CN106039364A - Mink house air sterilization spray and preparation method thereof - Google Patents

Abstract

The invention discloses a mink house air sterilization spray and a preparation method thereof. The mink house air sterilization spray is prepared from, by weight, 12-16 parts of gallnuts, 6-8 parts of spreading hedyotis herb, 4-6 parts of dandelion, 6-9 parts of mangosteen peels, 10-15 parts of fresh purslane, 7-10 parts of radix sophorae flavescentis, 2-5 parts of a humifuse euphorbia herb, 3-5 parts of maidenhair, 2-4 parts of myrrh, 4-6 parts of all-grass of Chinese brake, 15-24 parts of chamomile hydrosol, 6-9 parts of fresh kochia scoparia, 8-10 parts of fresh enteromorpha and 1-3 parts of white mulberry root barks. The mink house air sterilization spray can rapidly and effectively restrain pathogenic bacteria of hemorrhagic pneumonia, is directly sprayed in the air, can play a very good sterilization and disinfection effect, is safe and reliable in composition, does not irritate mink skin, effectively prevents minks from mistakenly eating the residual spray and accordingly prevents the health of the minks from being influenced.

Description

A kind of ermine house air sterillization spray and preparation method thereof

Technical field

The present invention relates to a kind of ermine house air sterillization spray and preparation method thereof.

Background technology

Mink is a kind of precious fur-bearing animal with higher economic worth, and it is to rearing conditions such as feed formula, environment Health etc. all have higher requirements.In especially annual summer, mink is in molt and heat, easily grows thin in ermine house Bacterium, ermine house sterilization is insufficient, easily causes infectious disease, if mink hemorrhagic pneumonia is exactly that one is mainly in summer, and rate till death The highest infectious disease.Mink hemorrhagic pneumonia is mainly by bacillus pyocyaneus, Klebsiella pneumonia and Diplococcus pneumoniae, big Enterobacterias etc. are individually or the mink respiratory infectious disease that causes of various bacteria mixed infection.The morbidity of this disease is anxious, dead fast, often in ground Side's property outbreak of epidemic.Mink all can infect primary disease regardless of kind, age and sex, M & M all 10%～ 50%.In China, bacillus pyocyaneus and Klebsiella pneumonia are the main pathogen causing hemorrhagic pneumonia, have respectively 50% and The case of more than 20% with this both relevant, and both mixed infections common.Make a definite diagnosis this should immediately the whole audience be carried out thoroughly after being ill Sterilization, does environmental health the cage tool polluting the ermine that dies of illness and ground thorough disinfection well, and existing disinfectant solution is to bacillus pyocyaneus and lung The inhibitory action of scorching klebsiella is unsatisfactory, is applied to ermine house, the sterilization of ermine cage can not obtain preferable effect, strengthens sterilization Although pathogen can be killed effect to strengthening it by drug level, but can work the mischief mink health equally.Meanwhile, often Disinfecting drug typically there is toxicity and skin irritation, the disinfecting drug of high concentration contamination mink skin is easily to mink skin Damaging or cause skin allergy, mink eats the disinfecting drug being infected with in cage tool or food dish by mistake, also can make mink health Become harm.

Summary of the invention

It is an object of the invention to provide a kind of ermine house air sterillization spray and preparation method thereof, this disinfecting sprays can be fast Pass the pathogenic bacterium effectively suppressing hemorrhagic pneumonia, be directly sprayed in air and can play good sterilizing effect, and join Side is safe and reliable, will not produce mink skin and stimulate, and effectively prevents mink from eating left drug alignment health by mistake and damages.

To achieve these goals, the technical scheme that the present invention provides is as follows:

A kind of ermine house air sterillization spray, raw material composition and the parts by weight of making described air spray are: Galla Chinensis 12- 16 parts, Herba Hedyotidis Diffusae 6-8 part, Herba Taraxaci 4-6 part, peel of Carcinia mangostana L. 6-9 part, Herba Portulacae fresh goods 10-15 part, Radix Sophorae Flavescentis 7-10 part, Herba Euphorbiae Humifusae 2-5 part, Herba Tagetis Patulae 3-5 part, Myrrha 2-4 part, Herba Pteridis multifidae 4-6 part, Flos Matricariae chamomillae hydrosol 15-24 part, Kochia scoparia fresh goods 6-9 part, Entermorpha fresh goods 8-10 part, Cortex Mori 1-3 part.

Preferably, make described air spray raw material composition and parts by weight be: Galla Chinensis 12 parts, Herba Hedyotidis Diffusae 6 Part, Herba Taraxaci 4 parts, peel of Carcinia mangostana L. 6 parts, Herba Portulacae fresh goods 10 parts, Radix Sophorae Flavescentis 7 parts, Herba Euphorbiae Humifusae 2 parts, Herba Tagetis Patulae 3 parts, Myrrha 2 parts, Herba Pteridis multifidae 4 parts, Flos Matricariae chamomillae hydrosol 15 parts, Kochia scoparia fresh goods 6 parts, Entermorpha fresh goods 8 parts, Cortex Mori 1 part.

Preferably, make described air spray raw material composition and parts by weight be: Galla Chinensis 16 parts, Herba Hedyotidis Diffusae 8 Part, Herba Taraxaci 6 parts, peel of Carcinia mangostana L. 9 parts, Herba Portulacae fresh goods 15 parts, Radix Sophorae Flavescentis 10 parts, Herba Euphorbiae Humifusae 5 parts, Herba Tagetis Patulae 5 parts, Myrrha 4 parts, Herba Pteridis multifidae 6 parts, Flos Matricariae chamomillae hydrosol 24 parts, Kochia scoparia fresh goods 9 parts, Entermorpha fresh goods 10 parts, Cortex Mori 3 parts.

Preferably, make described air spray raw material composition and parts by weight be: Galla Chinensis 14 parts, Herba Hedyotidis Diffusae 7 Part, Herba Taraxaci 5 parts, peel of Carcinia mangostana L. 7 parts, Herba Portulacae fresh goods 13 parts, Radix Sophorae Flavescentis 9 parts, Herba Euphorbiae Humifusae 4 parts, Herba Tagetis Patulae 4 parts, Myrrha 3 parts, Herba Pteridis multifidae 5 parts, Flos Matricariae chamomillae hydrosol 20 parts, Kochia scoparia fresh goods 7 parts, Entermorpha fresh goods 9 parts, Cortex Mori 2 parts.

The invention also discloses the preparation method of described ermine house air sterillization spray, comprise the following steps:

(1) Galla Chinensis, Herba Hedyotidis Diffusae, Herba Taraxaci, Radix Sophorae Flavescentis, Herba Euphorbiae Humifusae, Herba Tagetis Patulae, Myrrha, Herba Pteridis multifidae, Cortex Mori corium farinosum The broken 80 mesh mixed-powders that obtain, the water soaking of gained mixed-powder addition gross mass multiple 5 times 1 hour, slow fire boiling 1 hour, decoct Cross 200 mesh drainage screens after boiling and obtain filtrate, again the water soaking of addition mixed-powder gross mass multiple 5 times 1 hour, slow fire boiling 1 Cross 200 mesh drainage screens after hour and obtain filtrate, merge twice gained filtrate and be cooled to room temperature, standby；

(2) peel of Carcinia mangostana L., Herba Portulacae fresh goods, Kochia scoparia fresh goods, Entermorpha fresh goods are cut into small pieces after cleaning, and add step (1) Gained filtrate grinds pulping after soaking 1 hour, crosses 200 mesh drainage screens and obtain filtrate after standing 3 hours；

(3) step (2) gained filtrate cryoconcentration (less than 40 DEG C) to relative density is the dense of 1.20 ± 0.02 (30 DEG C) Contracting liquid；

(4) after step (3) gained concentrated solution adds Flos Matricariae chamomillae hydrosol mix homogeneously, ultraviolet sterilization 15 minutes, obtains target Thing.

Using method: the air sterillization spray of the present invention is sprayed in the air of ermine house can be played by high-pressure nozzle Sterilizing effect.

Hemorrhagic pneumonia, as the highest infectious disease of a kind of M & M, once finds that sick ermine needs Ermine house is carried out pasteurised completely at once, prevent pathogenic bacterium from infecting healthy mink in ermine house further, cause tremendous economic to lose.This The raw material selected by air sterillization spray of invention has the feature that

Galla Chinensis: cold in nature, sour in the mouth, puckery, there is the effects such as pathogenic fire reducing of astringing the lung, relieving diarrhea with astringents, arresting sweating hemostasis, removing dampness sore, main Controlling the diseases such as chronic cough of deficiency lung, chronic diarrhea chronic dysentery, hemorrhoidal bleeding of having blood in stool, carbuncle sore tumefacting virus, Skin wet and rotten, Galla Chinensis decoct is to Staphylococcus aureus Bacterium, beta hemolytic streptococcus, streptococcus pneumoniae, bacillus pyocyaneus, dysentery bacterium, anthrax bacillus, diphtheria corynebacterium, escherichia coli, wound Cold and Salmonella paratyphi etc. all has the most antibacterial and bactericidal action；

Herba Hedyotidis Diffusae: heat-clearing and toxic substances removing, diuresis of blood circulation promoting, for tonsillitis, pharyngolaryngitis, urinary tract infection, pelvic inflammatory disease, vermiform appendix Inflammation, hepatitis, bacillary dysentery, venom, tumor, also can be used for alimentary tract cancer, and modern pharmacology research proves, Hedyotidis Diffusae can increase The immunity of strong body, the growth of suppression tumor cell, to bacillus pyocyaneus, staphylococcus aureus, streptococcus pneumoniae, dysentery bacterium Inhibitory action is had in pathogenic bacterium；

Herba Taraxaci: micro-hardship, cold, there are the effects such as diuresis, cathartic, jaundice eliminating subcutaneous ulcer, function of gallbladder promoting, control pyretic toxicity, carbuncle, skin infection, abscess of internal organ, mesh Red swell and ache, damp and hot, the puckery pain of jaundice, dribbling urination, furuncle swelling toxin, acute mastitis, scrofula, toothache, conjunctival congestion, pharyngalgia, lung abscess, acute appendicitis, wet Hot jaundice, the puckery pain of pyretic stranguria, control acute mastitis, lymphadenitis, scrofula, furuncle carbuncle toxin, acute conjunctivitis, cold, fever, acute Tonsillitis, acute bronchitis, gastritis, hepatitis, cholecystitis, urinary tract infection etc., Herba Taraxaci is to Diplococcus pneumoniae, meningitis ball Bacterium, diphtheria corynebacterium, bacillus pyocyaneus, dysentery bacterium, Bacillus typhi etc. and micrococcus catarrhalis also have certain bactericidal action；

Peel of Carcinia mangostana L.: slightly cool in nature, diminishing inflammation, removing pain, heat-clearing and toxic substances removing, dampness removing and anti-diarrhea effect, mangosteen skin is rich in tannic acid, single Thujic acid is to clinical common isolated strains such as staphylococcus aureus, staphylococcus epidermidis, staphylococcus haemolyticus, enterococcus faecalis, dung Enterococcus, escherichia coli, Klebsiella Pneumoniae, Pseudomonas aeruginosa, Acinetobacter bauamnnii etc. have antibacterial action and can be with shadow Ringing biomembranous formation, mangosteen skin is rich in procyanidin, and procyanidin is a kind of bioflavonoids having special molecular structure, Being the removing maximally effective Natural antioxidant of people's interior free yl generally acknowledged the most in the world, procyanidin can strengthen blood capillary Pipe, tremulous pulse and vein blood vessel, it has effect that detumescenceization is become silted up, the drag reduction of blood capillary and permeability improvement, makes cell more Easily absorbing nutrient and get rid of refuse, therefore procyanidin, can press down with water solublity in scavenger cell film and fat-soluble free radical Having made some enzyme of release and gone to injure the process of capillary wall, procyanidin suppression produces the enzyme that histamine needs, prevents generation group Amine, thus dephlogistication；

Herba Portulacae: cold in nature, sweet in the mouth acid, enter the heart, liver, spleen, large intestine channel, have that removing dampness is antipruritic, clearing heat for detumescence, heat-clearing and toxic substances removing, Diuretic dries, and dissipates blood detumescence, dust-collection sterilization, anti-inflammatory analgetic, and the effect of hemostasis removing heat from blood, Herba Portulacae is to dysentery bacterium, Bacillus typhi There is stronger inhibitory action with escherichia coli, can be used for the auxiliary treatment of various inflammation, have the title of " natural antibiotics ", simultaneously Herba Portulacae is also rich in vitamin C, and vitamin C has good antioxidation can be with whitening nourishes rough skin, and modern medicine finds, Herba Portulacae has higher nutritive value and medical value, except containing nutrition units such as conventional proteins,vitamins,and mineralses Outside element, Herba Portulacae is possibly together with unique bioactive ingredients, it is possible to strengthens the activity of Human Epithelial Cells, helps lend some impetus to ulcer And healing；

Radix Sophorae Flavescentis: bitter in the mouth, cold in nature, heat clearing and damp drying, antibacterial parasite killing, diuresis, for hematodiarrhoea, have blood in stool, jaundice urine retention, red and white leukorrhea Under, swelling of the vulva pudendal pruritus, eczema, eczema, skin pruritus, scabies leprosy, external treatment trichomonal vaginitis；

Herba Euphorbiae Humifusae: acrid in the mouth, property puts down, returns liver, large intestine channel, have an effect of heat-clearing and toxic substances removing, cooling blood for hemostasis, damp-heat dysentery, little Just drench pain, jaundice, various go out blood disorder etc. treatment, Herba Euphorbiae Humifusae to staphylococcus aureus, Hemolytic streptococcus, diphtheria corynebacterium, Escherichia coli, dysentery bacterium etc. have the antibacterial or effect of sterilization；

Herba Tagetis Patulae: bitter in the mouth, cool in nature, heat-clearing and toxic substances removing, cough-relieving, main anemopyretic cold, cough, dysentery, rib adenitis, acute mastitis, furuncle and phyma, Toothache, stomatitis, conjunctival congestion and swelling pain；

Myrrha: acrid in the mouth, hardship, property is flat, and dissipating blood stasis analgesic therapy, detumescence and promoting granulation, for obstruction of qi in the chest and cardialgia, gastralgia, dysmenorrhea amenorrhea. produces Rear stasis blocking, abdominal mass stomachache, rheumatic arthralgia, traumatic injury, the treatment of the diseases such as carbuncle skin infection；

Herba Pteridis multifidae: light, cool, clearing away heat-damp and promoting diuresis, hemostasia and promoting granulation, removing toxic substances and promoting subsidence of swelling, cure mainly and have loose bowels, dysentery, jaundice, stranguria, water Swollen, hemoptysis, hematuria, to have blood in stool, knife injury is hemorrhage, treating swelling and pain by traumatic injury, carbuncle sore, burn due to hot liquid or fire；

Flos Matricariae chamomillae hydrosol: can improve acne, allergy, eczema, burn and scald, can say it is one of hydrosol optimal to skin, tool There is anti-inflammation and pacify characteristic, for sensitive and acne vulgaris skin, fabulous, to treatment pimples, bleb to dryness, sensitive skin Rash, eczema, tinea, microvascular have good effect, can be used to the eye skin protecting face most sensitive, for treatment face Skin ulcer and red seal effect are notable, increase skin resistance；

Kochia scoparia: nature and flavor bitter cold, has effect of heat-clearing and toxic substances removing, inducing diuresis for treating stranguria syndrome, control dysentery with red and white feces, have loose bowels, pyretic stranguria, conjunctival congestion, Night blindness, skin wind heat are red swollen, and Kochia scoparia, rich in vitamin C and vitamin B, has fine antioxidation, can protect nourishing Skin；

Entermorpha: China's doctor's mat and experience among the people are thought, large-scale chlorella not only has abundant nutritive value, and has clear Thermal detoxification, anti-inflammation, cholesterol reducing, enhancing human body immunity power, hard masses softening and resolving, swelling diuretic and effect of change disease, according to " with breath Occupy diet spectrum " record: Entermorpha " gallbladder that disappears, the scrofula goiter that disappears, let out swollen, reduce phlegm, control being not acclimatized ", Entermorpha, rich in polysaccharide, accounts for Sargassum Dry weight more than 50%, has biological activity and medicinal function (blood fat reducing, antitumor, antiviral, antibacterial, regulation immunity widely Power, antioxidation, resisting fatigue and blood sugar lowering etc.), can be widely applied to medicine, health food and bread and cheese；

Cortex Mori: sweet, cold, eliminating pathogen from the lung for relieving asthma, inducing diuresis to remove edema, for dyspnea and cough due to lung-heat, edema distension oliguria, appearance skin floats Swollen.

Compared to conventional disinfecting drug, the disinfecting sprays of the present invention uses pure Chinese medicinal preparation, formula safety non-toxic, no Mink skin can be produced and stimulate, effectively prevent mink from eating left drug alignment health by mistake and damage.Selected material combination obtains When, Galla Chinensis, Herba Hedyotidis Diffusae, Herba Taraxaci, mangosteen skin, Radix Sophorae Flavescentis, Herba Euphorbiae Humifusae, Flos Matricariae chamomillae hydrosol, Entermorpha share and can play quickly The effectively effect of bactericidal, the especially Main Pathogenic Bacteria to hemorrhagic pneumonia has stronger bacillus pyocyaneus and kerekou pneumonia primary The inhibitory action of Salmonella, is sprayed in air and can play good ermine house sterilization, the medicinal liquid of atomization in air Effective ingredient is entered in mink body by Repiration, can effectively kill the pathogenic bacterium in mink body, to low-grade infection Mink play prophylactic treatment effect；Meanwhile, Galla Chinensis, Herba Hedyotidis Diffusae, Herba Taraxaci, mangosteen skin, Radix Sophorae Flavescentis, Herba Euphorbiae Humifusae, peafowl Grass, Herba Pteridis multifidae, Kochia scoparia, the effective ingredient of Entermorpha may also operate as the effect of heat-clearing and toxic substances removing in entering mink body by breathing Really, remove damp and hot toxin in mink body, regulate body, strengthen mink autoimmunity, effectively prevent and treat hemorrhagic pneumonia；Mangosteen skin Rich in procyanidin, procyanidin can strengthen blood capillary, tremulous pulse and vein blood vessel, and it has effect that detumescenceization is become silted up, capillary The drag reduction of blood vessel and permeability improve, and make cell be easier to absorb nutrient and get rid of refuse, and procyanidin can be removed carefully Water solublity and fat-soluble free radical in after birth, it is suppressed that discharge some enzyme and remove to injure the process of capillary wall, procyanidin Suppression produces the enzyme that histamine needs, and prevents from generating histamine, thus good blood circulation promoting and blood stasis dispelling is played in dephlogistication, anti-inflammatory analgetic is made With, its with Galla Chinensis, Herba Portulacae, Herba Euphorbiae Humifusae, Myrrha, Herba Pteridis multifidae, Flos Matricariae chamomillae hydrosol, Kochia scoparia etc. raw material rich in vitamin etc. The raw material of nutritional labeling share, and can play and well nourish promoting muscle growth functions, and spray contamination mink skin can effectively nourish protection Skin, will not produce harmful effect to mink skin, and Flos Matricariae chamomillae hydrosol also has good Shu Min effect, can be prevented effectively from medicine Such as Herba Taraxaci etc., skin is produced allergy effect, while ensureing sterilization effect, preferably protect mink skin.

The invention have the benefit that and provide a kind of ermine house air sterillization spray and preparation method thereof, this disinfecting sprays Can effectively suppress the pathogenic bacterium of hemorrhagic pneumonia with express delivery, directly be sprayed in air and can play good sterilizing work With, and formula is safe and reliable, will not produce mink skin and stimulate, and effectively prevents mink from eating left drug alignment health by mistake and causes Injury.Specifically:

(1) compared to conventional disinfecting drug, the disinfecting sprays of the present invention uses pure Chinese medicinal preparation, formula safety non-toxic Property, will not produce mink skin stimulates, and effectively prevents mink from eating left drug alignment health by mistake and damages.Selected raw material is joined 5 is proper, and Galla Chinensis, Herba Hedyotidis Diffusae, Herba Taraxaci, mangosteen skin, Radix Sophorae Flavescentis, Herba Euphorbiae Humifusae, Flos Matricariae chamomillae hydrosol, Entermorpha share and can play The most effectively effect of bactericidal, the especially Main Pathogenic Bacteria to hemorrhagic pneumonia has stronger bacillus pyocyaneus and pneumonia gram The inhibitory action of the primary Salmonella of thunder, is sprayed in air and can play good ermine house sterilization, the medicine of atomization in air The effective ingredient of liquid is entered in mink body by Repiration, can effectively kill the pathogenic bacterium in mink body, to slightly The mink infected plays prophylactic treatment effect；Meanwhile, Galla Chinensis, Herba Hedyotidis Diffusae, Herba Taraxaci, mangosteen skin, Radix Sophorae Flavescentis, Herba Euphorbiae Humifusae, Herba Tagetis Patulae, Herba Pteridis multifidae, Kochia scoparia, the effective ingredient of Entermorpha may also operate as heat-clearing and toxic substances removing in entering mink body by breathing Effect, removes damp and hot toxin in mink body, regulates body, strengthens mink autoimmunity, effectively prevents and treats hemorrhagic pneumonia, Cortex Mori Micromicro priming enters lung, obtains more preferable curative effect；Mangosteen skin can strengthen blood capillary, tremulous pulse rich in procyanidin, procyanidin With vein blood vessel, it has effect that detumescenceization is become silted up, and the drag reduction of blood capillary and permeability improve, and makes cell be easier to absorb Nutrient and eliminating refuse, procyanidin can be with water solublity in scavenger cell film and fat-soluble free radical, it is suppressed that discharge some Enzyme goes to injure the process of capillary wall, and procyanidin suppression produces the enzyme that histamine needs, and prevents from generating histamine, thus alleviates and send out Inflammation plays good blood circulation promoting and blood stasis dispelling, anti-inflammatory analgetic effect, its with Galla Chinensis, Herba Portulacae, Herba Euphorbiae Humifusae, Myrrha, Herba Pteridis multifidae, ocean sweet The raw materials such as chrysanthemum hydrosol, Kochia scoparia share rich in the raw material of vitamins and other nutritious components, can play and well nourish promoting muscle growth functions, Spray contamination mink skin can effectively nourish protection skin, mink skin will not be produced harmful effect, and Flos Matricariae chamomillae hydrosol also has There is good Shu Min effect, medicine such as Herba Taraxaci etc. can be prevented effectively from skin is produced allergy effect, ensure disinfection effect Mink skin is preferably protected while Guo.

(2) in order to protect the effect of the effective ingredient such as vitamin, procyanidin in raw material, the disinfecting sprays of the present invention is in system Use vacuum lyophilization, cryoconcentration technology to extract the effective ingredient of raw material during Bei, fully maintain in raw material and be easily subject to The nutrient substance such as the procyanidin of high temperature, vitamin.

(3) environment will not be caused as raw material, safe without toxic side effect, its residual components by this disinfecting sprays using Chinese herbal medicine Pollute, nuisanceless to human body, there is important economy and social value.

Detailed description of the invention

According to following embodiment, the present invention be may be better understood.But, as it will be easily appreciated by one skilled in the art that reality Execute concrete material proportion, process conditions and result thereof described by example be merely to illustrate the present invention and not should also without limitation on The present invention described in detail in claims.

Embodiment 1

A kind of ermine house air sterillization spray, raw material composition and the parts by weight of making described air spray are: Galla Chinensis 12 Part, Herba Hedyotidis Diffusae 6 parts, Herba Taraxaci 4 parts, peel of Carcinia mangostana L. 6 parts, Herba Portulacae fresh goods 10 parts, Radix Sophorae Flavescentis 7 parts, Herba Euphorbiae Humifusae 2 parts, peafowl Grass 3 parts, Myrrha 2 parts, Herba Pteridis multifidae 4 parts, Flos Matricariae chamomillae hydrosol 15 parts, Kochia scoparia fresh goods 6 parts, Entermorpha fresh goods 8 parts, Cortex Mori 1 part.

The preparation method of a kind of ermine house air sterillization spray, comprises the following steps:

(1) Galla Chinensis, Herba Hedyotidis Diffusae, Herba Taraxaci, Radix Sophorae Flavescentis, Herba Euphorbiae Humifusae, Herba Tagetis Patulae, Myrrha, Herba Pteridis multifidae, Cortex Mori corium farinosum The broken 80 mesh mixed-powders that obtain, the water soaking of gained mixed-powder addition gross mass multiple 5 times 1 hour, slow fire boiling 1 hour, decoct Cross 200 mesh drainage screens after boiling and obtain filtrate, again the water soaking of addition mixed-powder gross mass multiple 5 times 1 hour, slow fire boiling 1 Cross 200 mesh drainage screens after hour and obtain filtrate, merge twice gained filtrate and be cooled to room temperature, standby；

(2) peel of Carcinia mangostana L., Herba Portulacae fresh goods, Kochia scoparia fresh goods, Entermorpha fresh goods are cut into small pieces after cleaning, and add step (1) Gained filtrate grinds pulping after soaking 1 hour, crosses 200 mesh drainage screens and obtain filtrate after standing 3 hours；

(3) step (2) gained filtrate cryoconcentration (less than 40 DEG C) to relative density is the dense of 1.20 ± 0.02 (30 DEG C) Contracting liquid；

(4) after step (3) gained concentrated solution adds Flos Matricariae chamomillae hydrosol mix homogeneously, ultraviolet sterilization 15 minutes, obtains target Thing.

Embodiment 2

A kind of ermine house air sterillization spray, raw material composition and the parts by weight of making described air spray are: Galla Chinensis 16 Part, Herba Hedyotidis Diffusae 8 parts, Herba Taraxaci 6 parts, peel of Carcinia mangostana L. 9 parts, Herba Portulacae fresh goods 15 parts, Radix Sophorae Flavescentis 10 parts, Herba Euphorbiae Humifusae 5 parts, peafowl Grass 5 parts, Myrrha 4 parts, Herba Pteridis multifidae 6 parts, Flos Matricariae chamomillae hydrosol 24 parts, Kochia scoparia fresh goods 9 parts, Entermorpha fresh goods 10 parts, Cortex Mori 3 Part.

Preparation method and the using method of the disinfecting sprays of embodiment 2 are same as in Example 1.

Embodiment 3

A kind of ermine house air sterillization spray, raw material composition and the parts by weight of making described air spray are: Galla Chinensis 14 Part, Herba Hedyotidis Diffusae 7 parts, Herba Taraxaci 5 parts, peel of Carcinia mangostana L. 7 parts, Herba Portulacae fresh goods 13 parts, Radix Sophorae Flavescentis 9 parts, Herba Euphorbiae Humifusae 4 parts, peafowl Grass 4 parts, Myrrha 3 parts, Herba Pteridis multifidae 5 parts, Flos Matricariae chamomillae hydrosol 20 parts, Kochia scoparia fresh goods 7 parts, Entermorpha fresh goods 9 parts, Cortex Mori 2 parts.

Preparation method and the using method of the disinfecting sprays of embodiment 3 are same as in Example 1.

Embodiment 4 hypersensitive test

Take healthy albino guinea-pig 18, body weight 250-300g, male and female dual-purpose, before test, reject both sides dorsal body setae, every side 3 × 3cm, is randomly divided into test group, blank group and positive controls positive controls DNFB, and sensitization is with 1% Concentration, excites and uses 0.1% concentration；The test group disinfecting sprays of the embodiment of the present invention 3.Take the sterilization spray of the embodiment of the present invention 1 Agent is applied to back and goes to hair-fields, the next day 1 time, totally 3 times.Contact latter 14 days in last, smear this disinfecting sprays at back the same area, Remove after medicine after 6h and at once observe, and in 24,48,72h observes with or without anaphylaxis.Blank group and positive controls are used Excite contact with method and observe.Result of the test: positive controls animal skin sensitization i.e. occur after 1 hour localized erythema and Slight edema, within 24 hours, symptoms of allergic increases the weight of, and sensitization rate is 100%, and test group and blank treated animal skin are the most not Occur that any symptoms of allergic, sensitization rate are 0%.Test result indicate that the disinfecting sprays formula gentleness of the present invention is non-stimulated.

Embodiment 5 antibacterial experiment

1, experimental strain: bacillus pyocyaneus (ATCC15442), Klebsiella Pneumoniae (ATCC700603)

2, bacterium culture medium: nutrient agar and nutrient broth medium.

3, bacteriostatic experiment divides three experimental grouies and a matched group, and experimental group uses the sterilization of embodiment 1 to 3 to spray respectively Agent, wherein, experimental group A is the disinfecting sprays of embodiment 1, and experimental group B is the disinfecting sprays of embodiment 2, and experimental group C is embodiment The disinfecting sprays of 3, matched group uses normal saline.

4, specific experiment step:

(1) prepared by susceptibility sense sheet: double-round board qualitative filter paper card punch is made the round scraps of paper of diameter 6mm and put into cleaning and be dried Penicillin empty bottle in, bottleneck is wrapped up with monolayer kraft paper, 121 DEG C of autoclaving 30min, then through 100 DEG C of drying, airtight preservation Standby.The most respectively the scraps of paper are soaked in 2h in the disinfecting sprays of embodiment 1 to 3 and normal saline, treat that liquid is complete After entirely being absorbed by the scraps of paper, taking out filter paper, dry that to make susceptibility sheet standby under room temperature, every kind of liquid prepares 10 susceptibility senses Sheet；

(2) bacterium solution is prepared: bacillus pyocyaneus (ATCC15442), Klebsiella Pneumoniae (ATCC700603) are respectively placed in 10ml In nutrient broth medium, cultivate 18h for 37 DEG C；Take 0.9% aseptic sodium chloride solution that culture fluid 1ml adds 9ml, use 10 times It is incremented by dilution method, bacterium solution is diluted to respectively 105～106cfu/ml, standby；

(3) two kinds of test bacterium solution above-mentioned steps (2) prepared the most uniformly are inoculated in two mannitol sodium chloride agar On flat board, left at room temperature 5min, the 40 susceptibility sense sheets prepared in step (1) are divided into 2 parts, are laid in green pus bar respectively On the agar plate of bacterium inoculation and on the agar plate of Klebsiella Pneumoniae inoculation, flat board is inverted into 37 DEG C of trainings in incubator After supporting 24h, measuring the antibacterial circle diameter (mm) of each susceptibility sheet, calculate the antibacterial circle diameter meansigma methods of each group, above operation is Sterile working.

5, experimental observation is measured:

Observe: represent with "-" without inhibition zone；

Antibacterial circle diameter ＜ 10mm is the most antibacterial, with "+" represent；

Antibacterial circle diameter 10～14mm is that moderate is antibacterial, represents with " ++ "；

Antibacterial circle diameter ＞ 14mm is that height is antibacterial, represents with " +++ "；

	Negative control	Experimental group A	Experimental group B	Experimental group C
					Bacillus pyocyaneus	-	++	++	+++
Klebsiella Pneumoniae	-	++	++	++

Test result indicate that, the air sterillization spray of the present invention Main Pathogenic Bacteria bacillus pyocyaneus to mink hemorrhagic pneumonia All there is with Klebsiella Pneumoniae preferable inhibitory action, preferable bactericidal can be played, prevent mink hemorrhagic pneumonia Effect.

Embodiment 4 toxicity test

The disinfecting sprays of embodiment 1 to 3 is carried out toxicity test, is administered half to mouse stomach and measures (LD till death₅₀) carry out Test determination, but it is not detected by LD₅₀；Carrying out mtd test, result is with Cmax 50%, maximum dosage-feeding 100mg/kg, uses gavage mode to be administered in three times on the 1st, and successive administration 7 days, none is dead for mice, it was demonstrated that the sterilization of the present invention Spray formulations safety non-toxic, will not affect mink health because eating by mistake.

Embodiment of above only for technology design and the feature of the present invention are described, its object is to allow and is familiar with technique People understands present invention and is carried out, and can not limit the scope of the invention with this, all real according to spirit of the present invention The equivalence that matter is done changes or modifies, and all should contain within the scope of the present invention.

Claims (5)

1. ermine house air sterillization spray, it is characterised in that raw material composition and the parts by weight of making described air spray are: Galla Chinensis 12-16 part, Herba Hedyotidis Diffusae 6-8 part, Herba Taraxaci 4-6 part, peel of Carcinia mangostana L. 6-9 part, Herba Portulacae fresh goods 10-15 part, bitter Ginseng 7-10 part, Herba Euphorbiae Humifusae 2-5 part, Herba Tagetis Patulae 3-5 part, Myrrha 2-4 part, Herba Pteridis multifidae 4-6 part, Flos Matricariae chamomillae hydrosol 15-24 part, sweep Broom dish fresh goods 6-9 part, Entermorpha fresh goods 8-10 part, Cortex Mori 1-3 part.

2. a kind of ermine house air sterillization spray as claimed in claim 1, it is characterised in that make the raw material of described air spray Form and parts by weight are: Galla Chinensis 12 parts, Herba Hedyotidis Diffusae 6 parts, Herba Taraxaci 4 parts, peel of Carcinia mangostana L. 6 parts, Herba Portulacae fresh goods 10 Part, Radix Sophorae Flavescentis 7 parts, Herba Euphorbiae Humifusae 2 parts, Herba Tagetis Patulae 3 parts, Myrrha 2 parts, Herba Pteridis multifidae 4 parts, Flos Matricariae chamomillae hydrosol 15 parts, Kochia scoparia fresh goods 6 Part, Entermorpha fresh goods 8 parts, Cortex Mori 1 part.

3. a kind of ermine house air sterillization spray as claimed in claim 1, it is characterised in that make the raw material of described air spray Form and parts by weight are: Galla Chinensis 16 parts, Herba Hedyotidis Diffusae 8 parts, Herba Taraxaci 6 parts, peel of Carcinia mangostana L. 9 parts, Herba Portulacae fresh goods 15 Part, Radix Sophorae Flavescentis 10 parts, Herba Euphorbiae Humifusae 5 parts, Herba Tagetis Patulae 5 parts, Myrrha 4 parts, Herba Pteridis multifidae 6 parts, Flos Matricariae chamomillae hydrosol 24 parts, Kochia scoparia fresh goods 9 parts, Entermorpha fresh goods 10 parts, Cortex Mori 3 parts.

4. a kind of ermine house air sterillization spray as claimed in claim 1, it is characterised in that make the raw material of described air spray Form and parts by weight are: Galla Chinensis 14 parts, Herba Hedyotidis Diffusae 7 parts, Herba Taraxaci 5 parts, peel of Carcinia mangostana L. 7 parts, Herba Portulacae fresh goods 13 Part, Radix Sophorae Flavescentis 9 parts, Herba Euphorbiae Humifusae 4 parts, Herba Tagetis Patulae 4 parts, Myrrha 3 parts, Herba Pteridis multifidae 5 parts, Flos Matricariae chamomillae hydrosol 20 parts, Kochia scoparia fresh goods 7 Part, Entermorpha fresh goods 9 parts, Cortex Mori 2 parts.

5. the preparation method of a kind of ermine house air sterillization spray as described in Claims 1-4, it is characterised in that include following Step:

(1) Galla Chinensis, Herba Hedyotidis Diffusae, Herba Taraxaci, Radix Sophorae Flavescentis, Herba Euphorbiae Humifusae, Herba Tagetis Patulae, Myrrha, Herba Pteridis multifidae, Cortex Mori are pulverized 80 mesh mixed-powders, the water soaking of gained mixed-powder addition gross mass multiple 5 times 1 hour, slow fire boiling 1 hour, after decoction Cross 200 mesh drainage screens and obtain filtrate, again the water soaking of addition mixed-powder gross mass multiple 5 times 1 hour, slow fire boiling 1 hour Rear mistake 200 mesh drainage screen obtains filtrate, merges twice gained filtrate and is cooled to room temperature, standby；

(2) peel of Carcinia mangostana L., Herba Portulacae fresh goods, Kochia scoparia fresh goods, Entermorpha fresh goods are cut into small pieces after cleaning, and add step (1) gained Filtrate grinds pulping after soaking 1 hour, crosses 200 mesh drainage screens and obtain filtrate after standing 3 hours；

(3) step (2) gained filtrate cryoconcentration (less than 40 DEG C) to relative density is the concentrated solution of 1.20 ± 0.02 (30 DEG C)；

(4) after step (3) gained concentrated solution adds Flos Matricariae chamomillae hydrosol mix homogeneously, ultraviolet sterilization 15 minutes, obtains object.