CN210630381U

CN210630381U - Be suitable for experimental device of evaluation mouse fatigue-alleviating function

Info

Publication number: CN210630381U
Application number: CN201921181946.7U
Authority: CN
Inventors: 曲敏; 李阳; 樊柏林; 夏莹; 田辉
Original assignee: HUBEI PROVINCIAL CENTER FOR DISEASE CONTROL AND PREVENTION
Current assignee: HUBEI PROVINCIAL CENTER FOR DISEASE CONTROL AND PREVENTION
Priority date: 2019-07-25
Filing date: 2019-07-25
Publication date: 2020-05-29
Anticipated expiration: 2029-07-25

Abstract

The utility model discloses a device suitable for evaluating a mouse fatigue relieving function test and a using method thereof, comprising a water tank, a water tank control cabinet, a heating temperature control device, N timing devices, a video monitoring device, a water circulating pump and a PC machine, wherein the water tank comprises a square outer frame, a square inner clapboard with holes, a square inner gridding frame and a plurality of swimming cylinders, wherein the square inner clapboard with holes is arranged in the square outer frame; the heating temperature control device is used for keeping the water temperature in the water tank in a constant temperature state, each timing device comprises an independent timer and an independent timer, corresponds to one swimming barrel and is used for recording the weight swimming or the non-weight swimming of the mouse; the video monitoring device is arranged above the outer frame wall of the water tank and is used for observing the swimming test state of the mouse; the water circulating pump is used for ensuring that water in the water tank is in a circulating flow state and preventing the mouse from floating. The utility model has the advantages of simple structure, convenient assembly and disassembly, cleaning, convenient part replacement and wide test range.

Description

Be suitable for experimental device of evaluation mouse fatigue-alleviating function

Technical Field

The utility model relates to an animal is alleviated tired function test's device, specific saying so relates to a device that is suitable for evaluation mouse and alleviates tired function test and application method thereof.

Background

In the field of life science research, a mouse swimming experiment is an important means for researching an anti-fatigue function, the size of exercise endurance is reflected by the swimming time of an experimental animal, and the method is a very visual method for evaluating exercise-induced fatigue. In the past physical fatigue test for alleviating mice, a common iron water bucket is generally adopted for a swimming water tank for a mouse endurance test, and a plurality of mice are placed in one water bucket, so that the following problems generally exist: in the swimming process, mice mutually interfere, so that the animal numbers are blurred and cannot be independently timed; the water temperature in the water barrel can fluctuate under the influence of the external environment, and the accuracy of test results and data can be further influenced.

Chinese patent CN207022900U discloses a mouse constant temperature swimming tester, including the shell, set up be used for the reservoir of water storage in the shell, N vertically set up on the shell and with reservoir sealing connection's swimming cover, set up the heating rod in the reservoir, set up switch on the shell, set up respectively in every N peristaltic pump of swimming cover below and set up the peristaltic pump switch on the shell, N is more than or equal to 2's natural number, the swimming cover is the cylindrical structure, the heating rod passes through switch and connects the power, the peristaltic pump passes through peristaltic pump switch and connects the power. In order to avoid the force of the mouse relying on the inner wall of the swimming cover, a conical cone cap is arranged above the swimming cover. In order to accurately control the water temperature, the water storage device also comprises a temperature sensor arranged in the water storage tank and a temperature display regulator arranged on the shell, wherein the temperature sensor is connected with the temperature display regulator. For convenience of timing, the swimming goggles further comprise N timers which are installed on the shell, and the N timers are installed corresponding to the N swimming hoods respectively. In order to facilitate water exchange, the water storage tank also comprises a drain hole which is arranged at the bottom of the shell and communicated with the water storage tank. In order to improve the safety, the swimming pool also comprises a partition board which is horizontally arranged in the water storage pool and is positioned between the swimming cover and the heating rod, and a plurality of through holes are arranged on the partition board.

Although the above patent provides that the mutual interference between mice is solved through setting up N swimming covers, lead to the unclear problem of animal number, through being equipped with a time-recorder for every swimming cover and solve the problem that can't time alone and adjust the temperature through heating rod, temperature sensor and temperature display regulator, solve the temperature in the cask and can receive the influence of external environment and produce undulant problem, its structure is more complicated, it is inconvenient to install and remove the washing, secondly, need the operator to be on the side at any time at whole testing process, can not real-time recording, save the experimental state of mouse in testing process, the function is single, only can be used for recording mouse heavy burden swimming test, do not possess the function of counting down, be not convenient for the mouse not heavy burden swimming test and survey the experiment of serum urea or blood lactic acid.

Disclosure of Invention

To the problem that exists among the background art, the utility model aims to provide a simple structure, ann tear open, wash the convenience and take automatic temperature control function, rivers circulation function, timing function and countdown function's suitable for evaluation mouse to alleviate fatigue function test's device and method of use.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a device suitable for evaluating a mouse fatigue-relieving function test comprises a water tank, a water tank control cabinet, a heating temperature control device, N timing devices, a video monitoring device, a water circulating pump and a PC (personal computer); wherein:

the water tank comprises a square outer frame, and a square inner partition plate with holes, a square inner grid frame and N swimming barrels which are arranged in the square outer frame, wherein the inner cavity of the square outer frame is divided into an upper interval and a lower interval by the square inner partition plate with holes; the square inner grid frame is positioned in an upper section of the inner cavity of the square outer frame, the upper section of the inner cavity of the square outer frame is divided into a plurality of square swimming areas, and a swimming barrel is embedded in each square swimming area;

the heating temperature control device comprises a water temperature sensor, an electric heater and a temperature digital display regulator, wherein the water temperature sensor and the electric heater are arranged in the lower section of the inner cavity of the square outer frame, and the temperature digital display regulator is arranged on the water tank control cabinet; the water temperature sensor and the electric heater are both electrically connected with the temperature digital display regulator, the temperature digital display regulator is electrically connected with a temperature control switch arranged on the water tank control cabinet, the temperature control switch is electrically connected with a power interface arranged on the water tank control cabinet, and the power interface is electrically connected with an external power supply through a power line;

the N timing devices are all arranged on the water tank control cabinet, each timing device is matched and corresponding to one swimming barrel in the water tank and is electrically connected with a PLC (programmable logic controller) arranged in the water tank control cabinet, each timing device comprises a digital display timer, a warning lamp A, a warning lamp B, a buzzer A and a buzzer B, and the warning lamp A, the warning lamp B, the buzzer A and the buzzer B in each timing device are electrically connected with the corresponding digital display timer); the PLC is electrically connected with the PC through a PC data interface arranged on the water tank control cabinet;

the video monitoring device comprises a camera and a video monitor, wherein the camera is fixed right above a square outer frame of the water tank through a camera support and is used for shooting a mouse load swimming or no load swimming picture in each swimming barrel of the water tank; the cameras are respectively and electrically connected with the video monitor and camera interfaces arranged on the water tank control cabinet, and the video monitor is electrically connected with a PC (personal computer) through a data line; the camera interface is electrically connected with a camera switch arranged on the water tank control cabinet, and the camera switch is electrically connected with a power supply interface arranged on the water tank control cabinet;

the water circulating pump is arranged in the lower interval of the inner cavity of the square outer frame of the water tank and is electrically connected with a water circulating pump switch arranged on the water tank control cabinet, and the water circulating pump switch is electrically connected with a power interface arranged on the water tank control cabinet.

In the technical scheme, the square inner partition plate with holes, the square inner grid frame and the N swimming cylinders are detachably arranged in the inner cavity of the square outer frame;

the water tank control cabinet is arranged on the outer frame wall of the square outer frame of the water tank or independently arranged outside the water tank.

In the technical scheme, a plurality of dense through holes are uniformly distributed on the square inner partition plate with holes, and the aperture of each through hole is smaller than the grain size of mouse excrement.

In the technical scheme, the upper part of one outer side surface of the square outer frame is also provided with a water inlet pipe, and the lower part of the outer side surface of the square outer frame is also provided with a water outlet pipe;

the water inlet pipe is also provided with a manual water inlet valve, and the water outlet pipe is also provided with a manual water outlet valve; the water tank is communicated with an external tap water pipe through the water inlet pipe and is communicated with an external drainage groove through a drainage pipe.

In the technical scheme, the upper top surface of each swimming barrel is flush with the upper top surfaces of the square outer frame and the square inner grid frame; the lower top surface of each swimming cylinder is flush with the lower top surface of the square inner grid frame and is attached to the upper top surface of the square inner partition plate with holes.

In the technical scheme, each digital display timer comprises a timing digital display screen, and a timing start button, a timing end button and a timing reset button which are arranged on one side of the timing digital display screen; every digital display timer all contains count down digital display screen) and set up and be in count down start button A, count down end button A, function adjustment key A and the setting of count down digital display screen one side are in count down start button B, count down end button B, the function setting key B of count down digital display screen opposite side.

Among the above-mentioned technical scheme, still set firmly four universal caster and four can convertible supporting legs on the outer diapire of square frame.

In the technical scheme, the height of each swimming barrel is 400-500 mm, and the swimming barrels are made of organic glass; the square outer frame is a square frame with an upper end and an outer flanging opening, a flanging position on the rear side of the upper end of the square outer frame and a flanging position on the front side of the upper end of the square outer frame are respectively provided with a row of labels, wherein the row of labels on the flanging position on the rear side of the upper end of the square outer frame correspond to a plurality of swimming barrels on the rear row of the square outer frame one to one, and the row of labels on the flanging position on the front side of the upper end of the square outer frame correspond to a plurality of swimming barrels on the front row of the square outer frame one to one.

A method of performing a weight-bearing swim test of a mouse using a device suitable for evaluating a fatigue-alleviating functional test of the mouse, comprising the steps of:

s11, filling a water supply tank with a proper amount of tap water required by the mouse weight swimming test;

s12, switching on a power supply, pressing a temperature control switch on a water tank control cabinet, starting a heating temperature control device to work, then pressing a heating button on a temperature digital display regulator, starting an electric heater, heating tap water filled into the water tank through the electric heater in the heating temperature control device, monitoring the change of the water temperature in the water tank in real time through a water temperature sensor in the heating temperature control device, transmitting a water temperature signal monitored by the water temperature sensor to the temperature digital display regulator in the heating temperature control device, and displaying the numerical value of the water temperature signal monitored by the water temperature sensor through the temperature digital display regulator;

s13, when an operator observes that the water temperature value displayed on the temperature digital display regulator reaches the preset temperature required by the mouse weight swimming test, sequentially pressing a camera switch on a water tank control cabinet, starting a video monitoring device to work, pressing a water circulating pump switch on the water tank control cabinet, starting a water circulating pump to work, then sequentially placing an experimental group mouse or a control group mouse in each swimming barrel of the water tank, and pressing a timing start button on a digital display timer in the timing device corresponding to the swimming barrel at the moment that the mouse is placed in each swimming barrel to start the mouse weight swimming timing, wherein the initial moment when the timing start button on the digital display timer in each timing device is pressed is 0 minute corresponding to the initial moment displayed on the digital timing display screen: 0 second;

s14, after a timing start button on a digital display timer in each timing device is pressed, an operator can observe the weight swimming state of each mouse in the corresponding swimming barrel through a video monitor in the video monitoring device until the mouse dies due to exhaustion;

when observing that a mouse in a certain swimming barrel dies, an operator needs to immediately press a timing end button on a digital display timer in a timing device corresponding to the swimming barrel, record the death time of the mouse when the weight swimming force of the mouse is exhausted, and take out the dead mouse from the swimming barrel;

the temperature control device is used for monitoring the change of the water temperature in the water tank in real time in the whole process of mouse weight swimming, and if the water temperature in the water tank is lower than the preset temperature required by a mouse weight swimming test, a heating button on the temperature digital display regulator can be manually pressed or automatically regulated by the temperature digital display regulator, so that the electric heater is started to work; if the water temperature in the water tank is higher than the preset temperature required by the mouse weight swimming test, a pause button on the temperature digital display regulator can be manually pressed or automatically regulated by the temperature digital display regulator, so that the electric heater is paused;

s15, transmitting the exhaustion death time of the mouse weight-bearing swimming recorded by the digital display timer in each timing device to a PC (personal computer) through a data line, calculating the weight-bearing swimming time of the mouse in an EXCEL table form by the PC, and counting the number of the weight-bearing swimming time of the mouse within 30 minutes;

s16, after each group of tests is completed, sequentially pressing a timing reset button on a digital display timer in each timing device, resetting data displayed on a timing digital display screen in each timing device, sequentially pressing a temperature control switch, a water circulating pump switch and a camera switch on a water tank control cabinet, and switching off a power supply to stop the heating temperature control device, the timing device, the video monitoring device and the water circulating pump;

s17, repeating the steps S11 to S16 to carry out the next experiment group or control group mouse weight swimming test or end test;

wherein, the control group of mice refers to mice which are not given special treatment, and the roots of the tails of the mice are loaded with 5 percent weight lead skin before being placed in a water tank;

experimental group mice, refer to mice given different doses of test substance and loaded with 5% weight lead skin at the root of the tail before being placed in the water tank.

A test method for measuring serum urea or blood lactic acid by swimming a mouse without load by using a device suitable for a test for evaluating the fatigue-relieving function of the mouse comprises the following steps:

s21, filling a proper amount of tap water required by the mouse non-weight bearing swimming test into a water supply tank;

s22, switching on a power supply, starting timing devices to work, pressing a function adjusting key A on a digital display timer in each timing device to set the non-weight swimming time of the mouse and pressing a function adjusting key B on the digital display timer in each timing device to set the rest time of the mouse according to the test requirements of measuring serum urea or blood lactic acid in the non-weight swimming test of the mouse;

(1) when the device suitable for evaluating the fatigue-relieving function test of the mouse is used for carrying out the serum urea test for the swimming without load, the initial time when the countdown starting button A on the digital display timer in each timing device is pressed is set as the duration of the swimming without load required by the serum urea test of the mouse, the initial time when the countdown starting button B on the digital display timer in each timing device is pressed is set as the rest duration required by the serum urea test of the mouse, and then the step S23 is carried out;

(2) when the device suitable for evaluating the fatigue-relieving function test of the mouse is used for carrying out the blood lactic acid test of swimming without bearing a load, the initial time when the countdown starting button A on the digital display timer in each timing device is pressed is set as the swimming time length without bearing a load required by the blood lactic acid test of the mouse, the initial time when the countdown starting button B on the digital display timer in each timing device is pressed is set as the rest time length required by the blood lactic acid test of the mouse, and then the step S23 is carried out;

s23, pressing a heating button on the digital temperature display regulator, starting an electric heater to work, heating tap water filled into the water tank through the electric heater in the heating temperature control device, monitoring the change of water temperature in the water tank in real time through a water temperature sensor in the heating temperature control device, transmitting a water temperature signal monitored by the water temperature sensor to the digital temperature display regulator in the heating temperature control device, and displaying the numerical value of the water temperature signal monitored by the water temperature sensor through the digital temperature display regulator;

s24, when an operator observes that the water temperature value displayed on the temperature digital display regulator reaches a preset temperature required by a test for measuring serum urea or blood lactic acid by swimming without loading a mouse, sequentially pressing a camera switch on the water tank control cabinet, starting the video monitoring device to work, pressing a water circulating pump switch on the water tank control cabinet, and starting the water circulating pump to work;

(1) when a device suitable for evaluating the fatigue-relieving function test of the mice is used for carrying out the serum urea test of swimming without bearing a load, the next operation is carried out according to S25;

(2) when a device suitable for evaluating the fatigue-relieving function test of the mouse is adopted to carry out the blood lactic acid test of swimming without bearing a load, the next operation is carried out according to S26;

s25, keeping the current water temperature of the water tank at a preset temperature required by a serum urea test for the mouse swimming without load, then sequentially placing a mouse of an experimental group or a mouse of a control group into each swimming cylinder of the water tank, and pressing down a countdown starting button A on a digital display timer in a timing device corresponding to each swimming cylinder at the moment that the mouse is placed into each swimming cylinder to start the countdown of the mouse swimming without load;

(1) when a warning lamp A on a certain timing device begins to flicker and a buzzer A begins to sound, the fact that a mouse in a swimming cylinder corresponding to the timing device does not bear a load and swim for a certain time is indicated, a countdown ending button A on a digital display timer in the timing device needs to be immediately pressed down, the warning lamp A is turned off, the buzzer A stops sounding, and then the mouse in the swimming cylinder is fished out and placed in a designated rest area; when the mouse is fished out from the corresponding swimming barrel, a countdown starting button B on a digital display timer in the timing device corresponding to the mouse needs to be immediately pressed, and the mouse rest countdown is started;

(2) when a warning lamp B on a certain timing device begins to flicker and a buzzer B begins to sound, a mouse in a swimming barrel corresponding to the timing device is shown, the mouse is taken out from a water tank to a specified rest area, the rest time is up, a countdown ending button B on a display timer in the timing device needs to be immediately pressed down, the warning lamp B is turned off, the buzzer B stops sounding, then 0.5mL (without anticoagulant) of blood is collected from the tail root of the mouse, the mouse is placed in a refrigerator at 4 ℃ for about 3 hours, a centrifuge is adopted to centrifuge at the rotating speed of 2000prn/min for 15min after blood coagulation, serum is taken for standby, and finally the urea content in the serum is measured by using a diacetyl-oxime method;

the temperature control device is used for monitoring the change of the water temperature in the water tank in real time in the whole process of the mouse swimming without bearing a load, and if the water temperature in the water tank is lower than the preset temperature required by the serum urea test for the mouse swimming without bearing a load, a heating button on the temperature digital display regulator can be manually pressed or the temperature digital display regulator can automatically regulate the temperature digital display regulator, so that the electric heater is started to work; if the water temperature in the water tank is higher than the preset temperature required by the serum urea test for swimming without load of the mouse, a pause button on the temperature digital display regulator can be manually pressed or automatically regulated by the temperature digital display regulator, so that the electric heater is paused;

s26, keeping the current water temperature of the water tank at a preset temperature required by a blood lactic acid test for the non-weight bearing swimming of mice, sequentially placing a mouse of an experimental group or a mouse of a control group into each swimming cylinder of the water tank, and pressing down a countdown starting button A on a display timer in a timing device corresponding to each swimming cylinder at the moment that the mouse is placed into each swimming cylinder to start the countdown of the non-weight bearing swimming of the mice; wherein, before each mouse is placed into the corresponding swimming cylinder, 20 mu L of blood is collected at the tail root of each mouse, then 40 mu L of the membrane breaking liquid is added, and the cells are immediately and fully vibrated and broken to finish the first blood collection;

(1) when a warning lamp A on a certain timing device begins to flash and a buzzer A begins to sound, the fact that a mouse in a swimming barrel corresponding to the timing device does not bear a load for swimming is indicated to be reached, a countdown ending button A on a digital display timer in the timing device needs to be immediately pressed down, the warning lamp A is turned off, the buzzer A stops sounding, then the mouse in the swimming barrel is fished out and placed in a designated rest area, 20 mu L of blood is collected at the tail root of the mouse, 40 mu L of rupture fluid is added for oscillation, and secondary blood collection is completed;

(2) after the mice in each swimming barrel finish the non-weight swimming and the second blood sampling, a countdown starting button B on a digital display timer in the corresponding timing device needs to be immediately pressed, so that the mice rest in a designated rest area for the rest time preset by the system, and then the blood sampling is carried out for the third time;

(3) when a warning lamp B on a certain timing device begins to flash and a buzzer B begins to sound, a mouse in a swimming barrel corresponding to the timing device is shown, the mouse is fished out from a water tank to a specified rest area, the time of rest is up, a countdown ending button B on a digital display timer in the timing device needs to be immediately pressed, the warning lamp B is turned off, the buzzer B stops sounding, then blood is collected at the tail root of the mouse by 20 mu L, 40 mu L of membrane breaking liquid is added for oscillation, and the third blood collection is completed;

wherein, after blood is collected each time, the lactate content in the blood is measured by a lactate measuring instrument;

the temperature control device is used for monitoring the change of the water temperature in the water tank in real time in the whole process of the mouse swimming without bearing a load, and if the water temperature in the water tank is lower than the preset temperature required by the blood lactic acid test for the mouse swimming without bearing a load, a heating button on the temperature digital display regulator can be manually pressed or the temperature digital display regulator can automatically regulate the temperature digital display regulator, so that the electric heater is started to work; if the water temperature in the water tank is higher than the preset temperature required by the blood lactic acid test for swimming without load, a pause button on the temperature digital display regulator can be manually pressed or automatically regulated by the temperature digital display regulator, so that the electric heater stops working;

s27, after each group of tests is finished, sequentially pressing a temperature control switch, a water circulating pump switch and a camera switch on the water tank control cabinet, and disconnecting the power supply to stop the heating temperature control device, the timing device, the video monitoring device and the water circulating pump;

s28, repeating the steps S21-S25 and S27 to carry out the serum urea test or the ending test of the mice of the next experimental group or the control group without carrying weight swimming;

s29, repeating the steps S21-S24 and S26-S27 to carry out the blood lactic acid test or the end test of the next experimental group or the control mice without weight bearing swimming;

wherein, the control group mice refer to mice which are not given special treatment;

experimental group mice, refer to mice given different doses of the test substance.

Compared with the prior art, the utility model discloses an advantage and beneficial effect are:

1. the internal components of the water tank adopt a detachable design, so that the water tank is convenient to mount and dismount, parts are convenient to replace, the inside of the water tank is convenient to clean, the mutual interference of mice in the swimming process can be avoided, and the test accuracy of an experimental result is improved;

2. the heating temperature control device consisting of the electric heating pipe, the water temperature sensor and the temperature digital display regulator is adopted, so that the water temperature in the water tank can be ensured to be in a preset test range of the system in real time, the fluctuation influence of the external environment temperature on the water temperature in the water bucket is reduced, and the accuracy of a test structure is further ensured;

3. an independent timing device is arranged for each swimming barrel and used for independently recording the swimming time of the mouse in each swimming barrel, so that the problem of difficulty in manual timing is solved, and the experimental result is more accurate and reliable;

4. the water circulation pump is used for circulating water flow with the same temperature and the same flow speed in the swimming barrels, so that the water environment in each swimming barrel is ensured to be the same, and the mice in each swimming barrel are prevented from floating;

5. the video monitoring device arranged on the water tank is convenient for recording the video of mouse load swimming or no load swimming, the experimental state of the animal can be well and conveniently observed without the need of an operator to be on the side at any time, and the accuracy of the experimental result is favorably analyzed;

6. the device suitable for evaluating the mouse fatigue relieving function test can be used for various tests and solves the problem of single function of the existing mouse swimming box; for example: the method can be used for determining the exhaustion duration of an animal weight swimming test, can also be used for determining the content of serum urea or blood lactic acid in an animal non-weight swimming test, and can also be used for determining the content of red blood cells, hemoglobin and the like in an animal red blood cell oxygen carrying capacity test.

Drawings

FIG. 1 is a schematic view of the working principle of the present invention;

FIG. 2 is an embodiment of the present invention;

FIG. 3 is a schematic view of the structure of the water tank of FIG. 2;

FIG. 4 is an exploded view of the water tank of FIG. 3;

FIG. 5 is a top view of the tank of FIG. 3;

3 FIG. 36 3 is 3a 3 cross 3- 3 sectional 3 view 3 taken 3 along 3 line 3A 3- 3A 3 of 3 FIG. 35 3; 3

FIG. 7 is a schematic structural diagram of the water tank control cabinet in FIG. 3;

FIG. 8 is an enlarged view of the single timing device of FIG. 7;

in the figure: 1. a water tank; 1.1, a square outer frame; 1.2, a square internal clapboard with holes; 1.3, a square inner grid frame; 1.4, a swimming barrel; 1.5, a square swimming area; 1.6, a water inlet pipe; 1.7, a drain pipe; 1.8, a manual water inlet valve; 1.9, a manual drain valve; 1.10, universal caster wheels; 1.11, reversible supporting legs; 1.12, label; 2. a water tank control cabinet; 2.1, a power interface; 2.2, a temperature control switch; 2.3, switching a water circulating pump; 2.4, a camera switch; 2.5, a camera interface; 2.6, PC data interface; 2.7, a PLC programmable controller; 3. heating a temperature control device; 3.1, a water temperature sensor; 3.2, an electric heater; 3.3, a temperature digital display regulator; 3.3a, a heating button; 3.3b, pause button; 4. a timing device; 4.1, a digital display timer; 4.1a, a timing digital display screen; 4.1b, a timing start button; 4.1c, a timing end button; 4.1d, a timing reset button; 4.2, a digital display timer 4.2a and a countdown digital display screen; (ii) a4.2b, a countdown start button A; 4.2c, a countdown ending button A; 4.2d, function adjusting key A; 4.2ed, count down start button B; 4.2f, a countdown ending button B; 4.2g, function adjusting B; 4.3, a warning lamp A; 4.4, a buzzer A; 4.5, a warning light B; 4.6, a buzzer B; 5. a video monitoring device; 5.1, a camera; 5.2, video monitor; 5.3, a camera bracket; 6. a water circulation pump; 7. a PC machine.

Detailed Description

In order to make the technical means, the creation features, the achievement purposes and the functions of the present invention easy to understand and understand, how to implement the present invention is further explained below with reference to the accompanying drawings and the detailed description.

Referring to fig. 1 to 8, in order to provide an embodiment of the device for evaluating the fatigue-alleviating function test of the mouse according to the present invention, the device includes a water tank 1, a water tank control cabinet 2, a heating

temperature control device

3, 12 timing devices 4, a video monitoring device 5, a water circulation pump 6 and a PC 7; wherein:

the water tank 1 comprises a square outer frame 1.1, a square inner partition plate with holes 1.2 arranged in the square outer frame 1.1, a square inner grid frame 1.3 and 12 swimming barrels 1.4, wherein the inner cavity of the square outer frame 1.1 is divided into an upper interval and a lower interval by the square inner partition plate with holes 1.2; the square inner grid frame 1.3 is positioned in the upper section of the inner cavity of the square outer frame 1.1, the upper section of the inner cavity of the square outer frame 1.1 is divided into a plurality of square swimming areas 1.5, and a swimming barrel 1.4 is embedded in each square swimming area 1.5; the heating temperature control device 3 comprises a water temperature sensor 3.1 arranged in the lower section of the inner cavity of the square outer frame 1.1, an electric heater 3.2 and a temperature digital display regulator 3.3 arranged on the water tank control cabinet 2; the water temperature sensor 3.1 and the electric heater 3.2 are both electrically connected with a temperature digital display regulator 3.3, the temperature digital display regulator 3.3 is electrically connected with a temperature control switch 2.2 arranged on the water tank control cabinet 2, the temperature control switch 2.2 is electrically connected with a power interface 2.1 arranged on the water tank control cabinet 2, and the power interface 2.1 is electrically connected with an external power supply through a power line;

12 timing devices 4 are arranged on the water tank control cabinet 2, each timing device 4 is matched and corresponding to one swimming barrel 1.4 in the water tank 1 and is electrically connected with a PLC (programmable logic controller) 2.7 arranged in the water tank control cabinet 2, each timing device 4 comprises a digital display timer 4.1, a digital display timer 4.2, a warning lamp A4.3, a warning lamp B4.5, a buzzer A4.4 and a buzzer B4.6, and the warning lamp A4, the warning lamp B4.5, the buzzer A4.4 and the buzzer B4.6 in each timing device 4 are electrically connected with the corresponding digital display timer 4.2; the PLC 2.7 is electrically connected with the PC 7 through a PC data interface 2.6 arranged on the water tank control cabinet 2;

the video monitoring device 5 comprises a camera 5.1 and a video monitor 5.2, wherein the camera 5.1 is fixed right above a square outer frame 1.1 of the water tank 1 through a camera support 5.3 and is used for shooting a mouse weight swimming or non-weight swimming picture in each swimming bucket 1.4 of the water tank 1; the camera 5.1 is respectively and electrically connected with a video monitor 5.2 and a camera interface 2.5 arranged on the water tank control cabinet 2, and the video monitor 5.2 is electrically connected with the PC 7 through a data line; the camera interface 2.5 is electrically connected with a camera switch 2.4 arranged on the water tank control cabinet 2, and the camera switch 2.4 is electrically connected with the power interface 2.1;

the water circulating pump 6 is arranged in the lower interval of the inner cavity of the square outer frame 1.1 of the water tank 1 and is electrically connected with a water circulating pump switch 2.3 arranged on the water tank control cabinet 2, and the water circulating pump switch 2.3 is electrically connected with a power supply interface 2.1.

In order to facilitate the cleaning of the inner side of the water tank and the replacement of parts, in the embodiment of the utility model, the square inner clapboard with holes 1.2, the square inner grid frame 1.3 and a plurality of swimming cylinders 1.4 are all detachably arranged in the inner cavity of the square outer frame 1.1; when the water tank inner lining needs to be cleaned, the square inner partition plate with holes 1.2, the square inner grid frame 1.3 and the swimming barrels 1.4 arranged in the water tank inner lining are firstly drawn out, then the water tank inner lining is cleaned, and after the cleaning is finished, the square inner partition plate with holes 1.2, the square inner grid frame 1.3 and the swimming barrels 1.4 are placed back into the water tank inner lining again.

According to actual design needs, water tank switch board 2 can set up on the frame wall of 1 square frame 1.1 of water tank or independently set up in 1 outsides of water tank the embodiment of the utility model provides an, water tank switch board 2 sets up on the front side frame wall of square frame 1.1.

In order to prevent a mouse from contacting with an electric heater 3.2 and a water circulating pump 6 in the water tank 1, the inner cavity of the water tank 1 is divided into an upper interval and a lower interval by a square internal partition plate 1.2 with holes, wherein the upper interval of the inner cavity of the water tank 1 is used as a swimming area of the mouse, and the lower interval of the inner cavity of the water tank 1 is used as a water temperature control area of the water tank 1.

In order to enable water in a water temperature control area at the lower part of the water tank to flow into a swimming area of a mouse at the upper part, a plurality of dense through holes are uniformly distributed on a square inner partition plate 1.2 with holes; in addition, in order to prevent the excrement discharged by the mouse during the swimming process from sinking into the bottom of the water tank to pollute the electric heater and the water circulating pump, the aperture of each through hole is designed to be smaller than the grain diameter of the excrement of the mouse.

In the embodiment of the utility model, a water inlet pipe 1.6 is arranged on the upper part of the left outer side surface of the square outer frame 1.1, and a water outlet pipe 1.7 is arranged on the lower part; a manual water inlet valve 1.8 is also arranged on the water inlet pipe 1.6; a manual drain valve 1.9 is also arranged on the drain pipe 1.7; the water tank 1 is communicated with an external tap water pipe through a water inlet pipe 1.6 and is communicated with an external drainage groove through a drainage pipe 1.7.

In the embodiment of the present invention, the upper top surface of each swimming barrel 1.4 is flush with the upper top surfaces of the square outer frame 1.1 and the square inner grid frame 1.3, the lower top surface of the square inner grid frame 1.3 of each swimming barrel 1.4 is flush with the lower top surface of the square inner grid frame 1.3, and the upper top surface of the square inner grid frame 1.2 is attached.

In the embodiment of the utility model, each digital display timer 4.1 comprises a timing digital display screen 4.1a, a timing start button 4.1b, a timing end button 4.1c and a timing reset button 4.1d which are arranged on one side of the timing digital display screen 4.1 a; each digital display timer 4.2 includes a count-down digital display 4.2a, and a count-down start button a4.2b, a count-down end button a4.2c, a function adjustment key a4.2d, a count-down start button b4.2e, a count-down end button b4.2f, and a function setting key b4.2g provided on one side of the count-down digital display 4.2a, and provided on the other side of the count-down digital display 4.2 a.

In order to move the water tank conveniently, in the embodiment of the utility model, four universal casters 1.12 and four reversible supporting legs 1.13 are fixedly arranged on the outer bottom wall of the square outer frame 1.1 of the water tank 1; when needs remove the water tank, earlier four can convertible supporting leg 1.13 upturns of water tank bottom, undertakes the weight of water tank by four universal castor 1.12, then shifts the water tank to the required position of work as required, treats that the water tank removes behind the required position of work, can convertible supporting leg 1.13 with four of the upturning of water tank bottom puts down, undertakes the weight of water tank by it, and universal castor 1.12 does not undertake weight.

In order to prevent the mouse from climbing out of the water tank along the wall of the swimming barrel, in the embodiment of the utility model, the section of each swimming barrel 1.4 is round, and the height is selected within the range of 400 mm-500 mm according to the selection and is made of organic glass; the preferred height of each swimming bucket is 450 mm; the purpose of using organic glass for the material of every swimming bucket is to prevent that its use from taking place wearing and tearing, influencing its life.

In order to facilitate an observer to visually distinguish each swimming barrel, the square outer frame 1.1 is a square frame with an opening at the periphery of an outer flanging at the upper end, a flanging part at the rear side of the upper end of the square outer frame 1.1 and a flanging part at the front side of the upper end of the square outer frame 1.1 are respectively provided with a row of labels 1.12, wherein a row of labels 1.12 at the flanging part at the rear side of the upper end of the square outer frame 1.1 correspond to 6 swimming barrels 1.4 at the rear row of the square outer frame 1.1 one by one, and a row of labels 1.12 at the flanging part at the front side of the upper end of the square outer frame 1.1 correspond to 6 swimming barrels 1.4 at the front row of the square outer frame 1.1 one by one; the label 1.12 can be a label printed with a number or a letter number according to actual needs.

The utility model provides a be suitable for evaluation mouse and alleviate device of fatigue function test, it is long when both can be used to mouse heavy burden swimming test survey mouse power exhaustion, also can be used to the non-heavy burden swimming test survey serum urea of mouse or blood lactic acid content, can also be used to mouse red blood cell and take oxygen capacity test survey red blood cell, hemoglobin content etc.

(one) when adopting the utility model provides a be suitable for the experimental device of evaluation mouse fatigue-alleviating function to carry out mouse heavy burden swimming test, it specifically contains following step:

s11, filling a proper amount of tap water required by a mouse weight swimming test into the water supply tank 1;

s12, a power supply is switched on, a temperature control switch 2.2 on a water tank control cabinet 2 is pressed, a heating temperature control device 3 is started to work, then a heating button 3.3a on a temperature digital display regulator 3.3 is pressed, an electric heater 3.2 is started to work, tap water filled into a water tank 1 is heated by the electric heater 3.2 in the heating temperature control device 3, the water temperature change in the water tank 1 is monitored in real time by a water temperature sensor 3.1 in the heating temperature control device 3, a monitored water temperature signal is transmitted to the temperature digital display regulator 3.3 in the heating temperature control device 3, and the digital display regulator 3.3 displays the water temperature signal value monitored by the water temperature sensor 2.1;

s13, when the operator observes that the water temperature value displayed on the digital temperature display regulator 3.3 reaches the preset temperature required by the mouse weight swimming test and is 25 ℃, the camera switch 2.4 on the water tank control cabinet 2 is pressed down in sequence, the video monitoring device 5 is started to work, the water circulating pump switch 2.3 on the water tank control cabinet 2 is pressed down, the water circulating pump 6 is started to work, then, one experimental group mouse or one control group mouse is respectively put in each swimming barrel 1.4 of the water tank 1 in turn, and a timing start button 4.1b on a digital display timer 4.1 in a timing device 4 corresponding to each swimming bucket 1.4 is pressed at the moment that the mouse is put into the swimming bucket 1.4, the mouse weight swimming timing is started, wherein, the initial time when the timing start button 4.1b on each digital display timer 4.1 is pressed is 0 minute corresponding to the initial time displayed on the timing digital display screen 4.1 a: 0 second;

s14, after the timing start button 4.1a of the digital display timer 4.1 is pressed down in each timing device 4, the operator can observe the weight swimming state of each mouse in the corresponding swimming bucket 1.4 through the video monitor 5.2 in the video monitoring device 5 until the mouse dies due to exhaustion;

when an operator observes that a mouse in a certain swimming barrel 1.4 dies, the operator immediately presses a timing end button 4.1c on a digital display timer 4.1 in a timing device 4 corresponding to the swimming barrel 1.4 to record the dead time of the mouse when the load swimming capability of the mouse is exhausted, and the dead mouse is fished out of the swimming barrel 1.4;

wherein, in the whole process of mouse load swimming, the water temperature change in the water tank 1 is monitored in real time by the heating temperature control device 2, if the water temperature in the water tank 1 is lower than the preset temperature 25 ℃ required by the mouse load swimming test, a heating button 3.3a on the temperature digital display regulator 3.3 can be manually pressed or automatically regulated by the temperature digital display regulator 3.3, and the electric heater 3.2 is started to work; if the water temperature in the water tank 1 is higher than the preset temperature 25 ℃ required by the mouse weight swimming test, a pause button 3.3b on the digital temperature display regulator 3.3 can be manually pressed or automatically regulated by the digital temperature display regulator 3.3, so that the electric heater 3.2 is paused;

s15, the mouse weight swimming exhaustion death time recorded by the digital display timer 4.1 in each timing device 4 is transmitted to the PC 7 through a data line, the PC 7 calculates the mouse weight swimming time in an EXCEL table form, and the quantity of the mouse weight swimming time within 30 minutes is counted;

s16, after each group of tests is finished, sequentially pressing a timing reset button 4.1d on a digital display timer 4.1 in each timing device 4, resetting data displayed on a timing digital display screen 4.1a in each timing device 4, sequentially pressing a temperature control switch 2.2, a water circulating pump switch 2.3 and a camera switch 2.4 on the water tank control cabinet 2, and switching off a power supply to stop the heating temperature control device 3, the timing device 4, the video monitoring device 5 and the water circulating pump 6 from working;

(II) when adopting the utility model provides a be suitable for the experimental device of evaluation mouse fatigue-alleviating function to carry out the experimental serum urea of mouse non-heavy burden swimming survey or blood lactic acid, it specifically contains following step:

s21, filling a proper amount of tap water required by the mouse non-load swimming test into the water supply tank 1;

s22, switching on a power supply, starting the timing device 4 to work, and according to the test requirement of determining serum urea or blood lactic acid in the mouse non-load swimming test, pressing a function adjusting key A4.2d on a digital display timer 4.2 in each timing device 4 to set the mouse non-load time and pressing a function adjusting key B4.2g on each digital display timer 4.2 to set the mouse rest time;

(1) when the device suitable for evaluating the fatigue-relieving function test of the mouse is used for carrying out the serum urea test for swimming without bearing a load, the initial time when the countdown starting button A4.2b on the display timer 4.2 in each timing device 4 is pressed is set as the time length of swimming without bearing a load required by the serum urea test of the mouse, the initial time when the countdown starting button B4.2e on the display timer 4.2 in each timing device 4 is pressed is set as the time length of rest required by the serum urea test of the mouse, and then the step S23 is carried out;

(2) when a device suitable for evaluating a mouse fatigue-relieving function test is used for a mouse swimming test for measuring blood lactic acid without load, the initial time when the countdown starting button A4.2b on the count-up timer 4.2 in each timing device 4 is pressed is set as the swimming time without load required by the mouse blood lactic acid measurement test, the initial time when the countdown starting button B4.2e on the count-up timer 4.2 in each timing device 4 is pressed is set as the rest time required by the mouse blood lactic acid measurement test, and then the process goes to step S23;

s23, pressing a heating button 3.3a on a temperature digital display regulator 3.3, starting an electric heater 3.2 to work, heating tap water filled into a water tank 1 through the electric heater 3.2 in a heating temperature control device 3, monitoring the change of the water temperature in the water tank 1 in real time through a water temperature sensor 3.1 in the heating temperature control device 3, transmitting a monitored water temperature signal to the temperature digital display regulator 3.3 in the heating temperature control device 3, and displaying the numerical value of the water temperature signal monitored by the water temperature sensor 3.1 through the temperature digital display regulator 3.3;

s24, when an operator observes that the water temperature numerical value displayed on the digital temperature display regulator 3.3 reaches the preset temperature required by the test for determining serum urea or blood lactic acid by swimming without loading a load, and when the temperature reaches 30 ℃, a pause button 3.3b on the digital temperature display regulator 3.3 is pressed to pause the electric heater 3.2, then a camera switch 2.4 on the water tank control cabinet 2 is sequentially pressed, the video monitoring device 5 is started to work, a water circulating pump switch 2.3 of the water tank control cabinet 2 is pressed, and the water circulating pump 6 is started to work;

s25, keeping the current water temperature of the water tank 1 at a preset temperature of 30 ℃ required by the mouse non-weight swimming measurement serum urea, then sequentially placing a mouse of an experimental group or a mouse of a control group in each swimming cylinder 1.4 of the water tank 1, and pressing down a countdown starting button A4.2b on a count-up timer 4.2 of a timing device 4 corresponding to the swimming cylinder 1.4 at the moment that the mouse is placed in each swimming cylinder 1.4 to start the countdown of the mouse non-weight swimming;

(1) when a warning lamp A4.3 on a certain timing device 4 begins to flash and a buzzer A4.4 begins to sound, the fact that a mouse in a swimming barrel 1.4 corresponding to the timing device 4 is not loaded with weight and swims for a certain time is shown, a countdown ending button A4.2d on a timer 4.2 in the timing device 4 is immediately pressed, the warning lamp A4.3 is turned off, the buzzer A4.4 stops sounding, and then the mouse in the swimming barrel 1.4 is fished out and placed in a designated rest area; when the mouse is fished out from the corresponding swimming cylinder 1.4, a countdown starting button B4.2e on a count-up timer 4.2 in the timing device 4 corresponding to the mouse needs to be immediately pressed, and the mouse rest countdown is started;

(2) when a warning lamp B4.5 on a certain timing device 4 begins to flash and a buzzer B4.6 begins to sound, a mouse in a swimming barrel 1.4 corresponding to the timing device 4 is indicated, when the mouse is fished out from a water tank 1 to a specified rest area, the rest time is up, a countdown ending button B4.2f on a display timer 4.2 in the timing device 4 is immediately pressed, the warning lamp B4.5 is extinguished, the buzzer B4.6 stops sounding, then 0.5mL (without anticoagulant) of blood is collected from the tail root of the mouse, the mouse is placed in a refrigerator at 4 ℃ for about 3 hours, a centrifuge is adopted to centrifuge at the rotating speed of 2000prn/min for 15min after blood coagulation, serum is taken for standby, and finally the content of urea in the serum is measured by a diacetyl-oxime method;

wherein, in the whole process of the mouse swimming without bearing a load, the water temperature change in the water tank 1 is monitored in real time by the heating temperature control device 2, if the water temperature in the water tank 1 is lower than the preset temperature of 30 ℃ required by the serum urea test for the mouse swimming without bearing a load, the heating button 3.3a on the temperature digital display regulator 3.3 can be manually pressed or automatically regulated by the temperature digital display regulator 3.3, and the electric heater 3.2 is started to work; if the water temperature in the water tank 1 is 30 ℃ higher than the preset temperature required by the test for determining the serum urea by swimming without load of the mice, a pause button 3.3b on the temperature digital display regulator 3.3 can be manually pressed or automatically regulated by the temperature digital display regulator 3.3, so that the electric heater 3.2 is paused;

s26, keeping the current water temperature of the water tank 1 at a preset temperature of 30 ℃ required by a blood lactic acid test for the non-weight bearing swimming of mice, sequentially placing a mouse of an experimental group or a mouse of a control group in each swimming cylinder 1.4 of the water tank 1, and pressing a countdown starting button A4.2b on a count-up timer 4.2 of a timing device 4 corresponding to each swimming cylinder 1.4 at the moment that the mouse is placed in each swimming cylinder 1.4 to start the countdown of the non-weight bearing swimming of the mice; wherein, before each mouse is placed into the corresponding swimming cylinder 1.4, 20 mu L of blood is collected at the tail root of each mouse, then 40 mu L of the membrane breaking liquid is added, and the cells are immediately and fully vibrated and broken to finish the first blood collection;

(1) when the warning lamp A4.3 on a certain timing device 4 begins to flash and the buzzer A4.4 begins to sound, the fact that the mouse in the swimming barrel 1.4 corresponding to the timing device 4 has no weight bearing swimming time is up is indicated, a countdown ending button A4.2c on the timing device 4 needs to be pressed immediately, the warning lamp A4.3 is turned off, the buzzer A4.4 stops sounding, then the mouse in the swimming barrel 1.4 is fished out and placed in a specified rest area, 20 muL of blood is collected at the tail root of the mouse, 40 muL of rupture of membranes is added to oscillate, and secondary blood collection is completed;

(2) after the mice in each swimming barrel 1.4 finish swimming without load and secondary blood sampling, a countdown starting button B4.2e on a digital display timer 4.2 in the corresponding timing device 4 needs to be immediately pressed, so that the mice rest in a designated rest area for the rest time preset by the system, and then blood sampling is carried out for the third time;

(3) when a warning lamp B4.5 on a certain timing device 4 begins to flash and a buzzer B4.6 begins to sound, a mouse in a swimming barrel 1.4 corresponding to the timing device 4 is shown, when the mouse is fished out from a water tank 1 to a specified rest area, the mouse needs to immediately press a countdown ending button B4.2e on a digital display timer 4.2 in the timing device 4 to extinguish the warning lamp B4.5 and stop the sound of the buzzer B4.6, then 20 mu L of blood is collected from the tail root of the mouse, 40 mu L of breaking membrane liquid is added for oscillation, and the third blood collection is completed;

wherein, in the whole process of the mouse swimming without bearing a load, the water temperature change in the water tank 1 is monitored in real time by the heating temperature control device 2, if the water temperature in the water tank 1 is lower than the preset temperature of 30 ℃ required by the blood lactic acid test for the mouse swimming without bearing a load, the heating button 3.3a on the temperature digital display regulator 3.3 can be manually pressed or the temperature digital display regulator 3.3 automatically regulates, and the electric heater 3.2 is started to work; if the water temperature in the water tank 1 is 30 ℃ higher than the preset temperature required by the test for measuring the blood lactic acid in the swimming without bearing a load, a pause button 3.3b on the temperature digital display regulator 3.3 can be manually pressed or automatically regulated by the temperature digital display regulator 3.3, so that the electric heater 3.2 is paused;

s27, after each group of tests is finished, sequentially pressing the temperature control switch 2.12, the water circulating pump switch 2.3 and the camera switch 2.4 on the water tank control cabinet 2, and disconnecting the power supply to stop the heating temperature control device 3, the timing device 4, the video monitoring device 5 and the water circulating pump 6 from working;

(III) when adopting the utility model provides a be suitable for the experimental device of evaluation mouse fatigue-alleviating function to carry out the mouse erythrocyte and take oxygen capacity test, it specifically contains following step:

s31, filling a proper amount of tap water required by a mouse weight swimming test into the water supply tank 1;

s32, switching on a power supply, starting the timing device 4 to work, and pressing a function adjusting key A4.2d on a digital display timer 4.2 in each timing device 4 to set the weight-bearing swimming time of the mouse according to the oxygen carrying capacity test requirement of the mouse red blood cell, namely setting the initial time when a countdown starting button A4.2b on the digital display timer 4.2 in each timing device 4 is pressed as the swimming time required by the weight-bearing swimming of the mouse in the oxygen carrying capacity test of the mouse red blood cell for 10 min;

s33, pressing a heating button 3.3a on a temperature digital display regulator 3.3, starting an electric heater 3.2 to work, heating tap water filled into a water tank 1 through the electric heater 3.2 in a heating temperature control device 3, monitoring the change of the water temperature in the water tank 1 in real time through a water temperature sensor 3.1 in the heating temperature control device 3, transmitting a monitored water temperature signal to the temperature digital display regulator 3.3 in the heating temperature control device 3, and displaying the numerical value of the water temperature signal monitored by the water temperature sensor 3.1 through the temperature digital display regulator 3.3;

s34, when an operator observes that the water temperature value displayed on the digital temperature display regulator 3.3 reaches the preset temperature required by the load swimming of the mouse erythrocyte oxygen carrying capacity test, and the temperature is 30 ℃, sequentially pressing down the camera switch 2.4 on the water tank control cabinet 2, starting the video monitoring device 5 to work, pressing down the water circulating pump switch 2.3 on the water tank control cabinet 2, starting the water circulating pump 6 to work, then sequentially putting a test group mouse or a control group mouse into each swimming barrel 1.4 of the water tank 1, and pressing down the countdown starting button A4.2b on the digital display timer 4.1 of the timing device 4 corresponding to the swimming barrel 1.4 at the moment that each swimming barrel 1.4 is put in the mouse, so as to start the load swimming timing of the mouse;

wherein, when a mouse swims in the water tank 1 under load, the water temperature change in the water tank 1 is monitored in real time through the heating temperature control device 2, if the water temperature in the water tank 1 is lower than the preset temperature of 30 ℃ required by a blood lactic acid test for measuring the swimming without load of the mouse, a heating button 3.3a on the temperature digital display regulator 3.3 can be manually pressed or automatically regulated by the temperature digital display regulator 3.3, and the electric heater 3.2 is started to work; if the water temperature in the water tank 1 is 30 ℃ higher than the preset temperature required by the test for measuring the blood lactic acid in the swimming without bearing a load, a pause button 3.3b on the temperature digital display regulator 3.3 can be manually pressed or automatically regulated by the temperature digital display regulator 3.3, so that the electric heater 3.2 is paused;

s35, when the warning lamp A4.3 on a certain timing device 4 begins to flash and the buzzer A4.4 begins to sound, the time that the mouse in the swimming barrel 1.4 corresponding to the timing device 4 bears the weight for swimming is up, the countdown ending button A4.2d on the display timer 4.2 in the timing device 4 needs to be pressed immediately, the warning lamp A4.3 is turned off, the buzzer A4.4 stops sounding, and then the mouse in the swimming barrel 1.4 is fished out and placed in a designated rest area;

s36, immediately taking out the mouse from the swimming cylinder 1.4 and placing the mouse in a designated rest area, taking blood from the eyeball, and separating serum for later use;

s37, placing the mouse eyeball serum separated in the step S36 into an automatic blood cell counter, and measuring a red blood cell count (RBC), a packed cell volume (Hct) and hemoglobin (Hb);

s38, after each group of tests is finished, sequentially pressing the temperature control switch 2.12, the water circulating pump switch 2.3 and the camera switch 2.4 on the water tank control cabinet 2, and disconnecting the power supply to stop the heating temperature control device 3, the timing device 4, the video monitoring device 5 and the water circulating pump 6 from working;

s39, repeating the steps S31 to S39 to carry out the test of the oxygen carrying capacity of the red blood cells of the mice of the next test group or the control group or finish the test;

wherein, the control group mice, after the belly of the mice is dehaired and contacted with the control cloth for a certain time, are loaded with 5 percent weight lead skin at the root of the tail before being put into the water tank;

the experimental group of mice refers to mice with the abdomen shaved and contacted with the tested cloth for a certain time, and the roots of the tails of the mice are loaded with 5 percent weight lead skin before being placed in a water tank.

Finally, the above description is only the embodiments of the present invention, not limiting the scope of the present invention, all the equivalent structures or equivalent processes that are used in the specification and the attached drawings or directly or indirectly applied to other related technical fields are included in the patent protection scope of the present invention.

Claims

1. The utility model provides a be suitable for evaluation mouse and alleviate experimental device of fatigue function which characterized in that: comprises a water tank (1), a water tank control cabinet (2), a heating temperature control device (3), N timing devices (4), a video monitoring device (5), a water circulating pump (6) and a PC (personal computer) (7);

the water tank (1) comprises a square outer frame (1.1), and a square inner partition plate with holes (1.2), a square inner grid frame (1.3) and N swimming barrels (1.4) which are arranged in the square outer frame (1.1), wherein the inner cavity of the square outer frame (1.1) is divided into an upper interval and a lower interval by the square inner partition plate with holes (1.2); the square inner grid frame (1.3) is positioned in an upper section of the inner cavity of the square outer frame (1.1), the upper section of the inner cavity of the square outer frame (1.1) is divided into N square swimming areas (1.5), and a swimming barrel (1.4) is embedded in each square swimming area (1.5);

the heating temperature control device (3) comprises a water temperature sensor (3.1) arranged in the lower section of the inner cavity of the square outer frame (1.1), an electric heater (3.2) and a temperature digital display regulator (3.3) arranged on the water tank control cabinet (2); the water temperature sensor (3.1) and the electric heater (3.2) are electrically connected with the digital temperature display regulator (3.3), the digital temperature display regulator (3.3) is electrically connected with a temperature control switch (2.2) arranged on the water tank control cabinet (2), the temperature control switch (2.2) is electrically connected with a power supply interface (2.1) arranged on the water tank control cabinet (2), and the power supply interface (2.1) is electrically connected with an external power supply through a power line;

the water tank control cabinet is characterized in that the N timing devices (4) are arranged on the water tank control cabinet (2), each timing device (4) is matched and corresponding to one swimming barrel (1.4) in the water tank (1) and is electrically connected with a PLC (programmable logic controller) (2.7) arranged in the water tank control cabinet (2), each timing device (4) comprises a digital display timer (4.1), a digital display timer (4.2), a warning lamp A (4.3), a warning lamp B (4.5), a buzzer A (4.4) and a buzzer B (4.6), and the warning lamp A (4.3), the warning lamp B (4.5), the buzzer A (4.4) and the buzzer B (4.6) in each timing device (4) are electrically connected with the corresponding digital display timer (4.2); the PLC (2.7) is electrically connected with the PC (7) through a PC data interface (2.6) arranged on the water tank control cabinet (2);

the video monitoring device (5) comprises a camera (5.1) and a video monitor (5.2), wherein the camera (5.1) is fixed right above the water tank (1) through a camera support (5.3) and is used for shooting a mouse weight swimming or non-weight swimming picture in each swimming barrel (1.4) in the water tank (1); the camera (5.1) is respectively and electrically connected with the video monitor (5.2) and a camera interface (2.5) arranged on the water tank control cabinet (2), and the video monitor (5.2) is electrically connected with a PC (7) through a data line; the camera interface (2.5) is electrically connected with a camera switch (2.4) arranged on the water tank control cabinet (2), and the camera switch (2.4) is electrically connected with a power supply interface (2.1) arranged on the water tank control cabinet (2);

the water circulation pump (6) is arranged in the lower interval of the inner cavity of the square outer frame (1.1) of the water tank (1) and is electrically connected with a water circulation pump switch (2.3) arranged on the water tank control cabinet (2), and the water circulation pump switch (2.3) is electrically connected with a power supply interface (2.1) arranged on the water tank control cabinet (2).

2. The device for evaluating the test for the fatigue-relieving function of the mouse according to claim 1, wherein: the square inner partition plate (1.2) with holes, the square inner grid frame (1.3) and the N swimming barrels (1.4) are detachably arranged in the inner cavity of the square outer frame (1.1);

the water tank control cabinet (2) is arranged on the outer frame wall of the square outer frame (1.1) of the water tank (1) or independently arranged outside the water tank (1).

3. The device for evaluating the test for the fatigue-relieving function of the mouse according to claim 2, wherein: a plurality of dense through holes are distributed on the square inner clapboard with holes (1.2), and the aperture of each through hole is smaller than the grain diameter of the mouse excrement.

4. The device for evaluating the test for the fatigue-relieving function of the mouse according to claim 1, wherein: the upper part of one outer side surface of the square outer frame (1.1) is also provided with a water inlet pipe (1.6), and the lower part is also provided with a water outlet pipe (1.7);

a manual water inlet valve (1.8) is also arranged on the water inlet pipe (1.6), and a manual drain valve (1.9) is also arranged on the drain pipe (1.7);

the water tank (1) is communicated with an external tap water pipe through the water inlet pipe (1.6) and is communicated with an external drainage groove through the drainage pipe (1.7).

5. The device for evaluating the test for the fatigue-relieving function of the mouse according to claim 1, wherein:

the upper top surface of each swimming cylinder (1.4) is flush with the upper top surfaces of the square outer frame (1.1) and the square inner grid frame (1.3);

the lower top surface of each swimming barrel (1.4) is flush with the lower top surface of the square inner grid frame (1.3) and is attached to the upper top surface of the square inner partition plate (1.2) with holes.

6. The device for evaluating the test for the fatigue-relieving function of the mouse according to claim 1, wherein:

each digital display timer (4.1) comprises a timing digital display screen (4.1a), and a timing start button (4.1b), a timing end button (4.1c) and a timing reset button (4.1d) which are arranged on one side of the timing digital display screen (4.1 a);

every digital display timer (4.2) all contains count down digital display screen (4.2a) and sets up count down start button A (4.2B), count down end button A (4.2c), function adjusting key A (4.2d) and the setting of count down digital display screen (4.2a) one side are in count down start button B (4.2e), count down end button B (4.2f), function setting key B (4.2g) of count down digital display screen (4.2a) opposite side.

7. The device for evaluating the test for the fatigue-relieving function of the mouse according to claim 1, wherein: four universal casters (1.10) and four reversible supporting legs (1.11) are fixedly arranged on the outer bottom wall of the square outer frame (1.1).

8. The device for evaluating the test for the fatigue-relieving function of the mouse according to claim 1, wherein: the height of each swimming barrel (1.4) is 400-500 mm and is made of organic glass;

the square outer frame (1.1) is a square frame with an upper end and an outer peripheral flanging opening, a flanging position at the rear side of the upper end of the square outer frame (1.1) and a flanging position at the front side of the upper end of the square outer frame (1.1) are equally provided with a row of labels (1.12), wherein the labels (1.12) at the flanging position at the rear side of the upper end of the square outer frame (1.1) correspond to a plurality of swimming barrels (1.4) at the rear row of the square outer frame (1.1) one by one, and the labels (1.12) at the flanging position at the front side of the upper end of the square outer frame (1.1) correspond to a plurality of swimming barrels (1.4) at the front row of the square outer frame (1.1) one by one.