CN106053188B - A kind of gel dye decoloration integration apparatus and its control method - Google Patents
A kind of gel dye decoloration integration apparatus and its control method Download PDFInfo
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- CN106053188B CN106053188B CN201610478192.6A CN201610478192A CN106053188B CN 106053188 B CN106053188 B CN 106053188B CN 201610478192 A CN201610478192 A CN 201610478192A CN 106053188 B CN106053188 B CN 106053188B
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- 238000012544 monitoring process Methods 0.000 claims abstract description 23
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- Life Sciences & Earth Sciences (AREA)
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- Analytical Chemistry (AREA)
- Biochemistry (AREA)
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- General Physics & Mathematics (AREA)
- Immunology (AREA)
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- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Sampling And Sample Adjustment (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a kind of gels to contaminate decoloration integration apparatus, including gel contaminates decoloration all-in-one machine, control unit；Gel dye decoloration all-in-one machine includes shaking table driving machine, shaking table, contaminates de- cylinder, solenoid valve, video camera and reagent tank；Control unit includes micro-control single-chip microcontroller, and control unit and shaking table driving machine, solenoid valve, liquid level sensor, video camera, alarm device and control panel are electrically connected, and receives input signal, and implement to control；Remote monitoring unit is also set up as supervision equipment, application software is installed thereon, gel dye decoloration integration apparatus can be remotely connected, implement long-range monitoring and control；The present invention can reduce the manual operation of dye decolorization, and dye decoloration control is more accurate, and success rate increases substantially；The contact for reducing human body with pernicious gas, liquid simultaneously, reduces operator's unexpected injury risk；Of the invention novel in design, easy to use, intelligence degree is high, can remote automation operation.
Description
Technical field
The present invention relates to gel-colored and decoloring equipment fields, contaminate decoloration integration apparatus field more particularly to gel.
Background technique
Polyacrylamide gel electrophoresis is referred to as PAGE, is that one kind using polyacrylamide gel as supporting dielectric is common
Electrophoretic techniques, for separating, detecting protein and oligonucleotides.In the preparation process of polyacrylamide gel, coomassie is commonly used
Appropriate decoloration again after bright orchid R250 or G250 is dyed.Currently, the dyeing of polyacrylamide gel and decolorization operations are usually
It is carried out using manual type.Since dyeing and decolorization need continual replacement dyeing liquor or destainer for a long time, manually
Operation will often take quite some effort, and have limitation to the arrangement of time is opposite, once because forgeing or because the time pacifies
Arrange it is unreasonable cause that dyeing liquor or destainer can not be replaced in time, or can not stop in time dyeing or decolorization operations, just hold very much
It easily causes glue to fail, leads to entire the failure of an experiment.On the other hand, manual operation process will inevitably contact various sexual
The reagents such as poison or the dyeing liquor or the destainer that have carcinogenicity, it is very harmful to the physical and mental health of operator.
For this purpose, industry is badly in need of a kind of new type gel dye decoloration integration apparatus that can be remotely operated whenever and wherever possible, solution
Certainly above-mentioned shortcoming.
Summary of the invention
In order to make up for the deficiencies of the prior art, it is an object of the present invention to provide a kind of new type gels to contaminate decoloration integration apparatus,
To solve the problems mentioned in the above background technology, invention thinking of the invention is realization the step of needing manual operation originally
Automation control, specifically setting dyeing liquor, destainer, purified water reagent tank, on liquid effuser road and waste liquid discharge pipe
Road solenoid valve and liquid level sensor is respectively set and realizes automatic control, be arranged above gel slab moveable camera with
When observation Gel Treatment situation and remotely monitor, gel is automatically completed under the control of control panel and remote control module and is contaminated
Decolorization.
To achieve the goals above, technical scheme is as follows:
A kind of gel dye decoloration integration apparatus, for being dyed and being decolourized to gel, it is characterised in that: including gel
Contaminate decoloration all-in-one machine and control unit；Gel dye decoloration all-in-one machine includes shaking table driving machine, shaking table, reagent tank, contaminates de- cylinder, camera shooting
Mechanical, electrical magnet valve and liquid level sensor；Reagent tank includes dyeing liquor reagent tank, destainer reagent tank, purifying water vat, three reagent tanks
It is arranged in gel dye decoloration all-in-one machine top, reagent tank bottom is respectively arranged with pipeline, solenoid valve is respectively arranged on pipeline, tries
Liquid level sensor is respectively arranged on agent casing wall；It is described to contaminate de- cylinder for gel-colored and decoloration vessel, it is arranged on shaking table, shakes
Bed driving machine driving shaking table rotation, contaminates and is provided with liquid level sensor on de- casing wall, contaminate de- cylinder bottom portion and be provided with drain pipe, drain pipe
On be provided with solenoid valve；Video camera is provided with above de- cylinder contaminating, camera arrangements are led in the video camera fixed with crust of the device
On rail, it can be moved along guide rail；Control unit includes micro-control single-chip microcontroller, control unit and shaking table driving machine, solenoid valve, level sensing
Device, video camera, alarm device and control panel are electrically connected, and receive the input signal of liquid level sensor, video camera, control panel,
And solenoid valve, video camera, alarm device are controlled；Micro-control single-chip microcontroller is data processing switching centre, for receiving, analyzing,
Processing sends data and instruction；Control panel is used for input control parameter or outputting operating data.
Further, described contaminate in de- cylinder includes gel slab, liquid level sensor and tilted block；Gel slab is solidifying for placing
Glue, several circular holes are provided on plate, and circular hole is used as fluid passage；Tilted block setting is contaminating bottom in de- cylinder, so that contaminating de- cylinder
Inclined-plane is formed on bottom, and liquid can drain when needing to be discharged.
Further, the reagent tank top open part is provided with a reagent cylinder cap, prevents reagent from volatilizing or spilling.
Further, the micro-control single-chip microcontroller includes communication module, the communication module be WIFI Internet-based without
Line communication module.
It further, further include remote monitoring unit, the remote monitoring unit is mobile phone, tablet computer or calculating
Machine is equipped with application software thereon, and gel dye decoloration integration apparatus can be remotely connected by the communication module, is implemented remote
Journey monitoring and control.
Further, the alarm device is used for alarm when being operating abnormally, including LED light and buzzer.
The control method of above-mentioned gel dye decoloration integration apparatus, which comprises the steps of:
Step 1: preparing dyeing liquor, destainer and purified water, and it is added in corresponding reagent tank；Gel is placed in
On gel slab；
Step 2: opening facility switching, the electric connection of each electric control element is established；
Step 3: passing through control panel or remote monitoring unit to micro-control single-chip microcontroller input control parameter, control parameter packet
It includes but is not limited to: dyeing duration, decoloration duration, decoloration number, shaking speed；
Step 4: activation system is run；
Step 5: micro-control single-chip microcontroller is electrically connected by the program of setting opens reagent tank bottom solenoid valve, dyeing liquor or de-
Color liquid or purified water enter the de- cylinder of dye along pipeline by the program of setting, are dyed or are decolourized or pure to the gel on gel slab
Change water rinsing or purified water saves；
Step 6: level sensing signal is triggered when contaminating the liquid level sensor position that liquid level arrival is arranged inside in de- cylinder,
It is sent to micro-control single-chip microcontroller, micro-control single-chip microcontroller, which is electrically connected, closes reagent tank bottom conduit solenoid valve；
Step 7: micro-control single-chip microcontroller, which is electrically connected to open to contaminate, takes off the drain of cylinder bottom portion when liquid needs to be discharged in the de- cylinder of dye
Solenoid valve on pipe is discharged liquid, and this solenoid valve is automatically closed after draining；
Step 8: remote monitoring unit can connect micro-control single-chip microcontroller, monitoring device operation by communication module whenever and wherever possible
Data remotely obtain gel dye, color throw image by video camera, judge whether adjustment decoloration number or decoloration according to image
Duration etc., and or the long-range operating parameter or control parameter for changing micro-control single-chip microcontroller in real time；
Step 9: indicating that reagent will be used when amount of reagent is less than the liquid level sensor position being arranged inside in reagent tank
To the greatest extent, level sensing signal is triggered, micro-control single-chip microcontroller is sent to, micro-control single-chip microcontroller starts alarm, sends abnormal signal extremely
Remote monitoring unit, and start emergency disposal instruction；
Step 10: starting sound or vibration reminding immediately after remote monitoring unit receives abnormal signal；
Step 11: can remotely check that micro-control single-chip machine emergency disposes situation, and can after operator receives alarm and reminding
By remotely controlling, emergency disposal measure not in place or wrong is corrected immediately；
Step 12: after gel dye, decolorization operations, after operator remotely can check verification result, pass hull closure
Power supply.
The beneficial effects of the present invention are: 1, it contaminates decolorization under the control of system, carries out, subtract automatically by setup parameter
Manual operation is lacked, dye decoloration control is more accurate, and success rate increases substantially；2, it is not necessarily to personnel's watch operation at the scene, is subtracted
The contact for having lacked human body with harmful, carcinogenic gas, liquid, reduces operator's unexpected injury risk；3, present invention design
Novelty, easy to use, intelligence degree is high, can remote automation operation.
Detailed description of the invention
Fig. 1 gel dye decoloration integration apparatus structural schematic diagram；
Fig. 2 gel slab structural schematic diagram；
Fig. 3 control unit connection relationship block diagram.
Wherein: 1- dyes fluid cylinder；2- decoloration fluid cylinder；3- purifies water vat；4- reagent cylinder cap；
51- purified water liquid level sensor；52- destainer liquid level sensor；
53- dyeing liquor liquid level sensor；54- contaminates de- cylinder liquid level sensor；
61- purifies water solenoid valve；62- decoloration liquid electromagnetic valve；63- dyes liquid electromagnetic valve；
64- draining solenoid valve；7- camera guide rails；8- video camera；9- gel slab；10- shaking table；
11- shaking table driving machine；12- control panel；13- remote monitoring unit；14- control unit；
15- tilted block；16- drain pipe；17- gel；18- contaminates de- cylinder；19- pipeline；20- circular hole.
Specific embodiment
The present invention is further detailed with reference to the accompanying drawing.
As shown in Figure 1, gel dye decoloration integration apparatus structural schematic diagram, including gel contaminates decoloration all-in-one machine and control is single
Member.Gel therein dye decoloration all-in-one machine is made of upper and lower two parts, lower half portion include control unit 14, control panel 12,
Shaking table driving machine 11, shaking table 10 contaminate de- cylinder 18, and it includes micro-control single-chip microcontroller on circuit board that control unit 14, which is control circuit board,
It is electrically connected, receives with shaking table driving machine 11, solenoid valve 64, liquid level sensor 54, video camera 8, alarm device and control panel 12
The input of liquid level sensor 51, liquid level sensor 52, liquid level sensor 53, liquid level sensor 54, video camera 8, control panel 12
Signal, and solenoid valve 61, solenoid valve 62, solenoid valve 63, solenoid valve 64, video camera 8, alarm device are controlled；Control panel
12 are arranged in device external, for inputting operation instruction and data；Shaking table driving machine 11 is the power dress that a whole set of implements shaking table
It sets, shaking table 10 is the platform that can be shaken, and is provided with and contaminates de- cylinder 18, shaking table driving for the container of gel dye decoloration
Machine 11 can shake shaking table 10 under the instruction of control unit 14 control, drive the de- cylinder 18 of dye to shake, make dyeing liquor, destainer
It is come into full contact with gel；It contaminates de- cylinder 18 and is internally provided with gel slab 9, for placing gel 17, design has circular hole on gel slab 9
20, it is the flow channel of liquid, contaminating de- 18 bottom of cylinder design has tilted block 15, and the setting of tilted block 15 is contaminating bottom in de- cylinder, making
De- cylinder bottom portion must be contaminated and form inclined-plane, so as to can be emptied when liquid is discharged；It contaminates and is provided with liquid level sensor on de- 18 casing wall of cylinder
54, for monitoring amount of liquid；It contaminates de- 18 side of cylinder and is provided with drain pipe 16, solenoid valve 64, drain pipe are installed on drain pipe 16
16, which pass through device housings, is connected to waste liquid barrel.
Top half includes dyeing fluid cylinder 1, decoloration fluid cylinder 2, purifying water vat 3, reagent cylinder cap 4, liquid level sensor 51, liquid level
Sensor 52, liquid level sensor 53, pipeline 19, solenoid valve 61, solenoid valve 62, solenoid valve 63, camera guide rails 7, video camera 8,
Dyeing fluid cylinder 1, decoloration fluid cylinder 2, purifying water vat 3 are set to upper side, and for storing corresponding reagent, reagent cylinder cap 4 is for preventing
Only reagent volatilization and splashing, liquid level sensor 51, liquid level sensor 52, liquid level sensor 53 are respectively arranged at three reagent tanks
On casing wall, for monitoring amount of liquid, three reagent tank bottoms are provided with pipeline 19, and solenoid valve 61, electricity are respectively arranged on pipeline
Magnet valve 62, solenoid valve 63, for controlling liquid communication cut-off, the public outflux of the liquid of pipeline 19, which is in, contaminates the upper of de- cylinder 18
Side；Camera guide rails 7 and video camera 8 are additionally provided in top half, camera guide rails 7 are fixed with apparatus casing, and video camera 8 can
To be moved on camera guide rails 7 under the control of control unit 14, be aligned the gel 17 being located below contaminate in de- cylinder 18 into
Row is observed and takes pictures.
As shown in Fig. 2, gel slab structural schematic diagram, illustrates the structure of the gel slab 9 for support gel 17, sets thereon
In respect of intensive circular hole 20, the purpose for designing circular hole is not hinder gel 17 to come into full contact with liquid, while liquid can be through circle
It flows in hole 20.
As shown in figure 3, control unit connection relationship block diagram, control unit 14 includes micro-control single-chip microcontroller, communication module, with
Shaking table driving machine 11, solenoid valve 61, solenoid valve 62, solenoid valve 63, solenoid valve 64, liquid level sensor 51, liquid level sensor 52, liquid
Level sensor 53, liquid level sensor 54, video camera 8, alarm device and control panel 12 are electrically connected, reception liquid level sensor 51,
The input signal of liquid level sensor 52, liquid level sensor 53, liquid level sensor 54, video camera 8, control panel 12, and to electromagnetism
Valve 61, solenoid valve 62, solenoid valve 63, solenoid valve 64, video camera 8, alarm device are controlled；Control panel 12 is used for input control
Parameter or outputting operating data；Micro-control single-chip microcontroller be data processing switching centre, for receive, analyze, handle, send data and
Instruction；Micro-control single-chip microcontroller further includes communication module, is connected to the network for establishing with remote monitoring unit, is used in the present embodiment
It is WIFI module；Alarm device is used for alarm when being operating abnormally, including LED light and buzzer；Remote monitoring unit is mobile phone, puts down
Plate computer or computer, are equipped with application software thereon, can remotely connect gel dye decoloration integration apparatus, implement long-range prison
Depending on and control；
In conjunction with Fig. 1: gel dye decoloration integration apparatus structural schematic diagram and Fig. 3: control unit connection relationship block diagram is to have
The step of body running procedure declaration remote automation operation control and method:
Step 1: preparing dyeing liquor, destainer and purified water, and accordingly it is added to dyeing fluid cylinder 1, decoloration fluid cylinder 2, purifying
In water vat 3；Gel 17 is placed on gel slab 9；
Step 2: opening facility switching, the electric connection of each electric control element is established；
Step 3: passing through control panel 12 or remote monitoring unit to micro-control single-chip microcontroller input control parameter, control parameter
Including but not limited to: dyeing duration, decoloration duration, decoloration number, shaking speed；
Step 4: activation system is run；
Step 5: micro-control single-chip microcontroller is electrically connected the solenoid valve opened on reagent tank bottom conduit 19 by the program of setting,
Dyeing liquor or destainer or purified water, which are entered by the program of setting along pipeline 19, contaminates de- cylinder 18, to the gel 17 on gel slab 9
Dyed or decolourized or purified water rinsing or purified water save；
Step 6: when contaminating 5 position of liquid level sensor that liquid level arrival is arranged inside in de- cylinder 18, triggering level sensing letter
Number, it is sent to micro-control single-chip microcontroller, micro-control single-chip microcontroller, which is electrically connected, closes 19 solenoid of reagent tank bottom conduit；
Step 7: micro-control single-chip microcontroller, which is electrically connected to open to contaminate, takes off 18 bottom of cylinder when liquid needs to be discharged in the de- cylinder 18 of dye
Solenoid valve on drain pipe 16 is discharged liquid, and this solenoid valve is automatically closed after draining；
Step 8: remote monitoring unit 13 can connect micro-control single-chip microcontroller, monitoring device fortune by communication module whenever and wherever possible
Row data, by video camera 8 remotely obtain gel dye, color throw image, according to image judge whether adjustment decoloration number or
Duration of decolourizing etc., and or the long-range operating parameter or control parameter for changing micro-control single-chip microcontroller in real time；
Step 9: indicating that reagent will be used when amount of reagent is less than the liquid level sensor position being arranged inside in reagent tank
To the greatest extent, level sensing signal is triggered, micro-control single-chip microcontroller is sent to, micro-control single-chip microcontroller starts alarm, sends abnormal signal extremely
Remote monitoring unit 13, and start emergency disposal instruction；
Step 10: starting sound or vibration reminding immediately after remote monitoring unit receives abnormal signal；
Step 11: can remotely check that micro-control single-chip machine emergency disposes situation, and can after operator receives alarm and reminding
By remotely controlling, emergency disposal measure not in place or wrong is corrected immediately；
Step 12: after gel dye, decolorization operations, after operator remotely can check verification result, pass hull closure
Power supply.
The present embodiment purpose is that professional and technical personnel in the field is allow to understand technical solution of the present invention according to it and adds
To implement, the protection scope of this patent can not be limited with it, it is all to disclose deformation made by technology according to the present invention, each fall within this
Within the protection scope of invention.Protection scope of the present invention is subject to content described in claims.
Claims (6)
1. a kind of control method of gel dye decoloration integration apparatus, the gel dye decoloration integration apparatus include that gel dye is de-
Color all-in-one machine and control unit；Gel dye decoloration all-in-one machine include shaking table driving machine, shaking table, reagent tank, contaminate de- cylinder, video camera,
Solenoid valve and liquid level sensor；Reagent tank includes dyeing liquor reagent tank, destainer reagent tank, purifying water vat, three reagent tank cloth
It is placed in gel dye decoloration all-in-one machine top, reagent tank bottom is respectively arranged with pipeline, solenoid valve, reagent are respectively arranged on pipeline
Liquid level sensor is respectively arranged on casing wall；It is described to contaminate de- cylinder for gel-colored and decoloration vessel, it is arranged on shaking table, shaking table
Driving machine drives shaking table rotation, contaminates and is provided with liquid level sensor on de- casing wall, contaminates de- cylinder bottom portion and is provided with drain pipe, on drain pipe
It is provided with solenoid valve；It is provided with video camera above de- cylinder contaminating, camera arrangements are in the camera guide rails fixed with crust of the device
On, it can be moved along guide rail；Control unit includes micro-control single-chip microcontroller, control unit and shaking table driving machine, solenoid valve, level sensing
Device, video camera, alarm device and control panel are electrically connected, and receive the input signal of liquid level sensor, video camera, control panel,
And solenoid valve, video camera, alarm device are controlled；Micro-control single-chip microcontroller is data processing switching centre, for receiving, analyzing,
Processing sends data and instruction；Control panel is used for input control parameter or outputting operating data；
It is characterized by comprising the following steps:
Step 1: preparing dyeing liquor, destainer and purified water, and it is added in corresponding reagent tank；Gel is placed in gel
On plate；
Step 2: opening facility switching, the electric connection of each electric control element is established；
Step 3: by control panel or remote monitoring unit to micro-control single-chip microcontroller input control parameter, control parameter include but
It is not limited to: dyeing duration, decoloration duration, decoloration number, shaking speed；
Step 4: activation system is run；
Step 5: micro-control single-chip microcontroller is electrically connected by the program of setting opens reagent tank bottom solenoid valve, dyeing liquor or destainer
Or purified water enters the de- cylinder of dye along pipeline by the program of setting, is dyed or is decolourized or purified water to the gel on gel slab
Rinsing or purified water save；
Step 6: triggering level sensing signal when contaminating the liquid level sensor position that liquid level arrival is arranged inside in de- cylinder, send
To micro-control single-chip microcontroller, micro-control single-chip microcontroller, which is electrically connected, closes reagent tank bottom conduit solenoid valve；
Step 7: micro-control single-chip microcontroller, which is electrically connected to open to contaminate, to be taken off on cylinder bottom drain when liquid needs to be discharged in the de- cylinder of dye
Solenoid valve, liquid is discharged, and this solenoid valve is automatically closed after draining；
Step 8: remote monitoring unit can whenever and wherever possible by communication module connect micro-control single-chip microcontroller, monitoring device operation data,
Gel dye, color throw image are remotely obtained by video camera, and adjustment decoloration number or decoloration duration are judged whether according to image,
And the operating parameter or control parameter of the long-range micro-control single-chip microcontroller of change in real time；
Step 9: indicating that reagent will be used up when amount of reagent is less than the liquid level sensor position being arranged inside in reagent tank, touching
Lotion position transducing signal is sent to micro-control single-chip microcontroller, and micro-control single-chip microcontroller starts alarm, sends abnormal signal to long-range prison
Unit is controlled, and starts emergency disposal instruction；
Step 10: starting sound or vibration reminding immediately after remote monitoring unit receives abnormal signal；
Step 11: can remotely check that micro-control single-chip machine emergency disposes situation, and can pass through after operator receives alarm and reminding
Long-range control, corrects emergency disposal measure not in place or wrong immediately；
Step 12: after gel dye, decolorization operations, after operator remotely can check verification result, pass hull closure power supply.
2. a kind of control method of gel dye decoloration integration apparatus according to claim 1, which is characterized in that the dye
It include gel slab, liquid level sensor and tilted block in de- cylinder；Gel slab is used to place gel, several circular holes are provided on plate,
Circular hole is used as fluid passage；Tilted block setting is contaminating bottom in de- cylinder, so that contaminating de- cylinder bottom portion forms inclined-plane, liquid is needing to arrange
It can be drained when out.
3. a kind of control method of gel dye decoloration integration apparatus according to claim 1, which is characterized in that the examination
Agent cylinder top open part is provided with a reagent cylinder cap, prevents reagent from volatilizing or spilling.
4. a kind of control method of gel dye decoloration integration apparatus according to claim 1, which is characterized in that described micro-
Controlling single-chip microcontroller includes communication module, and the communication module is WIFI wireless communication module Internet-based.
5. a kind of control method of gel dye decoloration integration apparatus according to claim 1, which is characterized in that described
Remote monitoring unit is mobile phone, tablet computer or computer, is equipped with application software thereon, can be remote by the communication module
Journey connects gel dye decoloration integration apparatus, implements long-range monitoring and control.
6. a kind of control method of gel dye decoloration integration apparatus according to claim 1, which is characterized in that the police
Report device is used for alarm when being operating abnormally, including LED light and buzzer.
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| CN107064497B (en) * | 2017-06-13 | 2019-03-01 | 福建省农业科学院畜牧兽医研究所 | A kind of portable electrophoresis and photographing device and its application method |
| CN107271419B (en) * | 2017-07-24 | 2020-06-19 | 左豫虎 | Gel dyeing scanning imaging device and dyeing imaging method thereof |
| CN110132695B (en) * | 2019-05-27 | 2022-01-11 | 福州迈新生物技术开发有限公司 | Method for reducing abnormal dyeing caused by human error by pathological dyeing system |
| CN111323285B (en) * | 2020-03-11 | 2022-12-06 | 桂林医学院 | Automatic change dyeing decoloration device |
| CN111537591B (en) * | 2020-05-13 | 2022-09-13 | 桂林医学院 | Dyeing and decoloring system |
| CN111562159B (en) * | 2020-05-13 | 2023-01-20 | 桂林医学院 | Working method of dyeing and decoloring equipment |
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