CN106137453A - A kind of Real-time Feedback multi-mode oxygen control system - Google Patents
A kind of Real-time Feedback multi-mode oxygen control system Download PDFInfo
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- CN106137453A CN106137453A CN201610752630.3A CN201610752630A CN106137453A CN 106137453 A CN106137453 A CN 106137453A CN 201610752630 A CN201610752630 A CN 201610752630A CN 106137453 A CN106137453 A CN 106137453A
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Abstract
The present invention relates to a kind of Real-time Feedback multi-mode oxygen control system, including compressed oxygen cylinder, compressed nitrogen gas cylinder, compressed air bottle, animal nacelle and single-chip computer control system, the outlet of described compressed oxygen cylinder provides gas after being pooled to air inlet pipe by nitrogen tube and compressed air bottle by air hose by the outlet of oxygen hose, compressed nitrogen gas cylinder in animal capsule body；Being respectively equipped with the first gas control equipment, the second gas control equipment and the 3rd gas control equipment on described oxygen hose, nitrogen tube and air hose, described single-chip computer control system can make the environment in animal capsule body become intermittent hypoxia pattern, Constant hypoxia pattern, normal oxygen pattern or continue hyperoxia pattern by regulation gas control equipment.The invention has the beneficial effects as follows: using blood oxygen saturation, oxygen concentration and nacelle internal gas pressure as feedback information, by regulation gas supply flow and pattern, in making nacelle, gas is uniformly distributed rapidly and reaches required for oxygen condition, simulates different oxygen environments.
Description
Technical field
The present invention relates to medical experiment field, be specifically related to a kind of Real-time Feedback multi-mode oxygen control system.
Background technology
Obstruction sleep apnea-hypopnea syndrome (OSAHS) with patient sleeps happens over and over again epithelium healing completely and
(or) incomplete obstruction causes asphyxia or low ventilation to be characterized, the Pathophysiology link of its key is chronic intermittent hypoxia
(chronic intermittent hypoxia, CIH).Because the infringement of the systems such as cardiovascular and cerebrovascular vessel is gradually closed by it by people
One of note, and the hot issue becoming Recent study.Set up preferable OSAHS animal model and there is great difficulty, therefore real
Most intermittent hypoxia animal model applying sleep apnea pattern in the research of border, simulation cycle hypoxia-reoxygenation process,
To study the Multisystem damage of OSAHS.The mode predominantly interval realizing chronic intermittent hypoxia (CIH) sucks low-oxygen gas mixture body
With interval ventilation blocked method.Wherein the former is the most most widely used CIH model.The principle of above-mentioned model is by various methods
Realize SaO in animal body2Periodically reduce and recover, thus simulating the chronic intermittent hypoxia process in OSAHS patient body.According to
Offering oxygen way is different, and the experimental provision setting up chronic intermittent hypoxia animal experimental model at present has dissimilar: 1. move back and forth
Formula hypoxia cabin, is that hypoxia cabin moves back and forth between animal capsule, makes animal capsule interval enter low-oxygen environment, can substantially simulate repeatedly
The pathophysiological features of hypoxia/reoxygenation, but high to equipment requirements, operation complexity, and can not get rid of and move repeatedly animal to experiment
The impact produced；2. hypobaric hypoxia cabin, the height above sea level different by changing the elevation rate simulation of oxygen cabin, produce different pressure
And oxygen concentration, thus reaching the effect of hypoxia-reoxygenation, the requirement to equipment is higher, it is impossible to accurately reflect the feelings of anoxia under normal pressure
Condition；The lowest O2High CO2Case, uses singlechip technology and feedback principle, is automatically adjusted O in oxygen cabin2、CO2Concentration, set up hypoxia and
CO2Retention model, it is impossible to get rid of CO2Interference effect in hypoxia-reoxygenation Mechanism Study；4. low-oxygen gas mixture body cabin, be also
Use singlechip technology and feedback principle, be automatically adjusted O in oxygen cabin2Concentration, provides low-oxygen gas mixture body and normal oxygen by interval
Gas, simulates intermittent hypoxia process, passes through rapid aeration simultaneously or places limestone CO absorption in nacelle2Maintain CO in nacelle2
It is maintained at low-level.
Said apparatus all can be automatically controlled by computer program can regulate the most quickly and easily hypoxia degree,
The parameter such as frequency and persistent period, meets in various degree and the requirement of various durations CIH, but said apparatus still suffers from one
Determine shortcoming, such as:
(1) one side air inlet, fast intermittent give oxygen concentration skewness in low-oxygen gas may cause cabin and cause every to move
In object, degree of oxygen deficiency is inconsistent, and in feeder defect causes hypoxia-reoxygenation process nacelle, pressure changes greatly, it is impossible to simulation is often
Pressure experimental state；
(2) the long-term monitoring in real time to every laboratory animal SaO2 is lacked, it is impossible to clear and definite whether modeling is successful；
(3) due to animal nacelle and the restriction of gas control equipment, can only carry out same group of experimental group modeling, time every time
Cost is high；
(4) the most above-mentioned experimental provision is all as the finger passing judgment on hypoxia degree by oxygen concentration in detection nacelle
Mark, and by oxygen concentration feedback regulation in nacelle for oxygen concentration, but in nacelle, oxygen concentration can not represent the reality in animal body
Oxygenate situation, body blood oxygen saturation depends primarily on arterial blood sample dividing potential drop, by alveolar ventilation, for multifactor shadows such as oxygen concentrations
Ringing, in nacelle, the factor such as uneven, the animal individual difference of oxygen concentration all may result in animal body, degree of oxygenation is different, makes with organizing reality
Test result and produce error, therefore in using nacelle, oxygen concentration as assessment laboratory animal degree of oxygen deficiency and does not meets physiological requirement.
Summary of the invention
In sum, in order to overcome the deficiencies in the prior art, the technical problem to be solved is to provide a kind of real
Time feedback multi-mode oxygen control system.
The technical scheme is that a kind of Real-time Feedback multi-mode oxygen control system, bag
Include compressed oxygen cylinder, compressed nitrogen gas cylinder, compressed air bottle, animal nacelle and single-chip computer control system, described Single-chip Controlling system
System includes that computer, single-chip microcomputer main frame, pressure transducer, oxygen concentration sensor, blood oxygen saturation probe and data process mould
Block, is provided with oxygen concentration sensor and pressure transducer in animal capsule body, each laboratory animal in described animal capsule body
The most all binding blood oxygen saturation probes, described blood oxygen saturation probe, pressure transducer and oxygen concentration sensor pass through data
Line connects the data processing module of described single-chip computer control system；Described data processing module is used for receiving, process and changing adopting
Air pressure, oxygen concentration and blood oxygen saturation initial data in the animal capsule body collected, and the data after converted are flowed to list
Sheet machine host carries out feedback regulation, is transported to computer through single-chip microcomputer the most again and shows through computer display screen and carry out in real time
Monitoring；
The outlet of described compressed oxygen cylinder by oxygen hose, described compressed nitrogen gas cylinder outlet by nitrogen tube and described
Compressed air bottle is pooled to air inlet pipe by air hose, and described air inlet pipe is separated into five different branch pipes respectively to described animal
Gas is provided in nacelle；
Described oxygen hose is provided with control oxygen and enters into the first gas control equipment in described animal capsule body, in institute
State described nitrogen tube be provided with control nitrogen enter into the second gas control equipment in described animal capsule body, at described air hose
Being provided with control air and enter into the 3rd gas control equipment in described animal capsule body, described single-chip computer control system passes through institute
State the first gas control equipment, the second gas control equipment and the 3rd gas control equipment to control respectively from described compressed oxygen
Bottle, described compressed nitrogen gas cylinder and described compressed air bottle be corresponding gas flow in being delivered to described animal capsule body, and described
Single-chip computer control system can be by regulating described first gas control equipment, described second gas control equipment and the 3rd gas
Control realize intermittent hypoxia pattern, Constant hypoxia pattern, normal oxygen pattern in device makes animal capsule body or continue hyperoxia pattern
Oxygen supply pattern, thus realize, under different oxygen environments, the animal in described animal capsule body is carried out different medical experiments.
The invention has the beneficial effects as follows: oxygen concentration in blood oxygen saturation, animal capsule body in this control system use animal body
And air pressure is as feedback information, by computer blood oxygen saturation in various degree needed for single-chip microcomputer sets or oxygen concentration and
Persistent period also inputs the single-chip microcomputer control program in single-chip computer control system, thus regulates each gas and enter in animal capsule body
Gas flow, be rapidly reached required oxygen environment in making animal capsule body, in making animal capsule body by multiple spot multiaspect supply simultaneously
Oxygen concentration is uniformly distributed rapidly, and then blood oxygen saturation keeps matching as far as possible and maintains preset range in making animal body, mould
Intend different oxygen environments and carry out medical experiment, the actual oxygenate situation in animal body can be monitored in real time simultaneously.
On the basis of technique scheme, the present invention can also do improvement further below:
Further, described first gas control equipment includes the first gas flowmeter, the first electromagnetic valve and first timer,
Described first gas flowmeter entrance connects the outlet of described compressed oxygen cylinder, and described first gas flowmeter exports by described
First electromagnetic valve connects described oxygen hose, described first electromagnetic valve is provided with timing controlled its be turned on or off described the
One intervalometer, described single-chip computer control system is by controlling the first electromagnetic valve described in described first timer time switch；
Described second gas control equipment includes the second gas flowmeter, the second electromagnetic valve and second timer, described
Two gas flowmeter entrances connect the outlet of described compressed nitrogen gas cylinder, and described second gas flowmeter outlet is by described second electricity
Magnet valve connects described nitrogen tube, is provided with its described second timing being turned on or off of timing controlled on described second electromagnetic valve
Device, described single-chip computer control system is by controlling the second electromagnetic valve described in described second timer time switch；
Described 3rd gas control equipment includes the 3rd gas flowmeter and the 3rd electromagnetic valve, described 3rd gas flowmeter
Export and connect described air hose by described 3rd electromagnetic valve.
Use having the beneficial effect that by corresponding gas control equipment regulation input animal of above-mentioned further technical scheme
The input flow rate of corresponding gas in nacelle, thus realize providing different for oxygen concentration to animal nacelle, final simulation is different
Oxygen environment carries out medical experiment.
Further, described animal nacelle is cube structure, and five described branch roads are respectively from four sides of described animal nacelle
Face and end face are communicated in animal capsule body.
Use above-mentioned further technical scheme has the beneficial effect that multiaspect supply makes gas the most uniform in animal capsule body
Distribution.
Further, it is external that the bottom surface of described animal nacelle can pull out animal capsule, is provided with in the side of described animal nacelle
For the window that its inside is operated.
Having the beneficial effect that of above-mentioned further technical scheme is used to be easy to the cleaning in portion in animal capsule body and operation.
Further, it is provided with the gas outlet for discharging its internal gas, described gas outlet in the side of described animal nacelle
Exhaust flow-meter and exhaust solenoid valve is connected by pipeline.
Having the beneficial effect that of above-mentioned further technical scheme is used to make the air pressure in described animal capsule body remain at often
Pressure condition.
Further, it is placed with the calcium carbonate for absorbing carbon dioxide in described animal capsule body.
Use above-mentioned further technical scheme has the beneficial effect that CO in maintenance animal capsule body2In low-level, get rid of animal
High CO in nacelle2Impact on experiment.
Further, described animal nacelle is provided with multiple, and is provided with the first gas one to one with described animal nacelle
Control device, the second gas control equipment and the 3rd gas control equipment.
Use above-mentioned further technical scheme has the beneficial effect that each animal nacelle carries out a treated animal experiment, can be same
The zoopery of many groups of same experiment parameters of Shi Jinhang, also can carry out organizing the zoopery of different experiments parameter more simultaneously.
Further, described single-chip computer control system also includes that blood oxygen saturation alarm device, described computer can arrange institute
State the alarming value of alarm device.
Having the beneficial effect that of above-mentioned further technical scheme is used to avoid because individual variation causes individual animal severe depletion of oxygen
Dead.
Further, described single-chip computer control system also include being respectively used to detection and show described first electromagnetic valve, second
The display lamp that electromagnetic valve and the 3rd electromagnetic valve are turned on or off.
The useful benefit using above-mentioned further technical scheme is: by observing the closed mode of corresponding display lamp, in real time
In monitoring animal capsule body, oxygen, nitrogen and air are for gaseity.
Accompanying drawing explanation
Fig. 1 is the connection diagram (an animal nacelle, and remove single-chip computer control system) of the present invention；
Fig. 2 is principle of the invention control block diagram (two animal nacelle).
In accompanying drawing, the list of parts representated by each label is as follows:
1, compressed oxygen cylinder, 2, compressed nitrogen gas cylinder, 3, compressed air bottle, 4, animal nacelle, the 5, first gas flowmeter, 6,
First timer, the 7, first electromagnetic valve, 8, oxygen hose, the 9, second gas flowmeter, 10, second timer, the 11, second electromagnetism
Valve, 12, nitrogen tube, the 13, the 3rd gas flowmeter, the 14, the 3rd electromagnetic valve, 15, air hose, 16, air inlet pipe, 18, gas outlet,
17, exhaust flow-meter, 19, exhaust solenoid valve.
Detailed description of the invention
Being described principle and the feature of the present invention below in conjunction with accompanying drawing, example is served only for explaining the present invention, and
Non-for limiting the scope of the present invention.
As it is shown in figure 1, a kind of Real-time Feedback multi-mode oxygen control system, including compressed oxygen cylinder 1, compressed nitrogen gas cylinder 2, pressure
Contracting air bottle 3, animal nacelle 4 and single-chip computer control system, described single-chip computer control system include computer, single-chip microcomputer main frame,
Pressure transducer, oxygen concentration sensor, blood oxygen saturation probe and data processing module, arrange aerobic in animal nacelle 4
Concentration sensor and pressure transducer, all bind blood oxygen saturation with each laboratory animal in described animal nacelle 4 and visit
Head, described blood oxygen saturation probe, pressure transducer and oxygen concentration sensor connect described Single-chip Controlling system by data wire
The data processing module of system；Described data processing module is for receiving, process and change pressure, oxygen concentration and the blood oxygen collected
Saturation initial data, and the data after converted are flowed to single-chip microcomputer main frame carry out feedback regulation, simultaneously again through single-chip microcomputer
It is transported to computer show in real time through computer display screen.
It is placed with the calcium carbonate for absorbing carbon dioxide in described animal nacelle 4, absorbs a part of CO by calcium carbonate2
CO in rear maintenance animal nacelle 42In low-level, get rid of high CO in animal nacelle 42Impact on experiment.
Described compressed oxygen cylinder 1 outlet by oxygen hose 8, described compressed nitrogen gas cylinder 2 outlet by nitrogen tube 12 with
And described compressed air bottle 3 is pooled to air inlet pipe 16 by air hose 15, described air inlet pipe 16 is separated into five different branch pipes
Gas is provided respectively in described animal nacelle 4.Described animal nacelle 4 is cube structure, and five described branch roads are respectively from described
Four sides of animal nacelle 4 and end face are communicated in animal nacelle 4.The bottom surface of described animal nacelle 4 can pull out animal
Outside nacelle 4, it is provided with the window for its inside is operated in the side of described animal nacelle 4.In described animal nacelle 4
Side is provided with the gas outlet 18 for discharging its internal gas, and described gas outlet 18 connects exhaust flow-meter 17 and row by pipeline
Pneumoelectric magnet valve 19, described single-chip computer control system is by regulation and controls described exhaust flow-meter 17 and exhaust solenoid valve 19 controls
Flow of exhaust amount, and then make the air pressure in described animal nacelle 4 remain at atmospheric pressure state.As in figure 2 it is shown, according to reality
Needing, described animal nacelle 4 is provided with one or more, and is provided with the first gas one to one with described animal nacelle 4
Control device, the second gas control equipment and the 3rd gas control equipment.Arranged by Single Chip Microcomputer (SCM) program and carry out different oxygen supply
The zoopery of environment, can carry out one or more groups zoopery simultaneously.
Described oxygen hose 8 is provided with control oxygen and enters into the first gas control equipment in described animal nacelle 4,
Described nitrogen tube 12 is provided with control nitrogen and enters into the second gas control equipment in described animal nacelle 4, at described air
Pipe 15 is provided with control air and enters into the 3rd gas control equipment in described animal nacelle 4.First gas control equipment,
Two gas control equipments and the 3rd gas control equipment are specific as follows:
Described first gas control equipment includes the first gas flowmeter 5, first timer 6 and the first electromagnetic valve 7, described
First gas flowmeter 5 entrance connects the outlet of described compressed oxygen cylinder 1, and described first gas flowmeter 5 exports by described
First electromagnetic valve 7 connects described oxygen hose 8, is provided with its institute being turned on or off of timing controlled on described first electromagnetic valve 7
Stating first timer 6, described single-chip computer control system is by controlling the first electromagnetic valve described in described first timer 6 time switch
7；
Described second gas control equipment includes the second gas flowmeter 9, second timer 10 and the second electromagnetic valve 11, institute
Stating the second gas flowmeter 9 entrance and connect the outlet of described compressed nitrogen gas cylinder 2, described second gas flowmeter 9 exports by institute
Stating the second electromagnetic valve 11 and connect described nitrogen tube 12, it is turned on or off to be provided with timing controlled on described second electromagnetic valve 11
Described second timer 10, described single-chip computer control system is by controlling described in described second timer 10 time switch second
Electromagnetic valve 11；
Described 3rd gas control equipment includes the 3rd gas flow 13 meter and the 3rd electromagnetic valve 14, described 3rd gas stream
Gauge 13 exports and connects described air hose 15 by described 3rd electromagnetic valve 14.
Described single-chip computer control system is by described first gas control equipment, the second gas control equipment and the 3rd gas
Control device to control respectively to be delivered to institute from described compressed oxygen cylinder 1, described compressed nitrogen gas cylinder 2 and described compressed air bottle 3
Corresponding gas flow in stating animal nacelle 4, and described single-chip computer control system is by the described first gas control dress of regulation
Put, described second gas control equipment, described 3rd gas control equipment can make the environment in animal capsule body 4 become the lowest
Oxygen, Constant hypoxia, normal oxygen or the multiple oxygen environment of lasting hyperoxia, thus realize under different oxygen environments described animal
Animal in nacelle 4 carries out medical experiment.Described single-chip computer control system also includes being respectively used to detection and showing described first
The display lamp that electromagnetic valve the 7, second electromagnetic valve 11 and the 3rd electromagnetic valve 14 are turned on or off, by observing corresponding display lamp
Closed mode, in monitoring animal capsule body, oxygen, nitrogen and air, for gaseity, when corresponding display lamp lights, represent in real time
Corresponding electromagnetic valve is opening, supply in i.e. corresponding gas forward animal capsule body.
This control system is programmed through the PLC program of single-chip computer control system, can be to various gas flows and time
It is controlled, intermittent hypoxia pattern, normal oxygen pattern, Constant hypoxia pattern, the multiple-working mode of hyperoxia pattern can be performed, below
It is described separately:
(1) intermittent hypoxia pattern implementation example:
Experiment is preset: single-chip computer control system presets the blood oxygen saturation desired value of animal in this test group of animals nacelle 4
Scope is 82%-85%, and second timer 10 working time controlling nitrogen supply is 30s, and the intermittent time is 40s, i.e. hypoxia
Interval is 30s, and normal oxygen interval is 40s.
Embodiment:
1. by the first timer 6 of single-chip computer control system closing control oxygen control supply, and blood oxygen saturation is set
Target range is 82%-85%, and second timer 10 working time controlling nitrogen supply is 30s, and the intermittent time is
40s------parameter is arranged；
2., after starting the intermittent hypoxia model program of single-chip computer control system, second timer 10 He of nitrogen supply is controlled
Second electromagnetic valve 11 is opened, rapidly supplying nitrogen in animal nacelle 4, and exhaust solenoid valve 19 is also opened simultaneously, Single-chip Controlling
Data that system detects according to the blood oxygen saturation inspection probe with animal and the data regulation that oxygen concentration sensor detects
Control the second gas flowmeter 9 of nitrogen supply, thus control to input the nitrogen flow in animal nacelle 4, according to animal nacelle 4
The data regulation extraction flow that pressure sensor detects is constant with pressure in maintaining animal nacelle 4, in making animal nacelle 4
Oxygen concentration declines rapidly, and in making animal nacelle 4 with the fastest speed, the blood oxygen saturation of each animal drops to preset range, and most
May keep that matching-------this is nitrogen conveying process, i.e. realize hypoxia；
3. when in animal nacelle 4 animal blood oxygen saturation drop to preset level, and control nitrogen supply after tending to matching
The second electromagnetic valve 11 and exhaust solenoid valve 19 close, maintain hypoxia 30s------hypoxia in animal nacelle 4 to maintain rank
Section, i.e. hypoxia interval；
4. the second timer 10 controlling nitrogen supply after reaching 30s is automatically switched off, and controls the second of nitrogen supply simultaneously
Electromagnetic valve 11 is also automatically switched off, now control oxygen supply the first electromagnetic valve 7 and exhaust solenoid valve 19 automatic opening, start to
Conveying oxygen in animal nacelle 4, in making animal nacelle 4 with the fastest speed, oxygen concentration recovers to normal oxygen condition, makes animal blood oxygen
Saturation is recovered to normal level------reoxygenation process；
5. the first electromagnetic valve 7 controlling oxygen supply after oxygen concentration reaches normal oxygen condition in animal nacelle 4 closes automatically
Closing, the 3rd electromagnetic valve 14 of control air supply is open, starts to carry air in animal nacelle 4, in maintenance animal nacelle 4 often
Oxygen condition, reopens------often oxygen and maintains rank after reaching 40s until second timer 10 intermittent time controlling nitrogen supply
Section, i.e. often oxygen interval；
The second electromagnetic valve 11 and the exhaust solenoid valve 19 that the most now control nitrogen supply open, again again to animal nacelle
Supplying nitrogen in 4, the most repeatedly, repeats supplying nitrogen-oxygen-air, to reach the circulation-----the of the oxygen of hypoxia-reoxygenation-often
Secondary cycle.
If it addition, there is multiple animal nacelle 4, each animal nacelle 4 working method is all by single-chip computer control system
Independent control, can perform different experiment parameters, as other animal nacelle 4 requires that presetting blood oxygen saturation desired value is 65%-
68%%, the working time of the second timer 10 controlling nitrogen supply is 40s, and the intermittent time is 50s, and by single-chip microcomputer journey
Sequence designs, and can send different experiment parameter instructions, and different animal nacelle 4 performs different experiment parameters simultaneously, i.e. same
The zoopery that Shi Jinhang many groups experiment parameter is different.
(2) normal oxygen pattern
The simplest, in addition to air controls electromagnetic valve and exhaust solenoid valve, other switches are turned off, and continue toward defeated in nacelle
Send air, can be as experiment contrast group pattern.
(3) Constant hypoxia pattern
Experiment is preset: in default animal nacelle 4, animal blood oxygen saturation target range is 70%-73%, and hypoxia is lasting
Time is 3h.
Embodiment:
1. by the first timer 6 of single-chip computer control system closing control oxygen supply, set and preset blood oxygen saturation
Target range is 70%-73%, and the working time arranging the second timer 10 controlling nitrogen supply is 3h-----parameter
Arrange；
2., after starting the Constant hypoxia model program of single-chip computer control system, the second electromagnetic valve 11 He of nitrogen supply is controlled
Second timer 10 opens simultaneously, starts supplying nitrogen in animal nacelle 4, and in animal nacelle 4, oxygen concentration declines rapidly, so that
Animal blood oxygen saturation is rapidly reached preset range, and tends to matching as far as possible, and exhaust solenoid valve 19 also opens simultaneously, to maintain
Pressure stability maintain atmospheric pressure state------realizes hypoxia in animal nacelle 4；
The first electromagnetic valve 7 the most now controlling oxygen supply is open, carries oxygen and nitrogen in animal nacelle 4 simultaneously,
Single-chip computer control system regulates oxygen and the transportation flow of nitrogen according to the blood oxygen saturation of oxygen concentration and animal in animal nacelle 4
Amount, makes the blood oxygen saturation of animal in animal nacelle 4 maintain preset level all the time, until controlling the second timing of nitrogen supply
The working time of device 10 reach 3h after system be automatically switched off------hypoxia maintenance stage.
(4) hyperoxia pattern is continued
Experiment is preset: in nacelle, oxygen concentration is 80%, and hyperoxia is held time as 3h
Embodiment:
1. controlled the second timer 10 of nitrogen supply by the stop of single-chip computer control system, and set default oxygen
Concentration is 80%, and the working time of the first timer 6 of setup control oxygen supply is that 3h-----parameter is arranged；
2. after starting the lasting hyperoxia model program of single-chip computer control system, control oxygen supply the first electromagnetic valve 7 and
Exhaust solenoid valve 19 is open, starts to carry oxygen in animal nacelle 4, and in making animal nacelle 4, oxygen concentration is rapidly reached default water
Flat, exhaust solenoid valve 19 is open simultaneously, to maintain in animal nacelle 4 pressure stability and to maintain atmospheric pressure state-----realizes height
Oxygen process；
The second electromagnetic valve 11 the most now controlling nitrogen supply is opened, simultaneously supplying nitrogen and oxygen in animal nacelle 4,
Single-chip microcomputer control program is according to oxygen concentration regulation oxygen and the feed flow of nitrogen in animal nacelle 4, and in making animal nacelle 4, oxygen is dense
Degree maintains preset level all the time, and after reaching 3h until the working time controlling the first timer 6 that oxygen supplies, system is automatic
Close-----hyperoxia maintenance stage.
In sum, this system has the advantage that
1, fast intermittent gives oxygen concentration skewness in low-oxygen gas may cause cabin and causes anoxia in every animal body
Degree is inconsistent, by steam line and the improvement of animal nacelle, it is achieved multiaspect multiple spot supplies, and in making nacelle, gas divides rapidly
Cloth is uniform, uses blood oxygen saturation and oxygen concentration as feedback information simultaneously, feeds back to single-chip computer control system, according to default blood
Oxygen saturation or oxygen concentration levels carry out gas supply flow regulation, the same blood oxygen saturation organizing laboratory animal in making same animal capsule body
Tend to matching, and be maintained at preset range, reduce with organizing experimental error；
2, fast intermittent oxygen supply causes nacelle internal gas pressure unstable, is fed back by pressure transducer and the tune of exhaust solenoid valve
Joint, in making nacelle, pressure remains at atmospheric pressure state；
3, lack every laboratory animal SaO2Long-term monitoring in real time, it is impossible to clear and definite whether modeling success, this system with
Blood oxygen saturation, as real-time feedback information, represents the actual oxygenate situation in animal body, carries out than oxygen concentration in detection nacelle
Degree of oxygen deficiency assessment is more accurate, and can instant clear and definite modeling whether success；
4, due to animal nacelle and the restriction of gas control equipment, can only carry out same group of experimental group modeling, time every time
Cost is high, and this system is by improving, by setting difference experiment nacelle, gas control equipment, PLC program programming etc. are many-sided
The blood oxygen saturation of degree or oxygen concentration and persistent period, can realize the air supply requirement of various modes, different animals nacelle can be with
The zoopery of mono-group of identical experiment parameter of Shi Jinhang, also can carry out many treated animals experiment of different experiments parameter simultaneously, shorten
The modeling time, save time cost；
5, the PLC control program of single-chip computer control system can arrange blood oxygen saturation alarm device, can set different range
Alarming value, it is to avoid because individual variation causes individual animal severe depletion of oxygen dead.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all spirit in the present invention and
Within principle, any modification, equivalent substitution and improvement etc. made, should be included within the scope of the present invention.
Claims (9)
1. a Real-time Feedback multi-mode oxygen control system, it is characterised in that include compressed oxygen cylinder (1), compressed nitrogen gas cylinder
(2), compressed air bottle (3), animal nacelle (4) and single-chip computer control system, described single-chip computer control system includes computer, list
Sheet machine host, pressure transducer, oxygen concentration sensor, blood oxygen saturation probe and data processing module, in animal nacelle (4)
Inside it is provided with oxygen concentration sensor and pressure transducer, all binds with each laboratory animal in described animal nacelle (4)
Blood oxygen saturation is popped one's head in, and described blood oxygen saturation probe, pressure transducer and oxygen concentration sensor connect described by data wire
The data processing module of single-chip computer control system；Described data processing module is for receiving, process and change the animal collected
The air pressure of nacelle (4), oxygen concentration and blood oxygen saturation initial data, and the data after converted are flowed to single-chip microcomputer main frame enter
Row feedback regulation, is transported to computer through single-chip microcomputer main frame more simultaneously and shows in real time through computer display screen；
The outlet of described compressed oxygen cylinder (1) passes through nitrogen tube by (2) outlet of oxygen hose (8), described compressed nitrogen gas cylinder
(12) and described compressed air bottle (3) is pooled to air inlet pipe (16) by air hose (15), described air inlet pipe (16) is separated into
Five different branch pipes provide gas respectively in described animal nacelle (4)；
Described oxygen hose (8) is provided with control oxygen and enters into the first gas control equipment in described animal nacelle (4),
Described nitrogen tube (12) is provided with control nitrogen and enters into the second gas control equipment in described animal nacelle (4),
Described air hose (15) is provided with control air and enters into the 3rd gas control equipment in described animal nacelle (4), described list
Sheet machine control system is distinguished by described first gas control equipment, the second gas control equipment and the 3rd gas control equipment
Control to be delivered to described animal capsule from described compressed oxygen cylinder (1), described compressed nitrogen gas cylinder (2) and described compressed air bottle (3)
Corresponding gas flow in body (4), and described single-chip computer control system can by regulate described first gas control equipment,
Described second gas control equipment and the 3rd gas control equipment present intermittent hypoxia, Constant hypoxia, often in making animal nacelle (4)
Oxygen pattern or the multiple oxygen environment of lasting hyperoxia, thus realize under different oxygen environments in described animal nacelle (4)
Animal carry out different medical experiments.
Real-time Feedback multi-mode oxygen control system the most according to claim 1, it is characterised in that described first gas controls
Device includes the first gas flowmeter (5), first timer (6) and the first electromagnetic valve (7), described first gas flowmeter (5)
Entrance connects the outlet of described compressed oxygen cylinder (1), and described first gas flowmeter (5) exports by described first electromagnetic valve
(7) connect described oxygen hose (8), described first electromagnetic valve (11) is provided with timing controlled its be turned on or off described
One intervalometer (6), described single-chip computer control system is by controlling the first electromagnetic valve (7) described in described intervalometer (6) time switch；
Described second gas control equipment includes the second gas flowmeter (9), second timer (10) and the second electromagnetic valve (11),
Described second gas flowmeter (9) entrance connects the outlet of described compressed nitrogen gas cylinder (2), and described second gas flowmeter (9) goes out
Mouth connects described nitrogen tube (12) by described second electromagnetic valve (11), is provided with timing controlled on described second electromagnetic valve (11)
The described second timer (10) that it is turned on or off, described single-chip computer control system is fixed by controlling second timer (10)
Second electromagnetic valve (11) described in Shi Kaiguan；
Described 3rd gas control equipment includes the 3rd gas flow (13) meter and the 3rd electromagnetic valve (14), described 3rd gas stream
Gauge (13) outlet connects described air hose (15) by described 3rd electromagnetic valve (14).
Real-time Feedback multi-mode oxygen control system the most according to claim 1, it is characterised in that described animal nacelle (4)
For cube structure, five described branch roads are communicated to animal nacelle from four sides of described animal nacelle (4) and end face respectively
(4) in.
Real-time Feedback multi-mode oxygen control system the most according to claim 3, it is characterised in that described animal nacelle (4)
Bottom surface can pull out animal nacelle (4) outward, be provided with in the side of described animal nacelle (4) for its inside is operated
Window.
Real-time Feedback multi-mode oxygen control system the most according to claim 1, it is characterised in that in described animal nacelle
(4) side is provided with the gas outlet (18) for discharging its internal gas, and described gas outlet (18) connect exhaust stream by pipeline
Gauge (17) and exhaust solenoid valve (19), described single-chip computer control system by regulation and control described exhaust flow-meter (17) and
Exhaust solenoid valve (19) controls to discharge the flow of gas, and then makes the air pressure in described animal nacelle (4) remain at normal pressure
State.
Real-time Feedback multi-mode oxygen control system the most according to claim 1, it is characterised in that described animal nacelle (4)
Inside it is placed with the calcium carbonate for absorbing carbon dioxide.
7. according to claim 1 as the Real-time Feedback multi-mode oxygen control system described in 6 any one, it is characterised in that described
Animal nacelle (4) is provided with multiple, and with described animal nacelle (4) be provided with one to one the first gas control equipment, second
Gas control equipment and the 3rd gas control equipment.
8. according to claim 1 as the Real-time Feedback multi-mode oxygen control system described in 6 any one, it is characterised in that described
Single-chip computer control system also includes the alarm device detecting blood oxygen saturation, and described computer can arrange the report of described alarm device
Alert value.
9. according to claim 1 as the Real-time Feedback multi-mode oxygen control system described in 6 any one, it is characterised in that described
Single-chip computer control system also includes being respectively used to detection and shows described first electromagnetic valve (7), the second electromagnetic valve (11) and the
The display lamp that three electromagnetic valves (14) are turned on or off.
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107126212A (en) * | 2017-06-16 | 2017-09-05 | 中国科学院亚热带农业生态研究所 | Intelligent animals respiratory metabolism determining system |
| CN108458251A (en) * | 2018-06-08 | 2018-08-28 | 周先林 | Feeding mechanism containing oxygen gas mixture and intermittence low-oxygen Supply Method |
| CN109172213A (en) * | 2018-10-04 | 2019-01-11 | 同惠生物科技（东莞）有限公司 | Intelligent animals oxygen cabin and its monitoring method |
| CN109223391A (en) * | 2018-10-04 | 2019-01-18 | 同惠生物科技（东莞）有限公司 | Information-based animal oxygen cabin and its approaches to IM |
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| CN112790889A (en) * | 2021-02-01 | 2021-05-14 | 成都森威实验动物有限公司 | Preparation method and system of mixed gas for preparing intermittent hypoxia animal model |
| CN113180967A (en) * | 2021-04-30 | 2021-07-30 | 兵器工业卫生研究所 | Normal-pressure low-oxygen pre-study clothes training method and system |
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4107818A (en) * | 1975-12-11 | 1978-08-22 | Universities Federation For Animal Welfare | Animal euthanasia |
| CN1154611A (en) * | 1995-12-19 | 1997-07-16 | 现代电子产业株式会社 | Echo removing system |
| CN101584610A (en) * | 2009-06-12 | 2009-11-25 | 温州医学院 | Intermittent hypoxia big and small mice feeding cabin of loop track type |
| CN201409995Y (en) * | 2009-06-08 | 2010-02-24 | 中国人民解放军第三军医大学第二附属医院 | Full-automatic constant pressure low oxygen high carbon clean animal culture cabin |
| CN201631420U (en) * | 2010-02-03 | 2010-11-17 | 公安部警犬技术学校 | Atomization therapeutic instrument for respiratory disease of small animal |
| CN101889908A (en) * | 2010-07-06 | 2010-11-24 | 首都医科大学附属北京朝阳医院 | Gas atomization toxicant exposure box |
| DE202010009330U1 (en) * | 2010-06-21 | 2011-10-20 | Ai Mediq S.A. | Device for complex hypoxia therapy |
| CN102670326A (en) * | 2012-05-18 | 2012-09-19 | 温州医学院附属第二医院 | Compound simulating cabin for rat and mice tests and control method thereof |
| CN104885954A (en) * | 2015-04-07 | 2015-09-09 | 贾正平 | A construction method for a rat hypoxia simulation model of a highland low pressure hypoxia simulation chamber |
| CN206303994U (en) * | 2016-08-29 | 2017-07-07 | 叶贤伟 | A kind of Real-time Feedback multi-mode oxygen control system |
-
2016
- 2016-08-29 CN CN201610752630.3A patent/CN106137453B/en not_active Expired - Fee Related
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4107818A (en) * | 1975-12-11 | 1978-08-22 | Universities Federation For Animal Welfare | Animal euthanasia |
| CN1154611A (en) * | 1995-12-19 | 1997-07-16 | 现代电子产业株式会社 | Echo removing system |
| CN201409995Y (en) * | 2009-06-08 | 2010-02-24 | 中国人民解放军第三军医大学第二附属医院 | Full-automatic constant pressure low oxygen high carbon clean animal culture cabin |
| CN101584610A (en) * | 2009-06-12 | 2009-11-25 | 温州医学院 | Intermittent hypoxia big and small mice feeding cabin of loop track type |
| CN201631420U (en) * | 2010-02-03 | 2010-11-17 | 公安部警犬技术学校 | Atomization therapeutic instrument for respiratory disease of small animal |
| DE202010009330U1 (en) * | 2010-06-21 | 2011-10-20 | Ai Mediq S.A. | Device for complex hypoxia therapy |
| CN101889908A (en) * | 2010-07-06 | 2010-11-24 | 首都医科大学附属北京朝阳医院 | Gas atomization toxicant exposure box |
| CN102670326A (en) * | 2012-05-18 | 2012-09-19 | 温州医学院附属第二医院 | Compound simulating cabin for rat and mice tests and control method thereof |
| CN104885954A (en) * | 2015-04-07 | 2015-09-09 | 贾正平 | A construction method for a rat hypoxia simulation model of a highland low pressure hypoxia simulation chamber |
| CN206303994U (en) * | 2016-08-29 | 2017-07-07 | 叶贤伟 | A kind of Real-time Feedback multi-mode oxygen control system |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107126212A (en) * | 2017-06-16 | 2017-09-05 | 中国科学院亚热带农业生态研究所 | Intelligent animals respiratory metabolism determining system |
| CN108458251A (en) * | 2018-06-08 | 2018-08-28 | 周先林 | Feeding mechanism containing oxygen gas mixture and intermittence low-oxygen Supply Method |
| CN109172213B (en) * | 2018-10-04 | 2023-08-22 | 同惠生物科技（东莞）有限公司 | Intelligent animal oxygen cabin and monitoring method thereof |
| CN109172213A (en) * | 2018-10-04 | 2019-01-11 | 同惠生物科技（东莞）有限公司 | Intelligent animals oxygen cabin and its monitoring method |
| CN109223391A (en) * | 2018-10-04 | 2019-01-18 | 同惠生物科技（东莞）有限公司 | Information-based animal oxygen cabin and its approaches to IM |
| CN109444218A (en) * | 2018-12-17 | 2019-03-08 | 西南科技大学 | Improved ambient condition simulator and application method in a kind of reaction of oxidation kinetics |
| CN109444218B (en) * | 2018-12-17 | 2024-02-20 | 西南科技大学 | Improved environment state simulation device in oxidation kinetics reaction and use method |
| CN109350298A (en) * | 2018-12-20 | 2019-02-19 | 天津市胸科医院 | Realize the ventilation adjusting device of intermittent airway obstruction |
| CN109350298B (en) * | 2018-12-20 | 2020-12-22 | 天津市胸科医院 | Ventilation regulating and controlling equipment for realizing intermittent airway obstruction |
| CN110093256A (en) * | 2019-05-23 | 2019-08-06 | 华东理工大学 | A kind of automatic dissolved oxygen cyclic fluctuation device |
| CN110141396A (en) * | 2019-05-24 | 2019-08-20 | 四川大学 | A kind of hypoxia device for clinical zoopery |
| CN110721044A (en) * | 2019-10-17 | 2020-01-24 | 上海塔望智能科技有限公司 | Hyperbaric oxygen chamber for animal experiments |
| CN111920548A (en) * | 2020-08-18 | 2020-11-13 | 南通大学 | Argon-oxygen gas animal experiment hyperbaric chamber and animal experiment method thereof |
| CN112790889A (en) * | 2021-02-01 | 2021-05-14 | 成都森威实验动物有限公司 | Preparation method and system of mixed gas for preparing intermittent hypoxia animal model |
| CN113180967A (en) * | 2021-04-30 | 2021-07-30 | 兵器工业卫生研究所 | Normal-pressure low-oxygen pre-study clothes training method and system |
| CN113812347A (en) * | 2021-09-06 | 2021-12-21 | 中国人民解放军海军特色医学中心 | Suit cabin adds decompression system |
| CN117378514A (en) * | 2023-11-29 | 2024-01-12 | 中国人民解放军海军特色医学中心 | Animal experiment system under high-pressure environment |
| CN117378514B (en) * | 2023-11-29 | 2024-04-02 | 中国人民解放军海军特色医学中心 | Animal experiment system under high-pressure environment |
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