CN101464236A - Cell tissue dyeing method and dyeing machine - Google Patents
Cell tissue dyeing method and dyeing machine Download PDFInfo
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- CN101464236A CN101464236A CNA2009100604759A CN200910060475A CN101464236A CN 101464236 A CN101464236 A CN 101464236A CN A2009100604759 A CNA2009100604759 A CN A2009100604759A CN 200910060475 A CN200910060475 A CN 200910060475A CN 101464236 A CN101464236 A CN 101464236A
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- slide
- staining
- dye
- transition block
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- 238000000034 method Methods 0.000 title claims abstract description 19
- 238000004043 dyeing Methods 0.000 title claims description 67
- 239000007788 liquid Substances 0.000 claims abstract description 62
- 230000007704 transition Effects 0.000 claims abstract description 39
- 239000012530 fluid Substances 0.000 claims description 31
- 238000010079 rubber tapping Methods 0.000 claims description 12
- 238000010438 heat treatment Methods 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 230000003068 static effect Effects 0.000 claims description 5
- 210000001503 joint Anatomy 0.000 claims description 3
- 238000010186 staining Methods 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 9
- 230000033001 locomotion Effects 0.000 abstract description 5
- 230000008569 process Effects 0.000 abstract description 5
- 239000012192 staining solution Substances 0.000 abstract 5
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 238000007447 staining method Methods 0.000 abstract 1
- 239000000975 dye Substances 0.000 description 128
- 101100204059 Caenorhabditis elegans trap-2 gene Proteins 0.000 description 11
- 239000004065 semiconductor Substances 0.000 description 6
- 238000004040 coloring Methods 0.000 description 3
- 238000007599 discharging Methods 0.000 description 3
- 239000000984 vat dye Substances 0.000 description 3
- 208000002599 Smear Layer Diseases 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000002706 hydrostatic effect Effects 0.000 description 2
- 230000002452 interceptive effect Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000005476 soldering Methods 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 230000007306 turnover Effects 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000006855 networking Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
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Abstract
The invention provides a cell tissue staining method which can keep a slide still and control the flow mode of a staining solution, and a staining machine thereof. The staining machine mainly comprises a liquid storage tank, a solenoid valve, a dye vat, a machine frame, a control system, a transition block and a liquid collection device; the liquid storage tank is positioned at the upper part of the machine frame; the dye vat is positioned at the lower part of the machine frame; the liquid collection device and the solenoid valve are positioned between the liquid storage tank and the dye vat; the liquid storage tank is connected with the liquid collection device through the solenoid valve; and the liquid collection device is connected with the dye vat through the transition block. The method is characterized in that the natural liquid level pressure difference of the staining solution serves as a liquid working power source during the entire process of staining; the staining solution in the liquid storage tank flows into the liquid collection device through the solenoid valve; the staining solution in the liquid collection device is heated to the temperature set for staining, and then flows into the dye vat provided by a slide through the transition valve; and the staining process is completed by the slide in the dye vat. Compared with the traditional staining machine which keeps staining solution still and controls the model of slide movement, the invention is advanced and can achieve the effects of enhancing staining effect, improving staining efficiency, saving energy, reducing pollution and saving staining cost.
Description
Technical field
The present invention relates to the cell of a kind of health care and biological study technical field, the colouring method and the equipment of biological tissue, particularly relate to cell tissue dyeing method and overflow dyeing machine thereof.
Background technology
Till settled the present, cell, biological tissue's colouring method and device category are fewer, the cell tissue dyeing equipment of Shi Yonging mainly is that the slide that will scribble cell tissue places respectively and fills the various dye liquors dye vat of (dyestuff and auxiliary liquid claim dye liquor) on the market, utilize mechanical hook-up to control one group of slide, move to each dye vat top by the dyeing procedure requirement, VTOL (vertical take off and landing) is soaked in slide in the dye liquor then, repeats above action again after reaching soak time.
This colouring method and device have following shortcoming, and be especially more apparent outstanding when staining procedure is many.
1, because dye liquor can not in time be changed, and dye liquor effective constituent progressively reduces before dye liquor effectiveness exhausts, the Color extreme difference, and also the front and back Color is inconsistent;
2, owing to will control each dye vat temperature, control slide (frame) translation and lifting again, so energy consumption height, noise are big, make that also equipment volume is excessive, cost is high simultaneously;
3, dyeing course can only be done a kind of dyeing, as the another kind of dyeing of needs, then needs to change multiple dye liquor, complicated operation.
Summary of the invention
The purpose of this invention is to provide a kind of slide is static, control the dye liquor type of flow cell tissue dyeing method and overflow dyeing machine thereof, its changes, and traditional overflow dyeing machine dye liquor is static, the mode of the mobile slide of control, can reach improve Color, improve dyeing efficient, effect that energy savings, minimizing are polluted, saved the dyeing cost.
In order to achieve the above object, cell tissue dyeing method of the present invention, it is characterized in that: slide is static, control dye liquor flows, the different dye liquors that are contained in the fluid reservoir flow into liquid trap successively through operation valve, dye liquor is warmed to the dyeing setting in liquid trap temperature flows into the dye vat that slide is housed through transition block again, and slide is finished dyeing course in dye vat.
Cell tissue dyeing machine of the present invention mainly comprises fluid reservoir, solenoid valve, dye vat, frame and control system, it is characterized in that: also comprise transition block and liquid trap;
Fluid reservoir is positioned at upper rack, and dye vat is positioned at frame lower, and liquid trap, solenoid valve are between fluid reservoir and dye vat, and fluid reservoir is connected with solenoid valve with liquid trap, and liquid trap is connected with transition block with dye vat.
Dye vat of the present invention is the square box that forms at the bottom of four walls and, wherein two cornices of length direction have slot, slot is staggered relatively, the slot lateral length is the length of slide, it highly is the slide width, the width of every groove is the thickness of one or two slide, the big I of dye vat is made into different size according to the size of slide and the size of dyeing amount, the heating plate that dye vat is installed below, the dye vat bottom inside has a cannelure, and front of the slot has two apertures, and aperture is used for entering and discharging of dye liquor, between the every groove of dye vat is vertical gapped (gap of liquid inflow and outflow), few when the gap is very little to the liquid requirement that participates in dyeing.
Transition block of the present invention comprises transition block, feed liquor valve, tapping valve and O-ring seal, feed liquor valve, tapping valve and O-ring seal are installed on the transition block, feed liquor valve control dye liquor enters dye vat, tapping valve control dye liquor is discharged from dye vat, the effect of O-ring seal is to make dye vat be connected good sealing effect with transition block, do not leak dye liquor, at the tapping valve end draining water pump is arranged, to accelerate drainage speed and to drain raffinate, realize connecting fast with the O-ring seal depended on pressure between dye vat and the transition block, dye vat can freely take off, and changes slide.
Dye vat of the present invention is one or more, connects each dye vat and is furnished with solenoid valve, liquid trap and transition block respectively, and dye vat manually docks with transition block or adopts the gearing butt joint, and each dye vat is carried out dyeing procedure separately, reaches different Colors.
Gearing of the present invention is mainly by driving motor, gear, driving rack, base plate, sliding panel and travel switch are formed, driving motor is installed on the base plate, gear is installed on the motor shaft, driving rack is installed on the sliding panel, between base plate and the sliding panel for being slidingly connected, gear and driving rack engagement, travel switch is installed on the base plate, the rotation driven gear of driving motor rotates, gear rotates and drives the driving rack rectilinear motion, the motion of driving rack drives sliding panel and slides on base plate, the turnover of the control dye vat that moves back and forth of sliding panel, and the effect of travel switch is make the sliding panel sliding stroke spacing.
Control system of the present invention comprises program control system, fluid level controller system, temperature controller system and warning device.
Program control system is to adopt PLC Programmable Logic Controller and touch display screen to carry out program process to set control and interactive system, the dyeing order of control cell tissue, dyeing temperature and time, the fluid level controller system is meant a contactless liquid level sensor in the liquid trap outside, can according to dyeing slide what, the height of regulator solution level sensor, open and close by the programmed control solenoid valve, control enters the consumption of dye vat dye liquor, temperature controller system is meant the temperature sensor of installing in dye vat, the heating plate that dye vat is installed below, the temperature of temperature sensor senses dye vat dye liquor, whether open by program control system control heating plate, to keep dye vat to dye the dyeing working temperature that temperature meets setting, warning device is meant that 1 liquid level sensor is arranged at the bottom in each fluid reservoir, when lacking liquid in the fluid reservoir, liquid level sensor sends alerting signal, and on touch display screen, show scarce liquid, replenish dye liquor so that remind.
Fluid reservoir of the present invention is the container of an opened upper end, and a connecting pipe is arranged at the bottom, and connecting pipe is used for being connected with solenoid valve, and fluid reservoir bottoms level sensor is used for detecting fluid reservoir and whether also has dye liquor.
Liquid trap of the present invention is a hydrostatic column, and top links to each other with solenoid valve, and the bottom links to each other with transition block, and dye liquor is stored in the liquid trap before flowing into dye vat, and semiconductor heats up and heat sink makes dye liquor reach the working temperature of setting by being provided with in the cabinet.
Solenoid valve of the present invention is the bi-bit bi-pass solenoid electric valve, opens and closes by control electromagnetic valve, and what of dye vat dye liquor are may command flow into.
Frame of the present invention is the supporting device of each parts unit device, is stainless steel soldering group component, semiconductor is set in frame heats up and heat sink.
Dye liquor of the present invention comprises all liq of using in the dyeing such as dyeing liquid, cleaning liquid.
During work, the slide of cell tissue will be scribbled, every or per two leans against privately and overlaps, become one group, be that slide does not have smear layer and overlaps, dyeing face outside, then with in every group of slot that inserts the slide dye vat respectively, the dye vat that slide is housed is installed on the gearing, and gearing starts makes dye vat closely be connected with transition block, by set the program of dyeing at display screen, time and temperature, the control system control electromagnetic valve, the open and close of semiconductor intensification and heat sink, make the dye liquor in the dye vat arrive temperature and the consumption of setting, dye liquor is full of the slide gap after injecting dye vat, and the cell tissue on the slide just is colored.
In dyeing course, pass in the minimum gap of dye liquor slide in dye vat, thereby the dye liquor consumption is seldom, and floats hourly water consumption clearly seldom at clear water, not only save the dye liquor cost but also saved water resource, reduce the environmental pollution that discharging of waste liquid causes significantly; In dyeing course, do not have gearings such as translation, lifting, do not have powerful heating and cooling equipment, thereby save a large amount of energy, the noise in the minimizing work; Because the dye liquor consumption is few in the course of work, machinery is not mobile up and down again for slide, and corresponding fluid reservoir volume is very little, so equipment volume reduces at double; Use micro-dye liquor dyeing, can directly be discharged in the waste collection case with the dye liquor of crossing, do not reuse, working temperature is controlled simultaneously, so splendid to the consistance and the repeatability of Color; Because dye vat accessory kind is more, installing/dismounting is simple, thus have very high dirigibility and high work efficiency, each work can handle slide quantity from several to 100 multi-discs even more, and dyeing time is identical; Adopt the PLC industrial computer to control and man-machine interface, dyeing course is carried out sequencing control, dyeing order, temperature and time are regulated very easy, and safeguard in all right simultaneously networking control and strange land.
Description of drawings
Fig. 1 is the forward sight shaft side figure of the embodiment of cell tissue dyeing machine of the present invention.
Fig. 2 is the backsight shaft side figure of the embodiment of the invention.
Fig. 3 is the dye vat structural drawing of the embodiment of the invention.
Fig. 4 is the dye liquor schematic flow sheet of the embodiment of the invention.
Fig. 5 is the gearing synoptic diagram of the embodiment of the invention.
Fig. 6 is the structural scheme of mechanism of transition block 4 among Fig. 1.
Embodiment
The explanation of mark among Fig. 1: fluid reservoir 1, liquid trap 2, solenoid valve 3, transition block 4, dye vat 5, gearing 6, frame 7, display screen 8, heating plate 9, following hatch door 10, last hatch door 11, gear 24.
The explanation of mark among Fig. 2: draining water pump 12, control system 13, power supply 14.
The explanation of mark among Fig. 3: dye vat advances freeing port 5.1.
The explanation of mark among Fig. 4: liquid level sensor 15, connecting pipe 16.
The explanation of mark among Fig. 5: driving rack 17, travel switch 18, O-ring seal 19, driving motor 20, sliding panel 25, base plate 26.
The explanation of mark among Fig. 6: tapping valve 21, feed liquor valve 22, transition block 23.
Referring to Fig. 4, cell tissue dyeing method of the present invention, it is characterized in that: slide is static, control dye liquor flows, the different dye liquors that are contained in the fluid reservoir 1 flow into liquid trap 2 successively through solenoid valve 3, dye liquor is warmed to the dyeing setting in liquid trap 2 temperature flows into the dye vat 5 that slide is housed through transition block 4 again, and slide is finished dyeing course for 5 li at dye vat.
Referring to Fig. 1, cell tissue dyeing machine of the present invention mainly comprises fluid reservoir 1, solenoid valve 3, dye vat 5, frame 7 and control system 13, also comprises transition block 4 and liquid trap 2, and fluid reservoir 1 is positioned at frame 7 tops, and dye vat 5 is positioned at frame 7 bottoms.This cell tissue dyeing machine is to utilize the liquid working power of the natural liquid level pressure differential of dye liquor as the dyeing overall process.
Referring to Fig. 4, liquid trap 2, solenoid valve 3 are between fluid reservoir 1 and dye vat 5, and fluid reservoir 1 is connected with liquid trap 2 usefulness solenoid valves 3, and liquid trap 2 is connected with dye vat 5 usefulness transition block 4.
Referring to Fig. 3, dye vat 5 is the square box that forms at the bottom of four walls and, wherein two cornices of length direction have slot, slot is staggered relatively, the slot lateral length is the length of slide, it highly is the slide width, the width of every groove is the thickness of one or two slide, the big I of dye vat 5 is made into different size according to the size of slide and the size of dyeing amount, the heating plate 9 that dye vat 5 is installed below, dye vat 5 bottom inside have a cannelure, it is that dye vat advances freeing port 5.1 that front of the slot has two apertures, dye vat advances freeing port 5.1 and is used for entering and discharging of dye liquor, and is between dye vat 5 every grooves are vertical gapped (gap of liquid inflow and outflow), few to the liquid requirement that participates in dyeing when the gap is very little.
Referring to Fig. 6, transition block 4 comprises transition block 23, feed liquor valve 22, tapping valve 21 and O-ring seal 19, feed liquor valve 22, tapping valve 21 and O-ring seal 19 are installed on the transition block 23, feed liquor valve 22 control dye liquors enter dye vat 5, tapping valve 21 control dye liquors are discharged from dye vat 5, the effect of O-ring seal 19 is to make dye vat 5 be connected good sealing effect with transition block 4, do not leak dye liquor, at tapping valve 21 ends draining water pump 12 is arranged, to accelerate drainage speed and to drain raffinate, realize connecting fast with O-ring seal 19 depended on pressure between dye vat 5 and the transition block 4, dye vat 5 can freely take off, and changes slide.
Referring to Fig. 5, gearing 6 is mainly by driving motor 20, gear 24, driving rack 17, base plate 26, sliding panel 25 and travel switch 18 are formed, driving motor 20 is installed on the base plate 26, gear 24 is installed on the motor shaft, driving rack 17 is installed on the sliding panel 25, between base plate 26 and the sliding panel 25 for being slidingly connected, gear 24 and driving rack 17 engagements, travel switch 18 is installed on the base plate 26, the rotation driven gear 24 of driving motor 20 rotates, gear 24 rotates and drives driving rack 17 rectilinear motions, the motion of driving rack 17 drives sliding panel 25 and slides on base plate 26, the turnover of the control dye vat 5 that moves back and forth of sliding panel 25, the effect of travel switch 18 is to make sliding panel 25 sliding strokes spacing.
During work, the slide of cell tissue will be scribbled, in the every slot of packing into separately, or per two lean against privately and overlap, become one group, be that slide does not have smear layer and overlaps, dyeing face outside, then with in every group of slot that inserts slide dye vat 5 respectively, the dye vat 5 that slide is housed is installed on the gearing 6, gearing 6 starts makes dye vat 5 closely be connected with transition block 4, by set the program of dyeing at display screen 8, time and temperature, control system 13 control electromagnetic valve 3, the open and close of semiconductor intensification and heat sink make the dye liquor of 5 li in dye vat arrive temperature and the consumption of setting, dye liquor is full of the slide gap after injecting dye vat 5, and the cell tissue on the slide just is colored.
Cell tissue dyeing method and overflow dyeing machine thereof are applied health care and biological study technical field, can improve Color, raising dyeing efficient, energy savings, the minimizing of cell tissue greatly and pollute, save the dyeing cost.
Claims (6)
1, cell tissue dyeing method, it is characterized in that: slide is static, control dye liquor flows, the different dye liquors that are contained in the fluid reservoir flow into liquid trap successively through operation valve, dye liquor is warmed to the dyeing setting in liquid trap temperature flows into the dye vat that slide is housed through transition block again, and slide is finished dyeing course in dye vat.
2, cell tissue dyeing machine mainly comprises fluid reservoir, solenoid valve, dye vat, frame and control system, it is characterized in that: also comprise transition block and liquid trap;
Fluid reservoir is positioned at upper rack, and dye vat is positioned at frame lower, and liquid trap, solenoid valve are between fluid reservoir and dye vat, and fluid reservoir is connected with solenoid valve with liquid trap, and liquid trap is connected with transition block with dye vat.
3, cell tissue dyeing machine according to claim 2, it is characterized in that: dye vat is the square box that forms at the bottom of four walls and, wherein two cornices of length direction have slot, slot is staggered relatively, the slot lateral length is the length of slide, it highly is the slide width, the width of every groove is the thickness of one or two slide, the size of dye vat is made into different size according to the size of slide and the size of dyeing amount, the heating plate that dye vat is installed below, the dye vat bottom inside has a cannelure, and front of the slot has two apertures, and is gapped between the every groove of dye vat is vertical.
4, cell tissue dyeing machine according to claim 2, it is characterized in that: transition block comprises transition block, feed liquor valve, tapping valve and O-ring seal, feed liquor valve, tapping valve and O-ring seal are installed on the transition block, at the tapping valve end draining water pump is arranged, between dye vat and the transition block with O-ring seal by connecting.
5, cell tissue dyeing machine according to claim 2 is characterized in that: dye vat is one or more, connects each dye vat and is furnished with solenoid valve, liquid trap and transition block respectively, and dye vat manually docks or adopt the gearing butt joint with transition block.
6, cell tissue dyeing machine according to claim 5, it is characterized in that: gearing mainly is made up of driving motor, gear, driving rack, base plate, sliding panel and travel switch, driving motor is installed on the base plate, gear is installed on the motor shaft, driving rack is installed on the sliding panel, for being slidingly connected, gear and driving rack mesh between base plate and the sliding panel, and travel switch is installed on the base plate.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100604759A CN101464236B (en) | 2009-01-09 | 2009-01-09 | Cell tissue dyeing method and dyeing machine |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100604759A CN101464236B (en) | 2009-01-09 | 2009-01-09 | Cell tissue dyeing method and dyeing machine |
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| CN101464236A true CN101464236A (en) | 2009-06-24 |
| CN101464236B CN101464236B (en) | 2012-06-27 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102645682A (en) * | 2012-04-26 | 2012-08-22 | 厦门雅瑞光学有限公司 | Dyeing method and dyeing device of gradient eyeglass |
| CN103323313A (en) * | 2012-03-23 | 2013-09-25 | 黄伏生 | Liquid-based cell analyzer staining method of cells exfoliated from serous cavity by using Wright staining method |
| CN105259009A (en) * | 2015-11-11 | 2016-01-20 | 刘洋 | Automatic control system used for section staining |
| CN105571925A (en) * | 2016-03-09 | 2016-05-11 | 福州迈新生物技术开发有限公司 | Dyeing module applied to biological samples on glass slides and dyeing method thereof |
| CN106053188A (en) * | 2016-06-24 | 2016-10-26 | 广东医学院 | Gel dyeing-decoloration integrated equipment and control method thereof |
| CN107478485A (en) * | 2017-06-30 | 2017-12-15 | 严铭 | Gram's staining instrument |
| CN107843458A (en) * | 2017-11-02 | 2018-03-27 | 牡丹江医学院 | A kind of preclinical medicine check sampling device |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201344875Y (en) * | 2009-01-09 | 2009-11-11 | 孙小蓉 | Cell tissue staining machine |
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2009
- 2009-01-09 CN CN2009100604759A patent/CN101464236B/en active Active
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103323313A (en) * | 2012-03-23 | 2013-09-25 | 黄伏生 | Liquid-based cell analyzer staining method of cells exfoliated from serous cavity by using Wright staining method |
| CN103323313B (en) * | 2012-03-23 | 2016-06-29 | 黄伏生 | Rui Shi method is for the serous cavity exfoliative cyte colouring method of liquid basal cell instrument |
| CN102645682A (en) * | 2012-04-26 | 2012-08-22 | 厦门雅瑞光学有限公司 | Dyeing method and dyeing device of gradient eyeglass |
| CN102645682B (en) * | 2012-04-26 | 2014-08-27 | 厦门雅瑞光学有限公司 | Dyeing method and dyeing device of gradient eyeglass |
| CN105259009A (en) * | 2015-11-11 | 2016-01-20 | 刘洋 | Automatic control system used for section staining |
| CN105571925A (en) * | 2016-03-09 | 2016-05-11 | 福州迈新生物技术开发有限公司 | Dyeing module applied to biological samples on glass slides and dyeing method thereof |
| CN105571925B (en) * | 2016-03-09 | 2018-02-09 | 福州迈新生物技术开发有限公司 | The staining modules and its colouring method of biological sample on slide |
| CN106053188A (en) * | 2016-06-24 | 2016-10-26 | 广东医学院 | Gel dyeing-decoloration integrated equipment and control method thereof |
| CN106053188B (en) * | 2016-06-24 | 2019-01-29 | 广东医科大学 | A kind of gel dye decoloration integration apparatus and its control method |
| CN107478485A (en) * | 2017-06-30 | 2017-12-15 | 严铭 | Gram's staining instrument |
| CN107843458A (en) * | 2017-11-02 | 2018-03-27 | 牡丹江医学院 | A kind of preclinical medicine check sampling device |
| CN107843458B (en) * | 2017-11-02 | 2019-11-08 | 牡丹江医学院 | A kind of preclinical medicine check sampling device |
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| CN101464236B (en) | 2012-06-27 |
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Owner name: WUHAN LANDING MEDICAL HI-TECH CO., LTD. Free format text: FORMER OWNER: SUN XIAORONG Effective date: 20130726 Free format text: FORMER OWNER: YUN JIABAO Effective date: 20130726 |
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Denomination of invention: Cell tissue dyeing method and dyeing machine Effective date of registration: 20150327 Granted publication date: 20120627 Pledgee: Wuhan rural commercial bank Limited by Share Ltd Optics Valley branch Pledgor: Wuhan Landing Medical Hi-tech Ltd. Registration number: 2015990000237 |
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Date of cancellation: 20160129 Granted publication date: 20120627 Pledgee: Wuhan rural commercial bank Limited by Share Ltd Optics Valley branch Pledgor: Wuhan Landing Medical Hi-tech Ltd. Registration number: 2015990000237 |
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Denomination of invention: Cell tissue dyeing method and dyeing machine Effective date of registration: 20160201 Granted publication date: 20120627 Pledgee: Wuhan rural commercial bank Limited by Share Ltd Optics Valley branch Pledgor: Wuhan Landing Medical Hi-tech Ltd. Registration number: 2016420000003 |
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Date of cancellation: 20180124 Granted publication date: 20120627 Pledgee: Wuhan rural commercial bank Limited by Share Ltd Optics Valley branch Pledgor: Wuhan Landing Medical Hi-tech Ltd. Registration number: 2016420000003 |
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Denomination of invention: Cell tissue dyeing method and dyeing machine Effective date of registration: 20180419 Granted publication date: 20120627 Pledgee: Wuhan rural commercial bank Limited by Share Ltd Optics Valley branch Pledgor: Wuhan Landing Medical Hi-tech Ltd. Registration number: 2018420000018 |
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Date of cancellation: 20191213 Granted publication date: 20120627 Pledgee: Guanggu Branch of Wuhan Rural Commercial Bank Co., Ltd. Pledgor: Wuhan Landing Medical Hi-tech Ltd. Registration number: 2018420000018 |
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Address after: 430000 B7, C and D units, one or two floors, medical equipment Park, 818 hi tech Avenue, East Lake New Technology Development Zone, Hubei, Wuhan, Patentee after: Wuhan Lanting intelligent Medicine Co., Ltd Address before: 430206, Hubei province Wuhan East Lake New Technology Development Zone 818 high-tech Avenue, medical equipment Park B7, B, C, D zone one or two Patentee before: WUHAN LANDING MEDICAL HI-TECH Ltd. |