CN106038749B - Composition and its preparation method and application with anti-inflammatory effect - Google Patents
Composition and its preparation method and application with anti-inflammatory effect Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/736—Prunus, e.g. plum, cherry, peach, apricot or almond
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/066—Clavicipitaceae
- A61K36/068—Cordyceps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
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- Engineering & Computer Science (AREA)
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- Pharmacology & Pharmacy (AREA)
- Alternative & Traditional Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a kind of composition and its preparation method and application with anti-inflammatory effect, belongs to natural products technical field.The active constituent of the composition is prepared by the raw material of following weight percent: the Nanking cherry dry product of 40%-60%, the Cordyceps militaris dry product of 60%-40%.Nanking cherry and Cordyceps militaris are cooperated according to aforementioned proportion, the two produces the effect of collaboration, has extraordinary anti-inflammatory effect, also, the natural traditional Chinese medicine of the composition using integration of drinking and medicinal herbs is prepared as raw material, has Small side effects, highly-safe advantage.
Description
Technical field
The present invention relates to natural products technical fields, more particularly to a kind of composition with anti-inflammatory effect and its preparation
Method and purposes.
Background technique
Inflammation is a kind of very common and important basic pathology process, refers to the living tissue with vascular system
To the defense reaction that damage factor is occurred, it is a kind of defense reaction of the body for stimulation, shows as red, swollen, hot, pain and function
It can obstacle.Either infective inflammation caused by infecting, non-infectious inflammation caused by may not be due to infection.
In inflammatory process, one side damage factor is caused directly or indirectly the destruction of tissue and cell, on the other hand passes through
Inflammatory congestion and exudation reaction with dilution, killing and surround damage factor.Simultaneously by essence and the regeneration of interstitial cell make by
The tissue of damage is able to repair and heal.It can be said that inflammation is the unified process of damage and antibody Monoclonal.
Usually, inflammation is a kind of disease resistance response of body, is, such as inflammatory congestion advantageous to body, can make table
Covering weave obtains more oxygen, nutriment and guard substance；Surface texture metabolism and resistance power increase；Diffusate can dilute poison
Element, antibody contained therein can sweep disease carrying germ and neutralize a toxin；The fibronectin condensation of exudation forms pseudomembrane, is set up to one
Road masking can bury pathogen and spread to deep；Neutrocyte and macrophage in exudate, which can swallow, to carry disease germs, moreover it is possible to gulp down
Bite the cell debris for giving up necrosis disintegration；The thick liquid cell and lymphocyte in scorching area can generate antibody and neutralize a toxin；Hyperblastosis energy
Administer defect caused by scorching area.
But we want dialectical and analyze inflammation.In inflammatory reaction it is certain be conducive to factor under certain condition can
To be transformed towards respectively opposite direction to the harmful factor of body.For example, diffusate excessively then when often result in related device
The function of official is hidden, and if pleural effusion can suppress lung, shows difficulty of breathing；Hydropericardium implicates heartbeat, same inflammation evening
Although the connective tissue proliferation and hardening of phase is beneficial to assembling and administers, but set up adhesion or the cirrhosis of parenchymatous organ often,
Seriously implicate the performance of the organ.
Therefore, in clinical practice, generally requiring to carry out anti-inflammatory processing, conventional anti-inflammatory drug has dexamethasone etc., but
It is that the steroids Western medicine such as dexamethasone are used for a long time, a large amount of side effects can be caused, such as iatrogenic Cushing syndrome face and body
State, weight gain, edema of lower extremity, purple striae, easy hemorrhagic tendency, Wound healing be bad, acne, paramenia, the upper arm or femoral head lack
Hemorrhagic necrosis, osteoporosis and fracture, myasthenia, amyotrophia, Diagnostic value syndrome, GI irritation, pancreatitis, digestibility are burst
Ulcer or perforation, children growth is suppressed, glaucoma, cataract, benign intracranial pressure increase syndrome, impaired glucose tolerance and glycosuria
Disease exacerbation etc..
Summary of the invention
Based on this, it is necessary to which, in view of the above-mentioned problems, providing a kind of composition with anti-inflammatory effect, the composition is by natural
Product is prepared, and has the advantages that Small side effects and significant in efficacy.
A kind of composition with anti-inflammatory effect, the active constituent of the composition by following weight percent raw material system
It is standby to form:
Nanking cherry dry product 40%-60%
Cordyceps militaris dry product 60%-40%.
In above-mentioned composition, Nanking cherry (scientific name: Cerasus tomentosa (Thunb.) Wall) is rosaceae, cherry
Belong to, short raw cherry platymiscium, there is anti-inflammatory, analgesia, anticancer, oxidation resistant effect, Cordyceps militaris belongs to careless Hypocreales, ergot
Section, Cordyceps, have the function of tranquilizing soporific, improve immunity of organisms, is antitumor, preventing aging, protection cardiopulmonary tissue etc., it will
Nanking cherry and Cordyceps militaris cooperate according to aforementioned proportion, and the two produces the effect of collaboration, have extraordinary anti-inflammatory effect, and
And the natural products of the composition using integration of drinking and medicinal herbs is prepared as raw material, has Small side effects, highly-safe advantage.
The invention also discloses a kind of preparation methods of above-mentioned composition with anti-inflammatory effect, comprising the following steps:
Extract: predetermined amounts weigh raw material, and the ethanol water that volumn concentration is 80%-100% is added, and reflux mentions
It takes, extracting solution is concentrated as medicinal extract；
Extraction: being taken above-mentioned medicinal extract, be dispersed in water, obtain mother liquor, extracted with ethyl acetate, take ethyl acetate layer extract liquor,
Recycling design, it is dry to get.
Effective component in composition material is enriched with, and pass through by above-mentioned preparation method by way of extracting and extracting
Reduce with the lesser impurity component of active constituent correlation, can reduce composition may to body generate side effect.
In one of the embodiments, in the extraction step, petroleum ether extraction is first used, petroleum ether layer extract is discarded,
Extracted again with ethyl acetate, recycle the solvent in ethyl acetate layer extract liquor, it is dry to get.The present inventor confirms through research, first
Partial impurities are removed with petroleum ether, then are extracted with ethyl acetate, have the effect of preferable active constituent enrichment.
In one of the embodiments, in the extraction step, add according to mother liquor with petroleum ether volume ratio for the amount of 1:3-5
Enter petroleum ether, extracted, extracts 2-4 times, acetic acid second then is added according to the amount that mother liquor and ethyl acetate volume ratio are 1:3-5
Ester is extracted, and is extracted 2-4 times.
In one of the embodiments, in the extraction step, 6-10ml ethanol water is added according to every gram of raw material
The ethanol water that volumn concentration is 90%-100% is added in amount, refluxing extraction 1-3 hours, repeats to extract 2-4 times, merge
Extracting solution is concentrated as medicinal extract extracting solution.
In one of the embodiments, in the extraction step, according to the amount of every gram of raw material 1ml water, the extraction is walked
Suddenly the medicinal extract obtained is dispersed in water.
This law is bright to also disclose a kind of preparation with anti-inflammatory effect, including the combinations of the above object as active constituent,
And pharmaceutically acceptable carrier.
This law is bright to also disclose combinations of the above object in preparation for the application in anti-inflammatory preparation.
The preparation is pharmaceutical preparation or health-care preparation in one of the embodiments,.
The pharmaceutical preparation is oral solution, hard capsule, soft capsule, tablet, pill, dripping pill in one of the embodiments,
Agent, granule, powder, honey ointment agent or distillate medicinal water；The health-care preparation is tea bag or vina.
Customary adjuvant can be used in above-mentioned dosage form and preparation process is prepared.
Compared with prior art, the invention has the following advantages:
A kind of composition with anti-inflammatory effect of the invention cooperates Nanking cherry and Cordyceps militaris, wherein Nanking cherry has
Anti-inflammatory, analgesia, anticancer, oxidation resistant effect, Cordyceps militaris have tranquilizing soporific, improve immunity of organisms, is antitumor, preventing from declining
Always, the effect of cardiopulmonary tissue etc. is protected, the two cooperates according to a certain percentage, produces the effect of collaboration, has extraordinary
Anti-inflammatory effect can significantly reduce mouse ear swelling (p < 0.05).
Also, the natural traditional Chinese medicine of the composition using integration of drinking and medicinal herbs is prepared as raw material, has Small side effects, safety
High advantage.
Specific embodiment
It to facilitate the understanding of the present invention, below will be to invention is more fully described.The present invention is given in attached drawing
Preferred embodiment.But the invention can be realized in many different forms, however it is not limited to implementation described herein
Example.It is made the disclosure of the present invention more thorough and comprehensive on the contrary, purpose of providing these embodiments is.
Unless otherwise defined, all technical and scientific terms used herein and belong to technical field of the invention
The normally understood meaning of technical staff is identical.Term as used herein in the specification of the present invention is intended merely to description tool
The purpose of the embodiment of body, it is not intended that in the limitation present invention.
Embodiment 1
A kind of composition with anti-inflammatory effect, is prepared by the following method to obtain:
One, prepare raw material.
The full fruit of Nanking cherry is put into 60 DEG C of baking ovens, takes out, is cooled to room temperature after drying 24~48h.The Nanking cherry of drying is taken to put
Enter and crushed in grinder, beading shape is to get Nanking cherry dry product.
Cordyceps militaris is put into 60 DEG C of baking ovens, takes out, is cooled to room temperature after drying 24~48h.The Cordyceps militaris of drying is taken to be put into powder
It is crushed in millstone, beading shape is to get Cordyceps militaris dry product.
Two, it extracts.
100g Nanking cherry dry product and 100g Cordyceps militaris dry product are taken respectively, is placed in 2000ml round-bottomed flask, according to every gram of original
The amount (i.e. solid-liquid ratio is 1:8) of 8ml ethanol water is added in material, and the ethanol water that volumn concentration is 95% is added
1600ml, refluxing extraction 2h, in triplicate, filtering, combined extract are concentrated under reduced pressure into medicinal extract.
Three, it extracts.
Above-mentioned medicinal extract is taken, is distributed in 200ml water, mother liquor is obtained, respectively with 800ml petroleum ether (mother liquor and petroleum ether volume
Than for 1:4), 800ml ethyl acetate (mother liquor and ethyl acetate volume ratio be 1:4) extraction three times (each dosage is 800ml), extracts
After taking liquid and remaining water layer to be concentrated under reduced pressure respectively, it is put into (40-50 DEG C) of baking oven drying, obtains three kinds of mixtures, respectively petroleum
Ether layer extract, ethyl acetate layer extract A and water layer extract.
Comparative example 1
A kind of composition is prepared by Nanking cherry and Chinese yam according to the method for embodiment 1, compared with Example 1, only
It is that Cordyceps militaris is replaced with into Chinese yam, remaining operating procedure and method obtain ethyl acetate layer extract B with embodiment 1.
Comparative example 2
A kind of composition is prepared by Nanking cherry according to the method for embodiment 1, and compared with Example 1, being only will be former
Material replaces with a kind of raw material of Nanking cherry by the dry product mixture of Nanking cherry and Cordyceps militaris, remaining operating procedure and method are the same as implementation
Example 1 obtains ethyl acetate layer extract C.
Comparative example 3
A kind of composition is prepared by Cordyceps militaris according to the method for embodiment 1, and compared with Example 1, being only will be former
Material replaces with a kind of raw material of Cordyceps militaris by the dry product mixture of Nanking cherry and Cordyceps militaris, remaining operating procedure and method are the same as implementation
Example 1 obtains ethyl acetate layer extract D.
Experimental example
The extract that above-described embodiment and comparative example are prepared carries out anti-inflammatory experiment, and the specific method is as follows:
One, method.
1, it is administered.
Taking weight is the mouse of 18-22g, random to be grouped, and is divided into positive group (giving dexamethasone), negative group (is given life
Manage salt water), water layer extract group, ethyl acetate layer extract A group, petroleum ether layer extract group, ethyl acetate layer extract B
Group, ethyl acetate layer extract C group, ethyl acetate layer extract D group, every group of 10 mouse.
Respectively according to the dose subcutaneous drug administration by injection of 10mg/20g 5 days.
2, anti-inflammation test.
(1), croton oil is prepared.
Croton oil prescription: 2% croton oil, 20% dehydrated alcohol, 78% ether (volume ratio) are prepared.
(2), method.
Mouse is administered the 5th day, 1h after administration, and croton oil is applied to the left ear of mouse, and dosage is 20 μ l/, and auris dextra is made
For reference.After 4h, mouse is put to death, clip mouse left-right ear is punched respectively in left and right ear same area with electric perforator, taken
Under ear tissue of the same area, weighing calculates swelling.
(3) result.
Swelling is calculated according to the following equation:
Concrete outcome is as shown in the table:
1. mouse ear swelling result of table
| Group | Swelling (%) |
| Negative group | 91.88±24.81 |
| Positive group | 20.85±10.1* |
| Water layer extract group | 83.96±39.5 |
| Ethyl acetate layer extract A group | 42.36±20.18* |
| Petroleum ether layer extract group | 78.93±21.82 |
| Ethyl acetate layer extract B group (Nanking cherry+Chinese yam) | 66.93±21.52 |
| Ethyl acetate layer extract C group (Nanking cherry) | 69.16±23.1 |
| Ethyl acetate layer extract D group (Cordyceps militaris) | 72.36±17.08 |
Note: above-mentioned swelling is swelling mean+SD (SD).
* indicate that compared with negative group, there is statistical difference, p < 0.05.
As can be seen that the ear swelling of negative control group is 91.88 ± 24.81% in from the above, illustrate croton oil
It can actually cause Mice Auricle inflammatory reaction, modeling success；Positive controls are 20.85 ± 10.10%, organize phase with feminine gender
Than positive drug can generate inhibiting effect to Mice Auricle inflammatory reaction caused by croton oil.
In swelling result, water layer extract group is 83.96 ± 39.50%, and ethyl acetate layer extract A group is 42.36
± 20.18%, petroleum ether layer extract group be 78.93 ± 21.82%, ethyl acetate layer extract B group be 66.93 ±
21.52%, ethyl acetate layer extract C group is 69.16 ± 23.1%, ethyl acetate layer extract D group for 72.36 ±
17.08%, illustrate compared with negative group, said extracted object has the effect of Mice Auricle inflammatory reaction caused by inhibiting croton oil
Fruit, still, wherein ethyl acetate layer extract A has optimal inhibitory effect.
Also, through statistical analysis, positive controls (dexamethasone) and ethyl acetate layer extract A group can significantly drop
Low mouse ear swelling degree (p < 0.05).
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (9)
1. a kind of composition with anti-inflammatory effect, which is characterized in that the active constituent of the composition is by following weight percent
The raw material of ratio is prepared:
Nanking cherry dry product 40%-60%
Cordyceps militaris dry product 60%-40%；
The composition is prepared by the following method to obtain:
Extract: predetermined amounts weigh raw material, are added the ethanol water that volumn concentration is 80%-100%, refluxing extraction,
Extracting solution is concentrated as medicinal extract；
Extraction: taking above-mentioned medicinal extract, be dispersed in water, obtain mother liquor, first uses petroleum ether extraction, discards petroleum ether layer extract, then with
Ethyl acetate extraction, takes ethyl acetate layer extract liquor, recycling design, it is dry to get.
2. the preparation method of the composition according to claim 1 with anti-inflammatory effect, which is characterized in that including following step
It is rapid:
Extract: predetermined amounts weigh raw material, are added the ethanol water that volumn concentration is 80%-100%, refluxing extraction,
Extracting solution is concentrated as medicinal extract；
Extraction: taking above-mentioned medicinal extract, be dispersed in water, obtain mother liquor, first uses petroleum ether extraction, discards petroleum ether layer extract, then with
Ethyl acetate extraction, takes ethyl acetate layer extract liquor, recycling design, it is dry to get.
3. the preparation method of the composition according to claim 2 with anti-inflammatory effect, which is characterized in that the extraction step
In rapid, petroleum ether is added according to the amount that mother liquor and petroleum ether volume ratio are 1:3-5, is extracted, extract 2-4 times, then according to
Ethyl acetate is added in the amount that mother liquor and ethyl acetate volume ratio are 1:3-5, is extracted, and extracts 2-4 times.
4. the preparation method of the composition according to claim 2 with anti-inflammatory effect, which is characterized in that the extraction step
In rapid, the ethanol water that volumn concentration is 90%-100% is added according to the amount that 6-10ml ethanol water is added in every gram of raw material
Solution refluxing extraction 1-3 hours, repeats to extract 2-4 times, extracting solution is concentrated as medicinal extract combined extract.
5. the preparation method of the composition according to claim 2 with anti-inflammatory effect, which is characterized in that the extraction step
In rapid, according to the amount of every gram of raw material 1ml water, the medicinal extract that the extraction step obtains is dispersed in water.
6. a kind of preparation with anti-inflammatory effect, which is characterized in that including the combination described in claim 1 as active constituent
Object and pharmaceutically acceptable carrier.
7. composition described in claim 1 is in preparation for the application in anti-inflammatory preparation.
8. application according to claim 7, which is characterized in that the preparation is pharmaceutical preparation or health-care preparation.
9. application according to claim 8, which is characterized in that the pharmaceutical preparation be oral solution, hard capsule, soft capsule,
Tablet, pill, pill, granule, powder, honey ointment agent or distillate medicinal water；The health-care preparation is tea bag or vina.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1990014A (en) * | 2005-12-26 | 2007-07-04 | 富阳 | Cordyceps militaris fat expelling element and process for manufacturing same |
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2016
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1990014A (en) * | 2005-12-26 | 2007-07-04 | 富阳 | Cordyceps militaris fat expelling element and process for manufacturing same |
Non-Patent Citations (1)
| Title |
|---|
| "毛樱桃乙醇提取物的试验研究";孙晶波等;《中国实验方剂学杂质》;20100831;第16卷(第10期);第33-35页 |
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