CN105660409A - Huai-pearl chrysanthemum tissue culture method - Google Patents
Huai-pearl chrysanthemum tissue culture method Download PDFInfo
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- CN105660409A CN105660409A CN201610106436.8A CN201610106436A CN105660409A CN 105660409 A CN105660409 A CN 105660409A CN 201610106436 A CN201610106436 A CN 201610106436A CN 105660409 A CN105660409 A CN 105660409A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention discloses a Huai-pearl chrysanthemum tissue culture method, relating to the technical field of tissue culture. The michelia alba tissue culture method disclosed by the embodiment comprises the following steps: selecting tender and fresh Huai-pearl chrysanthemum as an explant, disinfecting by using alcohol, inoculating to a bud induction culture medium, a rooting culture medium and a strong seedling culture medium in sequence, and finally culturing by seedling hardening. By adopting all the culture media and the method, the breeding period of the Huai-pearl chrysanthemum can be shortened to a greater degree, obtained seedlings are strong in growth, better in growth vigor, and relatively consistent in inheritable character, and the Huai-pearl chrysanthemumis can be bred without season limitation, and can be popularized broadly.
Description
Technical field
The present invention relates to technical field of tissue culture, especially a kind of method cherishing Cornmarigold tissue culture.
Background technology
Bosom Cornmarigold is the Huai Chrysanthemi kind that Wen County Institute of agricultural sciences employing systematic breeding method is cultivated, per nnial herb, and stem band purple is without rib, uprightly, grow thickly, high 85-110 centimetre, branch is less, and whole body is close by white-colored hairs, base minister's lignifying, single leaf alternate, ovum shape lanceolar, blade greyish-green, leaf splits relatively dark, and leaf length 4.9 centimetres is wide 3.3 centimetres, handle length 1.6 centimetres, head inflorescence, top is raw or armpit is raw. Flower is in spheroid, and diameter 2.5 centimetres, inflorescence peripheral ligulate flower number layer arrangement in many colyliforms, turns pink after white at the beginning of color and luster, turn red through frost, and centre is yellow tubiform floret, mid or late October at florescence. Bosom Cornmarigold " look, perfume (or spice), taste, shape " claims four absolutely, is used as medicine with flower bud or makes tea, Long Drinks, clearing heat and detoxicating, nourishes the liver to improve visual acuity, profit blood vessels; After bubbling open, delicate fragrance is assailed the nostrils, and is the treasure of house, tourism, present. Bosom Cornmarigold cold nature, micro-sweet, returns lung, Liver Channel, and purine-containing, choline, little Su alkali, amino acid etc. are multiple to human body beneficiating ingredient.
At present, bosom Cornmarigold seedling is mainly bred by traditional offshoot, causes reproduction speed slow, and bosom Cornmarigold growing way is bad, and axillary seedling is irregular, and tissue culture can realize the quick breeding cherishing Cornmarigold, and the domestic research to bosom Cornmarigold is less.
Summary of the invention
It is an object of the invention to provide a kind of method cherishing Cornmarigold tissue culture, this kind of bosom Cornmarigold tissue culture method can provide a kind of new propagation method.
In order to solve the problem, the technical solution used in the present invention is:
The method of this kind of bosom Cornmarigold tissue culture comprises the following steps:
A, choose children tender fresh bosom pearl chrysanthemum as explant, with the alcohol disinfecting of 75%, then rinse 3 times～4 times with sterile distilled water;
B, it is seeded to bud inducement substratum, natural lighting, cultivates 6 days～10 days to growing sprouting;
C, proceed to root media, natural lighting, cultivate 13 days～15 days to the root growing 1 centimetre～3 centimetres;
D, proceeding to strong seedling culture base, natural lighting, cultivate 10 days～15 days, grow tall 3 centimetres～4 centimetres to seedling, hardening cultivates 15 days～20 days;
Wherein, bud inducement substratum is taking 2/3MS substratum as minimum medium, adds 50 grams per liter～70 grams per liter 6-benzyl aminoadenine and 10 grams per liter～25 grams per liter indolylacetic acids;
Root media is taking MS substratum as minimum medium, adds 15 grams per liter～30 grams per liter Plant hormones regulators,gibberellins, 60 grams per liter～100 grams per liter glycine and 30 grams per liter～50 grams per liter peptones;
Strong seedling culture base is taking 1/2MS substratum as minimum medium, adds 8 grams per liter～20 grams per liter naphthylacetic acids, 5 grams per liter～10 grams per liter indolylacetic acids, 3 grams per liter～10 grams per liter organoselenium and 10 grams per liter～20 grams per liter indolebutyric acids.
In technique scheme, technical scheme can also be more specifically: organoselenium is selenium for egg amido acetic acid or selenium for cystamine hydrochloride.
Further, temperature when bud inducement is cultivated is room temperature; During root culture, temperature is 22 DEG C～26 DEG C; Temperature during strong seedling culture is room temperature.
MS medium component is: saltpetre 1.9 grams per liter, ammonium nitrate 1.65 grams per liter, potassium primary phosphate 170 mg/litre, magnesium sulfate 370 mg/litre, calcium chloride 440 mg/litre, potassiumiodide 0.83 mg/litre, boric acid 6.2 mg/litre, manganous sulfate 22.3 mg/litre, zinc sulfate 8.6 mg/litre, Sodium orthomolybdate 0.25 mg/litre, copper sulfate 0.25 mg/litre, cobalt chloride 0.025 mg/litre, disodium ethylene diamine tetraacetate 37.25 mg/litre, ferrous sulfate 27.85 mg/litre, inositol 100 mg/litre, glycine 2 mg/litre, vitamin 0.1 mg/litre, pyridoxine hydrochloride 0.5 mg/litre, nicotinic acid 0.5 mg/litre, sucrose 30 mg/litre, agar 7 mg/litre.
Owing to have employed technique scheme, the present invention compared with prior art has following useful effect:
Substratum of the present invention and method can shorten the breeding cycle of bosom Cornmarigold largely, and gained seedling early growth is healthy and strong, and growing way is better, and inherited character is more consistent, and the breeding being bosom Cornmarigold is not by the restriction in season, it is possible to big area is promoted.
Embodiment
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1
The present embodiment bosom Cornmarigold tissue culture medium (TCM), comprises bud inducement substratum, root media and strong seedling culture base: bud inducement substratum is taking 2/3MS substratum as minimum medium, adds 50 grams per liter 6-benzyl aminoadenine and 25 grams per liter indolylacetic acids; Root media is taking MS substratum as minimum medium, adds 15 grams per liter Plant hormones regulators,gibberellins, 80 grams per liter glycine and 30 grams per liter peptones; Strong seedling culture base is taking 1/2MS substratum as minimum medium, adds 20 grams per liter naphthylacetic acids, 10 grams per liter indolylacetic acids, 10 grams per liter selenium for egg amido acetic acid and 20 grams per liter indolebutyric acids.
The method of the present embodiment tissue culture is: chooses the tender fresh bosom pearl chrysanthemum of children as explant, with the alcohol disinfecting of 75%, then rinses 3 times with sterile distilled water; It is seeded to bud inducement substratum, natural lighting, it is adjusted to room temperature, cultivate 10 days to growing sprouting; Proceed to root media, natural lighting, regulate temperature to be 22 DEG C, cultivate 13 days to the root growing 1 centimetre; Proceeding to strong seedling culture base, natural lighting, be adjusted to room temperature, cultivate 15 days, grow tall 4 centimetres to seedling, hardening cultivates 15 days.
Embodiment 2
The present embodiment bosom Cornmarigold tissue culture medium (TCM), comprises bud inducement substratum, root media and strong seedling culture base: bud inducement substratum is taking 2/3MS substratum as minimum medium, adds 60 grams per liter 6-benzyl aminoadenine and 20 grams per liter indolylacetic acids; Root media is taking MS substratum as minimum medium, adds 20 grams per liter Plant hormones regulators,gibberellins, 100 grams per liter glycine and 40 grams per liter peptones;Strong seedling culture base is taking 1/2MS substratum as minimum medium, adds 8 grams per liter naphthylacetic acids, 5 grams per liter indolylacetic acids, 3 grams per liter selenium for cystamine hydrochloride and 10 grams per liter indolebutyric acids.
The method of the present embodiment tissue culture is: chooses the tender fresh bosom pearl chrysanthemum of children as explant, with the alcohol disinfecting of 75%, then rinses 4 times with sterile distilled water; It is seeded to bud inducement substratum, natural lighting, it is adjusted to room temperature, cultivate 8 days to growing sprouting; Proceed to root media, natural lighting, regulate temperature to be 26 DEG C, cultivate 14 days to the root growing 2 centimetres; Proceeding to strong seedling culture base, natural lighting, be adjusted to room temperature, cultivate 13 days, grow tall 3 centimetres to seedling, hardening cultivates 17 days.
Embodiment 3
The present embodiment bosom Cornmarigold tissue culture medium (TCM), comprises bud inducement substratum, root media and strong seedling culture base: bud inducement substratum is taking 2/3MS substratum as minimum medium, adds 70 grams per liter 6-benzyl aminoadenine and 10 grams per liter indolylacetic acids; Root media is taking MS substratum as minimum medium, adds 30 grams per liter Plant hormones regulators,gibberellins, 60 grams per liters and 50 grams per liter peptones; Strong seedling culture base is taking 1/2MS substratum as minimum medium, adds 15 grams per liter naphthylacetic acids, 7.5 grams per liter indolylacetic acids, 7 grams per liter selenium for cystamine hydrochloride and 15 grams per liter indolebutyric acids.
The method of the present embodiment tissue culture is: chooses the tender fresh bosom Cornmarigold of children as explant, with the alcohol disinfecting of 75%, then rinses 3 times with sterile distilled water; It is seeded to bud inducement substratum, natural lighting, it is adjusted to room temperature, cultivate 6 days to growing sprouting; Proceed to root media, natural lighting, regulate temperature to be 24 DEG C, cultivate 15 days to the root growing 3 centimetres; Proceeding to strong seedling culture base, natural lighting, be adjusted to room temperature, cultivate 10 days, grow tall 3.5 centimetres to seedling, hardening cultivates 20 days.
Claims (3)
1. cherish the method for Cornmarigold tissue culture for one kind, it is characterised in that comprise the following steps:
A, choose children tender fresh bosom pearl chrysanthemum as explant, with the alcohol disinfecting of 75%, then rinse 3 times～4 times with sterile distilled water;
B, it is seeded to bud inducement substratum, natural lighting, cultivates 6 days～10 days to growing sprouting;
C, proceed to root media, natural lighting, cultivate 13 days～15 days to the root growing 1 centimetre～3 centimetres;
D, proceeding to strong seedling culture base, natural lighting, cultivate 10 days～15 days, grow tall 3 centimetres～4 centimetres to seedling, hardening cultivates 15 days～20 days;
Wherein, bud inducement substratum is taking 2/3MS substratum as minimum medium, adds 50 grams per liter～70 grams per liter 6-benzyl aminoadenine and 10 grams per liter～25 grams per liter indolylacetic acids;
Root media is taking MS substratum as minimum medium, adds 15 grams per liter～30 grams per liter Plant hormones regulators,gibberellins, 60 grams per liter～100 grams per liter glycine and 30 grams per liter～50 grams per liter peptones;
Strong seedling culture base is taking 1/2MS substratum as minimum medium, adds 8 grams per liter～20 grams per liter naphthylacetic acids, 5 grams per liter～10 grams per liter indolylacetic acids, 3 grams per liter～10 grams per liter organoselenium and 10 grams per liter～20 grams per liter indolebutyric acids.
2. the method for bosom according to claim 1 Cornmarigold tissue culture, it is characterised in that described organoselenium is selenium for egg amido acetic acid or selenium for cystamine hydrochloride.
3. the method for bosom Cornmarigold tissue culture according to claims 1 or 2, it is characterised in that: temperature when bud inducement is cultivated is room temperature; During root culture, temperature is 22 DEG C～26 DEG C;Temperature during strong seedling culture is room temperature.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109997695A (en) * | 2019-04-09 | 2019-07-12 | 河南中医药大学 | A kind of bosom chrysanthemum tissue culturing fast seedling-cultivating method |
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| CN101496496A (en) * | 2008-02-02 | 2009-08-05 | 山东省果树研究所 | Tissue culture and rapid propagation method for Gerbera jamesonii Bolus |
| CN102301952A (en) * | 2011-07-19 | 2012-01-04 | 北京林业大学 | Method for breeding chamomile |
| CN102342247A (en) * | 2011-09-28 | 2012-02-08 | 金色种业有限公司 | Selenium-containing MS (Murashige,T. and Skoog,F.) tissue culture medium |
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2016
- 2016-02-26 CN CN201610106436.8A patent/CN105660409A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101496496A (en) * | 2008-02-02 | 2009-08-05 | 山东省果树研究所 | Tissue culture and rapid propagation method for Gerbera jamesonii Bolus |
| CN102301952A (en) * | 2011-07-19 | 2012-01-04 | 北京林业大学 | Method for breeding chamomile |
| CN102342247A (en) * | 2011-09-28 | 2012-02-08 | 金色种业有限公司 | Selenium-containing MS (Murashige,T. and Skoog,F.) tissue culture medium |
Non-Patent Citations (2)
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| 徐艳霞等: "怀珍珠菊离体培养技术研究", 《农技服务》 * |
| 王小菁等编著: "《植物生长调节剂在植物组织培养中的应用》", 31 July 2010, 化学工业出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109997695A (en) * | 2019-04-09 | 2019-07-12 | 河南中医药大学 | A kind of bosom chrysanthemum tissue culturing fast seedling-cultivating method |
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