CN106047704B - A kind of production line of pilot scale culture plant stem cell - Google Patents
A kind of production line of pilot scale culture plant stem cell Download PDFInfo
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- CN106047704B CN106047704B CN201610381074.3A CN201610381074A CN106047704B CN 106047704 B CN106047704 B CN 106047704B CN 201610381074 A CN201610381074 A CN 201610381074A CN 106047704 B CN106047704 B CN 106047704B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/08—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by vibration
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M43/00—Combinations of bioreactors or fermenters with other apparatus
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Abstract
The invention discloses a kind of production line of pilot scale culture plant stem cell, including the filling freezing inoculation line of pallet cleaning, drying line and culture medium；The pallet cleaning, drying line includes the first conveyer belt, cleaning storehouse, blows sump and dryness storehouse, and first conveyer belt sequentially passes through the cleaning storehouse, described blows sump and the dryness storehouse；The filling freezing inoculation line of culture medium includes the second conveyer belt, automatic filling device, cool chamber and inoculation device, the automatic filling device, the cool chamber and the inoculation device are sequentially arranged on the direction of transfer of second conveyer belt, high efficiency particulate air filter, wind turbine and condenser are equipped with inside the cool chamber successively, the inoculation device is equipped with hopper, and the hopper is arranged on above second conveyer belt.The invention enables the thinkings that conventional liquid suspension culture is avoided on the industrialized agribusiness of plant stem cell, have developed simpler, more efficient solid culture technical system.
Description
Technical field
Technical field more particularly to a kind of life of pilot scale culture plant stem cell the present invention relates to cell culture system
Producing line.
Background technology
It is typically efficiently with practicality that fermentor liquid suspension, which is cultivated for the factorial praluction of microorganism, this is big
The production example of amount is confirmed.The big decades of times of volume ratio microorganism of plant cell, to the various stress in suspension incubation
Compare sensitive；Liquid suspension culture volume often amplifies level-one, it is necessary to study new condition of culture, the final culture body that can amplify
Product is a unknown number, this causes the process of pilot scale can very long, costly and result uncertainty.This point is many in the past
It is confirmed in the suspension culture of plant cell.Even if the cost of investment of fermentation tank is bought in pilot scale success in large-scale production
Electric power energy and generate substantial amounts of waste water very high, that production process is more complicated, consumption is more.The proliferating cycle of different plant cells
Up to 3-10 days, a complete fermented and cultured cycle was typically for up to 2-4 months, this causes to can be only done several production weeks every year
Phase, ultimate output are restricted.So the suspension culture of only a small number of plant cells obtains in production practices over the past several decades
It must succeed.
Solid culture technology is not required one progressively to amplify the pilot scale of volume of culture culture as fermentor liquid suspends
Process, after solid culture Establishing, it is possible to be factory produced, because one basin cell of culture is with cultivating tens thousand of basins
Technical method used in cell is the same, so highly shortened processes of research ＆ development and simplify production technology.Due to plastics
Culture basin is dirt cheap, and the cost of investment in large-scale production is relatively low.Cell inoculation is being put to a needs after culture medium
Dark culturing can be proliferated 10 times in 30 days in putting between culture of the temperature stabilization in room temperature, so cell cultivation process is very simple,
Operation is easy, and production process is more energy efficient.Cell can be harvested after culture, solid medium is then substantially depleted, will not
Substantial amounts of waste water is generated as liquid suspension culture, so more environmentally-friendly.As long as the area of factory allows, can be daily
Thousand basin cell of inoculation number, while thousands of basin cells are harvested daily, accomplish Xun Huan production, daily production capacity can reach several tons, very
To higher.
In order to which solid culture technical system pilot scale culture plant stem cell is made to become more efficient, is easier, the present inventor
A kind of production line of pilot scale culture plant stem cell is studied and devises, thus this case generates.
The content of the invention
It is an object of the invention to provide a kind of production lines of pilot scale culture plant stem cell so that in plant stem cell
Industrialized agribusiness on avoid the thinking of conventional liquid suspension culture, to develop simpler, more efficient solid culture
Technical system.
To achieve these goals, the technical scheme adopted by the invention to solve the technical problem is that：
A kind of production line of pilot scale culture plant stem cell connects including the filling freezing of pallet cleaning, drying line and culture medium
Kind line；The pallet cleaning, drying line includes the first conveyer belt, cleaning storehouse, blows sump and dryness storehouse, first conveyer belt according to
It is secondary through the cleaning storehouse, it is described blow sump and the dryness storehouse, the cleaning storehouse is equipped with several row's nozzles, the nozzle angle
Adjustable, described blow is equipped with several air knives in sump, and the dryness storehouse blows sump closed butt joint with described, inside the dryness storehouse
Equipped with several heat-generating pipes；The filling freezing inoculation line of culture medium includes the second conveyer belt, automatic filling device, cool chamber and connects
Kind machine, the automatic filling device, the cool chamber and the inoculation device are sequentially arranged in the direction of transfer of second conveyer belt
On, the automatic filling device includes bottle placer, agitator tank and ultra high temperature short time sterilization machine, and the bottle placer is arranged on the freezing
The entrance in storehouse, the ultra high temperature short time sterilization machine be arranged between the bottle placer and the agitator tank, inside the cool chamber according to
Secondary to be equipped with high efficiency particulate air filter, wind turbine and condenser, the cool chamber inside further respectively has water receiving tank, the inoculation on two side
Machine is equipped with hopper, and the hopper is arranged on above second conveyer belt.
As the preferred embodiment of embodiment, the cleaning storehouse is equipped with 3 row's nozzles, often arranges the spray angle difference of nozzle, institute
It states cleaning storehouse and further includes movable water tank, the movable water tank is arranged on the one side of the cleaning storehouse.
As the preferred embodiment of embodiment, described blow in sump is respectively provided with 4 air knives up and down.
As the preferred embodiment of embodiment, the dryness storehouse internal temperature control is at 80-150 DEG C.
As the preferred embodiment of embodiment, the input end of the dryness storehouse and the width of the port of export are slightly larger than the width of pallet
Degree, further includes the door curtain made of cloth, two door curtains made of cloth are respectively arranged on the input end of the dryness storehouse and the port of export of the dryness storehouse.
As the preferred embodiment of embodiment, the pallet cleaning, drying line further includes the first manually sale at reduced prices area and the first closing quotation
Workbench, the described first artificial sale at reduced prices area are arranged on the input end of the cleaning storehouse, and the first closing quotation workbench is arranged on described do
The port of export in dry storehouse.
As the preferred embodiment of embodiment, the bottle placer is equipped with 4 peristaltic pumps, and the peristaltic pump is arranged on the freezing
The input end in storehouse, the bottle placer capacity are 50-500 milliliters, and the bottle placer is equipped with PLC and coordinates touch screen human-computer interface.
As the preferred embodiment of embodiment, two cool chambers are arranged in series, and are equipped at the top of the cool chamber and are sent ozone hole
And suction opening, the input end of the cool chamber and the width of the port of export further include baffle, two gears slightly larger than the width of pallet
Plate is respectively arranged in a manner that height is adjustable on the input end of the cool chamber and the port of export of the cool chamber.
As the preferred embodiment of embodiment, further include refrigerator, the refrigerator is arranged on outside the cool chamber, and with it is described
Condenser connects.
As the preferred embodiment of embodiment, the filling freezing inoculation line of culture medium further includes the second artificial sale at reduced prices Qu Ji
Two closing quotation workbench, the described second artificial sale at reduced prices area are arranged on the input end of the cool chamber, and the second closing quotation workbench is arranged on
The port of export of the inoculation device.
Since a kind of production line of pilot scale culture plant stem cell of the present invention employs above-mentioned technical solution, that is, include
Pallet cleaning, drying line and the filling freezing inoculation line of culture medium；The pallet cleaning, drying line include the first conveyer belt, cleaning storehouse,
Blow sump and dryness storehouse, first conveyer belt sequentially pass through the cleaning storehouse, it is described blow sump and the dryness storehouse, it is described clear
It brings down stocks equipped with several row's nozzles, the nozzle angle is adjusted, and described blow is equipped with several air knives, the dryness storehouse and institute in sump
It states and blows sump closed butt joint, the dryness storehouse is internally provided with several heat-generating pipes；The filling freezing inoculation line of the culture medium includes the
Two conveyer belts, automatic filling device, cool chamber and inoculation device, the automatic filling device, the cool chamber and the inoculation device
It is sequentially arranged on the direction of transfer of second conveyer belt, the automatic filling device includes bottle placer, agitator tank and superhigh temperature
Instantaneous sterilizing machine, the bottle placer are arranged on the entrance of the cool chamber, and the ultra high temperature short time sterilization machine is arranged on the bottle placer
And between the agitator tank, high efficiency particulate air filter, wind turbine and condenser are equipped with inside the cool chamber successively, inside the cool chamber
Water receiving tank is further respectively had on two side, the inoculation device is equipped with hopper, and the hopper is arranged on above second conveyer belt；Make
The invention has the advantages that：
The present invention avoids the thinking of conventional liquid suspension culture on the industrialized agribusiness of plant stem cell, develops
The solid culture technical system that is more simple and efficient.Production line of the present invention, using * 3 centimetres of * 60 centimetres of 90 centimetres (length) (width)
The pallet of (height) is inoculated with 0.1 kilogram of cell, by the quiescent culture of one month, it is possible to produce 1 kilogram of fresh cell.This hair
It is bright once to cultivate tens thousand of basin cells simultaneously, the production needs of scale are greatly facilitated, are opened so the cultivating system is one kind
Put the cultivating system of formula.
Description of the drawings
Fig. 1 is the pallet cleaning, drying cable architecture schematic diagram of the present invention；
Fig. 2 is the structure diagram of the filling freezing inoculation line of the culture medium of the present invention；
Fig. 3 is the internal structure schematic diagram of the cool chamber of the present invention；
Fig. 4 is the structure diagram of the inoculation device of the present invention.
Specific embodiment
Below in conjunction with the drawings and specific embodiments with the invention will be further described.
As shown in Figure 1 to Figure 3, a kind of production line of pilot scale culture plant stem cell, including 1 He of pallet cleaning, drying line
The filling freezing inoculation line 2 of culture medium；The pallet cleaning, drying line 1 include the first conveyer belt 11, cleaning storehouse 12, blow sump 13 and
Dryness storehouse 14, first conveyer belt 11 sequentially pass through the cleaning storehouse 12, it is described blow sump 13 and the dryness storehouse 14, it is described
Cleaning storehouse 12 is equipped with several row's nozzles, and the nozzle angle is adjusted, and described blow is equipped with several air knives, the dryness storehouse in sump
14 blow 13 closed butt joint of sump with described, and the dryness storehouse 14 is internally provided with several heat-generating pipes 141；The filling freezing of culture medium
Be inoculated with line 2 include the second conveyer belt 21, automatic filling device 22, cool chamber 23 and inoculation device 24, the automatic filling device 22,
The cool chamber 23 and the inoculation device 24 are sequentially arranged on the direction of transfer of second conveyer belt 21, the auto-filling dress
Putting 22 includes bottle placer 221, agitator tank 222 and ultra high temperature short time sterilization machine 223, and the bottle placer 221 is arranged on the cool chamber
23 entrance, the ultra high temperature short time sterilization machine 223 are arranged between the bottle placer 221 and the agitator tank 222, the freezing
High efficiency particulate air filter 231, wind turbine 232 and condenser 233 are equipped with inside storehouse 23 successively, is also divided on the 23 inside two side of cool chamber
Not She You water receiving tank 234, the inoculation device 24 is equipped with hopper 241, and the hopper 241 is arranged on second conveyer belt, 21 top.
As the preferred embodiment of embodiment, the cleaning storehouse is equipped with 3 row's nozzles, often arranges the spray angle difference of nozzle, institute
It states cleaning storehouse and further includes movable water tank 121, the movable water tank is arranged on the one side of the cleaning storehouse.
As the preferred embodiment of embodiment, described blow in sump is respectively provided with 4 air knives up and down.
As the preferred embodiment of embodiment, 14 internal temperature control of dryness storehouse is at 80-150 DEG C.
As the preferred embodiment of embodiment, the input end of the dryness storehouse and the width of the port of export are slightly larger than the width of pallet
Degree, further includes the door curtain made of cloth, two door curtains made of cloth are respectively arranged on the input end of the dryness storehouse and the port of export of the dryness storehouse.
As the preferred embodiment of embodiment, the pallet cleaning, drying line 1 further includes the first artificial sale at reduced prices area 15 and first
Closing quotation workbench 16, the described first artificial sale at reduced prices area 15 are arranged on the input end of the cleaning storehouse 12, the first closing quotation workbench
16 are arranged on the port of export of the dryness storehouse 14.
As the preferred embodiment of embodiment, the bottle placer 221 is equipped with 4 peristaltic pumps 2211, and the peristaltic pump is arranged on
The input end of the cool chamber 23,221 capacity of bottle placer are 50-500 milliliters, and the bottle placer 221 is equipped with PLC cooperations and touches
Touch screen man-machine interface.
As the preferred embodiment of embodiment, two cool chambers 23 are arranged in series, and 23 top of cool chamber is smelly equipped with sending
Oxygen hole 236 and suction opening 235, the input end of the cool chamber and the width of the port of export are slightly larger than the width of pallet, further include gear
Plate, two baffles are respectively arranged on the port of export of the input end and the cool chamber of the cool chamber in a manner that height is adjustable
On.
As the preferred embodiment of embodiment, refrigerator 25 is further included, the refrigerator 25 is arranged on outside the cool chamber 23, and
It is connected with the condenser 233.
As the preferred embodiment of embodiment, the filling freezing inoculation line 2 of culture medium further includes the second artificial sale at reduced prices area 26
And second closing quotation workbench 27, described second manually sale at reduced prices area 26 be arranged on the cool chamber 23 input end, it is described second closing quotation
Workbench 27 is arranged on the port of export of the inoculation device 24.
The pallet used in this production line is the vinyl disc of resistance to 160-170 DEG C of high temperature, class after the culture medium heating filled in disk
Aqua is similar to, g., jelly-like is similar under room temperature.The door of cleaning storehouse, dryness storehouse and cool chamber involved by this production line can be with
It is opened again in the period closing that do not work, work, door is closed so when not working to keep a relatively clean state.
Whole production line is all to be moved along disk by conveyer belt, and each section of conveyer belt installs an emergency stop switch, mounted on comparing
Obvious place operates when facilitating emergency.
The flow of this pallet cleaning, drying line is as follows：
1st, manually sale at reduced prices：First, manually sale at reduced prices area, artificial left-hand thread have struck off the pallet of culture medium, have not stacked.
2nd, clean：In cleaning storehouse, 3 row's nozzles are set, the angle for often arranging nozzle is different：One row's nozzle is vertical, row's nozzle
Always basin direction tilts, and row's nozzle is to going to basin direction to tilt, and to ensure to clean comprehensively, nozzle angle is adjustable.
3rd, air-dry：It is blown in hot wind in sump, respectively there is 4 air knives up and down, to blow the droplet of bulky grain off.
4th, dry：In dryness storehouse, dryness storehouse and sump closed butt joint is blown, the wind in dryness storehouse is cycled in inside, and temperature can
To reach 80-150 DEG C, the inlet and outlet that disk is left at both ends for just allow disk by and with the door curtain made of cloth.
5th, close：In the first closing quotation workbench, closing quotation platform band gradient；When disk is stacked to certain altitude, manually take away,
It takes away and sterilizes between sterilizing.
The flow of the filling freezing inoculation line of this culture medium is as follows：
1st, manually sale at reduced prices：Second, manually sale at reduced prices area, front are placed the pallet after sterilizing, are not stacked.
2nd, auto-filling：Bottle placer of the automatic filling device equipped with 4 peristaltic pumps, filling capacity 50-500 milliliters, program
It controls and coordinates touch screen human-computer interface for PLC；It is additionally equipped with 2 1.5 tons of heating and stirring tanks, 11 ton of agitator tank and 2 superelevation
Warm instantaneous sterilizing machine is respectively used to the configuration, storage and sterilizing of culture medium.
3rd, freeze：Equipped with 2 cool chambers, the side door of cool chamber can be adjusted arbitrarily, and the inlet and outlet that disk is left at both ends for just permit
Perhaps disk is by the way that it is adjustable that both ends doorway installs baffle height additional；2 holes of cool chamber intermediate device, one be a diameter of 15cm exhausting
Mouthful, for taking ozone away, one be sent into a diameter of 5cm that ozone is sterilized send ozone hole；It is cold in order to reduce condensed water
Freeze storehouse using plastic plate, condenser uses lower section side；Cool chamber both sides tilt installation, afterbody lower section equipped with cold water reservoir is connect
Connect hose draining, wind speed≤0.4m/s.Two concatenation refrigerators are configured with, outside cool chamber.
4th, it is inoculated with：If there are one two-way inoculation device, as shown in figure 4, the inoculation device 24 include hopper 241, stent 242,
Pneumatic vibrator 243 and air blowing device (not shown), two the second conveyer belts 21 being mutually juxtaposed are arranged on 242 lower section of stent, described
242 one side of stent is equipped with height regulating rod 244, and two hoppers 241 are slidably arranged on stent 241 respectively, and two is pneumatic
Vibrator 243 is respectively arranged on two hoppers 241, facilitates the smooth whereabouts of seed, and two air blowing devices (are done using stainless steel square tube fluting
Air knife) it is respectively arranged on one end of stent 242；The seed connect using this inoculation device is soft, size 1cm-5cm；Dress above conveyer belt
There is fender plate 245, it is uniform convenient for material.
5th, close：In the second closing quotation workbench, the estrade equipped with no-power drum convenient for being packaged, turns after plate is packed
It is cultivated between culture.
Our company has carried out a series of research and development, by adding in common bacteriostatic agent success in food industry in the medium
Ground establishes a set of efficient open culturing system, and pollution problem will not occur under the conditions of 100,000 grades of cleanliness factor.It is newly-built
The cleanliness factor of factory is set in ten thousand grades, locally reaches hundred grades so that the system successfully operates.The production line of the present invention to advise
Modelling culture plant cell becomes more efficient, is easier, and cost is lower.The production line of the present invention is exactly exclusively for solid culture skill
Art is realized plant layout and is set up.
All deformations that those of ordinary skill in the art directly can export or associate from the disclosure of invention, should all
It is considered protection scope of the present invention.
Claims (8)
1. a kind of production line of pilot scale culture plant stem cell, it is characterised in that：Including pallet cleaning, drying line and culture medium
Filling freezing inoculation line；The pallet cleaning, drying line includes the first conveyer belt, cleaning storehouse, blows sump and dryness storehouse, and described the
One conveyer belt sequentially pass through the cleaning storehouse, it is described blow sump and the dryness storehouse, the cleaning storehouse is equipped with several row's nozzles, institute
It states nozzle angle to be adjusted, described blow is equipped with several air knives in sump, and the dryness storehouse blows sump closed butt joint with described, described
Dryness storehouse is internally provided with several heat-generating pipes；The filling freezing inoculation line of the culture medium include the second conveyer belt, automatic filling device,
Cool chamber and inoculation device, the automatic filling device, the cool chamber and the inoculation device are sequentially arranged in second conveyer belt
Direction of transfer on, the automatic filling device includes bottle placer, agitator tank and ultra high temperature short time sterilization machine, and the bottle placer sets
In the entrance of the cool chamber, the ultra high temperature short time sterilization machine is arranged between the bottle placer and the agitator tank, described cold
Freeze and be equipped with high efficiency particulate air filter, wind turbine and condenser inside storehouse successively, water receiving is further respectively had on two side inside the cool chamber
Slot, the inoculation device are equipped with hopper, and the hopper is arranged on above second conveyer belt；The pallet cleaning, drying line further includes
First artificial sale at reduced prices area and the first closing quotation workbench, the described first artificial input end of the area arranged on the cleaning storehouse of selling at reduced prices are described
First closing quotation workbench is arranged on the port of export of the dryness storehouse；The filling freezing inoculation line of culture medium further includes second and manually puts
Panel and the second closing quotation workbench, the described second artificial sale at reduced prices area are arranged on the input end of the cool chamber, the second closing quotation work
Make the port of export that platform is arranged on the inoculation device.
2. a kind of production line of pilot scale culture plant stem cell as described in claim 1, it is characterised in that：The cleaning storehouse
Equipped with 3 row's nozzles, often the spray angle of row's nozzle is different, and the cleaning storehouse further includes movable water tank, the movable water tank
Arranged on the one side of the cleaning storehouse.
3. a kind of production line of pilot scale culture plant stem cell as described in claim 1, it is characterised in that：It is described to blow sump
It is interior to be respectively provided with 4 air knives up and down.
4. a kind of production line of pilot scale culture plant stem cell as described in claim 1, it is characterised in that：The dryness storehouse
Internal temperature control is at 80-150 DEG C.
5. a kind of production line of pilot scale culture plant stem cell as described in claim 1, it is characterised in that：The dryness storehouse
Input end and the width of the port of export be slightly larger than the width of pallet, further include the door curtain made of cloth, two door curtains made of cloth are respectively arranged on described dry
On the port of export of the input end in dry storehouse and the dryness storehouse.
6. a kind of production line of pilot scale culture plant stem cell as described in claim 1, it is characterised in that：The bottle placer
4 peristaltic pumps are equipped with, the peristaltic pump is arranged on the input end of the cool chamber, and the bottle placer capacity is 50-500 milliliters,
The bottle placer is equipped with PLC and coordinates touch screen human-computer interface.
7. a kind of production line of pilot scale culture plant stem cell as described in claim 1, it is characterised in that：Two described cold
Freeze storehouse to be arranged in series, be equipped at the top of the cool chamber and send ozone hole and suction opening, the input end of the cool chamber and the port of export
Width is slightly larger than the width of pallet, further includes baffle, and two baffles are respectively arranged on the freezing in a manner that height is adjustable
On the port of export of the input end in storehouse and the cool chamber.
8. a kind of production line of pilot scale culture plant stem cell as described in claim 1, it is characterised in that：Further include freezing
Machine, the refrigerator is arranged on outside the cool chamber, and is connected with the condenser.
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| CN201610381074.3A CN106047704B (en) | 2016-06-01 | 2016-06-01 | A kind of production line of pilot scale culture plant stem cell |
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| CN201610381074.3A CN106047704B (en) | 2016-06-01 | 2016-06-01 | A kind of production line of pilot scale culture plant stem cell |
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| CN106047704A CN106047704A (en) | 2016-10-26 |
| CN106047704B true CN106047704B (en) | 2018-05-29 |
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| CN108504566B (en) * | 2017-02-27 | 2023-12-12 | 深圳市赢联丰生物科技有限公司 | Standardized mass production line for non-variant homologous plant stem cells |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102174395A (en) * | 2011-01-30 | 2011-09-07 | 中国科学院广州生物医药与健康研究院 | Automation augmentation and culture system of induced pluripotent stem cells |
| CN102250767A (en) * | 2011-06-28 | 2011-11-23 | 江苏省北科生物科技有限公司 | Digitalized automatic production system for mesenchymal stem cells of umbilical cord |
| CN104207308A (en) * | 2014-07-30 | 2014-12-17 | 刘会平 | Method and system for removing seed coats in walnuts through alkali-free method pneumatic blow injection |
| CN104534845A (en) * | 2014-12-19 | 2015-04-22 | 山东圣琪生物有限公司 | Continuous vacuum belt type drying device |
| CN105349422A (en) * | 2015-12-03 | 2016-02-24 | 遵义医学院 | Plant cell suspension culture device |
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2016
- 2016-06-01 CN CN201610381074.3A patent/CN106047704B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102174395A (en) * | 2011-01-30 | 2011-09-07 | 中国科学院广州生物医药与健康研究院 | Automation augmentation and culture system of induced pluripotent stem cells |
| CN102250767A (en) * | 2011-06-28 | 2011-11-23 | 江苏省北科生物科技有限公司 | Digitalized automatic production system for mesenchymal stem cells of umbilical cord |
| CN104207308A (en) * | 2014-07-30 | 2014-12-17 | 刘会平 | Method and system for removing seed coats in walnuts through alkali-free method pneumatic blow injection |
| CN104534845A (en) * | 2014-12-19 | 2015-04-22 | 山东圣琪生物有限公司 | Continuous vacuum belt type drying device |
| CN105349422A (en) * | 2015-12-03 | 2016-02-24 | 遵义医学院 | Plant cell suspension culture device |
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