CN106174550A - A kind of pitaya peel effervescent tablet - Google Patents
A kind of pitaya peel effervescent tablet Download PDFInfo
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- CN106174550A CN106174550A CN201610575140.0A CN201610575140A CN106174550A CN 106174550 A CN106174550 A CN 106174550A CN 201610575140 A CN201610575140 A CN 201610575140A CN 106174550 A CN106174550 A CN 106174550A
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Abstract
A kind of pitaya peel effervescent tablet, described effervescent tablet is made up of the component of following parts by weight: pitaya peel dry powder 80～100 parts, Caulis Bambusae In Taenia 10～20 parts, sugarcane wax 5～10 parts, carotene 5～10 parts, sodium bicarbonate 50～60 parts, edible calcium carbonate 1～2 parts, sodium dihydrogen phosphate 1～5 parts, malic acid 30～50 parts, Rhizoma Steudnerae Henryanae mud 30～50 parts, sucrose 30～50 parts and edible activated carbon 5～10 parts.The present invention prepares effervescent tablet with the pitaya peel rich in anthocyanidin for raw material, both can be that consumer provides diversified nutrition product, also solve the problem that the problem that the direct eating mouth feel of pitaya peel is the best.The present invention with pitaya peel as major ingredient, the functional components such as compatibility edible activated carbon, Caulis Bambusae In Taenia and sugarcane wax, for defying age, there is synergism.
Description
Technical field
The present invention relates to food processing technology field, specifically a kind of pitaya peel effervescent tablet.
Background technology
Effervescent tablet is a kind of novel tablet of Abroad in Recent Years exploitation application.Its difference with conventional tablet, just exists
In it possibly together with gas-producing disintegrant, after effervescent tablet is put in drinking-water, under the effect of gas-producing disintegrant, at once produce a large amount of
Bubble, makes the rapid disintegrate of tablet and thawing, and the bubble that disintegrate sometimes produces also can make tablet roll up and down in water, accelerates it and collapses
Solve and melt.The gas produced during disintegration of tablet is partially dissolved in drinking-water, makes drinking-water have the aesthetic feeling as soda pop when drinking in entrance.
Effervescent tablet has following advantage: be easy to preserve and carry；Disintegrate quickly, taking convenience, onset rapid；Bioavailability is high, energy
Improve clinical efficacy；It is particularly well-suited to child, old people and swallows the patient of pill difficulty；Through seasoned effervescent tablet,
More preferably, good medicine is the most bitter to the taste for taste, makes patient more be willing to accept.
The peel of Hylocereus undatus contains the nutrient substance anthocyanidin being of great rarity.Anthocyanidin is the antioxidation of a kind of strength
Agent, can preserve activity 75 hours in blood of human body.It can protect human body from the damage of harmful substance free radical, has
Help prevent the multiple disease relevant with free radical.Anthocyanidin can strengthen blood vessel elasticity, protects arteries inwall；Reduce blood
Pressure；Promote the smoothness of skin, U.S. face skin；Suppression inflammation and allergy, improve the pliability in joint, prevents arthritis；Permissible
Promote the rhodopsin regeneration in retina cell, improve vision；Also there is radiation-resistant effect etc..Anthocyanidin is tieed up in terms of many
Protect the health of human body, bring multiple benefit for us.
Summary of the invention
Present invention aim at the increment utilization for pitaya peel and human nutrition material demand, it is provided that a kind of Hylocereus undatus
Skin effervescent tablet.
Technical scheme is as follows:
A kind of pitaya peel effervescent tablet, described effervescent tablet is made up of the component of following parts by weight:
Pitaya peel dry powder 80～100 parts, Caulis Bambusae In Taenia 10～20 parts, sugarcane wax 5～10 parts, carotene 5～10 parts, carbonic acid
Hydrogen sodium 50～60 parts, edible calcium carbonate 1～2 parts, sodium dihydrogen phosphate 1～5 parts, malic acid 30～50 parts, Rhizoma Steudnerae Henryanae mud 30～50 parts,
Sucrose 30～50 parts and edible activated carbon 5～10 parts.
The preparation method of described pitaya peel dry powder is as follows:
Select fresh mature without the Hylocereus undatus of pest and disease damage, clean up rear peeling, obtain pitaya peel；Pitaya peel is put
Enter in the water of 90～100 DEG C drift soup enzyme denaturing, the water of drift soup is 0.05 containing percentage by weight～the sodium alginate of 0.1%,
0.05～the lysine of 0.1% and 0.1～the tea polyphenols of 0.2%；After quickly draining, in ultrasonic cleaning high pressure environment, quick-freezing is to subzero
15～30 DEG C, supersonic frequency is 25～40Hz, and ultrasonic power density is 0.35～0.45w/cm2, quick-freezing environmental air pressure is
300～350Mpa；The most under freezing conditions with disintegrating apparatus, material is broken into the granularity fragment less than 0.5 centimetre, then puts
Putting and dry in vacuum drying oven to water content less than 5 percent, in vacuum drying oven, temperature is 65～75 DEG C, vacuum be 70～
85kPa；Last micronizing again obtains pitaya peel dry powder.
Beneficial effects of the present invention is as follows:
(1) prepare effervescent tablet with the pitaya peel rich in anthocyanidin for raw material, both can be that consumer provides diversified battalion
Support product, also solve the problem that the problem that the direct eating mouth feel of pitaya peel is the best.
(2) present invention is with pitaya peel as major ingredient, the functional components such as compatibility Caulis Bambusae In Taenia and sugarcane wax, has for defying age
Synergism.
(3) and preparation technology is simple, it is easy to preserve, effect duration is long, is unlikely to deteriorate and is easily mastered dosage, be suitable for multiple
The plant produced of scale, has huge commercial promise.
Detailed description of the invention
Further describe the present invention below by way of specific embodiment, but be not limited to this, can weigh in the present invention
Profit requires to carry out various change in the range of restriction.
Embodiment 1
A kind of pitaya peel effervescent tablet, described effervescent tablet is made up of the component of following parts by weight:
90 parts of pitaya peel dry powder, Caulis Bambusae In Taenia 15 parts, sugarcane wax 8 parts, carotene 8 parts, sodium bicarbonate 55 parts, edible carbonic acid
Calcium 1.5 parts, sodium dihydrogen phosphate 2 parts, malic acid 40 parts, 40 parts of Rhizoma Steudnerae Henryanae mud, sucrose 40 parts and edible activated carbon 8 parts.
The preparation method of described pitaya peel dry powder is as follows:
Select fresh mature without the Hylocereus undatus of pest and disease damage, clean up rear peeling, obtain pitaya peel；Pitaya peel is put
Enter drift soup enzyme denaturing in the water of 95 DEG C, containing sodium alginate, the bad ammonia of 0.08% that percentage by weight is 0.08% in the water of drift soup
Acid and the tea polyphenols of 0.15%；After quickly draining, in ultrasonic cleaning high pressure environment, quick-freezing is to subzero 20 DEG C, and supersonic frequency is 30Hz,
Ultrasonic power density is 0.40w/cm2, quick-freezing environmental air pressure is 320Mpa；The most under freezing conditions with disintegrating apparatus by thing
Material is broken into the granularity fragment less than 0.5 centimetre, is then placed within vacuum drying oven drying to water content less than 5 percent, very
In empty baking oven, temperature is 70 DEG C, and vacuum is 75kPa；Last micronizing again obtains pitaya peel dry powder.
Pitaya peel effervescent tablet described in the present embodiment is prepared by direct compression after raw material components mix homogeneously.
The functional Quality Research of pitaya peel dry powder
The determination of total flavonoids of the pitaya peel dry powder of the present embodiment gained
1.0g fruit powder takes supernatant after 10mL80% ethanol dissolves after 10000r/min is centrifuged 10min, measures fruit powder
General flavone content.100.0 μ L extracting solution, in 10mL plastic centrifuge tube, successively add 0.3mL8%NaNO2, reaction 6min,
0.3mL10%Al (NO3)3, reaction 6min, 2.0mL2mol/LNaOH and 4.9mL ethanol, mixing stand 10min.Then
Under 10000r/min, centrifugal 10min, collects supernatant, with 80% ethanol for comparison, with the suction at spectrophotometric determination 510nm
Light value, calculates general flavone content, in units of the rutin equivalent of mg/g fruit powder.Repeat to test 3 times, average.
The Vc assay of the pitaya peel dry powder of the present embodiment gained
Weigh after 3g sample fully dissolves, constant volume centrifugal 5min under 4000r/min after 50mL volumetric flask, take supernatant and survey
Determine Vc content.Take 0.2mL extracting solution to put in the 10mL volumetric flask filling 2mL10% hydrochloric acid, shake to scale with distilled water diluting
Even, with distilled water as blank, at 245nm, measure the light absorption value of sample.Respectively draw 0.2mL extracting solution, 2mL distilled water and
0.8mL1mol/LNaOH is sequentially placed in 10mL volumetric flask, mixing, adds 0.8mL10% hydrochloric acid, mixing, constant volume after 15min.
With distilled water as blank, at 254nm, measure the light absorption value of alkali treatment fluid.The extinction value processed by testing sample and alkali to be measured it
Difference and ascorbic acid standard curve, calculate the Vc content (mg/g) in sample.Repeat, average for 3 times.
Total flavones in the different pitaya peel dry powder of table 1 and the comparison of Vc content
| Total flavones (mg/g) | Vc content (mg/g) | |
| The present embodiment | 71.55±2.13 | 0.44±0.08 |
| Room temperature enzymolysis (ET) | 68.58±1.15 | 0.46±0.05 |
| Heating enzymolysis (HE) | 68.81±3.82 | 0.34±0.09 |
| Non-enzymolysis (CK) | 70.45±4.51 | 0.40±0.08 |
Total flavones and Vc are nutritional labelings important in fruit powder, are fruit powder antioxidative effective ingredient, and different disposal is to fire
The impact of dragon fruit peel fruit powder total flavones and Vc content is bigger.From table 1 it follows that fruit powder preparation process is carried out heat enzyme
Processing thus reduce Vc content in sample, in heat treated sample, Vc content (0.34mg/g) is less than not carrying out ferment treatment and room temperature
Ferment treatment sample, respectively 0.40,0.46mg/g, and the present embodiment gained pitaya peel dry powder Vc content is close to room temperature enzymolysis
Vc content, explanation the method for the invention prepares pitaya peel dry powder, and Vc loss is less；Through ferment treatment and heating ferment treatment
In fruit powder, general flavone content to be less than the most enzyme treated sample, illustrates that the high temperature in spray-drying process and pectinase treatment can drop
General flavone content in low Hylocereus undatus peel, and the general flavone content of the present embodiment gained pitaya peel dry powder is higher than other three groups pairs
Ratio, the total flavones loss that the method for the invention causes is less, and the superiority of the inventive method is described, this is likely due to this
Invent the temperature of dry run is relatively low and the present invention does not has pulping process, and the physical damage process of making beating causes nutrient substance
Lose more, but utilize quick-freezing to destroy pericarp tissue so that the nutritional labeling in peel keeps preferably.
The Antioxidative Activity Determination of the pitaya peel dry powder of the present embodiment gained
(1) FRAP method
Take 100.0 μ L extracting solution and 900.0 μ LFe3+-TPTZ reactant liquor (includes 0.3mmol/L acetate buffer
25.0mL, 10.0mmol/LTPTZ solution 2.5mL, 20.0mmol/LFeCl3 solution 2.5mL) mixing, react 10min, with 80%
Ethanol is comparison, and Trolox is standard, with the light absorption value at spectrophotometric determination 595nm, calculates antioxidant activity, with
Mmol/g fruit powder, trolox equivalent (TEAC) are unit.Repeat, average for 3 times.
(2) DPPH method
Take 100.0 μ L extracting solution to mix with 3.9mLDPPH (6.0 μm ol/L), react 6h, with 80% ethanol for comparison,
Trolox is standard, and with the light absorption value at spectrophotometric determination 515nm, (DPPH* is clear to calculate antioxidant activity as follows
Except rate), in units of TEAC.DPPH* clearance rate=(A0-A1)×100/A0(A0Represent blank, A1Representative sample detected value).3
Secondary repetition, averages.
The oxidation resistance of the different pitaya peel dry powder of table 2 compares
| FRAP value (μm ol/100g) | DPPH value (μm ol/100g) | |
| The present embodiment | 1.85±0.10 | 5.32±0.05 |
| Room temperature enzymolysis (ET) | 1.69±0.19 | 4.91±0.08 |
| Heating enzymolysis (HE) | 0.64±0.04 | 3.68±0.08 |
| Non-enzymolysis (CK) | 1.91±0.08 | 5.25±0.11 |
FRAP value and DPPH value are two kinds of widely used non-oxidizability evaluation methodologys, and wherein DPPH method is at organic solvent
The ability of the removing DPPH free radical of middle mensuration compound, and FRAP is in the solution of low ph value, Fe3+It is reduced to by antioxidant
Fe2+.From Table 2, it can be seen that the FRAP value of the pitaya peel dry powder prepared by the method for the invention is higher than heating enzymolysis
(HE) and the FRAP value of room temperature enzymolysis (ET), and close to the FRAP value of non-enzymolysis (CK)；And prepare by the method for the invention
The DPPH value of pitaya peel dry powder is higher than other DPPH values of three groups.Illustrating that the inventive method prepares pitaya peel dry powder can be relatively
Keep nutritional labeling well.
In Tables 1 and 2, the preparation method of three groups of comparative examples is as follows: Hylocereus undatus peel is divided into 3 parts after making beating, its
In 1 part without enzymolysis (CK), under 1 part of room temperature, (20 DEG C) carry out ferment treatment 1h (ET) with pectase, and the 3rd part is heated to when 50 DEG C entering
Row pectinase treatment 1h (HE).Pulp is spray-dried after colloid mill, concentration, homogenizing, becomes fruit powder.
The research of pitaya peel effervescent tablet anti-aging effects
The Kunming mouse of 48 6 week old body weight 23～27g is randomly divided into 6 groups, often 8 (each 4 of male and female) of group, respectively
For agent in blank group, modeling group, commercially available vitamin C effervescent tablet group, pitaya peel effervescent tablet low dose group, pitaya peel effervescent tablet
Amount group and pitaya peel effervescent tablet high dose group, successive administration 60d.
Blank group: every day feeding Rhizoma Steudnerae Henryanae mud 4g,
Modeling group: every day feeding Rhizoma Steudnerae Henryanae mud 4g,
Commercially available vitamin C effervescent tablet group: feeding every day commercially available vitamin C effervescent tablet 4g,
Pitaya peel effervescent tablet low dose group: the pitaya peel effervescent tablet low dosage 3g that every day, feeding the present embodiment prepared,
Dosage group in pitaya peel effervescent tablet: the pitaya peel effervescent tablet low dosage 4g that every day, feeding the present embodiment prepared,
Pitaya peel effervescent tablet high dose group: the pitaya peel effervescent tablet low dosage 5g that every day, feeding the present embodiment prepared.
(1) after last is administered 24h, claiming Mouse Weight, eye socket puts to death mice after taking blood, takes thymus, spleen, liver and brain group
Knit and weigh, measuring index of correlation respectively.Calculate thymus by " thymus (mg)/weight (g), spleen (mg)/weight (g) " to refer to
Number and index and spleen index.
(2) by mouse blood its serum of centrifuging and taking immediately, operate by Malondialdehyde Kit description, measure at 532nm
Each pipe absorbance, calculates mda content MDA in serum.
Operate by superoxide dismutase test kit description.At 550nm, measure each pipe absorbance, calculate in serum super
Superoxide dismutase vigor SOD.
(3) taking mouse liver, remove connective tissue, homogenizer is ground, is added the normal saline of pre-cooling and is prepared as the group of 10%
Knit homogenate.Frozen centrifugation (2 DEG C～4 DEG C), takes its supernatant, by glutathion peroxidase kit measurement glutathion mistake
Oxide enzyme activity GSH-Px.
(4) taking Mice brain tissues, the normal saline adding pre-cooling carries out being homogenized the tissue homogenate being prepared as 10%.Frozen centrifugation
(2 DEG C～4 DEG C), take its supernatant, measure activity of catalase CAT with hydroperoxidase reagent box.
Experimental data mean represents, compares employing variance analysis between group.
From table 3, comparing with blank group, modeling group Thymus and Spleen index all significantly reduces (P < 0.05)；
Compare with modeling group, the Thymus and Spleen index of commercially available vitamin C effervescent tablet group have notable rising (P <
0.05)；Dosage group and pitaya peel effervescent tablet high dose group in pitaya peel effervescent tablet low dose group, pitaya peel effervescent tablet
Thymus and Spleen index all has highly significant to raise (P < 0.01).
The result of the test of table 3 Thymus and Spleen index
Note: for blank group,*P<0.05；For modeling group,#P < 0.05,##P<0.01
From table 4, comparing with blank group, modeling group MDA content has highly significant to raise, SOD, GSH-Px, CAT vigor
Highly significant is had to reduce (P < 0.01)；
Compare with modeling group, commercially available vitamin C effervescent tablet group, pitaya peel effervescent tablet low dose group and pitaya peel effervescent
In sheet, the MDA content of dosage group significantly reduces (P < 0.05), and the MDA content of pitaya peel effervescent tablet high dose group has the most aobvious
Write and reduce (P < 0.01)；
Comparing with modeling group, SOD and CAT of commercially available vitamin C effervescent tablet group is by significantly improving (P < 0.05), and SH-Px lives
Power has clearly raising (P < 0.01)；Dosage group and fire in pitaya peel effervescent tablet low dose group, pitaya peel effervescent tablet
SOD, GSH-Px and CAT vigor of dragon peel effervescent tablet high dose group all has highly significant to improve (P < 0.01).
The result of the test of table 4 MDA, SOD, GSH-Px and CAT
| MDA(μmol/L) | SOD(U/ml) | GSH-Px(U/ml) | CAT(U/ml) | |
| Blank group | 41.1 | 95.6 | 266.8 | 3.81 |
| Modeling group | 47.5** | 63.9** | 146.8** | 2.21** |
| Commercially available vitamin C effervescent tablet group | 44.5# | 78.8# | 316.4## | 3.28# |
| Pitaya peel effervescent tablet low dose group | 43.8# | 90.8## | 310.1## | 3.64## |
| Dosage group in pitaya peel effervescent tablet | 42.3# | 91.3## | 358.3## | 3.96## |
| Pitaya peel effervescent tablet high dose group | 41.7## | 93.1## | 368.1## | 3.88## |
Note: for blank group,**P<0.01；For modeling group,#P < 0.05,##P<0.01。
Embodiment 2
A kind of pitaya peel effervescent tablet, described effervescent tablet is made up of the component of following parts by weight:
100 parts of pitaya peel dry powder, Caulis Bambusae In Taenia 20 parts, sugarcane wax 10 parts, carotene 10 parts, sodium bicarbonate 60 parts, edible carbon
Acid calcium 2 parts, sodium dihydrogen phosphate 5 parts, malic acid 50 parts, 50 parts of Rhizoma Steudnerae Henryanae mud, sucrose 50 parts and edible activated carbon 10 parts.
The preparation method of described pitaya peel dry powder is as follows:
Select fresh mature without the Hylocereus undatus of pest and disease damage, clean up rear peeling, obtain pitaya peel；Pitaya peel is put
Enter drift soup enzyme denaturing in the water of 100 DEG C, containing sodium alginate, the lysine of 0.1% that percentage by weight is 0.1% in the water of drift soup
And the tea polyphenols of 0.2%；After quickly draining, in ultrasonic cleaning high pressure environment, quick-freezing is to subzero 30 DEG C, and supersonic frequency is 40Hz, ultrasonic
Power density is 0.45w/cm2, quick-freezing environmental air pressure is 350Mpa；The most under freezing conditions with disintegrating apparatus, material is broken
Being broken into the granularity fragment less than 0.5 centimetre, be then placed within vacuum drying oven drying to water content less than 5 percent, vacuum is dried
In case, temperature is 75 DEG C, and vacuum is 85kPa；Last micronizing again obtains pitaya peel dry powder.
Embodiment 3
A kind of pitaya peel effervescent tablet, described effervescent tablet is made up of the component of following parts by weight:
80 parts of pitaya peel dry powder, Caulis Bambusae In Taenia 10 parts, sugarcane wax 5 parts, carotene 5 parts, sodium bicarbonate 50 parts, edible carbonic acid
Calcium 1 part, sodium dihydrogen phosphate 1 part, malic acid 30 parts, 30 parts of Rhizoma Steudnerae Henryanae mud, sucrose 30 parts and edible activated carbon 5 parts.
The preparation method of described pitaya peel dry powder is as follows:
Select fresh mature without the Hylocereus undatus of pest and disease damage, clean up rear peeling, obtain pitaya peel；Pitaya peel is put
Enter drift soup enzyme denaturing in the water of 90 DEG C, containing sodium alginate, the bad ammonia of 0.05% that percentage by weight is 0.05% in the water of drift soup
Acid and the tea polyphenols of 0.1%；After quickly draining, in ultrasonic cleaning high pressure environment, quick-freezing is to subzero 15 DEG C, and supersonic frequency is 25Hz, super
Sound power density is 0.35w/cm2, quick-freezing environmental air pressure is 300Mpa；The most under freezing conditions with disintegrating apparatus by material
It is broken into the granularity fragment less than 0.5 centimetre, is then placed within vacuum drying oven drying to water content less than 5 percent, vacuum
In baking oven, temperature is 65 DEG C, and vacuum is 70kPa；Last micronizing again obtains pitaya peel dry powder.
Claims (2)
1. a pitaya peel effervescent tablet, is characterized in that, described effervescent tablet is made up of the component of following parts by weight:
Pitaya peel dry powder 80～100 parts, Caulis Bambusae In Taenia 10～20 parts, sugarcane wax 5～10 parts, carotene 5～10 parts, sodium bicarbonate
50～60 parts, edible calcium carbonate 1～2 parts, sodium dihydrogen phosphate 1～5 parts, malic acid 30～50 parts, Rhizoma Steudnerae Henryanae mud 30～50 parts, sucrose
30～50 parts and edible activated carbon 5～10 parts.
Pitaya peel effervescent tablet the most according to claim 1, is characterized in that, the preparation method of pitaya peel dry powder is as follows:
Select fresh mature without the Hylocereus undatus of pest and disease damage, clean up rear peeling, obtain pitaya peel；Pitaya peel is put into 90
～the water of 100 DEG C floats soup enzyme denaturing, the water of drift soup is 0.05 containing percentage by weight～the sodium alginate of 0.1%, 0.05～
The lysine of 0.1% and 0.1～the tea polyphenols of 0.2%；After quickly draining, in ultrasonic cleaning high pressure environment, quick-freezing is to subzero 15～30
DEG C, supersonic frequency is 25～40Hz, and ultrasonic power density is 0.35～0.45w/cm2, quick-freezing environmental air pressure be 300～
350Mpa；The most under freezing conditions with disintegrating apparatus, material is broken into the granularity fragment less than 0.5 centimetre, is then placed within true
Drying to water content less than 5 percent in empty baking oven, in vacuum drying oven, temperature is 65～75 DEG C, and vacuum is 70～85kPa；
Last micronizing again obtains pitaya peel dry powder.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102366106A (en) * | 2011-10-08 | 2012-03-07 | 昆明理工大学 | Pitaya pericarp dietary fiber powder |
| CN103082376A (en) * | 2013-03-04 | 2013-05-08 | 时振华 | Solid beverage with medlar as raw material and preparation method of solid beverage |
| CN103478331A (en) * | 2013-09-26 | 2014-01-01 | 凌爱秋 | Dragon fruit peel tea and preparation method thereof |
-
2016
- 2016-07-20 CN CN201610575140.0A patent/CN106174550A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102366106A (en) * | 2011-10-08 | 2012-03-07 | 昆明理工大学 | Pitaya pericarp dietary fiber powder |
| CN103082376A (en) * | 2013-03-04 | 2013-05-08 | 时振华 | Solid beverage with medlar as raw material and preparation method of solid beverage |
| CN103478331A (en) * | 2013-09-26 | 2014-01-01 | 凌爱秋 | Dragon fruit peel tea and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| 吴大真等: "《保健食品必读》", 30 September 2009, 中国医药科技出版社 * |
| 西木博士著: "《不生病的饮食起居第2季》", 31 January 2014, 湖北科学技术出版社 * |
| 赵余庆等: "《食疗与保健食品原料功能因子手册》", 31 March 2013, 中国医药科技出版社 * |
| 陈永红等: "《面向未来的食品加工技术》", 31 January 2015, 中国农业科学技术出版社 * |
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Application publication date: 20161207 |
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| RJ01 | Rejection of invention patent application after publication |