CN106139172A - A kind of bactericidal medical ultrasonic couplant and preparation method thereof - Google Patents
A kind of bactericidal medical ultrasonic couplant and preparation method thereof Download PDFInfo
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- CN106139172A CN106139172A CN201610680816.2A CN201610680816A CN106139172A CN 106139172 A CN106139172 A CN 106139172A CN 201610680816 A CN201610680816 A CN 201610680816A CN 106139172 A CN106139172 A CN 106139172A
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- locust bean
- bean gum
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- bactericidal
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- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 45
- 238000002360 preparation method Methods 0.000 title claims abstract description 26
- 229920000161 Locust bean gum Polymers 0.000 claims abstract description 38
- 239000000711 locust bean gum Substances 0.000 claims abstract description 38
- 235000010420 locust bean gum Nutrition 0.000 claims abstract description 38
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 33
- FVAUCKIRQBBSSJ-UHFFFAOYSA-M sodium iodide Chemical compound [Na+].[I-] FVAUCKIRQBBSSJ-UHFFFAOYSA-M 0.000 claims abstract description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- HPNMFZURTQLUMO-UHFFFAOYSA-N diethylamine Chemical compound CCNCC HPNMFZURTQLUMO-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000002202 Polyethylene glycol Substances 0.000 claims abstract description 10
- 229920001223 polyethylene glycol Polymers 0.000 claims abstract description 10
- 235000009518 sodium iodide Nutrition 0.000 claims abstract description 10
- 239000000203 mixture Substances 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims description 13
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims description 11
- 235000003704 aspartic acid Nutrition 0.000 claims description 11
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims description 11
- 239000000706 filtrate Substances 0.000 claims description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
- OOCUOKHIVGWCTJ-UHFFFAOYSA-N chloromethyl(trimethyl)silane Chemical compound C[Si](C)(C)CCl OOCUOKHIVGWCTJ-UHFFFAOYSA-N 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 8
- 239000012141 concentrate Substances 0.000 claims description 4
- 230000008878 coupling Effects 0.000 claims description 4
- 238000010168 coupling process Methods 0.000 claims description 4
- 238000005859 coupling reaction Methods 0.000 claims description 4
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 4
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- HPWWMXONAIDFQW-UHFFFAOYSA-N 2-chloroethyl(trimethyl)silane Chemical compound C[Si](C)(C)CCCl HPWWMXONAIDFQW-UHFFFAOYSA-N 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 239000011259 mixed solution Substances 0.000 claims description 3
- CKLJMWTZIZZHCS-UWTATZPHSA-N D-aspartic acid Chemical compound OC(=O)[C@H](N)CC(O)=O CKLJMWTZIZZHCS-UWTATZPHSA-N 0.000 abstract 2
- 125000001340 2-chloroethyl group Chemical group [H]C([H])(Cl)C([H])([H])* 0.000 abstract 1
- CKLJMWTZIZZHCS-UHFFFAOYSA-N D-OH-Asp Natural products OC(=O)C(N)CC(O)=O CKLJMWTZIZZHCS-UHFFFAOYSA-N 0.000 abstract 1
- 229960005261 aspartic acid Drugs 0.000 abstract 1
- 239000007822 coupling agent Substances 0.000 description 37
- 239000000523 sample Substances 0.000 description 15
- 238000012360 testing method Methods 0.000 description 14
- 206010052428 Wound Diseases 0.000 description 11
- 208000027418 Wounds and injury Diseases 0.000 description 11
- 241000283973 Oryctolagus cuniculus Species 0.000 description 8
- 230000001954 sterilising effect Effects 0.000 description 7
- 238000004659 sterilization and disinfection Methods 0.000 description 7
- XEFQLINVKFYRCS-UHFFFAOYSA-N Triclosan Chemical compound OC1=CC(Cl)=CC=C1OC1=CC=C(Cl)C=C1Cl XEFQLINVKFYRCS-UHFFFAOYSA-N 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 208000014674 injury Diseases 0.000 description 4
- 210000004877 mucosa Anatomy 0.000 description 4
- 230000008733 trauma Effects 0.000 description 4
- 229960003500 triclosan Drugs 0.000 description 4
- 206010040880 Skin irritation Diseases 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 230000035876 healing Effects 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 231100000475 skin irritation Toxicity 0.000 description 3
- 230000036556 skin irritation Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 206010011409 Cross infection Diseases 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 206010015150 Erythema Diseases 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 206010029803 Nosocomial infection Diseases 0.000 description 2
- 206010030113 Oedema Diseases 0.000 description 2
- 206010039509 Scab Diseases 0.000 description 2
- 238000010171 animal model Methods 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 230000000249 desinfective effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 231100000321 erythema Toxicity 0.000 description 2
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 2
- 230000007794 irritation Effects 0.000 description 2
- 238000011056 performance test Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000002604 ultrasonography Methods 0.000 description 2
- 230000029663 wound healing Effects 0.000 description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 206010053567 Coagulopathies Diseases 0.000 description 1
- 101710145505 Fiber protein Proteins 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241001495449 Robinia pseudoacacia Species 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- 206010072170 Skin wound Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- WTCBONOLBHEDIL-UHFFFAOYSA-M Sodium iodate Chemical compound [Na+].[O-]I(=O)=O WTCBONOLBHEDIL-UHFFFAOYSA-M 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000035602 clotting Effects 0.000 description 1
- 239000000701 coagulant Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000035617 depilation Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
- 238000010832 independent-sample T-test Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- ALRLJVYQOIVNRH-UHFFFAOYSA-N iodic acid;potassium Chemical compound [K].OI(=O)=O ALRLJVYQOIVNRH-UHFFFAOYSA-N 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 125000001453 quaternary ammonium group Chemical class 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 231100000075 skin burn Toxicity 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229910052979 sodium sulfide Inorganic materials 0.000 description 1
- GRVFOGOEDUUMBP-UHFFFAOYSA-N sodium sulfide (anhydrous) Chemical compound [Na+].[Na+].[S-2] GRVFOGOEDUUMBP-UHFFFAOYSA-N 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000004304 visual acuity Effects 0.000 description 1
- 230000037314 wound repair Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/22—Echographic preparations; Ultrasound imaging preparations ; Optoacoustic imaging preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
- A61K31/047—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates having two or more hydroxy groups, e.g. sorbitol
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
- A61K31/197—Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid, pantothenic acid
- A61K31/198—Alpha-aminoacids, e.g. alanine, edetic acids [EDTA]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K31/736—Glucomannans or galactomannans, e.g. locust bean gum, guar gum
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/74—Synthetic polymeric materials
- A61K31/765—Polymers containing oxygen
Abstract
The invention discloses a kind of bactericidal medical ultrasonic couplant and preparation method thereof, wherein, this bactericidal medical ultrasonic couplant consists of the following composition: modified locust bean gum, Polyethylene Glycol, glycerol, L aspartic acid and the water of surplus；Described modified locust bean gum is that locust bean gum reacts obtain with 2 chloroethyl trimethyl silanes, diethylamine in the presence of sodium iodide.The bactericidal medical ultrasonic couplant that the present invention provides has strong bactericidal action and can promote that the contact surface of wound heals, and has huge market using value.
Description
Technical field
The present invention relates to a kind of bactericidal medical ultrasonic couplant and preparation method thereof.
Background technology
Commonly use ultrasonic coupling agent in medical ultrasonic diagnosis and treatment as medium, be used for strengthening ultrasonic probe and human body skin it
Between coupling and isolation ultrasonic probe and examined area skin between air, make in sound wave conductive process reduce decay,
Result is checked accurately to obtain.Therefore, the definition of ultrasonic medical image and resolving power, except relevant to equipment, also with super
The quality of acoustic couplant is closely related.In ultrasonic medical, owing to ultrasonic probe is carried out with person under inspection's body skin or tract mucosa
Contact, during continuous ultrasound diagnosis and treatment, ultrasonic probe frequently contacts different patient, there is the potential danger of medical cross infection.
Public affairs in " hospital's ultrasonic probe and commercially available couplant product " (China's Disinfection magazine, 2012,29 (4), 209-291)
Having opened Shen et al. and inspected multiple commercially available couplant product by random samples, total number of bacteria exceeding standard rate is 40%, and the detection of one of which couplant is green
Pus bacillus.The a lot of formula of existing couplant realizes the effect of ultrasonic coupling agent sterilization, sterilization only by addition antibacterial
Agent is the best with other components do match, and not only bactericidal effect is undesirable, and the even membership that adds of antibacterial affects whole ultrasonic coupling agent
Performance, such as CN101658680B discloses a kind of ultrasonic coupling agent, this ultrasonic coupling agent uses iodine, potassium iodide, iodic acid
Potassium, hydrogen peroxide cause the allergy even skin burn of skin as sterilization component, the membership that adds of strong oxidizing property antibacterial, and
Affect the stability of ultrasonic coupling agent formulation.In ultrasonic coupling agent disclosed in it such as CN103007305B, CN105327369A
All use " triclosan " (triclosan) to add to ultrasonic coupling agent as antibacterial, and triclosan has Liver and kidney poison
Property, carcinogenecity, restriction has been made in all uses to triclosan of the U.S., European Union and China.Ultrasonic probe as precise part, one
As processing method ultrasonic probe is had damage, hospital is commonly used not to disinfect.The medical of existing a large amount of use surpasses
Acoustic couplant does not typically the most possess disinfecting and sterilizing functions, causes ultrasonic probe not reach sterilisation level.Especially, when ultrasonic spy
When head contacts with the skin having wound or tract mucosa, disinfecting of ultrasonic probe itself becomes particularly important, as
What develops a kind of skin being suitable for having wound or tract mucosa and the ultrasonic coupling that the contact surface of wound can be promoted to heal
Agent is particularly important.
In view of the excellent treatment effect of medical ultrasound equipment and its be widely applied, this area needs exploitation one tool badly
There are strong bactericidal action and the ultrasonic coupling agent that the contact surface of wound can be promoted to heal.
Summary of the invention
It is an object of the invention to overcome existing ultrasonic coupling agent bactericidal property the best and be not suitable for there is wound
The defect of contact surface, it is provided that a kind of have strong bactericidal action and the antibacterial type ultrasonic coupling that the contact surface of wound can be promoted to heal
Agent and preparation method thereof.
To achieve these goals, the present invention provides a kind of bactericidal medical ultrasonic couplant, wherein, this bactericidal medical
Ultrasonic coupling agent consists of the following composition: modified locust bean gum, Polyethylene Glycol, glycerol, ASPARTIC ACID and surplus
Water；Described modified locust bean gum is that locust bean gum reacts with 2-chloroethyl trimethyl silane, diethylamine in the presence of sodium iodide
Arrive.
In the present invention, this bactericidal medical ultrasonic couplant weight percentage composition can be:
Modified locust bean gum 3～10%
Polyethylene Glycol 3～8%
Glycerol 3～12%
ASPARTIC ACID 0.3～0.7%
And the water of surplus.
In the present invention, the preparation method of described modified locust bean gum includes: under nitrogen protection, by locust bean gum, iodine
Change sodium, chloromethyl trimethyl silane, diethylamine join in reaction bulb, and 50～55 DEG C are reacted 10～15 hours, are cooled to room temperature,
Filtering, filtrate concentrates, and normal hexane is recrystallized to give modified locust bean gum.In the case of in the present invention, it is preferred to, at modified locust bean gum
In the preparation method of glue, locust bean gum, sodium iodide, chloromethyl trimethyl silane, the consumption weight ratio of diethylamine be 1:0.3～
0.4:0.4～0.5:0.2～0.3.It is further preferred that in the preparation method of modified locust bean gum, locust bean gum, iodate
Sodium, chloromethyl trimethyl silane, the consumption weight ratio of diethylamine are 1:0.3:0.4:0.2.
The present invention also provides for the preparation method of a kind of above-mentioned bactericidal medical ultrasonic couplant, and this preparation method includes:
1) Polyethylene Glycol, glycerol and water are mixed, be heated to 75～85 DEG C, be incubated 10 minutes；
2) ASPARTIC ACID, modified locust bean gum are sequentially added into step 1) heat mixed solution in, filtered while hot；
3) by step 2) filtrate that is filtrated to get is cooled to room temperature, supersound process 5～10min, and obtain bactericidal medical and surpass
Acoustic couplant.
It was found by the inventors of the present invention that by locust bean gum in the presence of sodium iodide with 2-chloroethyl trimethyl silane, diethyl
Amine carries out reaction can obtain modified locust bean gum, and modified locust bean gum is organosilicon and quaternary ammonium salt-modified product, with poly-second
Glycol, glycerol, ASPARTIC ACID and water cooperation are made colorless transparent gel shape medical ultrasonic coupling agent and can be sterilized by force
And promote the contact surface healing of wound.The antibacterial type ultrasonic coupling agent of the present invention can comply fully with YY 0299-2008 mark
Accurate.
Compared with prior art, advantages of the present invention is: the antibacterial type ultrasonic coupling agent of the present invention need not additionally add
Antibacterial, it is to avoid be individually added into the antibacterial impact on ultrasonic coupling agent stability etc.；The ultrasonic coupling agent of the present invention is to work
Do not stimulate with position；The antibacterial type ultrasonic coupling agent range of application of the present invention is wider, may be used for having the skin of wound, have wound
Mucosa etc., and simultaneously have promote wound healing effect；The ultrasonic coupling agent attenuation quotient of the present invention is little, probe and
Form good infiltration, not mummification after coating between skin, it is possible to keep viscosity, pop one's head in along the detection smooth and easy sliding in position, and in
Water solublity, easy cleaning.The antibacterial type ultrasonic coupling agent preparation technology of the present invention is simple, is particularly suitable for large-scale production.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is expanded on further.But these embodiments be only limitted to illustrate the present invention and not
It it is the further restriction to protection scope of the present invention.In the present invention, all statistics are all carried out in SPSS19.0 software, its
In, measurement data independent samples t-test, ranked data rank test.
Preparation example 1
Under nitrogen protection, 100g locust bean gum, 30g sodium iodide, 40g chloromethyl trimethyl silane, 25g diethylamine are added
Entering in reaction bulb, 55 DEG C are reacted 10 hours, are cooled to room temperature, filter, and filtrate concentrates, and it is modified that normal hexane is recrystallized to give 98g
Locust bean gum G1.
Preparation example 2
Under nitrogen protection, 100g locust bean gum, 10g sodium iodide, 40g chloromethyl trimethyl silane, 40g diethylamine are added
Entering in reaction bulb, 70 DEG C are reacted 10 hours, are cooled to room temperature, filter, and filtrate concentrates, and it is modified that normal hexane is recrystallized to give 82g
Locust bean gum G2.
Embodiment 1
A kind of bactericidal medical ultrasonic couplant, its preparation method is as follows:
1) 5g Polyethylene Glycol, 12g glycerol and 75.7g water are mixed, be heated to 80 DEG C, be incubated 10 minutes；
2) 0.3g ASPARTIC ACID, 7g modification locust bean gum (preparation example 1G1) are sequentially added into step 1) heat mixed
Close in solution, filtered while hot；
3) by step 2) filtrate that is filtrated to get is cooled to room temperature, supersound process 10min, and obtain colorless transparent gel shape and kill
Bacterial type medical ultrasonic coupling agent S1.
Embodiment 2
A kind of bactericidal medical ultrasonic couplant, its preparation method is as follows:
1) 8g Polyethylene Glycol, 3g glycerol and 85.3g water are mixed, be heated to 80 DEG C, be incubated 10 minutes；
2) 0.7g ASPARTIC ACID, 3g modification locust bean gum (preparation example 1G1) are sequentially added into step 1) heat mixed
Close in solution, filtered while hot；
3) by step 2) filtrate that is filtrated to get is cooled to room temperature, supersound process 10min, and obtain colorless transparent gel shape and kill
Bacterial type medical ultrasonic coupling agent S2.
Embodiment 3
A kind of bactericidal medical ultrasonic couplant, its preparation method is as follows:
1) 3g Polyethylene Glycol, 7g glycerol and 79.5g water are mixed, be heated to 75 DEG C, be incubated 10 minutes；
2) 0.5g ASPARTIC ACID, 10g modification locust bean gum (preparation example 1G1) are sequentially added into step 1) heat
In mixed solution, filtered while hot；
3) by step 2) filtrate that is filtrated to get is cooled to room temperature, supersound process 5min, and obtain colorless transparent gel shape and kill
Bacterial type medical ultrasonic coupling agent S3.
Embodiment 4
Bactericidal medical ultrasonic couplant as described in Example 1, except that, use the G2 of identical weight to substitute
G1, obtains colorless transparent gel shape medical ultrasonic coupling agent S4.
Comparative example 1
Ultrasonic coupling agent as described in Example 1, except that, in preparation method, use the Robinia pseudoacacia L. of identical weight
Locust beam gum substitutes modified locust bean gum, obtains ultrasonic coupling agent DS1.
Comparative example 2
Ultrasonic coupling agent as described in Example 1, except that, in preparation method, do not use ASPARTIC ACID,
To ultrasonic coupling agent DS2.
Test case
One, ultrasonic coupling agent performance test
Ultrasonic coupling agent S1-S4 and DS1, DS2 have been carried out performance test, and concrete outcome is as shown in table 1.
Table 1
The velocity of sound is measured under 35 DEG C and 4.0MHz according to the method for GB/T15261, and sample length is 6cm；
Viscosity is rotary viscous according to " Pharmacopoeia of People's Republic of China two " version annex VI G " viscosimetry " in 2010
Degree meter method is measured at 25 DEG C；
PH value is measured according to " Pharmacopoeia of People's Republic of China two " 2010 version annex VI H " pH value algoscopy "；
Acoustic attenuation measures by double sample methods under 35 DEG C and 4.0MHz according to the method in GB/T15261, and with sound in water
Decay is modified, and the difference of two sample lengths is more than 5cm.
Two, ultrasonic coupling agent irritation test
1, Skin Irritation Test
Test method: (2.0 ± 0.2Kg, by Beijing Vital River Experimental Animals Technology Co., Ltd. to take 48 healthy rabbits
There is provided), male and female half and half, at random rabbit is divided into 14 groups of (often group male and female half and half, 7 groups of intact skin groups, 7 groups of wounds according to sex
Skin group), by hair cutting (the unilateral shaving area about 40cm of rabbit spinal column both sides2), and uniform application 10% sodium sulfide is de-
Except residue hair stubble, observe the depilation equal not damaged of skin, carry out modeling.To trauma skin group, with sterilizing No. 16 syringe needles in unhairing
Mark about 2cm on skin #4, not hinder corium, to have slight oozing of blood to be advisable.Test uses own control, and left side does not process and does
On the right side of rabbit in blank, 6 groups of intact skin groups and 6 groups of trauma skin groups unhairing region smear respectively S1, S2, S3, S4,
DS1, DS2, use distilled water to clean after 24 hours, after cleaning, position skin is smeared in 1h, 24h, 48h, 72h perusal
Skin, and record the situation that erythema and edema occur.
Skin wound repair standards of grading such as table 2.
Table 2
Skin irritation intensity evaluation according to score value is: 0～0.5 is nonirritant；0.5～2.0 is minimal irritation；2.0
～6.0 be moderate zest；6.0～8.0 is strong and stimulating.
The concrete result of the test of skin irritation is as shown in table 3.
Table 3
The ultrasonic coupling agent (the particularly ultrasonic coupling agent in embodiment 1-3) of the present invention is the most right as can be seen from the above table
Intact skin is still to trauma skin all nonirritants.It addition, according to above-mentioned erythema and edema situation, find that the present invention's is ultrasonic
Couplant to intact skin and trauma skin all without sensitization.
Three, ultrasonic coupling agent bactericidal effect test
S1-S4 and DS1, DS2 being applied to ultrasonic probe, detects after 2 minutes, testing result is as shown in table 4.
Table 4
Bactericidal property is carried out according to regulation C.3 in GB/T15979-2002 appendix C.
As can be seen from the above table, the ultrasonic coupling agent of the present invention all has superpower sterilization energy for various common bacterias
Power, thus ensured that ultrasonic probe will not produce nosocomial infection to patient during frequently using.
Four, the coagulant blood of ultrasonic coupling agent and rush skin healing test
1, each to S1-S4 and DS1, DS2 1g is added separately in test tube, takes rabbit arteria auricularis blood, rapid injecting tube,
Every test tube 1ml, shakes up after adding, timing immediately.1 blank test tube is additionally set, is not added with any ultrasonic coupling agent.Often group is flat
Four tests of row, statistical result such as table 5.
Table 5
| Start clotting time/s | Complete setting time/the s of blood | |
| S1 | 362±27 | 471±29 |
| S2 | 371±32 | 474±40 |
| S3 | 365±36 | 467±51 |
| S4 | 384±42 | 511±43 |
| DS1 | 402±33 | 547±40 |
| DS2 | 422±27 | 564±43 |
| Blank | 451±37 | 577±45 |
2,28 healthy rabbits (1.8 ± 0.5Kg is provided) are taken by Beijing Vital River Experimental Animals Technology Co., Ltd.,
Male and female half and half, are divided into 7 groups (often group male and female half and half) at random, by ether inhalation anesthesia, slough by portion's hair according to sex by rabbit, raw
With 2% iodine tincture, 75% ethanol successively sterilization after reason saline cleaning, drying, excise the circular full thickness skin of a diameter of 0.5cm.Its
In 6 groups of respectively external application S1-S4 and DS1, DS2, remain one group and do not process, as blank group.Conventional raising, observes wound
Mouth healing state, and record, concrete outcome is as shown in table 6.
Table 6
| Smear | Rabbit quantity/only | Average scab forming time/h | Average decrustation time/h |
| S1 | 4 | 23 | 156 |
| S2 | 4 | 26 | 161 |
| S3 | 4 | 25 | 162 |
| S4 | 4 | 32 | 190 |
| DS1 | 4 | 40 | 210 |
| DS2 | 4 | 45 | 224 |
| Blank | 4 | 50 | 265 |
Scab forming time is the time occurring fiber protein yarn bottom test tube.
From table 5, table 6 it can be seen that the present invention bactericidal medical ultrasonic couplant also have promotion blood coagulation and
The effect of wound healing.
To sum up, the invention provides a kind of bactericidal medical ultrasonic couplant, this bactericidal medical ultrasonic couplant has
Strong bactericidal action and the ultrasonic coupling agent that the contact surface of wound can be promoted to heal.
Claims (6)
1. a bactericidal medical ultrasonic couplant, it is characterised in that this bactericidal medical ultrasonic couplant is by following component group
Become: modified locust bean gum, Polyethylene Glycol, glycerol, ASPARTIC ACID and the water of surplus；Described modified locust bean gum is thorn
Locust bean gum reacts obtain with 2-chloroethyl trimethyl silane, diethylamine in the presence of sodium iodide.
Bactericidal medical ultrasonic couplant the most according to claim 1, it is characterised in that this bactericidal medical ultrasonic couples
Agent weight percentage composition is:
Modified locust bean gum 3～10%
Polyethylene Glycol 3～8%
Glycerol 3～12%
ASPARTIC ACID 0.3～0.7%
And the water of surplus.
Bactericidal medical ultrasonic couplant the most according to claim 1 and 2, it is characterised in that described modified locust bean gum
Preparation method include: under nitrogen protection, locust bean gum, sodium iodide, chloromethyl trimethyl silane, diethylamine are joined instead
Answering in bottle, in THF, 50～55 DEG C are reacted 10～15 hours, are cooled to room temperature, filter, and filtrate concentrates, and normal hexane is recrystallized to give
Modified locust bean gum.
4. according to the bactericidal medical ultrasonic couplant described in claim 1-3, it is characterised in that in the system of modified locust bean gum
In Preparation Method, locust bean gum, sodium iodide, chloromethyl trimethyl silane, the consumption weight ratio of diethylamine are 1:0.3～0.4:0.4
～0.5:0.2～0.3.
Bactericidal medical ultrasonic couplant the most according to claim 4, it is characterised in that in the preparation of modified locust bean gum
In method, locust bean gum, sodium iodide, chloromethyl trimethyl silane, the consumption weight ratio of diethylamine are 1:0.3:0.4:0.25.
6. the preparation method of bactericidal medical ultrasonic couplant described in any one in claim 1-5, it is characterised in that should
Preparation method includes:
1) Polyethylene Glycol, glycerol and water are mixed, be heated to 75～85 DEG C, be incubated 10 minutes；
2) ASPARTIC ACID, modified locust bean gum are sequentially added into step 1) heat mixed solution in, filtered while hot；
3) by step 2) filtrate that is filtrated to get is cooled to room temperature, supersound process 5～10min, obtains bactericidal medical ultrasonic coupling
Mixture.
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| CN106729925A (en) * | 2017-03-29 | 2017-05-31 | 邱发龙 | The preparation method and gel spray of a kind of gel spray repaired for wound |
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| CN102512696A (en) * | 2011-12-31 | 2012-06-27 | 戴新春 | Medical ultrasonic couplant and preparation method thereof |
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Application publication date: 20161123 |