CN102293810A - Pseudostellaria root functional component extraction method by ultrasonic - Google Patents
Pseudostellaria root functional component extraction method by ultrasonic Download PDFInfo
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- CN102293810A CN102293810A CN 201110185905 CN201110185905A CN102293810A CN 102293810 A CN102293810 A CN 102293810A CN 201110185905 CN201110185905 CN 201110185905 CN 201110185905 A CN201110185905 A CN 201110185905A CN 102293810 A CN102293810 A CN 102293810A
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Abstract
The invention relates to an ultrasonic method for extracting functional components in a medicinal material of pseudostellaria root. The method compises the following steps: (1) crushing or dicing the pseudostellaria root rhizome and adding a proper amount of an alcoholic solution; (2) carrying out an ultrasonic reinforced extraction on the treated pseudostellaria root from step (1) in a water-bath; (3) filtering the extract obtained from step (2), recovering a solvent with a reduced pressure, concentrating to a stiff paste, and obtaining the pseudostellaria root extractive. The method of the invention is simple, convenient and fast for extraction, and with a short extraction time. A recovery rate of functional components reaches more than 70%; a pseudostellaria root polysaccharide content in the extractive reached more than 40%; and a saponins content reaches more 1.5%.
Description
Technical field
The present invention relates to the method for extracts active ingredients and exploitation in a kind of natural product, be specially and use ultrasonic technique from Radix Pseudostellariae, to extract the method for functional components such as polysaccharide and saponin.
Background technology
Radix Pseudostellariae is the dried root of pinkwort Radix Ginseng, has century-old medicinal history in China, has effects such as supplementing QI for promoting the production of body fluid, tonifying the lung spleen invigorating.Its major function composition is polysaccharide and Saponin class material, and material such as aminoacid, sterols, cyclic peptide.Modern study shows that Radix Pseudostellariae extract has resisting fatigue, anti-stress effect, can obviously prolong mice swimming with a load attached to the body time and the time-to-live under the normobaric hypoxia situation; Have immunologic enhancement, can be obviously the rat cell immunologic hypofunction due to the rabbit Chinese People's Anti-Japanese Military and Political College mouse lymphotactin serum (ALS) be had obvious raising effect; Radix Pseudostellariae extract also has effects such as life-saving and antitussive in addition.In the prior art, the extraction of functional component is mainly traditional decocting method and water extraction in the Radix Pseudostellariae, this method exist extraction time long, extraction ratio is low, energy consumption is high, functional component is destroyed problems such as serious.
Ultrasound wave be frequency greater than the mechanical wave more than 20 kHz, have characteristics such as frequency height, wavelength is short, power is big, penetration power is strong.When it is propagated in liquid medium, can produce cavitation and a series of special effects (as mechanical effect, heat effect, chemical effect, biological effect etc.), have some physics, chemistry and the biological nature of quickening to change material: particularly can cause animal, plant, the isocellular collapse of microorganism to be broken, help the solvent infiltration, and the release of strengthening composition in the born of the same parents, help the dissolving and the diffusion of composition in the born of the same parents; And, as long as the suitable ultrasound intensity of control just can not cause the variation of chemical constituent structure and character.Supersonic leaching can shorten leaching time greatly, improves extraction rate, particularly for the low temperature leaching of heat-sensitive ingredients, shows the special technique advantage.
Summary of the invention
The object of the present invention is to provide a kind of method that adopts ultrasound assisted extraction technique to extract the Radix Pseudostellariae functional component, it has overcome traditional decoction method length consuming time, extraction efficiency is low, extraction ratio is low, functional component is lost shortcomings such as serious.
The technical solution used in the present invention is: a kind of method of supersound extraction Radix Pseudostellariae functional component is characterized in that may further comprise the steps:
(1) with after pulverizing of Radix Pseudostellariae rhizome or the pelletizing, immerses alcoholic solution;
(2) with Radix Pseudostellariae solution mixture ultrasound-enhanced extraction under water-bath of step (1);
(3) with the ultrasonic extracting liquid filtering that obtains of step (2), and decompression and solvent recovery, be concentrated into the thick paste shape, obtain Radix Pseudostellariae extract.
In the described step (1), the concentration of alcoholic solution is 15%～95%, and addition is: 4～16 ml/g Radix Angelicae Sinensis.Supersonic frequency is 20kHZ～100kHz in the described step (2), and intensity is 0.1～0.5W/cm
2Bath temperature is 25 ℃～65 ℃ in the described step (2), and extraction time is 10 min～100min.
The present invention compared with prior art has the following advantages:
L, the present invention are raw material with the Radix Pseudostellariae, adopt ultrasonic technique to extract, and have improved extraction ratio, and polyoses content reaches 40% in the extract simultaneously, and saponin content reaches 1.5%;
2, the present invention adopts ultrasonic technique, has shortened extraction time effectively, generally at 10 min～100min
3, technological process is simple, and is easy to control.
The specific embodiment
Embodiment 1:
The Radix Pseudostellariae rhizome is pulverized, and granule size is the 10-20 order, gets the 50g Radix Pseudostellariae, adds 95% ethanol 300ml, and bath temperature is 25 ℃, and the supersound extraction time is 30 minutes, and supersonic frequency is 50kHz, and ultrasound intensity is 0.4W/cm
2, with the ultrasonic extracting liquid filtering that obtains, and decompression and solvent recovery, be concentrated into the thick paste shape, obtain extract, wherein the Radix Pseudostellariae polysaccharide extract rate is 68%, and content is 40%, and Radix Pseudostellariae Saponin extraction ratio is 72%, and content is 1.5%.
Embodiment 2:
With the chopping of Radix Pseudostellariae rhizome, length is the 40-60 order, gets the 50g Radix Pseudostellariae, adds 55% ethanol 800ml, and bath temperature is 65 ℃, and the supersound extraction time is 20 minutes, and supersonic frequency is 50kHz, and ultrasound intensity is 0.3W/cm
2, with the ultrasonic extracting liquid filtering that obtains, and decompression and solvent recovery, be concentrated into the thick paste shape, obtain extract, wherein the Radix Pseudostellariae polysaccharide extract rate is 72%, and content is 39%, and Radix Pseudostellariae Saponin extraction ratio is 74%, and content is 1.6%.
Embodiment 3:
The Radix Pseudostellariae rhizome is pulverized, and granule size is the 20-40 order, gets the 50g Radix Pseudostellariae, adds 75% ethanol 500ml, and bath temperature is 35 ℃, and the supersound extraction time is 40 minutes, and supersonic frequency is 90kHz, and ultrasound intensity is 0.5W/cm
2, with the ultrasonic extracting liquid filtering that obtains, and decompression and solvent recovery, be concentrated into the thick paste shape, obtain extract, wherein the Radix Pseudostellariae polysaccharide extract rate is 72%, and content is 41%, and Radix Pseudostellariae Saponin extraction ratio is 72%, and content is 1.6%.
Embodiment 4:
The Radix Pseudostellariae rhizome is pulverized, and granule size is the 60-80 order, gets the 50g Radix Pseudostellariae, adds 35% ethanol 400ml, and bath temperature is 45 ℃, and the supersound extraction time is 100 minutes, and supersonic frequency is 25kHz, and ultrasound intensity is 0.2 W/cm
2, with the ultrasonic extracting liquid filtering that obtains, and decompression and solvent recovery, be concentrated into the thick paste shape, obtain extract, wherein the Radix Pseudostellariae polysaccharide extract rate is 69%, and content is 40%, and Radix Pseudostellariae Saponin extraction ratio is 65%, and content is 1.2%.
Claims (4)
1. the method for functional component in the supersound extraction Radix Pseudostellariae is characterized in that may further comprise the steps:
(1) with after pulverizing of Radix Pseudostellariae rhizome or the pelletizing, immerses alcoholic solution;
(2) with Radix Pseudostellariae solution mixture ultrasound-enhanced extraction under water-bath of step (1);
(3) with the ultrasonic extracting liquid filtering that obtains of step (2), and decompression and solvent recovery, be concentrated into the thick paste shape, obtain Radix Pseudostellariae extract.
2. the method for functional component is characterized in that in the supersound extraction Radix Pseudostellariae according to claim 1, and in the step (1), the concentration of alcoholic solution is 15%～95%, and addition is 4～16 ml/g Radix Pseudostellariaes.
3. the method for functional component in the supersound extraction Radix Pseudostellariae according to claim 1 and 2 is characterized in that: supersonic frequency is 20kHZ～100kHz in the described step (2), and intensity is 0.1～0.5W/cm
2
4. the method for supersound extraction Radix Pseudostellariae functional component according to claim 3 is characterized in that: bath temperature is 25 ℃～65 ℃ in the described step (2), and extraction time is 10 min～100min.
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| CN 201110185905 CN102293810A (en) | 2011-07-05 | 2011-07-05 | Pseudostellaria root functional component extraction method by ultrasonic |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104173225A (en) * | 2014-05-21 | 2014-12-03 | 厦门医学高等专科学校 | Skin care product by using radix pseudostellariae as active component and preparation method of skin care product by using radix pseudostellariae as active component |
| CN106176882A (en) * | 2016-08-30 | 2016-12-07 | 福建贝迪药业有限公司 | A kind of preparation method of Radix Pseudostellariae extract |
| CN106214725A (en) * | 2016-08-30 | 2016-12-14 | 福建贝迪药业有限公司 | A kind of preparation method of Radix Pseudostellariae palpus extract |
| CN108841550A (en) * | 2018-09-19 | 2018-11-20 | 福建农林大学 | A kind of processing method that radix pseudostellariae prepares yellow rice wine |
| CN110483615A (en) * | 2019-07-18 | 2019-11-22 | 宁德师范学院 | The extracting method of Radix pseudostellariae cyclic peptides is extracted using surfactant |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1435270A (en) * | 2002-01-31 | 2003-08-13 | 葛松山 | Ultrasonic extracting system for solid-liquid extraction |
| CN101480472A (en) * | 2008-01-09 | 2009-07-15 | 石河子大学 | Medicament for reducing blood pressure and regulating blood fat and production method |
| CN101919561A (en) * | 2009-10-26 | 2010-12-22 | 福建中医学院 | Preparation method of health-care heterophylly faalsestarwort root beverage |
-
2011
- 2011-07-05 CN CN 201110185905 patent/CN102293810A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1435270A (en) * | 2002-01-31 | 2003-08-13 | 葛松山 | Ultrasonic extracting system for solid-liquid extraction |
| CN101480472A (en) * | 2008-01-09 | 2009-07-15 | 石河子大学 | Medicament for reducing blood pressure and regulating blood fat and production method |
| CN101919561A (en) * | 2009-10-26 | 2010-12-22 | 福建中医学院 | Preparation method of health-care heterophylly faalsestarwort root beverage |
Non-Patent Citations (4)
| Title |
|---|
| 《2010中药炮制技术、学术交流暨产业发展高峰论坛论文集》 20101031 王文凯等 中药太子参近年研究概况 103-108，第105页4.2皂苷含量检测 1-4 , * |
| 《中草药》 20010925 许茜等 太子参皂苷提取工艺优选 799-800，第800页右栏第1-4行、3.2第1-4行 1-4 第32卷, 第9期 * |
| 《人参研究》 20100915 秦汝兰 太子参多糖的提取及抗疲劳作用的研究 18-19 1-4 , 第3期 * |
| 《现代中药研究与实践》 20051230 闫亮等 太子参多糖及皂苷的积累动态研究 10-13 1-4 第19卷, 第6期 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104173225A (en) * | 2014-05-21 | 2014-12-03 | 厦门医学高等专科学校 | Skin care product by using radix pseudostellariae as active component and preparation method of skin care product by using radix pseudostellariae as active component |
| CN106176882A (en) * | 2016-08-30 | 2016-12-07 | 福建贝迪药业有限公司 | A kind of preparation method of Radix Pseudostellariae extract |
| CN106214725A (en) * | 2016-08-30 | 2016-12-14 | 福建贝迪药业有限公司 | A kind of preparation method of Radix Pseudostellariae palpus extract |
| CN108841550A (en) * | 2018-09-19 | 2018-11-20 | 福建农林大学 | A kind of processing method that radix pseudostellariae prepares yellow rice wine |
| CN110483615A (en) * | 2019-07-18 | 2019-11-22 | 宁德师范学院 | The extracting method of Radix pseudostellariae cyclic peptides is extracted using surfactant |
| CN110483615B (en) * | 2019-07-18 | 2021-06-15 | 宁德师范学院 | Method for extracting radix pseudostellariae cyclic peptide by using surfactant |
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