CN106170295B - 与通过蛋白诱导的体内细胞重编程的细胞转化相关的方法、系统和组合物 - Google Patents

与通过蛋白诱导的体内细胞重编程的细胞转化相关的方法、系统和组合物 Download PDF

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CN106170295B
CN106170295B CN201480071293.0A CN201480071293A CN106170295B CN 106170295 B CN106170295 B CN 106170295B CN 201480071293 A CN201480071293 A CN 201480071293A CN 106170295 B CN106170295 B CN 106170295B
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modified
protein
protein transduction
cells
reagent
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CN106170295A (zh
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王建军
李倩倩
麦可·乔普
姜丰
吴国俊
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Wayne State University
Qurgen Inc
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Qurgen Inc
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CN201480071293.0A 2013-10-25 2014-10-27 与通过蛋白诱导的体内细胞重编程的细胞转化相关的方法、系统和组合物 Active CN106170295B (zh)

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US201361895562P 2013-10-25 2013-10-25
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US (2) US10076574B2 (https=)
EP (2) EP3954381A1 (https=)
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Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
WO2015105955A1 (en) 2014-01-08 2015-07-16 Flodesign Sonics, Inc. Acoustophoresis device with dual acoustophoretic chamber
JP6530206B2 (ja) * 2015-03-17 2019-06-12 学校法人同志社 上皮間葉転換阻害剤及び癌転移治療剤
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
CN106110297B (zh) * 2016-07-05 2019-12-17 南通大学 Gfi1截短体的应用
WO2018112545A1 (en) * 2016-12-23 2018-06-28 The University Of Queensland Inhibitors of sox18 protein activity for treating angiogenesis- and/or lymphangiogenesis-related diseases
JP2018132365A (ja) * 2017-02-14 2018-08-23 株式会社神戸製鋼所 マーキング装置
IL274926B2 (en) 2017-12-01 2026-04-01 Encoded Therapeutics Inc Pyrazolopyrimidines with activity against respiratory syncytial virus
WO2019118921A1 (en) 2017-12-14 2019-06-20 Flodesign Sonics, Inc. Acoustic transducer drive and controller
US11340226B2 (en) * 2018-03-02 2022-05-24 The Chinese University Of Hong Kong Squalene epoxidase in the diagnosis and treatment of non-alcoholic fatty liver diseases
WO2019168697A1 (en) * 2018-03-02 2019-09-06 Northwestern University Overexpression of foxc1 to treat corneal vascularization
JP7217542B2 (ja) * 2018-03-29 2023-02-03 国立大学法人 琉球大学 分化コントロール化合物を用いて造腫瘍性をもつおそれのある未分化iPS細胞等の混入を除去する方法
US12274733B2 (en) 2018-09-28 2025-04-15 President And Fellows Of Harvard College Cellular reprogramming to reverse aging and promote organ and tissue regeneration
MX2021003663A (es) 2018-09-28 2021-05-28 Harvard College Reprogramacion celular para revertir el envejecimiento y promover la regeneracion de organos y tejidos.
US20220143142A1 (en) * 2019-03-17 2022-05-12 Baylor College Of Medicine Direct reprogramming of cardiac fibroblasts into cardiomyocytes using an endothelial cell transdifferentiation strategy
CN113710285A (zh) * 2019-03-26 2021-11-26 宾州研究基金会 用于治疗癌症的方法和材料
KR102150489B1 (ko) * 2019-04-09 2020-09-01 고려대학교 산학협력단 소변세포로부터 신장전구세포로의 직접 역분화를 유도하는 방법 및 이의 방법으로 역분화된 신장전구세포를 포함하는 신장세포 손상 질환 예방 또는 치료용 약학 조성물
WO2020243651A1 (en) 2019-05-29 2020-12-03 Encoded Therapeutics, Inc. Compositions and methods for selective gene regulation
AU2020368526A1 (en) * 2019-10-17 2022-05-12 The Penn State Research Foundation Regenerating functional neurons for treatment of neurological disorders
CN114729018A (zh) * 2019-10-17 2022-07-08 宾州研究基金会 再生功能神经元以治疗出血性中风
CA3157523A1 (en) * 2019-10-17 2021-04-22 Gong Chen Regenerating functional neurons for treatment of spinal cord injury and als
EP4065227A4 (en) * 2019-11-25 2023-12-20 The Penn State Research Foundation BRAIN REGENERATION AFTER TRAUMATIC BRAIN INJURY BY NEUROD1-MEDIATED ASTROCYTE-TO-NEURON CONVERSION
CN112877364B (zh) * 2019-11-29 2023-07-28 中国医学科学院药物研究所 软骨下骨细胞向关节软骨细胞直接转化的重编程诱导方案
WO2021149823A1 (ja) * 2020-01-24 2021-07-29 アイ ピース, インコーポレイテッド 因子を導入された細胞の培養方法
CN111214660B (zh) * 2020-02-25 2022-01-18 北京大学第三医院(北京大学第三临床医学院) Pax4基因表达抑制剂在制备抑制纤维化的药物中的应用
US11091524B1 (en) 2020-04-22 2021-08-17 Houston Gene Therapeutics Llc Compositions for treatment of vascular disease
CN114231489A (zh) * 2020-09-08 2022-03-25 纽伦捷生物医药科技(苏州)有限公司 用于重编程的功能性片段、组合及其应用
US20220098255A1 (en) * 2020-09-29 2022-03-31 NeuExcell Therapeutics Inc. Neurod1 combination vector
PH12023550859A1 (en) * 2020-09-29 2023-11-20 Neuexcell Therapeutics Inc Dlx2 vector
JP2023543361A (ja) * 2020-09-29 2023-10-13 ニューエクセル・セラピューティクス・インコーポレイテッド Neurod1及びdlx2ベクター
JP2022104813A (ja) 2020-12-29 2022-07-11 アイ ピース,インコーポレイテッド リプログラミング因子を導入された細胞の培養方法
CN113563428B (zh) * 2021-06-22 2022-08-02 呈诺再生医学科技(珠海横琴新区)有限公司 特定空间结构多肽及其在制备iPSC中的应用
CN117070477A (zh) * 2022-05-10 2023-11-17 纽伦捷生物医药科技(上海)有限公司 重组溶瘤病毒的重编程的功能性片段、组合及其应用
KR102902484B1 (ko) * 2022-11-25 2025-12-22 고려대학교 산학협력단 방사선 치료에 의하여 손상된 근육 재생을 위한 고기능성 세포 치료제 조성물
US12252518B2 (en) 2023-01-06 2025-03-18 Life Biosciences, Inc. Methods of treating non-arteritic anterior ischemic optic neuropathy
WO2025106947A1 (en) * 2023-11-17 2025-05-22 Exir, Inc. Compositions and methods for induced stem cell differentiation to neuronal lineage

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090298111A1 (en) * 2008-05-28 2009-12-03 Wayne State University Method and composition for a protein transduction technology and its applications
CN102115760A (zh) * 2009-12-30 2011-07-06 高丽大学校产学协力团 使体细胞重编程以形成诱导全能干细胞的组合物,和用其形成诱导全能干细胞的方法
CN102272142A (zh) * 2008-12-23 2011-12-07 帷幄生物技术公司 非遗传修饰性重编程细胞的组合物和方法
WO2012022725A2 (en) * 2010-08-19 2012-02-23 F. Hoffmann-La Roche Ag Conversion of somatic cells to induced reprogrammed neural stem cells (irnscs)
WO2012151234A2 (en) * 2011-05-02 2012-11-08 Wayne State University A protein-induced pluripotent cell technology uses thereof
WO2013013105A2 (en) * 2011-07-19 2013-01-24 Vivoscript,Inc. Compositions and methods for re-programming cells without genetic modification for repairing cartilage damage

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4666828A (en) 1984-08-15 1987-05-19 The General Hospital Corporation Test for Huntington's disease
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801531A (en) 1985-04-17 1989-01-31 Biotechnology Research Partners, Ltd. Apo AI/CIII genomic polymorphisms predictive of atherosclerosis
US5272057A (en) 1988-10-14 1993-12-21 Georgetown University Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
US5192659A (en) 1989-08-25 1993-03-09 Genetype Ag Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
DE69332485T2 (de) 1992-08-11 2003-11-13 The President And Fellows Of Harvard College, Cambridge Immunmodulierende peptide
FR2766195A1 (fr) 1997-07-21 1999-01-22 Transgene Sa Polymeres cationiques, complexes associant lesdits polymeres cationiques et des substances therapeutiquement actives comprenant au moins une charges negative, notamment des acides nucleiques, et leur utilisation en therapie genique
DE19839491A1 (de) 1998-08-29 2000-03-02 Hermann Heumann Verfahren zur Markierung von Biopolymeren mit Isotopen
US20040142474A1 (en) 2000-09-14 2004-07-22 Expression Genetics, Inc. Novel cationic lipopolymer as a biocompatible gene delivery agent
US6696038B1 (en) 2000-09-14 2004-02-24 Expression Genetics, Inc. Cationic lipopolymer as biocompatible gene delivery agent
ES2315384T3 (es) 2001-07-06 2009-04-01 Merck Patent Gmbh Procedimiento para el seguimiento y la modulacion del plegamiento de proteinas.
EP1448772B1 (en) 2001-11-30 2007-07-18 Applied Research Systems ARS Holding N.V. Methods of increasing expression levels of human tnf receptor
US20030134352A1 (en) 2002-01-04 2003-07-17 Freimuth Paul I. Facilitating protein folding and solubility by use of peptide extensions
WO2003057897A2 (en) 2002-01-07 2003-07-17 European Molecular Biology Laboratory Recombinant protein expression
WO2005026196A2 (en) 2003-09-12 2005-03-24 Novexin Limited Methods for folding proteins and reducing protein aggregation
WO2005051421A2 (en) 2003-11-25 2005-06-09 The Government Of The United States, As Represented By The Secretary Of The Army Use of shigella invaplex to transport functional proteins and transcriptionally active nucleic acids across mammalian cell membranes in vitro and in vivo
RU2450832C2 (ru) 2006-10-25 2012-05-20 Конинклейке Филипс Электроникс Н.В. Контрастные вещества для детекции рака предстательной железы
SG188904A1 (en) 2008-03-17 2013-04-30 Scripps Research Inst Combined chemical and genetic approaches for generation of induced pluripotent stem cells
KR20110134939A (ko) 2009-04-03 2011-12-15 더 맥클린 하스피털 코퍼레이션 유도된 다능성 줄기 세포
US20120115225A1 (en) 2009-04-23 2012-05-10 Xu C W Reprogramming of somatic cells with purified proteins
EP2253700A1 (en) 2009-05-13 2010-11-24 Helmholtz-Zentrum für Infektionsforschung GmbH A method for producing test systems from donors suffering from adverse effects of medicaments and /or medical treatments, and uses of said systems
JP2013510563A (ja) 2009-11-13 2013-03-28 アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル 操作された微小胞を用いた真核細胞のリプログラミング
EP2531599A4 (en) * 2010-02-04 2014-01-08 Vivoscript Inc COMPOSITIONS AND METHODS OF RENEWING CELLS WITHOUT GENETIC MODIFICATION FOR THE TREATMENT OF NEUROLOGICAL SUFFERING
WO2012116064A1 (en) * 2011-02-22 2012-08-30 The Board Of Regents Of The University Of Texas Cardiac repair by reprogramming of cardiac fibroblasts into cardiomyocytes
WO2013033213A1 (en) 2011-08-30 2013-03-07 The J. David Gladstone Institutes Methods for generating cardiomyocytes
US20180223260A1 (en) * 2011-10-21 2018-08-09 Stemgenics, Inc. Functionalized nanoparticles for the intracellular delivery of biologically active molecules and methods for their manufacture and use

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090298111A1 (en) * 2008-05-28 2009-12-03 Wayne State University Method and composition for a protein transduction technology and its applications
CN102272142A (zh) * 2008-12-23 2011-12-07 帷幄生物技术公司 非遗传修饰性重编程细胞的组合物和方法
CN102115760A (zh) * 2009-12-30 2011-07-06 高丽大学校产学协力团 使体细胞重编程以形成诱导全能干细胞的组合物,和用其形成诱导全能干细胞的方法
WO2012022725A2 (en) * 2010-08-19 2012-02-23 F. Hoffmann-La Roche Ag Conversion of somatic cells to induced reprogrammed neural stem cells (irnscs)
WO2012151234A2 (en) * 2011-05-02 2012-11-08 Wayne State University A protein-induced pluripotent cell technology uses thereof
WO2013013105A2 (en) * 2011-07-19 2013-01-24 Vivoscript,Inc. Compositions and methods for re-programming cells without genetic modification for repairing cartilage damage

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
穿透性Sox2蛋白的质粒构建及功能鉴定;丁道芳 等;《中国药理学通报》;20121119;第28卷(第12期);第1671-1674页 *
阳离子脂质体基因载体的细胞转染研究;姜云霞 等;《安徽农业科学》;20091231;第37卷(第17期);第7862-7864页 *

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