CN107982305A - A kind of method of steam explosion assisted extraction astragaloside and astragalus polyose - Google Patents

A kind of method of steam explosion assisted extraction astragaloside and astragalus polyose Download PDF

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Publication number
CN107982305A
CN107982305A CN201610949795.XA CN201610949795A CN107982305A CN 107982305 A CN107982305 A CN 107982305A CN 201610949795 A CN201610949795 A CN 201610949795A CN 107982305 A CN107982305 A CN 107982305A
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astragaloside
steam explosion
steam
explosion
extraction
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不公告发明人
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Beijing Zhongke Bairuineng Engineering Technology Co Ltd
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Beijing Zhongke Bairuineng Engineering Technology Co Ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/48Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/481Astragalus (milkvetch)
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08BPOLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0003General processes for their isolation or fractionation, e.g. purification or extraction from biomass
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30Extraction of the material
    • A61K2236/33Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50Methods involving additional extraction steps
    • A61K2236/53Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization

Abstract

In order to fundamentally release influence of the radix astragali cell membrane compact texture to its active ingredient dissolution, the dissolution efficiency of Astragalus Root P.E is improved, the invention discloses a kind of method of steam explosion assisted extraction astragaloside and polysaccharide:Put into after Milkvetch Root rehydration in steam-explosion jar, be passed through saturated vapor and maintain certain pressure, the release of pressure release after a certain period of time;Raw material after collection steam explosion, is extracted, the astragaloside and polysaccharide that Aqueous extracts are obtained by ethanol precipitation separation and Extraction again with hot water, and precipitation and separation of the supernatant obtain astragalus polyose and astragaloside respectively after purification.This method pre-processes steam explosion technology for radix astragali, material hemicellulose degradation, lignin is softened and is partly dissolved and fibre structure is loose, effectively destroy radix astragali cell membrane and form obvious loose structure, improve the dissolution rate of active ingredient but do not destroy constituent structure and activity.Compared with routinely crushing extraction, 2.8 times and 4.5 times of highest has been respectively increased in astragaloside and astragalus polyose yield, and extraction time shortens at least 1/2 times.

Description

A kind of method of steam explosion assisted extraction astragaloside and astragalus polyose
Technical field
The invention belongs to Technology of Processing Chinese Herbal Medicines field, more particularly to a kind of steam explosion assisted extraction astragaloside and radix astragali are more The method of sugar.
Background technology
Radix astragali, first recorded in《Sheng Nong's herbal classic》, a kind of common pulse family Astragalus, perennial herb Chinese medicine, originate in northeast, The ground such as North China, Shanxi, Gansu, with astragalus mongolicus Astragalus membranaceus (Fisch.) Bge. of cultivation Var. mongholicus (Bge.) Hsiao and Astragalus membranacus A. membranceus (Fisch.) Bge. plant to be former Species matter.For the medicinal history of radix astragali in all age up to more than 2,000 years, the medical value of its underground main root is mainly reflected in tonifying Qi Gu table, sharp water torr sore, cardiac stimulant decompression etc., are widely used in clinic etc., radix astragali mainly contain polysaccharide, a variety of triterpenoid saponins and Flavonoid substances.Astragalus polyose component I, II, III and heteroglycan AH-1, AH-2, having strengthens immunocompetent bioactivity, Including regulation and control immune system, influence human body energy metabolism and the synthesis and metabolism of collagen, protection cell hypoxic-ischemic, suppression Tumour, the pharmacological activity to hemopoietic system etc. act on;Saponin component is with astragaloside IV(Also referred to as Astragaloside IV)And based on II Component is wanted, it is similar with the effect of astragalus polyose, there is decompression, diuresis and cardiac effect, but it also has cardiovascular system Certain influence, improves the effect such as heart contraction and diastole..Structural component in Milkvetch Root(Cellulose, hemicellulose and wood Quality)Content accounts for the 70% ~ 80% of gross mass, and astragaloside and polyoses content are only about 2%, its extract must pull against largely from Tissue, cell are so that structural barriers to fine and close cell membrane level.Therefore, the compact texture of Milkvetch Root how is effectively broken through Barrier, so as to increase extract component and the accessibility of solvent, and then promotes effective dissolution of medicinal ingredient, is to improve in radix astragali to live The basic solution route of property component dissolution rate.
Conventional method generally carries out Milkvetch Root using mechanical crushing broken pretreatment, but due to the compact texture of radix astragali There is certain mechanical resistance, therefore mechanical treatment is limited to the extent of the destruction of its plant cell, there are effective constituents extraction efficiency Low phenomenon;If radix astragali is crushed to powdered, then it can increase energy consumption, improve extraction cost.Steam explosion technology is wide in recent years The general preconditioning technique applied to the refining of lignocellulose-like biomass raw material, it is deep into material using high-temperature high-pressure steam In the hole and cell of tissue, then moment pressure release release material, caused machinery force of explosion can destroy the densification of material Structure, so that using chemical hydrolysis and physical crushing method in the short time(A few minutes)It is interior horizontal effectively from cell or even cell membrane Material is destroyed, loose structure is significantly improved, makes material void volume and aperture increases, rapidly entering and increase beneficial to solvent The accessibility of solubilizer-solute, promotes the dissolution of active ingredient.
The content of the invention
Goal of the invention:Fundamentally to release influence of the radix astragali compact texture to its active ingredient dissolution, and cause effectively Constituents extraction takes long and extracts incomplete problem, proposes a kind of side of steam explosion assisted extraction astragaloside and astragalus polyose Method.
Its specific technical solution is:1)Milkvetch Root is cut to 3 ~ 5 cm, according to the matter of over dry Milkvetch Root and water Measure ratio 1:0~1:4 add water, and rehydration is then soaked under room temperature again handles 0 ~ 12 h;2)Material after rehydration is placed in steam-explosion jar, Being passed through steam makes pressure inside the tank reach the MPa of 0.6 MPa ~ 1.8, and steam explosion processing is carried out after maintaining 1 ~ 15 min;3)By thing after steam explosion 60 DEG C ~ 100 DEG C water of 5 ~ 20 times of material plus its absolute dry mass, are extracted 1 ~ 3 time, 1 ~ 2 h, merges Aqueous extracts every time;4)By Aqueous extracts Ethanol is added after reduction vaporization makes alcohol content reach 50% ~ 100%, while produces precipitation, and sediment fraction and supernatant pass through separation Polysaccharide and saponin constituent is made in purifying respectively.
With patent CN105412209A, CN105343153A, CN105175573A, CN104792652A, CN104530170A、CN104311689A、CN105440147A、CN102875688A、CN104926951A、 CN102697838A、CN102697839A、CN103613674A、CN105440148A、CN103288975A、 CN105399852A、CN105440149A、CN1631904、CN1557826、CN1330082、CN102146142A、 CN101669577、CN1510143、CN102887963A、CN103288974A、CN103110689A、CN102727566A、 CN104107211A、CN102488742A、CN103623039A、CN102101880A、CN104739920A、CN104784248A Etc. comparing, they are in the processing of raw material or convection drying crushes, or are crushed by microwave, or add acid-base reagent and adjust pH, Or complex enzyme zymohydrolysis is added, these pretreatments are limited to the extent of the destruction of plant cell and can increase energy consumption, improve extraction cost It is and complex for operation step etc..
The present invention technical advantage be:1)Under high temperature and pressure effect, vapor enters material tissue voids and iuntercellular, Then moment high speed pay-out comes out, and effectively destroys Milkvetch Root structure from tissue, cell or even cell membrane are horizontal, fundamentally solves Except influence of the radix astragali densification multilevel hierarchy to active ingredient dissolution, the dissolution efficiency of raising astragaloside and polysaccharide;2)Through steam explosion The radix astragali short texture of pretreatment, porosity characteristic are obviously improved, porosity, pore volume and aperture increase, and inside configuration is tortuous Degree reduces, and is conducive to increase the accessibility of solvent-solute and reduces the resistance to mass tranfer of solutes accumulation but do not damage having in raw material Component is imitated, the extracted amount highest of astragaloside and astragalus polyose improves 2.8 times and 4.5 times after steam explosion, and extraction time shortens At least half;3)Any chemical reagents are added without during steam explosion, do not produce pollution and noxious material, are conducive to radix astragali medicine Material contributes to the further industrialization development of Milkvetch Root to utilize in the application in medicines and health protection field;4)With conventional material powder Broken to compare, steam explosion processing destroys raw material internal structure from tissue, cell and cell membrane level, causes the drop of solutes accumulation resistance It is low, and pulverization process substantially has no effect the microstructure of material.
Embodiment
Embodiment 1:
Milkvetch Root is cut to 3 ~ 5 cm, according to over dry Milkvetch Root and the mass ratio 1 of water:1 adds water, then again under room temperature Soak rehydration and handle 4 h;Material after rehydration is placed in steam-explosion jar, being passed through steam makes pressure inside the tank reach 1.2 MPa, maintains pressure Steam explosion processing is carried out after strong 1.2 MPa, 5 min;Material after steam explosion adds to 90 DEG C of water of 10 times of its absolute dry mass, extracts 1 time, often Secondary 1 h, merges Aqueous extracts;Ethanol is added after Aqueous extracts are evaporated under reduced pressure makes alcohol content reach 80%, while produces precipitation, precipitates Polysaccharide and saponin constituent is made by isolating and purifying in part and supernatant respectively.The porosity of radix astragali material, pore volume after steam explosion Be 68.88%, 1.473% and 10332 nm with average pore size, and original non-steam explosion Astragalus membranaceus raw material for 48.43%, 0.629 ml/g and 272.6 nm, astragaloside and polysaccharide extract rate respectively reach 3.697 mg/g and 0.537 g/g after steam explosion, and original non-steam explosion Astragalus membranaceus raw material is 1.597 mg/g and 0.119 g/g.
Embodiment 2:
Milkvetch Root is cut to 3 ~ 5 cm, according to over dry Milkvetch Root and the mass ratio 1 of water:4 add water, then again under room temperature Soak rehydration and handle 12 h;Material after rehydration is placed in steam-explosion jar, being passed through steam makes pressure inside the tank reach 1.8 MPa, maintains Steam explosion processing is carried out after 15 min;Material after steam explosion is added to 100 DEG C of water of 20 times of its absolute dry mass, is extracted 3 times, 2 h, is closed every time And Aqueous extracts;Absolute ethyl alcohol will be added after Aqueous extracts evaporated under reduced pressure, while produce precipitation, sediment fraction and supernatant pass through separation Polysaccharide and saponin constituent is made in purifying respectively.After steam explosion the porosity of radix astragali material, pore volume and average pore size for 70.17%, 2.072 ml/g and 41265 nm, and original non-steam explosion Astragalus membranaceus raw material is 48.43%, 0.629 ml/g and 272.6 nm, after steam explosion Astragaloside and polysaccharide extract rate respectively reach 4.499 mg/g and 0.159 g/g, and original non-steam explosion Astragalus membranaceus raw material is 1.597 Mg/g and 0.119 g/g.
Embodiment 3:
Milkvetch Root is cut to 3 ~ 5 cm, is placed according to Milkvetch Root in steam-explosion jar, being passed through steam makes pressure inside the tank reach 0.6 MPa, steam explosion processing is carried out after maintaining 1 min;Material after steam explosion adds to 60 DEG C of water of 5 times of its absolute dry mass, extracts 1 time, every time 1 H, merges Aqueous extracts;Ethanol is added after Aqueous extracts are evaporated under reduced pressure makes alcohol content reach 50%, while produces precipitation, sediment fraction Polysaccharide and saponin constituent are made respectively by isolating and purifying with supernatant.The porosity of radix astragali material, pore volume peace after steam explosion Equal aperture be 49.03%, 0.701 ml/g and 1913 nm, and original non-steam explosion Astragalus membranaceus raw material be 48.43%, 0.629 ml/g and 272.6 nm, astragaloside and polysaccharide extract rate respectively reach 2.168 mg/g and 0.317 g/g after steam explosion, and original non-steam explosion Astragalus membranaceus raw material is 1.597 mg/g and 0.119 g/g.

Claims (1)

1. a kind of method of steam explosion assisted extraction astragaloside and astragalus polyose, its specific steps include:
(1)Milkvetch Root is cut to 3 ~ 5 cm, according to over dry Milkvetch Root and the mass ratio 1 of water:0~1:4 add water, then Rehydration is soaked under room temperature again and handles 0 ~ 12 h;
(2)Material after rehydration is placed in steam-explosion jar, being passed through steam makes pressure inside the tank reach the MPa of 0.6 MPa ~ 1.8, maintain 1 ~ Steam explosion processing is carried out after 15 min;
(3)Material after steam explosion is added to 60 DEG C ~ 100 DEG C water of 5 ~ 20 times of its absolute dry mass, is extracted 1 ~ 3 time, 1 ~ 2 h, merges every time Aqueous extracts;
(4)Ethanol is added after Aqueous extracts are evaporated under reduced pressure makes alcohol content reach 50% ~ 100%, while produces precipitation, sediment fraction with Polysaccharide and saponin constituent is made by isolating and purifying in supernatant respectively.
CN201610949795.XA 2016-10-27 2016-10-27 A kind of method of steam explosion assisted extraction astragaloside and astragalus polyose Pending CN107982305A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108341892A (en) * 2018-05-09 2018-07-31 四川岚晟生物科技有限公司 A kind of preparation method of Inonotue obliquus
CN108752490A (en) * 2018-05-09 2018-11-06 四川岚晟生物科技有限公司 A kind of preparation method of lentinan
CN110916241A (en) * 2019-11-20 2020-03-27 许达勇 Nicotine aerosol and preparation method thereof
CN111419943A (en) * 2020-05-18 2020-07-17 南京大方生物工程有限公司 Antiviral traditional Chinese medicine Qilanbao disease drink for livestock, preparation method and application
CN112778438A (en) * 2021-01-26 2021-05-11 华南农业大学 Method for improving pachyman extraction rate by steam explosion and enzymolysis technology

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101401825A (en) * 2008-11-11 2009-04-08 中国科学院过程工程研究所 Method for steam explosion processing process for single and compound traditional Chinese medicine
CN102443072A (en) * 2010-10-12 2012-05-09 沈阳药科大学 Method for simultaneously extracting polysaccharides and saponins from astragalus
CN104585462A (en) * 2015-02-10 2015-05-06 寿素贞 Method for preparing preserved fruits capable of enhancing body immunologic function from astragalus membranaceus

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101401825A (en) * 2008-11-11 2009-04-08 中国科学院过程工程研究所 Method for steam explosion processing process for single and compound traditional Chinese medicine
CN102443072A (en) * 2010-10-12 2012-05-09 沈阳药科大学 Method for simultaneously extracting polysaccharides and saponins from astragalus
CN104585462A (en) * 2015-02-10 2015-05-06 寿素贞 Method for preparing preserved fruits capable of enhancing body immunologic function from astragalus membranaceus

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108341892A (en) * 2018-05-09 2018-07-31 四川岚晟生物科技有限公司 A kind of preparation method of Inonotue obliquus
CN108752490A (en) * 2018-05-09 2018-11-06 四川岚晟生物科技有限公司 A kind of preparation method of lentinan
CN110916241A (en) * 2019-11-20 2020-03-27 许达勇 Nicotine aerosol and preparation method thereof
CN111419943A (en) * 2020-05-18 2020-07-17 南京大方生物工程有限公司 Antiviral traditional Chinese medicine Qilanbao disease drink for livestock, preparation method and application
CN112778438A (en) * 2021-01-26 2021-05-11 华南农业大学 Method for improving pachyman extraction rate by steam explosion and enzymolysis technology

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