CN106137814A - A kind of natural antiseptic agent with broad-spectrum antibacterial action - Google Patents
A kind of natural antiseptic agent with broad-spectrum antibacterial action Download PDFInfo
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- CN106137814A CN106137814A CN201510164382.6A CN201510164382A CN106137814A CN 106137814 A CN106137814 A CN 106137814A CN 201510164382 A CN201510164382 A CN 201510164382A CN 106137814 A CN106137814 A CN 106137814A
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- 239000004599 antimicrobial Substances 0.000 title claims abstract description 23
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 24
- 241000894006 Bacteria Species 0.000 claims description 17
- 230000000694 effects Effects 0.000 claims description 17
- 239000000284 extract Substances 0.000 claims description 17
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 14
- 238000005325 percolation Methods 0.000 claims description 12
- 230000002829 reductive effect Effects 0.000 claims description 12
- 239000012141 concentrate Substances 0.000 claims description 11
- 239000000706 filtrate Substances 0.000 claims description 11
- 239000002904 solvent Substances 0.000 claims description 11
- 239000006228 supernatant Substances 0.000 claims description 11
- 239000003755 preservative agent Substances 0.000 claims description 10
- 230000002335 preservative effect Effects 0.000 claims description 10
- 241000228245 Aspergillus niger Species 0.000 claims description 8
- 241000588724 Escherichia coli Species 0.000 claims description 8
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims description 7
- 241000191967 Staphylococcus aureus Species 0.000 claims description 6
- 241000222122 Candida albicans Species 0.000 claims description 5
- 229940095731 candida albicans Drugs 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 239000002994 raw material Substances 0.000 claims 1
- 239000003814 drug Substances 0.000 abstract description 10
- 239000007788 liquid Substances 0.000 abstract description 7
- 238000002360 preparation method Methods 0.000 abstract description 6
- 238000001228 spectrum Methods 0.000 abstract description 5
- 230000000845 anti-microbial effect Effects 0.000 abstract description 4
- 239000012535 impurity Substances 0.000 abstract description 4
- 238000000605 extraction Methods 0.000 abstract description 3
- 239000004615 ingredient Substances 0.000 abstract description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
- 238000012360 testing method Methods 0.000 description 11
- 239000002609 medium Substances 0.000 description 9
- 230000000052 comparative effect Effects 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 6
- 239000007787 solid Substances 0.000 description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 230000001629 suppression Effects 0.000 description 5
- 239000001888 Peptone Substances 0.000 description 4
- 108010080698 Peptones Proteins 0.000 description 4
- 229940041514 candida albicans extract Drugs 0.000 description 4
- 239000002537 cosmetic Substances 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 235000019319 peptone Nutrition 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 239000012138 yeast extract Substances 0.000 description 4
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 238000004500 asepsis Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000002421 anti-septic effect Effects 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000000686 essence Substances 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- WPYMKLBDIGXBTP-UHFFFAOYSA-N Benzoic acid Natural products OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 1
- 239000005711 Benzoic acid Substances 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 239000009636 Huang Qi Substances 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 235000010233 benzoic acid Nutrition 0.000 description 1
- -1 benzoic acid ester Chemical class 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 229940126678 chinese medicines Drugs 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 210000000918 epididymis Anatomy 0.000 description 1
- 201000010063 epididymitis Diseases 0.000 description 1
- 230000032050 esterification Effects 0.000 description 1
- 238000005886 esterification reaction Methods 0.000 description 1
- 230000001408 fungistatic effect Effects 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 description 1
- 235000014593 oils and fats Nutrition 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000011265 semifinished product Substances 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 230000035943 smell Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 210000001550 testis Anatomy 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 210000004291 uterus Anatomy 0.000 description 1
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- Cosmetics (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses a kind of natural antiseptic agent with broad-spectrum antibacterial action, comprise Fructus Litseae, Herba Pogostemonis and Herba Cymbopogonis Citrari, wherein, the weight proportion of described Fructus Litseae is 10～20 parts, the weight proportion 10 of described Herba Pogostemonis～20 parts, the weight proportion 10 of described Herba Cymbopogonis Citrari～20 parts.Using technical scheme, the Chinese medicine extraction liquid color that conventional preparation techniques can be overcome to prepare is deep, and effective ingredient retains few, the problems such as impurity is many, narrow antimicrobial spectrum.
Description
Technical field
The present invention relates to a kind of natural antiseptic agent, and its application, and the preparation method of this preservative.
Background technology
At present people use more be synthetic preservative, but find through long-term research, some synthesis
Preservative lures carcinous, teratogenecity, and human body can be caused injury greatly by life-time service.Such as, to hydroxyl
Yl benzoic acid ester can make tumor cell proliferation, increases animal uterus weight, affects the sperm of testis and epididymis
Quantity and form etc..Further, life-time service synthetic preservative, antibacterial can produce certain drug resistance.
Therefore, natural antiseptic agent replaces synthetic preservative to have become as a study hotspot.
And currently used natural antiseptic agent mostly is semifinished product, its color is relatively deep, and abnormal smells from the patient is denseer, antimicrobial spectrum
The narrowest.Meanwhile, some Chinese medicine is used in combination rear antibacterial effect not as single medicinal material.As the Radix Astragali and Radix Scutellariae mix
After closing proportioning, escherichia coli, bacillus pyocyaneus and Salmonella bacteriostasis are weaker than and are used alone.
Natural antiseptic agent is used to replace synthetic preservative to become an important development in cosmetics
Direction, has the biggest market space, how to prepare and meets cosmetics interpolation requirement i.e. high concentration face again
The extracting solution that color is shallow, the preservative for broad-spectrum antiseptic is current urgent problem simultaneously.
Summary of the invention
In view of this, the invention provides a kind of natural antiseptic agent with broad-spectrum antibacterial action, comprise mountain
Grey son, Herba Pogostemonis and Herba Cymbopogonis Citrari, wherein, the weight proportion of described Fructus Litseae is 10～20 parts, the described wide leaves of pulse plants
Fragrant weight proportion 10～20 parts, the weight proportion 10 of described Herba Cymbopogonis Citrari～20 parts.
Present invention also offers the effect in antibacterial of a kind of above-mentioned natural antiseptic agent, wherein, described bacterium
Kind is staphylococcus aureus, escherichia coli, bacillus pyocyaneus, Candida albicans and/or aspergillus niger.
Present invention also offers the extracting method of a kind of above-mentioned natural antiseptic agent, it is characterised in that include as
Lower feature:
(1) by 10～20 parts of Fructus Litseae, 10～20 parts of Herba Pogostemonis, 10～20 portions of Herba Cymbopogonis Citrari add 20～30 times
The alcohol percolation method of 80wt%～95wt% extracts；
(2) seepage pressure effects 24 hours, filter, take filtrate；
(3) concentrate under reduced pressure at low temperature removes solvent, is replaced as GTCC；
(4) it is aged 12 hours at-10 DEG C, filters after layering, take supernatant, obtain described natural
Preservative extract.
Use technical scheme, Chinese medicine extraction liquid color prepared by conventional preparation techniques can be overcome
Deeply, effective ingredient retains few, the problems such as impurity is many, narrow antimicrobial spectrum.Should by compatibility rational between Chinese medicine
With, make extract have the effect of broad-spectrum antiseptic.And the damage of volatile material is reduced by the method for percolation
Lose, go out partial impurities by ageing, thus preferably retain small-molecule substance, make meeting cosmetics
With requiring and aesthetically having significant contribution.
Detailed description of the invention
Technical scheme is further illustrated below by detailed description of the invention.May be appreciated
It is that specific embodiment described herein is used only for explaining the present invention, rather than limitation of the invention.
Embodiment 1
(1) take 3g Fructus Litseae, 2g part Herba Pogostemonis, 4g part Herba Cymbopogonis Citrari, add the ethanol of 270g 80wt%
Percolation extracts；
(2) seepage pressure effects 24 hours, filter, take filtrate；
(3) concentrate under reduced pressure at low temperature removes solvent, be replaced as GTCC (GTCC for by caprylic/capric and
The high-purity oils and fats of glycerine esterification)；
(4)-10 DEG C are aged 12 hours, filter, take supernatant, obtain natural antiseptic agent and carry after layering
Take thing.
Embodiment 2
(1) take 2g Fructus Litseae, 4g Herba Pogostemonis, 3g Herba Cymbopogonis Citrari, add the ethanol percolation of 225g 90wt%
Method is extracted；
(2) seepage pressure effects 24 hours, filter, take filtrate；
(3) concentrate under reduced pressure at low temperature removes solvent, is replaced as GTCC；
(4)-10 DEG C are aged 12 hours, filter, take supernatant after layering, obtain natural antiseptic agent and extract
Thing.
Embodiment 3
(1) take 4g Fructus Litseae, 3g Herba Pogostemonis, 2g Herba Cymbopogonis Citrari, add the ethanol percolation of 180g 95wt%
Method is extracted；
(2) seepage pressure effects 24 hours, filter, take filtrate；
(3) concentrate under reduced pressure at low temperature removes solvent, is replaced as GTCC；
(4)-10 DEG C are aged 12 hours, filter, take supernatant after layering, obtain natural antiseptic agent and extract
Thing.
Comparative example 1
(1) alcohol percolation method adding 180g 95wt% in 9g Fructus Litseae extracts；
(2) seepage pressure effects 24 hours, filter, take filtrate；
(3) concentrate under reduced pressure at low temperature removes solvent, is replaced as GTCC；
(4)-10 DEG C are aged 12 hours, filter, take supernatant after layering, obtain natural antiseptic agent and extract
Thing.
Comparative example 2
(1) alcohol percolation method adding 180g 95wt% in 9g Herba Pogostemonis extracts；
(2) seepage pressure effects 24 hours, filter, take filtrate；
(3) concentrate under reduced pressure at low temperature removes solvent, is replaced as GTCC；
(4)-10 DEG C are aged 12 hours, filter, take supernatant, obtain natural antiseptic agent extract after layering.
Comparative example 3
(1) alcohol percolation method adding 180g 95wt% in 9g Herba Cymbopogonis Citrari extracts；
(2) seepage pressure effects 24 hours, filter, take filtrate；
(3) concentrate under reduced pressure at low temperature removes solvent, is replaced as GTCC；
(4)-10 DEG C are aged 12 hours, filter, take supernatant, obtain natural antiseptic agent extract after layering.
Comparative example 4
(1) taking 5g Fructus Litseae, 3g Herba Pogostemonis, 1g Herba Cymbopogonis Citrari, the alcohol percolation method adding 180g 95wt% carries
Take；
(2) seepage pressure effects 24 hours, filter, take filtrate；
(3) concentrate under reduced pressure at low temperature removes solvent, is replaced as GTCC；
(4)-10 DEG C are aged 12 hours, filter, take supernatant, obtain natural antiseptic agent extract after layering.
Comparative example 5
(1) taking 1g Fructus Litseae, 5g Herba Pogostemonis, 3g Herba Cymbopogonis Citrari, the alcohol percolation method adding 180g 95wt% carries
Take；
(2) seepage pressure effects 24 hours, filter, take filtrate；
(3) concentrate under reduced pressure at low temperature removes solvent, is replaced as GTCC；
(4)-10 DEG C are aged 12 hours, filter, take supernatant, obtain natural antiseptic agent extract after layering.
Comparative example 6
(1) taking 3g Fructus Litseae, 1g Herba Pogostemonis, 5g Herba Cymbopogonis Citrari, the alcohol percolation method adding 180g 95wt% carries
Take；
(2) seepage pressure effects 24 hours, filter, take filtrate；
(3) concentrate under reduced pressure at low temperature removes solvent, is replaced as GTCC；
(4)-10 DEG C are aged 12 hours, filter, take supernatant, obtain natural antiseptic agent extract after layering.
Bacteriostatic test step:
(1) test strain
Staphylococcus aureus (ATCC 8739), escherichia coli (ATCC 6538), bacillus pyocyaneus (ATCC
9027), candida albicans (ATCC 10231), Aspergillus niger (ATCC 16404).
(2) culture medium
TSB solid medium: peptone 10g/L, NaCl 10g/L, K2HPO42.5g/L, yeast
Extract 3g/L, agar 15g/L, distilled water dissolves, and regulation pH is 7.2 ± 0.2,115 DEG C of high pressure
Sterilizing 20min；TSB fluid medium: peptone 10g/L, NaCl 10g/L, K2HPO42.5g/L,
Yeast extract 3g/L, distilled water dissolves, and regulation pH is 7.2 ± 0.2,115 DEG C of autoclaving 20min；
SDB solid medium: peptone 10g/L, glucose 20g/L, K2HPO43g/L, yeast
Extract 5g/L, agar 15g/L, distilled water dissolves, 115 DEG C of autoclaving 20min；SDB liquid
Body culture medium: peptone 10g/L, glucose 20g/L, K2HPO43g/L, yeast extract 5g/L,
Distilled water dissolves, 115 DEG C of autoclaving 20min.
(3) bacteria suspension preparation and inoculation
Taking the staphylococcus aureus of glycerol stocks, escherichia coli, bacillus pyocyaneus 100 μ L coats TSB
On solid medium, cultivate 2d for 37 DEG C, resuspended with normal saline, obtain bacteria suspension, and train with flat board
The method of supporting counting, is adjusted to 10 by test bacteria concentration5～106CFU/mL。
Candida albicans and Aspergillus niger 100 μ L coat on SDB solid medium, 30 DEG C of trainings
Support 2d, resuspended with normal saline, obtain bacteria suspension, and count by plating method, bacterium will be tested dense
Degree is adjusted to 105～106。
(4) mensuration of sample bacteriostasis
Cylinder plate method
In culture dish, pour TSB or SDB solid medium into, to be solidified after, in this culture medium all
Even coating test bacterium 100 μ L.In culture medium equidistant put 2 aseptic Oxford cups [internal diameter (6.0 ±
0.1) mm, external diameter (8.0 ± 0.1) mm, high (10.0 ± 0.1) mm, in cup, it is injected separately into 200 μ L treats
Test sample product, with normal saline or containing the normal saline of a certain amount of DMSO as comparison.37 DEG C or 30 DEG C
After 2d cultivated by calorstat, measure the antibacterial circle diameter of various medicines with ruler, take its meansigma methods.
Microdilution plate method
In micropore, add 100 μ L testing sample (initial concentration), add containing test bacteria concentration 1.0
×105CFU/mL is 2 × TSB or 2 × SDB fluid medium 100 μ L, mixing, i.e. treats test sample
The test concentrations of product is the 50wt% of initial concentration.After 2d cultivated by 30 DEG C or 37 DEG C of calorstats, observe
There is asepsis growth.The premise that result judges is that growth control is good, and blank asepsis growth is clear.
(5) mensuration of minimum inhibitory concentration (MIC value)
Minimum suppression staphylococcus aureus concentration (MIC), minimum suppression e. coli concentration (MIC),
Minimum suppression bacillus pyocyaneus concentration (MIC), the mensuration of minimum suppression candida albicans concentration (MIC)
Method is as follows:
(1) prepared by medicinal liquid
Testing sample normal saline dilution is become experimental concentration.
(2) bacterium solution preparation 2 × TSB or 2 × SDB fluid medium dilutes bacteria suspension so that it is
Whole bacteria concentration is l05CFU/mL。
(3) cultivate and result interpretation adds bacterium solution 100 μ L and medicinal liquid 100 μ L, simultaneously in micropore
If the negative control being not added with bacterium and the normal growth comparison being not added with medicinal liquid, every kind of medicine do 3 parallel, make even
Average.Put 37 DEG C or 30 DEG C of wet boxes are hatched, observed result after 48h, use direct method to read data.
The premise that result judges is that growth control is good, and blank asepsis growth is clear, and other hole is dense with medicine
Degree gradient raises and the growth of bacterium is suppressed.
Minimum suppression aspergillus niger concentration (MIC) measures
Use cylinder plate method, culture dish pour into SDB solid medium, to be solidified after, in this culture medium
Add Aspergillus niger (105CFU/ml) 100 μ L, even spread.Equidistant in culture medium put 3
Individual aseptic Oxford cup [internal diameter (6.0 ± 0.1) mm, external diameter (8.0 ± 0.1) mm, high (10.0 ± 0.1) mm,
The sample (with normal saline dilution) of 200 μ L variable concentrations it is injected separately in Bei.Set simultaneously and be not added with surveying
The normal growth comparison of test agent and saline control.The premise that result judges is growth control and physiology
Saline control well-grown, other raises with drug concentration gradient and the growth of bacterium is suppressed.
The present invention is compared with single medicinal material, and the antibacterial result of product is as shown in table 1:
Table 1
The present invention is compared with different proportion Chinese medicine, and the antibacterial result of product is as shown in table 2:
Table 2
As shown in Table 1, Fructus Litseae is more weak to the antibacterial effect of escherichia coli and Aspergillus niger；Herba Pogostemonis pair
Staphylococcus aureus and Aspergillus niger are without antibacterial effect；Herba Cymbopogonis Citrari is more weak to bacillus pyocyaneus antibacterial effect, right
Escherichia coli are without antibacterial effect.As shown in Table 2, the formula of comparative example, the antibacterial effect of product are used
Fruit is much smaller than the effect of the embodiment of the present invention.The prescription bacteriostasis efficacy of the present invention is notable, has the anti-of wide spectrum
Bacterium effect, its fungistatic effect is better than the effect of single medicinal material, overcomes the single medicinal material limitation to some bacterial strain
Property.Meanwhile, the ratio of three kinds of Chinese medicines of this invention can suppress the growth of antibacterial to greatest extent.It is suitable for
Using as natural antiseptic agent, process program maintains bacteriostasis efficacy, and significantly reduces the color of product,
Product is stable, is suitable for addition in cosmetics use as preservative.
In sum, use the technical scheme of the embodiment of the present invention, conventional preparation techniques can be overcome to prepare
Chinese medicine extraction liquid color is deep, and effective ingredient retains few, the problems such as impurity is many, narrow antimicrobial spectrum.
The present invention also can have other various embodiments, without departing substantially from present invention spirit and the situation of essence thereof
Under, those of ordinary skill in the art work as can make various corresponding change and deformation according to the present invention, but
These change accordingly and deform the protection domain that all should belong to appended claims of the invention.
These are only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Within god and principle, any modification, equivalent substitution and improvement etc. made, should be included in the present invention's
Within protection domain.
Claims (3)
1. a natural antiseptic agent with broad-spectrum antibacterial action, it is characterised in that described natural anticorrosion
Agent raw material comprises Fructus Litseae, Herba Pogostemonis and Herba Cymbopogonis Citrari, and wherein, the weight proportion of described Fructus Litseae is 10～20
Part, the weight proportion 10 of described Herba Pogostemonis～20 parts, the weight proportion 10 of described Herba Cymbopogonis Citrari～20 parts.
2. a natural antiseptic agent as claimed in claim 1 effect in antibacterial, wherein, described
The kind of bacterium is staphylococcus aureus, escherichia coli, bacillus pyocyaneus, Candida albicans and/or aspergillus niger.
3. the extracting method of a natural antiseptic agent as claimed in claim 1, it is characterised in that bag
Include following feature:
(1) by 10～20 parts of Fructus Litseae, 10～20 parts of Herba Pogostemonis, 10～20 portions of Herba Cymbopogonis Citrari add 20～30 times
The alcohol percolation method of 80wt%～95wt% extracts；
(2) seepage pressure effects 24 hours, filter, take filtrate；
(3) concentrate under reduced pressure at low temperature removes solvent, is replaced as GTCC；
(4) it is aged 12 hours at-10 DEG C, filters after layering, take supernatant, obtain described natural
Preservative extract.
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| CN201510164382.6A CN106137814B (en) | 2015-04-08 | 2015-04-08 | A kind of natural antiseptic agent with broad-spectrum antibacterial action |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107087743A (en) * | 2017-07-12 | 2017-08-25 | 太仓市荣德生物技术研究所 | A kind of antibacterial antioxidation environmental protection bactericide |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101385474A (en) * | 2007-12-27 | 2009-03-18 | 广东省中药研究所 | Plant source natural bactericidal agent |
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2015
- 2015-04-08 CN CN201510164382.6A patent/CN106137814B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101385474A (en) * | 2007-12-27 | 2009-03-18 | 广东省中药研究所 | Plant source natural bactericidal agent |
Non-Patent Citations (2)
| Title |
|---|
| 张艳等: "中草药提取物在果蔬保鲜中的研究进展", 《中国食品添加剂》 * |
| 王宏年等: "7种植物精油对番茄灰霉病的抑制效果", 《植物保护》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107087743A (en) * | 2017-07-12 | 2017-08-25 | 太仓市荣德生物技术研究所 | A kind of antibacterial antioxidation environmental protection bactericide |
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