CN220846136U - Device for preventing mildew spores from diffusing in mould culture - Google Patents
Device for preventing mildew spores from diffusing in mould culture Download PDFInfo
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- CN220846136U CN220846136U CN202322571817.1U CN202322571817U CN220846136U CN 220846136 U CN220846136 U CN 220846136U CN 202322571817 U CN202322571817 U CN 202322571817U CN 220846136 U CN220846136 U CN 220846136U
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- mould
- diffusing
- bottom box
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- 239000010410 layer Substances 0.000 claims abstract description 23
- 239000011229 interlayer Substances 0.000 claims abstract description 19
- 238000005192 partition Methods 0.000 claims abstract description 6
- 238000009792 diffusion process Methods 0.000 claims abstract description 4
- 238000009423 ventilation Methods 0.000 claims abstract description 4
- 238000005260 corrosion Methods 0.000 claims description 5
- 230000007797 corrosion Effects 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 125000006850 spacer group Chemical group 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims 2
- 238000002474 experimental method Methods 0.000 abstract description 3
- 230000002452 interceptive effect Effects 0.000 abstract description 2
- 230000001580 bacterial effect Effects 0.000 description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000003912 environmental pollution Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Chemical compound Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- FYZYXYLPBWLLGI-AUOPOVQUSA-N Genipin 1-beta-gentiobioside Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O)O[C@@H]1OC=C([C@@H]2[C@H]1C(=CC2)CO)C(=O)OC)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O FYZYXYLPBWLLGI-AUOPOVQUSA-N 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960002233 benzalkonium bromide Drugs 0.000 description 1
- KHSLHYAUZSPBIU-UHFFFAOYSA-M benzododecinium bromide Chemical compound [Br-].CCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1 KHSLHYAUZSPBIU-UHFFFAOYSA-M 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229920001903 high density polyethylene Polymers 0.000 description 1
- 239000004700 high-density polyethylene Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011076 safety test Methods 0.000 description 1
- 238000006748 scratching Methods 0.000 description 1
- 230000002393 scratching effect Effects 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
Abstract
The utility model relates to a device for preventing mildew spores from diffusing in mould culture, belonging to the technical field of laboratory equipment. The interlayer frame is any layered frame with a multi-layer structure, and through arranging the interlayer frame, a culture box can be respectively placed on each interlayer of the interlayer frame, so that the use space of the culture box is saved. The culture box comprises a top cover and a bottom box; a partition plate for separating and placing the culture dishes is arranged in the bottom box; the top cover is covered on the bottom box, colony observation windows are arranged on the top cover and are opposite to the culture dishes placed in the bottom box one by one, and the middle part of the top cover is provided with ventilation holes; the utility model can effectively prevent mildew spore diffusion from interfering with the mould counting experiment, can directly observe the growth condition of a plurality of flat plate colonies at one time, improves the accuracy of mould counting, improves the working efficiency of experimenters, and saves the space of an incubator.
Description
Technical Field
The utility model belongs to the technical field of laboratory equipment, and particularly relates to a device for preventing mildew spores from diffusing in mould culture.
Background
GB 4789.15-2016 "food safety national Standard food microbiology test mould and Yeast count" prescribes "after agar solidification, plates were placed right after, incubated in an incubator at 28 ℃ + -1 ℃, and the results of incubation to 5d were observed and recorded. The new standard compared with GBGB 4789.15-2010 "food safety national Standard food microbiology test mould and Yeast count" modifies the plate culture from inverted culture to the normal culture. The orthoculturing is a means to avoid the formation of secondary microcolonies by reversing the plate upside down during repeated observations, resulting in the diffusion of mold spores. However, in the actual culture process, after the mold is cultivated in the normal place, the mold colony grows on a blank plate compared with the prior inverted culture, and the mold colony is regulated in a new standard; "if there is colony growth on the blank control plate, the detection effect is ineffective", which indicates that there is mould spore pollution. CNAS-CL01-A001-2022 application of the detection and calibration laboratory Capacity approval guidelines in the field of microbiological detection specifically requires "when detecting mould in a sample, proper measures are taken to control the spread of spores in air". There are a number of reasons for this, with incubator environmental pollution being the most common. Incubator environmental pollution: the special mould incubator is adopted for cultivating the mould, most mould incubators adopt hot air circulation, the equipment is provided with a fan, and the hot air flow in the incubator circularly flows. The space of the incubator is provided with residual mould spores which fly away along with the airflow. The top cover and the bottom dish of the culture dish are provided with a certain gap, especially the gap of the glass plate is larger, spores can fly upwards to fall on the culture medium of the plate through the gap in the normal culture, so that mould spores are polluted, and most polluted colonies grow on the edge of the plate. The spores of the mould are very difficult to remove, and although the incubator is sterilized periodically, because the mould is continuously cultured for a long time, new pollution sources can be introduced into each culture, and the introduction of environmental pollution is difficult to avoid.
According to GB 4789.15-2016 national Standard food microbiology for food safety test mould and Yeast counts, mould counts require 2-3 adapted dilutions (2 plates per dilution) and blank control, 6-8 plates, and the results of the culture to 5d are observed and recorded, which requires experimenters to observe the plates every day and cannot directly culture to 5d counts because of the mould specificity, a large number of spores are generated during the culture, resulting in the spread of the whole plate. Thus, at the beginning of day 2 after incubation, the plates were observed and representative colonies were counted until day 5 of incubation. The inspector takes time to take out each plate every day for observation.
Disclosure of Invention
In order to overcome the problems in the background art, the utility model provides a device for preventing mildew spores from diffusing in mould culture, and the culture box and the interlayer frame are arranged to effectively prevent the mildew spores from diffusing to interfere with mould counting experiments, so that the growth condition of a plurality of flat plate colonies can be directly observed at one time, the accuracy of mould counting is improved, and the working efficiency of experimental staff is improved.
In order to achieve the above purpose, the utility model is realized by the following technical scheme:
The device for preventing mildew spores from diffusing in the mould culture comprises a culture box; the culture box comprises a top cover and a bottom box; a partition plate for separating and placing the culture dishes is arranged in the bottom box; the top cover is covered on the bottom box, colony observation windows are arranged on the top cover and are opposite to the culture dishes placed in the bottom box one by one, and ventilation holes are formed in the middle of the top cover.
Preferably, the top cover comprises a top panel and side plates arranged on two sides of the top panel, and the side plates cover the outer side of the bottom box.
Preferably, the end angle of the side plate is an arc angle.
Preferably, the device further comprises a spacer frame; the interlayer frame is of a multi-layer structure, and the layer height is larger than the height of the culture box.
Preferably, the bottom box is a rectangular box; the division board sets up one, and the division board separates the end box into two spaces along the length direction's of end box central line.
Preferably, the eight corners in the square bottom box are respectively provided with round corners.
Preferably, four dishes are placed in each space.
Preferably, the culture box and the interlayer frame are made of corrosion-resistant materials.
Preferably, the interlayer frame is provided with two layers, namely a bottom layer and a top layer, and a culture box is respectively arranged on the bottom layer and the top layer.
The utility model has the beneficial effects that:
The utility model can effectively prevent mildew spore diffusion from interfering with the mildew counting experiment, and can directly observe the growth condition of a plurality of flat plate colonies at one time, thereby improving the accuracy of the mildew counting and the working efficiency of experimenters; the interlayer frame can be used for placing a plurality of culture boxes, so that the space utilization rate of the culture boxes is improved.
Drawings
FIG. 1 is a schematic view of the structure of a culture cassette of the present utility model;
FIG. 2 is a top view of FIG. 1;
FIG. 3 is a schematic view of the top cover structure of the present utility model;
FIG. 4 is a schematic view of the structure of the bottom case of the present utility model;
FIG. 5 is a schematic view of the structure of the spacer frame of the present utility model;
In the figure, 1-top cover, 11-top panel, 12-side plate, 2-bottom box, 3-partition plate, 4-colony observation window, 5-ventilation hole, 6-round angle, 7-bottom layer and 8-top layer.
Description of the embodiments
In order to make the objects, technical solutions and advantageous effects of the present utility model more apparent, preferred embodiments of the present utility model will be described in detail below with reference to the accompanying drawings, so as to facilitate understanding of the skilled person.
In the description of the present utility model, unless otherwise indicated, the terms "inner," "upper," "lower," and the like refer to an orientation or state relationship based on that shown in the drawings, for convenience of description and simplicity of description, and do not necessarily indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the utility model.
In the description of the present utility model, it should be noted that, unless explicitly stated and limited otherwise, the terms "mounted," "connected," and "provided" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium. To one of ordinary skill in the art, the specific meaning of the terms in the present utility model is understood as appropriate
As shown in FIGS. 1-4, the device for preventing mildew spores from diffusing in mould culture comprises a culture box and a interlayer frame.
The interlayer frame is any layered frame with a multi-layer structure, and through arranging the interlayer frame, a culture box can be respectively placed on each interlayer of the interlayer frame, so that the use space of the culture box is saved, and the space utilization rate of the culture box is improved. The interlayer frame of this embodiment sets up two-layer (like fig. 4), is bottom 7 and top layer 8 respectively, and two layer height 15cm can place a cultivation box respectively on bottom 7 and top layer 8, and the back is put into to the cultivation box, and bottom 7 and top layer 8 observation result are not sheltered from each other, can cultivate 2 sample mould counts simultaneously, effectively utilizes incubator space.
The culture cassette comprises a top cover 1 and a bottom box 2. The bottom box 2 is a rectangular box, and a partition plate 3 which is arranged along the middle line of the length direction of the bottom box and divides the bottom box 2 into two spaces is arranged in the box. Four culture dishes are placed in each space, so that the growth condition of 2-3 plate colonies adapting to dilution and blank control can be directly observed for a single sample at a time. After the partition plate 3 is arranged, eight corners are arranged in the bottom box 2, and round corners 6 are respectively arranged in the eight corners, so that the bottom box round corners are convenient to clean and disinfect, and dirt collection is avoided.
The top cover 1 includes a top panel 11 and side panels 12 provided on both sides of the top panel 11. Set up bacterial colony observation window 4 on the top panel 11, bacterial colony observation window 4 is relative with the culture dish one by one of placing in the end box 2, and culture dish diameter 90mm, bacterial colony observation window 4 diameter 80mm can not shelter from the culture dish when fixed culture dish, but the bacterial colony growth condition on the direct observation culture dish. The center of the top panel 11 is provided with the air holes 5, the air holes 5 are round holes with the diameter of 1cm, mold is aerobic microorganism, the air holes 5 are arranged, the top cover 1 and the bottom box 2 are covered, and the sufficient oxygen in the culture box is ensured while the interference of mold spores is avoided.
The top panel 11 is slightly wider than the bottom box 2, after the top cover 1 and the bottom box 2 are covered, the side plate 12 is covered on the outer side of the bottom box 2, and compared with the structure that the side plate 12 is covered on the inner side of the bottom box 2, the contact opportunity of the external environment and a culture dish in the bottom box 2 can be reduced, and the pollution probability is reduced. In addition, the cover structure of the top cover 1 and the bottom box 2 can meet the cover requirements of the culture dishes with different heights, the top panel 11 contacts with the dish covers of the culture dishes after the cover is covered, and the cover heights of the top cover 1 and the bottom box 2 are different. Only two side plates 12 are arranged, so that the cover of the top cover 1 is more labor-saving when the cover of the bottom box 2 is closed. The end angle of the side plate 12 is set to be an arc angle, so that the probability of scratching the user by the end angle can be reduced and the use safety can be improved relative to setting the end angle to be a right angle.
The culture box and the interlayer frame are made of corrosion-resistant materials, such as transparent PE, PC high-density polyethylene materials and the like, and the materials are high in hardness, resistant to corrosion by ozone, 75% ethanol, hypochlorite, benzalkonium bromide and other disinfectants, and are convenient to clean and disinfect frequently. The transparent material is preferentially corrosion-resistant, and shielding can not be generated during observation.
Finally, it is noted that the above-mentioned preferred embodiments illustrate rather than limit the utility model, and that, although the utility model has been described in detail with reference to the above-mentioned preferred embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the scope of the utility model as defined by the appended claims.
Claims (9)
1. A device for preventing mildew spores from diffusing in mould culture, which is characterized by comprising a culture box; the culture box comprises a top cover (1) and a bottom box (2); a partition plate (3) for separating and placing the culture dishes is arranged in the bottom box (2); the top cover (1) is covered on the bottom box (2), the top cover (1) is provided with colony observation windows (4), the colony observation windows (4) are opposite to the culture dishes placed in the bottom box (2) one by one, and the middle part of the top cover (1) is provided with ventilation holes (5).
2. The device for preventing mildew spores from diffusing in mould culture according to claim 1, wherein the top cover (1) comprises a top panel (11) and side plates (12) arranged on two sides of the top panel (11), and the side plates (12) are covered on the outer side of the bottom box (2).
3. The device for preventing mildew spores from diffusing in mould culture according to claim 2, wherein the end angle of the side plate (12) is a circular arc angle.
4. A device for the diffusion of mould spores in mould culture according to any one of claims 1-3, further comprising a spacer; the interlayer frame is of a multi-layer structure, and the layer height is larger than the height of the culture box.
5. The device for preventing mildew spores from diffusing in mould culture according to claim 4, wherein the bottom box (2) is a rectangular box; the separation plate (3) is provided with one piece, and the separation plate (3) separates the bottom box (2) into two spaces along the central line of the length direction of the bottom box (2).
6. The device for preventing mildew spores from diffusing in mould culture according to claim 5, wherein the eight corners in the bottom box (2) are respectively provided with rounded corners (6).
7. The apparatus for preventing mildew spores from diffusing in mold culture according to claim 5, wherein four culture dishes are placed in each space.
8. The device for preventing mildew spores from diffusing in mold culture according to claim 4, wherein the culture box and the interlayer frame are made of corrosion-resistant materials.
9. The device for preventing mildew spores from diffusing in mould culture according to claim 4, wherein the interlayer frame is provided with two layers, namely a bottom layer (7) and a top layer (8), and a culture box is respectively arranged on the bottom layer (7) and the top layer (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322571817.1U CN220846136U (en) | 2023-09-21 | 2023-09-21 | Device for preventing mildew spores from diffusing in mould culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322571817.1U CN220846136U (en) | 2023-09-21 | 2023-09-21 | Device for preventing mildew spores from diffusing in mould culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220846136U true CN220846136U (en) | 2024-04-26 |
Family
ID=90778602
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322571817.1U Active CN220846136U (en) | 2023-09-21 | 2023-09-21 | Device for preventing mildew spores from diffusing in mould culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220846136U (en) |
-
2023
- 2023-09-21 CN CN202322571817.1U patent/CN220846136U/en active Active
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant |