CN106148425B - 肌醇的制备方法 - Google Patents
肌醇的制备方法 Download PDFInfo
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- CN106148425B CN106148425B CN201510184621.4A CN201510184621A CN106148425B CN 106148425 B CN106148425 B CN 106148425B CN 201510184621 A CN201510184621 A CN 201510184621A CN 106148425 B CN106148425 B CN 106148425B
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- Prior art keywords
- inositol
- phosphorylase
- preparation
- cellulose
- cellulase
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- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 title claims abstract description 106
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 title claims abstract description 105
- 229960000367 inositol Drugs 0.000 title claims abstract description 105
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 title claims abstract description 105
- 238000002360 preparation method Methods 0.000 title claims abstract description 37
- 238000006243 chemical reaction Methods 0.000 claims abstract description 102
- 229920002678 cellulose Polymers 0.000 claims abstract description 62
- 239000001913 cellulose Substances 0.000 claims abstract description 61
- 102000004190 Enzymes Human genes 0.000 claims abstract description 57
- 108090000790 Enzymes Proteins 0.000 claims abstract description 57
- 229920002472 Starch Polymers 0.000 claims abstract description 50
- 239000008107 starch Substances 0.000 claims abstract description 50
- 235000019698 starch Nutrition 0.000 claims abstract description 50
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 22
- 230000002255 enzymatic effect Effects 0.000 claims abstract description 21
- 239000000758 substrate Substances 0.000 claims abstract description 19
- 239000008103 glucose Substances 0.000 claims abstract description 18
- 108010073135 Phosphorylases Proteins 0.000 claims description 54
- 102000009097 Phosphorylases Human genes 0.000 claims description 54
- 229940088598 enzyme Drugs 0.000 claims description 54
- 101710144867 Inositol monophosphatase Proteins 0.000 claims description 49
- 102000006029 inositol monophosphatase Human genes 0.000 claims description 46
- 108030001866 Inositol-3-phosphate synthases Proteins 0.000 claims description 42
- 239000000047 product Substances 0.000 claims description 38
- 108091000115 phosphomannomutase Proteins 0.000 claims description 36
- 108010059892 Cellulase Proteins 0.000 claims description 33
- 229940106157 cellulase Drugs 0.000 claims description 33
- 102000009569 Phosphoglucomutase Human genes 0.000 claims description 30
- 229920001503 Glucan Polymers 0.000 claims description 29
- 238000006555 catalytic reaction Methods 0.000 claims description 27
- TWNIBLMWSKIRAT-VFUOTHLCSA-N levoglucosan Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@H]2CO[C@@H]1O2 TWNIBLMWSKIRAT-VFUOTHLCSA-N 0.000 claims description 26
- 229920000137 polyphosphoric acid Polymers 0.000 claims description 25
- 108010021582 Glucokinase Proteins 0.000 claims description 21
- 102000030595 Glucokinase Human genes 0.000 claims description 21
- NBIIXXVUZAFLBC-UHFFFAOYSA-N phosphoric acid Substances OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 19
- 108090000637 alpha-Amylases Proteins 0.000 claims description 17
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 claims description 16
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 claims description 16
- 229910001425 magnesium ion Inorganic materials 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 16
- 108010057899 Maltose phosphorylase Proteins 0.000 claims description 15
- PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 claims description 15
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 13
- 108010048610 cellobiose phosphorylase Proteins 0.000 claims description 13
- 108010028688 Isoamylase Proteins 0.000 claims description 12
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 12
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 11
- 229920000388 Polyphosphate Polymers 0.000 claims description 10
- 239000001205 polyphosphate Substances 0.000 claims description 10
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- 239000012467 final product Substances 0.000 claims description 9
- TVHALOSDPLTTSR-UHFFFAOYSA-H hexasodium;[oxido-[oxido(phosphonatooxy)phosphoryl]oxyphosphoryl] phosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O TVHALOSDPLTTSR-UHFFFAOYSA-H 0.000 claims description 9
- 239000005457 ice water Substances 0.000 claims description 9
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- -1 Alcohol monophosphate Chemical class 0.000 claims description 7
- 239000007853 buffer solution Substances 0.000 claims description 7
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 6
- 229920002774 Maltodextrin Polymers 0.000 claims description 6
- 239000005913 Maltodextrin Substances 0.000 claims description 6
- 229920000881 Modified starch Polymers 0.000 claims description 6
- 108091000080 Phosphotransferase Proteins 0.000 claims description 6
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- 102000020233 phosphotransferase Human genes 0.000 claims description 6
- 239000011734 sodium Substances 0.000 claims description 6
- 229910052708 sodium Inorganic materials 0.000 claims description 6
- WAEMQWOKJMHJLA-UHFFFAOYSA-N Manganese(2+) Chemical compound [Mn+2] WAEMQWOKJMHJLA-UHFFFAOYSA-N 0.000 claims description 5
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 5
- 239000007795 chemical reaction product Substances 0.000 claims description 5
- 229910052816 inorganic phosphate Inorganic materials 0.000 claims description 5
- 229910001437 manganese ion Inorganic materials 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 4
- BHLCQSSLVPEHCW-BTVCFUMJSA-N [P].O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO Chemical compound [P].O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO BHLCQSSLVPEHCW-BTVCFUMJSA-N 0.000 claims description 3
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 claims description 3
- 229920001282 polysaccharide Polymers 0.000 claims description 3
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- GUBGYTABKSRVRQ-ASMJPISFSA-N alpha-maltose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-ASMJPISFSA-N 0.000 claims description 2
- 238000009835 boiling Methods 0.000 claims description 2
- 235000021021 grapes Nutrition 0.000 claims description 2
- 238000003786 synthesis reaction Methods 0.000 claims description 2
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- 229930182478 glucoside Natural products 0.000 claims 1
- 150000008131 glucosides Chemical class 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 15
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- 238000006460 hydrolysis reaction Methods 0.000 abstract description 12
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- 239000000243 solution Substances 0.000 description 20
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 15
- 239000007995 HEPES buffer Substances 0.000 description 15
- 108090000623 proteins and genes Proteins 0.000 description 15
- 239000002253 acid Substances 0.000 description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 10
- 238000004128 high performance liquid chromatography Methods 0.000 description 9
- 238000000338 in vitro Methods 0.000 description 9
- 230000026731 phosphorylation Effects 0.000 description 9
- 238000006366 phosphorylation reaction Methods 0.000 description 9
- NDVRKEKNSBMTAX-BTVCFUMJSA-N (2r,3s,4r,5r)-2,3,4,5,6-pentahydroxyhexanal;phosphoric acid Chemical compound OP(O)(O)=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O NDVRKEKNSBMTAX-BTVCFUMJSA-N 0.000 description 8
- 235000015424 sodium Nutrition 0.000 description 8
- 229910019142 PO4 Inorganic materials 0.000 description 7
- HXXFSFRBOHSIMQ-VFUOTHLCSA-N alpha-D-glucose 1-phosphate Chemical compound OC[C@H]1O[C@H](OP(O)(O)=O)[C@H](O)[C@@H](O)[C@@H]1O HXXFSFRBOHSIMQ-VFUOTHLCSA-N 0.000 description 7
- 230000014509 gene expression Effects 0.000 description 7
- 239000010452 phosphate Substances 0.000 description 7
- 241000588724 Escherichia coli Species 0.000 description 6
- 230000008859 change Effects 0.000 description 5
- 238000010367 cloning Methods 0.000 description 5
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 108010042149 Polyphosphate-glucose phosphotransferase Proteins 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 3
- 102100035679 Inositol monophosphatase 1 Human genes 0.000 description 3
- 102100030999 Phosphoglucomutase-1 Human genes 0.000 description 3
- 241000204666 Thermotoga maritima Species 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 239000000969 carrier Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000006073 displacement reaction Methods 0.000 description 3
- 230000009483 enzymatic pathway Effects 0.000 description 3
- 239000013604 expression vector Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 235000019813 microcrystalline cellulose Nutrition 0.000 description 3
- 239000008108 microcrystalline cellulose Substances 0.000 description 3
- 229940016286 microcrystalline cellulose Drugs 0.000 description 3
- 229920001542 oligosaccharide Polymers 0.000 description 3
- 150000002482 oligosaccharides Chemical class 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 229920000945 Amylopectin Polymers 0.000 description 2
- 229920000856 Amylose Polymers 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- 108010077004 Cellodextrin phosphorylase Proteins 0.000 description 2
- 108010046163 Glycogen Phosphorylase Proteins 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 230000003625 amylolytic effect Effects 0.000 description 2
- 239000003054 catalyst Substances 0.000 description 2
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- 108010050335 D-myo-inositol-3-phosphate synthase Proteins 0.000 description 1
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 1
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- 241000233855 Orchidaceae Species 0.000 description 1
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 description 1
- 241000193448 Ruminiclostridium thermocellum Species 0.000 description 1
- 241000160715 Sulfolobus tokodaii Species 0.000 description 1
- 241000203780 Thermobifida fusca Species 0.000 description 1
- 241000205180 Thermococcus litoralis Species 0.000 description 1
- 101100344386 Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C) jgt gene Proteins 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- INAPMGSXUVUWAF-GCVPSNMTSA-N [(2r,3s,5r,6r)-2,3,4,5,6-pentahydroxycyclohexyl] dihydrogen phosphate Chemical compound OC1[C@H](O)[C@@H](O)C(OP(O)(O)=O)[C@H](O)[C@@H]1O INAPMGSXUVUWAF-GCVPSNMTSA-N 0.000 description 1
- 238000005903 acid hydrolysis reaction Methods 0.000 description 1
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- PBAYDYUZOSNJGU-UHFFFAOYSA-N chelidonic acid Natural products OC(=O)C1=CC(=O)C=C(C(O)=O)O1 PBAYDYUZOSNJGU-UHFFFAOYSA-N 0.000 description 1
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- 235000013305 food Nutrition 0.000 description 1
- 230000009229 glucose formation Effects 0.000 description 1
- 239000000413 hydrolysate Substances 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 150000004001 inositols Chemical class 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 125000003071 maltose group Chemical group 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
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- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/0102—Cellobiose phosphorylase (2.4.1.20)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01049—Cellodextrin phosphorylase (2.4.1.49)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
- C12Y301/03025—Inositol-phosphate phosphatase (3.1.3.25)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y504/00—Intramolecular transferases (5.4)
- C12Y504/02—Phosphotransferases (phosphomutases) (5.4.2)
- C12Y504/02002—Phosphoglucomutase (5.4.2.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y505/00—Intramolecular lyases (5.5)
- C12Y505/01—Intramolecular lyases (5.5.1)
- C12Y505/01004—Inositol-3-phosphate synthase (5.5.1.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01001—Phosphorylase (2.4.1.1)
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
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CN201510184621.4A CN106148425B (zh) | 2015-04-17 | 2015-04-17 | 肌醇的制备方法 |
EP16779495.7A EP3305905B1 (en) | 2015-04-17 | 2016-03-16 | Inositol preparation method |
PCT/CN2016/076526 WO2016165520A1 (zh) | 2015-04-17 | 2016-03-16 | 肌醇的制备方法 |
KR1020177032707A KR102017024B1 (ko) | 2015-04-17 | 2016-03-16 | 이노시톨의 제조방법 |
JP2017554491A JP6685322B2 (ja) | 2015-04-17 | 2016-03-16 | イノシトールの調製方法 |
US15/783,996 US10597682B2 (en) | 2015-04-17 | 2017-10-13 | Inositol preparation method |
US16/291,377 US11028414B2 (en) | 2015-04-17 | 2019-03-04 | Inositol preparation method |
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EP (1) | EP3305905B1 (zh) |
JP (1) | JP6685322B2 (zh) |
KR (1) | KR102017024B1 (zh) |
CN (1) | CN106148425B (zh) |
WO (1) | WO2016165520A1 (zh) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10263025B2 (en) | 2015-06-05 | 2019-04-16 | Sony Corporation | Solid-state imaging sensor |
WO2017059278A1 (en) | 2015-10-02 | 2017-04-06 | Bonumose Biochem Llc | Enzymatic synthesis of d-tagatose |
DE102016201498B4 (de) * | 2016-02-01 | 2017-08-17 | Norbert Kuhl | Sauerstoffdichter lebensmittelbehälter |
CN109652473A (zh) * | 2017-10-10 | 2019-04-19 | 中国科学院天津工业生物技术研究所 | 肌醇-1-磷酸的制备方法 |
CN110714042A (zh) * | 2018-07-13 | 2020-01-21 | 中国科学院天津工业生物技术研究所 | 氨基葡萄糖的酶法制备 |
CN110857443B (zh) * | 2018-08-24 | 2022-04-22 | 中国科学院天津工业生物技术研究所 | 一种纤维素完全磷酸解产肌醇的方法 |
CN113383072A (zh) * | 2018-10-29 | 2021-09-10 | 博努莫斯股份有限公司 | 酶法生产己糖 |
CN109913489B (zh) * | 2019-04-03 | 2020-09-18 | 四川博浩达生物科技有限公司 | 由食用微生物表达的多酶反应体系制备肌醇的方法 |
CN109851413A (zh) * | 2019-04-03 | 2019-06-07 | 四川博浩达生物科技有限公司 | 一种基于酶促反应液的固体有机肥料及其制备方法 |
CN112626060B (zh) * | 2019-10-09 | 2022-05-13 | 中国科学院天津工业生物技术研究所 | 一种用于生产肌醇的固定化多酶体系以及生产肌醇的方法 |
CN112795598B (zh) * | 2019-10-25 | 2022-05-10 | 中国科学院天津工业生物技术研究所 | 一种基于硅矿化微囊固定化多酶生产肌醇的方法 |
CN112778358A (zh) * | 2019-11-08 | 2021-05-11 | 中国科学院天津工业生物技术研究所 | 一种体外多酶体系制备的小分子化合物的分离提取方法 |
CN112980858B (zh) * | 2019-12-13 | 2023-09-08 | 中国科学院天津工业生物技术研究所 | 基于人工油体制备固定化多酶生产肌醇技术 |
CN112980754B (zh) * | 2019-12-13 | 2023-11-28 | 中国科学院天津工业生物技术研究所 | 一种枯草芽孢杆菌全细胞催化淀粉制备肌醇的方法 |
CN113667686B (zh) * | 2020-05-14 | 2023-08-22 | 中国科学院微生物研究所 | 一种高效利用葡萄糖合成肌醇的大肠杆菌重组菌及其构建方法与应用 |
CN111909973B (zh) * | 2020-07-03 | 2022-01-18 | 华南理工大学 | 一种生物催化合成d-(+)-葡萄糖醛酸的方法及其应用 |
CN113957101B (zh) * | 2020-07-21 | 2023-02-28 | 山东福洋生物科技股份有限公司 | 一种重组大肠杆菌发酵生产肌醇的方法 |
CN111763696B (zh) * | 2020-09-01 | 2021-02-05 | 中国科学院天津工业生物技术研究所 | 蛋白质PfuPGM作为葡萄糖磷酸变位酶在生产肌醇中的应用 |
CN117051049B (zh) * | 2023-10-11 | 2024-01-23 | 中国科学院天津工业生物技术研究所 | 一种d-手性肌醇的制备方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1118006A (zh) * | 1994-04-19 | 1996-03-06 | 东丽株式会社 | 肌醇的制备方法以及所用微生物 |
WO2013096693A1 (en) * | 2011-12-22 | 2013-06-27 | Xyleco, Inc. | Production of sugar and alcohol from biomass |
CN104271756A (zh) * | 2011-11-14 | 2015-01-07 | 旭化成化学株式会社 | 肌肉肌醇和肌肉肌醇衍生物的制造方法 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040063184A1 (en) | 2002-09-26 | 2004-04-01 | Novozymes North America, Inc. | Fermentation processes and compositions |
JP2008054506A (ja) * | 2006-08-29 | 2008-03-13 | Shinshu Univ | 二糖製造方法 |
US7807419B2 (en) | 2007-08-22 | 2010-10-05 | E. I. Du Pont De Nemours And Company | Process for concentrated biomass saccharification |
WO2013125666A1 (ja) * | 2012-02-23 | 2013-08-29 | 株式会社日本触媒 | イノシトール高生産微生物およびそれを用いたイノシトールの製造方法 |
JP2014064513A (ja) * | 2012-09-26 | 2014-04-17 | Tokyo Institute Of Technology | 2−デオキシ−scyllo−イノソースの調製法 |
CA2914504C (en) * | 2013-06-05 | 2019-04-16 | Zhiguang ZHU | Complete oxidation of sugars to electricity by using cell-free synthetic enzymatic pathways |
US9283577B2 (en) * | 2013-06-26 | 2016-03-15 | Nelson Irrigation Corporation | Sprinkler with multi-functional, side-load nozzle |
GB201311989D0 (en) | 2013-07-04 | 2013-08-21 | Univ St Andrews | Inositol biotransformation |
-
2015
- 2015-04-17 CN CN201510184621.4A patent/CN106148425B/zh active Active
-
2016
- 2016-03-16 JP JP2017554491A patent/JP6685322B2/ja active Active
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- 2016-03-16 EP EP16779495.7A patent/EP3305905B1/en active Active
-
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- 2017-10-13 US US15/783,996 patent/US10597682B2/en active Active
-
2019
- 2019-03-04 US US16/291,377 patent/US11028414B2/en active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1118006A (zh) * | 1994-04-19 | 1996-03-06 | 东丽株式会社 | 肌醇的制备方法以及所用微生物 |
CN104271756A (zh) * | 2011-11-14 | 2015-01-07 | 旭化成化学株式会社 | 肌肉肌醇和肌肉肌醇衍生物的制造方法 |
WO2013096693A1 (en) * | 2011-12-22 | 2013-06-27 | Xyleco, Inc. | Production of sugar and alcohol from biomass |
Non-Patent Citations (2)
Title |
---|
Enzymatic transformation of nonfood biomass to starch;Chun You等;《PNAS》;20130430;第110卷(第18期);全文 * |
肌醇生产工艺进展;石起增等;《中国医药工业杂志》;20050110;第36卷(第1期);全文 * |
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EP3305905A4 (en) | 2018-09-05 |
JP2018511343A (ja) | 2018-04-26 |
WO2016165520A1 (zh) | 2016-10-20 |
EP3305905A1 (en) | 2018-04-11 |
US11028414B2 (en) | 2021-06-08 |
US20180057844A1 (en) | 2018-03-01 |
JP6685322B2 (ja) | 2020-04-22 |
US10597682B2 (en) | 2020-03-24 |
US20190194696A1 (en) | 2019-06-27 |
EP3305905B1 (en) | 2019-11-20 |
CN106148425A (zh) | 2016-11-23 |
KR102017024B1 (ko) | 2019-09-02 |
KR20170135965A (ko) | 2017-12-08 |
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