously reduced, a large amount of time is saved, and the pollution of harmful microorganisms in the drying and storage process is reduced to the greatest extent; the preparation method is very suitable for the preparation and production of the radix pseudostellariae extract of the enterprises in the origin of radix pseudostellariae, and provides a new way for the deep processing of radix pseudostellariae.
Description
Technical Field
the invention belongs to the field of preparation of radix pseudostellariae active substances, and particularly relates to a preparation method of a radix pseudostellariae extract.
Background
Pseudostellaria root, originally recorded in Bencao Shixin, is the root tuber of Pseudostellaria heterophylla of Caryophyllaceae, and is produced in Fujian, Jiangsu, Anhui, Shandong provinces. 2010 version of Chinese pharmacopoeia describes that radix pseudostellariae is sweet in taste, slightly bitter and mild in nature; spleen and lung meridian entered; has the effects of replenishing qi to invigorate the spleen, promoting the production of body fluid and moistening the lung; can be used for treating spleen deficiency, asthenia, anorexia, spontaneous perspiration, thirst, and lung dryness. The radix pseudostellariae is a variety of 'a list of articles for health care food' issued by the ministry of health, and researches on chemical components of the radix pseudostellariae show that the radix pseudostellariae contains amino acids, saccharides, glycosides, phospholipids, cyclic peptides, fatty acids, oils and fats, volatile components, sterols, trace elements and the like, wherein the radix pseudostellariae polysaccharide is an important component of a biological active substance of the radix pseudostellariae. Modern pharmacological studies have demonstrated that: the radix Pseudostellariae polysaccharide has effects of enhancing immunity, improving learning and memory ability, relieving fatigue, delaying aging, and resisting inflammation; meanwhile, the total glycosides of radix pseudostellariae have strong myocardial anoxia resistance, and the radix pseudostellariae extract has an obvious stimulation effect on lymphocyte.
at present, the utilization of the radix pseudostellariae by people is still in the initial exploration stage, and the pharmacological research on the radix pseudostellariae is continuously and deeply carried out. Meanwhile, the extraction process of the radix pseudostellariae is continuously updated and improved by people, the extraction of the radix pseudostellariae is concentrated on dry radix pseudostellariae as an extraction raw material at present, the extraction process mainly comprises water extraction, crushing extraction, ultrasonic auxiliary extraction and the like, but the extraction efficiency of the radix pseudostellariae extract is still low, so that the waste of the radix pseudostellariae is caused, and the environment pollution is caused due to improper waste treatment. The reason is analyzed, mainly because the radix pseudostellariae is similar to other Chinese herbal medicines, natural and compact lignocelluloses cell tissues playing a skeleton role are formed in the growth of the radix pseudostellariae plant, and the natural and compact lignocelluloses cell tissues form an extraction resistance barrier of effective components and a degradation resistance barrier of subsequent substance transformation, so that the radix pseudostellariae needs to be pretreated before extraction and transformation. In addition, the extraction of the radix pseudostellariae is concentrated on the dried radix pseudostellariae as an extraction raw material at present, the extraction of effective substances of the fresh radix pseudostellariae is not reported, and particularly for radix pseudostellariae utilization enterprises in the origin of the radix pseudostellariae, how to utilize the fresh radix pseudostellariae as the raw material for extracting the effective substances can not only save the process of drying and processing the radix pseudostellariae, obviously reduce the cost and save a large amount of time, but also furthest reduce the pollution of harmful microorganisms in the drying and storage processes.
Disclosure of Invention
in order to overcome the defects in the prior art, the invention aims to provide a preparation method of a radix pseudostellariae extract.
in order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a preparation method of radix pseudostellariae extract comprises the following steps:
S1, preprocessing the fresh radix pseudostellariae: the preparation method comprises the following steps of sequentially carrying out four procedures of cleaning, removing fibrous roots, airing and cutting off the fresh radix pseudostellariae; wherein, the cleaning is to dig out the fresh pseudostellaria heterophylla plants in the current year from the soil and clean the fresh pseudostellaria heterophylla plants with clear water to wash away soil and other impurities on the surfaces of the pseudostellaria heterophylla plants; the removal of the fibrous root means that the cleaned tail fibrous root of the radix pseudostellariae is manually cut off; the airing refers to airing the radix pseudostellariae after the beard is removed for 6-10 hours; cutting off means cutting off the air-dried radix Pseudostellariae from the center of radix Pseudostellariae body to divide it into two parts;
s2, double-stage steam explosion treatment: the cut radix pseudostellariae is taken as a blasting matrix, and steam blasting treatment I and steam blasting treatment II are carried out in sequence, wherein the interval time of the two-stage steam blasting treatment is 2-5 min; the conditions of the steam explosion treatment I are as follows: the charging coefficient of the blasting cavity is 0.7-0.9, the pressure of blasting steam is 0.5-0.8 MPa, the pressure maintaining treatment time of blasting is 3-10 min, and the blasting time is not higher than 0.00875 s; the conditions of the steam explosion treatment II are as follows: performing same-cavity blasting treatment on the same blasting equipment, wherein the pressure of blasting steam reaches 1.5-2.2 MPa, the pressure maintaining treatment time of blasting is 10-50 s, and the blasting time is not higher than 0.00875 s; adding an entrainer in the double-section steam explosion treatment process, wherein the entrainer is a compound of fine-mesh carborundum and Ca (OH)2 powder, and the carborundum: the mass ratio of the Ca (OH)2 powder is 4: 1-1: 1; the total addition amount of the entrainer accounts for 1.5-3% of the blasting matrix by mass percent, the entrainer is added in batches, 1/3-2/5 of the total amount of the entrainer is added in the steam blasting treatment I, and the entrainer is added in the rest amount in the steam blasting treatment II;
s3, hot water extraction: collecting the exploded radix pseudostellariae matrix, feeding the collected radix pseudostellariae matrix into a hot water extraction tank, wherein the mass ratio of the hot water amount in the tank to the radix pseudostellariae matrix is 15: 1-20: 1, the temperature of the extracted hot water is 90-100 ℃, the extraction time is 2-3 hours, and the extraction times are 1 time; stirring at a constant speed (the rotating speed is 90-120 rpm/min) in the early stage of extraction, and stopping stirring when the extraction is carried out for 3/5 extraction time;
S4, extract CO2 treatment: after hot water extraction is finished, firstly cooling the obtained extraction liquid to room temperature by using a heat exchanger, then transferring the extraction liquid into a clean stainless steel container, then introducing sterile CO2 gas into the cooled extraction liquid, wherein the flow rate of CO2 is 10-15 m3/min, and the time of introducing CO2 gas is 5-15 min; when CO2 gas is introduced, the CO2 gas enters the inside of the extraction liquid through a pipeline and a gas distributor at the bottom of the stainless steel container;
s5, three-stage filtration: after the extraction liquid is treated by CO2, the extraction liquid is filtered by a first-stage filter, a second-stage filter and a third-stage filter which are combined in series respectively; wherein the primary filter and the secondary filter are both conventional cylindrical filters, and the filter element pore diameters of the primary filter and the secondary filter are respectively 4.5-5 μm and 0.2-0.25 μm; the third-stage filter is a hollow fiber filter, and the molecular weight cut-off of the hollow fiber is 10000-30000 daltons;
s6, detoxification: and (3) enabling the radix pseudostellariae extract subjected to the three-stage filtration to pass through a D301 resin packed column and a NKAII resin packed column in sequence, enabling the column pressure of the extract to be 0.15-0.2 MPa, and finally collecting the extract to obtain the radix pseudostellariae extract.
Preferably, a self-made three-stage filtration system is used for carrying out three-stage filtration in S5, and the three-stage filtration system comprises a tubular centrifuge, a centrifugate storage tank (the temperature control range in the tank is 5-10 ℃), a power transmission component I, a power transmission component II, a power transmission component III, a first-stage filter, a second-stage filter, a third-stage filter and an external auxiliary system thereof; the power transmission assembly I is formed by connecting a power pump I and a check valve I in series; the power transmission assembly II is formed by connecting a power pump II and a check valve II in series; the power transmission assembly III is formed by connecting a power pump III and a check valve III in series; the first-stage filter and the second-stage filter are conventional cylinder filters; the third-stage filter is a self-made filter and structurally comprises a cylinder, a hollow fiber bundle assembly and a base; the bottom and the top of the cylinder are respectively provided with a liquid inlet and a liquid outlet, the outside of the cylinder is provided with an interlayer, and the upper end and the lower end of the interlayer are respectively provided with an interlayer inlet and an interlayer outlet; the hollow fiber bundle component comprises a component shell with uniformly distributed multiple holes and a plurality of hollow fibers (preferably 300-600), and the hollow fibers are combined into a bundle and placed in the component shell; the base is a cylinder, the upper section of the cylinder is hollow and cylindrical, the middle lower section of the cylinder is solid, and through holes matched with the number and the size of the hollow fibers are formed in the middle lower section of the solid of the base; the base is positioned below the liquid outlet, clings to the top wall of the cylinder and is fixedly connected with the top wall of the cylinder; the upper end of the hollow fiber is open and penetrates through the through hole of the base, and the lower end of the hollow fiber is closed and is fixedly connected with the bottom wall of the component shell; the external auxiliary system of the three-stage filter comprises a power pump IV, a check valve IV and a constant-temperature refrigeration water tank (the controllable temperature range is 5-15 ℃), wherein an outlet of an interlayer is sequentially connected with the check valve IV, the constant-temperature refrigeration water tank and the power pump IV in series to the inlet of the interlayer to form a closed circulating system; the tube centrifuge is sequentially connected in series with a centrifugate storage tank, a power pump I, a check valve I, a primary filter, a power pump II, a check valve II, a secondary filter, a power pump III, a check valve III and a liquid inlet of a tertiary filter through pipelines; the filter element aperture of the first-stage filter and the second-stage filter is respectively 4.5-5 μm and 0.2-0.22 μm; the molecular weight cut-off of the hollow fiber is 10000-30000 daltons;
The third-stage filtration step is as follows:
(a) firstly, inputting the extraction liquid treated by S4 into an inner cavity of a tubular centrifuge, starting the equipment to carry out centrifugation, wherein the centrifugation speed reaches 4000-6000 rpm/min, and the centrifugation lasts 20-30 min; after the centrifugation is finished, timely transferring the obtained centrifugate into a centrifugate storage tank, wherein the temperature control range in the tank is 5-10 ℃;
(b) starting a power pump I to convey the cooling centrifugate obtained in the previous procedure into a primary filter through a check valve I by a pipeline, and realizing primary coarse filtration by inputting pressure of 0.2-0.6 Mpa;
(c) Starting a power pump II, conveying the primary filtrate obtained after the primary filter to a secondary filter through a check valve II by a pipeline, and inputting the pressure of 0.2-0.6 Mpa to realize secondary coarse filtration;
(d) Starting a power pump IV, enabling cold water with the temperature of 5-15 ℃ prepared in the constant-temperature refrigeration water tank to enter the interlayer from the interlayer inlet through a pipeline, and enabling the cold water to pass through a check valve IV from the interlayer outlet and to be recycled to the constant-temperature refrigeration water tank; and when the temperature of the inner cavity of the cylinder body reaches 5-15 ℃, starting the power pump III, conveying a secondary filtrate obtained after passing through the secondary filter into the tertiary filter from the liquid inlet through the check valve III by a pipeline, wherein the input pressure is 0.2-0.6 MPa, the secondary filtrate firstly enters the inside of the component shell from the porous part on the component shell, impurities in the secondary filtrate are blocked outside the hollow fibers due to the interception effect of the hollow fibers, and the extract liquid passes through the hollow fibers and enters the base from the top opening of the hollow fibers and then comes out from the liquid outlet.
further, S5 further includes step (e) back washing: when the filtration work is finished, the reverse cleaning operation of the system can be carried out, the directions of the power transmission assembly I, the power transmission assembly II and the power transmission assembly III are integrally adjusted by 180 degrees manually, namely the original inlets are changed into outlets, then corresponding pipelines are connected, the power pump I, the power pump II and the power pump III are started simultaneously, clean purified water enters the cylinder through the liquid outlet of the cylinder, then the cleaning water is finally discharged from the centrifugal liquid storage tank, the pointers of pressure gauges of the filters in the operation are not higher than 0.3 MPa, and the operation is repeated for 2-3 times.
preferably, pipelines between the centrifugate storage tank and the power pump I, between the check valve I and the primary filter, between the primary filter and the power pump II, between the check valve II and the secondary filter, between the secondary filter and the power pump III and between the check valve III and the tertiary filter are movably connected.
furthermore, the movable connection mode is a clamp connection mode.
Preferably, the tubular centrifuge is of the GF-separation type.
the steam explosion technology can release the compressed gas infiltrated into the plant tissue in a short time and suddenly, and aims to decompose the raw material according to requirements by using less energy. The steam explosion suspends all the raw materials in the container in the air space in a burst mode within a millisecond time range. Because the action time is short, the energy density is high and concentrated, gas molecules can permeate between macromolecules such as cellulose and lignin, and the materials are fully decomposed on the macromolecule level. Thus, the steam explosion process can break up the co-polymers of lignin, hemicellulose and cellulose embedded with plant cell wall pectin into dispersions of lignin, pectin, hemicellulose and cellulose. The invention just considers that the steam explosion technology can destroy the natural barrier system of the plant cell wall, thereby adopting the steam explosion technology as the pretreatment procedure before the extraction of the radix pseudostellariae, and being beneficial to the extraction and the separation of the effective components of the radix pseudostellariae. However, the pretreatment of the radix pseudostellariae by the steam explosion technology still has partial problems, for example, certain toxic substances such as formic acid, furfural, phenolic compounds and the like can be generated by lignocellulose of plant cell walls in the high-temperature and high-pressure process of steam explosion. Therefore, the entrainer is added in the double-section steam explosion stage of the invention, the carborundum has stronger shearing action at the moment of steam explosion, the cell natural barrier system of the radix pseudostellariae is further broken, and the addition of Ca (OH)2 is not beneficial to the generation of toxic substances in the steam explosion process environment, so that the conversion of the toxic substances is reduced. CO2 is introduced into the extract liquid for treatment, CO2 reacts with Ca (OH)2 to generate CaCO3 sediment, which is beneficial to removing carborundum in the three-stage filtration process, and the pH value of the extract liquid is adjusted at the same time, so that the D301 resin packed column and the NKAII resin packed column are acidic, and the detoxification efficiency is improved. In addition, researches show that the plant source Chinese herbal medicine needs to be subjected to enzyme deactivation in the drying process, and the enzyme deactivation is mainly used for removing decomposition enzymes in the Chinese herbal medicine body to reduce the loss of partial effective components. The high-temperature and high-pressure action of the double-section steam explosion can also achieve the aim of enzyme deactivation, and a special enzyme deactivation procedure is omitted. Therefore, the six steps of the preparation method of the invention, namely the pretreatment of the fresh radix pseudostellariae, the two-stage steam explosion treatment, the hot water extraction, the extraction liquid CO2 treatment, the three-stage filtration and the detoxification, supplement each other and show positive synergistic effect.
has the advantages that:
1. The two-stage steam explosion technology not only achieves the effect of extracting and pretreating the radix pseudostellariae, but also achieves the purpose of inactivating enzyme of fresh radix pseudostellariae, and meanwhile, the technology can reduce and even remove toxic substances generated in steam explosion;
2. CO2 is introduced into the extract liquid for treatment, CO2 reacts with Ca (OH)2 to generate CaCO3 sediment, so that not only can the introduced calcium ions be effectively removed, but also the calcium ions and carborundum can be removed together in the three-stage filtration process, and meanwhile, the pH value of the extract liquid is adjusted, so that the D301 resin packed column and the NKAII resin packed column are acidic, and the detoxification efficiency is improved;
3. The method has the characteristics of precise process, easy scale flow standardized operation and use, continuous operation, no need of raw material soaking pretreatment, obvious improvement of the extraction efficiency of the radix pseudostellariae and reduction of the labor intensity;
4. Because the fresh radix pseudostellariae without fibrous roots is adopted as the raw material for extraction, the process of drying and processing the radix pseudostellariae can be omitted, the cost is obviously reduced, a large amount of time is saved, and the pollution of harmful microorganisms in the drying and storage process is reduced to the greatest extent; the preparation method is very suitable for the preparation and production of the radix pseudostellariae extract of the enterprises in the origin of radix pseudostellariae, and provides a new way for the deep processing of radix pseudostellariae.
Drawings
FIG. 1: photo of radix pseudostellariae matrix after blasting treatment;
FIG. 2: the invention discloses a structural schematic diagram of a self-made three-stage filtration system;
FIG. 3: the structure schematic diagram of the self-made three-stage filtration system in the back-washing working state is shown in the invention;
Description of reference numerals: 1-tubular centrifuge, 2-centrifugate storage tank, 31-power pump I, 41-check valve I, 32-power pump II, 42-check valve II, 33-power pump III, 43-check valve III, 34-power pump IV, 44-check valve IV, 5-primary filter, 6-secondary filter, 7-cylinder, 8-liquid inlet, 9-liquid outlet, 10-interlayer, 11-interlayer inlet, 12-interlayer outlet, 13-base, 14-component shell, 15-hollow fiber and 16-constant temperature refrigeration water tank.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and detailed description.
example 1
a preparation method of radix pseudostellariae extract comprises the following steps:
S1, preprocessing the fresh radix pseudostellariae: the preparation method comprises the following steps of sequentially carrying out four procedures of cleaning, removing fibrous roots, airing and cutting off the fresh radix pseudostellariae; wherein, the cleaning is to dig out the fresh pseudostellaria heterophylla plants in the current year from the soil and clean the fresh pseudostellaria heterophylla plants with clear water to wash away soil and other impurities on the surfaces of the pseudostellaria heterophylla plants; the removal of the fibrous root means that the cleaned tail fibrous root of the radix pseudostellariae is manually cut off; the airing refers to airing the radix pseudostellariae after the beard is removed for 10 hours; cutting off means cutting off the air-dried radix Pseudostellariae from the center of radix Pseudostellariae body to divide it into two parts;
s2, double-stage steam explosion treatment: the cut radix pseudostellariae is taken as an explosion matrix, and the steam explosion treatment I and the steam explosion treatment II are carried out in sequence, wherein the interval time of the two-section steam explosion treatment is 3 min; the conditions of the steam explosion treatment I are as follows: the charging coefficient of the blasting cavity is 0.8, the pressure of blasting steam reaches 0.6MPa, the pressure maintaining treatment time of blasting is 6min, and the blasting time is not higher than 0.00875 s; the conditions of the steam explosion treatment II are as follows: performing same-cavity blasting treatment on the same blasting equipment, wherein the pressure of blasting steam reaches 2.0MPa, the pressure maintaining treatment time of blasting is 30s, and the blasting time is not higher than 0.00875 s; adding an entrainer in the double-section steam explosion treatment process, wherein the entrainer is a compound of fine-mesh carborundum and Ca (OH)2 powder, and the carborundum: the mass ratio of Ca (OH)2 powder is 1: 1; the total addition amount of the entrainer accounts for 2% of the blasting matrix by mass percent, the entrainer is added in batches, 1/3 of the total amount is added in the steam blasting treatment I, and the entrainer of the rest amount is added in the steam blasting treatment II; the photograph of the radix pseudostellariae matrix after blasting treatment is shown in figure 1;
s3, hot water extraction: collecting the exploded radix Pseudostellariae matrix, feeding into a hot water extraction tank, wherein the mass ratio of hot water in the tank to radix Pseudostellariae matrix is 18:1, the temperature of extraction hot water is 95 deg.C, the extraction time is 2.5h, and the extraction times are 1 time; stirring at constant speed (the rotating speed is 100 rpm/min) in the early stage of extraction, and stopping stirring when the extraction is carried out for 3/5 extraction time;
S4, extract CO2 treatment: after hot water extraction is finished, firstly cooling the obtained extraction liquid to room temperature by using a heat exchanger, then transferring the extraction liquid into a clean stainless steel container, then introducing sterile CO2 gas into the cooled extraction liquid, wherein the flow rate of CO2 is 13m3/min, and the time of introducing CO2 gas is 10 min; when CO2 gas is introduced, the CO2 gas enters the inside of the extraction liquid through a pipeline and a gas distributor at the bottom of the stainless steel container;
S5, three-stage filtration: performing three-stage filtration by using a self-made three-stage filtration system, as shown in fig. 2, the three-stage filtration system comprises a GF-separation type tubular centrifuge 1, a centrifugate storage tank 2 (the temperature control range in the tank is 5-10 ℃), a power transmission component I, a power transmission component II, a power transmission component III, a first-stage filter 5, a second-stage filter 6, a third-stage filter and an external auxiliary system thereof;
the power transmission assembly I is formed by connecting a power pump I31 and a check valve I41 in series; the power transmission component II is formed by connecting a power pump II 32 and a check valve II 42 in series; the power transmission assembly III is formed by connecting a power pump III 33 and a check valve III 43 in series;
the primary filter 5 and the secondary filter 6 are conventional cartridge filters; the third-stage filter is a self-made filter and structurally comprises a cylinder body 7, a hollow fiber bundle assembly and a base 13; a liquid inlet 8 and a liquid outlet 9 are respectively arranged at the bottom and the top of the cylinder 7, an interlayer 10 is arranged outside the cylinder 7, and an interlayer inlet 11 and an interlayer outlet 12 are respectively arranged at the upper end and the lower end of the interlayer 10; the hollow fiber bundle component comprises a component shell 14 with a plurality of holes uniformly distributed, 600 hollow fibers 15 with the molecular weight cutoff of 10000 Dalton, and the hollow fibers 15 are combined into a bundle and arranged in the component shell 14; the base 13 is a cylinder, the upper section of the cylinder is hollow and cylindrical, the middle lower section of the cylinder is solid, and through holes matched with the quantity and the size of the hollow fibers 15 are formed in the middle lower section of the solid of the base 13; the base 13 is positioned below the liquid outlet 9, clings to the top wall of the cylinder 7 and is fixedly connected with the cylinder; the upper end of the hollow fiber 15 is open and penetrates through the through hole of the base 13, and the lower end is closed and fixedly connected with the bottom wall of the component shell 14;
The external auxiliary system of the three-stage filter comprises a power pump IV 34, a check valve IV 44 and a constant-temperature refrigeration water tank 16, wherein an interlayer outlet 12 is sequentially connected with the check valve IV 44, the constant-temperature refrigeration water tank 16 (the controllable temperature range is 5-15 ℃) and the power pump IV 34 to an interlayer inlet 11 in series to form a closed circulating system;
The tube centrifuge 1 is sequentially connected in series with a centrifugate storage tank 2, a power pump I31, a check valve I41, a primary filter 5, a power pump II 32, a check valve II 42, a secondary filter 6, a power pump III 33, a check valve III 43 to a liquid inlet 8 of a tertiary filter through pipelines; wherein, the pipelines between the centrifugate storage tank 2 and the power pump I31, between the check valve I41 and the primary filter 5, between the primary filter 5 and the power pump II 32, between the check valve II 42 and the secondary filter 6, between the secondary filter 6 and the power pump III 33, and between the check valve III 43 and the tertiary filter are connected by clips;
the primary filter 5 and the secondary filter 6 are commercially available, the filter elements of the primary filter 5 and the secondary filter 6 are all folding filter elements, the number of the filter elements of the primary filter 5 and the secondary filter 6 is respectively 5 and 7, and the pore diameters of the filter elements of the primary filter 5 and the secondary filter 6 are respectively 5 micrometers and 0.22 micrometer; the filter element materials of the primary filter 5 and the secondary filter 6 are polypropylene, the filter element framework (support frame) is hard plastic, and the filter element can resist the pressure of 0.4 MPa;
the third-stage filtration step is as follows:
(a) Firstly, inputting the extract obtained in S4 into the inner cavity of a tubular centrifuge 1, starting the equipment to carry out centrifugation, wherein the centrifugation speed reaches 5000rpm/min, and the centrifugation is carried out for 30 min; after the centrifugation is finished, timely transferring the obtained centrifugate into a centrifugate storage tank 2, wherein the temperature control range in the tank is 10 ℃;
(b) Starting a power pump I31 to convey the cooling centrifugate obtained in the previous procedure into a primary filter 5 through a check valve I41 by a pipeline, and realizing primary coarse filtration by input pressure of 0.2 Mpa;
(c) starting the power pump II 32 to convey the primary filtrate obtained after passing through the primary filter 5 into the secondary filter 6 through a pipeline by a check valve II 42, and inputting the pressure of 0.2Mpa to realize secondary coarse filtration;
(d) starting a power pump IV 34, and enabling cold water with the temperature of 5 ℃ prepared in the constant-temperature refrigeration water tank 16 to enter the interlayer 10 from the interlayer inlet 11 through a pipeline, and enabling the cold water to pass through a check valve IV 44 from the interlayer outlet 12 and then to be recycled into the constant-temperature refrigeration water tank 16; when the temperature of the inner cavity of the cylinder 7 reaches 5 ℃, the power pump III 33 is started to convey the secondary filtrate obtained after passing through the secondary filter 6 into the tertiary filter from the liquid inlet 8 through the check valve III 43 by a pipeline, the input pressure is 0.2Mpa, the secondary filtrate firstly enters the inside of the component shell 14 from the porous hole on the component shell 14, due to the interception function of the hollow fiber 15, the impurities in the secondary filtrate are blocked outside the hollow fiber 15, and the extract liquid passes through the hollow fiber 15 and enters the base 13 from the top opening of the hollow fiber 15 and then comes out from the liquid outlet 9;
(e) reverse cleaning of the device: when the filtration work is finished, the reverse cleaning operation of the series of devices can be carried out, namely, the clamps on the pipelines between the centrifugate storage tank 2 and the power pump I31, between the check valve I41 and the primary filter 5, between the primary filter 5 and the power pump II 32, between the check valve II 42 and the secondary filter 6, between the secondary filter 6 and the power pump III 33 and between the check valve III 43 and the tertiary filter are firstly disassembled, the directions of the power transmission component I, the power transmission component II and the power transmission component III are manually adjusted by 180 degrees (the original inlet is changed into the outlet), then the power transmission component II and the power transmission component III are connected with the corresponding pipelines through the clamps, the adjusted state is shown in figure 3, the power pump I31, the power pump II 32 and the power pump III 33 are simultaneously started, clean purified water enters the cylinder 7 through the liquid outlet 9 of the cylinder 7, and finally is discharged from the centrifugate storage tank 2, the pointer of each filter pressure gauge in the operation is not higher than 0.3 MPa, and the operation is repeated for 2 times;
s6, detoxification: and (3) sequentially passing the radix pseudostellariae extract subjected to the three-stage filtration through a D301 resin packed column and a NKAII resin packed column, wherein the column pressure of the extract is 0.15MPa, and finally collecting the extract to obtain the radix pseudostellariae extract.
comparative example 1
The difference from example 1 is that S2: entrainers are not added in the steam explosion treatment I and the steam explosion treatment II; the other steps are the same as in example 1.
comparative example 2
the difference from example 1 is that S2: only carrying out steam explosion treatment I, and adding the full amount of entrainer; the other steps are the same as in example 1.
comparative example 3
The difference from example 1 is that S2: only carrying out steam explosion treatment II and adding the full amount of entrainer; the other steps are the same as in example 1.
The content of the radix pseudostellariae extract obtained in example 1 and comparative examples 1 to 3, and the content of polysaccharide, saponin and cyclic peptide therein were respectively detected, and the respective extraction rates were calculated, wherein the extraction rate = (mass of corresponding detection item/fresh radix pseudostellariae matrix) × 100%, and the fresh radix pseudostellariae matrix, that is, the fresh radix pseudostellariae treated in S1, and the results are as follows:
As can be seen from the above table:
(1) compared with the example 1, the extraction rate of the radix pseudostellariae extract in the comparative example 1 is obviously lower than that in the example 1, which shows that the extraction rate of the radix pseudostellariae can be obviously improved by adding the entrainer compared with not adding the entrainer;
(2) compared with example 1, the extraction rate of the radix pseudostellariae extract in the comparative examples 2 and 3 is obviously lower than that in the example 1, which shows that the extraction rate of the radix pseudostellariae extract can be obviously improved by the two-stage steam explosion compared with the one-stage steam explosion.
In conclusion, the method adopts double-stage steam explosion, and the entrainer is added in the process, so that the extraction rate of the radix pseudostellariae extract is improved to the greatest extent.
Claims (6)
1. A preparation method of a radix pseudostellariae extract is characterized by comprising the following steps:
S1, preprocessing the fresh radix pseudostellariae: the preparation method comprises the following steps of sequentially carrying out four procedures of cleaning, removing fibrous roots, airing and cutting off the fresh radix pseudostellariae;
S2, double-stage steam explosion treatment: the cut radix pseudostellariae is taken as a blasting matrix, and steam blasting treatment I and steam blasting treatment II are carried out in sequence, wherein the interval time of the two-stage steam blasting treatment is 2-5 min; the conditions of the steam explosion treatment I are as follows: the charging coefficient of the blasting cavity is 0.7-0.9, the pressure of blasting steam is 0.5-0.8 MPa, the pressure maintaining treatment time of blasting is 3-10 min, and the blasting time is not higher than 0.00875 s; the conditions of the steam explosion treatment II are as follows: performing same-cavity blasting treatment on the same blasting equipment, wherein the pressure of blasting steam reaches 1.5-2.2 MPa, the pressure maintaining treatment time of blasting is 10-50 s, and the blasting time is not higher than 0.00875 s; adding an entrainer in the double-section steam explosion treatment process, wherein the entrainer is a compound of fine-mesh carborundum and Ca (OH)2 powder, and the carborundum: the mass ratio of the Ca (OH)2 powder is 4: 1-1: 1; the total addition amount of the entrainer accounts for 1.5-3% of the blasting matrix by mass percent, the entrainer is added in batches, 1/3-2/5 of the total amount of the entrainer is added in the steam blasting treatment I, and the entrainer is added in the rest amount in the steam blasting treatment II;
s3, hot water extraction: collecting the exploded radix pseudostellariae matrix, feeding the collected radix pseudostellariae matrix into a hot water extraction tank, wherein the mass ratio of the hot water amount in the tank to the radix pseudostellariae matrix is 15: 1-20: 1, the temperature of the extracted hot water is 90-100 ℃, the extraction time is 2-3 hours, and the extraction times are 1 time; stirring at constant speed in the early stage of extraction, and stopping stirring when the extraction is carried out for 3/5 extraction time;
S4, extract CO2 treatment: after hot water extraction is finished, cooling the obtained extraction liquid to room temperature, transferring the extraction liquid into a clean stainless steel container, introducing sterile CO2 gas into the cooled extraction liquid, wherein the flow rate of CO2 is 10-15 m3/min, and introducing CO2 gas for 5-15 min;
s5, three-stage filtration: after the extraction liquid is treated by CO2, the extraction liquid is filtered by a first-stage filter, a second-stage filter and a third-stage filter which are combined in series respectively; wherein the primary filter and the secondary filter are both conventional cylindrical filters, and the filter element pore diameters of the primary filter and the secondary filter are respectively 4.5-5 μm and 0.2-0.25 μm; the third-stage filter is a hollow fiber filter, and the molecular weight cut-off of the hollow fiber is 10000-30000 daltons;
S6, detoxification: and (3) enabling the radix pseudostellariae extract subjected to the three-stage filtration to pass through a D301 resin packed column and a NKAII resin packed column in sequence, enabling the column pressure of the extract to be 0.15-0.2 MPa, and finally collecting the extract to obtain the radix pseudostellariae extract.
2. the method of claim 1, wherein: s5, performing three-stage filtration by using a self-made three-stage filtration system, wherein the three-stage filtration system comprises a tubular centrifuge, a centrifugate storage tank, a power transmission component I, a power transmission component II, a power transmission component III, a first-stage filter, a second-stage filter, a third-stage filter and an external auxiliary system thereof; the power transmission assembly I is formed by connecting a power pump I and a check valve I in series; the power transmission assembly II is formed by connecting a power pump II and a check valve II in series; the power transmission assembly III is formed by connecting a power pump III and a check valve III in series; the first-stage filter and the second-stage filter are conventional cylinder filters; the third-stage filter is a self-made filter and structurally comprises a cylinder, a hollow fiber bundle assembly and a base; the bottom and the top of the cylinder are respectively provided with a liquid inlet and a liquid outlet, the outside of the cylinder is provided with an interlayer, and the upper end and the lower end of the interlayer are respectively provided with an interlayer outlet and an interlayer inlet; the hollow fiber bundle component comprises a component shell with a plurality of holes uniformly distributed and a plurality of hollow fibers, and the hollow fibers are combined into a bundle and arranged in the component shell; the base is a cylinder, the upper section of the cylinder is hollow and cylindrical, the middle lower section of the cylinder is solid, and through holes matched with the number and the size of the hollow fibers are formed in the middle lower section of the solid of the base; the base is positioned below the liquid outlet, clings to the top wall of the cylinder and is fixedly connected with the top wall of the cylinder; the upper end of the hollow fiber is open and penetrates through the through hole of the base, and the lower end of the hollow fiber is closed and is fixedly connected with the bottom wall of the component shell; the external auxiliary system of the three-stage filter comprises a power pump IV, a check valve IV and a constant-temperature refrigeration water tank, wherein an interlayer outlet is sequentially connected with the check valve IV, the constant-temperature refrigeration water tank and the power pump IV in series to an interlayer inlet to form a closed circulating system; the tube centrifuge is sequentially connected in series with a centrifugate storage tank, a power pump I, a check valve I, a primary filter, a power pump II, a check valve II, a secondary filter, a power pump III, a check valve III and a liquid inlet of a tertiary filter through pipelines; the filter element aperture of the first-stage filter and the second-stage filter is respectively 4.5-5 μm and 0.2-0.22 μm; the molecular weight cut-off of the hollow fiber is 10000-30000 daltons;
The third-stage filtration step is as follows:
(a) firstly, inputting the extraction liquid treated by S4 into an inner cavity of a tubular centrifuge, starting the equipment to carry out centrifugation, wherein the centrifugation speed reaches 4000-6000 rpm/min, and the centrifugation lasts 20-30 min; after the centrifugation is finished, timely transferring the obtained centrifugate into a centrifugate storage tank, wherein the temperature control range in the tank is 5-10 ℃;
(b) starting a power pump I to convey the cooling centrifugate obtained in the previous procedure into a primary filter through a check valve I by a pipeline, and realizing primary coarse filtration by inputting pressure of 0.2-0.6 Mpa;
(c) starting a power pump II, conveying the primary filtrate obtained after the primary filter to a secondary filter through a check valve II by a pipeline, and inputting the pressure of 0.2-0.6 Mpa to realize secondary coarse filtration;
(d) Starting a power pump IV, enabling cold water with the temperature of 5-15 ℃ prepared in the constant-temperature refrigeration water tank to enter the interlayer from the interlayer inlet through a pipeline, and enabling the cold water to pass through a check valve IV from the interlayer outlet and to be recycled to the constant-temperature refrigeration water tank; and when the temperature of the inner cavity of the cylinder body reaches 5-15 ℃, starting the power pump III, conveying a secondary filtrate obtained after passing through the secondary filter into the tertiary filter from the liquid inlet through the check valve III by a pipeline, wherein the input pressure is 0.2-0.6 MPa, the secondary filtrate firstly enters the inside of the component shell from the porous part on the component shell, impurities in the secondary filtrate are blocked outside the hollow fibers due to the interception effect of the hollow fibers, and the extract liquid passes through the hollow fibers and enters the base from the top opening of the hollow fibers and then comes out from the liquid outlet.
3. The method of claim 2, wherein S5 further comprises step (e) back washing: when the filtration work is finished, the reverse cleaning operation of the system can be carried out, the directions of the power transmission assembly I, the power transmission assembly II and the power transmission assembly III are integrally adjusted by 180 degrees manually, namely the original inlets are changed into outlets, then corresponding pipelines are connected, the power pump I, the power pump II and the power pump III are started simultaneously, clean purified water enters the cylinder through the liquid outlet of the cylinder, then the cleaning water is finally discharged from the centrifugal liquid storage tank, the pointers of pressure gauges of the filters in the operation are not higher than 0.3 MPa, and the operation is repeated for 2-3 times.
4. the method of claim 2, wherein: the pipelines between the centrifugate storage tank and the power pump I, between the check valve I and the primary filter, between the primary filter and the power pump II, between the check valve II and the secondary filter, between the secondary filter and the power pump III and between the check valve III and the tertiary filter adopt a movable connection mode.
5. the method of claim 4, wherein: the movable connection mode is clamp connection.
6. The method of claim 2, wherein: the tubular centrifuge is of GF-separation type.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610759126.6A CN106176882B (en) | 2016-08-30 | 2016-08-30 | preparation method of radix pseudostellariae extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610759126.6A CN106176882B (en) | 2016-08-30 | 2016-08-30 | preparation method of radix pseudostellariae extract |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106176882A CN106176882A (en) | 2016-12-07 |
| CN106176882B true CN106176882B (en) | 2019-12-06 |
Family
ID=58089448
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610759126.6A Active CN106176882B (en) | 2016-08-30 | 2016-08-30 | preparation method of radix pseudostellariae extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106176882B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109170326A (en) * | 2018-09-30 | 2019-01-11 | 福建贝迪药业有限公司 | A kind of compound probiotics and preparation method thereof beneficial to improving performance of poultry |
| CN109287884A (en) * | 2018-09-30 | 2019-02-01 | 福建贝迪药业有限公司 | A kind of compound Chinese herb Tiny ecosystem pulvis and preparation method thereof being exclusively used in egg fowl egg-laying peak |
| CN108935962A (en) * | 2018-09-30 | 2018-12-07 | 福建贝迪药业有限公司 | A kind of pulvis and preparation method thereof taking off mould protect liver for livestock and poultry |
| CN113975374B (en) * | 2021-10-20 | 2023-09-12 | 成都普睿法药物研发有限公司 | Application of radix pseudostellariae cyclic peptide B in preparation of medicines for resisting experimental pulmonary fibrosis |
| CN115403574A (en) * | 2022-08-03 | 2022-11-29 | 李玉山 | Preparation method of rauwolfine hydrochloride |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006325457A (en) * | 2005-05-25 | 2006-12-07 | Daicho Kikaku:Kk | Health food |
| CN102293810A (en) * | 2011-07-05 | 2011-12-28 | 食品行业生产力促进中心 | Pseudostellaria root functional component extraction method by ultrasonic |
| CN103006746A (en) * | 2012-12-31 | 2013-04-03 | 福建中医药大学 | Radix pseudostellariae extracts and application thereof |
| CN103923191A (en) * | 2014-04-28 | 2014-07-16 | 中国科学院昆明植物研究所 | Preparation method of standard radix pseudostellariae cyclopeptide B |
| CN104173412B (en) * | 2014-05-21 | 2017-10-03 | 厦门医学院 | The purposes of radix pseudostellariae extract |
-
2016
- 2016-08-30 CN CN201610759126.6A patent/CN106176882B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN106176882A (en) | 2016-12-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106214725B (en) | Preparation method of radix pseudostellariae fibrous extract | |
| CN106176882B (en) | preparation method of radix pseudostellariae extract | |
| CN100473656C (en) | Method for preparing extractive of olive leaves rich in oleuropein in high purity | |
| CN101095724B (en) | Technics for extracting lotus leaf flavone | |
| CN101701013A (en) | Method for extracting proanthocyanidin from pine bark by taking water as solvent | |
| CN108385421B (en) | lignocellulose supercritical CO2Blasting and component separation method thereof | |
| CN107982305A (en) | A kind of method of steam explosion assisted extraction astragaloside and astragalus polyose | |
| CN100522982C (en) | Production process for extracting tea saponin from tea-oil tree cake by using water as dissoluent | |
| CN102477104A (en) | Method for separating and purifying polysaccharide from Hovenia acerba | |
| CN102268827A (en) | Method for extracting celluloses from tobaccos based on ferric chloride pretreatment | |
| CN102558377A (en) | Preparation method of soybean polysaccharide gum | |
| CN114515008A (en) | Cistanche tubulosa extract and preparation method thereof | |
| CN105330670B (en) | A kind of method extracting parthenolide from leaf of Magnolia gradiflora continuous high-efficient | |
| CN101792394B (en) | Extraction separation method of L-synephrine | |
| CN1266160C (en) | Process for extracting rubusoside of fruit of Gorsvenor Momordica | |
| CN110054706A (en) | The method for the fucoidin concentration extracted in one main laminaria | |
| CN105294873A (en) | Extraction method for laminarin | |
| CN101643430B (en) | Method for extracting seaweed organic iodine from seaweed or seaweed root | |
| CN106366206B (en) | A kind of method that steam explosion combination biological enzyme technology prepares radix pseudostellariae source polysaccharide | |
| CN102807565A (en) | Improved method for extracting berberine | |
| CN112106989A (en) | Sweet potato stem leaf polyphenol and extraction method thereof | |
| CN105481872B (en) | A kind of method of constant temperature gradient series connection extraction active components of plants | |
| CN110339225A (en) | A kind of extracting method of Siraitia grosvenorii | |
| CN213431819U (en) | Preparation system for extracting polysaccharide flavone alkaloid from Moraceae plant liquid | |
| CN115521387B (en) | Acidic polysaccharide and method for separating acidic polysaccharide from basil seed gum |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20161207 Assignee: FUJIAN NINGDE BEIDI BIOTECHNOLOGY CO.,LTD. Assignor: FUJIAN BRADY PHARMACEUTICAL CO.,LTD. Contract record no.: X2023980051562 Denomination of invention: A preparation method for the extract of Panax notoginseng Granted publication date: 20191206 License type: Common License Record date: 20231211 |
|
| EE01 | Entry into force of recordation of patent licensing contract |