CN106047743A - A microbial composition containing active floras, a preparing method thereof and applications of the composition - Google Patents

A microbial composition containing active floras, a preparing method thereof and applications of the composition Download PDF

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CN106047743A
CN106047743A CN201610270781.5A CN201610270781A CN106047743A CN 106047743 A CN106047743 A CN 106047743A CN 201610270781 A CN201610270781 A CN 201610270781A CN 106047743 A CN106047743 A CN 106047743A
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weight portions
fermentation
solid medium
weight
solid
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麦伟良
何肇铭
张茂新
郭升程
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Zhaoqing Rongzhen Biological Technology Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

A microbial composition containing active floras is provided. The composition comprises solid culture media A and B and a liquid culture medium. The solid culture medium A comprises 60-90 parts by weight of wheat bran, 10-30 parts by weight of soybean powder and 70-80 parts by weight of water. The solid culture medium B comprises 70-95 parts by weight of wheat bran, 5-20 parts by weight of rice bran and 45-50 parts by weight of water. The liquid culture medium comprises 0.5-0.7 part by weight of yeast cream, 1.0-1.2 parts by weight of peptone and 1.5-2.0 parts by weight of glucose, with the balance being water. A method for preparing the composition and applications of the composition are also provided. During a process in which the composition is used for preparing fertilizers and feed, efficient reproduction can be achieved. The composition has extremely high functions for healthy growth of crops when being used for preparing fertilizers, and has extremely high functions for healthy growth of livestock and poultry when being used for preparing probiotics added into feed. The preparing method is simple and prone to popularization and application.

Description

Containing the activity microbial composite of flora, preparation method and application
Technical field
The present invention relates to microorganism field, be especially adapted for use in micro-containing activity flora of plant husbandry and aquaculture a kind of Biological composition and preparation method.
Background technology
In the prior art, plant husbandry and aquaculture etc., because using inorganization material, bring various drawback to this field.
In plant husbandry, because life-time service chemical fertilizer, pesticide etc. cause the bad habit circulation of soil regime, the most prominent is soil Earth hardens, and permeability is poor, and institute is needed for crops can only absorb 1/3rd, the N P K of another 2/3rds and crop to fertilize Trace element is cured by the soil art problem.In terms of proportion of crop planting, existing crops can be because using by making With pesticide suppression harmful microbe breeding, reduce pest and disease damage and occur, but on the use of pesticide meeting vegetable and fruit, residual pesticide is residual Slag and the health to people cannot be cleaned up and damage.
In aquaculture field, due to threpsology and the high development of livestock products physiology, bring very to the feedstuff of animal husbandry Much progress.In order to improve Livestock Production, although mixed fodder by nutrition centered by its achievement is had been made at present and does not allows Ignore.But on the other hand, it is same as the continuous improvement of the raising of productivity ratio and the feedstuff environment of field and kind, makes domestic animal reduce Tolerance to diseases and resistivity, cause weakening of an ancient swallowtailed flag reason function, thus occur in that physiological barrier, malfunction, dysbolismus etc. Various disease conditions.Then exploitation mitigation medicine is occurred in that and to adding antibiotic in mixed fodder in a large number, preventive drug, vitamin etc. As general knowledge expected, then become new social problem, from the perspective of food public hazards, also should investigate these drawbacks, be allowed to The requirement of food hygiene law must be reached.
Summary of the invention
To this end, the technical problem to be solved is, for above-mentioned the deficiencies in the prior art, it is provided that one contains The microbial composite of activity flora, said composition can be used for preparing microbial manure, feedstuff etc., uses this fertilizer, feedstuff During can produce senior ethanol catabolic enzyme quickly and efficiently, provide well battalion for producing the lactobacillus of suppression pathogen The source of supporting, thus breed efficiently, for preparing fertilizer, the healthy growth of crops is had effect greatly, make the product of crops Amount is greatly improved, and the quality of product increases, and mouthfeel is improved, for preparing the feed additive healthy growth to poultry Also there is effect greatly.
Another technical problem that the invention solves the problems that is to provide the system of a kind of microbial composite containing activity flora Preparation Method, this preparation method is simple, and operating condition is easy to control, culture medium raw material wide material sources, cheap, with short production cycle, Production cost is low, favourable popularization and application.
For solving above-mentioned technical problem, the present invention solves the technical scheme of this technical problem and is: provide one containing active The microbial composite of flora, said composition containing bacterial population 1.0～4.0 × 108cfu/g, yeast count 3.5～8.0 × 105cfu/g, the compositions of fungi count 2.5～5.5 × 106cfu/g, its water content≤15%；Compositions includes solid medium A, solid medium B, solid medium C and fluid medium, described solid medium A, solid medium B and solid culture Base C takes phosphate solubilizing bacteria, potassium solubilizing bacteria, mycete, lactic acid bacteria, bacillus cereus, lactic acid bacteria, bacillus bifidus, bacillus subtilis respectively, carries out Strain is inoculated；Wherein, solid medium A includes the Testa Tritici of 60～90 weight portions, the analysis for soybean powder of 10～30 weight portions, 70～80 weights The water of amount part；Solid medium B includes the Testa Tritici of 70～95 weight portions, the Testa oryzae of 5～20 weight portions, 45～50 weight portions Water；Solidification culture medium C includes the Testa Tritici of 40～60 weight portions, the Testa oryzae of 35～55 weight portions, the brown sugar of 1～5 weight portions, 40～ The water of 60 weight portions；Fluid medium includes that percentage by weight is: 0.5～0.7 yeast extract；1.0～1.2 peptones；1.5～ 2.0 glucoses, remaining is water.
Further, the pH value of described liquid culture bacterium is 6～7.0.
What the present invention provided another solution is that the preparation side providing a kind of microbial composite containing activity flora Method, comprises the following steps:
(1) purebred amplification culture: solid medium A is placed in the transparent vessel of 500～2000ml, sterilizing, takes respectively Phosphate solubilizing bacteria, potassium solubilizing bacteria, mycete, lactic acid bacteria, bacillus cereus, lactic acid bacteria, bacillus bifidus, the strain of bacillus subtilis is inoculated respectively In solid medium A, it is positioned in calorstat and cultivates 48～72 hours at 25～35 DEG C；
(2) liquid fermentation and culture: take saccharomycetic strain 3-7ml and be inoculated in fluid medium, in 25～35 DEG C of constant temperature Shaken cultivation cultivates 15～25 hours to obtain fermentation liquid；
(3) solid fermentation cultivate: in fermentation tank add solid medium B, take solid medium B weight 0.06～ The fermentation liquid through step (2) liquid fermentation and culture gained of 0.2% and respectively take the 0.1～0.3% of solid medium B weight through step Suddenly the phosphate solubilizing bacteria of (1) purebred amplification culture, potassium solubilizing bacteria, mycete, lactic acid bacteria, bacillus cereus, lactic acid bacteria, bacillus bifidus, hay bud Spore bacillus is accessed in the solid medium B of fermentation tank, in temperature 25～40 DEG C, regulates fermentation tank ventilation to 0.5-0.7 cube M/min, cultivate 36 hours to obtain solid culture；
(4) solid culture of step (3) gained is dried below 50～70 DEG C, controls the water content of resulting composition ≤ 10%.
Further, described step (3) solid fermentation cultivation is active in containing obtained by the cultivation of one-level solid fermentation Second order fermentation is carried out further after the compositions of microorganism species.
Further, described second order fermentation refers to take the group of the active microorganism flora that one-level solid fermentation cultivates gained Compound is cultivated as in 1% access solid medium C of solid medium C weight, cultivation temperature 45～60 DEG C, and turning is led to Gas, is dried after cultivating 72～86 hours, water content≤10% of control composition, gained bacterium Han active microorganism below 65 DEG C The compositions of group is 1.5～2.0 × 10 containing bacterial population8Cfu/g, yeast count is 3.5～4.0 × 105Cfu/g, fungi count is 2.5～3.0 × 106cfu/g。
Further, described solidification culture medium C includes the Testa Tritici of 40～60 weight portions, the Testa oryzae of 35～55 weight portions, 1 ～5 brown sugar of weight portion, the water of 40～60 weight portions.
The present invention also provides for the purposes of a kind of microbial composite containing activity flora, exists including this microbial composite Prepare the application in microbial manure or microbiological feed.
The technique scheme of the present invention has the advantage that compared to existing technology
1, the compositions containing active microorganism flora of the present invention is used, during being used for preparing fertilizer, feedstuff Senior ethanol catabolic enzyme can be produced quickly and efficiently, provide good nutrient source for producing the lactobacillus of suppression pathogen, Thus breed efficiently, for preparing fertilizer, the healthy growth of crops being had effect greatly, the yield making crops is big Big raising, the quality of product increases, and mouthfeel is improved.For preparing feed additive, the healthy growth of poultry is also had Effect greatly
2, the product preparation method of the present invention is simple, and operating condition is easy to control, culture medium raw material wide material sources, and price is low Honest and clean, with short production cycle, production cost is low, favourable popularization and application.
3, the present invention uses microbial liquid to combine with solid fermentation technique, can preferably solve in solid fermentation process, Fermentation period is long, easily pollutes, and various fermentation conditions are difficult to control to problem, it is ensured that product quality.
Detailed description of the invention
Hereinafter incite somebody to action, use following example that the present invention is further elaborated.
The present invention provides a kind of microbial composite containing activity flora, said composition containing bacterial population 1.0～4.0 × 108cfu/g, yeast count 3.5～8.0 × 105cfu/g, the compositions of fungi count 2.5～5.5 × 106cfu/g, its water content ≤ 15%；Compositions includes solid medium A, solid medium B, solid medium C and fluid medium；Wherein, solid training Support base A and include the Testa Tritici of 60～90 weight portions, the analysis for soybean powder of 10～30 weight portions, the water of 70～80 weight portions；Solid medium B Including the Testa Tritici of 70～95 weight portions, the Testa oryzae of 5～20 weight portions, the water of 45～50 weight portions；Solidification culture medium C include 40～ The Testa Tritici of 60 weight portions, the Testa oryzae of 35～55 weight portions, the brown sugar of 1～5 weight portions, the water of 40～60 weight portions；Liquid culture Base includes that percentage by weight is: 0.5～0.7 yeast extract；1.0～1.2 peptones；1.5～2.0 glucoses, remaining is water, described The pH value of liquid culture bacterium be 6～7.0.
In the present invention, described solid medium A, solid medium B and solid medium C take phosphate solubilizing bacteria, solution respectively Potassium bacterium, mycete, lactic acid bacteria, bacillus cereus, lactic acid bacteria, bacillus bifidus, bacillus subtilis carries out strain inoculation.
The preparation method of the microbial composite containing activity flora of the present invention, comprises the following steps:
(1) purebred amplification culture: solid medium A is placed in the transparent vessel of 500～2000ml, sterilizing, takes respectively Phosphate solubilizing bacteria, potassium solubilizing bacteria, mycete, lactic acid bacteria, bacillus cereus, lactic acid bacteria, bacillus bifidus, the strain of bacillus subtilis connects respectively Plant in solid medium A, be positioned in calorstat and cultivate 48～72 hours at 25～35 DEG C；
(2) liquid fermentation and culture: take saccharomycetic strain 3-7ml and be inoculated in fluid medium, in 25～35 DEG C of constant temperature Shaken cultivation cultivates 15～25 hours to obtain fermentation liquid；
(3) solid fermentation cultivate: in fermentation tank add solid medium B, take solid medium B weight 0.06～ The fermentation liquid through step (2) liquid fermentation and culture gained of 0.2% and respectively take the 0.1～0.3% of solid medium B weight through step Suddenly the phosphate solubilizing bacteria of (1) purebred amplification culture, potassium solubilizing bacteria, mycete, lactic acid bacteria, bacillus cereus, lactic acid bacteria, bacillus bifidus, hay bud Spore bacillus is accessed in the solid medium B of fermentation tank, in temperature 25～40 DEG C, regulates fermentation tank ventilation to 0.5-0.7 cube M/min, cultivate 36 hours to obtain solid culture；Solid fermentation cultivation is active in containing obtained by the cultivation of one-level solid fermentation Second order fermentation is carried out further after the compositions of microorganism species；Described second order fermentation refers to that taking one-level solid fermentation cultivates institute The compositions of the active microorganism flora obtained is cultivated as in 1% access solid medium C of solid medium C weight, training Supporting temperature 45～60 DEG C, turning is ventilated, dry below 65 DEG C after cultivating 72～86 hours, and the water content of control composition≤ 10%, the gained compositions containing active microorganism flora is 1.5～2.0 × 10 containing bacterial population8Cfu/g, yeast count be 3.5～ 4.0×105Cfu/g, fungi count is 2.5～3.0 × 106cfu/g。
(4) solid culture of step (3) gained is dried below 50～70 DEG C, controls the water content of resulting composition ≤ 10%.
The microbial composite that the embodiment of the present invention contains activity flora is for preparing microbial manure or microorganism is raised Application in material.
Embodiment 1
The preparation of the microbial composite containing activity flora:
The preparation method of above-mentioned 1-7 group of formula is as follows:
(1) purebred amplification culture: solid medium A is placed in the transparent vessel of 500～2000ml, preferably 600ml, 800ml or 1500ml, sterilizing, take phosphate solubilizing bacteria, potassium solubilizing bacteria, mycete respectively, lactic acid bacteria, bacillus cereus, lactic acid bacteria, bacillus bifidus, The strain of bacillus subtilis is inoculated in solid medium A respectively, is positioned in calorstat and cultivates 48～72 at 25～35 DEG C Hour, preferable temperature is 30 DEG C, and the preferably time is 55 hours or 60 hours；
(2) liquid fermentation and culture: take saccharomycetic strain 3～7ml and be inoculated in fluid medium, in 25～35 DEG C of constant temperature Shaken cultivation cultivates 15～25 hours to obtain fermentation liquid；Preferably strain is 4ml or 6ml, and temperature is 30 DEG C, and preferably incubation time is 20 Hour.
(3) solid fermentation cultivate: in fermentation tank add solid medium B, take solid medium B weight 0.06～ The fermentation liquid through step (2) liquid fermentation and culture gained of 0.2% and respectively take the 0.1～0.3% of solid medium B weight through step Suddenly the phosphate solubilizing bacteria of (1) purebred amplification culture, potassium solubilizing bacteria, mycete, lactic acid bacteria, bacillus cereus, lactic acid bacteria, bacillus bifidus, hay bud Spore bacillus is accessed in the solid medium B of fermentation tank, in temperature 25～40 DEG C, regulates fermentation tank ventilation to 0.5 cube m/min Clock, cultivates 36 hours to obtain solid culture；Solid fermentation cultivate through one-level solid fermentation cultivate obtained by containing active micro-life Second order fermentation is carried out further after the compositions of thing flora；Described second order fermentation refers to that taking one-level solid fermentation cultivates gained The compositions of active microorganism flora is cultivated as in 1% access solid medium C of solid medium C weight, cultivates temperature Spending 45～60 DEG C, turning is ventilated, and is dried, water content≤10% of control composition after cultivating 72～86 hours below 65 DEG C, The gained compositions containing active microorganism flora is 1.5～2.0 × 108cfu/g containing bacterial population, yeast count is 3.5～4.0 × 105cfu/g, fungi count is 2.5～3.0 × 106cfu/g.The cultivation temperature of solid medium B preferably 30 DEG C；Solid medium C Cultivation temperature preferably 50 DEG C, incubation time preferably 80 hours.
(4) being dried below 50～70 DEG C by the solid culture of step (3) gained, preferable temperature is 60 DEG C, controls institute Obtain water content≤10% of compositions, obtain the microbial composite containing activity flora.
Embodiment 2
One, saccharomycetic source, cultivate and preserve
Source: wild yeasts bacterium obtained by isolation and selection from the soil in the Fructus Litchi orchard of plantation.
Cultivate: the culture medium of employing: by peeling potatoes, be cut into small pieces, add water boil 30～45 minutes, filter, incited somebody to action Rhizoma Solani tuber osi after filter mixes sterilizing at one atm 18～30 minutes with glucose and water according to weight ratio 10:1:50.
Strain culturing: be inoculated in above-mentioned culture medium by yeast, is placed in the incubator of 32～35 DEG C cultivation 3～5 days Obtain barms.
Bacterial strain preserves:
Slant culture based formulas: yeast extract 0.5～0.8% peptone 1.0～1.3% glucose 0.5～0.8% are white Saccharum Sinensis Roxb. 1.0～1.3% agar 2% PH=6.4 remaining be water.One atmospheric pressure, sterilizing 20 minutes.
Culture medium is made slant tube, after streak inoculation is cultivated, places 3～5 DEG C of Refrigerator stores.
Two, the preparation of effective beneficial microbe compositions
(1) purebred amplification culture: make solid medium A after weighing Testa oryzae 1000g water 700g stirring and evenly mixing, be divided in In the triangular flask of 500ml, the culture medium A of every bottled 300g, 0.1MPa sterilizing takes phosphate solubilizing bacteria, potassium solubilizing bacteria, mycete in 30 minutes respectively, Lactic acid bacteria, bacillus cereus, lactic acid bacteria, bacillus bifidus, the strain of bacillus subtilis be inoculated in the most respectively equipped with In the triangular flask of culture medium, it is placed in calorstat cultivation, cultivates 72 hours at 30～33 DEG C；
(2) liquid fermentation and culture:
After weighing yeast extract 5g, peptone 10g, grape sugar 20g, water 1000g stirring and dissolving, pH value is 6.5 to make liquid culture Base, is divided in the triangular flask of 500ml, every bottled 250ml, and 0.1MPa sterilizing 30 minutes, the barms of Example 1 is in nothing It is inoculated under the conditions of bacterium equipped with in the triangular flask of fluid medium, is placed in shaking table, within 24 hours, must send out in 30～32 DEG C of shaken cultivation Ferment liquid.
(3) solid fermentation is cultivated:
Weigh 450 kilograms of wheat bran, 50 kilograms of Testa oryzae, the water yield are 250kg, place and make solid culture in 2 hours after stirring and evenly mixing Base, after sterilizing, aseptically adds the 300g phosphate solubilizing bacteria of above-mentioned cultivation, potassium solubilizing bacteria, mycete, lactic acid bacteria, bacillus cereus, breast Acid bacterium, bacillus bifidus, the fermentation liquid of bacillus subtilis, stir, regulate fermentation tank ventilation to 0.5-0.7 cubic meters per minute, Controlling temperature to cultivate 36 hours 31～34, discharging obtains solid culture；
(4) solid culture of gained is dried below 70 DEG C, and the water content of resulting composition is 10%, by GB 4789- 94 microbiological test of food hygiene standards and NY/T 227-94 standard test obtain the bacterial population of said composition be 3.0 × 108Cfu/g, yeast count is 7.0 × 105Cfu/g, fungi count is 5.0 × 106cfu/g
Implement fall 3
Saccharomycetic source, cultivate and preserve same embodiment 2, do not repeat with this.
The preparation of microbial composite
(1) purebred amplification culture: make solid medium A after weighing Testa Tritici 2000g water 1500g stirring and evenly mixing, be divided in In the triangular flask of 500ml, the culture medium A of every bottled 250g, 0.1MPa sterilizing takes phosphate solubilizing bacteria, potassium solubilizing bacteria, mycete in 30 minutes respectively, Lactic acid bacteria, bacillus cereus, lactic acid bacteria, bacillus bifidus, the strain of bacillus subtilis be inoculated in the most respectively equipped with In the triangular flask of culture medium, it is placed in calorstat cultivation, cultivates 48 hours at 30～33 DEG C；
(2) liquid fermentation and culture:
After weighing yeast extract 80g, peptone 130g, grape sugar 150g, water 9640g stirring and dissolving, pH value is 6.5 7.0 to make liquid Body culture medium, is loaded in the fermentation tank of 5L, and 0.1MPa sterilizing 30 minutes, the barms of Example 1 aseptically connects Kind, under logical oxygen, control 30～32 DEG C under the rotating speed stirring of 150rpm/min, cultivate 18 hours to obtain fermentation liquid.
(3) solid culture of gained is dried below 65 DEG C, and the water content of resulting composition is 10%, by GB/T It is 4.0 × 10 that 4789-94 and NY/T 227-94 standard test obtains the bacterial population of said composition8Cfu/g, yeast count is 8.0 × 105Cfu/g, fungi count is 5.5 × 106cfu/g
(4) two grades of solid fermentations:
Weigh 480 kilograms of Testa oryzae, 500 kilograms of Testa Tritici, 20 kilograms of brown sugar, 550 kilograms of water are made solid medium C and are separately weighed State the compositions 10 kilograms of (4) gained
Preparation method:
1. brown sugar is dissolved in 20 kilograms of more than 90 DEG C hot water, agitated makes it all dissolve, then pour the clean of outfit into In clear water standby.
2. Testa oryzae, Testa Tritici and original seed are poured in container and fully mix.
3. brown sugar water is uniformly added in raw material and fully mixes.
4. pouring in fermentation tank by the mixture mixed and stirred, thickness of feed layer is 40 50 centimeters, is struck off on surface, covers fiber crops Bag carries out natural fermentation, controls temperature less than 60 DEG C.
Once, material is dried below 70 DEG C by fermentation for 120 hours immediately altogether in turning in the most every 24 hours. The composition measuring water content of gained is 5.8%, obtains said composition by GB/T 4789-94 and NY/T 227-94 standard test Bacterial population is 1.8 × 108Cfu/g, yeast count is 4.0 × 105Cfu/g, fungi count is 2.8 × 106cfu/g
Embodiment 4 is fermented the preparation of fertilizer
The part of the most ibid embodiment 3 is only described in detail by the embodiment of the present invention, and same section does not repeats.
A. take 1.5 kilograms of brown sugar and add after 20 kg of water are boiled cool to less than 40 DEG C, pour 30 kilograms of Testa oryzae and embodiment 3 into Effectively beneficial microbe compositions 10 kilograms, and be uniformly mixed
B, by bone meal 100 kilograms, Faeces Columba livia 200 kilograms, bean cake 100 kilograms, fish flour 100 kilograms, 30 kilograms of Testa oryzae waits to be needed Raw material and mountain soil 500 kilograms, the mixing of 450 kilograms of water
C, again a and b is uniformly mixed after pile chevron and carry out natural fermentation.
D. every turning in 24 hours once, altogether after fermentation three days less than 65 DEG C dry can make Fertilizer application.
Containing bacterial population 5.0 × 10 in prepared fertilizer7Cfu/g, yeast count 4.0 × 104Cfu/g, fungi count 2.0 × 104cfu/g
Use the above-mentioned fermentation fertilizer made, be used for planting Fructus Momordicae charantiae and other fertilizer is as follows to having a competition
The experiment date: on JIUYUE 1st, 2014
The detection date: on December 5th, 2014
Test method: the experiment planted for Fructus Momordicae charantiae with 4 kinds of different fertilizer, 4 kinds of fertilizer carry out six parallel processing altogether, Each process carries out three repetitions, carries out detection purpose mensuration after each sample plot results.The method of fertilising is identical with quantity.
Test specimen:
Sample 1: use the fermentation fertilizer of embodiment 4 present invention,
Sample 2: use planting experiment base self-control farm organic fertilizer
Sample 3: use commercially available compoiste fertilizer (composition in plerosis Hefei).
Sample 4: use compound organic and inorganic fertilizer (base manure and topdress) (composition in plerosis Hefei).
Result of the test:
Conclusion
From testing result it can be seen that the Fructus Momordicae charantiae planting experiment carried out with 4 kinds of different fertilizer, the Fructus Momordicae charantiae planted out is also Raw sugar, crude protein, Vitamin C content aspect remain basically stable, and difference is inconspicuous, but other fertilizer of product average specific of sample 1 exceeds 13.8%.
The preparation of embodiment 5 feed additive
The part of the most ibid embodiment 3 is only described in detail by the embodiment of the present invention, and same section does not repeats.
1, material: effective beneficial microbe compositions 10 kilograms of 3 kilograms of embodiments 3 of brown sugar, conch meal 20 kilograms, bone meal 40 kilograms, 25 kilograms of Testa oryzae, 1000 kilograms of 40 kilograms of clean mountains of fish flour soil
2, brown sugar adds water 450 kilograms and boils latter cool to less than 40 DEG C, is poured on the effective beneficial microbe of Testa oryzae of mix homogeneously In compositions, and stir again with conch meal 20 kilograms, bone meal 40 kilograms, 25 kilograms of Testa oryzae, 40 kilograms of clean mountains of fish flour soil 1000 kilograms of mixings.
3, the material that top mixes being placed in fermentation tank to pile up, the thickness of the bed of material is every 24 hours after 40～50 centimeters Once, fermentation can be dried for five days at 65 DEG C altogether in turning
Containing bacterial population 5.0 × 10 in the feed probiotic that this fermentation completes7Cfu/g, yeast count 4.0 × 104Cfu/g, mould Bacterium number 2.0 × 104cfu/g
Test method: piglets 30, is randomly divided into three groups, often group 10, wherein boar 7, sow 3.The nursing time, Mode, condition are identical
Test group 1: the embodiment of the present invention 6 fermented feed probiotic bacteria 5%, premix material (a kind of mixed fodder additive) 2%, Any mixture such as other 94% Semen Maydis powder, wheat bran, Testa oryzae, bean cake make forage feed
Test group 2: the embodiment of the present invention 6 fermented feed probiotic bacteria 5%, other 96% Semen Maydis powder, wheat bran, Testa oryzae, bean cake Forage feed is made etc. any mixture
Matched group 3: make feedstuff by any mixture such as premix material 4%, other 96% Semen Maydis powder, wheat bran, Testa oryzae, bean cake and feed Foster result of the test:
Conclusion: as pig feed probiotic bacteria 5%, feed piglets, effectively prevent and treat the red Hakuri of piglet, reduce diarrhea rate 40%) more than, improving piglet grain conversion ratio 17%, daily gain improves 20%, improves more than 14% than matched group.Reach country Secondary discharge standard.
Obviously, above-described embodiment is only for clearly demonstrating example, and not restriction to embodiment.Right For those of ordinary skill in the field, can also make on the basis of the above description other multi-form change or Variation.Here without also cannot all of embodiment be given exhaustive.And the obvious change thus extended out or Change among still in the protection domain of the invention.

Claims (8)

1. the microbial composite containing activity flora, it is characterised in that: said composition containing bacterial population 1.0～4.0 × 108cfu/g, yeast count 3.5～8.0 × 105cfu/g, the compositions of fungi count 2.5～5.5 × 106cfu/g, its water content ≤ 15%；Compositions includes solid medium A, solid medium B, solid medium C and fluid medium, described solid training Support base A, solid medium B and solid medium C and take phosphate solubilizing bacteria, potassium solubilizing bacteria, yeast, mycete, lactic acid bacteria, spore bar respectively Bacterium, lactic acid bacteria, bacillus bifidus, bacillus subtilis, carry out strain inoculation；
Wherein,
Solid medium A includes the Testa Tritici of 60～90 weight portions, the analysis for soybean powder of 10～30 weight portions, the water of 70～80 weight portions；
Solid medium B includes the Testa Tritici of 70～95 weight portions, the Testa oryzae of 5～20 weight portions, the water of 45～50 weight portions；
Solidification culture medium C includes the Testa Tritici of 40～60 weight portions, the Testa oryzae of 35～55 weight portions, the brown sugar of 1～5 weight portions, 40 ～60 water of weight portion；
Fluid medium includes that percentage by weight is: 0.5～0.7 yeast extract；1.0～1.2 peptones；1.5～2.0 glucoses, Remaining is water.
Microbial composite containing activity flora the most according to claim 1, it is characterised in that: described liquid culture The pH value of base is 6～7.0.
3. the preparation method of the microbial composite containing activity flora, it is characterised in that: comprise the following steps:
(1) purebred amplification culture: solid medium A is placed in the transparent vessel of 500～2000ml, sterilizing, takes phosphorus decomposing respectively Bacterium, potassium solubilizing bacteria, yeast, mycete, lactic acid bacteria, bacillus cereus, lactic acid bacteria, bacillus bifidus, bacillus subtilis, strain respectively It is inoculated in solid medium A, is positioned in calorstat and cultivates 48～72 hours at 25～35 DEG C；
(2) liquid fermentation and culture: take saccharomycetic strain 3-7ml and be inoculated in fluid medium, in 25～35 DEG C of constant temperature oscillations Cultivate 15～25 hours to obtain fermentation liquid；
(3) solid fermentation is cultivated: adds solid medium B in fermentation tank, takes the 0.06～0.2% of solid medium B weight The fermentation liquid through step (2) liquid fermentation and culture gained and respectively take the 0.1～0.3% of solid medium B weight through step (1) The phosphate solubilizing bacteria of purebred amplification culture, potassium solubilizing bacteria, mycete, lactic acid bacteria, bacillus cereus, lactic acid bacteria, bacillus bifidus, bacillus subtilis Bacterium, accesses in the solid medium B of fermentation tank, in temperature 25～40 DEG C, regulation fermentation tank ventilation to 0.5～0.7 cubic metre/ Minute, cultivate 36 hours to obtain solid culture；
(4) solid culture of step (3) gained is dried below 50～70 DEG C, the water content of control resulting composition≤ 10%.
The preparation method of the microbial composite containing activity flora the most according to claim 3, it is characterised in that: described Step (3) solid fermentation cultivate through one-level solid fermentation cultivate obtained by the compositions containing active microorganism flora laggard One step carries out second order fermentation.
The preparation method of the microbial composite containing activity flora the most according to claim 4, it is characterised in that: described Second order fermentation refer to take one-level solid fermentation and cultivate the compositions of active microorganism flora of gained by solid medium C weight 1% access solid medium C in cultivate, cultivation temperature 45～60 DEG C, turning ventilate, 65 after cultivating 72～86 hours Being dried below DEG C, water content≤10% of control composition, the final gained compositions containing active microorganism flora is containing bacterial population It is 1.5～2.0 × 108Cfu/g, yeast count is 3.5～4.0 × 105Cfu/g, fungi count is 2.5～3.0 × 106cfu/g。
6., according to the preparation method of the arbitrary described microbial composite containing activity flora of claim 3～5, its feature exists In: described solid medium A includes the Testa Tritici of 60～90 weight portions, the analysis for soybean powder of 10～30 weight portions, 70～80 weight portions Water；Solid medium B includes the Testa Tritici of 70～95 weight portions, the Testa oryzae of 5～20 weight portions, the water of 45～50 weight portions；Liquid Body culture medium includes that percentage by weight is: 0.5～0.7 yeast extract；1.0～1.2 peptones；1.5～2.0 glucoses, remaining is Water.
The preparation method of the microbial composite containing activity flora the most according to claim 6, it is characterised in that: described Solidification culture medium C include the Testa Tritici of 40～60 weight portions, the Testa oryzae of 35～55 weight portions, the brown sugar of 1～5 weight portions, 40～ The water of 60 weight portions.
8. the purposes of the microbial composite containing activity flora, it is characterised in that: include that this microbial composite is in system Application in standby microbial manure or microbiological feed.
CN201610270781.5A 2016-04-27 2016-04-27 A microbial composition containing active floras, a preparing method thereof and applications of the composition Pending CN106047743A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112322562A (en) * 2020-12-25 2021-02-05 广西壮族自治区畜牧研究所 Solid culture medium of bacillus, preparation method thereof and bacillus culture method

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Publication number Priority date Publication date Assignee Title
CN1934989A (en) * 2006-09-04 2007-03-28 利耀铭 Microbial fodder additive
CN1955276A (en) * 2006-09-04 2007-05-02 利耀铭 Organic microbial composite and use

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1934989A (en) * 2006-09-04 2007-03-28 利耀铭 Microbial fodder additive
CN1955276A (en) * 2006-09-04 2007-05-02 利耀铭 Organic microbial composite and use

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112322562A (en) * 2020-12-25 2021-02-05 广西壮族自治区畜牧研究所 Solid culture medium of bacillus, preparation method thereof and bacillus culture method

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