CN106117319A - Biological extraction method produces medicinal pituitrin technique - Google Patents
Biological extraction method produces medicinal pituitrin technique Download PDFInfo
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- CN106117319A CN106117319A CN201610481617.9A CN201610481617A CN106117319A CN 106117319 A CN106117319 A CN 106117319A CN 201610481617 A CN201610481617 A CN 201610481617A CN 106117319 A CN106117319 A CN 106117319A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/16—Oxytocins; Vasopressins; Related peptides
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Abstract
The invention discloses biological extraction method and produce medicinal pituitrin technique, production technology includes: just take off acetone, separation, prepared by posterior lobe coarse powder, prepared by ether posterior lobe powder, prepared by Powdered Posterior Pituitary, prepared by extracting solution, deproteinization, packs, stores, and present invention process method is advanced, from pig cerebellum, extract medicinal pituitrin by biological extraction method, there is the advantages such as extraction efficiency height, clarity height, quality better, long shelf-life.
Description
Technical field
The invention belongs to biological pharmacy technical field, be specifically related to biological extraction method and produce medicinal pituitrin technique.
Background technology
Nineteen twenty separates from posterior lobe and two kinds of hormones of Partial purification, pituitrin and vassopressin.They are all by mound
The neurocyte secretion that brain bottom is special, arrive posterior lobe along hypothalamus hypophysis bundle and be stored in posterior lobe.
Pituitrin is the mixture of oxytocin and vassopressin, and its effective ingredient titer ratio is 0.9～1.7；Mainly
For lung, bronchorrhagia (as spitting of blood) digestive tract hemorrhage (hematemesis, have blood in stool).And be applicable to gynecological hasten parturition and puerperal shrink son
Palace, hemostasis etc..The most powerful for abdominal operation rear intestinal paralysis etc..Pituitrin still diabetes insipidus is reduced voided volume it
Effect.Posterior pituitary injection has strong contraction effect to smooth muscle, especially acts on higher with the basic unit to blood vessel and uterus, due to
Dosage is different, and the uterus rhythm and pace of moving things can be caused to be contracted to tetanic contraction.Also can increase tension force for intestinal and bladder and make it shrink.This
Outward, pituitrin still can suppress to urinate.
PH value between 3.0～4.0 the most stable, 90 DEG C of heating half an hour or less than 50 DEG C long periods heat and do not lose
Live.When pH=5.0, heating can cause the loss of titer；During pH=6.0, the work that the most also loss of energy is more
Power.Room temperature is placed 24 hours in glacial acetic acid, and change is can not check in inspection.
And existing pituitrin production technology often uses, and to there is pituitrin internal protein impurity content high；Carry
Take efficiency low；Prior art pituitrin generally uses unsterilised or half aseptic tank to be honored as a queen flowing steam sterilizing, causes hypophysis
Pituitrin clarity is relatively low, and apt to deteriorate, inefficacy.
Summary of the invention
It is an object of the invention to overcome above-mentioned the deficiencies in the prior art, it is provided that biological extraction method produces medicinal lobus posterior hypophyseos
Element technique.
Biological extraction method produces medicinal pituitrin technique, comprises the following steps:
1) acetone, separation are just taken off
Take out the pig cerebellum being soaked in acetone, count in being placed in dish, remove broken pig cerebellum, granulate pig cerebellum
It is separated quickly into frontal lobe and posterior lobe with tweezers, and posterior lobe is placed in acetone soln immersion；
2) prepared by posterior lobe coarse powder
From acetone soln, take out posterior lobe, and be filtered dry on funnel with gauze, after acetone volatilization to the greatest extent, put into drying baker
In, it being dried 5～7 days, dried dry posterior lobe is sent into reducing mechanism and is ground into posterior lobe powder, and posterior lobe powder crosses 40～60 mesh sieves, collects
Posterior lobe coarse powder is also weighed；
3) prepared by ether posterior lobe powder
In posterior lobe coarse powder, add the cold acetone of 3 times of posterior lobe coarse powder amounts, be stirred at room temperature 1.5 hours, stand 6～12 hours,
Pouring out supernatant, precipitate adds the cold acetone of 1 times of precipitate amount, same treatment three times again, drains for the last time, and precipitate is third
Ketone posterior lobe powder, adds the cold diethyl ether of 3 times of acetone posterior lobe powder amounts in acetone posterior lobe powder, is stirred at room temperature 1 hour, after standing 30 minutes
Filter, volatilization ether to the greatest extent, obtain ether posterior lobe powder；
4) prepared by Powdered Posterior Pituitary
Ether posterior lobe powder is put in drying baker, is dried 5～7 days, takes out, obtain hypophysis after moisture is reduced to below 6%
Posterior lobe powder, Powdered Posterior Pituitary sterilization, the sealing of clean film bundling, tagged state mark is placed the cryostat of less than 5 DEG C and is protected
Deposit；
5) prepared by extracting solution
Weighing Powdered Posterior Pituitary 3000g, once extract, the step once extracted is: add in Powdered Posterior Pituitary
Concentration is 0.25% acetum 150000mL, stirs, extracts 15 minutes under 60～65 DEG C of temperature conditionss, Powdered Posterior Pituitary
The cold place of extracting solution stands 6～12 hours, the cold filter of extracting solution buchner funnel after standing, obtains extracting solution and filtering residue for the first time,
Adding concentration in filtering residue is 0.25% acetum 60000mL, carries out second extraction, the time of second extraction, temperature and filtration
Mode with once extract identical, obtain second time extracting solution, extracting solution is mixed to get extracting solution with for the first time extracting solution for the second time；
6) deproteinization
In extracting solution add ten thousand/ activated carbon, be heated rapidly to 100 DEG C be incubated 30 minutes, take out be positioned over-5
Place 6～12 hours under temperature conditions below DEG C, take out coolant, first with paper pulp funnel sucking filtration, then through 30kD ultrafilter membrane mistake
Filter filter sucking filtration, to clear and bright, obtains pituitary solution, and pituitary solution is sampled inspection；
7) pack, store
Pituitary solution after the assay was approved is sub-packed in the stainless steel cask of cleaning, the plastic foil wrapping that bung is clean
Seal, label outside bucket, be placed in less than-5 DEG C and deposit.
Further, the proportion of described cold acetone is 0.820～0.830, and the temperature of cold acetone is 15 DEG C.
Further, described pulverizer is pottery pulverizer or ball milling altar.
Further, described drying baker all uses dry lime as desiccant.
Technical solution of the present invention biological extraction method produces medicinal pituitrin technique, takes out the pig being soaked in acetone little
Brain, counts in being placed in dish, removes broken pig cerebellum, and granulate pig cerebellum tweezers are separated quickly into frontal lobe and posterior lobe, and
Posterior lobe is placed in acetone soln immersion, broken pig cerebellum and frontal lobe is gone divided by the quality improving pituitrin finished product；
Prepare posterior lobe coarse powder so that the follow-up precipitation without composition and effectiveness；The cold acetone of 3 times of posterior lobe coarse powder amounts is added in posterior lobe coarse powder,
Being stirred at room temperature 1.5 hours, stand 6～12 hours, pour out supernatant, precipitate adds the cold acetone of 1 times of precipitate amount, Tong Fachu again
Managing three times, drain for the last time, precipitate is acetone posterior lobe powder, adds the cold of 3 times of acetone posterior lobe powder amounts in acetone posterior lobe powder
Ether, is stirred at room temperature 1 hour, filters after standing 30 minutes, volatilization to the greatest extent ether, obtains ether posterior lobe powder, allows nothing in posterior lobe coarse powder
The component of drug effect separates out, and improves quality and the productivity of pituitrin；It is that 0.25% acetum is to lobus posterior hypophyseos by concentration
Powder extracts, and improves the efficiency of extraction；In extracting solution add ten thousand/ activated carbon, be heated rapidly to 100 DEG C of guarantors
Temperature 30 minutes, takes out and places 6～12 hours under the temperature conditions being positioned over less than-5 DEG C, take out coolant, first use paper pulp funnel
Sucking filtration, then through 30kD ultrafiltration membrance filter filter sucking filtration to clear and bright, obtain pituitary solution, by activated carbon to egg in extracting solution
White matter and impurity adsorb, then by paper pulp funnel sucking filtration, 30kD ultrafiltration membrance filter filter sucking filtration, improve pituitrin
Clarity and quality；Pituitary solution after the assay was approved is sub-packed in the stainless steel cask of cleaning, the plastics that bung is clean
Film wrapping seals, and labels, is placed in less than-5 DEG C and deposits, to extend the pituitrin shelf-life outside bucket.
The invention has the beneficial effects as follows:
The biological extraction method that the present invention provides produces medicinal pituitrin technique, by cold acetone and cold diethyl ether to posterior lobe
Powder processes, and the productivity of pituitrin is high；By activated carbon, protein in extracting solution and impurity are adsorbed, then pass through
Paper pulp funnel sucking filtration, 30kD ultrafiltration membrance filter filter sucking filtration, the clarity of pituitrin is high, quality better；The hypophysis prepared
Pituitrin is deposited below-5 DEG C, pituitrin long shelf-life.
Accompanying drawing explanation
Fig. 1 is the production technology figure that in the present invention, biological extraction method produces medicinal pituitrin technique.
Fig. 2 is the artwork that in the present invention, biological extraction method produces prepared by the extracting solution of medicinal pituitrin technique.
Detailed description of the invention
Seeing Fig. 1, Fig. 2, biological extraction method produces medicinal pituitrin technique, comprises the following steps:
1) acetone, separation are just taken off
Take out the pig cerebellum being soaked in acetone, count in being placed in dish, remove broken pig cerebellum, granulate pig cerebellum
It is separated quickly into frontal lobe and posterior lobe with tweezers, and posterior lobe is placed in acetone soln immersion.
2) prepared by posterior lobe coarse powder
From acetone soln, take out posterior lobe, and be filtered dry on funnel with gauze, after acetone volatilization to the greatest extent, put into drying baker
In, it being dried 5～7 days, dried dry posterior lobe is sent into reducing mechanism and is ground into posterior lobe powder, and posterior lobe powder crosses 40～60 mesh sieves, collects
Posterior lobe coarse powder is also weighed.
3) prepared by ether posterior lobe powder
In posterior lobe coarse powder, add the cold acetone of 3 times of posterior lobe coarse powder amounts, be stirred at room temperature 1.5 hours, stand 6～12 hours,
Pouring out supernatant, precipitate adds the cold acetone of 1 times of precipitate amount, same treatment three times again, drains for the last time, and precipitate is third
Ketone posterior lobe powder, adds the cold diethyl ether of 3 times of acetone posterior lobe powder amounts in acetone posterior lobe powder, is stirred at room temperature 1 hour, after standing 30 minutes
Filter, volatilization ether to the greatest extent, obtain ether posterior lobe powder.
4) prepared by Powdered Posterior Pituitary
Ether posterior lobe powder is put in drying baker, is dried 5～7 days, takes out, obtain hypophysis after moisture is reduced to below 6%
Posterior lobe powder, Powdered Posterior Pituitary sterilization, the sealing of clean film bundling, tagged state mark is placed the cryostat of less than 5 DEG C and is protected
Deposit.
5) prepared by extracting solution
Weighing Powdered Posterior Pituitary 3000g, once extract, the step once extracted is: add in Powdered Posterior Pituitary
Concentration is 0.25% acetum 150000mL, stirs, extracts 15 minutes under 60～65 DEG C of temperature conditionss, Powdered Posterior Pituitary
The cold place of extracting solution stands 6～12 hours, the cold filter of extracting solution buchner funnel after standing, obtains extracting solution and filtering residue for the first time,
Adding concentration in filtering residue is 0.25% acetum 60000mL, carries out second extraction, the time of second extraction, temperature and filtration
Mode with once extract identical, obtain second time extracting solution, extracting solution is mixed to get extracting solution with for the first time extracting solution for the second time.
6) deproteinization
In extracting solution add ten thousand/ activated carbon, be heated rapidly to 100 DEG C be incubated 30 minutes, take out be positioned over-5
Place 6～12 hours under temperature conditions below DEG C, take out coolant, first with paper pulp funnel sucking filtration, then through 30kD ultrafilter membrane mistake
Filter filter sucking filtration, to clear and bright, obtains pituitary solution, and pituitary solution is sampled inspection.
7) pack, store
Pituitary solution after the assay was approved is sub-packed in the stainless steel cask of cleaning, the plastic foil wrapping that bung is clean
Seal, label outside bucket, be placed in less than-5 DEG C and deposit.
The proportion of described cold acetone is 0.820～0.830, and the temperature of cold acetone is 15 DEG C.
Described pulverizer is pottery pulverizer or ball milling altar.
Described drying baker all uses dry lime as desiccant.
Embodiment 1
Take out the pig cerebellum being soaked in acetone, count in being placed in dish, remove broken pig cerebellum, granulate pig cerebellum
It is separated quickly into frontal lobe and posterior lobe with tweezers, and posterior lobe is placed in acetone soln immersion；Posterior lobe is taken out from acetone soln, and
It is filtered dry on funnel with gauze, after acetone volatilization to the greatest extent, puts into and use dry lime as in the drying baker of desiccant, be dried 5 days,
Dried dry posterior lobe is sent into ceramics flour and is broken into posterior lobe powder, and posterior lobe powder crosses 40 mesh sieves, collects posterior lobe coarse powder and weighs；Backward
The cold acetone that the proportion adding 3 times of posterior lobe coarse powder amounts in leaf coarse powder is 0.820, temperature is 15 DEG C, is stirred at room temperature 1.5 hours, quiet
Putting 6 hours, pour out supernatant, precipitate adds the cold acetone of 1 times of precipitate amount, same treatment three times again, drains for the last time, heavy
Shallow lake thing is acetone posterior lobe powder, adds the cold diethyl ether of 3 times of acetone posterior lobe powder amounts, be stirred at room temperature 1 hour in acetone posterior lobe powder, stands
Filter after 30 minutes, volatilization ether to the greatest extent, obtain ether posterior lobe powder；Ether posterior lobe powder is put in drying baker, is dried 5 days, treats moisture
Taking out after being reduced to less than 6%, obtain Powdered Posterior Pituitary, Powdered Posterior Pituitary sterilization, clean film bundling seal, and stick shape
State mark is placed the cryostat of less than 5 DEG C and is preserved；Weigh Powdered Posterior Pituitary 3000g, once extract, the step once extracted
For: adding concentration in Powdered Posterior Pituitary is 0.25% acetum 150000mL, stirs, extracts 15 under 60 DEG C of temperature conditionss
Minute, the cold place of extracting solution of Powdered Posterior Pituitary stands 6 hours, the cold filter of extracting solution buchner funnel after standing, obtains for the first time
Extracting solution and filtering residue, in filtering residue add concentration be 0.25% acetum 60000mL, carry out second extraction, second extraction time
Between, temperature and filter type with once extract identical, obtain second time extracting solution, extracting solution mixes with extracting solution for the first time for the second time
Conjunction obtains extracting solution；In extracting solution add ten thousand/ activated carbon, be heated rapidly to 100 DEG C be incubated 30 minutes, taking-up is put
It is placed under the temperature conditions of less than-5 DEG C placement 6 hours, takes out coolant, first with paper pulp funnel sucking filtration, then through 30kD ultrafilter membrane
Filter filter sucking filtration the most clear and bright, obtain pituitary solution, pituitary solution is sampled inspection；After the assay was approved hang down
Body posterior lobe solution is sub-packed in the stainless steel cask of cleaning, and the plastic foil wrapping that bung is clean seals, and labels, be placed in-5 outside bucket
Deposit below DEG C.
Embodiment 2
Take out the pig cerebellum being soaked in acetone, count in being placed in dish, remove broken pig cerebellum, granulate pig cerebellum
It is separated quickly into frontal lobe and posterior lobe with tweezers, and posterior lobe is placed in acetone soln immersion；Posterior lobe is taken out from acetone soln, and
It is filtered dry on funnel with gauze, after acetone volatilization to the greatest extent, puts into and use dry lime as in the drying baker of desiccant, be dried 7 days,
Dried dry posterior lobe is sent into ball milling altar and is ground into posterior lobe powder, and posterior lobe powder crosses 60 mesh sieves, collects posterior lobe coarse powder and weighs；To posterior lobe
The cold acetone that the proportion adding 3 times of posterior lobe coarse powder amounts in coarse powder is 0.830, temperature is 15 DEG C, is stirred at room temperature 1.5 hours, stands
12 hours, pouring out supernatant, precipitate adds the cold acetone of 1 times of precipitate amount, same treatment three times again, drains for the last time, heavy
Shallow lake thing is acetone posterior lobe powder, adds the cold diethyl ether of 3 times of acetone posterior lobe powder amounts, be stirred at room temperature 1 hour in acetone posterior lobe powder, stands
Filter after 30 minutes, volatilization ether to the greatest extent, obtain ether posterior lobe powder；Ether posterior lobe powder is put in drying baker, is dried 7 days, treats moisture
Taking out after being reduced to less than 6%, obtain Powdered Posterior Pituitary, Powdered Posterior Pituitary sterilization, clean film bundling seal, and stick shape
State mark is placed the cryostat of less than 5 DEG C and is preserved；Weigh Powdered Posterior Pituitary 3000g, once extract, the step once extracted
For: adding concentration in Powdered Posterior Pituitary is 0.25% acetum 150000mL, stirs, extracts 15 under 65 DEG C of temperature conditionss
Minute, the cold place of extracting solution of Powdered Posterior Pituitary stands 12 hours, the cold filter of extracting solution buchner funnel after standing, obtains for the first time
Extracting solution and filtering residue, in filtering residue add concentration be 0.25% acetum 60000mL, carry out second extraction, second extraction time
Between, temperature and filter type with once extract identical, obtain second time extracting solution, extracting solution mixes with extracting solution for the first time for the second time
Conjunction obtains extracting solution；In extracting solution add ten thousand/ activated carbon, be heated rapidly to 100 DEG C be incubated 30 minutes, taking-up is put
It is placed under the temperature conditions of less than-5 DEG C placement 12 hours, takes out coolant, first with paper pulp funnel sucking filtration, then through 30kD ultrafilter membrane
Filter filter sucking filtration the most clear and bright, obtain pituitary solution, pituitary solution is sampled inspection；After the assay was approved hang down
Body posterior lobe solution is sub-packed in the stainless steel cask of cleaning, and the plastic foil wrapping that bung is clean seals, and labels, be placed in-5 outside bucket
Deposit below DEG C.
The present invention is exemplarily described by technical solution of the present invention above in conjunction with accompanying drawing, it is clear that the present invention is specifically real
Now it is not subject to the restrictions described above, if the various unsubstantialities that the method design that have employed the present invention is carried out with technical scheme
Improve, or the most improved design by invention and technical scheme directly apply to other occasion, all at the protection model of the present invention
Within enclosing.
Claims (4)
1. biological extraction method produces medicinal pituitrin technique, it is characterised in that comprise the following steps:
1) acetone, separation are just taken off
Take out the pig cerebellum being soaked in acetone, count in being placed in dish, remove broken pig cerebellum, granulate pig cerebellum tweezer
Son is separated quickly into frontal lobe and posterior lobe, and posterior lobe is placed in acetone soln immersion；
2) prepared by posterior lobe coarse powder
From acetone soln, take out posterior lobe, and be filtered dry on funnel with gauze, after acetone volatilization to the greatest extent, put in drying baker, dry
Dry 5～7 days, dried dry posterior lobe was sent into reducing mechanism and is ground into posterior lobe powder, and posterior lobe powder crosses 40～60 mesh sieves, collected posterior lobe thick
Powder is also weighed；
3) prepared by ether posterior lobe powder
In posterior lobe coarse powder, add the cold acetone of 3 times of posterior lobe coarse powder amounts, be stirred at room temperature 1.5 hours, stand 6～12 hours, pour out
Supernatant, precipitate adds the cold acetone of 1 times of precipitate amount, same treatment three times again, drains for the last time, after precipitate is acetone
Ye Fen, adds the cold diethyl ether of 3 times of acetone posterior lobe powder amounts in acetone posterior lobe powder, is stirred at room temperature 1 hour, mistake after standing 30 minutes
Filter, volatilization ether to the greatest extent, obtain ether posterior lobe powder；
4) prepared by Powdered Posterior Pituitary
Ether posterior lobe powder is put in drying baker, is dried 5～7 days, takes out, obtain lobus posterior hypophyseos after moisture is reduced to below 6%
Powder, Powdered Posterior Pituitary sterilization, the sealing of clean film bundling, tagged state mark is placed the cryostat of less than 5 DEG C and is preserved；
5) prepared by extracting solution
Weighing Powdered Posterior Pituitary 3000g, once extract, the step once extracted is: add concentration in Powdered Posterior Pituitary
It is 0.25% acetum 150000mL, stirs under 60～65 DEG C of temperature conditionss, extract 15 minutes, the extraction of Powdered Posterior Pituitary
The cold place of liquid stands 6～12 hours, the cold filter of extracting solution buchner funnel after standing, obtains extracting solution and filtering residue, filtering residue for the first time
Middle addition concentration is 0.25% acetum 60000mL, carries out second extraction, the time of second extraction, temperature and filter type
Identical with once extracting, obtain second time extracting solution, extracting solution is mixed to get extracting solution with extracting solution for the first time for the second time；
6) deproteinization
In extracting solution add ten thousand/ activated carbon, be heated rapidly to 100 DEG C be incubated 30 minutes, take out be positioned over-5 DEG C with
Under temperature conditions under place 6～12 hours, take out coolant, first with paper pulp funnel sucking filtration, then filter through 30kD ultrafiltration membrance filter
Device sucking filtration, to clear and bright, obtains pituitary solution, and pituitary solution is sampled inspection；
7) pack, store
Pituitary solution after the assay was approved is sub-packed in the stainless steel cask of cleaning, and the plastic foil wrapping that bung is clean is close
Envelope, labels outside bucket, is placed in less than-5 DEG C and deposits.
Biological extraction method the most according to claim 1 produces medicinal pituitrin technique, it is characterised in that described cold third
The proportion of ketone is 0.820～0.830, and the temperature of cold acetone is 15 DEG C.
Biological extraction method the most according to claim 1 produces medicinal pituitrin technique, it is characterised in that described pulverizing
Machine is pottery pulverizer or ball milling altar.
Biological extraction method the most according to claim 1 produces medicinal pituitrin technique, it is characterised in that described dry
Case all uses dry lime as desiccant.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610481617.9A CN106117319A (en) | 2016-06-27 | 2016-06-27 | Biological extraction method produces medicinal pituitrin technique |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610481617.9A CN106117319A (en) | 2016-06-27 | 2016-06-27 | Biological extraction method produces medicinal pituitrin technique |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109608521A (en) * | 2018-12-12 | 2019-04-12 | 上海上药第生化药业有限公司 | A kind of pituitrin solution and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102166226A (en) * | 2011-04-19 | 2011-08-31 | 安徽宏业药业有限公司 | Production process of posterior pituitary solution |
| CN103087152A (en) * | 2013-01-17 | 2013-05-08 | 南京新百药业有限公司 | Extraction process of oxytocin solution |
| CN105362293A (en) * | 2015-11-16 | 2016-03-02 | 南京新百药业有限公司 | Production technology of posterior pituitary injection |
-
2016
- 2016-06-27 CN CN201610481617.9A patent/CN106117319A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102166226A (en) * | 2011-04-19 | 2011-08-31 | 安徽宏业药业有限公司 | Production process of posterior pituitary solution |
| CN103087152A (en) * | 2013-01-17 | 2013-05-08 | 南京新百药业有限公司 | Extraction process of oxytocin solution |
| CN105362293A (en) * | 2015-11-16 | 2016-03-02 | 南京新百药业有限公司 | Production technology of posterior pituitary injection |
Non-Patent Citations (2)
| Title |
|---|
| 李良铸等编著: "《最新生化药物制备技术》", 31 March 2001, 中国医药科技出版社 * |
| 王友同等编著: "《生化制品的技术开拓》", 31 March 1987, 中国食品出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109608521A (en) * | 2018-12-12 | 2019-04-12 | 上海上药第生化药业有限公司 | A kind of pituitrin solution and preparation method thereof |
| CN109608521B (en) * | 2018-12-12 | 2021-06-11 | 上海上药第一生化药业有限公司 | Posterior pituitrin solution and preparation method thereof |
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Application publication date: 20161116 |