CN101496496A - Tissue culture and rapid propagation method for Gerbera jamesonii Bolus - Google Patents
Tissue culture and rapid propagation method for Gerbera jamesonii Bolus Download PDFInfo
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- CN101496496A CN101496496A CNA2008100146046A CN200810014604A CN101496496A CN 101496496 A CN101496496 A CN 101496496A CN A2008100146046 A CNA2008100146046 A CN A2008100146046A CN 200810014604 A CN200810014604 A CN 200810014604A CN 101496496 A CN101496496 A CN 101496496A
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- flameray gerbera
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Abstract
The invention discloses a tissue culture and quick propagation method for flameray gerbera, and in particular relates to a flameray gerbera two-step seedling raising method. A leaf of a test-tube seedling is taken as an explant to directly induce an indefinite bud and carry out the rootage culture; and the whole process of the flameray gerbera tissue culture seedling is completed in two steps. The method which is used to produce the flameray gerbera germchit not only solves the problem that the flameray gerbera black pistil variety has difficulty in the indefinite bud induction and a low induction rate, but also breaks down the boundary between the flameray gerbera green pistil variety and the flameray gerbera black pistil variety, and realizes above 98 percent indefinite bud high-efficiency induction for the green pistil variety and above 90 percent indefinite high-efficiency induction for the black pistil variety through one culture medium. In addition, the method simplifies the tissue culture process. The method not only reduces the tissue culture period from originally 7 months to 4 months, but also reduces a shifting procedure. Therefore, the tissue culture cost is reduced by 1/3.
Description
Technical field
The present invention relates to flowers culture technique field, especially a kind of tissue culture and rapid propagation method for Gerbera jamesonii Bolus---flameray gerbera " two go on foot into the seedling method ".
Background technology
Flameray gerbera (Gerbera jamesonii bolus) has another name called African daisy, sun chrysanthemum etc., is composite family Gerbera perennial evergreen perennial root herbage flower.The flameray gerbera ornamental value is higher, and its flower is very large, and faceplate diameter can reach 8-10cm, and spray is tall and straight, and the extremely favor of consumption has become one of flower variety the most salable in the flowers market, the world at present because of the meaning that " helping man, the cause prosperity " arranged.Flameray gerbera multiplication technique backwardness is the main cause that causes China's flameray gerbera slower development, flameray gerbera is a cross-pollinatd plant, selfing is infertile, the very low and short sprouting power of very easily losing of seed longevity of ripening rate under the natural conditions, its seed progeny breeds and loses original kind of property easily simultaneously, morph, cause that bennet attenuates, deliquescing, flower type diminish etc.Division propagation can keep original kind of property feature, do not morph, but seedling is infected by sick worm easily in reproductive process, and growth rate is slow, is difficult to satisfy vigorous market to producing the active demand with seedling.Therefore foundation flameray gerbera seedling production technology system rapidly and efficiently is of great immediate significance to the industrialized development of China's flameray gerbera flower industry.
Flameray gerbera is in the stage that develops rapidly on International Flower market, needs a large amount of breeding seedlings in the production, and China is in the popularization stage, and supply falls short of demand for the breeding seedling, and seed and seedling are all from external introduction, and cost is very high.Many research institutes (institute) have obtained regeneration plant by the method for group training, but " three go on foot into seedling " methods that adopt routine more, promptly from outside grow body and induce callus, go out indefinite bud by callus induction again, carry out adventitious bud rooting then and induce three steps, the cycle of its breeding seedling is more than 7 months, and is expensive because of organizing the higher flameray gerbera seedling price that causes of training cost.Flameray gerbera is wide in variety in addition, germplasm type complexity, although with holder, petal, tender leaf, stem apex, base of leaf is that the initial outer body cultivation prescription of growing is announced, but the result of study difference between different cultivars is big, particularly utilize the plant regeneration that is difficult to realize green stamen of flameray gerbera and black stamen kind with a kind of culture medium prescription, for seedling manufacturing enterprise, still need to be optimized tissue culture technology.
Summary of the invention
The purpose of this invention is to provide a kind of at the green stamen kind of different flameray gerbera and black stamen kind can be shared the plant regeneration culture medium prescription, simplify the group training program of flameray gerbera, reduction group training cost.
In order to achieve the above object, the technical solution used in the present invention may further comprise the steps:
(1) on superclean bench, grows body outside the conduct of clip flameray gerbera test-tube plantlet blade;
(2) the described outer body of growing is become the square of 2-6mm along the crosscut of vertical centering control arteries and veins direction, blade back is inoculated in the inducing culture down, and every bottle graft kind is counted the 10-25 strain, and described inducing culture is the MS medium that adds 6-BA 3.0-6.0mg/L, IAA 0.1-0.4mg/L;
(3) will be seeded in outer in the blake bottle and grow body and carry out 3 days dark place reasons earlier, be that 800-2000Lux, light application time 12-14h/d, temperature are to place under 20-28 ℃ the condition to cultivate under the green filter coating promptly to obtain the indefinite bud of inducing in 50-65 days in intensity of illumination then;
(4) described indefinite bud of inducing is inoculated in the root media, every bottle graft kind number is the 10-20 strain, in intensity of illumination is that 800-2000Lux, light application time 12-14h/d, temperature are to cultivate under 20-28 ℃ the condition can take root in 60-70 days, and described root media is the MS medium that is added with IAA 0.1-0.3mg/L.
Tissue culture and rapid propagation method for Gerbera jamesonii Bolus provided by the invention, not only solved the black stamen kind adventitious bud inducing problem of difficult of flameray gerbera, and broken the boundary of green stamen kind of flameray gerbera and black stamen kind, can realize that the indefinite bud of two strains efficiently induce by a kind of medium; The present invention is by finishing flameray gerbera tissue cultivating seedling production overall process with the test-tube plantlet sheet for growing direct evoking adventive bud of body and two steps of culture of rootage outward in addition, simplified group training program, not only organizing the training cycle shortened to 4 months by original 7 months, and reduced switching process one time, group training cost savings 1/3rd.
Embodiment
Describe the present invention below by specific embodiment:
Embodiment 1
(1) on superclean bench, grows body outside the conduct of clip flameray gerbera test-tube plantlet blade;
(2) will grow body outward along vertical centering control arteries and veins direction crosscut 3 cuttves, and cut off vein, and be cut into the square of 2mm, blade back is inoculated in the MS medium that is added with 6-BA3.0mg/L, IAA0.1mg/L down, and every bottle graft kind number is 25 strains;
(3) the outer body of growing that will be seeded in this blake bottle carries out 3 days dark place reasons earlier, be that 800Lux, light application time 14h/d, temperature are to place under the green filter coating under 28 ℃ the condition to cultivate 65 days in intensity of illumination then, the length that obtains inducing is the indefinite bud of 4cm, the inductivity of its medium green stamen kind is 99.8%, and the inductivity of black stamen kind is 91%;
(4) indefinite bud that obtains is inoculated in the MS medium that is added with IAA 0.3mg/L, every bottle graft kind number is 10 strains, in intensity of illumination is that 1500Lux, light application time 12h/d, temperature are to cultivate 60 days under 24 ℃ the condition, obtain the root that length is 7cm, rooting rate is 100%, and each plant on average takes root 8, the shank shape that is tubbiness, plant is tall and straight, and average height is at 12cm, and blade is a light green.
All additional in the above-described MS medium have 30% sucrose, 0.6% agar and the caseinhydrolysate of 300mg/L.
Embodiment 2
(1) on superclean bench, grows body outside the conduct of clip flameray gerbera test-tube plantlet blade;
(2) will grow body outward along vertical centering control arteries and veins direction crosscut 3 cuttves, and cut off vein, and be cut into the square of 4mm, blade back is inoculated in the MS medium that is added with 6-BA 5.0mg/L, IAA 0.3mg/L down, and every bottle graft kind number is 10 strains;
(3) the outer body of growing that will be seeded in this blake bottle carries out 3 days dark place reasons earlier, be that 1500Lux, light application time 13h/d, temperature are to place under the green filter coating under 25 ℃ the condition to cultivate 50 days in intensity of illumination then, the length that obtains inducing is the indefinite bud of 8cm, the inductivity of its medium green stamen kind is 100%, and the inductivity of black stamen kind is 97%;
(4) indefinite bud that obtains is inoculated in the MS medium that is added with IAA0.2mg/L, every bottle graft kind number is 15 strains, in intensity of illumination is that 800Lux, light application time 14h/d, temperature are to cultivate 65 days under 28 ℃ the condition, obtain the root that length is 5cm, rooting rate is 100%, and each plant on average takes root 6, the shank shape that is tubbiness, plant is tall and straight, and average height is at 10cm, and blade is a light green.
All additional in the above-described MS medium have 30% sucrose, 0.6% agar and the caseinhydrolysate of 300mg/L.
Embodiment 3
(1) on superclean bench, grows body outside the conduct of clip flameray gerbera test-tube plantlet blade;
(2) will grow body outward along vertical centering control arteries and veins direction crosscut 3 cuttves, and cut off vein, and be cut into the square of 6mm, blade back is inoculated in the MS medium that is added with 6-BA6.0mg/L, IAA0.4mg/L down, and every bottle graft kind number is 15 strains;
(3) the outer body of growing that will be seeded in this blake bottle carries out 3 days dark place reasons earlier, be that 2000Lux, light application time 12h/d, temperature are to place under the green filter coating under 20 ℃ the condition to cultivate 50 days in intensity of illumination then, the length that obtains inducing is the indefinite bud of 7cm, the inductivity of its medium green stamen kind is 100%, and the inductivity of black stamen kind is 94%;
(4) indefinite bud that obtains is inoculated in the MS medium that is added with IAA0.1mg/L, every bottle graft kind number is 20 strains, in intensity of illumination is that 2000Lux, light application time 14h/d, temperature are to cultivate 70 days under 26 ℃ the condition, obtain the root that length is 4cm, rooting rate is 100%, and each plant on average takes root 4, the shank shape that is tubbiness, plant is tall and straight, and average height is at 13cm, and blade is a light green.
All additional in the above-described MS medium have 30% sucrose, 0.6% agar and the caseinhydrolysate of 300mg/L.
In sum, method of operating of the present invention is simple, has not only simplified group training program, and has shortened tissue cultivating seedling breeding cycle greatly, the industrialization of flameray gerbera seedling is produced be of great immediate significance.Utilize this invention production flameray gerbera seedling, not only broken the boundary of black stamen kind of flameray gerbera and green stamen kind, and solved the black low difficult problem of stamen kind adventitious bud induction frequency of flameray gerbera in the production; In addition, utilize this method production flameray gerbera seedling, can keep former kind genetic stability preferably, even many generation breedings also are difficult for morphing, because of its regeneration plant of inducing is that cell without dedifferentiation forms, the cell somaclonal variation is little, can keep the good characteristic of acceptor plant preferably.
Claims (1)
1, a kind of tissue culture and rapid propagation method for Gerbera jamesonii Bolus is characterized in that, may further comprise the steps:
(1) on superclean bench, grows body outside the conduct of clip flameray gerbera test-tube plantlet blade;
(2) the described outer body of growing is become the square of 2-6mm along the crosscut of vertical centering control arteries and veins direction, blade back is inoculated in the inducing culture down, and every bottle graft kind is counted the 10-25 strain, and described inducing culture is the MS medium that adds 6-BA3.0-6.0mg/L, IAA0.1-0.4mg/L;
(3) will be seeded in outer in the blake bottle and grow body and carry out 3 days dark place reasons earlier, be that 800-2000Lux, light application time 12-14h/d, temperature are to place under 20-28 ℃ the condition to cultivate under the green filter coating promptly to obtain the indefinite bud of inducing in 50-65 days in intensity of illumination then;
(4) described indefinite bud of inducing is inoculated in the root media, every bottle graft kind number is the 10-20 strain, in intensity of illumination is that 800-2000Lx, light application time 12-14h/d, temperature are to cultivate under 20-28 ℃ the condition can take root in 60-70 days, and described root media is the MS medium that is added with IAA0.1-0.4mg/L.
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| CN2008100146046A CN101496496B (en) | 2008-02-02 | 2008-02-02 | Tissue culture and rapid propagation method for Gerbera jamesonii Bolus |
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| CN2008100146046A CN101496496B (en) | 2008-02-02 | 2008-02-02 | Tissue culture and rapid propagation method for Gerbera jamesonii Bolus |
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| CN101496496A true CN101496496A (en) | 2009-08-05 |
| CN101496496B CN101496496B (en) | 2012-02-08 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102415337A (en) * | 2011-09-13 | 2012-04-18 | 沈阳农业大学 | Breeding method of gerbera hybrida tissue culture plug seedlings |
| CN104719161A (en) * | 2015-03-23 | 2015-06-24 | 上海市农业科学院 | Method for obtaining African daisy regeneration plant through inducing somatic embryo |
| CN104737912A (en) * | 2015-03-31 | 2015-07-01 | 临沂大学 | Tissue culture and rapid propagation culture medium for gerbera jamesonii and culture method thereof |
| CN105660409A (en) * | 2016-02-26 | 2016-06-15 | 邓珂 | Huai-pearl chrysanthemum tissue culture method |
| CN106069752A (en) * | 2016-06-15 | 2016-11-09 | 上海市农业科学院 | Taking root and hardening off method of a kind of African Chrysanthemum plantlet in vitro |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1653879A (en) * | 2004-12-02 | 2005-08-17 | 广东省珠海市园艺研究所 | A flower cutting and seed planting rapid breeding method for flameray gerbera |
-
2008
- 2008-02-02 CN CN2008100146046A patent/CN101496496B/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102415337A (en) * | 2011-09-13 | 2012-04-18 | 沈阳农业大学 | Breeding method of gerbera hybrida tissue culture plug seedlings |
| CN104719161A (en) * | 2015-03-23 | 2015-06-24 | 上海市农业科学院 | Method for obtaining African daisy regeneration plant through inducing somatic embryo |
| CN104737912A (en) * | 2015-03-31 | 2015-07-01 | 临沂大学 | Tissue culture and rapid propagation culture medium for gerbera jamesonii and culture method thereof |
| CN105660409A (en) * | 2016-02-26 | 2016-06-15 | 邓珂 | Huai-pearl chrysanthemum tissue culture method |
| CN106069752A (en) * | 2016-06-15 | 2016-11-09 | 上海市农业科学院 | Taking root and hardening off method of a kind of African Chrysanthemum plantlet in vitro |
| CN106069752B (en) * | 2016-06-15 | 2018-05-08 | 上海市农业科学院 | A kind of African Chrysanthemum tissue-cultured seedling take root and hardening off method |
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| CN101496496B (en) | 2012-02-08 |
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