CN109392614A - A kind of two-sided culture apparatus of entomogenous fungi - Google Patents
A kind of two-sided culture apparatus of entomogenous fungi Download PDFInfo
- Publication number
- CN109392614A CN109392614A CN201811008390.1A CN201811008390A CN109392614A CN 109392614 A CN109392614 A CN 109392614A CN 201811008390 A CN201811008390 A CN 201811008390A CN 109392614 A CN109392614 A CN 109392614A
- Authority
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- China
- Prior art keywords
- box
- culture
- magnet
- partition
- entomogenous fungi
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000233866 Fungi Species 0.000 title claims abstract description 23
- 238000005192 partition Methods 0.000 claims abstract description 29
- 239000000523 sample Substances 0.000 claims abstract description 8
- 239000000463 material Substances 0.000 claims abstract description 3
- 239000000696 magnetic material Substances 0.000 claims description 3
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 3
- 238000001179 sorption measurement Methods 0.000 claims description 2
- 241001248610 Ophiocordyceps sinensis Species 0.000 description 7
- 241001264174 Cordyceps militaris Species 0.000 description 4
- 238000005286 illumination Methods 0.000 description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000012943 hotmelt Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- OILXMJHPFNGGTO-UHFFFAOYSA-N (22E)-(24xi)-24-methylcholesta-5,22-dien-3beta-ol Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(C)C=CC(C)C(C)C)C1(C)CC2 OILXMJHPFNGGTO-UHFFFAOYSA-N 0.000 description 1
- RQOCXCFLRBRBCS-UHFFFAOYSA-N (22E)-cholesta-5,7,22-trien-3beta-ol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CCC(C)C)CCC33)C)C3=CC=C21 RQOCXCFLRBRBCS-UHFFFAOYSA-N 0.000 description 1
- OQMZNAMGEHIHNN-UHFFFAOYSA-N 7-Dehydrostigmasterol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CC(CC)C(C)C)CCC33)C)C3=CC=C21 OQMZNAMGEHIHNN-UHFFFAOYSA-N 0.000 description 1
- 241000931705 Cicada Species 0.000 description 1
- 241001480006 Clavicipitaceae Species 0.000 description 1
- 241000190633 Cordyceps Species 0.000 description 1
- 241000422920 Cordyceps gunnii Species 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- DNVPQKQSNYMLRS-NXVQYWJNSA-N Ergosterol Natural products CC(C)[C@@H](C)C=C[C@H](C)[C@H]1CC[C@H]2C3=CC=C4C[C@@H](O)CC[C@]4(C)[C@@H]3CC[C@]12C DNVPQKQSNYMLRS-NXVQYWJNSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 240000006698 Spigelia anthelmia Species 0.000 description 1
- 229930003779 Vitamin B12 Natural products 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- DNVPQKQSNYMLRS-SOWFXMKYSA-N ergosterol Chemical compound C1[C@@H](O)CC[C@]2(C)[C@H](CC[C@]3([C@H]([C@H](C)/C=C/[C@@H](C)C(C)C)CC[C@H]33)C)C3=CC=C21 DNVPQKQSNYMLRS-SOWFXMKYSA-N 0.000 description 1
- 238000004362 fungal culture Methods 0.000 description 1
- 150000002402 hexoses Chemical class 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 238000009655 industrial fermentation Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 239000011715 vitamin B12 Substances 0.000 description 1
- 235000019163 vitamin B12 Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/64—Cultivation containers; Lids therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/69—Arrangements for managing the environment, e.g. sprinklers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to a kind of two-sided culture apparatus of entomogenous fungi, including cultivating box, lid of the culture box by box body and for enclosed box body opening is formed, and culture box is molded by the material PVC or PP of food-grade transparent, it cultivates box and is equipped with several air holes, sterile film is covered at air hole;It cultivates and is equipped with partition in box, the partition opposite can cultivate box and move up and down adjusting height;Culture cassette bottom portion and top are separately installed with changeable colour LED light source, and the changeable colour LED light source is connect with dimming device;It cultivates and is equipped with temp probe and moisture probe in box.The present invention is implemented for double cultures of entomogenous fungi to increase the yield of single culture.
Description
Technical field
The present invention relates to fungal culture field more particularly to a kind of two-sided culture apparatus of entomogenous fungi.
Background technique
Entomogenous fungi culture be at most Clavicipitaceae fungi a variety of lepidopterous insects pupas and larva stroma and
The complex of larva corpse growth, this kind of entomogenous fungi is referred to as cordyceps sinensis, has had now been found that more than 500 kinds, such as cordyceps sinensis, cicada fungus
Cordyceps sinensis, Cordyceps hawkesii Gary, liangshan cordyceps herb, Xinjiang cordyceps sinensis, branch cordyceps sinensis, the leaves of pulse plants gram cordyceps sinensis, Cordyceps militaris etc..Natural cs contain various
The bioactive substance of various kinds cordycepic acid rich in, unsaturated fatty acid, vitamin B12, ergosterol, hexose alcohol,
Alkaloid etc..It factually tests studies have shown that natural cs, which have, adjusts function of immune system, antitumor action, antifatigue, disease-resistant
Poison adjusts a variety of medicinal efficacies such as central nervous system function.Wild worm grass resources are limited, due to excessively excavating, cordyceps sinensis
The ecological environment of growth is seriously destroyed, thus artificial cultivation seasonable and send out, in recent years, utilize industrial fermentation technique of fungi
To be it is extensive, by Cordyceps Militaris carry out liquid or solid fermented and cultured, market shows that its tunning has very on food and medicine
Good application value, such as Cordyceps militaris, Periostracum cicadae, Cordyceps militaris, Periostracum cicadae etc. currently on the market is all artificial single-sided training
Feeding, incubation time is substantially at 20-40 days.Realize that two-sided culture can effectively increase the yield of coremium, it is therefore desirable to provide one
Kind can be for realizing the culture apparatus of two-sided culture.
Summary of the invention
To solve the above-mentioned problems, the purpose of the present invention is to provide a kind of two-sided culture apparatus of entomogenous fungi, Neng Gouyong
Increase the yield of single culture in double cultures of realization entomogenous fungi.
In order to achieve the above purpose, present invention employs technical solutions below:
A kind of two-sided culture apparatus of entomogenous fungi, including culture box, culture box is by box body and for enclosed box body opening
Lid composition, culture box are molded by the material PVC or PP of food-grade transparent, and culture box is equipped with several air holes, saturating
Sterile film is covered at stomata;It cultivates and is equipped with partition in box, the partition opposite can cultivate box and move up and down adjusting height;Cultivate box
Bottom and top are separately installed with changeable colour LED light source, and the changeable colour LED light source is connect with dimming device;It is set in culture box
There are temp probe and moisture probe.
Preferably, partition is at least equipped with magnet A in symmetrical two side ends, it is equipped with adjusting bracket outside culture box, is adjusted
Bracket is equipped with the magnet A setting corresponded on partition, for the magnet B of adsorption magnet A, and adjusting bracket includes pedestal and stands
Frame, magnet B, which can move up and down, to be arranged in stand.Cooperated by the magnet on external magnet and culture box internal partition, is adjusted
The height of external magnet realizes the adjusting of divider height, do not need setting partition and cultivates the connection between box, and structure is simple,
The opposing seal environment in culture box will not be destroyed.
As an implementation, stand completely or partially uses magnetic material to support, and magnet B is adsorbed in stand.Directly
It is simple to connect connection height of the regulating magnet B in stand, height adjustment mode by the way of magnetic absorption.
As another embodiment, stand is equipped with sliding slot, and magnet B is arranged in the sliding slot and can be above and below the sliding slot
It slides, multiple height adjusting holes being set side by side is equipped in sliding slot, retainer is disposed therein in an adjustment hole to limit magnetic
The height of iron B.Using the height adjustment path of sliding slot limitation magnet B, realizes and exist to magnet B using retainer and adjustment hole cooperation
The limit of upper-lower height in sliding slot, such as the adjustment hole kind in one of height is connected to by bolt and nut and prevents magnet B
It glides, adjustment structure is simple.
Preferably, the lid is plate structure, at the top of culture box, air hole is arranged on lid for lid setting.
In this way, convenient use hot melt that sterile film is arranged at air hole.
Above-mentioned culture apparatus can provide a kind of gnotobasis of opposing seal by culture box to carry out entomogenous fungi training
It supports, and height-adjustable partition is set in inside, partition upper and lower side is made to be respectively formed culture space;It is mentioned respectively from upper and lower side simultaneously
For being irradiated to the light source of partition, so as to realize two-sided culture.
Detailed description of the invention
Fig. 1 is the structural schematic diagram that box is cultivated in the present invention.
Specific embodiment
The preferred embodiment of this patent is described in further detail with reference to the accompanying drawing.
The two-sided culture apparatus of a kind of entomogenous fungi as shown in Figure 1, including culture box 1 and adjusting bracket 2, culture box 1 by
Box body and the lid composition being open for enclosed box body, culture box are equipped with several air holes 11, are covered at air hole sterile
Film, sterile membrane aperture are 0.22-0.45 μm.In this way, a sterile culture environment can be formed by making to cultivate inside box 1, and
Air needed for culture can be provided.Being preferably provided with lid is plate structure, and at the top of culture box, air hole setting exists for lid setting
On lid.In this way, convenient use hot melt that sterile film is arranged at air hole.
Cultivate and be equipped with partition 12 in box, partition 12 is apertured partition, in fact on be distributed with and grown downwards for entomogenous fungi
Hole, the partition 12 opposite can cultivate box 1 and move up and down adjusting height.In the present embodiment, culture box 1 is long 30-60cm, width
The hexahedron of 30- 50cm, high 25-50cm, the height adjustment range of partition 12 are 0-20cm.Entomogenous fungi is enterprising in partition 12
Row culture, partition 12 can be respectively formed the growing space of entomogenous fungi in lower part on it in culture box, and cultivate box by food
Grade transparent material PVC or PP are molded, and cultivate cassette bottom portion and top is separately installed with changeable colour LED light source 13 from upper and lower
Entomogenous fungi on respectively partition provides illumination, in order to irradiate uniformly, generally sets LED light for changeable colour LED light source
Band.Changeable colour LED light source 13 can provide three kinds of white light, blue light and feux rouges illumination, and intensity of illumination can be changed between 100-1000LX
Color LED light source 13 is connect with dimming device (dimming device is not illustrated in figure), and dimming device controls the color of variable color light source
Switching and intensity of illumination are adjusted.
In the application, in order to realize the height adjustment to partition, partition 12 is at least equipped with magnet in opposite two side ends
A121, culture box outside are equipped with adjusting bracket 2, and adjusting bracket 2 is equipped with the setting of magnet A 121 corresponded on partition, for inhaling
The magnet 221B of attached magnet A 121, adjusting bracket 2 include pedestal 21 and stand 22, and magnet B 221, which can move up and down, to be arranged in stand
On 22.As one of embodiment, stand 22 is all or part of to be supported using magnetic material, and magnet B 221 is adsorbed on vertical
On frame.Connection height of the mode regulating magnet B of magnetic absorption in stand is directlyed adopt, height adjustment mode is simple.As
Another embodiment, stand 22 are equipped with sliding slot, and magnet B 221 is arranged in the sliding slot and can slide up and down along the sliding slot, sliding
Multiple height adjusting holes being set side by side are equipped in slot, retainer is disposed therein the height that magnet B is limited in an adjustment hole
Degree.Using the height adjustment path of sliding slot limitation magnet B, realized to magnet B in sliding slot using retainer and adjustment hole cooperation
The limit of upper-lower height, such as the adjustment hole kind in one of height is connected to by bolt and nut, magnet B is prevented to glide, it adjusts
Section structure is simple.
Due to needing the temperature and humidity of strict control culture environment in incubation, temperature is equipped in culture box
Degree probe 14 and moisture probe 15, the monitoring so as to operator to culture environment.
Above-mentioned culture apparatus can provide a kind of gnotobasis of opposing seal by culture box to carry out entomogenous fungi training
It supports, and height-adjustable partition is set in inside, partition upper and lower side is made to be respectively formed culture space;It is mentioned respectively from upper and lower side simultaneously
For being irradiated to the light source of partition, so as to realize two-sided culture.
Claims (5)
1. a kind of two-sided culture apparatus of entomogenous fungi, including culture box, culture box is by box body and for the lid of enclosed box body opening
Body composition, which is characterized in that culture box is molded by the material PVC or PP of food-grade transparent, is parched if culture box is equipped with
Stomata is covered with sterile film at air hole;It is interior equipped with partition to cultivate box, the partition opposite can cultivate box and move up and down adjusting height
Degree;Culture cassette bottom portion and top are separately installed with changeable colour LED light source, and the changeable colour LED light source is connect with dimming device;Training
It supports and is equipped with temp probe and moisture probe in box.
2. the two-sided culture apparatus of a kind of entomogenous fungi according to claim 1, which is characterized in that partition is at least opposite
Two side ends be equipped with magnet A, culture box outside be equipped with adjusting bracket, adjusting bracket be equipped with correspond to partition on magnet A setting,
For the magnet B of adsorption magnet A, adjusting bracket includes pedestal and stand, and magnet B, which can move up and down, to be arranged in stand.
3. the two-sided culture apparatus of a kind of entomogenous fungi according to claim 2, which is characterized in that stand is completely or partially adopted
It is supported with magnetic material, magnet B is adsorbed in stand.
4. the two-sided culture apparatus of a kind of entomogenous fungi according to claim 2, which is characterized in that stand is equipped with sliding slot,
Magnet B is arranged in the sliding slot and can slide up and down along the sliding slot, multiple height adjusting holes being set side by side is equipped in sliding slot, only
Moving part is disposed therein the height that magnet B is limited in an adjustment hole.
5. the two-sided culture apparatus of a kind of entomogenous fungi according to claim 1, which is characterized in that the lid is plate knot
Structure, at the top of culture box, air hole is arranged on lid for lid setting.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201811008390.1A CN109392614A (en) | 2018-08-31 | 2018-08-31 | A kind of two-sided culture apparatus of entomogenous fungi |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN201811008390.1A CN109392614A (en) | 2018-08-31 | 2018-08-31 | A kind of two-sided culture apparatus of entomogenous fungi |
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CN201811008390.1A Pending CN109392614A (en) | 2018-08-31 | 2018-08-31 | A kind of two-sided culture apparatus of entomogenous fungi |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112646718A (en) * | 2020-12-31 | 2021-04-13 | 太仓艺斯高医疗器械科技有限公司 | Inclined inner container structure for carbon dioxide incubator |
CN114568282A (en) * | 2022-03-30 | 2022-06-03 | 扬州大学 | Identification pool for salt tolerance identification of rice seedlings and use method thereof |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20160345504A1 (en) * | 2015-05-29 | 2016-12-01 | Tunghai University | Method for culturing ophiocordyceps sinensis |
CN106318875A (en) * | 2015-06-18 | 2017-01-11 | 浙江泛亚生物医药股份有限公司 | Cordyceps sobolifera two-way artificial culture method |
CN207070692U (en) * | 2017-06-08 | 2018-03-06 | 江西康丰生物科技有限公司 | A kind of edible mushroom cultivating device |
CN207100015U (en) * | 2017-03-16 | 2018-03-16 | 谭树培 | Bailer with gondola water faucet function |
CN208956576U (en) * | 2018-08-31 | 2019-06-11 | 湖州蝉知堂生物科技有限公司 | A kind of two-sided culture apparatus of entomogenous fungi |
-
2018
- 2018-08-31 CN CN201811008390.1A patent/CN109392614A/en active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20160345504A1 (en) * | 2015-05-29 | 2016-12-01 | Tunghai University | Method for culturing ophiocordyceps sinensis |
CN106318875A (en) * | 2015-06-18 | 2017-01-11 | 浙江泛亚生物医药股份有限公司 | Cordyceps sobolifera two-way artificial culture method |
CN207100015U (en) * | 2017-03-16 | 2018-03-16 | 谭树培 | Bailer with gondola water faucet function |
CN207070692U (en) * | 2017-06-08 | 2018-03-06 | 江西康丰生物科技有限公司 | A kind of edible mushroom cultivating device |
CN208956576U (en) * | 2018-08-31 | 2019-06-11 | 湖州蝉知堂生物科技有限公司 | A kind of two-sided culture apparatus of entomogenous fungi |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112646718A (en) * | 2020-12-31 | 2021-04-13 | 太仓艺斯高医疗器械科技有限公司 | Inclined inner container structure for carbon dioxide incubator |
CN114568282A (en) * | 2022-03-30 | 2022-06-03 | 扬州大学 | Identification pool for salt tolerance identification of rice seedlings and use method thereof |
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