CN106069752A - Taking root and hardening off method of a kind of African Chrysanthemum plantlet in vitro - Google Patents
Taking root and hardening off method of a kind of African Chrysanthemum plantlet in vitro Download PDFInfo
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- CN106069752A CN106069752A CN201610416356.2A CN201610416356A CN106069752A CN 106069752 A CN106069752 A CN 106069752A CN 201610416356 A CN201610416356 A CN 201610416356A CN 106069752 A CN106069752 A CN 106069752A
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- 238000000338 in vitro Methods 0.000 title claims abstract description 71
- 241000723353 Chrysanthemum Species 0.000 title claims abstract description 69
- 235000007516 Chrysanthemum Nutrition 0.000 title claims abstract description 69
- 238000000034 method Methods 0.000 title claims abstract description 24
- 238000005286 illumination Methods 0.000 claims abstract description 14
- 239000012883 rooting culture medium Substances 0.000 claims abstract description 14
- 238000005728 strengthening Methods 0.000 claims abstract description 14
- 241000196324 Embryophyta Species 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 230000006698 induction Effects 0.000 claims abstract description 6
- 239000011159 matrix material Substances 0.000 claims abstract description 5
- 239000001963 growth medium Substances 0.000 claims description 12
- RMOGWMIKYWRTKW-UONOGXRCSA-N (S,S)-paclobutrazol Chemical compound C([C@@H]([C@@H](O)C(C)(C)C)N1N=CN=C1)C1=CC=C(Cl)C=C1 RMOGWMIKYWRTKW-UONOGXRCSA-N 0.000 claims description 9
- 239000010408 film Substances 0.000 claims description 8
- 239000011888 foil Substances 0.000 claims description 8
- 229920001817 Agar Polymers 0.000 claims description 6
- 235000007164 Oryza sativa Nutrition 0.000 claims description 6
- 239000005985 Paclobutrazol Substances 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 6
- 229930006000 Sucrose Natural products 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- 239000003415 peat Substances 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 235000009566 rice Nutrition 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- CGIDKJRJBMFXKV-UHFFFAOYSA-N 6-n'-benzylpurine-6,6-diamine Chemical compound N1=CN=C2N=CN=C2C1(N)NCC1=CC=CC=C1 CGIDKJRJBMFXKV-UHFFFAOYSA-N 0.000 claims description 5
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims description 5
- YGGXZTQSGNFKPJ-UHFFFAOYSA-N methyl 2-naphthalen-1-ylacetate Chemical compound C1=CC=C2C(CC(=O)OC)=CC=CC2=C1 YGGXZTQSGNFKPJ-UHFFFAOYSA-N 0.000 claims description 5
- 235000019362 perlite Nutrition 0.000 claims description 5
- 239000010451 perlite Substances 0.000 claims description 5
- 239000013039 cover film Substances 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 3
- 239000002609 medium Substances 0.000 claims description 2
- 239000007921 spray Substances 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 2
- 239000002956 ash Substances 0.000 claims 2
- 230000004083 survival effect Effects 0.000 abstract description 10
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 238000012423 maintenance Methods 0.000 abstract description 4
- 230000010496 root system development Effects 0.000 abstract description 3
- 238000012258 culturing Methods 0.000 abstract description 2
- 235000015097 nutrients Nutrition 0.000 abstract description 2
- 241000209094 Oryza Species 0.000 description 4
- 102000018997 Growth Hormone Human genes 0.000 description 3
- 108010051696 Growth Hormone Proteins 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 239000000122 growth hormone Substances 0.000 description 3
- 230000014759 maintenance of location Effects 0.000 description 3
- VGKONPUVOVVNSU-UHFFFAOYSA-N naphthalen-1-yl acetate Chemical compound C1=CC=C2C(OC(=O)C)=CC=CC2=C1 VGKONPUVOVVNSU-UHFFFAOYSA-N 0.000 description 3
- 230000001932 seasonal effect Effects 0.000 description 3
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 2
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000012090 tissue culture technique Methods 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 241000735332 Gerbera Species 0.000 description 1
- 240000000208 Gerbera jamesonii Species 0.000 description 1
- 206010044565 Tremor Diseases 0.000 description 1
- 150000003851 azoles Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 231100000033 toxigenic Toxicity 0.000 description 1
- 230000001551 toxigenic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
Taking root and hardening off method of a kind of African Chrysanthemum plantlet in vitro, the method is by being cut into simple bud by the plantlet in vitro of growing thickly of African Chrysanthemum, strengthening seedling and rooting culture medium carries out illumination cultivation, root induction, lid hardening is not opened 1～3 month in greenhouse or culturing room after taking root, progressively open wide bottleneck hardening afterwards, then heel in cultivation matrix.The strengthening seedling and rooting culture medium of the present invention had both been provided that the nutrient of abundance, can suitably delay again the speed of growth of African Chrysanthemum plantlet in vitro, shorten the maintenance management time on seedbed for the African Chrysanthemum plantlet in vitro, production at hot summer and severe winter uninterrupted African Chrysanthemum plantlet in vitro, it is greatly saved labour and the water power cost of seedbed management, simple to operate, convenient management, simultaneously, the African Chrysanthemum seedling robust plant that the present invention cultivates, root system development is good, heels in rear survival rate more than 99%, all can survive after seedling transplant planting.
Description
Technical field
The invention belongs to field of plant tissue culture technique, be specifically related to taking root and hardening side of a kind of African Chrysanthemum plantlet in vitro
Method.
Background technology
African Chrysanthemum (Gerbera jamesonii) is composite family Gerbera herbaceos perennial, has pattern and enriches, produces
The flower amount advantage such as height, viewing period length, is one of big cut-flower in the world five, is widely used in the aspects such as potted plant, Fresh Cutting flower.
With the development of flowers market, African Chrysanthemum production seedling amount increases day by day, utilizes tissue culture technique to carry out
The Fast-propagation of African Chrysanthemum seedling, has obtained developing faster.This technology can overcome that conventional means breeding seedling speed is slow, shadow
Ring new varieties popularization and seedling offspring can morph and cannot keep the defects such as maternal plant good characteristic.
Tissue culture technique is the important method of Fast-propagation African Chrysanthemum seedling, but, plantlet in vitro heel in be one from
Heterotrophism, to the process of autotrophy, there is also managerial skills and requires high and transplant the low problem of survival rate, more prominent, by season
Joint impact, when transplanting in summer and winter, the transplanting survival rate of African Chrysanthemum plantlet in vitro is substantially reduced.
In order to overcome summer high temperature or winter low temperature to cause the low defect of survival rate to the African Chrysanthemum seedling after transplanting, generally
Need to be located at seedbed in greenhouse, but, seedbed capacity is little, and running expense is high, and the toxigenic capacity of single seedling is substantially increased, and sometimes sets
When standby condition is bad, before hot summer and severe winter arrive, just stops the propagation of African Chrysanthemum plantlet in vitro, take root, to avoid in the hot summer and to tremble with fear
Winter carries out heeling in of plantlet in vitro, thus, about stops production about 3～4 months every year, and, utilize existing African Chrysanthemum plantlet in vitro
Take root and hardening off method, get rid of seasonal effect, African Chrysanthemum plantlet in vitro heel in survival rate about 90%.
Content of the invention
It is an object of the invention to provide taking root and hardening off method of a kind of African Chrysanthemum plantlet in vitro, the method can effectively shorten
The maintenance management time on seedbed for the plantlet in vitro, sustainable with cold winter during the broiling summer carry out African Chrysanthemum incessantly
The production of plantlet in vitro, is also greatly saved labor cost and the water power cost of seedbed management, simple to operate, convenient management, becomes
This is low, and seedling root system development is good, heels in rear survival rate of plant and can reach more than 99%, and restoration ecosystem is fast.
In order to achieve the above object, the present invention provides following technical scheme:
Taking root and hardening off method of a kind of African Chrysanthemum plantlet in vitro, comprises the steps:
1) plantlet in vitro of growing thickly of African Chrysanthemum is cut into simple bud, proceeds to, in the blake bottle equipped with strengthening seedling and rooting culture medium, cover
Bottle cap, carries out illumination cultivation, root induction, it is thus achieved that the African Chrysanthemum plantlet in vitro taken root；Wherein, in described strengthening seedling and rooting culture medium
Contain: 1/2MS improved culture medium, 6-benzyl aminoadenine, indolebutyric acid, methyl α-naphthyl acetate, paclobutrazol, sucrose and agar powder；
2) by step 1) in the African Chrysanthemum plantlet in vitro taken root, do not open 1～March of lid hardening, intensity of illumination 1500～
3000lux, temperature is 8～26 DEG C；
3) bottleneck of blake bottle reserves gap, continues hardening 3～4d, then opens wide bottleneck completely, adds 5～10mL water, then
Hardening 1～2d；
4) by step 3) in take out through opening wide the African Chrysanthemum plantlet in vitro after bottleneck hardening, the culture medium of removal base portion, by non-
Continent chrysanthemum plantlet in vitro is heeled in the cultivation matrix in seedbed, water spray, cover film, and heat and moisture preserving cultivates 7～10d；
5) film is opened, watering, keep seedbed to moisten, garden can be gone out after Plant technique 25-40d and transplant.
Preferably, step 1) described in strengthening seedling and rooting culture medium contains: 1/2MS improved culture medium, 6-benzyl amino gland is fast
Purine (6-BA) 0.05～0.1mg/L, indolebutyric acid (IBA) 0.1～1.0mg/L, methyl α-naphthyl acetate (NAA) 0.05～0.2mg/L, multiple-effect
Azoles (pp333) 0.05～0.2mg/L, sucrose 15～25g/L and agar powder 5.5～6.5g/L.
Further, described step 1) in, the cultivation temperature of illumination cultivation is 25 ± 2 DEG C, light application time is 12～14 hours/
My god, intensity of illumination is 1500～2500lux.
Preferably, step 2) in hardening when, temperature is 8～18 DEG C.
Further, step 3) in, bottleneck reserves gap, refers to open bottle cap, is put in bottle cap and reserves space on bottleneck, or
Person takes away bottle cap, covers bottleneck with plastic foil.
Further, step 4) in after cover film, cultivation temperature is 15～26 DEG C, and relative humidity is 75～85%, preferably
Cultivation temperature is 20～24 DEG C.
Further, step 4) in film be plastic foil.
Further, step 5) in, described transplanting medium is rice chaff ash, peat and perlitic mixture, wherein, rice chaff
Ash: perlite: the weight of peat ratio is for 1:4～6:5～8.
The present invention extends the retention time on strengthening seedling and rooting culture medium for the African Chrysanthemum plantlet in vitro, overcomes the training of African Chrysanthemum group
By the problem that seasonal effect is bigger in seedling production, utilize the present invention, before hot summer and season in severe winter arrive, be not required to stop to Africa
The propagation of chrysanthemum plantlet in vitro or culture of rootage, African Chrysanthemum plantlet in vitro can uninterruptedly be cultivated in Multiplying culture or root induction, it is thus achieved that take root
The follow-up continuation of insurance of plantlet in vitro is held on root media.
The present invention with the addition of various plants growth hormone in strong sprout with root media, such as 6-benzyl aminoadenine, Yin
Diindyl butyric acid, methyl α-naphthyl acetate and paclobutrazol, the effect adding 6-benzyl aminoadenine is to make African Chrysanthemum plantlet in vitro in the life of long period
Root keeps growth vigor in cultivating, it is to avoid decline；And the usual more application of paclobutrazol is in field, making crop downgrade, the present invention adds
Add paclobutrazol and can delay the speed of growth when culture of rootage for the African Chrysanthemum plantlet in vitro, there is the effect in strong sprout.
Work in coordination between the various plants growth hormone that the present invention adds, provide good life for African Chrysanthemum plantlet in vitro
Long environment, strong sprout of the present invention and root media be both provided that the nutrient of abundance, and can suitably delay again African Chrysanthemum plantlet in vitro
The speed of growth so that it is the growth time on strong sprout and root media extends, it is to avoid occur during hardening after taking root
Decline sign, shortens the hardening time on seedbed.The African Chrysanthemum plantlet in vitro of the present invention while taking root, growth more strong
Strong, seedling quality is improved, and improves the transplanting survival rate of African Chrysanthemum seedling further.
The present invention extends the retention time on strengthening seedling and rooting culture medium for the African Chrysanthemum plantlet in vitro, shortens plantlet in vitro at seedling
The hardening time on bed, not only can increase output, reduce cost, and seedling quality can be strengthened, shorten plantlet in vitro restoration ecosystem
Time, improve the transplanting survival rate that seedling is heeled in, positive effect is had to the plantlet in vitro productivity improving enterprise.
Compared with prior art, the method have the advantages that
1) strong sprout of the present invention and the various plants growth hormone of interpolation in root media are worked in coordination, and extend Africa
Retention time on strengthening seedling and rooting culture medium for the chrysanthemum plantlet in vitro, simultaneously, it is to avoid African Chrysanthemum plantlet in vitro occurs during hardening
The phenomenon of decline, can carry out heeling in of plantlet in vitro in hot summer or severe winter, overcome propagation or the culture of rootage of African Chrysanthemum plantlet in vitro
When suffered seasonal effect, reduce cost, increase output, the cost of labor of the seedbed management saving plantlet in vitro becomes with water power
This, also can improve seedbed utilization rate, saves and heels in space.
2) present invention extends growth time on strengthening seedling and rooting culture medium for the African Chrysanthemum plantlet in vitro, during this, and group training
Seedling also grows slow, constantly sturdy, more adapts to the environment in the external world, through progressively hardening, improves the adaptability of seedling, then heels in
In seedbed, seedling recovers soon on seedbed, robust plant, heels in survival rate high, can reach more than 99%.It is also greatly shortened and return
The seedling time, it is easier to nurturing staff, improving seedling quality, seedling root system development is good, is also beneficial to follow-up African Chrysanthemum seedling fixed
Plant.
Detailed description of the invention
Below in conjunction with specific embodiment, the invention will be further described.
The 1/2MS improved culture medium formula of the present invention is as shown in table 1 below, and mg/L represents in every liter of culture medium containing each composition
Milligram number.
Table 1
Embodiment 1 carries out taking root and hardening of African Chrysanthemum plantlet in vitro in summer, mainly comprises the steps that
1) mid or late July, the plantlet in vitro of growing thickly deriving from Multiplying culture African Chrysanthemum is cut into simple bud, proceeds to equipped with strong sprout raw
In the blake bottle of root culture medium, covering bottle cap, carrying out illumination cultivation, root induction, temperature is 25 ± 2 DEG C, intensity of illumination is
About 2500lux, culture of rootage 25～30d, 100% plantlet in vitro is taken root, it is thus achieved that the African Chrysanthemum plantlet in vitro taken root；
Wherein, described strengthening seedling and rooting culture medium is: 1/2MS+6-BA 0.1mg/L+IBA0.3mg/L+NAA 0.1mg/L
+ pp333 0.1mg/L+ sucrose 20g/L+ agar powder 6.0g/L；
2) by step 1) in the African Chrysanthemum plantlet in vitro taken root do not open lid, be placed in culturing room and keep 2 months, intensity of illumination
1500～2000lux, temperature is 24 ± 2 DEG C；
3) mid or late October, open the bottle cap of blake bottle, bottle cap is put on bottleneck, leave about 1/3 space, keep logical
Wind, hardening 3～4d, then open wide bottleneck completely, add 5～10mL water, hardening 1d；
4) take out step 3) in African Chrysanthemum plantlet in vitro after hardening, clean the culture medium of African Chrysanthemum plantlet in vitro base portion, by it
Heeling in seedbed, the cultivation matrix in seedbed is rice chaff ash: perlite: peat, by weight the ratio mixing for 1:4:5, waters in time
Water, covers the seedbed heeling in African Chrysanthemum plantlet in vitro, keeps temperature 18～26 DEG C, humidity about 80%, maintenance with plastic foil
10d；
5) film is opened, watering, keep seedbed to moisten, manage according to a conventional method afterwards, heeling in into of African Chrysanthemum plantlet in vitro
Motility rate reaches 99%, and seedling is sturdy, well-grown, and about 25d can go out garden field planting.
Embodiment 2 carries out taking root and hardening of African Chrysanthemum plantlet in vitro in the winter time, mainly comprises the steps that
1) mid-November, the plantlet in vitro of growing thickly deriving from Multiplying culture African Chrysanthemum is cut into simple bud, proceeds to equipped with strong sprout raw
In the blake bottle of root culture medium, covering bottle cap, carrying out illumination cultivation, root induction, temperature is 25 ± 2 DEG C, intensity of illumination is
About 2500lux, culture of rootage 25～30d, 100% plantlet in vitro is taken root, it is thus achieved that the African Chrysanthemum plantlet in vitro taken root；
Wherein, described strengthening seedling and rooting culture medium is: 1/2MS+6-BA 0.05mg/L+IBA0.5mg/L+NAA
0.05mg/L+pp333 0.1mg/L+ sucrose 20g/L+ agar powder 6.0g/L；
2) by step 1) in the African Chrysanthemum plantlet in vitro taken root do not open lid and be placed in greenhouse hardening 2.5 months, intensity of illumination
About 3000lux, temperature is 10-26 DEG C；
3) at the beginning of 3 months next year, open the bottle cap of blake bottle, cover bottleneck with plastic foil, stay ventilating opening, hardening 3～4d, throw off
Plastic foil, adds 5～10mL distilled water, hardening 1d；
4) throw off plastic foil the 2nd day, takes out plantlet in vitro with tweezers, cleans the culture medium of its base portion, is heeled in seedling
Bed, the cultivation matrix in seedbed is rice chaff ash: perlite: peat, by weight the ratio mixing for 1:6:8, waters, in time with moulding
Material film covers the seedbed heeling in African Chrysanthemum plantlet in vitro, keeps temperature 18～26 DEG C, humidity more than 80%, maintenance 10d；
5) film is opened, watering, keep seedbed to moisten, manage according to a conventional method afterwards, after cultivating 25d, heel in survival rate
More than 99%, seedling is sturdy, well-grown, can go out garden.
Claims (9)
1. the taking root and a hardening off method of African Chrysanthemum plantlet in vitro, comprises the steps:
1) plantlet in vitro of growing thickly of African Chrysanthemum is cut into simple bud, proceeds to, in the blake bottle equipped with strengthening seedling and rooting culture medium, cover bottle cap,
Carry out illumination cultivation, root induction, it is thus achieved that the African Chrysanthemum plantlet in vitro taken root；Wherein, described strengthening seedling and rooting culture medium contains:
1/2MS improved culture medium, 6-benzyl aminoadenine, indolebutyric acid, methyl α-naphthyl acetate, paclobutrazol, sucrose and agar powder；
2) by step 1) in the African Chrysanthemum plantlet in vitro taken root, do not open 1～March of lid hardening, intensity of illumination 1500～3000lux,
Temperature is 8～26 DEG C；
3) bottleneck of blake bottle is reserved gap, continue hardening 3～4d, then open wide bottleneck completely, add 5～10mL water, then refine
Seedling 1～2d；
4) by step 3) in African Chrysanthemum plantlet in vitro after hardening of abroaching take out, remove base portion culture medium, African Chrysanthemum group trained
Seedling is heeled in the cultivation matrix in seedbed, water spray, cover film, and heat and moisture preserving cultivates 7～10d；
5) film is opened, watering, keep seedbed to moisten, garden can be gone out after Plant technique 25～40d and transplant.
2. the taking root and hardening off method of African Chrysanthemum plantlet in vitro according to claim 1, it is characterised in that step 1) described in
Strengthening seedling and rooting culture medium contains: 1/2MS improved culture medium, 6-benzyl aminoadenine 0.05～0.1mg/L, indolebutyric acid 0.1
～1.0mg/L, methyl α-naphthyl acetate 0.05～0.2mg/L, paclobutrazol 0.05～0.2mg/L, sucrose 15～25g/L and agar powder 5.5～
6.5g/L。
3. the taking root and hardening off method of African Chrysanthemum plantlet in vitro according to claim 1, it is characterised in that step 1) in, light
Being 25 ± 2 DEG C according to the cultivation temperature cultivated, light application time is 12～14 hours/day, and intensity of illumination is 1500～2500lux.
4. the taking root and hardening off method of African Chrysanthemum plantlet in vitro according to claim 1, it is characterised in that step 2) in hardening
When, temperature is 8～18 DEG C.
5. the taking root and hardening off method of African Chrysanthemum plantlet in vitro according to claim 1, it is characterised in that step 3) in, institute
The bottleneck stated reserves gap, refers to open bottle cap, is put in bottle cap on bottleneck and reserves space, or bottle cap of taking away, uses plastic foil
Cover bottleneck.
6. the taking root and hardening off method of African Chrysanthemum plantlet in vitro according to claim 1, it is characterised in that step 4) in cover
After film, cultivation temperature is 15～26 DEG C, and relative humidity is 75～85%.
7. the taking root and hardening off method of the African Chrysanthemum plantlet in vitro according to claim 1 or 6, it is characterised in that step 4) in
After cover film, cultivation temperature is 20～24 DEG C, and relative humidity is 75～85%.
8. the taking root and hardening off method of the African Chrysanthemum plantlet in vitro according to claim 6 or 7, it is characterised in that step 4) in,
Described film is plastic foil.
9. the taking root and hardening off method of African Chrysanthemum plantlet in vitro according to claim 1, it is characterised in that step 5) in, institute
State and transplant the mixture that required transplanting medium is rice chaff ash, perlite and peat, wherein, rice chaff ash: perlite: the weight of peat
Amount ratio is 1:4～6:5～8.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109964816A (en) * | 2019-04-15 | 2019-07-05 | 云南省农业科学院花卉研究所 | A kind of method for culturing seedlings of African Chrysanthemum monoploid transplanted seedling |
| CN112868526A (en) * | 2021-01-26 | 2021-06-01 | 淮阴工学院 | African daisy tissue culture seedling raising liquid rooting culture medium, plant fixer and rooting method |
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