CN214881529U - Endophytic fungi antagonistic volatile substance screening device - Google Patents
Endophytic fungi antagonistic volatile substance screening device Download PDFInfo
- Publication number
- CN214881529U CN214881529U CN202121484551.1U CN202121484551U CN214881529U CN 214881529 U CN214881529 U CN 214881529U CN 202121484551 U CN202121484551 U CN 202121484551U CN 214881529 U CN214881529 U CN 214881529U
- Authority
- CN
- China
- Prior art keywords
- culture dish
- bacteria culture
- fungi
- volatile substance
- water bath
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of microorganism research and development, in particular to a screening device for antagonistic endophytic fungi volatile substances, which comprises a water bath kettle, wherein the upper part of the water bath kettle is provided with a screening component; screening subassembly includes the fungi culture dish, the fungi culture dish with the contact of water-bath kettle upper portion is connected, the contact of fungi culture dish bottom surface inner wall is provided with the bracket, the contact of bracket top is provided with at the bottom of the bacteria culture dish, the inside fixedly connected with cardboard at the bottom of the bacteria culture dish, the semi-circular boss of top limit portion fixedly connected with at the bottom of the bacteria culture dish, the boss top can be dismantled and be connected with bacteria culture dish lid, bacteria culture dish lid with boss looks adaptation, bacteria culture dish lid upper portion is equipped with the shield. The utility model discloses an inoculate fungi and target bacterial colony respectively in different culture dishes, make endophytic fungi volatile substance direct action target bacterial colony, get rid of the influence that nutrition competition inhibited to play the screening effect.
Description
Technical Field
The utility model relates to a microorganism research and development technical field, concretely relates to endogenetic fungi of antagonism volatile substance sieving mechanism.
Background
Endophytes (endophytes) are normal microorganisms that grow within healthy plant tissues, organs, without causing significant symptoms, and can be isolated from within plants that have been surface sterilized. The plant endophyte is a very rich group, and the antibacterial endophyte as one of the groups can generate a plurality of new antibacterial active substances, can be used for research and development of new medicines in medical treatment and prevention and control of agricultural plant diseases, but the research on the plant antibacterial endophyte is just started at the present stage, and the characteristics of the plant antibacterial endophyte are not deeply known. When the endophytic fungi performs antagonism screening on the target flora, experimenters usually adopt a confronting culture screening method to inoculate the endophytic fungi and the target flora in the same culture dish simultaneously, and the growth condition of the target colony is observed through later-stage culture, so that whether the endophytic fungi perform antagonism on the target colony is judged, and the purpose of screening is achieved. Since the amount of growth of endophytic fungi cannot be effectively controlled in the confronting culture screening method, it is impossible to determine whether the growth is a nutrient competition inhibition or an antagonistic action of volatile substances produced by the fungi, and therefore there is a need for an apparatus that exerts a screening effect by antagonistic action of volatile substances of endophytic fungi on a target colony without being affected by the amount of growth of endophytic fungi.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing an antagonistic endophytic fungi volatile substance sieving mechanism to solve above-mentioned problem, through vaccinating fungi and target bacterial colony respectively in different culture dishes, make endophytic fungi volatile substance direct action target bacterial colony, get rid of the influence that nutrition competition inhibited, play the effect of screening.
In order to achieve the above object, the utility model provides a following scheme:
an endophytic fungi antagonistic volatile substance screening device comprises a water bath, wherein a screening component is arranged at the upper part of the water bath;
screening subassembly includes the fungi culture dish, the fungi culture dish with the contact of water-bath kettle upper portion is connected, the contact of fungi culture dish bottom surface inner wall is provided with the bracket, the contact of bracket top is provided with at the bottom of the bacteria culture dish, the inside fixedly connected with cardboard at the bottom of the bacteria culture dish, the cardboard will separate for two parts at the bottom of the bacteria culture dish, the semi-circular boss of top limit portion fixedly connected with at the bottom of the bacteria culture dish, the boss top can be dismantled and be connected with bacteria culture dish lid, bacteria culture dish lid is semi-circular structure, bacteria culture dish lid with boss looks adaptation, bacteria culture dish lid upper portion is equipped with the shield, shield lower limb portion with water-bath kettle upper edge portion contact connection.
Preferably, the inner side wall of the fungus culture dish is of an inverted cone structure.
Preferably, the inner wall of the bottom surface of the fungus culture dish is provided with a tray in a contact manner, and a plurality of through holes are formed in the tray.
Preferably, the bracket comprises a first bent pipe and a second bent pipe, the first bent pipe and the second bent pipe are both in Contraband-shaped structures, the middle parts of the first bent pipe and the second bent pipe are fixedly connected, and the planes of the first bent pipe and the second bent pipe are perpendicular to each other.
Preferably, a first sealing groove is formed in the bottom of the bacteria culture dish and the top surface of the clamping plate, a second sealing groove is formed in the end face, close to the bottom of the bacteria culture dish, of the bacteria culture dish cover, and a sealing strip is arranged between the first sealing groove and the second sealing groove.
Preferably, the outer wall of the water bath is fixedly connected with a controller, and the bottom surface of the water bath is fixedly connected with a plurality of feet.
Preferably, the fungus culture dish at the bottom of the bacteria culture dish and the bacteria culture dish lid are glass materials, and the dust cover is transparent acrylic materials.
Preferably, the circular arc-shaped edge part of the bacteria culture dish cover is rotatably connected with a plurality of buckles, and the buckles are matched with the bottom surface of the boss.
The utility model discloses has following technological effect: the water bath kettle of the utility model is used for heating the fungus culture dish to accelerate the volatilization of volatile substances; the bracket is used for placing the bottom of the bacteria culture dish, the clamping plate divides the bottom of the bacteria culture dish into two parts of spaces, and the bacteria culture dish cover completely seals one part of space at the bottom of the bacteria culture dish to prevent volatile substances from entering, so that the volatile substances are only in contact with the part of space without the bacteria culture dish cover; the dustproof cover covers the fungus culture dish, the bracket, the bottom of the bacteria culture dish and the bacteria culture dish cover to play a role in isolating the external environment; the utility model discloses an it inoculates respectively in different culture dishes to await measuring antagonistic fungus and target bacterial colony, makes endophytic fungi volatile substance direct action target bacterial colony, gets rid of the influence that nutrition competition inhibited to play the effect of screening.
The fungus culture dish is of an inverted cone structure, so that volatile substances of endophytic fungi can be volatilized upwards, the volatile substances can be contacted with a target bacterial colony, and meanwhile, the tray can be placed conveniently; the tray is used for placing the bottom of the bacteria culture dish, and the through holes in the tray are used for the volatile substances to pass through; the bracket consists of a first bent pipe and a second bent pipe, is used for placing the bottom of the bacteria culture dish, and has smaller barrier effect on the upward volatilization of volatile substances; the sealing strip between the bottom of the bacteria culture dish and the cover of the bacteria culture dish plays a role in sealing, so that a part of space at the bottom of the bacteria culture dish is in a sealed state, and volatile substances are prevented from entering; the water bath is used for heating the fungus culture dish, so that the volatilization of volatile substances can be accelerated, the controller is used for adjusting the temperature of the water bath, and the feet are used for balancing the water bath; the fungus culture dish, the bottom of the bacteria culture dish and the cover of the bacteria culture dish are made of glass materials, so that observation is facilitated, and the dustproof cover is made of transparent acrylic materials, so that observation is facilitated and the injury to operators due to the fact that the dustproof cover is broken after falling is prevented compared with the dustproof cover made of glass materials; the buckle is arranged to be beneficial to the bacteria culture dish cover to compress the bottom of the bacteria culture dish, so that the sealing performance is enhanced.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without inventive labor.
Fig. 1 is a schematic diagram of the explosion structure of the present invention;
FIG. 2 is a bottom structure view of the bacteria culture dish;
FIG. 3 is a schematic view of the structure of a lid of the bacteria culture dish;
fig. 4 is a schematic structural view of embodiment 2 of the present invention.
Wherein, 1, a water bath kettle; 2. a fungus culture dish; 3. a bracket; 4. the bottom of the bacteria culture dish; 5. a bacteria culture dish cover; 6. a dust cover; 7. a tray; 401. clamping a plate; 402. a first seal groove; 403. a boss; 501. a second seal groove; 502. and (5) buckling.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention is described in detail with reference to the accompanying drawings and the detailed description.
Example 1
Referring to fig. 1-3, the screening device for antagonistic endophytic fungi volatile substances comprises a water bath 1, wherein a screening component is arranged at the upper part of the water bath 1;
screening subassembly includes fungi culture dish 2, fungi culture dish 2 is connected with 1 upper portion contact of water-bath, 2 bottom surface inner wall contacts of fungi culture dish are provided with bracket 3, the contact of 3 tops of bracket is provided with at the bottom of the bacteria culture dish 4, the inside fixedly connected with cardboard 401 at the bottom of the bacteria culture dish 4, cardboard 401 separates for two parts at the bottom of the bacteria culture dish 4, 4 semi-circular bosss of limit fixedly connected with 403 at the bottom of the bacteria culture dish, can dismantle above boss 403 and be connected with bacteria culture dish lid 5, bacteria culture dish lid 5 is semi-circular structure, bacteria culture dish lid 5 and boss 403 looks adaptation, 5 upper portions of bacteria culture dish lid are equipped with shield 6, 6 lower limit portions of shield are connected with 1 upper limit portion contact of water-bath. The water bath 1 is used for heating the fungus culture dish 2 to accelerate the volatilization of volatile substances; the bracket 3 is used for placing a bacteria culture dish bottom 4, the clamping plate 401 divides the bacteria culture dish bottom 4 into two parts of space, the bacteria culture dish cover 5 completely seals one part of space of the bacteria culture dish bottom 4 to prevent volatile substances from entering, so that the volatile substances are only contacted with the part of space without the bacteria culture dish cover 5; the fungus culture dish 2, the bracket 3, the bacteria culture dish bottom 4 and the bacteria culture dish cover 5 are covered by the dustproof cover 6 to isolate the external environment; the utility model discloses an inoculate fungi and target bacterial colony respectively in different culture dishes, make endophytic fungi volatile substance direct action target bacterial colony, get rid of the influence that nutrition competition inhibited to play the effect of screening.
Further optimize the scheme, the 2 inside walls of fungi culture dish are the back taper structure. Fungus culture dish 2 of back taper structure is favorable to endogenetic fungi's volatile substance to volatilize upwards, helps the contact of volatile substance and target bacterial colony, the placing of tray 7 of being convenient for.
Further optimize the scheme, the inner wall contact of 2 bottom surfaces of fungi culture dish is provided with tray 7, and tray 7 is inside to be seted up a plurality of through-holes. Tray 7 is used for placing bacteria culture dish bottom 4, and the through-hole on tray 7 is used for the passing through of volatile substance.
According to a further optimized scheme, the bracket 3 comprises a first bent pipe and a second bent pipe, the first bent pipe and the second bent pipe are both of Contraband-shaped structures, the middle parts of the first bent pipe and the second bent pipe are fixedly connected, and the planes of the first bent pipe and the second bent pipe are perpendicular to each other. Bracket 3 comprises first return bend and second return bend for place bacterium culture dish bottom 4, it is littleer to the volatile material effect of hindering that upwards volatilizees.
Further optimization scheme, first seal groove 402 has been seted up to 4 and cardboard 401 top surfaces at the bottom of the bacteria culture dish, and second seal groove 501 has been seted up to the terminal surface that bacteria culture dish lid 5 is close to 4 at the bottom of the bacteria culture dish, is equipped with the sealing strip between first seal groove 402 and the second seal groove 501. The sealing strip plays a sealing role, so that a part of space at the bottom 4 of the bacteria culture dish is in a sealing state, and volatile substances are prevented from entering.
Further optimizing the scheme, the outer wall of the water bath kettle 1 is fixedly connected with a controller, and the bottom surface of the water bath kettle 1 is fixedly connected with a plurality of feet. The water bath 1 is used for heating the fungus culture dish 2, is favorable to the volatile matter to volatilize with higher speed, and the controller is used for adjusting the temperature of water bath, and the lower margin helps the balance of water bath.
Further optimize the scheme, fungi culture dish 2, bacteria culture dish bottom 4 and bacteria culture dish lid 5 are the glass material, and shield 6 is transparent ya keli material. Fungi culture dish 2, bacteria culture dish bottom 4 with bacteria culture dish lid 5 adopts the glass material, the observation of being convenient for, shield 6 is transparent ya keli material, compares in the shield 6 of glass material, and the observation of both being convenient for prevents again that shield 6 from dropping the back cracked and cause the injury to operating personnel.
Further optimizing scheme, the circular arc limit portion of bacteria culture dish lid 5 rotates and is connected with a plurality of buckles 502, and buckle 502 cooperatees with boss 403 bottom surface. The buckle 502 is arranged to facilitate the bacteria culture dish cover 5 to compress the bacteria culture dish bottom 4, so that the sealing performance is enhanced.
The working process of the embodiment is as follows: firstly, inoculating endophytic fungi for research into a fungus culture dish 2 for culture, and after hyphae grow out of the fungi, preventing the fungus culture dish 2 from being placed in a water bath 1; simultaneously inoculating target bacterial colonies to two sides of an inner clamping plate 401 of a bacteria culture dish bottom 4, covering a bacteria culture dish cover 5 to enable the bacterial colonies on one side of the inner clamping plate 401 to be exposed in the air, enabling the bacterial colonies on the other side to be in a sealed state, placing a bracket 3 on the bacteria culture dish bottom 4, and placing the bacteria culture dish bottom 4 and the bacteria culture dish cover 5 above the bracket 3; then the dustproof cover 6 is covered above the water bath 1, and the temperature of the water bath is adjusted according to the requirement. If the growth of the target colony exposed on one side of the volatile substances is inhibited, and the target colony in a sealed state normally grows, the fungal volatile substances have antagonistic action on the target colony; if the growth state of the target colony exposed on the volatile substance side is the same as that of the target colony in the sealed state, the fungal volatile substance has no antagonistic effect on the target colony.
Example 2
Referring to fig. 4, the only difference between this embodiment and embodiment 1 is that the tray 7 is used to place the bottom 4 of the bacteria culture dish, so that the bottom 4 of the bacteria culture dish is placed more stably, and this embodiment is suitable for endophytic fungi with strong volatility because the tray 7 has stronger blocking effect on volatile substances than the tray 3.
In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are merely for convenience of description of the present invention, and do not indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
The above-mentioned embodiments are only intended to describe the preferred embodiments of the present invention, but not to limit the scope of the present invention, and those skilled in the art should also be able to make various modifications and improvements to the technical solution of the present invention without departing from the spirit of the present invention, and all such modifications and improvements are intended to fall within the scope of the present invention as defined in the appended claims.
Claims (8)
1. Antagonistic endophytic fungi volatile substance screening device, its characterized in that: the device comprises a water bath pot (1), wherein a screening component is arranged at the upper part of the water bath pot (1);
the screening component comprises a fungus culture dish (2), the fungus culture dish (2) is in contact connection with the upper portion of the water bath pot (1), a bracket (3) is arranged on the inner wall of the bottom surface of the fungus culture dish (2) in a contact manner, a bacterium culture dish bottom (4) is arranged above the bracket (3) in a contact manner, a clamping plate (401) is fixedly connected inside the bacterium culture dish bottom (4), the bacterium culture dish bottom (4) is divided into two parts by the clamping plate (401), a semicircular boss (403) is fixedly connected to the upper side portion of the bacterium culture dish bottom (4), a bacterium culture dish cover (5) is detachably connected to the upper side portion of the boss (403), the bacterium culture dish cover (5) is of a semicircular structure, the bacterium culture dish cover (5) is matched with the boss (403), and a dustproof cover (6) is arranged on the upper portion of the bacterium culture dish cover (5), the lower edge part of the dustproof cover (6) is in contact connection with the upper edge part of the water bath kettle (1).
2. An antagonistic endophytic fungi volatile substance screening device according to claim 1, characterized in that: the inner side wall of the fungus culture dish (2) is of an inverted cone structure.
3. An antagonistic endophytic fungi volatile substance screening device according to claim 1, characterized in that: the fungus culture dish (2) bottom surface inner wall contact is provided with tray (7), a plurality of through-holes have been seted up to tray (7) inside.
4. An antagonistic endophytic fungi volatile substance screening device according to claim 1, characterized in that: the bracket (3) comprises a first bent pipe and a second bent pipe, the first bent pipe and the second bent pipe are both of Contraband-shaped structures, the middle parts of the first bent pipe and the second bent pipe are fixedly connected, and the planes of the first bent pipe and the second bent pipe are perpendicular to each other.
5. An antagonistic endophytic fungi volatile substance screening device according to claim 1, characterized in that: first seal groove (402) have been seted up at bacterium culture dish bottom (4) with cardboard (401) top surface, bacterium culture dish lid (5) are close to second seal groove (501) have been seted up to the terminal surface at the bottom of the bacterium culture dish (4), first seal groove (402) with be equipped with the sealing strip between second seal groove (501).
6. An antagonistic endophytic fungi volatile substance screening device according to claim 1, characterized in that: the outer wall of the water bath pot (1) is fixedly connected with a controller, and the bottom surface of the water bath pot (1) is fixedly connected with a plurality of feet.
7. An antagonistic endophytic fungi volatile substance screening device according to claim 1, characterized in that: fungus culture dish (2) bacterial culture dish bottom (4) with bacterial culture dish lid (5) are the glass material, shield (6) are transparent ya keli material.
8. An antagonistic endophytic fungi volatile substance screening device according to claim 1, characterized in that: the circular arc-shaped edge part of the bacteria culture dish cover (5) is rotatably connected with a plurality of buckles (502), and the buckles (502) are matched with the bottom surface of the boss (403).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121484551.1U CN214881529U (en) | 2021-07-01 | 2021-07-01 | Endophytic fungi antagonistic volatile substance screening device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121484551.1U CN214881529U (en) | 2021-07-01 | 2021-07-01 | Endophytic fungi antagonistic volatile substance screening device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214881529U true CN214881529U (en) | 2021-11-26 |
Family
ID=78907525
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121484551.1U Active CN214881529U (en) | 2021-07-01 | 2021-07-01 | Endophytic fungi antagonistic volatile substance screening device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214881529U (en) |
-
2021
- 2021-07-01 CN CN202121484551.1U patent/CN214881529U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Fokkema et al. | Aphid honeydew, a potential stimulant of Cochliobolus sativus and Septoria nodorum and the competitive role of saprophytic mycoflora | |
| CN104823714B (en) | A kind of method for massively culturing of northern Chinese caterpillar Fungus | |
| CN103609329B (en) | Cordyceps militaris culturing method capable of improving cordycepin content | |
| CN105816897B (en) | A kind of organic waste deodorant and preparation method thereof containing microbial strains | |
| CN109355233A (en) | A kind of bacillus amyloliquefaciens and its application | |
| CN105219655A (en) | Flavus biocontrol strain and the application thereof of aflatoxin do not produced to sterilant resistance | |
| CN106119131A (en) | A kind of method alleviating excess copper suppression Growth of Pleurotus Mycelium | |
| CN1065710C (en) | High quality and high yield agaricus tabularis cultivation seed culture new technology | |
| CN104911111B (en) | A kind of Metarhizium anisopliae solid culture method | |
| CN214881529U (en) | Endophytic fungi antagonistic volatile substance screening device | |
| CN209338566U (en) | A kind of biotechnology cell culture apparatus | |
| CN1236052C (en) | Bacillus subtilis strain | |
| CN102286385B (en) | Method for carrying out solid culturing on beauveria bassiana by stages | |
| CN108660088A (en) | Endophytic Bacteria in Cotton YUPP-10 and its application in cotton verticillium wilt prevention | |
| CN108893423A (en) | A kind of microbial product and its manufacturing method for administering heavy metal pollution salt-soda soil | |
| US8871502B2 (en) | Culture medium device | |
| KR101679243B1 (en) | Mushroom cultivating kit, and method for cultivating mushroom using thereof | |
| CN206078185U (en) | Device is cultivated to domestic fungus cake | |
| CN114149935B (en) | Lysobacter enzymogenes and application thereof | |
| CN105519353A (en) | Agaricus bisporus spawn preparation method | |
| Magae | Saponin stimulates fruiting of the edible basidiomycete Pleurotus ostreatus | |
| KR20200061556A (en) | A Method for Cultivating a White Shiitake Mushroom Containing a Selenium | |
| CN109392614A (en) | A kind of two-sided culture apparatus of entomogenous fungi | |
| Islam et al. | The management of humidifying treatment for low contamination risks during indoor cultivation of grey oyster mushroom (Pleurotus pulmonarius) | |
| CN106171528A (en) | The implantation methods of Black Ganoderma high yield and planting unit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |