CN106074644A - The cryodesiccated preservation method of Radix Notoginseng - Google Patents
The cryodesiccated preservation method of Radix Notoginseng Download PDFInfo
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- CN106074644A CN106074644A CN201610408818.6A CN201610408818A CN106074644A CN 106074644 A CN106074644 A CN 106074644A CN 201610408818 A CN201610408818 A CN 201610408818A CN 106074644 A CN106074644 A CN 106074644A
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- radix notoginseng
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F26—DRYING
- F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
- F26B5/00—Drying solid materials or objects by processes not involving the application of heat
- F26B5/04—Drying solid materials or objects by processes not involving the application of heat by evaporation or sublimation of moisture under reduced pressure, e.g. in a vacuum
- F26B5/06—Drying solid materials or objects by processes not involving the application of heat by evaporation or sublimation of moisture under reduced pressure, e.g. in a vacuum the process involving freezing
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
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Abstract
The invention discloses a kind of cryodesiccated preservation method of Radix Notoginseng, comprise the following steps: (1) pretreatment: the Radix Notoginseng of timely collecting is removed impurity removing, clean by sterile water wash, dry surface moisture；(2) prefreezing: the Radix Notoginseng that step (1) obtains is cooled to 30～40 DEG C in vacuum desiccator, prefreezing 20～25h；(3) vacuum lyophilization: Radix Notoginseng step (2) obtained, in vacuum freeze drier, is evacuated to 30 50Pa, at condenser temperature 50～60 DEG C, lyophilization 10 15h, takes out；(4) evacuation packaging.The method of the present invention utilizes Vacuum Freezing & Drying Technology to maintain quality and the feature of Radix Notoginseng, not only technical process is simple, with low cost, and farthest keep the nutritional labeling of Radix Notoginseng and original shape, color when reservation Radix Notoginseng is fresh, taste, and product rehydration is preferable, it is simple to long-term storage and transport.
Description
Technical field
The present invention relates to the cryodesiccated preservation method of Radix Notoginseng.
Background technology
Radix Notoginseng (Panax notoginseng) is araliaceae ginseng plant, and Radix Notoginseng has another name called Radix Notoginseng, Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae), is famous
The rare medicinal herbs of China and foreign countries.Li Shizhen (1518-1593 A.D.) reputation is " Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae) ".And claim " ginseng qi-tonifying first, Radix Notoginseng enriches blood first, taste with and merit also
Deng, therefore claim Panax pseudoginseng, for the most precious person of Chinese medicine." Radix Notoginseng health care medicated diet, integrating treatment and mend and cuisines, residence is cured the disease in drink
Food, kills two birds with one stone.It is used as medicine with root, root stock, is the important characteristic resources of the traditional rare Chinese medicine of China and Yunnan Province, is also to send out
Exhibition Chinese medicine and the important source material of natural drug industry.Rare Chinese medicine, raw with can hemostasia and dissipation blood stasis, reducing swelling and alleviating pain, be that Yunnan is white
Medicine main component, also can be used as medicine with the floral leaf of a plant, work as tea-drinking.Radix Notoginseng is in systems such as cardiovascular, cerebrovascular, nerve, immunity
There is many pharmacologically actives, also there is the protective effect to hepatic injury and the effect such as antitumor, antiinflammatory, improve human immunity
Power, increases human nutrition, building body, slow down aging.Radix Notoginseng industry has great importance in China's medical and health cause.
Conventional notoginseng drying method is hot-air seasoning, boulton process and vacuum freeze-drying method.Studies have found that
The pseudo-ginseng root slices finished product Determination of Content of Ginsenoside Rg_1 that vacuum lyophilization obtains is significantly higher than vacuum drying and hot air drying, sense organ
Characteristic scoring is also significantly greater than vacuum drying and hot air drying, and dried pseudo-ginseng root slices finished surface yellow-white, smooth nothing are split
Trace, light weight, flavour of a drug are dense, have good color and luster and outward appearance.
Summary of the invention
It is an object of the invention to overcome the deficiencies in the prior art, it is provided that a kind of cryodesiccated preservation method of Radix Notoginseng.
Technical scheme is summarized as follows:
A kind of cryodesiccated preservation method of Radix Notoginseng, comprises the following steps:
(1) pretreatment: the Radix Notoginseng of timely collecting is removed impurity removing, clean by sterile water wash, dry surface moisture；
(2) prefreezing: the Radix Notoginseng that step (1) obtains is cooled to-30～-40 DEG C, prefreezing 20 in vacuum desiccator
～25h；
(3) vacuum lyophilization: Radix Notoginseng step (2) obtained, in vacuum freeze drier, is evacuated to 30-
50Pa, at condenser temperature-50～-60 DEG C, lyophilization 10-15h, takes out；
(4) evacuation packaging.
Radix Notoginseng is wild Radix Notoginseng, plantation Radix Notoginseng or the Radix Notoginseng adventitious root of group training.
Preferred: the temperature of step (2) described prefreezing is-35 DEG C, the prefreezing time is 22h.
Preferred: step (3) be the Radix Notoginseng that step (2) is obtained in vacuum freeze drier, be evacuated to 40Pa,
Condenser temperature-55 DEG C, lyophilization 13h.
Advantages of the present invention:
The present invention utilizes Vacuum Freezing & Drying Technology to maintain quality and the feature of Radix Notoginseng, and not only technical process is simple, becomes
This is cheap, and farthest keeps the nutritional labeling of Radix Notoginseng and original shape, color when reservation Radix Notoginseng is fresh, taste, and produces
Product rehydration is preferable, it is simple to stores for a long time, transport and sells.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further illustrated.
Embodiment 1
A kind of cryodesiccated preservation method of Radix Notoginseng, comprises the following steps:
(1) pretreatment: the wild Radix Notoginseng of timely collecting is removed impurity removing, clean by sterile water wash, dry surface moisture；
(2) prefreezing: the Radix Notoginseng that step (1) obtains is cooled to-30 DEG C in vacuum desiccator, prefreezing 25h；
(3) vacuum lyophilization: Radix Notoginseng step (2) obtained, in vacuum freeze drier, is evacuated to 30Pa,
Condenser temperature-50 DEG C, lyophilization 15h, takes out；
(4) evacuation packaging.
Embodiment 2
A kind of cryodesiccated preservation method of Radix Notoginseng, comprises the following steps:
(1) pretreatment: (Radix Notoginseng being respectively as follows: wild Radix Notoginseng, plantation Radix Notoginseng and group training is indefinite by the Radix Notoginseng of timely collecting
Root) remove impurity removing, clean by sterile water wash, dry surface moisture；
(2) prefreezing: respectively three kinds of Radix Notoginseng that step (1) obtains are cooled to-35 DEG C in vacuum desiccator, prefreezing
22h；
(3) vacuum lyophilization: the three kinds of Radix Notoginseng respectively step (2) obtained in vacuum freeze drier, evacuation
To 40Pa, at condenser temperature-55 DEG C, lyophilization 13h, take out；
(4) evacuation packaging.
Wherein total saponin content refers to table 1.
Embodiment 3
A kind of cryodesiccated preservation method of Radix Notoginseng, comprises the following steps:
(1) pretreatment: the Radix Notoginseng adventitious root of the group training of timely collecting is removed impurity removing, clean by sterile water wash, dry
Surface moisture；
(2) prefreezing: the Radix Notoginseng that step (1) obtains is cooled to-40 DEG C in vacuum desiccator, prefreezing 20h；
(3) vacuum lyophilization: Radix Notoginseng step (2) obtained, in vacuum freeze drier, is evacuated to 50Pa,
Condenser temperature-60 DEG C, lyophilization 10h, takes out；
(4) evacuation packaging.
Embodiment 4
The HPLC quantitative analysis of saponin in Radix Notoginseng:
Experimental apparatus and reagent:
Agilent 1100 high performance liquid chromatograph, including online degasser and quaternary pump.Arasaponin R1, ginsenoside
Rg1、Re、Rb1、Rg2, Rd reference substance be purchased from Nat'l Pharmaceutical & Biological Products Control Institute, Chromatographic Pure Methanol, trifluoroacetic acid aqueous solution, ultrapure
Water.
Method:
1) chromatographic condition
Chromatographic column is kromasil C18(4.6mm × 250mm, 5 μm) post；Mobile phase A: 0.005% aqueous formic acid, B:
The acetonitrile solution of 0.005% formic acid.Gradient elution program: 0 → 35min, B:21%；35 → 36min, B:21% → 30%；36
→ 55min, B:30% → 40%；55 → 65min, B:40% → 85%；65→80min.Detection wavelength 200nm；Column temperature 25 DEG C；
Flow velocity 1.0mL min-1；Sampling volume is 10 μ L.
2) preparation of need testing solution
Precision weighs the laboratory sample (Radix Notoginseng of the present embodiment acquisition and fresh Radix Notoginseng) of about 0.2g drying and crushing and is placed in
In 100mL tool plug triangular flask, add 40mL methanol, 60 DEG C of water-bath 1h, be repeated once, while hot sucking filtration, collect filtrate.Filtrate is waved
It is dissolved in 10mL distilled water after Gan, with 20mL n-butanol extraction overnight, collects n-butanol layer, more rotated evaporation process (temperature 70
DEG C, rotating speed 50rpm) after, constant volume to 1mL.With 0.22 μm filtering with microporous membrane, obtain sample test sample.
3) preparation of saponin reference substance solution
Precision weighs the arasaponin R1 through more than drying under reduced pressure 12h, ginsenoside Rg1、Re、Rb1、Rg2, each 1mg of Rd,
Adding a small amount of methanol ultrasonic dissolution to be placed in 5mL volumetric flask, again with methanol is settled to scale, is made into saponin mixing reference substance deposit
Liquid.Reference substance storing solution is put in refrigerator with sealing after 0.22 μm filtering with microporous membrane and is saved backup.
Testing result is shown in Table 1:
Table 1
Experiment proves: the method for embodiment 1 measures Panax Notoginseng saponin R l, ginsenoside Rg l, people to the dried of wild Radix Notoginseng
Each to after wild notoginseng drying of ginseng saponin Re, ginsenoside Rb1, ginsenoside Rg2, ginsenoside Rd's content and embodiment 2
From content similar；
The method of embodiment 3 measures Panax Notoginseng saponin R l, ginsenoside Rg l, Radix Ginseng soap after drying to group training Radix Notoginseng adventitious root
Glycosides Re, ginsenoside Rb1, ginsenoside Rg2, ginsenoside Rd's content and embodiment 2 are dried to group training Radix Notoginseng adventitious root
Respective content is similar.
Show that compound components of panax notoginseng prepared by the method for the present invention does not changes by comparing, multiple after notoginseng drying
Aqueous is preferable, and outward appearance, color and luster after rehydration are similar to fresh Radix Notoginseng, and drug effect, quality do not change, and rehydration is preferable, makes to disappear
Expense person is willing to accept.
Claims (4)
1. the cryodesiccated preservation method of Radix Notoginseng, it is characterised in that comprise the following steps:
(1) pretreatment: the Radix Notoginseng of timely collecting is removed impurity removing, clean by sterile water wash, dry surface moisture；
(2) prefreezing: the Radix Notoginseng that step (1) obtains is cooled in vacuum desiccator-30～-40 DEG C, prefreezing 20～
25h；
(3) vacuum lyophilization: Radix Notoginseng step (2) obtained, in vacuum freeze drier, is evacuated to 30-50Pa,
Condenser temperature-50～-60 DEG C, lyophilization 10-15h, takes out；
(4) evacuation packaging.
A kind of cryodesiccated preservation method of Radix Notoginseng the most according to claim 1, is characterized in that described Radix Notoginseng is wild three
Seven, plantation Radix Notoginseng or the Radix Notoginseng adventitious root of group training.
A kind of cryodesiccated preservation method of Radix Notoginseng the most according to claim 1 and 2, is characterized in that: step (2) is described pre-
The temperature freezed is-35 DEG C, and the prefreezing time is 22h.
A kind of cryodesiccated preservation method of Radix Notoginseng the most according to claim 1, is characterized in that described step (3) is: will
The Radix Notoginseng that step (2) obtains, in vacuum freeze drier, is evacuated to 40Pa, at condenser temperature-55 DEG C, lyophilization 13h.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107830689A (en) * | 2017-10-31 | 2018-03-23 | 山东华玫生物科技有限公司 | A kind of rose industrialization freezes production system and technique |
| CN109045090A (en) * | 2018-10-26 | 2018-12-21 | 元谋利明脱水蔬菜有限责任公司 | A kind of notoginseng drying technique |
| CN110721211A (en) * | 2019-10-22 | 2020-01-24 | 昆明理工大学 | Preparation method of pseudo-ginseng formula granular decoction pieces |
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| CN101961361A (en) * | 2009-07-23 | 2011-02-02 | 云南特安呐制药股份有限公司 | Method for preparing active pseudo-ginseng |
| CN102961424A (en) * | 2012-11-23 | 2013-03-13 | 云南云科药业有限公司 | Panax notoginseng polysaccharide extract and preparation method, preparations and applications thereof |
| CN104721244A (en) * | 2015-03-17 | 2015-06-24 | 麻栗坡县童心三七农民专业合作社 | Pseudo-ginseng drying process |
| CN104887732A (en) * | 2015-05-19 | 2015-09-09 | 苏州大学 | Gynura divaricata freeze-dried powder for reducing blood glucose, and preparing method and applications thereof |
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2016
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101301321A (en) * | 2008-07-04 | 2008-11-12 | 云南特安呐制药股份有限公司 | Vacuum freeze-drying technique of stem and leaf of notoginseng |
| CN101961361A (en) * | 2009-07-23 | 2011-02-02 | 云南特安呐制药股份有限公司 | Method for preparing active pseudo-ginseng |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107830689A (en) * | 2017-10-31 | 2018-03-23 | 山东华玫生物科技有限公司 | A kind of rose industrialization freezes production system and technique |
| CN109045090A (en) * | 2018-10-26 | 2018-12-21 | 元谋利明脱水蔬菜有限责任公司 | A kind of notoginseng drying technique |
| CN110721211A (en) * | 2019-10-22 | 2020-01-24 | 昆明理工大学 | Preparation method of pseudo-ginseng formula granular decoction pieces |
| CN110721211B (en) * | 2019-10-22 | 2021-09-17 | 昆明理工大学 | Preparation method of pseudo-ginseng formula granules |
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