CN106047704A - Production line for culturing plant stem cells on large scale - Google Patents
Production line for culturing plant stem cells on large scale Download PDFInfo
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- CN106047704A CN106047704A CN201610381074.3A CN201610381074A CN106047704A CN 106047704 A CN106047704 A CN 106047704A CN 201610381074 A CN201610381074 A CN 201610381074A CN 106047704 A CN106047704 A CN 106047704A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/08—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by vibration
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M43/00—Combinations of bioreactors or fermenters with other apparatus
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Abstract
The invention discloses a production line for culturing plant stem cells on a large scale. The production line comprises a tray washing and drying line and a medium filling, freezing and inoculating line; the tray washing and drying line comprises a first conveying belt, a washing bin, a water blowing bin and a drying bin, and the first conveying belt sequentially penetrates through the washing bin, the water blowing bin and the drying bin; the medium filling, freezing and inoculating line comprises a second conveying belt, an automatic filling device, a freezing bin and an inoculating machine, the automatic filling device, the freezing bin and the inoculating machine are sequentially arranged in the conveying direction of the second conveying belt, an efficient filter, a draught fan and a condenser are sequentially arranged in the freezing bin, the inoculating machine is provided with a hopper, and the hopper is arranged above the second conveying belt. According to the production line, the thought of conventional liquid suspension culture is avoided during factory-like culture of the plant stem cells, and a simpler and more efficient solid culture technical system is developed.
Description
Technical field
The present invention relates to the technical field of cell culture system, particularly relate to the life of a kind of pilot scale culture plant stem cell
Produce line.
Background technology
Fermentor liquid suspension culture is typically efficient and practical for the factorial praluction of microorganism, and this is by greatly
The production example of amount is confirmed.Various during suspension culture are coerced by the big decades of times of volume ratio microorganism of plant cell
The most sensitive；Fluid suspension culture volume often amplifies one-level, it is necessary to study new condition of culture, the cultivation body that finally can amplify
Long-pending is a unknown number, this cause pilot scale process can the longest, costly with the uncertainty of result.This point is a lot of in the past
The suspension culture of plant cell is confirmed.Even if pilot scale success, buy the cost of investment of fermentation tank when large-scale production
The highest, production process is more complicated, consume more electric power energy and produce substantial amounts of waste water.The proliferating cycle of different plant cells
Up to 3-10 days, a complete fermentation culture cycle was typically for up to 2-4 month, and this causes can be only done several production week every year
Phase, ultimate output is restricted.So only having the suspension culture of minority plant cell to obtain in the production practices of past many decades
Obtain success.
Solid culture technology need not the pilot scale of a progressively amplification culture volume as fermentor liquid suspension culture
Process, once after solid culture Establishing, it is possible to carry out factorial praluction, because cultivating a basin cell and cultivating tens thousand of basins
Technical method used by cell is the same, so highly shortened processes of research & development and simplifying production technology.Due to plastics
Culture basin is dirt cheap, and the cost of investment when large-scale production is relatively low.Cell has only to after being inoculated into culture medium putting
Put dark culturing in temperature stabilization is between the cultivation of room temperature and can breed 10 times in 30 days, so cell cultivation process is very simple,
Processing ease, production process is more energy efficient.Cultivation gets final product harvesting after terminating, solid medium is the most depleted, will not
Substantial amounts of waste water is produced as fluid suspension culture, thus more environmentally-friendly.As long as the area of factory allows, can every day
Inoculating thousands of basin cell, gather in the crops thousands of basin cells every day simultaneously, accomplish cyclic production, production capacity just can reach several tons, very every day
To higher.
In order to make solid culture technical system pilot scale culture plant stem cell become more efficient, be easier to, the present inventor
Studying and devise the production line of a kind of pilot scale culture plant stem cell, this case thus produces.
Summary of the invention
It is an object of the invention to provide the production line of a kind of pilot scale culture plant stem cell so that at plant stem cell
Industrialized agribusiness on avoid the thinking of fluid suspension culture of routine, to develop simpler, more efficient solid culture
Technical system.
To achieve these goals, the present invention solves its technical problem and is adopted the technical scheme that:
The production line of a kind of pilot scale culture plant stem cell, inoculates including pallet cleaning, drying line and culture medium fill freezing
Line；Described pallet cleaning, drying line includes the first conveyer belt, cleaning storehouse, blows sump and dryness storehouse, and described first conveyer belt is successively
Through described cleaning storehouse, described in blow sump and described dryness storehouse, described cleaning storehouse is provided with some row's shower nozzles, and described nozzle angle can
Regulation, described in blow sump in be provided with some air knives, described dryness storehouse blows sump closed butt joint with described, sets inside described dryness storehouse
There are some heat-generating pipes；Described culture medium fill freezing inoculation line includes the second conveyer belt, automatic filling device, cool chamber and inoculation
Machine, described automatic filling device, described cool chamber and described inoculation device be sequentially arranged on the direction of transfer of described second conveyer belt,
Described automatic filling device includes filling machine, agitator tank and instantaneous ultrahigh-temperature sterilization machine, and described cool chamber is located at by described filling machine
Entrance, described instantaneous ultrahigh-temperature sterilization machine is located between described filling machine and described agitator tank, inside described cool chamber successively
Being provided with high efficiency particulate air filter, blower fan and condenser, described cool chamber further respectively has water receiving tank, described inoculation device on internal two side
Being provided with hopper, described hopper is located at above described second conveyer belt.
As the optimal way of embodiment, described cleaning storehouse is provided with 3 row's shower nozzles, and the spray angle often arranging shower nozzle is different, institute
State cleaning storehouse and also include that movable water tank, described movable water tank are located at the side in described cleaning storehouse.
As the optimal way of embodiment, described in blow sump in be respectively provided with 4 air knives up and down.
As the optimal way of embodiment, described dryness storehouse internal temperature control is at 80-150 DEG C.
The width of pallet it is slightly larger than as the optimal way of embodiment, the entrance point of described dryness storehouse and the width of the port of export
Degree, also includes the door curtain made of cloth, and the door curtain made of cloth described in two is respectively arranged on the entrance point of described dryness storehouse and the port of export of described dryness storehouse.
As the optimal way of embodiment, described pallet cleaning, drying line also includes the first manually sale at reduced prices district and the first closing quotation
Workbench, the described first artificial sale at reduced prices district is located at the entrance point in described cleaning storehouse, and described first closing quotation workbench is located at described dry
The port of export in dry storehouse.
As the optimal way of embodiment, described filling machine is provided with 4 peristaltic pumps, and described peristaltic pump is located at described freezing
The entrance point in storehouse, described filling machine capacity is 50-500 milliliter, and described filling machine is provided with PLC and coordinates touch screen human-computer interface.
As the optimal way of embodiment, described in two, cool chamber is arranged in series, and described cool chamber top is provided with send ozone hole
And suction opeing, the entrance point of described cool chamber and the width of the port of export are slightly larger than the width of pallet, also include baffle plate, keep off described in two
Plate is respectively arranged on the entrance point of described cool chamber and the port of export of described cool chamber in the adjustable mode of height.
As the optimal way of embodiment, also include that fridge, described fridge are located at outside described cool chamber, and with described
Condenser connects.
As the optimal way of embodiment, described culture medium fill freezing inoculation line also includes that second manually sells at reduced prices Qu Ji
Two closing quotation workbench, the described second artificial sale at reduced prices district is located at the entrance point of described cool chamber, and described second closing quotation workbench is located at
The port of export of described inoculation device.
Owing to the production line of the present invention a kind of pilot scale culture plant stem cell have employed above-mentioned technical scheme, i.e. include
Pallet cleaning, drying line and culture medium fill freezing inoculation line；Described pallet cleaning, drying line include the first conveyer belt, clean storehouse,
Blow sump and dryness storehouse, described first conveyer belt sequentially pass through described cleaning storehouse, described in blow sump and described dryness storehouse, described clearly
Bring down stocks and be provided with some row's shower nozzles, described nozzle angle scalable, described in blow sump in be provided with some air knives, described dryness storehouse and institute
State and blow sump closed butt joint, inside described dryness storehouse, be provided with some heat-generating pipes；Described culture medium fill freezing inoculation line includes the
Two conveyer belts, automatic filling device, cool chamber and inoculation device, described automatic filling device, described cool chamber and described inoculation device
Being sequentially arranged on the direction of transfer of described second conveyer belt, described automatic filling device includes filling machine, agitator tank and superhigh temperature
Instantaneous sterilizing machine, described filling machine is located at the entrance of described cool chamber, and described filling machine is located at by described instantaneous ultrahigh-temperature sterilization machine
And between described agitator tank, it is sequentially provided with high efficiency particulate air filter, blower fan and condenser inside described cool chamber, inside described cool chamber
Further respectively having water receiving tank on two side, described inoculation device is provided with hopper, and described hopper is located at above described second conveyer belt；Make
Must the method have the advantages that
The present invention avoids the thinking of the fluid suspension culture of routine on the industrialized agribusiness of plant stem cell, have developed more
Simple efficient solid culture technical system.Production line of the present invention, uses 90 centimetres (length) * 60 centimetres (wide) * 3 centimetres (high)
Pallet 0.1 kilogram of cell of inoculation, through the quiescent culture of month, it is possible to produce 1 kilogram of fresh cell.The present invention is permissible
Cultivate tens thousand of basin cell the most simultaneously, greatly facilitate the production needs of scale, so this cultivating system is a kind of open
Cultivating system.
Accompanying drawing explanation
Fig. 1 is the pallet cleaning, drying line structure schematic diagram of the present invention；
Fig. 2 is the structural representation of the culture medium fill freezing inoculation line of the present invention；
Fig. 3 is the internal structure schematic diagram of the cool chamber of the present invention；
Fig. 4 is the structural representation of the inoculation device of the present invention.
Detailed description of the invention
Below in conjunction with the drawings and specific embodiments so that the invention will be further described.
As shown in Figure 1 to Figure 3, the production line of a kind of pilot scale culture plant stem cell, including pallet cleaning, drying line 1 He
Culture medium fill freezing inoculation line 2；Described pallet cleaning, drying line 1 include the first conveyer belt 11, clean storehouse 12, blow sump 13 and
Dryness storehouse 14, described first conveyer belt 11 sequentially pass through described cleaning storehouse 12, described in blow sump 13 and described dryness storehouse 14, described
Clean storehouse 12 and be provided with some row's shower nozzles 121, described nozzle angle scalable, described in blow sump in be provided with some air knives, described dry
Sump 13 closed butt joint is blown with described in dry storehouse 14, and described dryness storehouse 14 is internal is provided with some heat-generating pipes 141；Described culture medium fill
Freezing inoculation line 2 includes the second conveyer belt 21, automatic filling device 22, cool chamber 23 and inoculation device 24, and described auto-filling fills
Put 22, described cool chamber 23 and described inoculation device 24 to be sequentially arranged on the direction of transfer of described second conveyer belt 21, described automatically
It is described that filling apparatus 22 includes that filling machine 221, agitator tank 222 and instantaneous ultrahigh-temperature sterilization machine 223, described filling machine 221 are located at
The entrance of cool chamber 23, described instantaneous ultrahigh-temperature sterilization machine 223 is located between described filling machine 221 and described agitator tank 222, institute
State internal high efficiency particulate air filter 231, blower fan 232 and the condenser 233 of being sequentially provided with of cool chamber 23, two side, described cool chamber 23 inside
On further respectively have water receiving tank 234, described inoculation device 24 is provided with hopper 241, and described second conveyer belt 21 is located at by described hopper 241
Top.
As the optimal way of embodiment, described cleaning storehouse is provided with 3 row's shower nozzles, and the spray angle often arranging shower nozzle is different, institute
State cleaning storehouse and also include that movable water tank 121, described movable water tank are located at the side in described cleaning storehouse.
As the optimal way of embodiment, described in blow sump in be respectively provided with 4 air knives up and down.
As the optimal way of embodiment, described dryness storehouse 14 internal temperature control is at 80-150 DEG C.
The width of pallet it is slightly larger than as the optimal way of embodiment, the entrance point of described dryness storehouse and the width of the port of export
Degree, also includes the door curtain made of cloth, and the door curtain made of cloth described in two is respectively arranged on the entrance point of described dryness storehouse and the port of export of described dryness storehouse.
As the optimal way of embodiment, described pallet cleaning, drying line 1 also includes the first artificial sale at reduced prices district 15 and first
Closing quotation workbench 16, the described first artificial sale at reduced prices district 15 is located at the entrance point in described cleaning storehouse 12, described first closing quotation workbench
16 ports of export being located at described dryness storehouse 14.
As the optimal way of embodiment, described filling machine 221 is provided with 4 peristaltic pumps 2211, and described peristaltic pump is located at
The entrance point of described cool chamber 23, described filling machine 221 capacity is 50-500 milliliter, and described filling machine 221 is provided with PLC and coordinates tactile
Touch screen man machine interface.
As the optimal way of embodiment, described in two, cool chamber 23 is arranged in series, described cool chamber 23 top be provided with send smelly
Oxygen hole 234 and suction opeing 235, the entrance point of described cool chamber and the width of the port of export, slightly larger than the width of pallet, also include gear
Plate, baffle plate described in two is respectively arranged on entrance point and the port of export of described cool chamber of described cool chamber in the adjustable mode of height
On.
As the optimal way of embodiment, also include that fridge 25, described fridge 25 are located at outside described cool chamber 23, and
It is connected with described condenser 233.
As the optimal way of embodiment, described culture medium fill freezing inoculation line 2 also includes the second artificial sale at reduced prices district 26
And the second closing quotation workbench 27, described second manually sale at reduced prices district 26 be located at the entrance point of described cool chamber 23, described second closing quotation
The port of export of described inoculation device 24 is located at by workbench 27.
The vinyl disc that pallet is resistance to 160-170 DEG C of high temperature used in this production line, is similar to after the culture medium heating of dress in dish
Water preparation, is similar to g., jelly-like under room temperature.The door cleaning storehouse, dryness storehouse and cool chamber involved by this production line can be not
Close during work, open again during work, so close door when not working and keep a relatively clean state.Whole piece
Production line is all to be moved along by dish by conveyer belt, and an emergency stop switch installed by each section of conveyer belt, is arranged on ratio more prominent
Place, operation when facilitating emergency.
The flow process of this pallet cleaning, drying line is as follows:
1, manually sale at reduced prices: manually selling at reduced prices district first, artificial left-hand thread has struck off the pallet of culture medium, do not stacked.
2, cleaning: cleaning storehouse, arranging 3 row's shower nozzles, the angle often arranging shower nozzle is different: row's shower nozzle is vertical, row's shower nozzle
Always basin direction tilts, and row's shower nozzle, to going to basin direction to tilt, ensures to clean comprehensively, and nozzle angle is adjustable.
3, air-dry: in hot blast blows sump, be respectively arranged with 4 air knives up and down, to blow the oarse-grained globule off.
4, dry: at dryness storehouse, dryness storehouse with blow sump closed butt joint, the wind in dryness storehouse is in inner loop, and temperature can
To reach 80-150 DEG C, two ends are left the import and export just permission dish of dish for and are passed through, and the band door curtain made of cloth.
5, closing quotation: in the first closing quotation workbench, platform band gradient of closing；When dish is stacked to certain altitude, manually take away,
Take away sterilizing between sterilizing.
The flow process of this culture medium fill freezing inoculation line is as follows:
1, manually sale at reduced prices: manually selling at reduced prices district second, the pallet after sterilizing is placed in front, does not stacks.
2, auto-filling: automatic filling device is provided with the filling machine of 4 peristaltic pumps, fill capacity 50-500 milliliter, program
Control to coordinate touch screen human-computer interface for PLC；It is additionally equipped with 2 1.5 tons of heating and stirring tank, 11 ton of agitator tank and 2 superelevation
Temperature instantaneous sterilizing machine, is respectively used to the configuration of culture medium, storage and sterilizing.
3, freezing: being provided with 2 Leng Dong storehouses, the side door of cool chamber can arbitrarily regulate, and two ends are left the import and export of dish for and just permitted
Being permitted dish to pass through, it is adjustable that doorway, two ends installs baffle plate height additional；2 holes of cool chamber middle device, one is the convulsion of a diameter of 15cm
Mouthful, be used for taking ozone away, one be send into ozone a diameter of 5cm of carrying out sterilizing send ozone hole；In order to reduce condensed water, cold
Freeze storehouse and use plastic plate, side below condenser employing；Both sides, Leng Dong storehouse, equipped with connecing cold water reservoir, tilt to install, below afterbody
Meet flexible pipe draining, wind speed≤0.4 m/s.It is configured with two concatenation fridges, is arranged on outside cool chamber.
4, inoculation: be provided with a two-way inoculation device, as shown in Figure 4, described inoculation device 24 include hopper 241, support 242,
Pneumatic vibrator 243 and air blowing device (not shown), two the second conveyer belts 21 being mutually juxtaposed are located at below support 242, described
Support 242 side is provided with height regulating rod 244, and two hoppers 241 are the most slidably located on support 241, and two is pneumatic
Vibrator 243 is respectively arranged on two hoppers 241, facilitates the smooth and easy whereabouts of seed, two air blowing devices (to use rustless steel square tube fluting to do
Air knife) it is respectively arranged on one end of support 242；The seed using this inoculation device to connect is soft, size 1cm-5cm；Fill above conveyer belt
There is fender plate 245, it is simple to material is uniform.
5, closing quotation: at the second closing quotation workbench, be provided with the estrade of no-power drum, it is simple to packing, turn after plate is packed
Cultivate between cultivating.
Our company has carried out a series of research and development, by adding antibacterial success conventional in food industry in the medium
Establish a set of efficient open culturing system, pollution problem also will not occur under the conditions of the cleanliness factor of 100,000 grades.Newly-built
The cleanliness factor of factory is set in ten thousand grades, and local reaches hundred grades so that this system successfully operates.The production line of the present invention makes rule
Modelling is cultivated plant cell and is become more efficient, is easier to, and cost is lower.The production line of the present invention is exactly exclusively for solid culture skill
Art realizes plant layout and sets up.
All deformation that those of ordinary skill in the art can directly derive from the disclosure of invention or associate, all should
It is considered protection scope of the present invention.
Claims (10)
1. the production line of a pilot scale culture plant stem cell, it is characterised in that: include pallet cleaning, drying line and culture medium
Fill freezing inoculation line；Described pallet cleaning, drying line includes the first conveyer belt, cleans storehouse, blows sump and dryness storehouse, and described the
One conveyer belt sequentially pass through described cleaning storehouse, described in blow sump and described dryness storehouse, described cleaning storehouse is provided with some row's shower nozzles, institute
State nozzle angle scalable, described in blow sump in be provided with some air knives, described dryness storehouse blows sump closed butt joint with described, described
Some heat-generating pipes it are provided with inside dryness storehouse；Described culture medium fill freezing inoculation line include the second conveyer belt, automatic filling device,
Cool chamber and inoculation device, described automatic filling device, described cool chamber and described inoculation device are sequentially arranged in described second conveyer belt
Direction of transfer on, described automatic filling device includes filling machine, agitator tank and instantaneous ultrahigh-temperature sterilization machine, and described filling machine sets
In the entrance of described cool chamber, described instantaneous ultrahigh-temperature sterilization machine is located between described filling machine and described agitator tank, described cold
Freezing and be sequentially provided with high efficiency particulate air filter, blower fan and condenser inside storehouse, described cool chamber further respectively has water receiving on internal two side
Groove, described inoculation device is provided with hopper, and described hopper is located at above described second conveyer belt.
The production line of a kind of pilot scale culture plant stem cell the most as claimed in claim 1, it is characterised in that: described cleaning storehouse
Being provided with 3 row's shower nozzles, the spray angle often arranging shower nozzle is different, and described cleaning storehouse also includes movable water tank, described movable water tank
It is located at the side in described cleaning storehouse.
The production line of a kind of pilot scale culture plant stem cell the most as claimed in claim 1, it is characterised in that blow sump described in:
It is respectively provided with 4 air knives the most up and down.
The production line of a kind of pilot scale culture plant stem cell the most as claimed in claim 1, it is characterised in that: described dryness storehouse
Internal temperature control is at 80-150 DEG C.
The production line of a kind of pilot scale culture plant stem cell the most as claimed in claim 1, it is characterised in that: described dryness storehouse
Entrance point and the width of the port of export slightly larger than the width of pallet, also include the door curtain made of cloth, the door curtain made of cloth described in two is respectively arranged on described being dried
On the entrance point in storehouse and the port of export of described dryness storehouse.
The production line of a kind of pilot scale culture plant stem cell the most as claimed in claim 1, it is characterised in that: described pallet is clear
Wash drying line and also include that described cleaning storehouse is located at by the first artificial sale at reduced prices district and the first closing quotation workbench, the described first artificial sale at reduced prices district
Entrance point, the port of export of described dryness storehouse is located at by described first closing quotation workbench.
The production line of a kind of pilot scale culture plant stem cell the most as claimed in claim 1, it is characterised in that: described filling machine
Being provided with 4 peristaltic pumps, described peristaltic pump is located at the entrance point of described cool chamber, and described filling machine capacity is 50-500 milliliter,
Described filling machine is provided with PLC and coordinates touch screen human-computer interface.
The production line of a kind of pilot scale culture plant stem cell the most as claimed in claim 1, it is characterised in that: freezing described in two
Storehouse is arranged in series, and described cool chamber top is provided with send ozone hole and suction opeing, the entrance point of described cool chamber and the width of the port of export
Degree, slightly larger than the width of pallet, also includes baffle plate, and baffle plate described in two is respectively arranged on described cool chamber in the adjustable mode of height
On the port of export of entrance point and described cool chamber.
The production line of a kind of pilot scale culture plant stem cell the most as claimed in claim 1, it is characterised in that: also include freezing
Machine, described fridge is located at outside described cool chamber, and is connected with described condenser.
The production line of a kind of pilot scale culture plant stem cell the most as claimed in claim 1, it is characterised in that: described cultivation
Base fill freezing inoculation line also includes that institute is located at by the second artificial sale at reduced prices district and the second closing quotation workbench, the described second artificial sale at reduced prices district
Stating the entrance point of cool chamber, the port of export of described inoculation device is located at by described second closing quotation workbench.
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| CN201610381074.3A CN106047704B (en) | 2016-06-01 | 2016-06-01 | A kind of production line of pilot scale culture plant stem cell |
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| CN201610381074.3A CN106047704B (en) | 2016-06-01 | 2016-06-01 | A kind of production line of pilot scale culture plant stem cell |
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| CN106047704A true CN106047704A (en) | 2016-10-26 |
| CN106047704B CN106047704B (en) | 2018-05-29 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108504566A (en) * | 2017-02-27 | 2018-09-07 | 厦门鹭港兆康生物科技有限公司 | It is a kind of to generate producing line without change different homology plant stem cell normalized quantity |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104207308A (en) * | 2014-07-30 | 2014-12-17 | 刘会平 | Method and system for removing seed coats in walnuts through alkali-free method pneumatic blow injection |
| CN104534845A (en) * | 2014-12-19 | 2015-04-22 | 山东圣琪生物有限公司 | Continuous vacuum belt type drying device |
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| CN102174395A (en) * | 2011-01-30 | 2011-09-07 | 中国科学院广州生物医药与健康研究院 | Automation augmentation and culture system of induced pluripotent stem cells |
| CN102250767A (en) * | 2011-06-28 | 2011-11-23 | 江苏省北科生物科技有限公司 | Digitalized automatic production system for mesenchymal stem cells of umbilical cord |
| CN104207308A (en) * | 2014-07-30 | 2014-12-17 | 刘会平 | Method and system for removing seed coats in walnuts through alkali-free method pneumatic blow injection |
| CN104534845A (en) * | 2014-12-19 | 2015-04-22 | 山东圣琪生物有限公司 | Continuous vacuum belt type drying device |
| CN105349422A (en) * | 2015-12-03 | 2016-02-24 | 遵义医学院 | Plant cell suspension culture device |
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| CN108504566A (en) * | 2017-02-27 | 2018-09-07 | 厦门鹭港兆康生物科技有限公司 | It is a kind of to generate producing line without change different homology plant stem cell normalized quantity |
| CN108504566B (en) * | 2017-02-27 | 2023-12-12 | 深圳市赢联丰生物科技有限公司 | Standardized mass production line for non-variant homologous plant stem cells |
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| CN106047704B (en) | 2018-05-29 |
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