CN108504566B - Standardized mass production line for non-variant homologous plant stem cells - Google Patents
Standardized mass production line for non-variant homologous plant stem cells Download PDFInfo
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- CN108504566B CN108504566B CN201710107997.4A CN201710107997A CN108504566B CN 108504566 B CN108504566 B CN 108504566B CN 201710107997 A CN201710107997 A CN 201710107997A CN 108504566 B CN108504566 B CN 108504566B
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- Prior art keywords
- production line
- stem cells
- plant stem
- laminar flow
- mass production
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 51
- 241000196324 Embryophyta Species 0.000 title claims abstract description 37
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 35
- 230000001954 sterilising effect Effects 0.000 claims abstract description 36
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 34
- 238000003306 harvesting Methods 0.000 claims abstract description 20
- 230000007306 turnover Effects 0.000 claims abstract description 20
- 238000001816 cooling Methods 0.000 claims abstract description 19
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 16
- 210000004027 cell Anatomy 0.000 claims abstract description 15
- 238000007789 sealing Methods 0.000 claims abstract description 12
- 239000005341 toughened glass Substances 0.000 claims abstract description 11
- 239000012528 membrane Substances 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims 1
- 238000005496 tempering Methods 0.000 claims 1
- 241000894006 Bacteria Species 0.000 abstract description 7
- 238000002955 isolation Methods 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 238000001914 filtration Methods 0.000 abstract description 3
- 238000007599 discharging Methods 0.000 abstract 1
- 238000005457 optimization Methods 0.000 description 7
- 230000004888 barrier function Effects 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000000442 meristematic effect Effects 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000008121 plant development Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/18—Rollers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
Abstract
The invention discloses a standardized mass production line of non-variant homologous plant stem cells, which comprises a culture dish sterilization room, a cooling laminar flow hood, a cell harvesting and feeding platform, a turnover dish harvesting device, a plate stacking frame, a laminar flow isolator, a sterilization room, a hanging rail, a hydrogen peroxide charging port, a primary filter, a high-efficiency filter, a flow equalizing film, an isolation door, a cover plate, a toughened glass sealing door, a fresh air port, a cylinder turnover dish, a telescopic cylinder push rod, a lifter and a cylinder conveying roller. The beneficial effects of the invention are as follows: the culture plate is conveyed by adopting a hanging rail in a sterilizing room of the culture plate, and the cooling laminar flow hood adopts a multi-stage filter for filtering, so that the plant stem cells are prevented from being infected by bacteria during culture production, and the production quality of the plant stem cells is improved; the turnover device has the advantages that the turnover device is turned over by 180 degrees at regular time, and the lifter is used for automatically pushing, harvesting and discharging products, so that high automation of a production line is ensured, and the production efficiency is improved; the production line is convenient to use, and each component is flexible to connect.
Description
Technical Field
The invention relates to a production line, in particular to a standardized mass production line of non-variant homologous plant stem cells, and belongs to the technical field of plant stem cell production and application.
Background
Plant stem cells, also called meristematic tissue, are classified according to their location into the apical and lateral meristematic tissue, relative to the mature tissue that has differentiated and matured in plants. The non-variant homologous plant stem cells are important items in scientific research, are beneficial to people to know the composition characteristics of different plants, the promotion points of plant growth and development and the like, and the non-variant homologous plant stem cell standardized mass production line is an important plant stem cell production process line in life, and has indispensable effects on mass production and research of plant stem cells. As a plant stem cell production line which is particularly important in life, the existing standard mass production line of the plant stem cells without mutation homology has the defects of easy bacteria contamination, low automation performance of the production line and poor bacteria isolation in the cell production process, so the standard mass production line of the plant stem cells without mutation homology is provided for solving the problems.
Disclosure of Invention
The invention aims to solve the problems and provide a standardized mass production line of non-variant homologous plant stem cells.
The invention realizes the aim through the following technical scheme, and the standardized mass production line of the non-variant homologous plant stem cells comprises a culture dish sterilization room and is characterized in that: a cooling laminar flow cover is arranged on one side of the culture dish sterilization room, a cell harvesting and feeding platform is arranged above the cooling laminar flow cover, a tray turnover device is arranged below the cell harvesting and feeding platform, a tray stacking frame is arranged below the tray turnover device, a laminar flow isolator is arranged on one side of the tray stacking frame, a sterilization room is arranged inside the cell sterilization room, a hanging rail is arranged inside the sterilization room, a hydrogen peroxide charging port is arranged above the cooling laminar flow cover, a primary filter is arranged on one side of the hydrogen peroxide charging port, and the primary filter below sets up high-efficient filter, high-efficient filter below sets up the flow equalizing membrane, just laminar flow isolator one side sets up the division gate, the division gate top sets up the cover plate, just set up toughened glass sealing door on the cover plate, toughened glass sealing door below sets up the fresh air inlet, just the inside cylinder turn-over dish that sets up of turn-over dish harvesting apparatus, cylinder turn-over dish one side sets up flexible cylinder push rod, just flexible cylinder push rod below sets up the lift, lift one side sets up feed cylinder conveying cylinder.
Preferably, in order to make the production line produce plant stem cells more efficiently and sterilize, avoid the bacterial contamination performance higher, a plurality of sterilization rooms are arranged inside the culture dish sterilization room.
Preferably, the hanging rail has a U-shaped structure in order to prevent bacteria from being stained when the culture dish is conveyed and to enable the conveying efficiency to be higher.
Preferably, in order to enable the cylinder turner to have enough power to perform timing turnover, the automation level of the production line is improved, and the telescopic cylinder push rod is movably connected with the cylinder turner.
Preferably, in order to ensure that the sealing performance of the laminar flow isolator is higher, the production line is ensured not to be infected with bacteria, the production quality of plant stem cells is improved, and a plurality of toughened glass sealing doors are arranged on the laminar flow isolator.
Preferably, in order to make the cell tray have enough space for lifting, pushing and harvesting by a lifter after operation and transportation, a cell tray placing platform is arranged above the lifter.
Preferably, in order to enable the production line to ventilate the plant stem cells and improve the growth activity of the plant stem cells, a plurality of fresh air inlets are arranged on the laminar flow isolator.
Preferably, in order to make the cooling laminar flow cover more firm in operation, the cooling laminar flow cover below sets up the toper base.
The beneficial effects of the invention are as follows: the culture tray sterilization room of the production line adopts a plurality of sterilization room assembly designs, a suspension rail is adopted in the sterilization room to carry out transportation and circulation of the culture tray, a cooling laminar flow cover adopts a multistage filter to carry out sterilization and filtration, and a laminar flow isolator is provided with a plurality of toughened glass sealing doors, so that plant stem cells are not infected during culture production, the bacteria isolation performance of the production line is higher, and the production quality of the plant stem cells is improved; the production line turnover plate harvest adopts the cylinder turnover plate and performs 180-degree turnover at regular time, and the lifter is utilized to automatically push, harvest and discharge products, so that the high automation of the production line is ensured, and the production efficiency is improved; the production line is convenient to use, and each component is flexible to connect.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present invention.
FIG. 2 is a schematic view showing the structure of the sterilization chamber of the culture dish of the present invention.
FIG. 3 is a schematic view of a cooling laminar flow hood according to the present invention.
FIG. 4 is a schematic view of a laminar flow isolator according to the present invention.
FIG. 5 is a schematic view of a tray harvester according to the present invention.
In the figure: 1. a culture dish sterilization room; 2. a cooling laminar flow hood; 3. a cell harvesting and feeding platform; 4. a tray-turning harvesting device; 5. a tray stacking frame; 6. a laminar flow isolator; 7. a sterilizing room; 8. a hanging rail; 9. a hydrogen peroxide charging port; 10. a primary filter; 11. a high-efficiency filter; 12. a flow equalizing membrane; 13. an isolation door; 14. a cover plate; 15. tempered glass sealing door; 16. a new wind gap; 17. a cylinder overturning plate; 18. a telescopic cylinder push rod; 19. a lifter; 20. the feed cylinder conveys the cylinder.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-5, a standardized mass production line for non-variant homologous plant stem cells comprises a culture dish sterilization room 1, wherein a cooling laminar flow hood 2 is arranged on one side of the culture dish sterilization room 1, a cell harvesting and feeding platform 3 is arranged above the cooling laminar flow hood 2, a turnover plate harvesting device 4 is arranged below the cell harvesting and feeding platform 3, a barrier plate frame 5 is arranged below the turnover plate harvesting device 4, a laminar flow isolator 6 is arranged on one side of the barrier plate frame 5, a sterilization room 7 is arranged inside the cell sterilization room 1, a hanging rail 8 is arranged inside the sterilization room 7, a hydrogen peroxide charging port 9 is arranged above the cooling laminar flow hood 2, a primary filter 10 is arranged on one side of the hydrogen peroxide charging port 9, an efficient filter 11 is arranged below the primary filter 10, a flow equalizing membrane 12 is arranged below the efficient filter 11, a separation door 13 is arranged on one side of the laminar flow isolator 6, a cover plate 14 is arranged above the separation door 13, a toughened glass sealing door 15 is arranged on the cover plate 14, a new wind port 16 is arranged below the toughened glass sealing door 15, a cylinder turnover plate 17 is arranged inside the turnover plate device 4, a telescopic push rod 18 is arranged on one side of the cylinder push rod 18, a telescopic push rod 19 is arranged below the telescopic push rod 18, and a telescopic cylinder 19 is arranged on one side of the telescopic push rod and a lifter 19 is arranged.
As a technical optimization scheme of the invention, a plurality of sterilization rooms 7 are arranged in the culture dish sterilization room 1.
As a technical optimization scheme of the invention, the suspension rail 8 is of a U-shaped structure.
As a technical optimization scheme of the invention, a telescopic cylinder push rod 18 is movably connected with a cylinder turnplate 17.
As a technical optimization scheme of the invention, a plurality of toughened glass sealing doors 15 are arranged on the laminar flow isolator 6.
As a technical optimization scheme of the invention, a cell tray placing platform is arranged above the lifter 19.
As a technical optimization scheme of the invention, a plurality of fresh air ports 16 are arranged on the laminar flow isolator 6.
As a technical optimization scheme of the invention, a conical base is arranged below the cooling laminar flow hood 2.
When the cultivation box is used, firstly, the position of the whole production line is fixed, secondly, when the cultivation box is used, a plurality of sterilization rooms 7 in a cultivation plate sterilization room 1 sterilize and convey cultivation plates, so that the production line is faster in production efficiency of plant stem cells, higher in sterilization and bacteria-infection-preventing performance, the cultivation plates are conveyed and circulated through a hanging rail 8 in the sterilization room 7, and sterilization and filtration are carried out through a primary filter 10 and a high-efficiency filter 11 of a cooling laminar flow hood 2, so that the plant stem cells are prevented from being infected with bacteria during cultivation production, and are sealed through a plurality of toughened glass sealing doors 15 on a laminar flow isolator 6, the bacteria-isolation performance of the production line is higher, and the production quality of the plant stem cells is improved; the telescopic cylinder push rod 18 pushes the cylinder turnplate 17 to turn 180 degrees at regular time, and the lifter 19 automatically pushes the product to harvest and discharge, so that the high automation of the production line is ensured, and the production efficiency is improved.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.
Claims (8)
1. The standardized mass production line of the non-variant homologous plant stem cells comprises a culture dish sterilization room (1), and is characterized in that: the utility model discloses a novel air conditioner, which is characterized in that a cooling laminar flow cover (2) is arranged on one side of a culture dish sterilization room (1), a cell harvesting and feeding platform (3) is arranged above the cooling laminar flow cover (2), a turnover dish harvesting device (4) is arranged below the cell harvesting and feeding platform (3), a dish stacking frame (5) is arranged below the turnover dish harvesting device (4), a laminar flow isolator (6) is arranged on one side of the dish stacking frame (5), a sterilization room (7) is arranged inside the culture dish sterilization room (1), a hanging rail (8) is arranged inside the sterilization room (7), a hydrogen peroxide charging port (9) is arranged above the cooling laminar flow cover (2), a primary filter (10) is arranged on one side of the hydrogen peroxide charging port (9), a high-efficiency filter (11) is arranged below the primary filter (10), a membrane (12) is arranged below the high-efficiency filter, a separation door (13) is arranged on one side of the laminar flow isolator (6), a cover plate (14) is arranged above the separation door (13), a glass tempering door (15) is arranged on the cover plate (14), a new air conditioner (16) is arranged below the turnover dish (17), the telescopic cylinder pushing rod (18) is arranged on one side of the cylinder turning plate (17), the telescopic cylinder pushing rod (18) pushes the cylinder turning plate (17) to turn 180 degrees regularly, the lifter (19) is arranged below the telescopic cylinder pushing rod (18), and the charging barrel conveying roller (20) is arranged on one side of the lifter (19).
2. The standardized mass production line for non-variant and homologous plant stem cells according to claim 1, wherein: a plurality of sterilization rooms (7) are arranged in the culture dish sterilization room (1).
3. The standardized mass production line for non-variant and homologous plant stem cells according to claim 1, wherein: the hanging rail (8) is of a U-shaped structure.
4. The standardized mass production line for non-variant and homologous plant stem cells according to claim 1, wherein: the telescopic cylinder push rod (18) is movably connected with the cylinder turnplate (17).
5. The standardized mass production line for non-variant and homologous plant stem cells according to claim 1, wherein: a plurality of toughened glass sealing doors (15) are arranged on the laminar flow isolator (6).
6. The standardized mass production line for non-variant and homologous plant stem cells according to claim 1, wherein: a cell tray placing platform is arranged above the lifter (19).
7. The standardized mass production line for non-variant and homologous plant stem cells according to claim 1, wherein: a plurality of fresh air ports (16) are arranged on the laminar flow isolator (6).
8. The standardized mass production line for non-variant and homologous plant stem cells according to claim 1, wherein: and a conical base is arranged below the cooling laminar flow hood (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710107997.4A CN108504566B (en) | 2017-02-27 | 2017-02-27 | Standardized mass production line for non-variant homologous plant stem cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710107997.4A CN108504566B (en) | 2017-02-27 | 2017-02-27 | Standardized mass production line for non-variant homologous plant stem cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108504566A CN108504566A (en) | 2018-09-07 |
| CN108504566B true CN108504566B (en) | 2023-12-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710107997.4A Active CN108504566B (en) | 2017-02-27 | 2017-02-27 | Standardized mass production line for non-variant homologous plant stem cells |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1389565A (en) * | 2002-07-08 | 2003-01-08 | 徐如祥 | Culture process of human nerve stem cell |
| WO2008107695A1 (en) * | 2007-03-07 | 2008-09-12 | Stem Cell Sciences (Uk) Ltd | Automated culture of stem cells |
| CN102061260A (en) * | 2010-12-01 | 2011-05-18 | 汪华 | Medical automatic cell production device and production method thereof |
| JP2011167405A (en) * | 2010-02-19 | 2011-09-01 | Kawasaki Heavy Ind Ltd | Isolator, automatic cell culture device, and sterilization process of the isolator |
| CN106047704A (en) * | 2016-06-01 | 2016-10-26 | 厦门鹭港兆康生物科技有限公司 | Production line for culturing plant stem cells on large scale |
| CN106383503A (en) * | 2016-10-20 | 2017-02-08 | 广东省心血管病研究所 | Stem cell preparation production process transfer control system based on IOT |
| CN206521488U (en) * | 2017-02-27 | 2017-09-26 | 厦门鹭港兆康生物科技有限公司 | It is a kind of to produce producing line without change different homology plant stem cell normalized quantity |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100151564A1 (en) * | 2008-12-12 | 2010-06-17 | Beebe David J | Biological Work Station |
-
2017
- 2017-02-27 CN CN201710107997.4A patent/CN108504566B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1389565A (en) * | 2002-07-08 | 2003-01-08 | 徐如祥 | Culture process of human nerve stem cell |
| WO2008107695A1 (en) * | 2007-03-07 | 2008-09-12 | Stem Cell Sciences (Uk) Ltd | Automated culture of stem cells |
| JP2011167405A (en) * | 2010-02-19 | 2011-09-01 | Kawasaki Heavy Ind Ltd | Isolator, automatic cell culture device, and sterilization process of the isolator |
| CN102061260A (en) * | 2010-12-01 | 2011-05-18 | 汪华 | Medical automatic cell production device and production method thereof |
| CN106047704A (en) * | 2016-06-01 | 2016-10-26 | 厦门鹭港兆康生物科技有限公司 | Production line for culturing plant stem cells on large scale |
| CN106383503A (en) * | 2016-10-20 | 2017-02-08 | 广东省心血管病研究所 | Stem cell preparation production process transfer control system based on IOT |
| CN206521488U (en) * | 2017-02-27 | 2017-09-26 | 厦门鹭港兆康生物科技有限公司 | It is a kind of to produce producing line without change different homology plant stem cell normalized quantity |
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| CN108504566A (en) | 2018-09-07 |
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Effective date of registration: 20231025 Address after: 518000, 401, No. 36 Dadongmen Street, Xinlan Community, Guanlan Street, Longhua District, Shenzhen City, Guangdong Province Applicant after: Shenzhen Yinglianfeng Biotechnology Co.,Ltd. Address before: 1st and 4th floors of Building 11, Biopharmaceutical General Factory, No. 2020 Wengjiao West Road, Haicang District, Xiamen City, Fujian Province, 361026 Applicant before: XIAMEN AMOY HERON BIOTECHNOLOGY CO.,LTD. |
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